Read by QxMD icon Read


Phu Hai Nguyen, Erika I Lutter, Ted Hackstadt
Chlamydia trachomatis is an obligate intracellular bacterium that replicates within a vacuole termed an inclusion. At the end of their intracellular developmental cycle, chlamydiae are released either by lysis of the host cell or extrusion of the intact inclusion. The inclusion membrane is extensively modified by the insertion of type III secreted inclusion membrane proteins, Incs, which contribute to inclusion membrane structure and facilitate host-pathogen interactions. An interaction was identified between the inclusion membrane protein, MrcA, and the Ca2+ channel inositol-1,4,5-trisphosphate receptor, type 3 (ITPR3)...
March 2018: PLoS Pathogens
Shanshan Feng, Qiaozhi Zhou, Bo Yang, Qianqian Li, Aiqin Liu, Yingying Zhao, Changqing Qiu, Jun Ge, Huihong Zhai
BACKGROUND: Calcyclin Binding Protein/(Siah-1 interacting protein) (CacyBP/SIP) acts as an oncogene in colorectal cancer. The nuclear accumulation of CacyBP/SIP has been linked to the proliferation of cancer cells. It has been reported that intracellular Ca2+ induces the nuclear translocation of CacyBP/SIP. However, the molecular mechanism of CacyBP/SIP nuclear translocation has yet to be elucidated. The purpose of this study was to test whether the Ca2+-dependent binding partner S100 protein is involved in CacyBP/SIP nuclear translocation in colon cancer SW480 cells...
2018: PloS One
Alfredo Criollo, Francisco Altamirano, Zully Pedrozo, Gabriele G Schiattarella, Dan L Li, Pablo Rivera-Mejías, Cristian Sotomayor-Flores, Valentina Parra, Elisa Villalobos, Pavan K Battiprolu, Nan Jiang, Herman I May, Eugenia Morselli, Stefan Somlo, Humberto de Smedt, Thomas G Gillette, Sergio Lavandero, Joseph A Hill
AIMS: Considerable evidence points to critical roles of intracellular Ca2+ homeostasis in the modulation and control of autophagic activity. Yet, underlying molecular mechanisms remain unknown. Mutations in the gene (pkd2) encoding polycystin-2 (PC2) are associated with autosomal dominant polycystic kidney disease (ADPKD), the most common inherited nephropathy. PC2 has been associated with impaired Ca2+ handling in cardiomyocytes and indirect evidence suggests that this protein may be involved in autophagic control...
March 5, 2018: Journal of Molecular and Cellular Cardiology
Haruka Kogiso, Yukiko Ikeuchi, Masako Sumiya, Shigekuni Hosogi, Saori Tanaka, Chikao Shimamoto, Toshio Inui, Yoshinori Marunaka, Takashi Nakahari
Sei-hai-to (TJ-90, Qing Fei Tang), a Chinese traditional medicine, increases ciliary beat frequency (CBF) and ciliary bend angle (CBA) mediated via cAMP (3',5'-cyclic adenosine monophosphate) accumulation modulated by Ca2+ -activated phosphodiesterase 1 (PDE1A). A high concentration of TJ-90 (≥40 μg/mL) induced two types of CBF increases, a transient increase (an initial increase, followed by a decrease) and a sustained increase without any decline, while it only sustained the CBA increase. Upon inhibiting increases in intracellular Ca2+ concentration ([Ca2+ ]i ) by 10 μM BAPTA-AM (Ca2+ -chelator, 1,2-Bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid tetrakis(acetoxymethyl ester) or Ca2+ /calmodulin-dependent PDE1 by 8MmIBMX (a selective PDE1 inhibitor), TJ-90 (400 μg/mL) induced only the sustained CBF increase without any transient CBF increase...
February 26, 2018: International Journal of Molecular Sciences
Noriyuki Hatano, Susumu Ohya, Yuji Imaizumi, Robert B Clark, Darrell Belke, Wayne R Giles
KEY POINTS: Fibroblasts isolated from adult rat ventricles consistently respond to (5-100 μM) ATP. ATP, ADP, and UTP all elicit similar dose-dependent increases in [Ca2+ ]i . ATP also activates an outwardly rectifying Cl- current; in contrast, ADP and UTP have much smaller electrophysiological effects. This ATP-dependent Cl- current is markedly reduced when: i) [Ca2+ ]i is buffered with BAPTA, or ii) the intrinsic phospholipase C activity in these fibroblasts is inhibited. PCR analysis suggests that the ATP-induced current is generated by the Ca2+ -activated Cl- current transcript TMEM 16F, also denoted ANO6...
