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https://www.readbyqxmd.com/read/28316625/microbiological-study-of-cast-posts-before-cementation
#1
Maricela Vallejo-Labrada, Juan Carlos Ojeda-Garces
This study identifies the most common microorganisms present in type III gold cast posts related to pulpal disease and evaluates the sterilization/disinfection method before cementation in the root canal. Forty-five type III gold cast posts were aseptically collected in sterile sealed plastic bags and taken to the microbiology laboratory to carry out the study: fifteen cast posts had no treatment, fifteen were disinfected (immersion in 70% alcohol during 15 minutes), and fifteen were autoclaved at 121°C for 15 minutes by using saturated steam under 15 psi pressure...
2017: International Journal of Dentistry
https://www.readbyqxmd.com/read/28290315/inactivation-modeling-of-human-enteric-virus-surrogates-ms2-q%C3%AE-and-%C3%AE-x174-in-water-using-uvc-leds-a-novel-disinfecting-system
#2
Do-Kyun Kim, Soo-Ji Kim, Dong-Hyun Kang
In order to assure the microbial safety of drinking water, UVC-LED treatment has emerged as a possible technology to replace the use of conventional low pressure (LP) mercury vapor UV lamps. In this investigation, inactivation of Human Enteric Virus (HuEV) surrogates with UVC-LEDs was investigated in a water disinfection system, and kinetic model equations were applied to depict the surviving infectivities of the viruses. MS2, Qβ, and ΦX 174 bacteriophages were inoculated into sterile distilled water (DW) and irradiated with UVC-LED printed circuit boards (PCBs) (266nm and 279nm) or conventional LP lamps...
January 2017: Food Research International
https://www.readbyqxmd.com/read/28268388/effects-of-a-non-thermal-plasma-needle-device-on-hpv-16-positive-cervical-cancer-cell-viability-in-vitro
#3
Hollie A Ryan, Johanna Neuber, Shutong Song, Stephen J Beebe, Chunqi Jiang
Non-thermal atmospheric pressure plasma has been demonstrated to inactivate a wide range of surface-dwelling pathogenic microorganisms including airborne viral particles, vegetative bacteria and bacterial spores. This shows the promise of plasma-based decontamination procedures for broad-spectrum sterilization and disinfection and promotes the in-depth and systematic study of how plasma treatment conditions relate to pathogen inactivation efficiency. A wide knowledge gap nonetheless exists regarding whether certain plasma parameters and exposure conditions can alter the resistance of virus-linked cancer cells to treatment...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28258267/evaluation-of-4-sodium-hypochlorite-in-eliminating-enterococcus-faecalis-from-the-root-canal-when-used-with-three-irrigation-methods-an-in-vitro-study
#4
Harsh Priyank, Vinisha Pandey, Abhishek Bagul, Kishore Kumar Majety, Parul Verma, Basanta Kumar Choudhury
INTRODUCTION: Endodontic treatment removes all pathogens, such as Enterococcus faecalis from pulp and root canals. The aim of this study is to assess the usefulness of sodium hypo-chlorite (NaOCl) in removing E. faecalis from the root canal used with three different irrigation methods. MATERIALS AND METHODS: This study was conducted on freshly extracted maxillary incisors. After biomechanical preparation, root canals were injected with E. faecalis. Three groups were made which contained 30 teeth in each group; 2 mL of NaOCl solution was used for irrigation followed by agitation with K-files in group I; 2 mL of NaOCl solution was used for irrigation and ultrasonic agitation was done in group II...
March 1, 2017: Journal of Contemporary Dental Practice
https://www.readbyqxmd.com/read/28253181/microwave-radiation-is-effective-at-disinfecting-dental-stone-surfaces-without-changing-their-physical-properties
#5
Ariel José Bona, Mauro Gustavo Amaral-Brito, José Augusto Rodrigues, Daiane Cristina Peruzzo, Fabiana Mantovani Gomes França
The aims of this study were to evaluate the effectiveness of different microwave radiation regimens for disinfection of type IV dental stone surfaces and to assess the influence of these regimens on surface roughness and dimensional change following disinfection. Three hundred cylindrical (20 × 2-mm) test specimens were made in type IV stone and divided into subgroups of 20 according to the microorganisms tested (Staphylococcus aureus, Escherichia coli, or Candida albicans) and the 900-W microwave radiation protocol (cycles of 3, 5, or 7 minutes; a positive control; or a negative control)...
