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https://www.readbyqxmd.com/read/29345355/effects-of-antioxidants-on-apoptosis-induced-by-dasatinib-and-nilotinib-in-k562-cells
#1
Sara Damiano, Serena Montagnaro, Maria Valeria Puzio, Lorella Severino, Ugo Pagnini, Marcella Barbarino, Daniele Cesari, Antonio Giordano, Salvatore Florio, Roberto Ciarcia
In clinical practice for the treatment of chronic myeloid leukemia, second generation of tyrosine kinase inhibitors such as Nilotinib (NIL) specific and potent inhibitor of the BCR/ABL kinase and Dasatinib (DAS) a inhibitor of BCR/ABL and Src family kinase were developed to clinically overcome imatinib resistance. In this study we wanted to test the ability of some antioxidants such Resveratrol (RES) or a new recombinant mitochondrial manganese containing superoxide dismutase (rMnSOD) or δ-tocotrienol (δ-TOCO) to interact with DAS and NIL on viability, reactive oxygen species (ROS) production, lipid peroxidation and apoptosis...
January 18, 2018: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29340082/reciprocal-sensitivity-of-diffuse-large-b-cell-lymphoma-cells-to-bcl-2-inhibitors-bird-2-versus-venetoclax
#2
Tamara Vervloessem, Haidar Akl, Thomas Tousseyn, Humbert De Smedt, Jan B Parys, Geert Bultynck
Bcl-2 is often upregulated in cancers to neutralize the BH3-only protein Bim at the mitochondria. BH3 mimetics (e.g. ABT-199 (venetoclax)) kill cancer cells by targeting Bcl-2's hydrophobic cleft and disrupting Bcl-2/Bim complexes. Some cancers with elevated Bcl-2 display poor responses towards BH3 mimetics, suggesting an additional function for anti-apoptotic Bcl-2 in these cancers. Indeed, Bcl-2 via its BH4 domain prevents cytotoxic Ca2+ release from the endoplasmic reticulum (ER) by directly inhibiting the inositol 1,4,5-trisphosphate receptor (IP3R)...
December 19, 2017: Oncotarget
https://www.readbyqxmd.com/read/29298904/multiple-lines-of-evidence-indicate-that-gliotransmission-does-not-occur-under-physiological-conditions
#3
Todd A Fiacco, Ken D McCarthy
A major controversy persists within the field of glial biology concerning whether or not, under physiological conditions, neuronal activity leads to Ca2+-dependent release of neurotransmitters from astrocytes, a phenomenon known as gliotransmission. Our perspective is that, while we and others can apply techniques to cause gliotransmission, there is considerable evidence gathered using astrocyte-specific and more physiological approaches which suggests that gliotransmission is a pharmacological phenomenon rather than a physiological process...
January 3, 2018: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/29282782/-ca2-i-modulation-of-camp-stimulated-ciliary-beat-frequency-via-pde1-in-airway-ciliary-cells-of-mice
#4
Haruka Kogiso, Shigekuni Hosogi, Yukiko Ikeuchi, Saori Tanaka, Toshio Inui, Yoshinori Marunaka, Takashi Nakahari
Intracellular Ca2+ concentration ([Ca2+ ]i ) plays crucial roles in the regulation of ciliary beat frequency (CBF) and ciliary bend angle (CBA) of airway cilia. Moreover, Ca2+ -dependent PDE1A exists in the CBF regulating metabolon of cilia. This study demonstrated that CBF is regulated by a direct and an indirect action of [Ca2+ ]i ; the direct action changes CBF mediated via [Ca2+ ]i and the indirect action changes CBF mediated via cAMP, accumulation of which is controlled by the PDE1 activity. Upon reducing [Ca2+ ]i to various levels, the direct action decreases CBF and the indirect action increases CBF...
