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Sterilization and disinfection

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https://www.readbyqxmd.com/read/28440891/evaluation-of-three-different-decontamination-techniques-on-biofilm-formation-and-on-physical-and-chemical-properties-of-resin-composites
#1
Carolina Bosso André, Andressa Dos Santos, Carmem Silvia Pfeifer, Marcelo Giannini, Emerson Marcelo Girotto, Jack Liborio Ferracane
OBJECTIVES: This study evaluated three different sterilization/disinfection techniques for resin composites on bacterial growth and surface modification after decontamination. METHODS: Two resin composites were sterilized/disinfected with three different techniques: UV light, 1% chloramine T, and 70% ethanol. Four different times were used for each technique to determine the shortest time that the solution or UV light was effective. The influence of sterilization/disinfection technique on bacterial growth was evaluated by analyzing the metabolic activity, using the AlamarBlue™ assay, bacterial viability, and SEM images from biofilms of Streptococcus mutans...
April 25, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28434755/-evaluation-of-the-operating-results-and-costs-associated-with-the-implementation-of-a-flexible-ureteroscopy-activity-within-a-university-hospital-center
#2
K Del Santo, M Audouin, I Ouzaid, V Ravery, O Traxer
INTRODUCTION: The increasing use of flexible ureteroscopy (USSR) sets the cost problems inevitably associated with the fragility of these endoscopes. The aim of this work is to clear from a recent example (universitary hospital) results, costs during the implementation of this technique. MATERIAL AND METHODS: This is a retrospective study conducted from December 2012 (date of introduction of the activity) in March 2015 concerning the USSR made in a French universitary hospital for stone disease...
April 20, 2017: Progrès en Urologie
https://www.readbyqxmd.com/read/28431851/ultraviolet-c-light-as-a-means-of-disinfecting-anesthesia-workstations
#3
Matthew Nottingham, Gene Peterson, Christopher Doern, Michelle Doll, Nadia Masroor, Kakotan Sanogo, Michael Stevens, Gonzalo Bearman
BACKGROUND: Anesthesia workstations (AWs) are a reservoir for pathogenic organisms potentially associated with surgical site infections. This study examined the effectiveness of the Tru-D SmartUVC device (Tru-D LLC, Nashville, TN) on bioburden reduction (BR) on AWs. METHODS: Strips of tissue inoculated with a known concentration of either Staphylococcus aureus, Enterococcus faecalis, or Acinetobacter sp were placed on 22 high-touch surfaces of an AW. Half of the AW surfaces received direct ultraviolet (UV) light exposure and half received indirect exposure...
April 18, 2017: American Journal of Infection Control
https://www.readbyqxmd.com/read/28426292/efficacy-of-three-different-lasers-on-eradication-of-enterococcus-faecalis-and-candida-albicans-biofilms-in-root-canal-system
#4
Silvija Kasić, Marita Knezović, Nataša Beader, Dragana Gabrić, Ana Ivanišević Malčić, Anja Baraba
OBJECTIVE: The objective was to compare the efficacy of three different lasers in disinfection of root canals inoculated with Enterococcus faecalis and Candida albicans biofilms. BACKGROUND: Endodontic space disinfection depends on the type of root canal irrigant used and the way it is delivered and agitated because irrigants have limited ability to reach all parts of root canal system. MATERIALS AND METHODS: Thirty single-rooted human teeth were selected...
April 20, 2017: Photomedicine and Laser Surgery
https://www.readbyqxmd.com/read/28422353/comprehensive-review-and-comparison-of-the-disinfection-techniques-currently-available-in-the-literature
#5
REVIEW
Ahila S Chidambaranathan, Muthukumar Balasubramanium
The goal of disinfection in the dental office is to prevent the spread of infection from one patient to another and maintain the safety of the dental care providers. Prevention of cross infection has significant effect on infection control. The standard procedure of rinsing impressions under tap water immediately after removal from the mouth eliminates microorganisms along with saliva and blood. A broad search on published literature was done using the keywords impression materials, disinfection method, and sterilization of dental impression from 1980 to 2016 in Medline, Google Scholar, the internet, and textbooks...
April 19, 2017: Journal of Prosthodontics: Official Journal of the American College of Prosthodontists
https://www.readbyqxmd.com/read/28396277/duodenoscope-reprocessing-surveillance-with-adenosine-triphosphate-testing-and-terminal-cultures-a-clinical-pilot-study
#6
Kavel Visrodia, Yuri Hanada, Kelly M Pennington, Pritish K Tosh, Mark D Topazian, Bret T Petersen
BACKGROUND & AIMS: Recent reports of infectious outbreaks linked to duodenoscopes have led to proposals for duodenoscope surveillance culturing, which has inherent limitations. We aimed to assess the feasibility of real-time adenosine triphosphate (ATP) testing after manual cleaning, and its ability to predict reprocessing adequacy, as determined by terminal duodenoscope cultures. METHODS: Clinically used duodenoscopes underwent reprocessing per current guidelines...
