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https://www.readbyqxmd.com/read/28075467/resveratrol-suppresses-human-hepatocellular-carcinoma-via-targeting-hgf-c-met-signaling-pathway
#1
Feng Gao, Gang Deng, Wenbin Liu, Kechao Zhou, Ming Li
Resveratrol, one of the major polyphenols found in red wine, is suggested to have a role as a chemo-prevention or chemotherapy agent in various human cancer models. Herein, we report that resveratrol has a profound antitumor effect on human hepatocellular carcinoma (HCC) cells by down-regulation of the HGF-c-Met signaling pathway. Resveratrol inhibited anchorage-dependent and -independent growth of HCC cells in a dose-dependent manner. Short-term resveratrol exposure substantially decreased HGF-induced c-Met signaling pathway activation, and long-term exposure to resveratrol markedly inhibited c-Met expression on the cell membrane...
February 2017: Oncology Reports
https://www.readbyqxmd.com/read/28002613/different-responses-of-vanillic-acid-a-phenolic-compound-in-htc-cells-cytotoxicity-antiproliferative-activity-and-protection-from-dna-induced-damage
#2
I V Almeida, F M L Cavalcante, V E P Vicentini
The consumption of healthy and natural foods has increased over the last few years, primarily because these foods are rich in substances with biological properties of interest, such as exerting anticancer effects and decreasing oxidative stress in living tissues. These foods support adequate nutrition, maintain health, and improve quality of life. Vanillic acid (VA) is a phenolic compound used widely in the food industry as a flavoring, preservative, and food additive. VA can be found in various cereals, whole grains, fruits, herbs, green tea, juices, beers, and wines and possesses antioxidant, hepatoprotective, cardioprotective, and antiapoptotic activities...
December 19, 2016: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/27992098/candida-pyralidae-killer-toxin-disrupts-the-cell-wall-of-brettanomyces-bruxellensis-in-red-grape-juice
#3
N N Mehlomakulu, K J Prior, M E Setati, B Divol
AIMS: The control of the wine spoilage yeast Brettanomyces bruxellensis using biological methods such as killer toxins (instead of the traditional chemical methods e.g. SO2 ) has been the focus of several studies within the last decade. Our previous research demonstrated that the killer toxins CpKT1 and CpKT2 isolated from the wine yeast Candida pyralidae were active and stable under winemaking conditions. In the current study, we report the possible mode of action of CpKT1 on B. bruxellensis cells in red grape juice...
December 19, 2016: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/27979050/comparison-of-the-physicochemical-and-volatile-composition-of-wine-fractions-obtained-by-two-different-dealcoholization-techniques
#4
Silvia Motta, Massimo Guaita, Maurizio Petrozziello, Aldo Ciambotti, Loretta Panero, Mauro Solomita, Antonella Bosso
Three wines (a Rosé wine, a Pelaverga and a Barbera red wines) were dealcoholized at 5% v/v ethanol with two different techniques: membrane contactor (MC) and distillation under vacuum (D). The influence of the treatments on the physicochemical composition and aromatic profile of the dealcoholized fractions was investigated. The main difference between the two techniques was the concentration grade reached by the dealcoholized fractions, which was 5-6times higher with D than with MC due to the concomitant loss of water...
April 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/27933053/waste-conversion-into-n-caprylate-and-n-caproate-resource-recovery-from-wine-lees-using-anaerobic-reactor-microbiomes-and-in-line-extraction
#5
Leo A Kucek, Jiajie Xu, Mytien Nguyen, Largus T Angenent
To convert wastes into sustainable liquid fuels and chemicals, new resource recovery technologies are required. Chain elongation is a carboxylate-platform bioprocess that converts short-chain carboxylates (SCCs) (e.g., acetate [C2] and n-butyrate [C4]) into medium-chain carboxylates (MCCs) (e.g., n-caprylate [C8] and n-caproate [C6]) with hydrogen gas as a side product. Ethanol or another electron donor (e.g., lactate, carbohydrate) is required. Competitive MCC productivities, yields (product vs. substrate fed), and specificities (product vs...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27763562/adsorption-of-wine-constituents-on-functionalized-surfaces
#6
Agnieszka Mierczynska-Vasilev, Paul A Smith
The adsorption of macromolecules on solid surfaces is of great importance in the field of nanotechnology, biomaterials, biotechnological, and food processes. In the field of oenology adsorption of wine macromolecules such as polyphenols, polysaccharides, and proteins is much less desirable on membrane materials because of fouling and reduced filtering performance. On the other hand, adsorption of these molecules on processing aids is very beneficial for achieving wine clarity and stability. In this article, the effect of surface chemical functionalities on the adsorption of white, rosé, and red wine constituents was evaluated...
