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https://www.readbyqxmd.com/read/28339040/detection-assay-for-hpv16-and-hpv18-by-loop%C3%A2-mediated-isothermal-amplification-with-lateral-flow-dipstick-tests
#1
Ratchanida Kumvongpin, Patcharee Jearanaikoon, Chotchana Wilailuckana, Nattaya Sae-Ung, Prinya Prasongdee, Sakda Daduang, Metee Wongsena, Patcharee Boonsiri, Wansika Kiatpathomchai, Sukumarn Sanersak Swangvaree, Alisa Sandee, Jureerut Daduang
Cervical cancer is the third highest cause of death in developing countries and most commonly results from high‑risk human papillomavirus (HR‑HPV) infection. Among HR‑HPV genotypes, HPV16 and HPV18 are the most prevalent in cervical cancers. Therefore, the present study aimed to develop a detection assay for HPV16 and HPV18 infection using loop‑mediated isothermal amplification (LAMP) with lateral flow dipstick (LFD) tests. This assay is a simplified, user‑friendly method for the visual detection of HPV genotypes...
March 23, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28329550/a-case-of-perniosis
#2
Euphemia W Mu, Vitaly Terushkin, Shane A Meehan, Marie Leger, Alisa Femia
Perniosis is a localized, inflammatory reaction that ischaracterized by erythematous papules and nodulesthat often are located on the acral surfaces in youngwomen. The lesions of perniosis are thought to bedue to cold-induced vasoconstriction that leadsto hypoxemia and inflammation of the vessel wall.Histopathologic and laboratory studies are indicatedfor patients with suspected perniosis to distinguishbetween idiopathic perniosis and secondaryperniosis. Treatment includes adequate heating andclothing, proper food intake, nifedipine, ultravioletA1 phototherapy, topical glucocorticoids, andvasodilators...
December 15, 2016: Dermatology Online Journal
https://www.readbyqxmd.com/read/28329548/sarcoidosis-with-prominent-necrosis-on-histopathology
#3
Rachel Rosenstein, Charisse Orme, Randie H Kim, Shane A Meehan, Alisa Femia
Sarcoidosis is a multiorgan inflammatory diseasewith variable clinical presentations and the commonhistopathologic finding of noncaseating granulomas.The etiology of the disease is not known, butevidence suggests both environmental and geneticcontributions to the pathogenesis. Depending onthe severity of cutaneous disease and extent ofextracutaneous involvement, therapies range fromtopical and intralesional glucocorticoids to systemicimmunomodulatory and immunosuppressiveagents. We present the case of a patient withcutaneous sarcoidosis with prominent necrosis onhistopathologic examination in the setting of severepulmonary sarcoidosis...
December 15, 2016: Dermatology Online Journal
https://www.readbyqxmd.com/read/28329542/silicone-granulomas-with-ulcers
#4
Lisa R Rothman, Randie H Kim, Shane A Meehan, Alisa Femia
We present a 42-year-old transgender womanwith woody induration over her buttocks andlower extremities as well as persistent ulcers of thebuttocks. The lesions developed ten years prior to herpresentation and approximately five years after shereceived illegal silicone injections to her buttocks.Histopathologic examination was consistent witha silicone granuloma. Silicone granuloma is a notan uncommon side effect of silicone injections andmore often occurs when the filler is administeredby non-physician practitioners, as is the case in thispatient...
December 15, 2016: Dermatology Online Journal
https://www.readbyqxmd.com/read/28329540/acral-keratoses-and-squamous-cell-carcinomas-likely-associated-with-arsenic-exposure
#5
Amelia K Hausauer, Rachel Hoffmann, Vitaly Terushkin, Shane A Meehan, Alisa N Femia, Miriam Keltz Pomeranz
Chronic arsenic exposure is known to inducepunctate keratoses with an increased risk ofprimary squamous-cell carcinoma. Drinking wateris currently the major source of arsenic exposureworldwide and is considered one of the mostsubstantial environmental carcinogens. We describethe case of a 61-year-old Hungarian woman withscattered, acral, hyperkeratotic papules and a historyof five palmoplantar squamous-cell carcinomasas well as two other extremity non-melanomaskin cancers. Prior to immigration, she had livedin a county of Southern Hungary that is known tohave elevated concentrations of inorganic arsenicin the drinking water above the World HealthOrganization's current maximum threshold forsafety...