March 1, 2018: Experimental Physiology
Jaya Gautam, Suhrid Banskota, Sajita Shah, Jun-Goo Jee, Eunju Kwon, Ying Wang, Dong Young Kim, Hyun Wook Chang, Jung-Ae Kim
BACKGROUND AND PURPOSE: In this study, we examined that 4-hydroxynonenal (4-HNE) acts as a ligand for GPR109A, which elicits both anti-inflammatory and cell death responses. EXPERIMENTAL APPROACH: Agonistic activity of 4-HNE was determined by observing the inhibition of cAMP generation in CHO-K1-GPR109A-Gi cell line, surface plasmon resonance (SPR) binding assay, and competition binding assay with [3 H]niacin. 4-HNE-mediated signaling pathways and cellular responses were investigated in GPR109A-expressing and -non-expressing cells...
February 23, 2018: British Journal of Pharmacology
Shu-Feng Hsieh, Chiang-Ting Chou, Wei-Zhe Liang, Chun-Chi Kuo, Jue-Long Wang, Lyh-Jyh Hao, Chung-Ren Jan
OBJECTIVE: Magnolol, a polyphenol compound from herbal medicines, was shown to alter physiology in various cell models. However, the effect of magnolol on Ca2+ homeostasis and its related physiology in oral cancer cells is unclear. This study examined whether magnolol altered Ca2+ signaling and cell viability in OC2 human oral cancer cells. METHODS: Cytosolic Ca2+ concentrations ([Ca2+ ]i ) in suspended cells were measured by using the fluorescent Ca2+ -sensitive dye fura-2...
February 14, 2018: Archives of Oral Biology
Shuhua Zheng, Gilles M Leclerc, Bin Li, Ronan T Swords, Julio C Barredo
De novo and acquired drug resistance and subsequent relapse remain major challenges in acute lymphoblastic leukemia (ALL). We previously identified that pevonedistat (TAK-924, MLN4924), a first-in-class inhibitor of NEDD8 activating enzyme (NAE), elicits ER stress and has potent in vitro and in vivo efficacy against ALL. However, in pevonedistat-treated ALL cell lines, we found consistent activation of the pro-survival MEK/ERK pathway, which has been associated with relapse and poor outcome in ALL. We uncovered that inhibition of the MEK/ERK pathway in vitro and in vivo sensitized ALL cells to pevonedistat...
January 19, 2018: Oncotarget
Teng Wang, Kuan Ning, Xu Sun, Chun Zhang, Lin-Fang Jin, Dong Hua
BACKGROUND: Elevated intracellular Ca2+ ([Ca2+ ]i ) level could lead to [Ca2+ ]i overload and promote apoptosis via different pathways. In our previously study, up-regulated expression of transient receptor potential canonical channel (TRPC5) was proven to increase [Ca2+ ]i level, and resulted in chemoresistance whereas not apoptosis in human colorectal cancer (CRC) cells. The ATP-dependent homeostatic maintenance of resting [Ca2+ ]i should be important in this process. Increased glycolysis was found to be an important adenosine triphosphate (ATP) source in cancer...
February 20, 2018: BMC Cancer
Kazuki Noma, Hironari Akaike, Yuki Kurauchi, Hiroshi Katsuki, Yasuo Oyama, Norio Akaike
Glycine is a fast inhibitory transmitter like γ-aminobutyric acid in the mammalian spinal cord and brainstem, and it is involved in motor reflex, nociception, and neuronal development. Triphenyltin (TPT) is an organometallic compound causing environmental hazard to many wild creatures. Our previous findings show that TPT ultimately induces a drain and/or exhaustion of glutamate in excitatory presynaptic nerve terminals, resulted in blockage of neurotransmission as well as methylmercury. Therefore, we have investigated the neurotoxic mechanism how TPT modulates inhibitory glycinergic transmission in the synaptic bouton preparation of rat isolated spinal neurons using a patch clamp technique...
