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https://www.readbyqxmd.com/read/28629211/effect-of-methoxsalen-on-ca%C3%A2-%C3%A2-%C2%BA-homeostasis-and-viability-in-human-osteosarcoma-cells
#1
Yi-Chau Lu, Chiang-Ting Chou, Wei-Zhe Liang, Chun-Chi Kuo, Shu-Shong Hsu, Jue-Long Wang, Chung-Ren Jan
Methoxsalen is a natural compound found in many seed plants. The effect of methoxsalen on Ca²⁺- related physiology in human osteosarcoma is unclear. This study investigated the effect of methoxsalen on cytosolic free Ca²⁺ concentrations ([Ca²⁺]i) in MG63 human osteosarcoma cells. Methoxsalen induced [Ca²⁺]i rises concentration-dependently. Methoxsalen-induced Ca²⁺ entry was confirmed by Mn²⁺-induced quench of fura-2 fluorescence. This Ca²⁺ entry was suppressed by nifedipine, econazole, and SKF96365...
June 30, 2017: Chinese Journal of Physiology
https://www.readbyqxmd.com/read/28623463/hypotonicity-induced-cell-swelling-activates-trpa1
#2
Fumitaka Fujita, Kunitoshi Uchida, Yasunori Takayama, Yoshiro Suzuki, Masayuki Takaishi, Makoto Tominaga
Hypotonic solutions can cause painful sensations in nasal and ocular mucosa through molecular mechanisms that are not entirely understood. We clarified the ability of human TRPA1 (hTRPA1) to respond to physical stimulus, and evaluated the response of hTRPA1 to cell swelling under hypotonic conditions. Using a Ca(2+)-imaging method, we found that modulation of AITC-induced hTRPA1 activity occurred under hypotonic conditions. Moreover, cell swelling in hypotonic conditions evoked single-channel activation of hTRPA1 in a cell-attached mode when the patch pipette was attached after cell swelling under hypotonic conditions, but not before swelling...
June 16, 2017: Journal of Physiological Sciences: JPS
https://www.readbyqxmd.com/read/28595501/astragaloside-iv-inhibits-membrane-ca-formula-see-text-current-but-enhances-sarcoplasmic-reticulum-ca-formula-see-text-release
#3
Mei-Mi Zhao, Wen-Wen Lian, Zhuo Li, Dong-Xue Shao, Si-Chong Chen, Xue-Fei Sun, Hui-Yuan Hu, Rui Feng, Feng Guo, Li-Ying Hao
Astragaloside IV (AS-IV) is one of the active ingredients in Astragalus membrananceus (Huangqi), a traditional Chinese medicine. The present study investigated the effects of AS-IV on Ca[Formula: see text] handling in cardiac myocytes to elucidate its possible mechanism in the treatment of cardiac disease. The results showed that AS-IV at 1 and 10[Formula: see text][Formula: see text]M reduced KCl-induced [Ca[Formula: see text]]i increase ([Formula: see text] from 1.33[Formula: see text][Formula: see text][Formula: see text]0...
2017: American Journal of Chinese Medicine
https://www.readbyqxmd.com/read/28555330/the-p2y1-receptor-mediated-leukocyte-adhesion-to-endothelial-cells-is-inhibited-by-melatonin
#4
Tassya Cataldi Cardoso, Thaís Emanuelle Pompeu, Claudia Lucia Martins Silva
Extracellular ATP (released by endothelial and immune cells) and its metabolite ADP are important pro-inflammatory mediators via the activation of purinergic P2 receptors (P2Y and P2X), which represent potential new targets for anti-inflammatory therapy. Endothelial P2Y1 receptor (P2Y1R) induces endothelial cell activation triggering leukocyte adhesion. A number of data have implicated melatonin as a modulator of immunity, inflammation, and endothelial cell function, but to date no studies have investigated whether melatonin modulates endothelial P2YR signaling...