December 15, 2016: Dermatology Online Journal
https://www.readbyqxmd.com/read/28328579/resting-state-functional-connectivity-predicts-longitudinal-pain-symptom-change-in-urologic-chronic-pelvic-pain-syndrome-a-mapp-network-study
#6
Jason J Kutch, Jennifer S Labus, Richard E Harris, Katherine T Martucci, Melissa A Farmer, Sonja Fenske, Connor Fling, Eric Ichesco, Scott Peltier, Bogdan Petre, Wensheng Guo, Xiaoling Hou, Alisa J Stephens, Chris Mullins, Daniel J Clauw, Sean C Mackey, A Vania Apkarian, J Richard Landis, Emeran A Mayer
Chronic pain symptoms often change over time, even in individuals who have had symptoms for years. Studying biological factors that predict trends in symptom change in chronic pain may uncover novel pathophysiological mechanisms and potential therapeutic targets. In this study, we investigated whether brain functional connectivity measures obtained from resting-state functional MRI (rs-fMRI) at baseline can predict longitudinal symptom change (3, 6, and 12 months post-scan) in urologic chronic pelvic pain syndrome (UCPPS)...
March 17, 2017: Pain
https://www.readbyqxmd.com/read/28319273/gastric-microbiota-features-associated-with-cancer-risk-factors-and-clinical-outcomes-a-pilot-study-in-gastric-cardia-cancer-patients-from-shanxi-china
#7
Guoqin Yu, Nan Hu, Lemin Wang, Chaoyu Wang, Xiao-You Han, Mike Humphry, Jacques Ravel, Christian C Abnet, Philip R Taylor, Alisa M Goldstein
Little is known about the link between gastric microbiota and the epidemiology of gastric cancer. In order to determine the epidemiologic and clinical relevance of gastric microbiota, we used 16S ribosomal RNA gene sequencing analysis to characterize the composition and structure of the gastric microbial community of 80 paired samples (non-malignant and matched tumor tissues) from gastric cardia adenocarcinoma (GCA) patients in Shanxi, China. We also used PICRUSt to predict microbial functional profiles. Compared to patients without family history of upper gastrointestinal (UGI) cancer in the non-malignant gastric tissue microbiota, patients with family history of UGI cancer had higher Helicobacter pylori (Hp) relative abundance (median: 0...
March 20, 2017: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/28317076/an-experimental-tool-to-estimate-the-probability-of-a-nucleotide-presence-in-the-crystal-structures-of-the-nucleotide-protein-complexes
#8
Maria Nemchinova, Vitaly Balobanov, Ekaterina Nikonova, Natalia Lekontseva, Alisa Mikhaylina, Svetlana Tishchenko, Alexey Nikulin
A correlation between the ligand-protein affinity and the identification of the ligand in the experimental electron density maps obtained by X-ray crystallography has been tested for a number of RNA-binding proteins. Bacterial translation regulators ProQ, TRAP, Rop, and Hfq together with their archaeal homologues SmAP have been used. The equilibrium dissociation constants for the N-methyl-anthraniloyl-labelled adenosine and guanosine monophosphates titrated by the proteins have been determined by the fluorescent anisotropy measurements...
March 20, 2017: Protein Journal
https://www.readbyqxmd.com/read/28314784/integrative-cancer-pharmacogenomics-to-infer-large-scale-drug-taxonomy
#9
Nehme El-Hachem, Deena M A Gendoo, Laleh Soltan Ghoraie, Zhaleh Safikhani, Petr Smirnov, Christina Chung, Kenan Deng, Alisa Fang, Erin Birkwood, Chantal Ho, Ruth Isserlin, Gary Bader, Anna Goldenberg, Benjamin Haibe-Kains
Identification of drug targets and mechanism of action (MoA) for new and uncharacterized anticancer drugs is important for optimization of treatment efficacy. Current MoA prediction largely relies on prior information including side effects, therapeutic indication, and chemo-informatics. Such information is not transferable or applicable for newly identified, previously uncharacterized small molecules. Therefore, a shift in the paradigm of MoA predictions is necessary towards development of unbiased approaches that can elucidate drug relationships and efficiently classify new compounds with basic input data...
