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https://www.readbyqxmd.com/read/28647575/a-modified-technique-of-temporary-suspension-of-the-ovary-to-the-anterior-abdominal-wall
#1
Omar M Abuzeid, Rubin Raju, John Hebert, Mohammad Ashraf, Mostafa I Abuzeid
STUDY OBJECTIVE: A previous study described a technique of temporary suspension of the ovary to the abdominal wall using nylon suture (Abuzeid et al 2002). The aim of this study is to describe a modified technique of temporary suspension of the ovary to the fascia of the anterior abdominal wall after operative laparoscopy for advanced stage endometriosis to reduce postoperative adhesion formation. DESIGN: Video illustrating this modified technique of ovarian suspension SETTING: Academic affiliated community medical center PATIENTS: This patient underwent temporary suspension of the right ovary, using 3-0 plain catgut suture, after operative laparoscopy for advanced stage endometriosis (Stage III ASRM classification)...
June 21, 2017: Journal of Minimally Invasive Gynecology
https://www.readbyqxmd.com/read/28643456/irf6-expression-in-basal-epithelium-partially-rescues-irf6-knockout-mice
#2
Youssef A Kousa, Dina Moussa, Brian C Schutte
BACKGROUND: Mutations in IRF6, CHUK (IKKA) and RIPK4 can lead to a disease spectrum that includes cutaneous, limb and craniofacial malformations. Loss of these alleles in the mouse leads to perinatal lethality and severe cutaneous, limb and craniofacial defects. Genetic rescue in the mouse has been shown for Ikka and Ripk4. RESULTS: Here, we show partial genetic rescue of Irf6 knockout embryos using the KRT14 promoter to drive Irf6 expression in the basal epithelium...
June 23, 2017: Developmental Dynamics: An Official Publication of the American Association of Anatomists
https://www.readbyqxmd.com/read/28642872/chronic-expanding-hematoma-in-the-extremities-a-clinical-problem-of-adhesion-to-the-surrounding-tissues
#3
Akio Sakamoto, Takeshi Okamoto, Shuichi Matsuda
Chronic expanding hematoma is characterized by continuous growth of a blood collection. We analyzed the clinical features of 7 patients with chronic expanding hematomas in the extremities, with an average age of 65.6 years. All lesions occurred in the lower extremities, with 4 seen in the thigh and 3 in the knee region. Six patients had subcutaneous hematomas, while 1 was deep-seated in the thigh. The magnetic resonance features of the lesion were compatible with those of a standard hematoma. A low signal intensity on T1- and T2-weighted imaging at the pseudocapsule was also characteristic...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28639563/the-role-of-apical-contractility-in-determining-cell-morphology-in-multilayered-epithelial-sheets-and-tubes
#4
Rui Zhen Tan, Tanny Lai, Keng-Hwee Chiam
A multilayered epithelium is made up of individual cells that are stratified in an orderly fashion, layer by layer. In such tissues, individual cells can adopt a wide range of shapes ranging from columnar to squamous. From histological images, we observe that, in flat epithelia such as the skin, the cells in the top layer are squamous while those in the middle and bottom layers are columnar, whereas in tubular epithelia, the cells in all layers are columnar. We develop a computational model to understand how individual cell shape is governed by the mechanical forces within multilayered flat and curved epithelia...
June 22, 2017: Physical Biology
https://www.readbyqxmd.com/read/28636748/e-cadherin-mediates-uvr-and-calcium-induced-melanin-transfer-in-human-skin-cells
#5
Suman K Singh, Richard Baker, Stephen K Sikkink, Carine Nizard, Sylvianne Schnebert, Robin Kurfurst, Desmond J Tobin
Skin pigmentation is directed by epidermal-melanin units, characterized by long-lived and dendritic epidermal melanocytes (MC) that interact with viable keratinocytes (KC) to contribute melanin to the epidermis. Previously we reported that MC:KC contact is required for melanosome transfer, that this can be enhanced by filopodial and by UVR/UVA irradiation, which can up-regulate melanosome transfer via Myosin X-mediated control of MC filopodia. Both MC and KC express Ca(2+) -dependent E-cadherins. These homophilic adhesion contacts induce transient increases in intra-KC Ca(2+) , while ultraviolet radiation (UVR) raises intra-MC Ca(2+) via calcium selective ORAI1 ion channels; both are associated with regulating melanogenesis...
