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Cell-free hemoglobin

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https://www.readbyqxmd.com/read/28544589/methionine-tumor-starvation-by-erythrocyte-encapsulated-methionine-gamma-lyase-activity-controlled-with-per-os-vitamin-b6
#1
Fabien Gay, Karine Aguera, Karine Sénéchal, Angie Tainturier, Willy Berlier, Delphine Maucort-Boulch, Jérôme Honnorat, Françoise Horand, Yann Godfrin, Vanessa Bourgeaux
Erymet is a new therapy resulting from the encapsulation of a methionine gamma-lyase (MGL; EC number 4.4.1.11) in red blood cells (RBC). The aim of this study was to evaluate erymet potential efficacy in methionine (Met)-dependent cancers. We produced a highly purified MGL using a cGMP process, determined the pharmacokinetics/pharmacodynamics (PK/PD) properties of erymet in mice, and assessed its efficacy on tumor growth prevention. Cytotoxicity of purified MGL was tested in six cancer cell lines. CD1 mice were injected with single erymet product supplemented or not with vitamin B6 vitamer pyridoxine (PN; a precursor of PLP cofactor)...
May 23, 2017: Cancer Medicine
https://www.readbyqxmd.com/read/28544427/advanced-consumable-free-morphological-analysis-of-intact-red-blood-cells-by-a-compact-scanning-flow-cytometer
#2
K V Gilev, E S Yastrebova, D I Strokotov, M A Yurkin, N A Karmadonova, A V Chernyshev, V V Lomivorotov, V P Maltsev
Whereas modern automated blood cell analyzers measure the volume of individual red blood cells (RBCs), leading to four RBC indices (mean corpuscular volume, MCV; mean corpuscular hemoglobin, MCH; mean corpuscular hemoglobin concentration, MCHC; red cell distribution width, and RDW), the RBC shape has not been assessed by clinical screening tools. We applied the scanning flow cytometer (SFC) for complete characterization of intact RBC morphology in terms of diameter, maximal and minimal thicknesses, volume, surface area, sphericity index, spontaneous curvature, hemoglobin concentration, and content...
May 19, 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/28543744/repetitive-supra-physiological-shear-stress-impairs-red-blood-cell-deformability-and-induces-hemolysis
#3
Jarod T Horobin, Surendran Sabapathy, Michael J Simmonds
The supra-physiological shear stress that blood is exposed to while traversing mechanical circulatory assist devices affects the physical properties of red blood cells (RBCs), impairs RBC deformability, and may induce hemolysis. Previous studies exploring RBC damage following exposure to supra-physiological shear stress have employed durations exceeding clinical instrumentation, thus we explored changes in RBC deformability following exposure to shear stress below the reported "hemolytic threshold" using shear exposure durations per minute (i...
May 23, 2017: Artificial Organs
https://www.readbyqxmd.com/read/28521553/glutaraldehyde-polymerized-hemoglobin-and-tempol-polyhb-tempol-has-superoxide-dismutase-activity-that-can-attenuate-oxidative-stress-on-endothelial-cells-induced-by-superoxide-anion
#4
Mengdi Wu, Kun Feng, Qiuhui Li, Huiya Ma, Hongli Zhu, Yudou Xie, Gaofei Yan, Chao Chen, Kunping Yan
A Tempol compound with an amine group (4-amino-2,2,6,6-tetramethylpiperidine-1-oxyl, NH2-Tempol) was cross-linked to hemoglobin in a one-step polymerization reaction to produce a novel hemoglobin-based oxygen carrier (HBOC) designated PolyHb-Tempol. The reaction parameters, including the reaction time, pH, temperature, and ratio of reactants, were optimized, and the physiochemical properties of the resulting product were characterized. PolyHb-Tempol didn't show any toxicity towards endothelial cells. Furthermore, from observations of cell morphology and viability, PolyHb-Tempol showed a significant ability to inhibit or eliminate oxidative stress induced by superoxide free radicals...
May 18, 2017: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/28482712/heme-mediated-cell-activation-the-inflammatory-puzzle-of-sickle-cell-anemia
#5
Caroline Conceição da Guarda, Rayra Pereira Santiago, Luciana Magalhães Fiuza, Milena Magalhães Aleluia, Júnia Raquel Dutra Ferreira, Camylla Vilas Boas Figueiredo, Setondji Cocou Modeste Alexandre Yahouedehou, Rodrigo Mota de Oliveira, Isa Menezes Lyra, Marilda de Souza Gonçalves
Hemolysis triggers the onset of several clinical manifestations of sickle cell anemia (SCA). During hemolysis, heme, which is derived from hemoglobin (Hb), accumulates due to the inability of detoxification systems to scavenge sufficiently. Heme exerts multiple harmful effects, including leukocyte activation and migration, enhanced adhesion molecule expression by endothelial cells and the production of pro-oxidant molecules. Area covered: In this review, we describe the effects of heme on leukocytes and endothelial cells, as well as the features of vascular endothelial cells related to vaso-occlusion in SCA...