February 13, 2018: Environmental Research
Ji Hee Lim, Hyung Wook Kim, Min Young Kim, Tae Woo Kim, Eun Nim Kim, Yaeni Kim, Sungjin Chung, Young Soo Kim, Bum Soon Choi, Yong-Soo Kim, Yoon Sik Chang, Hye Won Kim, Cheol Whee Park
Apoptosis and autophagy are harmoniously regulated biological processes for maintaining tissue homeostasis. AMP-activated protein kinase (AMPK) functions as a metabolic sensor to coordinate cellular survival and function in various organs, including the kidney. We investigated the renoprotective effects of cinacalcet in high-glucose treated human glomerular endothelial cells (HGECs), murine podocytes and C57BLKS/J-db/db mice. In cultured HGECs and podocytes, cinacalcet decreased oxidative stress and apoptosis and increased autophagy that were attributed to the increment of intracellular Ca2+ concentration and the phosphorylation of Ca2+ /calmodulin-dependent protein kinase kinaseβ (CaMKKβ)-Liver kinase B1 (LKB1)-AMPK and their downstream signals including the phosphorylation of endothelial nitric oxide synthase (eNOS) and increases in superoxide dismutases and B cell leukemia/lymphoma 2/BCL-2-associated X protein expression...
February 15, 2018: Cell Death & Disease
Emily K Stephens, Arielle L Baker, Allan T Gulledge
Serotonin (5-HT) selectively excites subpopulations of pyramidal neurons in the neocortex via activation of 5-HT2A (2A) receptors coupled to Gq subtype G-protein alpha subunits. Gq-mediated excitatory responses have been attributed primarily to suppression of potassium conductances, including those mediated by KV7 potassium channels (i.e., the M-current), or activation of non-specific cation conductances that underlie calcium-dependent afterdepolarizations (ADPs). However, 2A-dependent excitation of cortical neurons has not been extensively studied, and no consensus exists regarding the underlying ionic effector(s) involved...
2018: Frontiers in Neural Circuits
Yang Long, Shao-Wei Chen, Chen-Lin Gao, Xue-Mei He, Guan-Nan Liang, Jian Wu, Chun-Xia Jiang, Xin Liu, Fang Wang, Feng Chen
Emerging epidemiological and experimental evidence has shown that the ATP2B1 gene is associated with blood pressure control. Impaired eNOS activity and NO production may be among the mechanisms involved. However, little is known about how PMCA1, which is encoded by the ATP2B1 gene, regulates the activity of eNOS and NO production. In the present study, we investigated the role of the ATP2B1 gene in regulating eNOS activity and NO production under basal conditions in HUVECs and explored the mechanisms involved...
February 7, 2018: Hypertension Research: Official Journal of the Japanese Society of Hypertension
Yoskaly Lazo-Fernandez, Paul A Welling, Susan M Wall
Alpha-ketoglutarate (αKG) is a citric acid cycle intermediate and a glutamine catabolism product. It is also the natural ligand of OXGR1, a Gq-protein coupled receptor expressed on the apical membrane of intercalated cells. In the CCD, Cl- /HCO3 - exchange increases upon αKG binding to the OXGR1. To determine the signaling pathway(s) by which αKG stimulates Cl- absorption, we examined αKG-stimulated Cl- absorption in isolated perfused mouse CCDs. αKG increased electroneutral Cl- absorption in CCDs from wild type mice, but had no effect on Cl- absorption in pendrin KO mice...
February 7, 2018: American Journal of Physiology. Renal Physiology
Xi Zhang, Xiaogang Wang, Taixiu Liu, Min Mo, Lin Ao, Jinyi Liu, Jia Cao, Zhihong Cui
Sertoli cells (SCs) provide physical and nutritional support for spermatogenesis. Dibutyl phthalate (DBP) is a plasticizer that has male reproductive toxicity. The collapse of vimentin in DBP-exposed SCs is thought to induce the sloughing of spermatocytes from seminiferous tubules. In this study, we explored methods to rescue vimentin from collapse in DBP-exposed SCs. DBP not only induced the hyperphosphorylation of vimentin but also triggered endoplasmic reticulum (ER) stress and apoptosis in SCs. Treatment with BAPTA-AM, an antagonist of Ca2+, significantly decreased the level of phosphorylated vimentin, while LY294002, an inhibitor of Akt1, did not...
