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https://www.readbyqxmd.com/read/28098963/scaffolding-of-enzymes-on-virus-nanoarrays-effects-of-confinement-and-virus-organization-on-biocatalysis
#1
Anisha N Patel, Agnès Anne, Arnaud Chovin, Christophe Demaille, Eric Grelet, Thierry Michon, Cécilia Taofifenua
Organizing active enzyme molecules on nanometer-sized scaffolds is a promising strategy for designing highly efficient supported catalytic systems for biosynthetic and sensing applications. This is achieved by designing model nanoscale enzymatic platforms followed by thorough analysis of the catalytic activity. Herein, the virus fd bacteriophage is considered as an enzyme nanocarrier to study the scaffolding effects on enzymatic activity. Nanoarrays of randomly oriented, or directionally patterned, fd bacteriophage virus are functionalized with the enzyme glucose oxidase (GOx), using an immunological assembly strategy, directly on a gold electrode support...
January 18, 2017: Small
https://www.readbyqxmd.com/read/28098889/isomir-expression-patterns-in-canonical-and-dicer%C3%A2-independent-micrornas
#2
Tingming Liang, Jiafeng Yu, Chang Liu, Li Guo
Multiple microRNA (miRNA) variants, known as isomiRs, are extensively distributed in miRNA loci and predominantly derive from the alternative cleavage of Drosha/Dicer and 3'addition events. The present study aimed to investigate the expression patterns of multiple isomiRs in typical miRNA and Dicer‑independent miRNA loci by conducting evolutionary and expression analysis using public datasets. Although different miRNA maturation processes exist, multiple isomiRs can be detected by similar expression distributions...
January 13, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28098883/developmental-expression-of-toll%C3%A2-like-receptors-in-the-guinea-pig-lung
#3
Lingjie Ma, Jiali Yang, Li Yang, Juan Shi, Jing Xue, Yong Li, Xiaoming Liu
The guinea pig is a useful model for investigating infectious and non‑infectious lung diseases due to the sensitivity of its respiratory system and susceptibility to infectious agents. Toll‑like receptors (TLRs) are important components of the innate immune response and are critical for lung immune function. In the present study, the differentiation of epithelial cells in the guinea pig lung was examined during gestation by studying anatomic morphology and the major epithelial cell types using cell type‑specific markers...
January 17, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28098841/compressed-sensing-techniques-applied-to-ultrasonic-imaging-of-cargo-containers
#4
Yuri Álvarez López, José Ángel Martínez Lorenzo
One of the key issues in the fight against the smuggling of goods has been the development of scanners for cargo inspection. X-ray-based radiographic system scanners are the most developed sensing modality. However, they are costly and use bulky sources that emit hazardous, ionizing radiation. Aiming to improve the probability of threat detection, an ultrasonic-based technique, capable of detecting the footprint of metallic containers or compartments concealed within the metallic structure of the inspected cargo, has been proposed...
January 15, 2017: Sensors
https://www.readbyqxmd.com/read/28098822/application-of-fault-tree-analysis-and-fuzzy-neural-networks-to-fault-diagnosis-in-the-internet-of-things-iot-for-aquaculture
#5
Yingyi Chen, Zhumi Zhen, Huihui Yu, Jing Xu
In the Internet of Things (IoT) equipment used for aquaculture is often deployed in outdoor ponds located in remote areas. Faults occur frequently in these tough environments and the staff generally lack professional knowledge and pay a low degree of attention in these areas. Once faults happen, expert personnel must carry out maintenance outdoors. Therefore, this study presents an intelligent method for fault diagnosis based on fault tree analysis and a fuzzy neural network. In the proposed method, first, the fault tree presents a logic structure of fault symptoms and faults...