March 17, 2017: Cancer Research
https://www.readbyqxmd.com/read/28277348/groove-sign-of-eosinophilic-fasciitis
#10
Renee Fruchter, Daniel R Mazori, Alisa N Femia
No abstract text is available yet for this article.
April 2017: Journal of Clinical Rheumatology: Practical Reports on Rheumatic & Musculoskeletal Diseases
https://www.readbyqxmd.com/read/28270455/testifying-about-trauma-a-call-for-science-and-civility
#11
EDITORIAL
Kenneth J Weiss, Alisa R Gutman
No abstract text is available yet for this article.
March 2017: Journal of the American Academy of Psychiatry and the Law
https://www.readbyqxmd.com/read/28268250/antioxidant-effect-of-thiazolidine-molecules-in-cell-culture-media-improves-stability-and-performance
#12
Jennifer Kuschelewski, Alisa Schnellbaecher, Sascha Pering, Maria Wehsling, Aline Zimmer
The ability of cell culture media components to generate reactive species as well as their sensitivity to oxidative degradation, affects the overall stability of media and the behavior of cells cultured in vitro. This study investigates the influence of thiazolidine molecules, formed from the condensation between cysteine and alpha-ketoacids, on the stability of these complex mixtures and on the performance of cell culture processes aiming to produce therapeutically relevant monoclonal antibodies. Results presented in this study indicate that 2-methyl-1,3-thiazolidine-2,4-dicarboxylic acid and 2-(2-carboxyethyl)-1,3-thiazolidine-2,4-dicarboxylic acid, obtained by condensation of cysteine with pyruvate or alpha-ketoglutarate respectively, are able to stabilize cell culture media formulations, in particular redox sensitive molecules like folic acid, thiamine, L-methionine (met) and L-tryptophan (trp)...
March 7, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28251913/elevated-hematocrit-enhances-platelet-accumulation-following-vascular-injury
#13
Bethany L Walton, Marcus Lehmann, Tyler Skorczewski, Lori A Holle, Joan D Beckman, Jeremy A Cribb, Micah J Mooberry, Adam R Wufsus, Brian C Cooley, Jonathan W Homeister, Rafal Pawlinski, Michael R Falvo, Nigel S Key, Aaron L Fogelson, Keith B Neeves, Alisa S Wolberg
Red blood cells (RBCs) demonstrate procoagulant properties in vitro, and elevated hematocrit is associated with reduced bleeding and increased thrombosis risk in humans. These observations suggest RBCs contribute to thrombus formation. However, effects of RBCs on thrombosis are difficult to assess because humans and mice with elevated hematocrit typically have co-existing pathologies. Using an experimental model of elevated hematocrit in healthy mice, we measured effects of hematocrit in two in vivo clot formation models...
March 1, 2017: Blood
https://www.readbyqxmd.com/read/28248897/measurement-properties-of-the-nih-minimal-dataset-dutch-language-version-in-patients-with-chronic-low-back-pain
#14
Annemarie Boer, Alisa L Dutmer, Henrica R Schiphorst Preuper, Lucas H V van der Woude, Roy E Stewart, Richard A Deyo, Michiel F Reneman, Remko Soer
STUDY DESIGN: Validation study with cross sectional and longitudinal measurements OBJECTIVE.: To translate the US National Institute of Health (NIH)-minimal dataset for clinical research on chronic low back pain into the Dutch language and to test its validity and reliability among people with chronic low back pain. SUMMARY OF BACKGROUND DATA: The NIH developed a minimal dataset to encourage more complete and consistent reporting of clinical research and to be able to compare studies across countries in patients with low back pain...
