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https://www.readbyqxmd.com/read/29054015/synergistic-effect-of-copper-and-arsenic-upon-oxidative-stress-inflammation-and-autophagy-alterations-in-brain-tissues-of-gallus-gallus
#1
Xiao Sun, Jinglun Li, Hongjing Zhao, Yu Wang, Juanjuan Liu, Yizhi Shao, Yuan Xue, Mingwei Xing
Arsenic or copper is one of the most highly toxic pollution that can cause dysfunction to brains, however, the exact mechanism remains unclear. The aim of the study is to investigate the mechanisms of arsenic or/and copper-induced oxidative stress, inflammation and autophagy in chicken brains and elucidate the interactions between arsenic and copper. A total of 72 1-day-old Hy-line chickens were divided into four groups (18 chickens per group) treated with 30mg/kg arsenic trioxide (As2O3) or/and 300mg/kg copper sulfate (CuSO4) for 12weeks...
October 12, 2017: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/29053565/a-synthetic-analogue-of-neopeltolide-8-9-dehydroneopeltolide-is-a-potent-anti-austerity-agent-against-starved-tumor-cells
#2
Haruhiko Fuwa, Mizuho Sato
Neopeltolide, an antiproliferative marine macrolide, is known to specifically inhibit complex III of the mitochondrial electron transport chain (mETC). However, details of the biological mode-of-action(s) remain largely unknown. This work demonstrates potent cytotoxic activity of synthetic neopeltolide analogue, 8,9-dehydroneopeltolide (8,9-DNP), against starved human pancreatic adenocarcinoma PANC-1 cells and human non-small cell lung adenocarcinoma A549 cells. 8,9-DNP induced rapid dissipation of the mitochondrial membrane potential and depletion of intracellular ATP level in nutrient-deprived medium...
October 20, 2017: Marine Drugs
https://www.readbyqxmd.com/read/29052919/oamdp-a-novel-podophyllotoxin-derivative-induces-apoptosis-cell-cycle-arrest-and-autophagy-in-hepatoma-hepg2-cells
#3
Jie Ren, Yu Liu, Lixia Li, Yuexin Zhao, Zhongyu Li, Chao Wu, Lin Chen, Kun Hu
4β-(1,3,4-oxadiazole-2-amino-5-methyl)-4-deoxypodophyllotoxin (OAMDP), a novel podophyllotoxin derivative, has demonstrated potent anti-tumor activity with significant cytotoxic effect. Here we report the anti-proliferative effect of OAMDP, for which OAMDP could suppress the proliferation of HepG2 cells (human hepatoma cell line) in a dose- and time-dependent manner. After treating with OAMDP, cell apoptosis was confirmed by Annexin V-FITC/PI double staining assay. Furthermore, flow cytometry analysis revealed the loss of mitochondrial membrane potential and the increase of intracellular reactive oxygen species in HepG2 cells...
October 20, 2017: Cell Biology International
https://www.readbyqxmd.com/read/29052400/-protective-effect-of-ginsenoside-rb%C3%A2-on-doxorubicin-induced-myocardial-autophagy
#4
Long-Fei Li, Zeng-Chun Ma, Yu-Guang Wang, Xiang-Lin Tang, Hong-Ling Tan, Cheng-Rong Xiao, Yue Gao
Ginsenoside Rb₁ (Rb₁), which is one of the main ingredients derived from Panax ginseng, has been found to have extensive pharmacological activities including antioxidant, anti-inflammatory, anticancer properties. In this study, the effect of Rb₁ on doxorubicin-induced myocardial autophagy was studied with H9c2 as the study object. CCK-8 method, transmission electron microscope observation, fluorescence staining observation and Western blot were used to detect changes in H9c2 cell proliferation and autophagy after treatment...
April 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29052399/-effect-of-total-flavonoids-in-scutellaria-barbata-in-mediating-autophagy-in-tumor-cells-via-pi3k-akt-mtor-pathway
#5
Ming Chen, Ju-Tao Wang, Zhen-Ni Wu, Man-Yan Hu, Hua-Wu Gao
To investigate the effect of the total flavonoids in Scutellaria barbata(TF-SB) against autophagy in tumor cells in vivo, and further determine whether the mechanism is correlated with the PI3K/AKT/mTOR pathway, which lead to autophagy-induced tumor cell death. Melanoma-bearing mice were prepared and divided into control group, rapamycin group (Rap 1.5 mg•kg⁻¹), and high, middle and low-dose TF-SB (200, 100, 50 mg•kg⁻¹) groups. The groups were given drugs once a day for successively 11 days. The inhibitory effect of TF-SB on the growth of melanoma was determined by measuring tumor volume and tumor inhibition rate...