January 14, 2017: Sensors
https://www.readbyqxmd.com/read/28098806/antibacterial-activities-and-possible-modes-of-action-of-acacia-nilotica-l-del-against-multidrug-resistant-escherichia-coli-and-salmonella
#6
Muhammad Bilal Sadiq, Joel Tarning, Tay Zar Aye Cho, Anil Kumar Anal
Medicinal plants are frequently used for the treatment of various infectious diseases. The objective of this study was to evaluate the antibacterial activity and mode of action of Acacia nilotica and the antibiogram patterns of foodborne and clinical strains of Escherichia coli and Salmonella. The mechanism of action of acacia extracts against E. coli and Salmonella was elucidated by observing morphological damages including cell integrity and cell membrane permeability, as well as changes in cell structures and growth patterns in kill-time experiments...
January 14, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28098734/multimodal-fundus-imaging-of-outer-retinal-tubulations-in-choroidal-osteoma-patients
#7
Yi Xuan, Yongjin Zhang, Min Wang, Jinli Guo, Lei Li, Wei Liu, Xiaofeng Ye
PURPOSE: To evaluate the morphological findings of outer retinal tubulations (ORTs) shown on multimodal imaging modalities in patients with choroidal osteoma. METHODS: Nineteen eyes of 17 patients with choroidal osteoma underwent full clinical and imaging assessments. Color fundus photography, spectral-domain optical coherence tomography (OCT), and en face OCT were used to identify and detect the characteristics of ORT structures, including the shape, configuration, location, and distribution in the fundus...
January 16, 2017: Retina
https://www.readbyqxmd.com/read/28098723/assessment-of-the-internal-limiting-membrane-status-when-a-macular-epiretinal-membrane-is-removed-in-a-prospective-study
#8
Daniel Vila Solà, Cristofer Nienow, Ignasi Jürgens
PURPOSE: To describe the presence and integrity of the internal limiting membrane (ILM) after removal of the macular epiretinal membrane (ERM) and to investigate the accuracy of tissue identification using surgical dyes when compared to histopathology results. METHODS: Patients with idiopathic ERM were enrolled in a prospective study and randomized to one of two surgical techniques. In one (Group M), only the ERM was deliberately removed and in the other (Group L) the ILM was also removed...
January 16, 2017: Retina
https://www.readbyqxmd.com/read/28098588/choice-of-anesthesia-for-cesarean-delivery-an-analysis-of-the-national-anesthesia-clinical-outcomes-registry
#9
Jeremy Juang, Rodney A Gabriel, Richard P Dutton, Arvind Palanisamy, Richard D Urman
Neuraxial anesthesia use in cesarean deliveries (CDs) has been rising since the 1980s, whereas general anesthesia (GA) use has been declining. In this brief report we analyzed recent obstetric anesthesia practice patterns using National Anesthesiology Clinical Outcomes Registry data. Approximately 218,285 CD cases were identified between 2010 and 2015. GA was used in 5.8% of all CDs and 14.6% of emergent CDs. Higher rates of GA use were observed in CDs performed in university hospitals, after hours and on weekends, and on patients who were American Society of Anesthesiologists class III or higher and 18 years of age or younger...
January 16, 2017: Anesthesia and Analgesia
https://www.readbyqxmd.com/read/28098400/role-of-contrast-enhanced-sonography-in-the-evaluation-of-axillary-lymph-nodes-in-breast-carcinoma-a-monocentric-study
#10
Giacomo Agliata, Gianluca Valeri, Giulio Argalia, Elisa Tarabelli, Gian Marco Giuseppetti
OBJECTIVES: To evaluate the diagnostic performance of contrast-enhanced sonography for characterization of the lymph node status (metastatic or not) in patients with breast carcinomas by comparison with sentinel lymph node biopsy. METHODS: From January to August 2015, 50 female patients with a histologic diagnosis of invasive breast carcinoma were prospectively examined by ipsilateral axillary contrast-enhanced sonography. The test was performed by a single radiologist using an ultrasound system with a broadband 8-12-MHz, 38-mm high-resolution linear transducer...
