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Sample preparation for scanning electron microscopy

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https://www.readbyqxmd.com/read/28639437/ultrasound-assisted-green-economic-synthesis-of-hydroxyapatite-nanoparticles-using-eggshell-bio-waste-and-study-of-mechanical-and-biological-properties-for-orthopaedic-applications
#1
Vijay H Ingole, Kamal Hany Hussein, Anil A Kashale, Kalyani Ghule, Tomaž Vuherer, Vanja Kokol, Jia-Yaw Chang, Yong-Chien Ling, Aruna Vinchurkar, Hom N Dhakal, Anil V Ghule
Nanostructured hydroxyapatite (HAp) is most favorable candidate biomaterial for bone tissue engineering because of its bioactive and osteoconductive properties. Herein, we report for the first time ultrasound assisted facile and economic approach for the synthesis of nanocrystalline hydroxyapatite (Ca10 (PO4 )6 (OH)2 ) using recycled eggshell bio-waste referred as EHAp. The process involves the reaction of eggshell bio-waste as a source of calcium and ammonium dihydrogen orthophosphate as a phosphate source...
June 22, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28636123/sem-imaging-of-the-stimulatory-response-of-raw264-7-cells-against-porphyromonas-gingivalis-using-a-simple-technique-employing-new-conductive-materials
#2
Chisato Takahashi, Yoshiki Umemura, Ayako Naka, Hiromitsu Yamamoto
In the medical biology, it is essential to understand not only biological morphology but also the interaction between biological materials and agents. To study these, electron microscopy (EM) is often utilized. However, sample preparation techniques for EM require a high level of skill and a considerable time. Here, we conducted EM using a simple technique employing a conductive liquid, BEL-1, and compared the results with another simple technique employing an ionic liquid, 1-butyl-3-methylimidazolium tetrafluoroborate ([BMIM][BF4 ])...
June 21, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28629087/a-novel-candidate-for-wound-dressing-transparent-porous-maghemite-cellulose-nanocomposite-membranes-with-controlled-release-of-doxorubicin-from-a-simple-approach
#3
Hao Zhang, Xiaogang Luo, Hu Tang, Mingming Zheng, Fenghong Huang
The aim of this study is to develop transparent maghemite/cellulose nanocomposite membranes with high porosity, high adsorption capacity and controlled release of doxorubicin to be used as a candidate for wound dressing. The membranes were fabricated by a tape casting method through blending a homogeneous dispersion of citrate coated maghemite nanoparticles and cellulose in the NaOH/urea aqueous solution system. The prepared membranes were characterized by Light transmittance measurements, Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray Diffractometry (XRD), Differential scanning calorimetry (DSC), Tensile tests and Vibrating sample magnetometer (VSM)...
October 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28629051/novel-biomimetic-composite-material-for-potentiometric-screening-of-acetylcholine-a-neurotransmitter-in-alzheimer-s-disease
#4
Ana S Sacramento, Felismina T C Moreira, Joana L Guerreiro, Ana P Tavares, M Goreti F Sales
This work describes a novel approach to produce an antibody-like biomimetic material. It includes preparing composite imprinted material never presented before, with highly conductive support nanostructures and assembling a high conductivity polymeric layer at low temperature. Overall, such highly conductive material may enhance the final features of electrically-based devices. Acetylcholine (ACh) was selected as target analyte, a neurotransmitter of importance in Alzheimer's disease. Potentiometric transduction was preferred, allowing quick responses and future adaptation to point-of-care requirements...
October 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28623691/quantification-of-fenestrations-in-liver-sinusoidal-endothelial-cells-by-atomic-force-microscopy
#5
Bartlomiej Zapotoczny, Karolina Szafranska, Edyta Kus, Stefan Chlopicki, Marek Szymonski
Liver sinusoidal endothelial cells present unique morphology characterized by the presence of transmembrane pores called fenestrations. The size and number of fenestrations in live cells change dynamically in response to variety of chemical and physical factors. Although scanning electron microscopy is a well-established method for investigation of fixed liver sinusoidal endothelial cells morphology, atomic force microscopy is the interesting alternative providing detailed 3D topographical information. Moreover, simple sample preparation, only by wet-fixation, minimizing sample preparation artifacts enable high-resolution atomic force microscopy-based measurements...