April 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29050275/alisol-b-23-acetate-induces-autophagic-dependent-apoptosis-in-human-colon-cancer-cells-via-ros-generation-and-jnk-activation
#6
Yueliang Zhao, Edmund T S Li, Mingfu Wang
Alisol B 23-acetate (AB23A), a natural triterpenoid from the rhizome of Alisma orientale, a Chinese medicinal herb, has multiple physiological activities including anticancer. However, its effect on human colon cancer and the underlying mechanism are not clear. Here, we reported for the first time that AB23A induced cell cycle G1 phase arrest and apoptotic cell death in colon cancer cells. Autophagy also occurred in AB23A-treated HCT116 cells as evidenced by the accumulation of microtubule-associated protein 1 light chain 3 form II (LC3-II) and degradation of SQSTM1/p62...
September 19, 2017: Oncotarget
https://www.readbyqxmd.com/read/29050003/cantharidin-inhibits-the-growth-of-triple-negative-breast-cancer-cells-by-suppressing-autophagy-and-inducing-apoptosis-in-vitro-and-in-vivo
#7
Hong-Chang Li, Zhi-Hua Xia, Ya-Feng Chen, Fan Yang, Wen Feng, Han Cai, Yi Mei, Yi-Ming Jiang, Ke Xu, Dian-Xu Feng
BACKGROUND/AIMS: Cantharidin, a type of terpenoid secreted by the blister beetle Mylabris phalerata (Pallas), has attracted great attention in cancer therapy because of its potential anti-cancer activities. Here, we report the effects on apoptosis and autophagy in human triple-negative breast cancer (TNBC) cell lines after treatment with cantharidin and attempt to elucidate the underlying mechanisms. METHODS: MDA-MB-231 and MDA-MB-468 cells were treated with cantharidin and cell proliferation was examined using CCK-8 and clone formation assays...
October 19, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29049987/isoflavones-induce-bex2-dependent-autophagy-to-prevent-atr-induced-neurotoxicity-in-sh-sy5y-cells
#8
Peng Li, Kun Ma, Hao-Yu Wu, Yan-Ping Wu, Bai-Xiang Li
BACKGROUND/AIMS: Atrazine (ATR) is a broad-spectrum herbicide in wide use around the world. However, ATR is neurotoxic and can cause cell death in dopaminergic neurons, leading to neurodegenerative disorders. Autophagy is the basic cellular catabolic process involving the degradation of proteins and damaged organelles. Studies have shown that certain plant compounds can induce autophagy and prevent neuronal cell death. This prompted us to investigate plant compounds that might reduce the neurotoxic effects of ATR...
October 19, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29048631/autophagy-induction-enhances-tetrandrine-induced-apoptosis-via-the-ampk-mtor-pathway-in-human-bladder-cancer-cells
#9
Bo Kou, Wei Liu, Xu Xu, Yang Yang, Qiuyue Yi, Fengwei Guo, Jianpeng Li, Jinsong Zhou, Qingshan Kou
Tetrandrine, a bisbenzylisoquinoline alkaloid isolated from the roots of Stephania tetrandra is a traditional Chinese medicine and exerts anticancer capacity in various types of cancers. Previous studies have shown that tetrandrine induces apoptosis in bladder cancer cells via activation of the caspase cascade. However, the underlying mechanism has not yet been reported. Autophagy is a cellular process involved in the degradation of broken proteins and aging organelles to maintain homeostasis. Recent studies indicate that autophagy is implicated in cancer therapy...
September 21, 2017: Oncology Reports
https://www.readbyqxmd.com/read/29047155/maduramicin-induces-apoptosis-and-necrosis-and-blocks-autophagic-flux-in-myocardial-h9c2-cells
#10
Xin Chen, Long Chen, Shanxiang Jiang, Shile Huang
Maduramicin, a polyether ionophore antibiotic, is widely used as an anticoccidial agent in the poultry industry. It has been reported that maduramicin may cause heart and skeletal muscle cell damage, resulting in heart failure, skeletal muscle degeneration and even death in animals and humans, if improperly used. However, the molecular mechanism behind its capability to cause death of cardiac cells is not known. Here, we show that maduramicin induced apoptosis and necrosis in rat myocardial cells (H9c2). Maduramicin did not apparently upregulate the expression of pro-apoptotic proteins (e...