October 18, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27704093/smart-sensory-materials-for-divalent-cations-a-dithizone-immobilized-membrane-for-optical-analysis
#7
Giancarla Alberti, Silvia Re, Anna Maria Chiara Tivelli, Raffaela Biesuz
An optode for Cu(ii), Cd(ii), Zn(ii) and Hg(ii) sensing has been designed by immobilization of dithizone on a triacetylcellulose membrane (mem-DTZ). The sorption of the metal ions on the mem-DTZ has been thoroughly characterized, in particular sorption kinetics, sorption isotherms and profiles as a function of the pH have been studied. Methods to assess the concentrations of the cations, both individually and in a mixture, in unknown samples, have been developed. In particular, UV-vis spectra and digital information of pictures taken by using a common desktop scanner, of the mem-DTZ sensor after equilibration with different solutions, were acquired and they were correlated with the concentration of the metal ions in solutions...
October 17, 2016: Analyst
https://www.readbyqxmd.com/read/27598001/comparative-morphological-characteristics-of-three-brettanomyces-bruxellensis-wine-strains-in-the-presence-absence-of-sulfur-dioxide
#8
Marli Louw, Maret du Toit, Hervé Alexandre, Benoit Divol
The red wine spoilage yeast Brettanomyces bruxellensis has been the subject of numerous investigations. Some of these studies focused on spoilage mechanisms, sulfur dioxide tolerance and nutrient requirements. Pseudomycelium formation, although a striking feature of this species, has however been poorly investigated. Furthermore, literature regarding the induction mechanism of pseudomycelium formation in this yeast is limited and lacks clarity, as results published are contradictory. This study elucidates this phenomenon among strains from geographically different areas...
December 5, 2016: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/27534113/red-wine-inhibits-aggregation-and-increases-atp-diphosphohydrolase-cd39-activity-of-rat-platelets-in-vitro
#9
Elisabetta Caiazzo, Idolo Tedesco, Carmela Spagnuolo, Gian Luigi Russo, Armando Ialenti, Carla Cicala
Moderate consumption of red wine has been shown to exert a peculiar cardioprotective effect compared with other alcoholic beverages; inhibition of platelet aggregation seems to be one of the mechanisms underlying this beneficial effect. CD39/ATP-diphosphohydrolase is an integral membrane glycoprotein metabolizing ATP and ADP to AMP; in concert with CD73/ecto-5'-nucleotidase, it contributes to extracellular adenosine accumulation. CD39 is considered a key modulator of thrombus formation; it inhibits platelet aggregation by promoting ADP hydrolysis...
June 2016: Natural Product Communications
https://www.readbyqxmd.com/read/27502415/antimicrobial-properties-and-death-inducing-mechanisms-of-saccharomycin-a-biocide-secreted-by-saccharomyces-cerevisiae
#10
Patrícia Branco, Diana Francisco, Margarida Monteiro, Maria Gabriela Almeida, Jorge Caldeira, Nils Arneborg, Catarina Prista, Helena Albergaria
We recently found that Saccharomyces cerevisiae (strain CCMI 885) secretes antimicrobial peptides (AMPs) derived from the glycolytic enzyme glyceraldehyde 3-phosphate dehydrogenase (GAPDH) that are active against various wine-related yeast and bacteria. Here, we show that several other S. cerevisiae strains also secrete natural biocide fractions during alcoholic fermentation, although at different levels, which correlates with the antagonistic effect exerted against non-Saccharomyces yeasts. We, therefore, term this biocide saccharomycin...