May 17, 2017: Expert Review of Hematology
https://www.readbyqxmd.com/read/28470926/prevalence-of-hemolysis-and-metabolic-acidosis-in-patients-with-circulatory-failure-supported-with-extracorporeal-life-support-a-marker-for-survival
#6
Karla Lehle, Matthias Lubnow, Alois Philipp, Maik Foltan, Florian Zeman, York Zausig, Dirk Lunz, Christof Schmid, Thomas Müller
AIMS: Elevated levels of plasma free hemoglobin (fHb) indicate red blood cell (RBC) damage. The aim of this study was to analyze the prevalence of hemolysis and metabolic acidosis in patients on extracorporeal life support (ECLS) and to investigate whether it is a marker for outcome. METHODS AND RESULTS: This retrospective analysis included 215 adult patients with cardiac failure treated with ECLS. The cohort was divided into three groups: ECLS (1) during ongoing cardiopulmonary resuscitation (CPR, n = 110); (2) after CPR with return of spontaneous circulation and sustained cardiogenic shock (n = 45); (3) in severe cardiogenic shock without previous CPR (n = 60)...
May 2017: European Journal of Heart Failure
https://www.readbyqxmd.com/read/28458720/role-of-red-blood-cells-in-haemostasis-and-thrombosis
#7
Rustem I Litvinov, John W Weisel
In contrast to an obsolete notion that erythrocytes, or red blood cells (RBCs), play a passive and minor role in hemostasis and thrombosis, over the past decades there has been increasing evidence that RBCs have biologically and clinically important functions in blood clotting and its disorders. This review summarizes the main mechanisms that underlie the involvement of RBCs in hemostasis and thrombosis in vivo, such as rheological effects on blood viscosity and platelet margination, aggregation and deformability of RBCs; direct adhesion and indirect biochemical interactions with endothelial cells and platelets, etc...
February 2017: ISBT Science Series
https://www.readbyqxmd.com/read/28453522/potential-biological-pathways-linking-type-d-personality-and-poor-health-a-cross-sectional-investigation
#8
Vera K Jandackova, Julian Koenig, Marc N Jarczok, Joachim E Fischer, Julian F Thayer
BACKGROUND: Type-D personality, defined as a combination of high negative affect and high social isolation, has been associated with poor health outcomes. However, pathways underlying this association are largely unknown. We investigated the relationship between Type-D personality and several biological and behavioral pathways including the autonomic nervous system, the immune system, glucose regulation and sleep in a large, apparently healthy sample. METHODS: Data from a total of 646 respondents (age 41...
2017: PloS One
https://www.readbyqxmd.com/read/28449641/cell-free-hemoglobin-mediated-oxidative-stress-is-associated-with-acute-kidney-injury-and-renal-replacement-therapy-in-severe-falciparum-malaria-an-observational-study
#9
Katherine Plewes, Hugh W F Kingston, Aniruddha Ghose, Richard J Maude, M Trent Herdman, Stije J Leopold, Haruhiko Ishioka, Md Mahtab Uddin Hasan, Md Shafiul Haider, Shamsul Alam, Kim A Piera, Prakaykaew Charunwatthana, Kamolrat Silamut, Tsin W Yeo, Md Abul Faiz, Sue J Lee, Mavuto Mukaka, Gareth D H Turner, Nicholas M Anstey, L Jackson Roberts, Nicholas J White, Nicholas P J Day, Md Amir Hossain, Arjen M Dondorp
BACKGROUND: Intravascular hemolysis is an intrinsic feature of severe malaria pathophysiology but the pathogenic role of cell-free hemoglobin-mediated oxidative stress in severe malaria associated acute kidney injury (AKI) is unknown. METHODS: As part of a prospective observational study, enrolment plasma cell-free hemoglobin (CFH), lipid peroxidation markers (F2-isoprostanes (F2-IsoPs) and isofurans (IsoFs)), red cell deformability, and serum creatinine were quantified in Bangladeshi patients with severe falciparum malaria (n = 107), uncomplicated malaria (n = 80) and sepsis (n = 28)...
April 27, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28446291/-clinical-significance-of-detecting-cd4-t-cells-in-peripheral-blood-of-patients-with-follicular-lymphoma
#10
Wei Liu, Guo-Xiang Zhagn, Wen-Zhi Shi, Lu Dong, Hua Liu, Xu-Liang Shen
OBJECTIVE: To investigate the changes of CD4(+) T lymphocytes in peripheral blood of patients with follicular lymphoma and its clinical significance. METHODS: Blood samples were collected for detection of whole blood cells, including absolute monocyte count (AMC), absolute lymphocyte count (ALC), hemoglobin (Hb), platelet count (Plt). Age, sex, pathological grade, number of involved lymph nodes, bone marrow involvement (BMI), Ann Arbor stage, B symptoms, serum lactate dehydrogenase (LDH) and serum β-2 microglobulin (β2-MG) were recorded, the prognostic stratification was performed by using FLIPI and FLIPI-2...
