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Sample preparation for scanning electron microscopy

Fozia Shaheen, Muhammad Hammad Aziz, Muhammad Fakhar-E-Alam, Muhammad Atif, Mahvish Fatima, Riaz Ahmad, Atif Hanif, Saqib Anwar, Fatima Zafar, Ghazanfar Abbas, Syed Mansoor Ali, Mukhtar Ahmed
Graphene-based materials have garnered significant attention because of their versatile bioapplications and extraordinary properties. Graphene oxide (GO) is an extremely oxidized form of graphene accompanied by the functional groups of oxygen on its surface. GO is an outstanding platform on which to pacify silver nanoparticles (Ag NPs), which gives rise to the graphene oxide-silver nanoparticle (GO-Ag) nanocomposite. In this experimental study, the toxicity of graphene oxide-silver (GO-Ag) nanocomposites was assessed in an in vitro human breast cancer model to optimize the parameters of photodynamic therapy...
November 21, 2017: Nanomaterials
Camilla Corrêa Silva, Vivian Maria Durange Ferreira, Gustavo De-Deus, Daniel Rodrigo Herrera, Maíra do Prado, Emmanuel João Nogueira Leal da Silva
This study compared the effect of intermediate flush with distilled water delivered by conventional irrigation, EndoVac microcannula or Self-Adjusting File (SAF) system in the prevention of chemical smear layer (CSL) formation. Thirty human premolars were used. Canals were prepared with Reciproc system and 5.25% NaOCl. After chemomechanical preparation, samples were divided in 3 groups (n=10) according to the intermediate irrigation protocol with distilled water using: conventional irrigation, EndoVac microcannula or SAF...
July 2017: Brazilian Dental Journal
Bipin Gaihre, Beata Lecka-Czernik, Ambalangodage C Jayasuriya
This study was aimed at assessing the effects of silica nanopowder incorporation into chitosan-tripolyphosphate microparticles with the ultimate goal of improving their osteogenic properties. The microparticles were prepared by simple coacervation technique and silica nanopowder was added at 0% (C), 2.5% (S1), 5% (S2) and 10% (S3) (w/w) to chitosan. We observed that this simple incorporation of silica nanopowder improved the growth and proliferation of osteoblasts along the surface of the microparticles. In addition, the composite microparticles also showed the increased expression of alkaline phosphatase and osteoblast specific genes...
January 1, 2017: Journal of Biomaterials Applications
Fahad Naeem, Samiullah Khan, Aamir Jalil, Nazar Muhammad Ranjha, Amina Riaz, Malik Salman Haider, Shoaib Sarwar, Fareha Saher, Samrin Afzal
Introduction: The current work was aimed to design and synthesize novel crosslinked pH-sensitive gelatin/pectin (Ge/Pec) hydrogels using different polymeric ratios and to explore the effect of polymers and degree of crosslinking on dynamic, equilibrium swelling and in vitro release behavior of the model drug (Mannitol). Methods: The Ge/Pec based hydrogels were prepared using glutaraldehyde as the crosslinker. Various structural parameters that affect their release behavior were determined, including swelling study, porosity, sol-gel analysis, average molecular weight between crosslinks (Mc), volume fraction of polymer (V2,s), solvent interaction parameter (χ) and diffusion coefficient...
2017: BioImpacts: BI
Najing Fu, Liteng Li, Xiao Liu, Nian Fu, Chenchen Zhang, Liandong Hu, Donghao Li, Baokun Tang, Tao Zhu
Typically, a target compound is selected as a template for a molecularly imprinted polymer (MIP); however, some target compounds are not suitable as templates because of their poor solubility. Using the tailoring properties of a deep eutectic solvent (DES), the insoluble target compound caffeic acid was transformed into a ternary choline chloride-caffeic acid-ethylene glycol (ChCl-CA-EG) DES, which was then employed as a template to prepare MIPs. The ternary DES-based MIPs were characterized by Fourier transform infrared spectroscopy, elemental analysis, scanning electron microscopy, and atomic force microscopy...
November 7, 2017: Journal of Chromatography. A
Hugo A Vidotti, Ricardo M Carvalho, Paulo G Coelho, Willian F Zambuzzi, Gerson Bonfante, Accácio L do Valle, Estevam A Bonfante
PURPOSE: To examine the effect of mechanical fatigue on the bond strength of resin composite cemented to silicacoated yttria-tetragonal zirconia polycrystal ceramic (Y-TZP). MATERIALS AND METHODS: Ten Y-TZP blocks were polished down to 600-grit silicon carbide paper. Specimens were silica coated by airborne-particle abrasion with 30-μm silica-modified Al2O3 particles. Blocks were cleaned in an ultrasonic bath, and a dental adhesive was applied and light cured for 20 s...