May 29, 2017: Purinergic Signalling
https://www.readbyqxmd.com/read/28554984/the-effect-of-deoxycholic-acid-on-secretion-and-motility-in-the-rat-and-guinea-pig-large-intestine
#5
Nam Hee Kim, Jung Ho Park, Jae-Soon Park, Yeun-Ho Joung
Background/Aims: Bile acid is an important luminal factor that affects gastrointestinal motility and secretion. We investigated the effect of bile acid on secretion in the proximal and distal rat colon and coordination of bowel movements in the guinea pig colon. Methods: The short-circuit current from the mucosal strip of the proximal and distal rat colon was compared under control conditions after induction of secretion with deoxycholic acid (DCA) as well as after inhibition of secretion with indomethacin, 1,2-bis (o-aminophenoxy) ethane-N, N,N',N'-tetra-acetic acid (an intracellular calcium chelator; BAPTA), and tetrodotoxin (TTX) using an Ussing chamber...
May 29, 2017: Journal of Neurogastroenterology and Motility
https://www.readbyqxmd.com/read/28550304/electronic-supplementary-materialidentification-of-a-na-k-atpase-inhibition-independent-proarrhythmic-ionic-mechanisms-of-cardiac-glycosides
#6
Cai Hong Koh, Jianjun Wu, Ying Ying Chung, Zhenfeng Liu, Rong-Rong Zhang, Ketpin Chong, Vladimir Korzh, Sherwin Ting, Steve Oh, Winston Shim, Hai-Yan Tian, Heming Wei
The current study explored the Na(+)/K(+)-ATPase (NKA) inhibition-independent proarrhythmic mechanisms of cardiac glycosides (CGs) which are well-known NKA inhibitors. With the cytosolic Ca(2+) chelated by EGTA and BAPTA or extracellular Ca(2+) replaced by Ba(2+), effects of bufadienolides (bufalin (BF) and cinobufagin (CBG)) and cardenolides (ouabain (Oua) and pecilocerin A (PEA)) on the L-type calcium current (I Ca,L) were recorded in heterologous expression Cav1.2-CHO cells and human embryonic stem cell-derived cardiomyocytes (hESC-CMs)...
May 26, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28530289/ca-nir-a-ratiometric-near-infrared-calcium-probe-based-on-a-dihydroxanthene-hemicyanine-fluorophore
#7
Mayeul Collot, Flavien Ponsot, Andrey S Klymchenko
Fluorescent calcium probes are essential tools for studying the fluctuation of calcium ions in cells. Herein, we developed Ca-NIR, the first ratiometric calcium probe emitting in the near infrared region. This probe arose from the fusion of a BAPTA chelator and a dihydroxanthene-hemicyanine fluorophore. It is efficiently excited with common 630-640 nm lasers and displays two distinct emission bands depending on the calcium concentration (Kd = ∼8 μM). The physicochemical and spectroscopic properties of Ca-NIR allowed for ratiometric imaging of calcium distribution in live cells...
June 1, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28521061/extracellular-camp-activates-molecular-signalling-pathways-associated-with-sperm-capacitation-in-bovines
#8
Carlos Agustín I Alonso, Claudia E Osycka-Salut, Luciana Castellano, Andreína Cesari, Nicolás Di Siervi, Adrián Mutto, Anders Johannisson, Jane M Morrell, Carlos Davio, Silvina Perez-Martinez
STUDY QUESTION: Is extracellular cAMP involved in the regulation of signalling pathways in bovine sperm capacitation? SUMMARY ANSWER: Extracellular cAMP-induced sperm capacitation through the activation of different signalling pathways that involve phospholipase C (PLC), PKC/ERK1-2 signalling and an increase in sperm Ca2+ levels, as well as soluble AC and cAMP/protein kinase A (PKA) signalling. WHAT IS KNOWN ALREADY: In order to fertilize the oocyte, ejaculated spermatozoa must undergo a series of changes in the female reproductive tract, known as capacitation...