December 28, 2017: Experimental Physiology
https://www.readbyqxmd.com/read/29248592/fluorochloridone-induces-primary-cultured-sertoli-cells-apoptosis-involvement-of-ros-and-intracellular-calcium-ions-mediated-erk1-2-activation
#5
Luqing Liu, Xiuli Chang, Yubin Zhang, Chunhua Wu, Rui Li, Liming Tang, Zhijun Zhou
Fluorochloridone (FLC) is a widely used pyrrolidone selective herbicide and reported to induce testis injuries in male rats, but the underlying mechanism is largely unknown. In the present study, primary-cultured Sertoli cells were exposed to FLC at the concentration of 0-10.00μM to study the mechanism of FLC-induced apoptosis. The roles of ROS, intracellular calcium, endoplasmic reticulum (ER), and ERK1/2 were looked at with ROS scavenger N-acetyl-cysteine (NAC), intracellular calcium chelator BAPTA-AM, ER calcium depleting agent thapsigargin (TG), and ERK1/2 inhibitor U0126, respectively...
December 14, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29248464/decanoic-acid-suppresses-proliferation-and-invasiveness-of-human-trophoblast-cells-by-disrupting-mitochondrial-function
#6
Changwon Yang, Whasun Lim, Fuller W Bazer, Gwonhwa Song
Decanoic acid (DA) is a medium-chain fatty acid used in the manufacture of various products including plastics, cosmetics, and lubricants. In addition to antiviral and antibacterial effects, DA's, reported biological activities include regulation of signaling pathways and redox homeostasis in various human cell types. The influence of DA on functional properties of human trophoblasts, including proliferation, invasion and apoptosis is currently unknown. In the present study, we evaluated the anti-proliferative and anti-invasive effects of DA on the human trophoblast cell line HTR8/SVneo...
December 14, 2017: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/29245916/laminarin-counteracts-diet-induced-obesity-associated-with-glucagon-like-peptide-1-secretion
#7
Liusong Yang, Lina Wang, Canjun Zhu, Junguo Wu, Yexian Yuan, Lulu Yu, Yaqiong Xu, Jingren Xu, Tao Wang, Zhengrui Liao, Songbo Wang, Xiaotong Zhu, Ping Gao, Yongliang Zhang, Xiuqi Wang, Qingyan Jiang, Gang Shu
Laminarin, a type of β-glucan isolated from brown seaweeds, exhibits verity of physiological activities, which include immunology modulation and antitumor function. To investigate the effect of laminarin on energy homeostasis, mice were orally administrated with laminarin to test food intake, fat deposition, and glucose homeostasis. Chronically, laminarin treatment significantly decreases high-fat-diet-induced body weight gain and fat deposition and reduces blood glucose level and glucose tolerance. Acutely, laminarin enhances serum glucagon-like peptide-1 (GLP-1) content and the mRNA expression level of proglucagon and prohormone convertase 1 in ileum...
November 21, 2017: Oncotarget
https://www.readbyqxmd.com/read/29226878/asic1a-contributes-to-the-symptom-of-pain-in-a-rat-model-of-chronic-prostatitis
#8
Song Fan, Zong-Yao Hao, Li Zhang, Jun Zhou, Yi-Fei Zhang, Shen Tai, Xian-Sheng Zhang, Chao-Zhao Liang
This study aims to validate our hypothesis that acid-sensing ion channels (ASICs) may contribute to the symptom of pain in patients with chronic prostatitis (CP). We first established a CP rat model, then isolated the L5-S2 spinal dorsal horn neurons for further studies. ASIC1a was knocked down and its effects on the expression of neurogenic inflammation-related factors in the dorsal horn neurons of rat spinal cord were evaluated. The effect of ASIC1a on the Ca2+ ion concentration in the dorsal horn neurons of rat spinal cord was measured by the intracellular calcium ([Ca2+]i) intensity...
