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Sample preparation for scanning electron microscopy

Peter Bond, David B Parkinson
The use of electron microscopy allows analysis of the microarchitecture of peripheral nerves both during development and in the processes of nerve regeneration and repair.We describe a novel method for the rapid analysis and quantification of myelin in peripheral nerve using a low vacuum scanning electron microscopy protocol. For this methodology, excised nerves are prepared for traditional transmission electron microscopy (TEM) imaging, but at the stage where semi-thin sections would be taken, the resin block is instead imaged at low vacuum in the scanning electron microscope (SEM) using the backscattered electron signal...
2018: Methods in Molecular Biology
Wei Du, Bilin Zhang, Pengqi Guo, Guoning Chen, Chun Chang, Qiang Fu
Dexamethasone-imprinted polymers were fabricated by reversible addition fragmentation chain transfer polymerization on the surface of magnetic nanoparticles under mild polymerization conditions, which exhibited a narrow polydispersity and high selectivity for dexamethasone extraction. The dexamethasone-imprinted polymers were characterized by scanning electron microscopy, transmission electron microscope, Fourier transform infrared spectroscopy, X-ray diffraction, energy dispersive spectrometry and vibrating sample magnetometry...
March 15, 2018: Journal of Separation Science
Truong Thi Tuong Vi, Selvaraj Rajesh Kumar, Bishakh Rout, Chi-Hsien Liu, Chak-Bor Wong, Chia-Wei Chang, Chien-Hao Chen, Dave W Chen, Shingjiang Jessie Lue
In this work, silver nanoparticles (Ag NPs) were decorated on thiol (-SH) grafted graphene oxide (GO) layers to investigate the antibacterial activities in Gram-positive bacteria ( Staphylococcus aureus ) and Gram-negative bacteria ( Pseudomonas aeruginosa ). The quasi-spherical, nano-sized Ag NPs were attached to the GO surface layers, as confirmed by using field emission scanning electron microscopy (FESEM) and transmission electron microscopy (TEM), respectively. The average size of GO-Ag nanocomposites was significantly reduced (327 nm) from those of pristine GO (962 nm) while the average size of loaded Ag NPs was significantly smaller than the Ag NPs without GO...
March 14, 2018: Nanomaterials
Selma Sassi, Christophe Candolfi, Christine Gendarme, Anne Dauscher, Bertrand Lenoir
The crystal structure and transport properties (2-723 K) of the homologous compound Pb5 Bi6 Se14 with partial substitution of Te for Se are studied by means of powder X-ray diffraction, scanning electron microscopy, electrical resistivity, thermopower, thermal conductivity and Hall effect measurements. Polycrystalline samples of Pb5 Bi6 Se14-x Tex (0 ≤ x ≤ 1.0) were prepared by a two-step synthesis method based on the pseudo-binary PbSe-Bi2 Se3 phase diagram combined with Te substitution in the PbSe precursor...
March 14, 2018: Dalton Transactions: An International Journal of Inorganic Chemistry
Yuejin Li, Xili Shang, Changhai Li, Xiaoming Huang, Jingjing Zheng
Novel visible-light-induced UiO-66/BiOI photocatalysts with a p-n junction structure have been prepared for the first time through a facile hydrothermal method. The prepared photocatalysts were characterized using the powder X-ray diffraction, high resolution transmission electron microscopy, scanning electron microscopy, UV-visible diffuse reflectance spectra, and N2 adsorption-desorption (Brunauer-Emmett-Teller) techniques respectively. The photodegradation performances of UiO-66/BiOI photocatalysts were evaluated by photodegrading salicylic acid under visible-light irradiation...
March 2018: Water Science and Technology: a Journal of the International Association on Water Pollution Research
Elisa Boanini, Paola Torricelli, Felix Sima, Emanuel Axente, Milena Fini, Ion N Mihailescu, Adriana Bigi
The chemistry, structure and morphology of the implant surface have a great influence on the integration of an implant material with bone tissue. In this work, we applied Combinatorial Matrix-Assisted Pulsed Laser Evaporation (C-MAPLE) to deposit gradient thin films with variable compositions of Sr-substituted hydroxyapatite (SrHA) and Zn-substituted β-tricalcium phosphate (ZnTCP) on Titanium substrates. Five samples with different SrHA/ZnTCP composition ratios were fabricated by a single step laser procedure...
March 6, 2018: Journal of Inorganic Biochemistry
Jin-Jin Chen, Peng-Fei Gong, Yi-Lan Liu, Bo-Yan Liu, Dawn Eggert, Yuan-Heng Guo, Ming-Xia Zhao, Qing-Sheng Zhao, Bing Zhao
A novel technique of ultrasound-assisted freeze-thaw pretreatment (UFP) was developed to improve the drying efficiency of maca and bioactive amide synthesis in maca. The optimal UFP conditions are ultrasonic processing 90 min at 30 °C with 6 freeze-thaw cycles. Samples with freeze-thaw pretreatment (FP), ultrasound pretreatment (UP), and UFP were prepared for further comparative study. A no pretreatment (NP) sample was included as a control. The results showed that UFP improved the drying efficiency of maca slices, showing the highest effective moisture diffusivity (1...