February 28, 2017: Spine
https://www.readbyqxmd.com/read/28241356/testosterone-treatment-and-cognitive-function-in-older-men-with-low-testosterone-and-age-associated-memory-impairment
#15
RANDOMIZED CONTROLLED TRIAL
Susan M Resnick, Alvin M Matsumoto, Alisa J Stephens-Shields, Susan S Ellenberg, Thomas M Gill, Sally A Shumaker, Debbie D Pleasants, Elizabeth Barrett-Connor, Shalender Bhasin, Jane A Cauley, David Cella, Jill P Crandall, Glenn R Cunningham, Kristine E Ensrud, John T Farrar, Cora E Lewis, Mark E Molitch, Marco Pahor, Ronald S Swerdloff, Denise Cifelli, Stephen Anton, Shehzad Basaria, Susan J Diem, Christina Wang, Xiaoling Hou, Peter J Snyder
Importance: Most cognitive functions decline with age. Prior studies suggest that testosterone treatment may improve these functions. Objective: To determine if testosterone treatment compared with placebo is associated with improved verbal memory and other cognitive functions in older men with low testosterone and age-associated memory impairment (AAMI). Design, Setting, and Participants: The Testosterone Trials (TTrials) were 7 trials to assess the efficacy of testosterone treatment in older men with low testosterone levels...
February 21, 2017: JAMA: the Journal of the American Medical Association
https://www.readbyqxmd.com/read/28241355/testosterone-treatment-and-coronary-artery-plaque-volume-in-older-men-with-low-testosterone
#16
RANDOMIZED CONTROLLED TRIAL
Matthew J Budoff, Susan S Ellenberg, Cora E Lewis, Emile R Mohler, Nanette K Wenger, Shalender Bhasin, Elizabeth Barrett-Connor, Ronald S Swerdloff, Alisa Stephens-Shields, Jane A Cauley, Jill P Crandall, Glenn R Cunningham, Kristine E Ensrud, Thomas M Gill, Alvin M Matsumoto, Mark E Molitch, Rine Nakanishi, Negin Nezarat, Suguru Matsumoto, Xiaoling Hou, Shehzad Basaria, Susan J Diem, Christina Wang, Denise Cifelli, Peter J Snyder
Importance: Recent studies have yielded conflicting results as to whether testosterone treatment increases cardiovascular risk. Objective: To test the hypothesis that testosterone treatment of older men with low testosterone slows progression of noncalcified coronary artery plaque volume. Design, Setting, and Participants: Double-blinded, placebo-controlled trial at 9 academic medical centers in the United States. The participants were 170 of 788 men aged 65 years or older with an average of 2 serum testosterone levels lower than 275 ng/dL (82 men assigned to placebo, 88 to testosterone) and symptoms suggestive of hypogonadism who were enrolled in the Testosterone Trials between June 24, 2010, and June 9, 2014...
February 21, 2017: JAMA: the Journal of the American Medical Association
https://www.readbyqxmd.com/read/28241237/association-of-testosterone-levels-with-anemia-in-older-men-a-controlled-clinical-trial
#17
Cindy N Roy, Peter J Snyder, Alisa J Stephens-Shields, Andrew S Artz, Shalender Bhasin, Harvey J Cohen, John T Farrar, Thomas M Gill, Bret Zeldow, David Cella, Elizabeth Barrett-Connor, Jane A Cauley, Jill P Crandall, Glenn R Cunningham, Kristine E Ensrud, Cora E Lewis, Alvin M Matsumoto, Mark E Molitch, Marco Pahor, Ronald S Swerdloff, Denise Cifelli, Xiaoling Hou, Susan M Resnick, Jeremy D Walston, Stephen Anton, Shehzad Basaria, Susan J Diem, Christina Wang, Stanley L Schrier, Susan S Ellenberg
Importance: In one-third of older men with anemia, no recognized cause can be found. Objective: To determine if testosterone treatment of men 65 years or older with unequivocally low testosterone levels and unexplained anemia would increase their hemoglobin concentration. Design, Setting, and Participants: A double-blinded, placebo-controlled trial with treatment allocation by minimization using 788 men 65 years or older who have average testosterone levels of less than 275 ng/dL...
