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https://www.readbyqxmd.com/read/28725659/explant-methodology-for-analyzing-neuroblast-migration
#1
Kirsty J Dixon, Alisa Turbic, Ann M Turnley, Daniel J Liebl
The subventricular zone (SVZ) in the mammalian forebrain contains stem/progenitor cells that migrate through the rostral migratory stream (RMS) to the olfactory bulb throughout adulthood. SVZ-derived explant cultures provide a convenient method to assess factors regulating the intermediary stage of neural stem/progenitor cell migration. Here, we describe the isolation of SVZ-derived RMS explants from the neonatal mouse brain, and the conditions required to culture and evaluate their migration.
May 5, 2017: Bio-protocol
https://www.readbyqxmd.com/read/28725182/canonical-and-novel-non-canonical-cholinergic-agonists-inhibit-atp-induced-release-of-monocytic-interleukin-1%C3%AE-via-different-combinations-of-nicotinic-acetylcholine-receptor-subunits-%C3%AE-7-%C3%AE-9-and-%C3%AE-10
#2
Anna Zakrzewicz, Katrin Richter, Alisa Agné, Sigrid Wilker, Kathrin Siebers, Bijan Fink, Gabriela Krasteva-Christ, Mike Althaus, Winfried Padberg, Arik J Hone, J Michael McIntosh, Veronika Grau
Recently, we discovered a cholinergic mechanism that inhibits the adenosine triphosphate (ATP)-dependent release of interleukin-1β (IL-1β) by human monocytes via nicotinic acetylcholine receptors (nAChRs) composed of α7, α9 and/or α10 subunits. Furthermore, we identified phosphocholine (PC) and dipalmitoylphosphatidylcholine (DPPC) as novel nicotinic agonists that elicit metabotropic activity at monocytic nAChR. Interestingly, PC does not provoke ion channel responses at conventional nAChRs composed of subunits α9 and α10...
2017: Frontiers in Cellular Neuroscience
https://www.readbyqxmd.com/read/28720803/contingent-sounds-change-the-mental-representation-of-one-s-finger-length
#3
Ana Tajadura-Jiménez, Maria Vakali, Merle T Fairhurst, Alisa Mandrigin, Nadia Bianchi-Berthouze, Ophelia Deroy
Mental body-representations are highly plastic and can be modified after brief exposure to unexpected sensory feedback. While the role of vision, touch and proprioception in shaping body-representations has been highlighted by many studies, the auditory influences on mental body-representations remain poorly understood. Changes in body-representations by the manipulation of natural sounds produced when one's body impacts on surfaces have recently been evidenced. But will these changes also occur with non-naturalistic sounds, which provide no information about the impact produced by or on the body? Drawing on the well-documented capacity of dynamic changes in pitch to elicit impressions of motion along the vertical plane and of changes in object size, we asked participants to pull on their right index fingertip with their left hand while they were presented with brief sounds of rising, falling or constant pitches, and in the absence of visual information of their hands...
July 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28712357/medicare-accountable-care-organizations-and-antidepressant-use-by-patients-with-depression
#4
Alisa B Busch, Haiden A Huskamp, Amanda R Kreider, J Michael McWilliams
OBJECTIVE: This study examined whether Medicare accountable care organization (ACO) programs were associated with early changes in antidepressant use or adherence among beneficiaries with depression. METHODS: A difference-in-difference design was used to compare claims from Medicare fee-for-service beneficiaries (2009-2013) and ACO patients with those from local control groups. Outcome measures were total antidepressant days supplied, filling one or more antidepressant prescriptions, and proportion of days covered (PDC) by supply among antidepressant users (adherence)...
July 17, 2017: Psychiatric Services: a Journal of the American Psychiatric Association
https://www.readbyqxmd.com/read/28700631/is-the-level-of-knowledge-a-predictor-of-rational-antibiotic-use-in-serbia
#5
Olga J Horvat, Ana D Tomas, Milica M Paut Kusturica, Alisa V Savkov, Dragica U Bukumirić, Zdenko S Tomić, Ana J Sabo
INTRODUCTION: The objective of this study was to investigate the knowledge, attitudes and behavior regarding antibiotics of the general population. METHODS: The study sample consisted of adult subjects who consulted general practitioners at health centers in Serbia and agreed to complete the questionnaire. A total of 668 questionnaires were distributed; 500 respondents completed the entire questionnaire (response rate 74.85%). RESULTS: The average age was 51...