April 2017: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/28433992/the-aachen-minipig-phenotype-genotype-hematological-and-biochemical-characterization-and-comparison-to-the-g%C3%A3-ttingen-minipig
#11
Kerstin Pawlowsky, Lisa Ernst, Julia Steitz, Thaddäus Stopinski, Babette Kögel, Anna Henger, Reinhart Kluge, René Tolba
BACKGROUND: The pig is one of the most frequently used large animal models for biomedical research, especially in the field of translational research and surgical models. While standard livestock breeds are used in short-term and acute studies, minipig breeds are the preferred breeds in long-term and chronic studies due to their limited growth and body weight. OBJECTIVE: In consideration of the 3R principle (refinement, reduction, replacement) and the increasing demand, the aim of this study was to generate a new, robust, non-specific-pathogen-free minipig breed, the Aachen minipig...
April 22, 2017: European Surgical Research. Europäische Chirurgische Forschung. Recherches Chirurgicales Européennes
https://www.readbyqxmd.com/read/28428134/use-of-physiological-biomarkers-in-diagnosis-along-with-field-trials-of-different-trypanisidal-drugs-in-camels-of-cholistan-desert
#12
Aneela Zameer Durrani, Zubair Bashir, Khalid Mehmood, Muhammad Avais, Haroon Akbar, Waqas Ahmad, Muhammad Azeem
The point prevalence of trypanosomiasis with different physiological biomarkers along with evaluation of the most responsive trypanosidal drug against trypanosomiasis under field conditions was studied. For this purpose a total of 300 free range camels were selected at different grazing and watering point in Cholistan desert. The study population of camels included 150 clinically suspected camels for trypanosomiasis and 150 healthy camels with normal temperature, pulse and respiration. For therapeutic trials 36 positively diagnosed animals were randomly divided into three experimental groups for therapeutic trials...
April 18, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28423742/novel-potential-predictive-markers-of-sunitinib-outcomes-in-long-term-responders-versus-primary-refractory-patients-with-metastatic-clear-cell-renal-cell-carcinoma
#13
Javier Puente, Nuria Laínez, Marta Dueñas, María José Méndez-Vidal, Emilio Esteban, Daniel Castellano, Mónica Martinez-Fernández, Laura Basterretxea, María José Juan-Fita, Luis Antón, Luis León, Julio Lambea, Begoña Pérez-Valderrama, Sergio Vázquez, Cristina Suarez, Xavier Garcia Del Muro, Enrique Gallardo, José Pablo Maroto, M Luz Samaniego, Beatriz Suárez-Paniagua, Julián Sanz, Jesús M Paramio
BACKGROUND: Several potential predictive markers of efficacy of targeted agents in patients with metastatic renal cell carcinoma (mRCC) have been identified. Interindividual heterogeneity warrants further investigation. PATIENTS AND METHODS: Multicenter, observational, retrospective study in patients with clear-cell mRCC treated with sunitinib. Patients were classified in two groups: long-term responders (LR) (progression-free survival (PFS)≥22 months and at least stable disease), and primary refractory (PR) (progressive disease within 3-months of sunitinib onset)...
May 2, 2017: Oncotarget
https://www.readbyqxmd.com/read/28420988/heme-as-a-target-for-therapeutic-interventions
#14
REVIEW
Stephan Immenschuh, Vijith Vijayan, Sabina Janciauskiene, Faikah Gueler
Heme is a complex of iron and the tetrapyrrole protoporphyrin IX with essential functions in aerobic organisms. Heme is the prosthetic group of hemoproteins such as hemoglobin and myoglobin, which are crucial for reversible oxygen binding and transport. By contrast, high levels of free heme, which may occur in various pathophysiological conditions, are toxic via pro-oxidant, pro-inflammatory and cytotoxic effects. The toxicity of heme plays a major role for the pathogenesis of prototypical hemolytic disorders including sickle cell disease and malaria...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28411071/predictive-nomogram-for-recurrence-following-surgery-for-non-metastatic-renal-cell-cancer-with-tumor-thrombus
#15
E Jason Abel, Timothy A Masterson, Jose A Karam, Viraj A Master, Vitaly Margulis, Ryan Hutchinson, C Adam Lorentz, Evan Bloom, Tyler M Bauman, Christopher G Wood, Michael L Blute
BACKGROUND: Following surgery for non-metastatic renal cell carcinoma (RCC) with tumor thrombus, the risk of recurrence is significant but variable among individual patients. The purpose of this study is to develop and validate a predictive nomogram for individual estimation of recurrence risk following surgery for RCC with venous tumor thrombus. METHODS: Comprehensive data was collected for non-metastatic RCC patients with thrombus treated at 5 institutions from 2000-2013...