October 19, 2017: Journal of Applied Toxicology: JAT
https://www.readbyqxmd.com/read/29046869/staphylococcus-aureus-alpha-toxin-induces-the-formation-of-dynamic-tubules-labeled-with-lc3-within-host-cells-in-a-rab7-and-rab1b-dependent-manner
#11
María M López de Armentia, María C Gauron, María I Colombo
Staphylococcus aureus is a pathogen that causes severe infectious diseases that eventually lead to septic and toxic shock. S. aureus infection is characterized by the production of virulence factors, including enzymes and toxins. After internalization S. aureus resides in a phagosome labeled with Rab7 protein. Here, we show that S. aureus generates tubular structures marked with the small GTPases Rab1b and Rab7 and by the autophagic protein LC3 at early times post-infection. As shown by live cell imaging these tubular structures are highly dynamic, extend, branch and grow in length...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/29046293/exercise-induces-tfeb-expression-and-activity-in-skeletal-muscle-in-a-pgc-1%C3%AE-dependent-manner
#12
Avigail T Erlich, Diane M Brownlee, Kaitlyn Beyfuss, David A Hood
The mitochondrial network in muscle is controlled by the opposing processes of mitochondrial biogenesis and mitophagy. The coactivator PGC-1α regulates biogenesis, while the transcription of mitophagy-related genes is controlled by transcription factor EB (TFEB). PGC-1α activation is induced with exercise, however the effect of exercise on TFEB is not fully known. We investigated the interplay between PGC-1α and TFEB on mitochondria in response to acute contractile activity in C2C12 myotubes, and following exercise in WT and PGC-1α KO mice...
October 18, 2017: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/29042965/overexpression-of-let-7a-increases-neurotoxicity-in-a-pc12-cell-model-of-alzheimer-s-disease-via-regulating-autophagy
#13
Huizi Gu, Lan Li, Chen Cui, Zihui Zhao, Guijun Song
Increased deposition of β-amyloid (Aβ) protein is one of the typical characteristics of Alzheimer's disease (AD). Recent evidence has demonstrated that the microRNA let-7 family, which is highly expressed in the central nervous system, participates in the regulation of pathologic processes of AD. In the present study, the effect of let-7a overexpression on Aβ1-40-induced neurotoxicity was evaluated in PC12 and SK-N-SH cells. The results indicated that overexpression of let-7a enhanced the neurotoxicity induced by Aβ1-40 in PC12 and SK-N-SH cells...
October 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29042950/expression-of-ccr3-sox5-and-lc3-in-patients-with-elderly-onset-rheumatoid-arthritis-and-the-clinical-significance
#14
Jing Wang, Qing Zhao
This study investigated the expression of C-C chemokine receptor type 3 (CCR3), transcription factor SOX5 (SOX5) and microtubule-associated protein 1 light chain 3 (LC3) in patients with elderly onset rheumatoid arthritis (EORA) and the clinical significance. Ninety patients with elderly onset rheumatoid arthritis were selected in our hospital from January to December in 2016 to serve as patient group. At the same time, 50 healthy people were selected as control group. Levels of CCR3, SOX5 and LC3 in serum of two groups were detected by enzyme-linked immunosorbent assay (ELISA)...
October 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29039784/crosstalk-influence-between-p38mapk-and-autophagy-on-mitochondria-mediated-apoptosis-induced-by-anti-fas-antibody-actinomycin-d-in-human-hepatoma-bel-7402-cells
#15
Yu Wang, Chunhui Xia, Yanxin Lv, Chengjun Li, Qingbu Mei, Hongmei Li, Haijun Wang, Shuang Li
Our previous study indicated that anti-Fas antibody/actinomycin D (AF/AD) induced apoptosis of human hepatocellular carcinoma Bel-7402 cells; however, crosstalk influence between P38MAPK and autophagy on mitochondria-mediated apoptosis induced by AF/AD in Bel-7402 cells remains unclear. Therefore, effect of AF/AD on apoptosis, autophagy, phosphorylated-P38MAPK (p-P38MAPK), and membrane potential (ΔΨm) with or without the P38MAPK inhibitor SB203580 or the autophagy inhibitor 3-methyladenine (3-MA) in Bel-7402 cells was investigated in the present study...
