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Paromita Sarbadhikary, Alok Dube
We investigated the anticancer chemotoxicity of previously reported iodinated chlorin p6 copper complex (ICp6-Cu), a novel chlorophyll derivative in which copper is attached to the side chain carboxylate groups via coordination. Human oral carcinoma cells NT8e, 4451 and the non-cancerous keratinocyte HaCaT cells were treated with ICp6-Cu for 48 h in dark and cell viability, proliferation and morphological alterations were examined. ICp6-Cu showed pronounced cytotoxicity in cancer cells with IC50 ∼40 μM, whereas, the viability of HaCaT cells was not affected...
September 13, 2017: Chemico-biological Interactions
Ria de Haas, Devashish Das, Alejandro Garanto, Herma G Renkema, Rick Greupink, Petra van den Broek, Jeanne Pertijs, Rob W J Collin, Peter Willems, Julien Beyrath, Arend Heerschap, Frans G Russel, Jan A Smeitink
Leigh Disease is a progressive neurometabolic disorder for which a clinical effective treatment is currently still lacking. Here, we report on the therapeutic efficacy of KH176, a new chemical entity derivative of Trolox, in Ndufs4 (-/-) mice, a mammalian model for Leigh Disease. Using in vivo brain diffusion tensor imaging, we show a loss of brain microstructural coherence in Ndufs4 (-/-) mice in the cerebral cortex, external capsule and cerebral peduncle. These findings are in line with the white matter diffusivity changes described in mitochondrial disease patients...
September 15, 2017: Scientific Reports
Ivan Ivanov, Nadezhda Petkova, Julian Tumbarski, Ivayla Dincheva, Ilian Badjakov, Panteley Denev, Atanas Pavlov
A comparative investigation of n-hexane soluble compounds from aerial parts of dandelion (Taraxacum officinale Weber ex F.H. Wigg.) collected during different vegetative stages was carried out. The GC-MS analysis of the n-hexane (unpolar) fraction showed the presence of 30 biologically active compounds. Phytol [14.7% of total ion current (TIC)], lupeol (14.5% of TIC), taraxasteryl acetate (11.4% of TIC), β-sitosterol (10.3% of TIC), α-amyrin (9.0% of TIC), β-amyrin (8.3% of TIC), and cycloartenol acetate (5...
September 13, 2017: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
Diego Archaina, Graciela Leiva, Daniela Salvatori, Carolina Schebor
Blackcurrants contain high levels of polyphenolics, particularly flavonols and anthocyanins, which contribute to their high antioxidant activity. The aims of this work were the recovery of bioactive compounds from the remaining solid (waste) after processing blackcurrant juice and to obtain spray-dried powders from the blackcurrant juice and extracts. The extraction of bioactive compounds from the fruit pulp was performed by ultrasound-assisted extraction. Experiments were conducted to select the more suitable solvent, and citric acid was chosen...
January 1, 2017: Food Science and Technology International, Ciencia y Tecnología de Los Alimentos Internacional
Bibo Wang, Jing Fu, Ting Yu, An Xu, Wenhao Qin, Zhishi Yang, Yao Chen, Hongyang Wang
Conflicting effects of antioxidant supplementation on cancer prevention or promotion is of great concern to healthy people and cancer patients. Despite recent studies about antioxidants accelerating the progression of lung cancer and melanoma, it does not fully deny antioxidants for cancer prevention. Both tumor and antioxidants types influence the actual efficacy. However, little is known about the impact of different types of antioxidants on primary hepatocellular carcinoma (HCC), including non-mitochondrial- and mitochondrial-targeted antioxidants...
September 12, 2017: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
Dörthe F Bebensee, Karolina Can, Michael Müller
Rett syndrome (RTT) is a neurodevelopmental disorder with mutations in the MECP2 gene. Mostly girls are affected, and an apparently normal development is followed by cognitive impairment, motor dysfunction, epilepsy, and irregular breathing. Various indications suggest mitochondrial dysfunction. In Rett mice, brain ATP levels are reduced, mitochondria are leaking protons, and respiratory complexes are dysregulated. Furthermore, we found in MeCP2-deficient mouse (Mecp2(-/y) ) hippocampus an intensified mitochondrial metabolism and ROS generation...
2017: Oxidative Medicine and Cellular Longevity
Anusha Allam, S R Ravikiran, B Shantaram Baliga, Kamalakshi Bhat, Nitin Joseph
OBJECTIVE: To compare oxidative stress due to conventional and LED phototherapy among jaundiced preterm neonates. METHODS: Cross-sectional study conducted in NICU on 82 neonates (equal numbers received conventional and LED phototherapy). Total antioxidant capacity (TAC), total oxidant status (TOS) and Oxidative stress index (OSI) were assessed. RESULTS: Post-phototherapy, mean (SD) OSI increased significantly compared to baseline in both conventional [0...