January 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27406567/lps-remodeling-triggers-formation-of-outer-membrane-vesicles-in-salmonella
#11
Wael Elhenawy, Michael Bording-Jorgensen, Ezequiel Valguarnera, M Florencia Haurat, Eytan Wine, Mario F Feldman
UNLABELLED: Outer membrane vesicles (OMV) are proposed to mediate multiple functions during pathogenesis and symbiosis. However, the mechanisms responsible for OMV formation remain poorly understood. It has been shown in eukaryotic membranes that lipids with an inverted-cone shape favor the formation of positive membrane curvatures. Based on these studies, we formulated the hypothesis that lipid A deacylation might impose shape modifications that result in the curvature of the outer membrane (OM) and subsequent OMV formation...
2016: MBio
https://www.readbyqxmd.com/read/27402289/grape-tannin-catechin-and-ethanol-fluidify-oral-membrane-mimics-containing-moderate-amounts-of-cholesterol-implications-on-wine-tasting
#12
Aurélien L Furlan, Ahmad Saad, Erick J Dufourc, Julie Géan
Wine tasting results in interactions of tannin-ethanol solutions with proteins and lipids of the oral cavity. Among the various feelings perceived during tasting, astringency and bitterness most probably result in binding events with saliva proteins, lipids and receptors. In this work, we monitored the conjugated effect of the grape polyphenol catechin and ethanol on lipid membranes mimicking the different degrees of keratinization of oral cavity surfaces by varying the amount of cholesterol present in membranes...
November 2016: Biochimie
https://www.readbyqxmd.com/read/27343571/automatic-bionalyzer-using-an-integrated-amperometric-biosensor-for-the-determination-of-l-malic-acid-in-wines
#13
E Vargas, M A Ruiz, F J Ferrero, S Campuzano, V Ruiz-Valdepeñas Montiel, A J Reviejo, J M Pingarrón
A new automatic bioanalyzer for L-malic acid using an integrated amperometric biosensor as detector is reported for the first time in this work. The biosensor is constructed by gold film sputtering deposition on a stainless steel disk electrode and co-immobilization of the enzymes malate dehydrogenase (MDH) and diaphorase (DP) together with the redox mediator tetrathiafulvalene (TTF) by means of dialysis membrane. The analytical performance of the biosensor was evaluated when it was used as amperometric detector in three different analytical methodologies: stirred solutions, semiautomatic FIA system and automatic bioanalyzer...
September 1, 2016: Talanta
https://www.readbyqxmd.com/read/27314384/piceatannol-and-other-wine-stilbenes-a-pool-of-inhibitors-against-%C3%AE-synuclein-aggregation-and-cytotoxicity
#14
Hamza Temsamani, Stéphanie Krisa, Marion Decossas-Mendoza, Olivier Lambert, Jean-Michel Mérillon, Tristan Richard
The aggregation of α-synuclein is one on the key pathogenic events in Parkinson's disease. In the present study, we investigated the inhibitory capacities of stilbenes against α-synuclein aggregation and toxicity. Thioflavin T fluorescence, transmission electronic microscopy, and SDS-PAGE analysis were performed to investigate the inhibitory effects of three stilbenes against α-synuclein aggregation: piceatannol, ampelopsin A, and isohopeaphenol. Lipid vesicle permeabilization assays were performed to screen stilbenes for protection against membrane damage induced by aggregated α-synuclein...
June 15, 2016: Nutrients
https://www.readbyqxmd.com/read/27282752/low-cost-and-compact-analytical-microsystem-for-carbon-dioxide-determination-in-production-processes-of-wine-and-beer
#15
Antonio Calvo-López, Oriol Ymbern, David Izquierdo, Julián Alonso-Chamarro
The design, construction and evaluation of a low cost, cyclic olefin copolymer (COC)-based continuous flow microanalyzer, with optical detection, to monitor carbon dioxide in bottled wines and beers as well as in fermentation processes, is presented. The microsystem, constructed by computer numerically controlled (CNC) micromilling and using a multilayer approach, integrates microfluidics, gas-diffusion module and an optical flow-cell in a single polymeric substrate. Its size is slightly bigger than a credit card, exactly 45 × 60 × 4 mm in the microfluidic and diffusion module zone and 22...