June 21, 2017: Experimental Dermatology
https://www.readbyqxmd.com/read/28629001/biomimetic-electrospun-scaffolds-from-main-extracellular-matrix-components-for-skin-tissue-engineering-application-the-role-of-chondroitin-sulfate-and-sulfated-hyaluronan
#6
Sirsendu Bhowmick, Sandra Rother, Heike Zimmermann, Poh S Lee, Stephanie Moeller, Matthias Schnabelrauch, Veena Koul, Rainer Jordan, Vera Hintze, Dieter Scharnweber
Incorporation of bioactive components like glycosaminoglycans (GAGs) into tissue engineering scaffolds, is a promising approach towards developing new generation functional biomaterial. Here, we have designed electrospun nanofibrous scaffolds made of gelatin and different concentrations of chemically sulfated or non-sulfated hyaluronan (sHA or HA) and chondroitin sulfate (CS). Evenly distributed fiber morphology was observed with no differences between varying concentrations and types of GAGs. In vitro release kinetics revealed that GAGs release is driven by diffusion...
October 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28627382/bpag1-a-distinctive-role-in-skin-and-neurological-diseases
#7
REVIEW
Arshad Ali, Lifang Hu, Fan Zhao, Wuxia Qiu, Pai Wang, Xiaoli Ma, Yan Zhang, Lei Chen, Airong Qian
Spectraplakins are multifunctional cytoskeletal linker proteins that act as important communicators, connecting cytoskeletal components with each other and to cellular junctions. Bullous pemphigoid antigen 1 (BPAG1)/dystonin is a member of spectraplakin family and expressed in various tissues. Alternative splicing of BPAG1 gene produces various isoforms with unique structure and domains. BPAG1 plays crucial roles in numerous biological processes, such as cytoskeleton organization, cell polarization, cell adhesion, and cell migration as well as signaling transduction...
June 13, 2017: Seminars in Cell & Developmental Biology
https://www.readbyqxmd.com/read/28623693/control-of-human-skin-wettability-using-the-ph-of-anionic-surfactant-solution-treatments
#8
L Bromberg, X Liu, I Wang, S Smith, K Schwicker, Z Eller, G K German
The outermost layer of human skin, or stratum corneum, acts as a protective barrier between underlying living tissue and the external environment. The wettability of this tissue layer can influence spreading of chemicals and the adhesion of pathogenic microorganisms. We show in this article that the wettability of isolated human stratum corneum can be controlled through treatment with solutions of the anionic surfactant, sodium lauryl sulfate, buffered to different pH values. Relative to control treatments with the buffer solution alone, surfactant solution treatments under acidic conditions cause delipidated stratum corneum to become more hydrophobic...
June 9, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28622369/comparative-sequence-analysis-reveals-regulation-of-genes-in-developing-schistosomula-of-schistosoma-mansoni-exposed-to-host-portal-serum
#9
Wander de Jesus Jeremias, Flávio Marcos Gomes Araújo, Fábio Ribeiro Queiroz, Fabiano Sviatopolk Mirsky Pais, Ana Carolina Alves de Mattos, Anna Christina de Matos Salim, Paulo Marcos Zech Coelho, Guilherme Correa Oliveira, John Robert Kusel, Renata Guerra-Sá, Roney Santos Coimbra, Élio Hideo Babá
Once inside a vertebrate host after infection, individual schistosomula of the parasite Schistosoma mansoni find a new and complex environment, which requires quick adjustments for survival, such as those that allow it to avoid the innate immune response of the host. Thus, it is very important for the parasite to remain within the skin after entering the host for a period of about 3 days, at which time it can then reach the venous system, migrate to the lungs and, by the end of eighth day post-infection, it reach the portal venous system, while undergoing minimal changes in morphology...
2017: PloS One
https://www.readbyqxmd.com/read/28617467/a-wet-tolerant-adhesive-patch-inspired-by-protuberances-in-suction-cups-of-octopi
#10
Sangyul Baik, Da Wan Kim, Youngjin Park, Tae-Jin Lee, Suk Ho Bhang, Changhyun Pang
Adhesion strategies that rely on mechanical interlocking or molecular attractions between surfaces can suffer when coming into contact with liquids. Thus far, artificial wet and dry adhesives have included hierarchical mushroom-shaped or porous structures that allow suction or capillarity, supramolecular structures comprising nanoparticles, and chemistry-based attractants that use various protein polyelectrolytes. However, it is challenging to develop adhesives that are simple to make and also perform well-and repeatedly-under both wet and dry conditions, while avoiding non-chemical contamination on the adhered surfaces...