December 8, 2017: Asian Journal of Andrology
https://www.readbyqxmd.com/read/29217191/tnf-%C3%AE-induces-expression-of-the-circadian-clock-gene-bmal1-via-dual-calcium-dependent-pathways-in-rheumatoid-synovial-cells
#9
Kohsuke Yoshida, Ayako Nakai, Kenta Kaneshiro, Naonori Hashimoto, Kohjin Suzuki, Koto Uchida, Teppei Hashimoto, Yoshiko Kawasaki, Koji Tateishi, Natsuko Nakagawa, Nao Shibanuma, Yoshitada Sakai, Akira Hashiramoto
Tumor necrosis factor (TNF)-α is responsible for expressions of several clock genes and affects joint symptoms of rheumatoid arthritis (RA) with diurnal fluctuation. We tried to determine the mechanism involved in over-expression of Bmal1, induced by TNF-α, in primary cultured rheumatoid synovial cells. Cells were incubated with intra-cellular Ca2+ chelator BAPTA-AM, calcineurin inhibitor FK506 and p300/CBP (CREB binding protein) inhibitor C646, respectively, or transfected with p300 and CBP small interfering RNA (siRNA) before stimulation with TNF-α...
December 4, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29208466/chetomin-induces-apoptosis-in-human-triple-negative-breast-cancer-cells-by-promoting-calcium-overload-and-mitochondrial-dysfunction
#10
Jayant Dewangan, Sonal Srivastava, Sakshi Mishra, Prabhash Kumar Pandey, Aman Divakar, Srikanta Kumar Rath
Human triple-negative breast cancer (TNBC) is poorly diagnosed and unresponsive to conventional hormone therapy. Chetomin (CHET), a fungal metabolite synthesized by Chaetomium cochliodes, has been reported as a promising anticancer and antiangiogenic agent but the complete molecular mechanism of its anticancer potential remains to be elucidated. In our study, we explored the anti-neoplastic action of CHET on TNBC cells. Cytotoxicity studies were performed in human TNBC cells viz. MDA-MB-231 and MDA-MB-468 cells by Sulforhodamine B assay...
December 2, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29207633/camkii-is-involved-in-subcellular-ca2-redistribution-induced-endoplasmic-reticulum-stress-leading-to-apoptosis-in-primary-cultures-of-rat-proximal-tubular-cells-exposed-to-lead
#11
Min-Ge Wang, Wen-Hui Li, Xin-Yu Wang, Du-Bao Yang, Zhen-Yong Wang, Lin Wang
Lead (Pb) is a known nephrotoxic element. Recently we have proved that subcellular Ca2+ redistribution is involved in Pb-induced apoptosis in primary cultures of rat proximal tubular (rPT) cells, but the underlying mechanism remains to be elucidated. Firstly, data showed that Pb triggers endoplasmic reticulum (ER) stress response in rPT cells, as evidenced by the elevations of ER stress markers. Moreover, pharmacological modulation of Ca2+ mobilization in ER and cytoplasm with three chemicals (2-APB or TG or BAPTA-AM) can effectively increase or decrease the protein expression of ER stress markers in Pb-exposed rPT cells, demonstrating that Pb-induced ER stress is Ca2+-dependent...
October 31, 2017: Oncotarget
https://www.readbyqxmd.com/read/29205619/bapta-am-dramatically-improves-maturation-and-development-of-bovine-oocytes-from-grade-3-cumulus-oocyte-complexes
#12
Hongmei Hu, Xianhong Mo, Xue Li, Xiangwei Fu, Yunpeng Hou
Intracellular free calcium ([Ca2+ ]i ) is essential for oocyte maturation and early embryonic development. Here, we investigated the role of [Ca2+ ]i in oocytes from cumulus-oocyte complexes (COCs) with respect to maturation and early embryonic development, using the calcium-buffering agent BAPTA-AM (1,2-bis[2-aminophenoxy]ethane-N,N,N',N'-tetraacetic acid tetrakis [acetoxymethyl ester]). COCs were graded based on compactness of the cumulus mass and appearance of the cytoplasm, with Grade 1 indicating higher quality and developmental potential than Grade 3...