January 18, 2017: Journal of Ultrasound in Medicine: Official Journal of the American Institute of Ultrasound in Medicine
https://www.readbyqxmd.com/read/28098195/global-mapping-of-nonseismic-sea-level-oscillations-at-tsunami-timescales
#11
Ivica Vilibić, Jadranka Šepić
Present investigations of sea level extremes are based on hourly data measured at coastal tide gauges. The use of hourly data restricts existing global and regional analyses to periods larger than 2 h. However, a number of processes occur at minute timescales, of which the most ruinous are tsunamis. Meteotsunamis, hazardous nonseismic waves that occur at tsunami timescales over limited regions, may also locally dominate sea level extremes. Here, we show that nonseismic sea level oscillations at tsunami timescales (<2 h) may substantially contribute to global sea level extremes, up to 50% in low-tidal basins...
January 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28098073/classical-density-functional-study-of-wetting-transitions-on-nanopatterned-surfaces
#12
P Yatsyshin, A O Parry, C Rascón, S Kalliadasis
Even simple fluids on simple substrates can exhibit very rich surface phase behaviour. To illustrate this, we consider fluid adsorption on a planar wall chemically patterned with a deep stripe of a different material. In this system, two phase transitions compete: unbending and pre-wetting. Using microscopic density-functional theory, we show that, for thin stripes, the lines of these two phase transitions may merge, leading to a new two-dimensional-like wetting transition occurring along the walls. The influence of intermolecular forces and interfacial fluctuations on this phase transition and at complete pre-wetting are considered in detail...
January 18, 2017: Journal of Physics. Condensed Matter: An Institute of Physics Journal
https://www.readbyqxmd.com/read/28098049/influence-of-barley-grain-particle-size-and-treatment-with-citric-acid-on-digestibility-ruminal-fermentation-and-microbial-protein-synthesis-in-holstein-calves
#13
M Kazemi-Bonchenari, A Z M Salem, S López
Chemical and physical treatments of barley grain increase ruminally resistant starch and can improve the rumen fermentation pattern. The objective of the present study was to evaluate the effects of chemical (addition of citric acid, CA) and physical (grinding to two different particle sizes, PS) treatment of barley grain on performance, rumen fermentation, microbial protein yield in the rumen and selected blood metabolites in growing calves. In all, 28 male Holstein calves (172±5.1 kg initial BW) were used in a complete randomised design with a factorial arrangement of 2 barley grain particle sizes×2 levels of citric acid...
January 18, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/28097869/a-solvent-free-processable-and-photo-patternable-hybrid-gate-dielectric-for-flexible-top-gate-organic-field-effect-transistors
#14
Jun Seon Kwon, Han-Wool Park, Do Hwan Kim, Young-Je Kwark
We report a novel solvent-free and direct photo-patternable poly[(mercaptopropyl)methyl-siloxane] (PMMS) hybrid dielectric for flexible top-gate organic field-effect transistors (OFETs), utilizing a photo-activated thiol-ene reaction under UV irradiation of 254 nm to induce cross-linking, even in air and at low temperature. In particular, a solvent-free PMMS-f dielectric film, for which an optimal cross-linking density is shown by a well-organized molar ratio between thiol and vinyl in the thiol-ene reaction, exhibited a high dielectric constant (5...
January 18, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28097826/development-shapes-a-consistent-inbreeding-effect-in-mouse-crania-of-different-line-crosses
#15
Mihaela Pavličev, Philipp Mitteroecker, Paula M Gonzalez, Campbell Rolian, Heather Jamniczky, Fernando Pardo-Manuel Villena, Ralph Marcucio, Richard Spritz, Benedikt Hallgrimsson
Development translates genetic variation into a multivariate pattern of phenotypic variation, distributing it among traits in a nonuniform manner. As developmental processes are largely shared within species, this suggests that heritable phenotypic variation will be patterned similarly, in spite of the different segregating alleles. To investigate developmental effect on the variational pattern in the shape of the mouse skull across genetically differentiated lines, we employed the full set of reciprocal crosses (a...