March 2017: General Dentistry
https://www.readbyqxmd.com/read/28249746/the-evaluation-of-cultivable-microbiota-profile-in-patients-with-secondary-endodontic-infection-before-and-after-photo-activated-disinfection
#6
Maryam Pourhajibagher, Roghayeh Ghorbanzadeh, Steven Parker, Nasim Chiniforush, Abbas Bahador
BACKGROUND: Secondary/persistent endodontic infection can be the outcome of failure of endodontic treatment. Photo-activated disinfection (PAD) can be a useful adjunct to mechanical and antimicrobial agents in eliminating endopathogenic microorganisms. In this study, we evaluated the effect of PAD on diversity and count of microbiota related to secondary/persistent endodontic infections. MATERIALS AND METHODS: Root canal samples were taken using sterile paper points from the root canals of 14 patients with secondary/persistent endodontic infections after removing the root-canal filling materials...
February 26, 2017: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/28244579/prevention-of-healthcare-associated-infections-a-descriptive-study
#7
R Accardi, S Castaldi, A Marzullo, S Ronchi, D Laquintana, M Lusignani
BACKGROUND: This study aims to verify whether there are, and to which degree, knowledge and adherence to guidelines on the prevention and control of healthcare associated infections by nursing staff. Study design. A descriptive study was conducted on a sample of nurses in the areas of medicine, surgery, and its own specialties of the Istituto di Ricovero e Cura a Carattere Scientifico Ca' Granda Ospedale Maggiore Policlinico in Milan from 1st December 2015 to 29th February 2016. METHODS: The knowledge of the nursing staff have been investigated through the use of questionnaires with anonymous self-reporting method; inspections in the wards using observational grids were carried out in order to verify adherence to best-practice principles...
March 2017: Annali di Igiene: Medicina Preventiva e di Comunità
https://www.readbyqxmd.com/read/28241955/clinical-issues-march-2017
#8
Sharon A Van Wicklin
Using one flexible endoscope for an upper and lower gastrointestinal (GI) endoscopy procedure Key words: flexible endoscope, esophagogastroduodenoscopy, colonoscopy, enteroscope, risk assessment. Enhanced methods for processing flexible duodenoscopes Key words: flexible duodenoscopes, liquid chemical sterilization, ethylene oxide sterilization, low-temperature sterilization, risk assessment. Modification of the Spaulding classification Key words: Spaulding classification, critical items, semicritical items, mucous membranes, risk assessment...
March 2017: AORN Journal
https://www.readbyqxmd.com/read/28241951/back-to-basics-instrument-cleaning
#9
Lisa Spruce
Adequately cleaning and processing surgical instruments may be challenging for perioperative team members; however, the cleaning and processing of instruments are critical steps in making instruments safe to use on patients and achieving an appropriately safe OR environment. Instruments that are cleaned properly have had organic debris and soil removed, rendering them ready for sterilization or disinfection. This Back to Basics article covers the basic steps and considerations for cleaning and processing instruments before sterilization...
March 2017: AORN Journal
https://www.readbyqxmd.com/read/28226554/effects-of-a-non-thermal-plasma-needle-device-on-hpv-16-positive-cervical-cancer-cell-viability-in-vitro
#10
Hollie A Ryan, Johanna Neuber, Shutong Song, Stephen J Beebe, Chunqi Jiang, Hollie A Ryan, Johanna Neuber, Shutong Song, Stephen J Beebe, Chunqi Jiang, Shutong Song, Johanna Neuber, Chunqi Jiang, Stephen J Beebe, Hollie A Ryan
Non-thermal atmospheric pressure plasma has been demonstrated to inactivate a wide range of surface-dwelling pathogenic microorganisms including airborne viral particles, vegetative bacteria and bacterial spores. This shows the promise of plasma-based decontamination procedures for broad-spectrum sterilization and disinfection and promotes the in-depth and systematic study of how plasma treatment conditions relate to pathogen inactivation efficiency. A wide knowledge gap nonetheless exists regarding whether certain plasma parameters and exposure conditions can alter the resistance of virus-linked cancer cells to treatment...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28214143/retrospective-study-of-candida-sp-contaminations-of-endoscopes-at-the-university-hospital-of-tlemcen-algeria
#11
REVIEW
I Hassaine-Lahfa, Z Boucherit-Otmani, L Sari-Belkherroubi, K Boucherit
BACKGROUND: Improper cleaning and disinfection of endoscopes has been responsible for multiple nosocomial outbreaks and sometimes serious life-threatening infections. OBJECTIVE: The aim of our study is, at first, to identify Candida species responsible for the contamination of endoscopes, and to determine the minimal inhibitory concentrations of planktonic (MIC) and sessile cells (SMIC) of amphotericin B (AmB) against our isolated strains. MATERIALS AND METHODS: The present study was performed on four endoscopes in the department of gastroenterology at the University Hospital of Tlemcen (Algeria)...