December 4, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/29194002/auranofin-a-thioredoxin-reductase-inhibitor-causes-platelet-death-through-calcium-overload
#13
Matthew T Harper
Platelets are central to normal hemostasis and must be tightly controlled to prevent thrombosis. However, drug treatments that also affect platelets could lead to unwanted side effects on hemostasis or thrombosis. In this study, the effect of auranofin on platelets was tested. Auranofin, a gold-based thioredoxin reductase (TRXR) inhibitor, has been previously used in arthritis. Recently, auranofin and other inhibitors of the thioredoxin system have been proposed as novel anti-cancer therapies. TRXR is an important part of the antioxidant defenses in many cells that maintain intracellular proteins in their reduced state...
December 1, 2017: Platelets
https://www.readbyqxmd.com/read/29173277/exogenous-tumor-necrosis-factor-alpha-could-induce-egress-of-toxoplasma%C3%A2-gondii-from-human-foreskin-fibroblast-cells
#14
Yong Yao, Miao Liu, Cuiping Ren, Jijia Shen, Yongsheng Ji
Toxoplasma gondii is an intra-cellular protozoan parasite that can infect almost all nucleated cells, eliciting host immune responses against infection. Host tissue damage is mainly caused by cellular lysis when T. gondii egresses from infected cells. However, the effects of cytokines released by host immune cells on egression of T. gondii remain elusive. This study aimed to investigate the role of tumor necrosis factor-alpha (TNF-α) on the egress of T. gondii from infected human foreskin fibroblast (HFF) cells and to elucidate the underlying mechanisms that regulate TNF-α-induced egress...
2017: Parasite: Journal de la Société Française de Parasitologie
https://www.readbyqxmd.com/read/29165917/a-novel-multisite-confocal-system-for-rapid-ca-2-imaging-from-submicron-structures-in-brain-slices
#15
Luiza Filipis, Karima Ait Ouares, Philippe Moreau, Dimitrii Tanese, Valeria Zampini, Andrea Latini, Chun Bleau, Charlie Bleau, Jeremy Graham, Marco Canepari
In brain slices, resolving fast Ca(2+) fluorescence signals from submicron structures is typically achieved using two-photon or confocal scanning microscopy, an approach that limits the number of scanned points. The novel multiplexing confocal system presented here overcomes this limitation. This system is based on a fast spinning disk, a multimode diode laser and a novel high-resolution CMOS camera. The spinning disk, running at 20,000 rpm, has custom-designed spiral pattern that maximises light collection while rejecting out-of-focus fluorescence to resolve signals from small neuronal compartments...
November 22, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/29155306/reverse-t3-interacts-with-%C3%AE-v%C3%AE-3-integrin-receptor-and-restores-enzyme-activities-in-the-hippocampus-of-hypothyroid-developing-rats-insight-on-signaling-mechanisms
#16
Juliana Tonietto Domingues, Daiane Cattani, Patricia Acordi Cesconetto, Bianka Alzira Nascimento de Almeida, Paula Peirozan, Guilherme Razzera, Fátima Regina Mena Barreto Silva, Regina Pessoa-Pureur, Ariane Zamoner
In the present study we provide evidence that 3,3',5'-triiodothyronine (reverse T3, rT3) restores neurochemical parameters induced by congenital hypothyroidism in rat hippocampus. Congenital hypothyroidism was induced by adding 0.05% propylthiouracil in the drinking water from gestation day 8 and continually up to lactation day 15. In the in vivo rT3 exposure, hypothyroid 12-day old pups were daily injected with rT3 (50 ng/kg body weight) or saline until day 14. In the ex vivo rT3 treatment, hippocampal slices from 15-day-old hypothyroid pups were incubated for 30 min with or without rT3 (1 nM)...