May 18, 2017: Molecular Human Reproduction
https://www.readbyqxmd.com/read/28518134/asic1-and-asic3-contribute-to-acidity-induced-emt-of-pancreatic-cancer-through-activating-ca-2-rhoa-pathway
#9
Shuai Zhu, Hai-Yun Zhou, Shi-Chang Deng, Shi-Jiang Deng, Chi He, Xiang Li, Jing-Yuan Chen, Yan Jin, Zhuang-Li Hu, Fang Wang, Chun-You Wang, Gang Zhao
Extracellular acid can have important effects on cancer cells. Acid-sensing ion channels (ASICs), which emerged as key receptors for extracellular acidic pH, are differently expressed during various diseases and have been implicated in underlying pathogenesis. This study reports that ASIC1 and ASIC3 are mainly expressed on membrane of pancreatic cancer cells and upregulated in pancreatic cancer tissues. ASIC1 and ASIC3 are responsible for an acidity-induced inward current, which is required for elevation of intracellular Ca(2+) concentration ([Ca(2+)]i)...
May 18, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28506554/selective-dopamine-receptor-4-activation-mediates-the-hippocampal-neuronal-calcium-response-via-ip3-and-ryanodine-receptors
#10
Ya-Li Wang, Jian-Gang Wang, Fang-Li Guo, Xia-Huan Gao, Dan-Dan Zhao, Lin Zhang, Jian-Zhi Wang, Cheng-Biao Lu
Intracellular calcium is a key factor in most cellular processes, including cell growth, differentiation, proliferation and neurotransmitter release. Dopamine (DA) mediates synaptic transmission by regulating the intracellular calcium content. It is not clear, however, which specific subunit of the DA receptor contributes to DA modulation of intracellular calcium content changes. Through the traditional technique of Fura-2 calcium imaging, this study demonstrated that the DA can induce transient calcium in cultured hippocampal neurons and that this response can be mimicked by a selective dopamine receptor 4 (DR4) agonist PD168077 (PD)...
May 12, 2017: Brain Research
https://www.readbyqxmd.com/read/28494003/p2y6-receptors-are-involved-in-mediating-the-effect-of-inactivated-avian-influenza-virus-h5n1-on-il-6-cxcl8-mrna-expression-in-respiratory-epithelium
#11
Nawiya Huipao, Suparerk Borwornpinyo, Suwimon Wiboon-Ut, Craig R Campbell, Il-Ha Lee, Siriphun Hiranyachattada, Chonlaphat Sukasem, Arunee Thitithanyanont, Chumpol Pholpramool, David I Cook, Anuwat Dinudom
One of the key pathophysiologies of H5N1 infection is excessive proinflammatory cytokine response (cytokine storm) characterized by increases in IFN-β, TNF-α, IL-6, CXCL10, CCL4, CCL2 and CCL5 in the respiratory tract. H5N1-induced cytokine release can occur via an infection-independent mechanism, however, detail of the cellular signaling involved is poorly understood. To elucidate this mechanism, the effect of inactivated (β-propiolactone-treated) H5N1 on the cytokine and chemokine mRNA expression in 16HBE14o- human respiratory epithelial cells was investigated...
2017: PloS One
https://www.readbyqxmd.com/read/28477148/a-low-ca-2-i-induced-enhancement-of-camp-activated-ciliary-beating-by-pde1a-inhibition-in-mouse-airway-cilia
#12
Haruka Kogiso, Shigekuni Hosogi, Yukiko Ikeuchi, Saori Tanaka, Chikao Shimamoto, Hitoshi Matsumura, Takashi Nakano, Koh-Ichi Sano, Toshio Inui, Yoshinori Marunaka, Takashi Nakahari
This study demonstrated that PDE1 (phosphodiesterase 1) existing in the ciliary beat frequency (CBF)-regulating metabolon regulates CBF in procaterol-stimulated lung airway ciliary cells of mouse. Procaterol (an β2-agonist) increased the ciliary bend angle (CBA) and CBF via cAMP accumulation in the ciliary cells of mice: interestingly, the time course of CBF increase was slower than that of CBA increase. However, IBMX (3-isobutyl-1-methylxanthine, an inhibitor of PDE) increased CBA and CBF in an identical time course...