August 15, 2017: Indian Pediatrics
Prashant Sakharkar, Balwantsinh Chauhan
OBJECTIVE: Little knowledge is available on the antimicrobial and antioxidant properties of Coccina grandis fruits and no study has reported on its cell proliferative property. The aim of this study was to examine the antimicrobial, antioxidant and cell proliferative property of fruits of C. grandis. MATERIALS AND METHODS: Fruits of C. grandis were extracted using water; ethanol and acetone by cold and hot Soxhlet extraction. The antibacterial activities of the extracts were tested against Staphylococcusaureus,Enterococcusfaecalis,Escherichiacoli and Pseudomonasaeruginosa using the modified Kirby-Bauer diffusion method and compared against erythromycin...
July 2017: Avicenna Journal of Phytomedicine
Ya Li, Sha Li, Sheng-Jun Lin, Jiao-Jiao Zhang, Cai-Ning Zhao, Hua-Bin Li
Our previous study reported that the fruit of Gordonia axillaris , an edible wild fruit, possessed strong antioxidant activity. In this study, a microwave-assisted extraction (MAE) method was established to extract antioxidants from the fruit of Gordonia axillaris . The influence of five parameters, including ethanol concentration, solvent/material ratio, extraction time, extraction temperature and microwave power, was investigated by single-factor experiments. Three factors, namely ethanol concentration, solvent/material ratio, extraction time, were found to exert a major influence on extraction efficacy, and were further studied by response surface methodology to investigate their interactions...
September 6, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Chao Chen, Li Wang, Ren Wang, Xiaohu Luo, Yongfu Li, Juan Li, Yanan Li, Zhengxing Chen
The objectives of this research were to determine the phenolic contents, oxygen radical absorbance capacities (ORAC), cellular antioxidant activities (CAA), and antiproliferative capacities of nine oat varieties and four brans in China. Of all varieties, Longyan 3 and Beiyan 1 exhibited the highest total avenanthramides (146.94±7.31 and 120.95±6.66µg/g, respectively) and ORAC values (21.03±0.56 and 21.18±1.45µM Trolox/g, respectively), while Shaotong exhibited the highest total phenolic acids (143.52±9...
January 15, 2018: Food Chemistry
Arivalagan Manivannan, Rakesh Bhardwaj, Sugatha Padmanabhan, Poonam Suneja, K B Hebbar, Santosh R Kanade
Study was conducted to determine the biochemical constituents in coconut (Cocos nucifera L.) haustorium, a spongy tissue formed during coconut germination. Results indicated that 100g of dried coconut haustorium contained 1.05±0.2% ash, 44.2±4.6% soluble sugar, 24.5±3.2% starch, 5.50±0.3% protein, 1.99±0.9% fat, 5.72±0.4% soluble dietary fibre, 20.3±1.9% insoluble dietary fibre, and 146±14.3mg phenolics. Mineral profiling showed that it contained 145±8.6, 104±9.6, 33.9±8.2, 30.9±1.9, 9.45±2.1, 0...
January 1, 2018: Food Chemistry
Gaurav Rajauria, Barry Foley, Nissreen Abu-Ghannam
This study explored Himanthalia elongata brown seaweed as a potential source of dietary fucoxanthin which is a promising medicinal and nutritional ingredient. The seaweed was extracted with low polarity solvents (n-hexane, diethyl ether, and chloroform) and the crude extract was purified with preparative thin layer chromatography (P-TLC). Identification, quantification and structure elucidation of purified compounds was performed by LC-DAD-ESI-MS and NMR ((1)H and (13)C). P-TLC led purification yielded 18.6mg/g fucoxanthin with 97% of purity based on the calibration curve, in single-step purification...
September 2017: Food Research International
Dillon Lee, Fatemeh Bamdad, Kevin Khey, Hoon H Sunwoo
Vitelline membrane (VM) is a multilayered structure that surrounds the egg yolk serving to separate the yolk and the white. Due to its poor solubility in aqueous-based media, VM proteins and their biological properties have not been fully defined. In the current study, VM was hydrolyzed using different enzymes under the optimum hydrolysis conditions. Antioxidant and anti-inflammatory properties were evaluated in chemical and cellular models. Flavourzyme- and trypsin-treated samples showed the highest radical scavenging and ferric ion reducing effect (31% and 20 μM of Trolox equivalents/mg, respectively)...
September 1, 2017: Poultry Science
Viktorija Glembockyte, Gonzalo Cosa
Here we report transient absorption studies on the ground-state recovery dynamics of the single-molecule fluorophore Cy3B in the presence of four different photostabilizing agents, namely β-mercaptoethanol (β-ME), Trolox (TX), n-propyl gallate (n-PG), and ascorbic acid (AA). These are triplet-state quenchers that operate via photoinduced electron transfer (PeT). While quantitative geminate recombination was recorded following PeT for β-ME (∼100%), for Trolox, n-propyl gallate, and ascorbic acid the extent of geminate recombination was >48%, >27%, and >13%, respectively...