November 16, 2017: Molecular and Cellular Endocrinology
https://www.readbyqxmd.com/read/29142096/novel-ca-2-dependent-mechanisms-regulate-spontaneous-release-at-excitatory-synapses-onto-ca1-pyramidal-cells
#17
Walter E Babiec, Thomas J O'Dell
Although long thought to simply be a source of synaptic noise, spontaneous, action-potential independent release of neurotransmitter from presynaptic terminals has multiple roles in synaptic function. We explored whether and to what extent the two predominantly proposed mechanisms for explaining spontaneous release, stochastic activation of voltage-gated Ca(2+) channels (VGCCs) or activation of Ca(2+)-sensing receptors (CaSRs) by extracelluar Ca(2+), played a role in the sensitivity of spontaneous release to the level of extracellular Ca(2+) concentration at excitatory synapses at CA1 pyramidal cells of the adult, male mouse hippocampus...
November 15, 2017: Journal of Neurophysiology
https://www.readbyqxmd.com/read/29138358/epileptic-pilocarpine-treated-rats-exhibit-aberrant-hippocampal-epsp-spike-potentiation-but-retain-long-term-potentiation
#18
Ezekiel Carpenter-Hyland, Edyta K Bichler, Mathew Smith, Robert S Sloviter, Morris Benveniste
Hippocampal neuron plasticity is strongly associated with learning, memory, and cognition. In addition to modification of synaptic function and connectivity, the capacity of hippocampal neurons to undergo plasticity involves the ability to change nonsynaptic excitability. This includes altering the probability that EPSPs will generate action potentials (E-S plasticity). Epilepsy is a prevalent neurological disorder commonly associated with neuronal hyperexcitability and cognitive dysfunction. We examined E-S plasticity in chronically epileptic Sprague-Dawley rats 3-10 weeks after pilocarpine-induced status epilepticus CA1 neurons in hippocampal slices were assayed by whole-cell current clamp to measure EPSPs evoked by Schaffer collateral stimulation...
November 2017: Physiological Reports
https://www.readbyqxmd.com/read/29138225/androgen-signaling-expands-%C3%AE-cell-mass-in-male-rats-and-%C3%AE-cell-androgen-receptor-is-degraded-under-high-glucose-conditions
#19
Naoki Harada, Yasuhiro Yoda, Yusuke Yotsumoto, Tatsuya Masuda, Yuji Takahashi, Takahiro Katsuki, Kenji Kai, Nobuaki Shiraki, Hiroshi Inui, Ryoichi Yamaji
A deficient pancreatic β-cell mass increases the risk of type 2 diabetes mellitus. Here, we investigated the effects of testosterone on the development of pancreatic β-cell mass in male rats. The β-cell mass of male rats castrated at 6 weeks of age was reduced to ~30% of that of control rats at 16 weeks of age, and castration caused glucose intolerance. Loss of β-cell mass occurred due to decreases in islet density/pancreas and β-cell cluster size. Castration was negatively associated with the number of Ki67-positive β-cells and positively associated with the number of TUNEL-positive β-cells...
November 14, 2017: American Journal of Physiology. Endocrinology and Metabolism
https://www.readbyqxmd.com/read/29129208/augmented-gene-expression-triggered-by-na-k-atpase-inhibition-role-of-ca-2-i-mediated-and-independent-excitation-transcription-coupling
#20
Larisa V Smolyaninova, Svetlana V Koltsova, Svetlana V Sidorenko, Sergei N Orlov
In rat vascular smooth muscle cells (RVSMC), 3-h Na(+),K(+)-ATPase inhibition by ouabain or in K(+)-free medium resulted in the inversion of the [Na(+)]i/[K(+)]i ratio and elevation up to 7-fold the content of Egr1, Atf3, Nr4a1 and Ptgs2 mRNAs. Ouabain increased the rate of 45Ca(2+) influx by 2-fold that was abolished by L-type voltage-gated Ca(2+) channel blocker nicardipine, but it was resistant to Na(+)/Ca(2+) exchanger inhibitor KB-R7943. To study the role of Ca(2+)-mediated signaling in the expression of Na(+)i/K(+)i-sensitive genes we used intracellular Ca(2+) chelator BAPTA and incubated RVSMC in Ca(2+)-free medium...
December 2017: Cell Calcium
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