May 5, 2017: Pflügers Archiv: European Journal of Physiology
https://www.readbyqxmd.com/read/28474718/synthetic-water-soluble-di-tritopic-molecular-receptors-exhibiting-ca-2-mg-2-exchange
#13
Aurélie Lavie-Cambot, Arnaud Tron, Aurélien Ducrot, Frédéric Castet, Brice Kauffmann, Louis Beauté, Hassan Allouchi, Jean-Luc Pozzo, Célia S Bonnet, Nathan D McClenaghan
Structural integration of two synthetic water soluble receptors for Ca(2+) and Mg(2+), namely 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid (BAPTA) and o-aminophenol-N,N,O-triacetic acid (APTRA), respectively, gave novel di- and tritopic ionophores (1 and 2). As Mg(2+) and Ca(2+) cannot be simultaneously complexed by the receptors, allosteric control of complexation results. Potentiometric measurements established stepwise protonation constants and showed high affinity for Ca(2+) (log K = 6.08 and 8...
May 23, 2017: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/28468029/effect-of-protriptyline-on-ca%C3%A2-%C3%A2-%C2%BA-i-and-viability-in-mdck-renal-tubular-cells
#14
He-Hsiung Cheng, Chiang-Ting Chou, Wei-Zhe Liang, Chun-Chi Kuo, Pochuen Shieh, Jue-Long Wang, Chung-Ren Jan
Protriptyline has been used as an antidepressant. Clinically it has been prescribed in the auxiliary treatment of cancer patients. However, its effect on Ca²⁺ signaling and related physiology is unknown in renal cells. This study examined the effect of protriptyline on cytosolic free Ca²⁺ concentrations ([Ca²⁺]i) and viability in Madin-Darby canine kidney (MDCK) tubular cells. Protriptyline induced [Ca²⁺]i rises concentration-dependently. The response was reduced by 20% by removing extracellular Ca²⁺...
April 30, 2017: Chinese Journal of Physiology
https://www.readbyqxmd.com/read/28465084/monitoring-single-synapse-glutamate-release-and-presynaptic-calcium-concentration-in-organised-brain-tissue
#15
Thomas P Jensen, Kaiyu Zheng, Olga Tyurikova, James P Reynolds, Dmitri A Rusakov
Brain function relies in large part on Ca(2+)-dependent release of the excitatory neurotransmitter glutamate from neuronal axons. Establishing the causal relationship between presynaptic Ca(2+) dynamics and probabilistic glutamate release is therefore a fundamental quest across neurosciences. Its progress, however, has hitherto depended primarily on the exploration of either cultured nerve cells or giant central synapses accessible to direct experimental probing in situ. Here we show that combining patch-clamp with time-resolved imaging of Ca(2+) -sensitive fluorescence lifetime of Oregon Green BAPTA-1 (Tornado-FLIM) enables readout of single spike-evoked presynaptic Ca(2+) concentration dynamics, with nanomolar sensitivity, in individual neuronal axons in acute brain slices...
March 30, 2017: Cell Calcium
https://www.readbyqxmd.com/read/28458351/caffeine-suppresses-the-activation-of-hepatic-stellate-cells-camp-independently-by-antagonizing-adenosine-receptors
#16
Momoka Yamaguchi, Shin-Ya Saito, Ryota Nishiyama, Misuzu Nakamura, Kenichiro Todoroki, Toshimasa Toyo'oka, Tomohisa Ishikawa
During liver injury, hepatic stellate cells (HSCs) are activated by various cytokines and transdifferentiated into myofibroblast-like activated HSCs, which produce collagen, a major source of liver fibrosis. Therefore, the suppression of HSC activation is regarded as a therapeutic target for liver fibrosis. Several epidemiological reports have revealed that caffeine intake decreases the risk of liver disease. In this study, therefore, we investigated the effect of caffeine on the activation of primary HSCs isolated from mice...