August 10, 2016: Analytica Chimica Acta
https://www.readbyqxmd.com/read/27262281/removal-of-pesticides-from-white-and-red-wines-by-microfiltration
#16
Danae S Doulia, Efstathios K Anagnos, Konstantinos S Liapis, Demetrios A Klimentzos
The aim of this work is the investigation of microfiltration in removing pesticides from a white and a red Greek wine. Six membranes with pore size 0.45μm were investigated. Two mixtures of 23 and 9 pesticides, and single pesticide solutions were added in the wine. The pesticides tested belong to 11 chemical groups. Solid phase extraction (SPE) followed by gas chromatography (GC) with electron capture detector (ECD) were performed to analyze pesticide residues of the filtered fortified wine. Distinct behavior was exhibited by each membrane...
November 5, 2016: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/27112541/influence-of-the-presence-of-ethanol-on-in%C3%A2-vitro-bioavailability-of-fungicide-residues
#17
S Cermeño, G Martínez, J Oliva, M A Cámara, A Barba
The influence of ethanol on the in vitro bioavailability of nine fungicides in wines of varying degrees of alcohol is studied by simulating the digestive process by dialysis in semipermeable membranes. The dose of each fungicide corresponds to its added Maximum Residue Limits in the different matrices (water, ethanol, wines of 7, 11, 13 and 14.5% ethanol). A validated analytical methodology was used which includes extraction, partition according to the modified QuEChERS multiresidue method and liquid chromatography with triple quadrupole tandem mass spectrometry analysis...
July 2016: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/27098726/changes-in-volatile-composition-and-sensory-attributes-of-wines-during-alcohol-content-reduction
#18
Rocco Longo, John W Blackman, Peter J Torley, Suzy Y Rogiers, Leigh M Schmidtke
A desirable sensory profile is a major consumer driver for wine acceptability and should be considered during the production of reduced alcohol wines. Although various viticultural practices and microbiological approaches show promising results, separation technologies such as membrane filtration, in particular reverse osmosis, evaporative perstraction, and vacuum distillation represent the most common commercial methods used to produce reduced alcohol wine. However, ethanol removal from wine can result in a significant loss of volatile compounds such as esters (ethyl octanoate, ethyl acetate, isoamyl acetate) that contribute positively to the overall perceived aroma...
April 21, 2016: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/27056596/partial-dealcoholization-of-red-wine-by-nanofiltration-and-its-effect-on-anthocyanin-and-resveratrol-levels
#19
Szilvia Banvolgyi, K Savaş Bahçeci, Gyula Vatai, Sandor Bekassy, Erika Bekassy-Molnar
The present work studies the use of nanofiltration for the production of red wine concentrate with low alcohol content. Factorial design was applied to measure the influences of transmembrane pressure (10-20 bar) and temperature (20-40 ℃) on the retention of valuable components such as anthocyanins and resveratrol, and on the nanofiltration membrane performance. The highest retention of anthocyanin and resveratrol was achieved at low temperature (20 ℃), while the high transmembrane pressure (20 bar) was found to increase the permeate flux considerably...
December 2016: Food Science and Technology International, Ciencia y Tecnología de Los Alimentos Internacional
https://www.readbyqxmd.com/read/27012485/wine-glasses-and-hourglasses-non-adaptive-complexity-of-vesicle-traffic-in-microbial-eukaryotes
#20
Somya Mani, Mukund Thattai
Microbial eukaryotes present a stunning diversity of endomembrane organization. From specialized secretory organelles such as the rhoptries and micronemes of apicomplexans, to peroxisome-derived metabolic compartments such as the glycosomes of kinetoplastids, different microbial taxa have explored different solutions to the compartmentalization and processing of cargo. The basic secretory and endocytic system, comprising the ER, Golgi, endosomes, and plasma membrane, as well as diverse taxon-specific specialized endomembrane organelles, are coupled by a complex network of cargo transport via vesicle traffic...
September 2016: Molecular and Biochemical Parasitology
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