October 17, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29039462/antiproliferative-activity-of-di-2-pyridylhydrazone-dithiocarbamate-acetate-partly-involved-in-p53-mediated-apoptosis-and-autophagy
#16
Tingting Wang, Youxun Liu, Yun Fu, Tengfei Huang, Yun Yang, Shaoshan Li, Changzheng Li
Cancer cells have higher demand of iron and copper ions for growth, disturbing the metal's homeostasis can inhibit proliferation of cancer cell. Dithiocarbamates possessing excellent metal chelating ability and antitumor activity are considered as candidates in chelation therapy, however, their antitumor molecular mechanisms remain to be elucidated. In the present study, a dithiocarbamate derivative, di-2-pyridylhydrazone dithiocarbamate s-acetic acid (DpdtaA) was prepared to address the issue whether the molecular mechanism behind biological behavior showed by dithiocarbamate was p53 mediated...
October 10, 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/29039105/autophagy-monitoring-assay-ii-imaging-autophagy-induction-in-llc-pk1-cells-using-gfp-lc3-protein-fusion-construct
#17
Pavan P Adiseshaiah, Sarah L Skoczen, Jamie C Rodriguez, Timothy M Potter, Krishna Kota, Stephan T Stern
Autophagy is a catabolic process involved in the degradation and recycling of long-lived proteins and damaged organelles for maintenance of cellular homeostasis, and it has also been proposed as a type II cell death pathway. The cytoplasmic components targeted for catabolism are enclosed in a double-membrane autophagosome that merges with lysosomes, to form autophagosomes, and are finally degraded by lysosomal enzymes. There is substantial evidence that several nanomaterials can cause autophagy and lysosomal dysfunction, either by prevention of autophagolysosome formation, biopersistence or inhibition of lysosomal enzymes...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29038162/systematic-analysis-of-human-cells-lacking-atg8-proteins-uncovers-roles-for-gabaraps-and-the-ccz1-mon1-regulator-c18orf8-rmc1-in-macro-and-selective-autophagic-flux
#18
Laura Pontano Vaites, Joao A Paulo, Edward L Huttlin, J Wade Harper
Selective and macro autophagy sequester specific organelles/substrates or bulk cytoplasm, respectively, inside autophagosomes as cargo for delivery to lysosomes. The mammalian ATG8 orthologues (MAP1LC3A/B/C and GABARAP/L1/L2) are ubiquitin (UB)-like proteins conjugated to the autophagosome membrane and are thought to facilitate cargo receptor recruitment, vesicle maturation, and lysosomal fusion. To elucidate the molecular functions of the ATG8 proteins, we engineered cells lacking genes for each subfamily as well as all six mammalian ATG8s...
October 16, 2017: Molecular and Cellular Biology
https://www.readbyqxmd.com/read/29036763/effect-of-treadmill-exercise-on-skeletal-muscle-autophagy-in-rats-with-obesity-induced-by-a-high-fat-diet
#19
Do Keun Cho, Dong Hun Choi, Joon Yong Cho
PURPOSE: This study aimed to investigate the effects of treadmill exercise on body weight, blood biochemistry, and autophagy. METHODS: Triglyceride, total cholesterol, low-density lipoprotein cholesterol, high-density lipoprotein cholesterol, insulin, and glucose levels were measured, the Homeostatic Model Assessment of Insulin Resistance (HOMA-IR) score was calculated, and the soleus muscle was analyzed for autophagy-related factors (Beclin-1, p62, LC3, Lamp-2) in rats with obesity induced by a high-fat diet...
September 30, 2017: Journal of Exercise Nutrition & Biochemistry
https://www.readbyqxmd.com/read/29036449/resveratrol-attenuates-bone-cancer-pain-through-regulating-the-expression-levels-of-asic3-and-activating-cell-autophagy
#20
Haili Zhu, Jieqiong Ding, J Wu, Tingting Liu, Jing Liang, Qiong Tang, Ming Jiao
Bone cancer pain (BCP) is one of the most common pains in patients with malignant cancers. The mechanism underlying BCP is largely unknown. Our previous studies and the increasing evidence both have shown that acid-sensing ion channels 3 (ASIC3) is an important protein in the pathological pain state in some pain models. We hypothesized that the expression change of ASIC3 might be one of the factors related to BCP. In this study, we established the BCP model through intrathecally injecting rat mammary gland carcinoma cells (MRMT-1) into the left tibia of Sprague-Dawley female rats, and found that the BCP rats showed bone destruction, increased mechanical pain sensitivities and up-regulated ASIC3 protein expression levels in L4-L6 dorsal root ganglion...
October 3, 2017: Acta Biochimica et Biophysica Sinica
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