June 14, 2017: Nature
https://www.readbyqxmd.com/read/28606546/advanced-multi-targeted-composite-biomaterial-dressing-for-pain-and-infection-control-in-chronic-leg-ulcers
#11
Ovidio Catanzano, Rachael Docking, Patricia Schofield, Joshua Boateng
This study aimed to develop advanced biomaterial polysaccharide based dressings to manage pain associated with infected chronic leg ulcers in older adults. Composite carrageenan (CARR) and hyaluronic acid (HA) dressings loaded with lidocaine (LID) and AgNPs were formulated as freeze-dried wafers and functionally characterized for porous microstructure (morphology), mechanical strength, moisture handling properties, swelling, adhesion and LID release. Antimicrobial activity of AgNPs was evaluated (turbidity assay) against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus whilst cell viability studies (MTT) was performed on normal adult human primary epidermal keratinocyte cells...
September 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28600798/inhibition-of-p38mapk-signaling-prevents-epidermal-blistering-and-alterations-of-desmosome-structure-induced-by-pemphigus-autoantibodies-in-human-epidermis
#12
D T Egu, E Walter, V Spindler, J Waschke
BACKGROUND: Pemphigus vulgaris (PV) is a skin blistering disease caused by autoantibodies targeting the desmosomal adhesion proteins desmoglein (Dsg) 3 and 1. The mechanisms underlying pemphigus skin blistering are not fully elucidated but p38MAPK activation is one of the signaling events necessary for full loss of cell cohesion. However, it is unclear whether ultrastructural hallmarks of desmosome morphology as observed in patients' lesions are mediated by p38MAPK signaling. OBJECTIVE: In this study, we tested the relevance of p38MAPK for blister formation and the ultrastructural changes induced by PV autoantibodies in human skin...
June 10, 2017: British Journal of Dermatology
https://www.readbyqxmd.com/read/28598978/an-evaluation-tool-for-myofascial-adhesions-in-patients-after-breast-cancer-map-bc-evaluation-tool-development-and-interrater-reliability
#13
An De Groef, Marijke Van Kampen, Nele Vervloesem, Sophie De Geyter, Evi Dieltjens, Marie-Rose Christiaens, Patrick Neven, Inge Geraerts, Nele Devoogdt
PURPOSE: To develop a tool to evaluate myofascial adhesions objectively in patients with breast cancer and to investigate its interrater reliability. METHODS: 1) Development of the evaluation tool. Literature was searched, experts in the field of myofascial therapy were consulted and pilot testing was performed. 2) Thirty patients (63% had a mastectomy, 37% breast-conserving surgery and 97% radiotherapy) with myofascial adhesions were evaluated using the developed tool by 2 independent raters...
2017: PloS One
https://www.readbyqxmd.com/read/28598492/use-of-2-octyl-cyanoacrylate-adhesive-and-polyester-mesh-for-wound-closure-in-primary-knee-arthroplasty
#14
Andrew J Holte, Josef N Tofte, Greta J Dahlberg, Nicolas Noiseux
Wound closure in primary knee arthroplasty is important both for surgical success, by minimizing the risk of wound complications and infection, and for patient satisfaction with wound care and cosmesis. This article reports the use of a topical self-adhering, pressure-sensitive polyester mesh combined with 2-octyl cyanoacrylate adhesive for final skin closure of primary knee arthroplasty without external staples or sutures. During a 2.5-year period, 360 consecutive primary knee arthroplasty procedures (321 total knee arthroplasty procedures and 39 partial knee arthroplasty procedures) performed by 1 surgeon at 1 institution were reviewed...
June 9, 2017: Orthopedics
https://www.readbyqxmd.com/read/28596238/loss-of-%C3%AE-pix-inhibits-focal-adhesion-disassembly-and-promotes-keratinocyte-motility-via-myosin-light-chain-activation
#15
Sho Hiroyasu, Gregory P Stimac, Susan B Hopkinson, Jonathan C R Jones
During healing of the skin, the cytoskeleton of keratinocytes and their matrix adhesions, including focal adhesions (FAs), undergo reorganization. These changes are coordinated by small GTPases and their regulators, including the guanine nucleotide exchange factor β-PIX. In fibroblasts, β-PIX activates small GTPases, thereby enhancing migration. In keratinocytes in vitro, β-PIX localizes to FAs. To study β-PIX functions, we generated β-PIX knockdown keratinocytes. During wound closure of β-PIX knockdown cell monolayers, disassembly of FA is impaired and their number and size are increased...