February 14, 2017: Journal de Mycologie Médicale
https://www.readbyqxmd.com/read/28186464/evaluation-of-antimicrobial-photodynamic-therapy-using-indocyanine-green-and-near-infrared-diode-laser-against-enterococcus-faecalis-in-infected-human-root-canals
#12
Charis Beltes, Nikolaos Economides, Hercules Sakkas, Chrissanthy Papadopoulou, Theodoros Lambrianidis
OBJECTIVE: Photodynamic therapy (PDT) is considered a promising adjunct to the currently available endodontic disinfection techniques leading to more effective reduction of intracanal bacteria. The present ex vivo study aimed to assess the antimicrobial effect of PDT using indocyanine green (ICG) as photosensitizer and a near-infrared (NIR) diode laser in root canals of human teeth infected with Enterococcus faecalis. MATERIALS AND METHODS: Ninety single-rooted teeth after chemomechanical preparation and sterilization were contaminated with an E...
February 9, 2017: Photomedicine and Laser Surgery
https://www.readbyqxmd.com/read/28174366/radiofrequency-glow-discharge-as-a-mode-of-disinfection-for-elastomeric-impression-materials
#13
Susan Mathew, Mathew M Alani, K N Velayudhan Nair, Sayij Haridas, P B Reba, Sherin A Thomas
INTRODUCTION: Prosthodontic practice involves procedures in which impressions of the maxillary and mandibular arches are mandatory. Cross infection is one of the major problems that can occur in regular dental practice. Every dentist should take utmost care to prevent cross infection as oral cavity is the source of variety of microorganisms which can often cause diseases that can be fatal. Although precautions, such as wearing of gloves and mask, sterilization of instruments are given importance, the need for disinfection of impressions is often neglected...
February 1, 2017: Journal of Contemporary Dental Practice
https://www.readbyqxmd.com/read/28146555/disinfection-of-ebola-virus-in-sterilized-municipal-wastewater
#14
Kyle Bibby, Robert J Fischer, Leonard W Casson, Nathalia Aquino de Carvalho, Charles N Haas, Vincent J Munster
Concerns have been raised regarding handling of Ebola virus contaminated wastewater, as well as the adequacy of proposed disinfection approaches. In the current study, we investigate the inactivation of Ebola virus in sterilized domestic wastewater utilizing sodium hypochlorite addition and pH adjustment. No viral inactivation was observed in the one-hour tests without sodium hypochlorite addition or pH adjustment. No virus was recovered after 20 seconds (i.e. 4.2 log10 unit inactivation to detection limit) following the addition of 5 and 10 mg L-1 sodium hypochlorite, which resulted in immediate free chlorine residuals of 0...
February 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28134720/multidepartmental-response-to-a-duodenoscope-used-on-a-cre-patient-a-case-study
#15
Mark A Bruns, Elizabeth R Gibbs
Transmission of carbapenem-resistant Enterobacteriaceae (CRE) via duodenoscopes, specialized endoscopes used during endoscopic retrograde cholangiopancreatography (ERCP) procedures, has attracted media attention since early 2015. This attention has placed increasing focus on the reprocessing of duodenoscopes. Current reprocessing recommendations for these endoscopes require either high-level disinfection or ethylene oxide sterilization. While reprocessing duodenoscopes, staff at endoscopy locations within the Mercy health system perform a single high-level disinfection cycle that is preceded by two cycles of manual cleaning...