June 10, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/28622562/hierarchical-flower-like-nickel-ii-oxide-microspheres-with-high-adsorption-capacity-of-congo-red-in-water
#6
Yingqiu Zheng, Bicheng Zhu, Hua Chen, Wei You, Chuanjia Jiang, Jiaguo Yu
Monodispersed hierarchical flower-like nickel(II) oxide (NiO) microspheres were fabricated by a facile solvothermal reaction with the assistance of ethanolamine and a subsequent calcination process. The as-synthesized samples were characterized by X-ray diffraction, scanning electron microscopy, transmission electron microscopy, nitrogen adsorption-desorption isotherms, zeta potential measurement and Fourier transform infrared spectroscopy. Flower-like nickel(II) hydroxide microspheres with uniform diameters of approximate 6...
June 13, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28613819/a-facile-all-solution-processed-surface-with-high-water-contact-angle-and-high-water-adhesive-force
#7
Mei Chen, Wei Hu, Xiao Liang, Cheng Zou, Fasheng Li, Lanying Zhang, Feiwu Chen, Huai Yang
A series of sticky superhydrophobicity surfaces with high water contact angle and high water adhesive force is facilely prepared via an all-solution-processed method based on polymerization-induced phase separation between liquid crystals (LCs) and epoxy resin, which produces layers of epoxy microspheres (EMSs) with nanofolds on the surface of a substrate. The morphologies and size distributions of EMSs are confirmed by scanning electron microscopy. Results reveal that the obtained EMS coated-surface exhibits high apparent contact angle of 152...
June 27, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28610486/resveratrol-loaded-glycyrrhizic-acid-conjugated-human-serum-albumin-nanoparticles-wrapping-resveratrol-nanoparticles-preparation-characterization-and-targeting-effect-on-liver-tumors
#8
Mingfang Wu, Bolin Lian, Yiping Deng, Ziqi Feng, Chen Zhong, Weiwei Wu, Yannian Huang, Lingling Wang, Chang Zu, Xiuhua Zhao
In this study, glycyrrhizic acid-conjugated human serum albumin nanoparticles wrapping resveratrol nanoparticles were prepared to establish a tumor targeting nano-sized drug delivery system. Glycyrrhizic acid was coupled to human serum albumin, and resveratrol was encapsulated in glycyrrhizic acid-conjugated human serum albumin by high-pressure homogenization emulsification. The average particle size of sample nanoparticles prepared under the optimal conditions was 108.1 ± 5.3 nm with a polydispersity index (PDI) of 0...
January 1, 2017: Journal of Biomaterials Applications
https://www.readbyqxmd.com/read/28609745/bacteria-immobilized-electrospun-polycaprolactone-and-polylactic-acid-fibrous-webs-for-remediation-of-textile-dyes-in-water
#9
Omer Faruk Sarioglu, Nalan Oya San Keskin, Asli Celebioglu, Turgay Tekinay, Tamer Uyar
In this study, preparation and application of novel biocomposite materials for textile dye removal which are produced by immobilization of specific bacteria onto electrospun nanofibrous webs are presented. A textile dye remediating bacterial isolate, Clavibacter michiganensis, was selected for bacterial immobilization, a commercial reactive textile dye, Setazol Blue BRF-X, was selected as the target contaminant, and electrospun polycaprolactone (PCL) and polylactic acid (PLA) nanofibrous polymeric webs were selected for bacterial integration...
June 6, 2017: Chemosphere
https://www.readbyqxmd.com/read/28609726/removal-of-tetracycline-from-aqueous-solution-by-mcm-41-zeolite-a-loaded-nano-zero-valent-iron-synthesis-characteristic-adsorption-performance-and-mechanism
#10
Yige Guo, Wenli Huang, Bin Chen, Ying Zhao, Dongfang Liu, Yu Sun, Bin Gong
In this study, nano zero valent iron (NZVI) modified MCM-41-zeolite A (Fe-MCM-41-A) composite as a novel adsorbent was prepared by precipitation method and applied for tetracycline (TC) removal from aqueous solution. The adsorbent was characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared (FT-IR) spectroscopy, X-ray photoelectron spectroscopy (XPS) and N2-BET analysis. Hysteresis loops indicated that the sample has a desirable magnetic property and can be separated quickly...