2017: PloS One
https://www.readbyqxmd.com/read/28689138/enhanced-dna-double-strand-break-repair-of-microbeam-targeted-a549-lung-carcinoma-cells-by-adjacent-wi38-normal-lung-fibroblast-cells-via-bi-directional-signaling
#6
Alisa Kobayashi, Tengku Ahbrizal Farizal Tengku Ahmad, Narongchai Autsavapromporn, Masakazu Oikawa, Shino Homma-Takeda, Yoshiya Furusawa, Jun Wang, Teruaki Konishi
Understanding the mechanisms underlying the radiation-induced bystander effect (RIBE) and bi-directional signaling between irradiated carcinoma cells and their surrounding non-irradiated normal cells is relevant to cancer radiotherapy. The present study investigated propagation of RIBE signals between human lung carcinoma A549 cells and normal lung fibroblast WI38 cells in bystander cells, either directly or indirectly contacting irradiated A549 cells. We prepared A549-GFP/WI38 co-cultures and A549-GFP/A549 co-cultures, in which A549-GFP cells stably expressing H2BGFP were co-cultured with either A549 cells or WI38 cells, respectively...
June 23, 2017: Mutation Research
https://www.readbyqxmd.com/read/28688569/assessment-of-the-feeding-swallowing-impact-survey-as-a-quality-of-life-measure-in-children-with-laryngeal-cleft-before-and-after-repair
#7
M Shannon Fracchia, Gillian Diercks, Alisa Yamasaki, Cheryl Hersh, Stephen Hardy, Marina Hartnick, Christopher Hartnick
OBJECTIVE: To cross-validate the Feeding Swallowing Impact Survey (FSIS), a quality of life instrument, specifically to a subpopulation of children who aspirate due to laryngeal cleft. INTRODUCTION: The FSIS is a recently validated instrument used to describe caregiver quality of life (QOL) in children with aspiration due to various causes. To cross-validate the FSIS specifically to the subpopulation of children who aspirate due to laryngeal cleft, we tested the hypotheses that caregivers would report significant different scores form baseline if their children improved at the one year mark postintervention due to either successful conservative or surgical measures (discriminant validity) and would not report significant differences in their FSIS reporting if there was no change in their child's aspiration at the one year mark post intervention (convergent validity)...
August 2017: International Journal of Pediatric Otorhinolaryngology
https://www.readbyqxmd.com/read/28682250/effects-of-bi-zn2-3nb1-3-o3-modification-on-the-relaxor-behavior-and-piezoelectricity-of-pb-mg1-3nb2-3-o3-pbtio3-ceramics
#8
Zenghui Liu, Hua Wu, Alisa Paterson, Wei Ren, Zuo-Guang Ye
Relaxor lead magnesium niobate (PMN)-based materials exhibit complex structures and unusual properties that have been puzzling researchers for decades. In this work, a new ternary solid solution of Pb(Mg₁/₃Nb₂/₃)O₃-PbTiO₃- Bi(Zn₂/₃Nb₁/₃)O₃ (PMN-PT-BZN) is prepared in the form of ceramics, and the effects of the incorporation of BZN into the PMN-PT binary system are investigated. The crystal structure favors a pseudocubic symmetry and the relaxor properties are enhanced as the concentration of BZN increases...
June 29, 2017: IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control
https://www.readbyqxmd.com/read/28680059/gwas-follow-up-study-of-esophageal-squamous-cell-carcinoma-identifies-potential-genetic-loci-associated-with-family-history-of-upper-gastrointestinal-cancer
#9
Xin Song, Wen-Qing Li, Nan Hu, Xue Ke Zhao, Zhaoming Wang, Paula L Hyland, Tao Jiang, Guo Qiang Kong, Hua Su, Chaoyu Wang, Lemin Wang, Li Sun, Zong Min Fan, Hui Meng, Tang Juan Zhang, Ling Fen Ji, Shou Jia Hu, Wei Li Han, Min Jie Wu, Peng Yuan Zheng, Shuang Lv, Xue Min Li, Fu You Zhou, Laurie Burdett, Ti Ding, You-Lin Qiao, Jin-Hu Fan, Xiao-You Han, Carol Giffen, Margaret A Tucker, Sanford M Dawsey, Neal D Freedman, Stephen J Chanock, Christian C Abnet, Philip R Taylor, Li-Dong Wang, Alisa M Goldstein
Based on our initial genome-wide association study (GWAS) on esophageal squamous cell carcinoma (ESCC) in Han Chinese, we conducted a follow-up study to examine the single nucleotide polymorphisms (SNPs) associated with family history (FH) of upper gastrointestinal cancer (UGI) cancer in cases with ESCC. We evaluated the association between SNPs and FH of UGI cancer among ESCC cases in a stage-1 case-only analysis of the National Cancer Institute (NCI, 541 cases with FH and 1399 without FH) and Henan GWAS (493 cases with FH and 869 without FH) data (discovery phase)...