September 8, 2017: Journal of the American Chemical Society
Sabiha Runa, Melike Lakadamyali, Melissa L Kemp, Christine K Payne
Titanium dioxide (TiO2) nanoparticles, used as pigments and photocatalysts, are widely present in modern society. Inhalation or ingestion of these nanoparticles can lead to cellular-level interactions. We examined the very first step in this cellular interaction, the effect of TiO2 nanoparticles on the lipids of the plasma membrane. Within 12 h of TiO2 nanoparticle exposure, the lipids of the plasma membrane were oxidized, determined with a malondialdehyde assay. Lipid peroxidation was inhibited by surface passivation of the TiO2 nanoparticles, incubation with an antioxidant (Trolox), and the presence of serum proteins in solution...
September 11, 2017: Journal of Physical Chemistry. B
Maria Neginskaya, Elena Berezhnaya, Anatoly B Uzdensky, Andrey Y Abramov
Photodynamic therapy (PDT) leads to production of reactive oxygen species (ROS) and cell destruction due to oxidative stress. We used photodynamic effect of photosensitizer radachlorin to unravel the effect of photo-induced oxidative stress on the calcium signal and lipid peroxidation in primary culture of cortical neurons and astrocytes using live cell imaging. We have found that irradiation in presence of 200 nM of radachlorin induces calcium signal in primary neurons and astrocytes. Photo-induced neuronal calcium signal depends on internal calcium stores as it was still observed in calcium-free medium and could be blocked by depletion of endoplasmic reticulum (ER) stores with inhibitor of sarco-endoplasmic reticulum Ca(2+) ATPase (SERCA) thapsigargin...
August 26, 2017: Molecular Neurobiology
Yamile A Mennah-Govela, Gail M Bornhorst
This study aims to analyze the influence of processing and orange variety on initial quality, antioxidant activity, total polyphenol content (TPC), and ascorbic acid content of fresh-squeezed orange juice during in vitro digestion. Fresh-squeezed orange juice was made from Fukumoto Navel, Lane Late Navel, Olinda Nucellar Valencia, and Campbell Valencia oranges, and was processed thermally and nonthermally. Antioxidant activity (FRAP and ABTS assays), TPC (Folin-Ciocalteu method), and ascorbic acid were analyzed before and after gastrointestinal digestion...
August 23, 2017: Journal of Food Science
L F Jorge, A B Meniqueti, R F Silva, K A Santos, E A Da Silva, J E Gonçalves, C M De Rezende, N B Colauto, Z C Gazim, G A Linde
In this study, the temperature and pressure of supercritical CO2 extraction were evaluated to obtain oleoresin of Brunfelsia uniflora leaves and flowers. The oleoresin compounds were identified by gas chromatography-mass spectrometry. The antioxidant activity was evaluated by three different methods. The highest oleoresin yields were 3.32% at 40°C and 200 bar for the leaves, and 1.03% at 60°C and 200 bar for the flowers. The main extracted compounds from leaves were phytol varying from 11.95 to 36.42% and α-tocopherol from 15...
August 17, 2017: Genetics and Molecular Research: GMR
Ivana Zrnzevic, Miroslava Stankovic, Vesna Stankov Jovanovic, Violeta Mitic, Aleksandra Dordevic, Ivana Zlatanovic, Gordana Stojanovic
In the present investigation, effects of Ramalina capitata acetone extract on micronucleus distribution on human lymphocytes, on cholinesterase activity and antioxidant activity (by the CUPRAC method) were examined, for the first time as well as its HPLC profile. Additionally, total phenolic compounds (TPC), antioxidant properties (estimated via DPPH, ABTS and TRP assays) and antibacterial activity were determined. The predominant phenolic compounds in this extract were evernic, everninic and obtusatic acids...
2017: EXCLI journal
Sezin Unal, Nihal Demirel, Deniz Yaprak Sul, Dilek Ulubas Isik, Sara Erol, Salim Neselioglu, Ozcan Erel, Ahmet Yagmur Bas
OBJECTIVE: There exists evidence that phototherapy can disturb the oxidant/antioxidant balance in favor of oxidants. If phototherapy is continued during tube feeding in preterms, human milk is subjected to phototherapy lights for about 20 minutes per feeding. We aimed to investigate the effects of phototherapy lights on oxidative/antioxidative status of human milk. STUDY DESIGN: Milk samples of 50 healthy mothers were grouped as control and phototherapy and exposed to 20 minutes of day-light and phototherapy light respectively...
August 21, 2017: Journal of Maternal-fetal & Neonatal Medicine
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