June 6, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28609725/new-insights-on-mitochondrial-heterogeneity-observed-in-prepared-mitochondrial-samples-following-a-method-for-freeze-fracture-and-scanning-electron-microscopy
#11
Julie A MacDonald, William H Fowle, Dori C Woods PhD
Mitochondria are dynamic intracellular organelles with diverse roles in tissue- and cell type-specific processes, extending beyond bioenergetics. In keeping with this array of functions, mitochondria are described as heterogeneous both between and within tissue types based on multiple parameters, including a broad spectrum of morphological features, and new research points toward a need for the evaluation of mitochondria as isolated organelles. Although transmission electron microscopy (TEM) is commonly used for the evaluation of mitochondria in tissues and renders mitochondrial structures in ultra-thin sections in two-dimensions, additional information regarding complex features within these organelles can be ascertained using scanning electron microscopy (SEM), which allows for analysis of phenotypic differences in three-dimensions...
June 1, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/28608810/microcontact-imprinted-plasmonic-nanosensors-powerful-tools-in-the-detection-of-salmonella-paratyphi
#12
Işık Perçin, Neslihan Idil, Monireh Bakhshpour, Erkut Yılmaz, Bo Mattiasson, Adil Denizli
Identification of pathogenic microorganisms by traditional methods is slow and cumbersome. Therefore, the focus today is on developing new and quicker analytical methods. In this study, a Surface Plasmon Resonance (SPR) sensor with a microcontact imprinted sensor chip was developed for detecting Salmonella paratyphi. For this purpose, the stamps of the target microorganism were prepared and then, microcontact S. paratyphi-imprinted SPR chips were prepared with the functional monomer N-methacryloyl-L-histidine methyl ester (MAH)...
June 13, 2017: Sensors
https://www.readbyqxmd.com/read/28605112/candida-albicans-biofilms-comparative-analysis-of-room-temperature-and-cryofixation-for-scanning-electron-microscopy
#13
T Vila, B B Fonseca, M M L DA Cunha, G R C Dos Santos, K Ishida, E Barreto-Bergter, W DE Souza, S Rozental
Biofilms are frequently related to invasive fungal infections and are reported to be more resistant to antifungal drugs than planktonic cells. The structural complexity of the biofilm as well as the presence of a polymeric extracellular matrix (ECM) is thought to be associated with this resistant behavior. Scanning electron microscopy (SEM) after room temperature glutaraldehyde-based fixation, have been used to study fungal biofilm structure and drug susceptibility but they usually fail to preserve the ECM and, therefore, are not an optimised methodology to understand the complexity of the fungal biofilm...
June 12, 2017: Journal of Microscopy
https://www.readbyqxmd.com/read/28602299/fabrication-of-polymeric-ionic-liquid-modified-magnetic-adsorbent-for-extraction-of-apolar-and-polar-pollutants-in-complicated-samples
#14
Cheng Liu, Yingmin Liao, Xiaojia Huang
A new polymeric ionic liquid-modified magnetic adsorbent (PIL-MA) was successfully fabricated and used to extract apolar and polar pollutants with magnetic solid-phase extraction (MSPE). The PIL-MA was prepared by simple free radical copolymerization of 1-vinylbenzyl-3-methylimidazolium hexafluorophosphate, divinylbenzene and silica-coated magnetite. Several characterized techniques including infrared spectroscopy, elemental analysis, scanning electron microscopy, transmission electron microscopy and magnetic measurement were used to characterize the PIL-MA...
September 1, 2017: Talanta
https://www.readbyqxmd.com/read/28600562/influence-of-w-substitution-on-crystal-structure-phase-evolution-and-microwave-dielectric-properties-of-na0-5bi0-5-moo4-ceramics-with-low-sintering-temperature
#15
Li-Xia Pang, Di Zhou, Ze-Ming Qi, Zhen-Xing Yue
In this work, the (Na0.5Bi0.5)(Mo1-xWx)O4 (x = 0.0, 0.5 and 1.0) ceramics were prepared via solid state reaction method. All the samples can be well densified at sintering temperature about ~720 °C. Dense and homogeneous microstructure with grain size lying between 2~8 μm can be observed from scanning electron microscopy (SEM). Microwave dielectric permittivity of the (Na0.5Bi0.5)(Mo0.5W0.5)O4 ceramic was found to be temperature-independent in a wide range between 25~120 °C with a temperature coefficient of frequency (TCF) ~-6 ppm/°C, a permittivity ~28...