July 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28659710/fluid-shear-stress-and-fibroblast-growth-factor-2-increase-endothelial-cell-associated-vitronectin
#10
Justin G Mathew, Sarah Basehore, Alisa Morss Clyne
Vitronectin is a matricellular protein that plays an important role in both coagulation and angiogenesis through its effects on cell adhesion and the plasminogen system. Vitronectin is known to bind to endothelial cells upon integrin activation. However, the effect of integrin activation by shear stress and growth factors on cell-associated vitronectin and plasminogen system activity has not yet been studied. We therefore exposed human umbilical vein endothelial cells to steady laminar flow, oscillating disturbed flow, or fibroblast growth factor-2 (FGF-2) for 24 hours...
2017: Applied Bionics and Biomechanics
https://www.readbyqxmd.com/read/28657557/enhancing-student-empathetic-engagement-history-taking-and-communication-skills-during-electronic-medical-record-use-in-patient-care
#11
Alisa Alfonsi LoSasso, Courtney E Lamberton, Mary Sammon, Katherine T Berg, John W Caruso, Jonathan Cass, Mohammadreza Hojat
PURPOSE: To examine whether an intervention on proper use of electronic medical records (EMRs) in patient care could help improve medical students' empathic engagement, and to test the hypothesis that the training would reduce communication hurdles in clinical encounters. METHOD: Seventy third-year medical students from the Sidney Kimmel Medical College at Thomas Jefferson University were randomly divided into intervention and control groups during their six-week pediatric clerkship in 2012-2013...
July 2017: Academic Medicine: Journal of the Association of American Medical Colleges
https://www.readbyqxmd.com/read/28650397/action-observation-of-motor-skills-followed-by-immediate-sleep-enhances-the-motor-rehabilitation-of-older-adults-with-stroke
#12
Alisa A Lubart, Arthur I Leibovitz, Liora D Peleg, Alexandra I Yarovoy, Gilad O Gal, Eliyahu H Mizrahi, Emily N Lubart
BACKGROUND AND PURPOSE: Motor rehabilitation following stroke is a demanding challenge in search for new strategies to improve outcome. Rehabilitation through action observation has been reported beneficial for older adults recovering from stroke. Early sleep session following motor learning enhanced the performance of the acquired motor skills. The purpose of this study was to examine whether an early sleep session following action observation has benefit over action observation alone in the rehabilitation of older adults after stroke...
June 21, 2017: Journal of Geriatric Physical Therapy
https://www.readbyqxmd.com/read/28648065/membrane-alterations-in-pseudomonas-putida-f1-exposed-to-nanoscale-zerovalent-iron-effects-of-short-term-and-repetitive-nzvi-exposure
#13
Panaya Kotchaplai, Eakalak Khan, Alisa S Vangnai
In this study, we report the effect of the commercial nanoscale zerovalent iron (nZVI) on environmental bacteria, emphasizing the importance of nZVI-bacterial membrane interaction on nZVI toxicity as well as the adaptability of bacteria to nZVI. Exposure of Pseudomonas putida F1 to 0.1, 1.0, and 5.0 g/L of nZVI caused the reduction in colony forming units (CFUs) substantially for almost 3 orders of magnitude. However, a rebound in the cell number was observed after the prolonged exposure except for 5.0 g/L nZVI at which bacterial viability was completely inhibited...
July 10, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28639824/upside-down-perceived-space-affects-object-based-attention
#14
Frank Papenmeier, Hauke S Meyerhoff, Alisa Brockhoff, Georg Jahn, Markus Huff
Object-based attention influences the subjective metrics of surrounding space. However, does perceived space influence object-based attention, as well? We used an attentive tracking task that required sustained object-based attention while objects moved within a tracking space. We manipulated perceived space through the availability of depth cues and varied the orientation of the tracking space. When rich depth cues were available (appearance of a voluminous tracking space), the upside-down orientation of the tracking space (objects appeared to move high on a ceiling) caused a pronounced impairment of tracking performance compared with an upright orientation of the tracking space (objects appeared to move on a floor plane)...
July 2017: Journal of Experimental Psychology. Human Perception and Performance
https://www.readbyqxmd.com/read/28636944/transduction-of-repetitive-mechanical-stimuli-by-piezo1-and-piezo2-ion-channels
#15
Amanda H Lewis, Alisa F Cui, Malcolm F McDonald, Jörg Grandl
Several cell types experience repetitive mechanical stimuli, including vein endothelial cells during pulsating blood flow, inner ear hair cells upon sound exposure, and skin cells and their innervating dorsal root ganglion (DRG) neurons when sweeping across a textured surface or touching a vibrating object. While mechanosensitive Piezo ion channels have been clearly implicated in sensing static touch, their roles in transducing repetitive stimulations are less clear. Here, we perform electrophysiological recordings of heterologously expressed mouse Piezo1 and Piezo2 responding to repetitive mechanical stimulations...