November 17, 2017: Journal of Adhesive Dentistry
Guillaume Wille, Jennifer Hellal, Patrick Ollivier, Annie Richard, Agnes Burel, Louis Jolly, Marc Crampon, Caroline Michel
Understanding biofilm interactions with surrounding substratum and pollutants/particles can benefit from the application of existing microscopy tools. Using the example of biofilm interactions with zero-valent iron nanoparticles (nZVI), this study aims to apply various approaches in biofilm preparation and labeling for fluorescent or electron microscopy and energy dispersive X-ray spectrometry (EDS) microanalysis for accurate observations. According to the targeted microscopy method, biofilms were sampled as flocs or attached biofilm, submitted to labeling using 4',6-diamidino-2-phenylindol, lectins PNA and ConA coupled to fluorescent dye or gold nanoparticles, and prepared for observation (fixation, cross-section, freezing, ultramicrotomy)...
November 16, 2017: Microscopy and Microanalysis
Alireza Khataee, Peyman Gholami, Dimitrios Kalderis, Eleni Pachatouridou, Michalis Konsolakis
The sonocatalytic performance of CeO2 nanoparticles synthesized by a hydrothermal method (CeO2-H) and CeO2@biochar (CeO2-H@BC) nanocomposite, were evaluated for the degradation of Reactive Red 84 (RR84) under ultrasonic irradiation. For comparison purposes the corresponding performance of bare biochar (BC) and commercial CeO2 (CeO2-C) samples were also assessed. A complementary characterization study, involving scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), Transmission electron microscopy (TEM), dynamic light scattering (DLS), X-ray diffraction (XRD), N2 adsorption at -196°C (Brunauer-Emmett-Teller (BET) method) and Fourier transform infrared spectroscopy (FT-IR) was undertaken to gain insight into the structure-performance relationships...
March 2018: Ultrasonics Sonochemistry
Zahra Sharifalhoseini, Mohammad H Entezari, Mohsen Shahidi
The several types of sonication methods were applied to access the different morphologies of ZnO nanostructures on the surface of mild steel. To achieve this goal, a sonictor equipped with the probe extender was used as a high intensity ultrasonic apparatus for direct sonication. Furthermore, an ultrasonic bath (low intensity) and a cup-horn system (high intensity) were applied for indirect sonication. To find the effect of the acoustic waves on the ZnO morphology, the micrographs of obtained surfaces were compared to the sample prepared by the conventional method using scanning electron microscopy (SEM)...
March 2018: Ultrasonics Sonochemistry
Shadpour Mallakpour, Zahra Hajjari
This paper describes the preparation of poly(vinyl chloride) (PVC) nanocomposites (NCs) reinforced with modified zirconia (ZrO2) nanoparticles (NPs). The ZrO2 NPs were defined as efficient filler for PVC NCs. For achieving the best dispersion and improvement of properties, the surface of ZrO2 NPs was modified by Bovine Serum Albumin (BSA). Carboxylic acids and amines are important functional groups of BSA which handle the grafting BSA on the surface of ZrO2 NPs. The PVC/ZrO2-BSA NCs were fabricated by incorporation of various amounts of the ZrO2-BSA NPs (3, 6 and 9wt%) into PVC matrix...
March 2018: Ultrasonics Sonochemistry
Xin-Xin Li, Li-Shun Zhang, Chao Wang, Yan-Ping Huang, Zhao-Sheng Liu
In this work, a hybrid monolith incorporated with graphene oxide (GO) was prepared in the first time with binary green porogens of deep eutectic solvents (DESs) and room temperature ionic liquids (RTILs). GO was modified with 3-(trimethoxysilyl) propylmethacrylate (γ-MPS), and the resultant GO-MPS can be incorporated into poly (methacrylic acid-co-butylmethacrylate-co-ethylene glycol dimethacrylate) monoliths covalently. A hybrid monolithic column with high permeability and homogeneity can be achieved due to good dispersion of GO-MPS in the green solvents...
February 1, 2018: Talanta
Kangning Zhang, Xiaoling Chen, Zhuang Li, Yan Wang, Sen Sun, Li'na Wang, Tong Guo, Dongxia Zhang, Zhonghua Xue, Xibin Zhou, Xiaoquan Lu
In this work, a novel nanohybrid (AuPtNPs/S-NS-GR) of well-defined Au-Pt bimetallic nanoparticles (Au-PtNPs) decorated on sulfonated nitrogen sulfur co-doped graphene (S-NS-GR) was developed. Firstly, nitrogen sulfur co-doped graphene (NS-GR) was synthesized by one-step thermal annealing method. Secondly, phenyl SO3H- group was introduced onto the surface of NS-GR via diazotization reaction, which could provide more binding sites for the formation of metal nanoparticles. Finally, Au-Pt bimetallic nanoparticles were anchored on the surface of S-NS-GR by using electrochemical deposition...
February 1, 2018: Talanta
Marina Baccarin, Priscila Cervini, Eder Tadeu Gomes Cavalheiro
A bare composite graphite-polyurethane electrode (EGPU) and two other modified with graphene (EGPU-GR) and functionalized multi-walled carbon nanotubes (EGPU-CNTs) were prepared and compared regarding their voltammetric response to escitalopran (EST). The modifiers were characterized by Raman spectroscopy and the resulting electrode materials by contact angle measurements with a hydrophilicity character in the ascending order for the composites: GPU > GPU-GR > GPU-CNTs and scanning electron microscopy (SEM)...