January 18, 2017: Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution
https://www.readbyqxmd.com/read/28097699/maternal-red-blood-cell-alloantibodies-identified-in-blood-samples-obtained-from-iranian-pregnant-women-the-first-population-study-in-iran
#16
Ehsan Shahverdi, Mostafa Moghaddam, Fateme Gorzin
BACKGROUND: The objective was to determine the frequency of occurrence of alloantibodies among pregnant women in Iran. STUDY DESIGN AND METHODS: This was a prospective cross-sectional study, which was carried out in the immunohematology reference laboratory of the Iranian Blood Transfusion Organization in Tehran, Iran, in 2008 to 2015. Screening and identification of red blood cell (RBC) alloantibodies was done on the sera of 7340 pregnant females using the standard tube method and gel column agglutination technique...
January 2017: Transfusion
https://www.readbyqxmd.com/read/28097690/an-overview-of-structural-features-of-antibacterial-glycoconjugate-vaccines-that-influence-their-immunogenicity
#17
REVIEW
Farjana Khatun, Rachel J Stephenson, Istvan Toth
Bacterial cell-surface-derived or mimicked carbohydrate moieties that act as protective antigens are used in the development of antibacterial glycoconjugate vaccines. The carbohydrate antigen must have a minimum length or size to maintain the conformational structure of the antigenic epitope(s). The presence or absence of O-acetate, phosphate, glycerol phosphate and pyruvate ketal plays a vital role in defining the immunogenicity of the carbohydrate antigen. The nature of the carrier protein, spacer and conjugation pattern used to develop the glycoconjugate vaccine also defines its overall spatial orientation which in turn affects its avidity and selectivity of interaction with the desired target(s)...
November 7, 2016: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28097675/the-swamplands-of-reflection-using-conversation-analysis-to-reveal-the-architecture-of-group-reflection-sessions
#18
Mario Veen, Anne de la Croix
CONTEXT: Many medical schools include group reflection in their curriculum, and many researchers have considered both the concept and the outcomes of reflection. However, no research has been carried out on how 'reflective talk' is structured in the classroom. This paper describes how tutors and residents organise group reflection sessions in situ by describing an example of group reflection in medical education. Our aim is to provide an evidence base that can be used by medical educators to think about the way reflection should be included in their curriculum...
January 18, 2017: Medical Education
https://www.readbyqxmd.com/read/28097621/practice-patterns-of-venous-thromboembolism-prophylaxis-in-underweight-critically-ill-patients-with-neurologic-injury
#19
Kevin Betthauser, Hannah Pope, Mollie Gowan, Theresa Human
PURPOSE: Venous thromboembolism (VTE) prophylaxis in underweight patients with neurologic injury remains unaddressed by guidelines and primary literature. This study aimed to describe VTE prophylaxis strategies employed in this population and compare the impact of underweight and non-obese patients on thrombotic and bleeding events. METHODS: A retrospective review of adults admitted with a diagnosis of neurologic injury to a neurology/neurosurgery intensive care unit (ICU) over 6 years...
January 17, 2017: Neurocritical Care
https://www.readbyqxmd.com/read/28097597/self-powered-active-sensor-with-concentric-topography-of-piezoelectric-fibers
#20
Yiin Kuen Fuh, Zih Ming Huang, Bo Sheng Wang, Shan Chien Li
In this study, we demonstrated a flexible and self-powered sensor based on piezoelectric fibers in the diameter range of nano- and micro-scales. Our work is distinctively different from previous electrospinning research; we fabricated this apparatus precisely via near-field electrospinning which has a spectacular performance to harvest mechanical deformation in arbitrary direction and a novel concentrically circular topography. There are many piezoelectric devices based on electrospinning polymeric fibers. However, the fibers were mostly patterned in parallel lines and they could be actuated in limited direction only...
December 2017: Nanoscale Research Letters
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