2017: Biological & Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28436159/the-mechanism-research-of-hypoxia-induced-increased-late-sodium-current-resulting-in-intracellular-ca-2-overload-in-ventricular-myocytes
#17
Chen Fu, Jie Hao, Mengliu Zeng, Yejia Song, Wanzhen Jiang, Peihua Zhang, Antao Luo, Zhenzhen Cao, Luiz Belardinelli, Jihua Ma
Hypoxia leads to augmentation of late sodium current (INa.L ) and cellular Na(+) loading, increased reverse Na(+) -Ca(2+) exchange current (reverse INCX ) and intracellular Ca(2+) loading in rabbit ventricular myocytes. The purpose of this study was to determine the intracellular signal transduction pathways involved in the modulation of INa.L during hypoxia in ventricular myocytes. Whole-cell and cell-attached patch clamp techniques were used to record INa.L , and the whole-cell mode was also used to record reverse INCX and to study intercellular signal transduction mechanisms that mediate the increased INa...
April 24, 2017: Experimental Physiology
https://www.readbyqxmd.com/read/28431901/clozapine-reduces-toll-like-receptor-4-nf-%C3%AE%C2%BAb-mediated-inflammatory-responses-through-inhibition-of-calcium-calmodulin-dependent-akt-activation-in-microglia
#18
Seunghyun Jeon, Se Hyun Kim, Soon Young Shin, Young Han Lee
Clozapine is an atypical antipsychotic agent used in the treatment of schizophrenia and severe mood disorders. Accumulating evidence suggests that neuroinflammation is closely associated with the pathogenesis of various neurodegenerative diseases and psychiatric disorders. Clozapine exerts anti-inflammatory activity. However, the molecular mechanism underlying the anti-inflammatory activity of clozapine is poorly understood. In this study, we found that clozapine suppressed lipopolysaccharide (LPS)-induced phosphorylation of IκBα at Ser-32 and of p65/RelA at Ser-468, as well as nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB)-dependent transcriptional activity in microglial cells...
April 18, 2017: Progress in Neuro-psychopharmacology & Biological Psychiatry
https://www.readbyqxmd.com/read/28423696/improvement-of-dexamethasone-sensitivity-by-chelation-of-intracellular-ca2-in-pediatric-acute-lymphoblastic-leukemia-cells-through-the-prosurvival-kinase-erk1-2-deactivation
#19
Souleymane Abdoul-Azize, Isabelle Dubus, Jean-Pierre Vannier
Previous studies have demonstrated that glucocorticoid hormones, including dexamethasone, induced alterations in intracellular calcium homeostasis in acute lymphoblastic leukemia (ALL) cells. However, the mechanism by which intracellular calcium homeostasis participates in dexamethasone sensitivity and resistance on ALL cells remains elusive. Here, we found that treatment of cells with dexamethasone resulted in increased intracellular calcium concentrations through store-operated calcium entry stimulation, which was curtailed by store-operated calcium channel blockers...
April 18, 2017: Oncotarget
https://www.readbyqxmd.com/read/28419922/cadmium-induces-ca-2-mediated-calpain-1-caspase-3-dependent-apoptosis-in-primary-cultured-rat-proximal-tubular-cells
#20
Hong Wang, Nianhui Zhai, Ying Chen, Haibin Xu, Kehe Huang
Calcium, as a ubiquitous second messenger, governs a large array of cellular processes and is necessary for cell survival. More recently, it was observed that the cytosolic Ca(2+) concentration ([Ca(2+)]c) elevation could induce apoptosis in primary cultured rat proximal tubular (rPT) cells exposed to cadmium (Cd), but the concrete mechanism is still unclear. This study was designed to investigate the signal pathway involved in [Ca(2+)]c elevation-mediated apoptosis. The results confirmed the elevation of [Ca(2+)]c by confocal microscopy and enhancement of the apoptosis by Hoechst 33258 staining and flow cytometer when rPT cells were exposed to Cd for 12h...
April 8, 2017: Journal of Inorganic Biochemistry
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