February 1, 2018: Talanta
Shengxia Duan, Xuetao Xu, Xia Liu, Yanan Wang, Tasawar Hayat, Ahmed Alsaedi, Yuedong Meng, Jiaxing Li
In this study, magnetic Fe3O4 nanoparticles (MNP) modified with O-phosphorylethanolamine (O-PEA) were successfully prepared by non-thermal plasma induced method with different treatment times. The raw and modified MNP were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared spectrophotometer (FT-IR), and vibration sample magnetometer (VSM). The modified MNP samples show higher adsorption capacity for U(VI) removal and longer treatment time results in higher adsorption capacity and efficiency...
November 3, 2017: Journal of Colloid and Interface Science
Amirhassan Amiri, Ferial Ghaemi
In this study, graphene grown on stainless steel mesh efficiently incorporates the advanced properties of graphene in the form of an ultra-thin coating with the open geometry of the substrate, resulting in a highly sensitive and fast sorptive device capable of extracting target analytes directly from sample matrices. The synthesis of graphene on the stainless steel mesh was confirmed by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The advantages of the new microextraction device have been investigated for the determination of polycyclic aromatic hydrocarbons (PAHs) in environmental water samples in combination with gas chromatography-flame ionization detector (GC-FID)...
November 22, 2017: Analytica Chimica Acta
Zeynep Talip, Rugard Dressler, Jean-Christophe David, Christof Vockenhuber, Elisabeth Müller Gubler, Alexander Voegele, Erik Strub, Peter Vontobel, Dorothea Schumann
In this study, distillation, precipitation, and ion exchange methods were choosen for the separation of the long-lived β-emitters (129)I , (36)Cl and the α-emitters (154)Dy, (148)Gd, (150)Gd and (146)Sm from Ta targets irradiated with protons up to 2.6 GeV to determine their production cross sections. Measurements of (129)I/(127)I and (36)Cl/Cl ratios were performed with accelerator mass spectrometry (AMS). After separation of the lanthanides, the molecular plating technique was applied to prepare thin samples to obtain high-resolved α-spectra...
November 9, 2017: Analytical Chemistry
Yuan Cheng, Kangning Sun
Yb(3+),Er(3+),Eu(3+) triply-doped in YVO4 with varying molar ratio of Er/Eu were synthesized in a sol-gel method with a subsequent heat treatment for the first time. The optimal molar ratio of Er/Eu for the maximum energy transfer efficiency was 1/39. After molar ratio, the influences of different heat treatment temperatures were also researched and the maximal heat temperature was 1300 °C. Besides, the properties of YVO4:Yb(3+),Er(3+),Eu(3+) upconversion phosphors were investigated by X-ray diffraction, scanning electron microscopy and photo luminescent spectra, respectively...
November 6, 2017: Journal of Fluorescence
Omid Rezvani, Mahin Hashemi Hedeshi, Habib Bagheri
In this study, a low-cost microfluidic device from polymethyl methacrylate was fabricated by laser engraving technique. The device is consisted of a central chip unit with an aligned microchannel. Both sides of the engraved microchannel were sandwiched by two synthesized sheets from polyamide/titania (PA/TiO2) hollow nanofibers as extractive phases. The inlet and outlet of the device were connected to the polyether ether ketone tubes, while a peristaltic pump was used to deliver both sample and desorbing solvent through the microchannel...
October 25, 2017: Journal of Chromatography. A
Hamed Nosrati, Marziyeh Salehiabar, Hamidreza Kheiri Manjili, Hossein Danafar, Soodabeh Davaran
In this study, iron oxide magnetic bovine serum albumin core-shell nanoparticles (BSA coated IONPs) with narrow particle size distribution were synthesized under one-pot reaction via the desolvation and chemical co-precipitation method. Functionalized IONPs were characterized by X-ray diffraction (XRD), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), fourier transform infrared spectroscopy (FTIR), and transmission electron microscopy (TEM) techniques. Furthermore, vibrating sample magnetometer (VSM) analysis show these nanoparticles (NPs) have an excellent magnetic properties...
October 31, 2017: International Journal of Biological Macromolecules
Zufei Feng, Yan Lu, Yingjuan Zhao, Helin Ye
On the basis of magnetic molecularly imprinted polymer (MMIP) solid-phase extraction coupled with high performance liquid chromatography, we established a new method for the determination of the 4-methylimidazole (4-MEI) in soy sauce. Scanning electron microscopy (SEM), Fourier transform infrared (FT-IR), X-ray diffraction (XRD) and vibrating sample magnetometer (VSM) were used to characterize the synthesized MMIPs. To evaluate the polymers, batch rebinding experiments were carried out. The binding strength and capacity were determined from the derived Freundlich isotherm (FI) equation...
November 2, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
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