March 10, 2018: Journal of Food Science
Nanqiang Shang, Qingguo Chen, Xinzhe Wei
The conductivity mismatch in the composite insulation of high voltage direct current (HVDC) cable accessories causes electric field distribution distortion and even insulation breakdown. Therefore, a liquid silicone rubber (LSR) filled with SiC nanoparticles is prepared for the insulation of cable accessories. The micro-morphology of the SiC/LSR nanocomposites is observed by scanning electron microscopy, and their trap parameters are characterized using thermal stimulated current (TSC) tests. Moreover, the dielectric properties of SiC/LSR nanocomposites with different SiC concentrations are tested...
March 8, 2018: Materials
Patrycja Parnicka, Paweł Mazierski, Tomasz Grzyb, Wojciech Lisowski, Ewa Kowalska, Bunsho Ohtani, Adriana Zaleska-Medynska, Joanna Nadolna
Nd-modified TiO2 photocatalysts have been obtained via hydrothermal (HT) and sol-hydrothermal (SHT) methods. The as-prepared samples were characterized by X-ray diffraction (XRD), BET surface area measurements, scanning electron microscopy (SEM), diffuse reflectance spectroscopy (DRS), luminescence spectroscopy and X-ray photoelectron spectroscopy (XPS). The photocatalytic activity of the synthesized samples was evaluated by the degradation of phenol in aqueous solution under irradiation with UV-vis (λ > 350 nm) and vis (λ > 420 nm) light, as well as by the degradation of gaseous toluene under irradiation with vis (λmax = 415 nm) light...
2018: Beilstein Journal of Nanotechnology
Gulcin Bolat, Serdar Abaci
Malathion (MLT) is an organophosphorous type pesticide and having seriously high toxicity and electrochemical platforms for rapid, simple, inexpensive and sensitive determination of pesticides is still a special concern. This paper describes a simple preparation of a composite film consisting of ionic liquid (IL), chitosan (CS) and electrochemically synthesized gold nanoparticles (AuNPs) on single use pencil graphite electrodes (PGEs). The microscopic and electrochemical characterization of AuNP-CS-IL/PGE was studied using scanning electron microscopy, cyclic voltammetry and electrochemical impedance spectroscopy...
March 4, 2018: Sensors
Jeevitaa Kshersagar, Ravi Kshirsagar, Shashikant Desai, Raghvendra Bohara, Meghnad Joshi
Direct application of amnion has greater risk of immunological rejection and infection. Decellularization is an effective method to lower the risk of immune complications and infections. The bioreactor assembly with multiple cassettes was designed for decellurization of multiple amnions with different cell types simultaneously in single run. A detergent-based protocol was modified to remove all cellular components from amnion and diminish the DNA content to render it non-immunogenic. Amnion (n = 10) were treated with 2% sodium dodecyl sulphate (SDS), 5% dimethyl sulfoxide (DMSO) and 2% sodium deoxycholeate (SD)...
March 5, 2018: Cell and Tissue Banking
Hazuki Yonashiro, Kenjirou Higashi, Chikako Morikawa, Keisuke Ueda, Tsutomu Itoh, Masataka Ito, Hyuma Masu, Shuji Noguchi, Kunikazu Moribe
The morphology and stability of amorphous nanoparticles of glibenclamide (GLB) prepared by the antisolvent method using different methods of adding hypromellose (HPMC) were evaluated. Nano-A was prepared by the injection of a dimethyl sulfoxide (DMSO) solution of GLB into the HPMC solution, whereas nano-B was obtained by the injection of a DMSO solution of GLB and HPMC into water. Cryogenic transmission electron microscopy, field-emission scanning electron microscopy, and field-emission transmission electron microscopy including energy dispersive X-ray spectrometry revealed that the particles of the nano-A and nano-B samples are hollow spheres and non-spherical nanoparticles, respectively...
March 5, 2018: Molecular Pharmaceutics
Feng Luan, Shuang Zhang, Dandan Chen, Kun Zheng, Xuming Zhuang
Cobalt disulfide-decorated ionic liquid-functionalized graphene nanocomposites were prepared herein and characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), and X-ray photoelectron spectroscopy (XPS). The as-prepared nanocomposites were subsequently used to build a modified glassy carbon electrode serving as a hydrazine (N2 H4 ) electrochemical sensor. The electrocatalytic performance of the prepared sensor towards the N2 H4 oxidation reaction was evaluated by cyclic voltammetry (CV) and amperometric methods...
May 15, 2018: Talanta
Beatriz Fresco-Cala, Soledad Cárdenas
This article presents a monolithic extraction unit with integrated stirring using carbon nanohorns and methacrylate-based compounds as monomers. The hybrid monolithic material was prepared by thermal polymerization at 70 °C for 24 h, and was applied for the extraction of UV-filters from waters and human urine samples. To achieve the integrated stirring unit, the monolith was grown over an ironware. Variables dealing with the polymerization mixture composition and the microextraction procedure were studied in depth...