June 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28599176/a-highly-sensitive-immunosensor-based-on-ito-thin-films-covered-by-a-new-semi-conductive-conjugated-polymer-for-the-determination-of-tnf%C3%AE-in-human-saliva-and-serum-samples
#16
Elif Burcu Aydın, Muhammet Aydın, Mustafa Kemal Sezgintürk
A novel, ultrasensitive impedimetric immunosensor was constructed for the detection of tumor necrosis factor α (TNFα) by using Poly(3-thiophene acetic acid) (P3), a conjugated polymer as an immobilization matrix. The polymer P3 contains a lot of carboxylic acid groups on its surface that provide a larger biorecognition surface. This developed immunosensor was prepared on hydroxy-bearing ITO surface via an ester bond linkage of polymer P3 to immobilize anti-TNF α antibodies. The ITO electrode modification steps and interaction between anti-TNF α antibody and TNF α antigen were monitored by cyclic voltammetry (CV) and by electrochemical impedance spectroscopy (EIS) method...
June 2, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28592914/size-controlled-gold-nanoparticles-on-octahedral-anatase-particles-as-efficient-plasmonic-photocatalyst
#17
Zhishun Wei, Lorenzo Rosa, Kunlei Wang, Maya Endo, Saulius Juodkazis, Bunsho Ohtani, Ewa Kowalska
Octahedral anatase particles (OAPs), prepared by ultrasonication-hydrothermal reaction (US-HT), were modified with 2 wt% of gold by photodeposition. Conditions of US-HT process such as durations of US and durations of HT were varied to obtain OAPs products different by physicochemical and morphological properties. Au/OAPs samples were characterized by X-ray diffraction (XRD), scanning transmission electron microscopy (STEM), X-ray photoelectron spectroscopy (XPS) and diffuse reflectance spectroscopy (DRS)...
June 5, 2017: Applied Catalysis. B, Environmental
https://www.readbyqxmd.com/read/28592817/imaging-gold-nanoparticles-in-mouse-liver-by-laser-ablation-inductively-coupled-plasma-mass-spectrometry
#18
Qing Li, Zheng Wang, Jiamei Mo, Guoxia Zhang, Yirui Chen, Chuchu Huang
Imaging the size distribution of metal nanoparticles (NPs) in a tissue has important implications in terms of evaluating NP toxicity. Microscopy techniques used to image tissue NPs are limited by complicated sample preparation or poor resolution. In this study, we developed a laser ablation (LA) system coupled to single-particle inductively coupled plasma mass spectrometry (SP-ICP-MS) for quantitative imaging of gold (G)NPs in tissue samples. In this system, GNPs were ablated but did not disintegrate and integrate under optimised operation conditions, which were verified by characterising LA particles by scanning electron microscopy...
June 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28575946/microwave-assisted-extraction-as-an-efficient-approach-for-biosynthesis-of-zinc-oxide-nanoparticles-synthesis-characterization-and-biological-properties
#19
Hamid Reza Rajabi, Reza Naghiha, Mansoureh Kheirizadeh, Hamed Sadatfaraji, Ali Mirzaei, Zinab Moradi Alvand
In the present study, microwave assisted extraction (MAE) was applied as an efficient, green and rapid approach to prepare the aqueous extract of Suaeda aegyptiaca (SA) plant. The obtained aqueous extracts at two different irradiation power (90 and 270W; 15min) in MAE process as well as maceration method (24h) were used in a green and eco-friendly approach for the synthesis of zinc oxide nanoparticles (ZnO NPs). The synthesized ZnO NPs have been characterized via different techniques including UV-Vis absorption; fluorescence and Fourier transform infrared (FT-IR) spectroscopices, scanning electron microscopy (SEM), and X-ray diffraction (XRD)...
September 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28574450/stable-abts-immobilized-in-the-mil-100-fe-metal-organic-framework-as-an-efficient-mediator-for-laccase-catalyzed-decolorization
#20
Youxun Liu, Yuanyuan Geng, Mingyang Yan, Juan Huang
The successful encapsulation of 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), a well-known laccase mediator, within a mesoporous metal-organic framework sample (i.e., MIL-100(Fe)) was achieved using a one-pot hydrothermal synthetic method. The as-prepared ABTS@MIL-100(Fe) was characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier transform infrared (FT-IR) spectroscopy, nitrogen sorption, and cyclic voltammetry (CV). Our ABTS@MIL-100(Fe)-based electrode exhibited an excellent electrochemical response, indicating that MIL-100(Fe) provides an appropriate microenvironment for the immobilization and electroactivity of ABTS molecules...
June 2, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
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