June 8, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28595249/a-prospective-randomized-single-blind-study-comparing-cyanoacrylate-adhesives-to-sutures-for-wound-closure-in-skin-cancer-patients
#16
Diana W Bartenstein, Deborah L Cummins, Gary S Rogers
BACKGROUND: Cyanoacrylate tissue adhesives are not more likely to result in wound dehiscence or infection than sutured closures. However, suturing is the gold standard for optimal cosmetic appearance of scars. OBJECTIVE: To determine whether cyanoacrylate tissue adhesives produce a cosmetic outcome equivalent to sutures at 3 months. Secondary outcomes include time for wound closure, time spent on wound care, and surgeon and patient satisfaction. METHODS AND MATERIALS: Seventy-one patients scheduled for skin cancer excision at Beverly Hospital were included in this prospective, randomized, single-blind study...
June 6, 2017: Dermatologic Surgery: Official Publication for American Society for Dermatologic Surgery [et Al.]
https://www.readbyqxmd.com/read/28593627/efficacy-and-safety-of-using-n-butyl-cyanoacrylate-in-fixation-of-cranial-following-trauma-and-other-pathologies
#17
Ahmed Sultan, Abbas Mohamed
Skull Bone fixation following different cranial surgeries is essential and should be rigid, simple and cheap. We describe our technique of cranial fixation using the adhesive cyanoacrylates. MATERIALS AND METHODS: At the end of cranial and intracranial surgeries the craniotomy flap and bone pieces are returned, realigned and fixed using Histoacryl® (N-Butyl Cyanoacrylate) glue. The glue is applied all around the flap in 360 degrees fashion. RESULT: The adhesive material was used in 24 cases after different surgeries involving the calvaria of the skull...
April 30, 2017: Turkish Neurosurgery
https://www.readbyqxmd.com/read/28591550/investigation-of-p120catenin-expression-in-human-basal-cell-carcinoma-of-the-skin
#18
Vladimír Bartoš, Milada Kullová
BACKGROUND: P120(ctn) is a specific membranous adhesion protein, that maintains the stability of intercellular junctions. An altered expression of p120(ctn), either reduced in the cell membrane or increase in the cytoplasm, plays a crucial role in carcinogenesis. No research has analysed the expression of p120(ctn) in basal cell carcinoma (BCC) of the skin so far. Therefore, we immunohistochemically studied p120(ctn) in a set of cutaneous BCCs in order to determine, whether there is difference in the expression pattern related to the histologic subtypes and tumor growth characteristics...
2017: Acta Medica (Hradec Králové)
https://www.readbyqxmd.com/read/28588334/use-of-a-non-invasive-surgical-skin-closure-device-in-dogs-following-dorsolateral-hemilaminectomy
#19
Christopher Corrie, Justin Shmalberg, Christine Senneca, Bobbi Conner
The ZipLine 16 surgical skin closure device (ZipLine 16; Zipline Medical, Campbell, California, USA) provides noninvasive surgical wound closure. This prospective study investigated its use in dogs undergoing a dorsolateral hemilaminectomy. Although the device produced normal appositional healing compared with controls, unacceptable traumatic dermatitis that is likely related to the device adhesive was noted after removal.
June 2017: Canadian Veterinary Journal. la Revue Vétérinaire Canadienne
https://www.readbyqxmd.com/read/28587970/bacterial-cellulose-in-biomedical-applications-a-review
#20
REVIEW
Guilherme Fadel Picheth, Cleverton Luiz Pirich, Maria Rita Sierakowski, Marco Aurélio Woehl, Caroline Novak Sakakibara, Clayton Fernandes de Souza, Andressa Amado Martin, Renata da Silva, Rilton Alves de Freitas
Bacterial cellulose (BC) derived materials represents major advances to the current regenerative and diagnostic medicine. BC is a highly pure, biocompatible and versatile material that can be utilized in several applications - individually or in the combination with different components (e.g. biopolymers and nanoparticles) - to provide structural organization and flexible matrixes to distinct finalities. The wide application and importance of BC is described by its common utilization as skin repair treatments in cases of burns, wounds and ulcers...
June 3, 2017: International Journal of Biological Macromolecules
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