May 15, 2018: Talanta
Hongsheng Huang, Xiaoqin Feng, Tianbao Chen, Ying Xiong, Feng Zhang
Novel Ca2 B2 O5 ·H2 O:Eu3+ nanotubes, constructed with nanobelts, were prepared using a hydrothermal method. The Ca3 (BO3 )2 :Eu3+ nanobelts with a thickness of about 100 nm were made for the first time using a two-step hydrothermal process with Ca2 B2 O5 ·H2 O:Eu3+ as the precursor. The samples were characterized by energy dispersive X-ray spectroscopy, X-ray diffraction, Fourier transform infra-red spectroscopy, thermogravimetry differential thermal analysis and scanning electron microscopy. The relationship between Ca3 (BO3 )2 :Eu3+ and Ca2 B2 O5 ·H2 O:Eu3+ was also studied...
March 2, 2018: Luminescence: the Journal of Biological and Chemical Luminescence
Yuxiao Gong, Yan Wang, Guang Sun, Tiekun Jia, Lei Jia, Fengmei Zhang, Long Lin, Baoqing Zhang, Jianliang Cao, Zhanying Zhang
Recently, semiconducting metal oxide (SMO) gas sensors have attracted the attention of researchers for high conductivity, labile features by environment, low cost, easy preparation, etc. However, traditional SMOs have some defects such as higher operating temperature and lower response value, which greatly limit their application in the field of gas sensor. In this work, the carbon nitride decorated ball-flower like Co₃O₄ composite was successfully synthesized via a facile hydrothermal method, the composition and morphology of the as-synthesized samples were studied by the techniques of X-ray powder diffraction (XRD), Field-emission scanning electron microscopy (FESEM), Transmission electron microscopy (TEM), Fourier transform infrared spectrometer (FT-IR) and N₂-sorption...
February 27, 2018: Nanomaterials
Ruining Li, Zhaowei Wang, Jialei Guo, Yan Li, Hanyu Zhang, Junmin Zhu, Xiaoyun Xie
Potato stems and leaves biochar (PB) was prepared by pyrolysis at a temperature of 500°C under anoxic conditions. In order to strengthen the adsorption capacity, biochar was modified with alkaline solution (alkali modified biochar, APB). Two kinds of biochars were adopted as adsorbents to remove ciprofloxacin (CIP) from aqueous solution. The adsorption behavior of CIP onto biochar before and after alkali modified including adsorption kinetics and isotherms were investigated. The effects of different factors (equilibrium time, pH, temperature and initial concentration) during the adsorption process were also investigated...
February 2018: Water Science and Technology: a Journal of the International Association on Water Pollution Research
Wieslaw J Roth, Barbara Gil, Alvaro Mayoral, Justyna Grzybek, Aleksandra Korzeniowska, Martin Kubu, Wacław Makowski, Jiří Čejka, Zbigniew Olejniczak, Michal Mazur
Layered zeolite materials with FER layer topology can produce various condensed and expanded structures including zeolite frameworks, FER and CDO, their interlayer expanded forms (IEZ), and organic-intercalated and pillared derivatives. This work concerns pillaring of the surfactant-swollen derivative with a gallery height of ca. 2.5 nm between layers by treatment with tetraethylorthosilicate (TEOS) at room and elevated temperatures. The materials obtained at 100 °C and higher showed unusual properties including 2 nm pores on the micro/mesoporous border and disordered layer packing indicated by the absence of distinct low angle interlayer peaks at d-spacing >3 nm (∼3° 2θ Cu Kα radiation) in the X-ray diffraction pattern (XRD)...
February 27, 2018: Dalton Transactions: An International Journal of Inorganic Chemistry
Ali Rayegan, Alireza Allafchian, Ismaeil Abdolhosseini, Parviz Kameli
A novel drug delivery system, loaded the drug cephalexin on the basil seed mucilage coated magnetic nanoparticles (Fe3 O4 @BSM-CPX) was prepared and characterized by means of X-ray diffraction (XRD), Furier Transform Infrared (FTIR), Field Emission Scanning Electron Microscope (FESEM), Vibrating Sample Magnetometer (VSM), Transmission Electron Microscopy (TEM), and Anti-bacterial, and Specific Surface (BET). By comparing the size of the uncoated nanoparticles (12 nm) and the size of the coated magnetite nanoparticles (6 nm), it was found that with the mucilage coating being put on the magnetite nanoparticles, the size of the nanoparticle cores has also decreased...
February 23, 2018: International Journal of Biological Macromolecules
Xian-Peng Zhang, Jian Luo, Tong-Fang Jing, Da-Xia Zhang, Bei-Xing Li, Feng Liu
Microcapsules (MCs) prepared with diverse wall material structures may exhibit different properties. In this study, MCs were fabricated with three kinds of epoxy phenolic novolac resins (EPNs), which possessed unique epoxy values as wall-forming materials by interfacial polymerization. The effects of the EPN types on the surface morphology, particle size distribution, encapsulation efficiency, thermal stability as well as release behavior and bioactivity of the MCs were investigated. In all three samples, the MCs had nearly spherical shapes with fine monodispersities and sizes in the range of 7-30 μm...
February 13, 2018: Colloids and Surfaces. B, Biointerfaces
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