January 31, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
Mozibur Rahman, Rui Sun, Subhendu Mukherjee, Bernd Nilius, Luke J Janssen
We previously described several ionic conductances in human pulmonary fibroblasts (HPFs), including one activated by two structurally distinct TRPV4-channel agonists: 4α-phorbol-12,13-didecanoate (4αPDD) and GSK1016790A. However, the TRPV4-activated current exhibited peculiar properties: it developed slowly over many minutes, exhibited reversal potentials that could vary by tens of millivolts even within a given cell, and was not easily reversed by subsequent addition of two distinct TRPV4-selective blockers (RN-1734 or HC-067047)...
February 2, 2018: American Journal of Respiratory Cell and Molecular Biology
Bon-Hyeock Koo, Bong-Gu Yi, Myeong-Seon Jeong, Seung-Hea Kwon, Kwang-Lae Hoe, Young-Guen Kwon, Moo-Ho Won, Young-Myeong Kim, Sungwoo Ryoo
Arginase inhibition exhibits beneficial effects in vascular endothelial and smooth muscle cells. In human aortic smooth muscle cells (hAoSMCs), native low-density lipoprotein (nLDL) induced the production of interleukin-8 (IL-8) that is involved in the pathogenesis of cardiovascular diseases. Therefore, we examined the effect of arginase inhibition on IL-8 production and the underlying mechanism. In hAoSMCs, reverse transcription-PCR, western blotting and immunocytochemistry with MitoTracker confirmed that arginase II was confined predominantly to mitochondria...
February 2, 2018: Experimental & Molecular Medicine
Nathan A Smith, Benjamin T Kress, Yuan Lu, Devin Chandler-Militello, Abdellatif Benraiss, Maiken Nedergaard
Fluorescent Ca2+ indicators have been essential for the analysis of Ca2+ signaling events in various cell types. We showed that chemical Ca2+ indicators, but not a genetically encoded Ca2+ indicator, potently suppressed the activity of Na+- and K+-dependent adenosine triphosphatase (Na,K-ATPase), independently of their Ca2+ chelating activity. Loading of commonly used Ca2+ indicators, including Fluo-4 acetoxymethyl (AM), Rhod-2 AM, and Fura-2 AM, and of the Ca2+ chelator BAPTA AM into cultured mouse or human neurons, astrocytes, cardiomyocytes, or kidney proximal tubule epithelial cells suppressed Na,K-ATPase activity by 30 to 80%...
January 30, 2018: Science Signaling
Sara Damiano, Serena Montagnaro, Maria Valeria Puzio, Lorella Severino, Ugo Pagnini, Marcella Barbarino, Daniele Cesari, Antonio Giordano, Salvatore Florio, Roberto Ciarcia
In clinical practice for the treatment of chronic myeloid leukemia, second generation of tyrosine kinase inhibitors such as Nilotinib (NIL) specific and potent inhibitor of the BCR/ABL kinase and Dasatinib (DAS) a inhibitor of BCR/ABL and Src family kinase were developed to clinically overcome imatinib resistance. In this study we wanted to test the ability of some antioxidants such Resveratrol (RES) or a new recombinant mitochondrial manganese containing superoxide dismutase (rMnSOD) or δ-tocotrienol (δ-TOCO) to interact with DAS and NIL on viability, reactive oxygen species (ROS) production, lipid peroxidation and apoptosis...
January 18, 2018: Journal of Cellular Biochemistry
Tamara Vervloessem, Haidar Akl, Thomas Tousseyn, Humbert De Smedt, Jan B Parys, Geert Bultynck
Bcl-2 is often upregulated in cancers to neutralize the BH3-only protein Bim at the mitochondria. BH3 mimetics (e.g. ABT-199 (venetoclax)) kill cancer cells by targeting Bcl-2's hydrophobic cleft and disrupting Bcl-2/Bim complexes. Some cancers with elevated Bcl-2 display poor responses towards BH3 mimetics, suggesting an additional function for anti-apoptotic Bcl-2 in these cancers. Indeed, Bcl-2 via its BH4 domain prevents cytotoxic Ca2+ release from the endoplasmic reticulum (ER) by directly inhibiting the inositol 1,4,5-trisphosphate receptor (IP3R)...
December 19, 2017: Oncotarget
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"