Chris E Cooper, Gary G A Silkstone, Michelle Simons, Badri Rajagopal, Natalie Syrett, Thoufieq Shaik, Svetlana Gretton, Elizabeth Welbourn, Leif Bülow, Nélida Leiva Eriksson, Luca Ronda, Andrea Mozzarelli, Andras Eke, Domokos Mathe, Brandon J Reeder
Hemoglobin (Hb)-based oxygen carriers (HBOC) are modified extracellular proteins, designed to replace or augment the oxygen-carrying capacity of erythrocytes. However, clinical results have generally been disappointing due to adverse side effects, in part linked to the intrinsic oxidative toxicity of Hb. Previously a redox-active tyrosine residue was engineered into the Hb β subunit (βF41Y) to facilitate electron transfer between endogenous antioxidants such as ascorbate and the oxidative ferryl heme species, converting the highly oxidizing ferryl species into the less reactive ferric (met) form...
December 27, 2018: Free Radical Biology & Medicine