January 2017: Gastroenterology Nursing: the Official Journal of the Society of Gastroenterology Nurses and Associates
https://www.readbyqxmd.com/read/28077517/two-phase-hospital-associated-outbreak-of-mycobacterium-abscessus-investigation-and-mitigation
#16
Arthur W Baker, Sarah S Lewis, Barbara D Alexander, Luke F Chen, Richard J Wallace, Barbara A Brown-Elliott, Pamela J Isaacs, Lisa C Pickett, Chetan B Patel, Peter K Smith, John M Reynolds, Jill Engel, Cameron R Wolfe, Carmelo A Milano, Jacob N Schroder, Robert D Davis, Matthew G Hartwig, Jason E Stout, Nancy Strittholt, Eileen K Maziarz, Jennifer Horan Saullo, Kevin C Hazen, Richard J Walczak, Ravikiran Vasireddy, Sruthi Vasireddy, Celeste M McKnight, Deverick J Anderson, Daniel J Sexton
BACKGROUND: Nontuberculous mycobacteria (NTM) commonly colonize municipal water and cause healthcare-associated outbreaks. We investigated a biphasic outbreak of Mycobacterium abscessus at a tertiary care hospital. METHODS: Case patients had recent hospital exposure and laboratory-confirmed colonization or infection with M. abscessus from January 2013 through December 2015. We conducted a multidisciplinary epidemiologic, field, and laboratory investigation. RESULTS: The incidence rate of M...
January 11, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/28050984/evaluation-of-bacteriological-profile-in-the-apical-root-segment-of-the-patients-with-primary-apical-periodontitis
#17
Aravind Tatikonda, N Sudheep, Krishna P Biswas, K Gowtham, Sudarshan Pujari, Padam Singh
INTRODUCTION: Apical periodontitis usually results from bacterial accumulation and contamination occurring in the root-canal system, and extending beyond the apical foramen to involve the periapical tissues. Literature has a paucity of the studies that stress on the division and analysis of the pulp canal segments. The reason for this disparity might be the technique used for collecting the samples from the pulp canals. Hence, we carried out the present study to evaluate the microbial flora in the apical part of the roots with necrotic pulp canals...
January 1, 2017: Journal of Contemporary Dental Practice
https://www.readbyqxmd.com/read/28045704/neonatal-peripherally-inserted-central-catheter-practices-and-providers-results-from-the-neonatal-picc1-survey
#18
Elizabeth Sharpe, Latoya Kuhn, David Ratz, Sarah L Krein, Vineet Chopra
BACKGROUND: Neonatal intensive care units (NICUs) commonly utilize peripherally inserted central catheters (PICCs) to provide nutrition and long-term medications to premature and full-term infants. However, little is known about PICC practices in these settings. PURPOSE: To assess PICC practices, policies, and providers in NICUs. METHODS: The Neonatal PICC1 Survey was conducted through the use of the electronic mailing list of a national neonatal professional organization's electronic membership community...
December 29, 2016: Advances in Neonatal Care: Official Journal of the National Association of Neonatal Nurses
https://www.readbyqxmd.com/read/28040502/a-comparison-between-effect-of-photodynamic-therapy-by-led-and-calcium-hydroxide-therapy-for-root-canal-disinfection-against-enterococcus-faecalis-a-randomized-controlled-trial
#19
Mohammad Asnaashari, Hengameh Ashraf, Afsaneh Rahmati, Neda Amini
BACKGROUND: Insufficient root canal disinfection is one of the main reasons for persistent periapical pathology. Photodynamic therapy (PDT) has been proven effective in disinfecting infected root canals. The aim of this study was to evaluate the antimicrobial effect of photo activated disinfection (PAD) when using toluidine blue as photosensitizer and a LED lamp after the conventional treatment, and comparing it with calcium hydroxide therapy in vivo. METHODS: This clinical trial includes 20 patients with molars requiring endodontic retreatment...
December 28, 2016: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/27996305/infection-control-and-patient-discomfort-with-an-alternative-plastic-barrier-in-intraoral-digital-radiography
#20
Arnon Charuakkra, Sangsom Prapayasatok, Apirum Janhom, Karune Verochana, Phattaranant Mahasantipiya
OBJECTIVES: (1) To compare the efficacy of a commercially available hygienic sheath and an alternative plastic bag in preventing contamination of the imaging plate during intraoral radiography and (2) to compare patient discomfort when using the hygienic sheath and the plastic bag. METHODS: 60 sterilized Size 2 imaging plates covered with either the hygienic sheath (n = 30) or the plastic bag (n = 30) were used to simulate digital periapical radiographic examination in 30 volunteer patients...
February 2017: Dento Maxillo Facial Radiology
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