June 20, 2017: Cell Reports
https://www.readbyqxmd.com/read/28636714/characteristics-and-alternative-treatment-outcomes-of-antimalarial-refractory-cutaneous-lupus-erythematosus
#16
Renee Fruchter, Drew J B Kurtzman, Mital Patel, Joseph Merola, Andrew G Franks, Ruth Ann Vleugels, Alisa N Femia
No abstract text is available yet for this article.
June 21, 2017: JAMA Dermatology
https://www.readbyqxmd.com/read/28630947/evolution-of-geographic-atrophy-in-participants-treated-with-ranibizumab-for-neovascular-age-related-macular-degeneration
#17
Alisa T Thavikulwat, Naima Jacobs-El, Jane S Kim, Elvira Agrón, Jesia Hasan, Catherine B Meyerle, David Valent, Catherine A Cukras, Henry E Wiley, Wai T Wong, Emily Y Chew
PURPOSE: To evaluate the risk factors, incidence, and rate of progression of geographic atrophy (GA) in eyes with neovascular age-related macular degeneration (nAMD) treated with ranibizumab. DESIGN: Post-hoc analysis of a prospective clinical study. PARTICIPANTS: 69 participants with nAMD in at least one eye. METHODS: Participants were prospectively treated in the study eye with 0.5 mg intravitreal ranibizumab. Study eyes received 4 monthly injections followed by pro re nata injections until a fluid-free macula was achieved on optical coherence tomography...
January 2017: Ophthalmology retina
https://www.readbyqxmd.com/read/28616700/evaluation-of-irradiation-effects-of-near-infrared-free-electron-laser-of-silver-alloy-for-dental-application
#18
Takao Kuwada-Kusunose, Alisa Kusunose, Masanobu Wakami, Chikako Takebayashi, Haruhiko Goto, Masahiro Aida, Takeshi Sakai, Keisuke Nakao, Kyoko Nogami, Manabu Inagaki, Ken Hayakawa, Kunihiro Suzuki, Toshiro Sakae
In the application of lasers in dentistry, there is a delicate balance between the benefits gained from laser treatment and the heat-related damage arising from laser irradiation. Hence, it is necessary to understand the different processes associated with the irradiation of lasers on dental materials. To obtain insight for the development of a safe and general-purpose laser for dentistry, the present study examines the physical effects associated with the irradiation of a near-infrared free-electron laser (FEL) on the surface of a commonly used silver dental alloy...
June 14, 2017: Lasers in Medical Science
https://www.readbyqxmd.com/read/28615206/plasticity-and-epitope-exposure-of-the-hiv-1-envelope-trimer
#19
Rebecca L R Powell, Maxim Totrov, Vincenza Itri, Xiaomei Liu, Alisa Fox, Susan Zolla-Pazner
We recently showed that mutations in the HIV-1 Envelope (Env) destabilize the V3 loop, rendering neutralization-resistant viruses sensitive to V3-directed monoclonal antibodies (mAbs). Here we investigated the propagation of this effect on other Env epitopes, with special emphasis on V2 loop exposure. Wildtype JR-FL and 19 mutant JR-FL pseudoviruses were tested for neutralization sensitivity to 21 mAbs specific for epitopes in V2, the CD4 binding site (CD4bs), and the CD4-induced (CD4i) region. Certain glycan mutants, mutations in the gp120 hydrophobic core, and mutations in residues involved in intra-protomer interactions exposed epitopes in the V2i region (overlays the α4β7 integrin binding site) and the V3 crown, suggesting a general destabilization of the distal region of the trimer apex...
June 14, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28610121/a-new-species-of-velvet-skate-i-notoraja-i-i-sereti-i-n-sp-rajiformes-arhynchobatidae-from-papua-new-guinea
#20
William T White, Peter R Last, Ralph R Mana
A new arhynchobatin skate, Notoraja sereti n. sp., is described based on three specimens collected from off Madang (Papua New Guinea) at depths of 800-980 m. This medium-size Notoraja skate shares with other velcro skates from the Western Pacific, N. alisae, N. fijiensis, N. inusitata and N. longiventralis, a ventral surface covering of fine denticles giving the skin a velvety feel. Notoraja sereti differs from all of these species in having a shorter snout (preorbital length 10.1-11.1 vs. 11.5-14.5% TL, prenasal length 8...
March 19, 2017: Zootaxa
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