April 11, 2017: Journal of Urology
https://www.readbyqxmd.com/read/28404933/aurora-a-affects-radiosenstivity-in-cervical-squamous-cell-carcinoma-and-predicts-poor-prognosis
#16
Yuhua Ma, Jie Yang, Ruozheng Wang, Zegao Zhang, Xiaoli Qi, Chunhua Liu, Miaomiao Ma
BACKGROUND: Definitive radiation therapy (RT) (with or without cisplatin-based chemotherapy) is one of the most effective treatments for cervical squamous cell carcinoma (CSCC), but efficacy is limited due to resistance. In the present study, we investigated the relationship between the expression of Aurora kinase A (Aurora-A, AURKA)and response to RT in patients with CSCC. METHODS: The expression of Aurora-A in biopsy specimens of untreated primary tumors in 129 Uyghur patients with CSCC was investigated immunohistochemically...
May 9, 2017: Oncotarget
https://www.readbyqxmd.com/read/28398071/raman-spectroscopy-of-blood-and-blood-components
#17
Chad G Atkins, Kevin Buckley, Michael W Blades, Robin F B Turner
Blood is a bodily fluid that is vital for a number of life functions in animals. To a first approximation, blood is a mildly alkaline aqueous fluid (plasma) in which a large number of free-floating red cells (erythrocytes), white cells (leucocytes), and platelets are suspended. The primary function of blood is to transport oxygen from the lungs to all the cells of the body and move carbon dioxide in the return direction after it is produced by the cells' metabolism. Blood also carries nutrients to the cells and brings waste products to the liver and kidneys...
January 1, 2017: Applied Spectroscopy
https://www.readbyqxmd.com/read/28396834/toxic-effects-of-dimethyl-sulfoxide-on-red-blood-cells-platelets-and-vascular-endothelial-cells-in-vitro
#18
Xiaoyang Yi, Minxia Liu, Qun Luo, Hailong Zhuo, Hui Cao, Jiexi Wang, Ying Han
Dimethyl sulfoxide (DMSO) is widely used in biological studies as a cryoprotective agent for cells and tissues, and also for cryopreserved platelets (PLTs). However, few data on the toxic effects of DMSO following intravenous infusion of cryopreserved PLTs are available. The aim of this study was to explore dose-related effects of DMSO on red blood cells (RBCs), PLTs and vascular endothelial cells in vitro. The results showed that DMSO treatments had significant effects on RBCs, affecting osmotic fragility and increasing hemolysis...
April 2017: FEBS Open Bio
https://www.readbyqxmd.com/read/28385576/blood-transfusions-may-impair-endothelium-dependent-vasodilatation-during-coronary-artery-bypass-surgery
#19
Alessio Rungatscher, Elisabetta Milani, Cecilia Covajes, Seth Hallström, Leonardo Gottin, Gian Cesare Guidi, Giovanni Battista Luciani, Giuseppe Faggian
OBJECTIVE: The hemolytic product free-hemoglobin (fHb) reduces nitric oxide (NO) bioavailability. The present study aims to establish whether administration of different blood transfusions result in increased circulating fHb levels and NO consumption with effects on arterial NO-dependent blood flow in patients undergoing CABG surgery. METHODS: Ninety-five consecutive patients undergoing elective CABG surgery were prospectively divided in four groups based on blood transfusion requirements during surgery: stored blood cells (SBC, n...
July 2017: Microvascular Research
https://www.readbyqxmd.com/read/28379307/characteristics-outcomes-prognostic-factors-and-treatment-of-patients-with-t-cell-prolymphocytic-leukemia-t-pll
#20
P Jain, E Aoki, M Keating, W G Wierda, S O'Brien, G N Gonzalez, A Ferrajoli, N Jain, P A Thompson, E Jabbour, R K Shamanna, S Pierce, A Alousi, C Hosing, I Khouri, Z Estrov, J Cortes, H Kantarjian, F Ravandi, T M Kadia
Background: T-cell prolymphocytic leukemia (T-PLL) is a rare and aggressive disease. In this study, we report our experience from 119 patients with T-PLL. Patients and Methods: We reviewed the clinico-pathologic records of 119 consecutive patients with T-PLL, who presented to our institution between 1990 and 2016. Results: One hundred and nineteen patients with T-PLL were analysed. Complex karyotype and aberrations in chromosome 14 were seen in 65% and 52% patients, respectively...
April 4, 2017: Annals of Oncology: Official Journal of the European Society for Medical Oncology
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