February 21, 2017: JAMA Internal Medicine
https://www.readbyqxmd.com/read/28241231/effect-of-testosterone-treatment-on-volumetric-bone-density-and-strength-in-older-men-with-low-testosterone-a-controlled-clinical-trial
#18
Peter J Snyder, David L Kopperdahl, Alisa J Stephens-Shields, Susan S Ellenberg, Jane A Cauley, Kristine E Ensrud, Cora E Lewis, Elizabeth Barrett-Connor, Ann V Schwartz, David C Lee, Shalender Bhasin, Glenn R Cunningham, Thomas M Gill, Alvin M Matsumoto, Ronald S Swerdloff, Shehzad Basaria, Susan J Diem, Christina Wang, Xiaoling Hou, Denise Cifelli, Darlene Dougar, Bret Zeldow, Douglas C Bauer, Tony M Keaveny
Importance: As men age, they experience decreased serum testosterone concentrations, decreased bone mineral density (BMD), and increased risk of fracture. Objective: To determine whether testosterone treatment of older men with low testosterone increases volumetric BMD (vBMD) and estimated bone strength. Design, Setting, and Participants: Placebo-controlled, double-blind trial with treatment allocation by minimization at 9 US academic medical centers of men 65 years or older with 2 testosterone concentrations averaging less than 275 ng/L participating in the Testosterone Trials from December 2011 to June 2014...
February 21, 2017: JAMA Internal Medicine
https://www.readbyqxmd.com/read/28241211/families-as-partners-in-hospital-error-and-adverse-event-surveillance
#19
Alisa Khan, Maitreya Coffey, Katherine P Litterer, Jennifer D Baird, Stephannie L Furtak, Briana M Garcia, Michele A Ashland, Sharon Calaman, Nicholas C Kuzma, Jennifer K O'Toole, Aarti Patel, Glenn Rosenbluth, Lauren A Destino, Jennifer L Everhart, Brian P Good, Jennifer H Hepps, Anuj K Dalal, Stuart R Lipsitz, Catherine S Yoon, Katherine R Zigmont, Rajendu Srivastava, Amy J Starmer, Theodore C Sectish, Nancy D Spector, Daniel C West, Christopher P Landrigan, Brenda K Allair, Claire Alminde, Wilma Alvarado-Little, Marisa Atsatt, Megan E Aylor, James F Bale, Dorene Balmer, Kevin T Barton, Carolyn Beck, Zia Bismilla, Rebecca L Blankenberg, Debra Chandler, Amanda Choudhary, Eileen Christensen, Sally Coghlan-McDonald, F Sessions Cole, Elizabeth Corless, Sharon Cray, Roxi Da Silva, Devesh Dahale, Benard Dreyer, Amanda S Growdon, LeAnn Gubler, Amy Guiot, Roben Harris, Helen Haskell, Irene Kocolas, Elizabeth Kruvand, Michele Marie Lane, Kathleen Langrish, Christy J W Ledford, Kheyandra Lewis, Joseph O Lopreiato, Christopher G Maloney, Amanda Mangan, Peggy Markle, Fernando Mendoza, Dale Ann Micalizzi, Vineeta Mittal, Maria Obermeyer, Katherine A O'Donnell, Mary Ottolini, Shilpa J Patel, Rita Pickler, Jayne Elizabeth Rogers, Lee M Sanders, Kimberly Sauder, Samir S Shah, Meesha Sharma, Arabella Simpkin, Anupama Subramony, E Douglas Thompson, Laura Trueman, Tanner Trujillo, Michael P Turmelle, Cindy Warnick, Chelsea Welch, Andrew J White, Matthew F Wien, Ariel S Winn, Stephanie Wintch, Michael Wolf, H Shonna Yin, Clifton E Yu
Importance: Medical errors and adverse events (AEs) are common among hospitalized children. While clinician reports are the foundation of operational hospital safety surveillance and a key component of multifaceted research surveillance, patient and family reports are not routinely gathered. We hypothesized that a novel family-reporting mechanism would improve incident detection. Objective: To compare error and AE rates (1) gathered systematically with vs without family reporting, (2) reported by families vs clinicians, and (3) reported by families vs hospital incident reports...
February 27, 2017: JAMA Pediatrics
https://www.readbyqxmd.com/read/28228446/fibrinogen-and-fibrin-in-hemostasis-and-thrombosis
#20
Sravya Kattula, James R Byrnes, Alisa S Wolberg
No abstract text is available yet for this article.
March 2017: Arteriosclerosis, Thrombosis, and Vascular Biology
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