April 7, 2017: Gastrointestinal Endoscopy
https://www.readbyqxmd.com/read/28379193/a-force-visualized-silicone-retractor-attachable-to-surgical-suction-pipes
#7
Tetsuyou Watanabe, Toshio Koyama, Takeshi Yoneyama, Mitsutoshi Nakada
This paper presents a force-visually-observable silicone retractor, which is an extension of a previously developed system that had the same functions of retracting, suction, and force sensing. These features provide not only high usability by reducing the number of tool changes, but also a safe choice of retracting by visualized force information. Suction is achieved by attaching the retractor to a suction pipe. The retractor has a deformable sensing component including a hole filled with a liquid. The hole is connected to an outer tube, and the liquid level displaced in proportion to the extent of deformation resulting from the retracting load...
April 5, 2017: Sensors
https://www.readbyqxmd.com/read/28377648/comparison-of-antimicrobial-efficacy-of-diode-laser-triphala-and-sodium-hypochlorite-in-primary-root-canals-a-randomized-controlled-trial
#8
Seby Thomas, Sharath Asokan, Baby John, Geetha Priya, S Kumar
AIM: To evaluate the antimicrobial efficacy of diode laser, triphala, and sodium hypochlorite (NaOCl) against Enterococc-cus faecalis contaminated primary root canals. MATERIALS AND METHODS: Forty-nine single-rooted human primary teeth were reduced up to cemento-enamel junction and biomechanically prepared. After sterilization, five teeth were selected as negative controls and remaining teeth were inoculated with E. faecalis. The teeth were then randomly divided into four groups...
January 2017: International Journal of Clinical Pediatric Dentistry
https://www.readbyqxmd.com/read/28375579/seed-vectored-endophytic-bacteria-modulate-development-of-rice-seedlings
#9
Satish Kumar Verma, Kathryn Kingsley, Ivelisse Irizarry, Marshall Bergen, Ravindra Nath Kharwar, James F White
AIM: The aim of the present study was to evaluate the effects of the removal of indigenous bacteria from rice seeds on seedling growth and development. Here we report the presence of three indigenous endophytic bacteria in rice seeds that play important roles in modulating seedling development (shoot and root lengths, and formation of root hairs and secondary roots) and defense against pathogens. METHODS AND RESULTS: Seed associated bacteria were removed using surface sterilization with NaOCl (bleach) followed by antibiotic treatment...
April 4, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28372421/an-all-solid-state-microsecond-range-quasi-square-pulse-generator-based-on-fractional-turn-ratio-saturable-pulse-transformer-and-anti-resonance-network
#10
Rong Chen, Jianhua Yang, Xinbing Cheng, Zilong Pan
High voltage pulse generators are widely applied in a number of fields. Defense and industrial applications stimulated intense interests in the area of pulsed power technology towards the system with high power, high repetition rate, solid state characteristics, and compact structure. An all-solid-state microsecond-range quasi-square pulse generator based on a fractional-turn ratio saturable pulse transformer and anti-resonance network is proposed in this paper. This generator consists of a charging system, a step-up system, and a modulating system...
March 2017: Review of Scientific Instruments
https://www.readbyqxmd.com/read/28364327/assessment-of-chlorine-tolerance-profile-of-citrobacter-species-recovered-from-wastewater-treatment-plants-in-eastern-cape-south-africa
#11
Mojisola Owoseni, Anthony Okoh
This present study assessed the chlorine tolerance of some Citrobacter species recovered from secondary effluents from the clarifiers of two wastewater treatment plants in the Eastern Cape, South Africa. The bacterial survival, chlorine lethal dose and inactivation kinetics at lethal doses were examined. Inactivation of the test bacteria (n = 20) at the recommended dose of 0.5 mg/l for 30 min exposure showed a progressive reduction in bacterial population from 4 to 5 log reduction and residuals ranged between 0...
April 2017: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/28364108/-bactericidal-effects-and-smear-layer-removal-of-er-yag-laser-radiation-against-enterococcus-faecalis-in-root-canals
#12
Zongbo Li, Changyun Fang
To evaluate the cleaning ability of Er:YAG laser against Enterococcus faecalis in root canals.
 Methods: The single-rooted human teeth were sterilized and inoculated with Enterococcus faecalis and were randomly assigned into 2 groups. A group of teeth was irrigated with saline during root canal preparation while another group with NaClO. After mechanical preparation, the two groups were randomly divided into 3 subgroups according to the different treatments: Samples, laser radiation, and calcium hydroxide intracanal medication for 7 days...
March 28, 2017: Zhong Nan da Xue Xue Bao. Yi Xue Ban, Journal of Central South University. Medical Sciences
https://www.readbyqxmd.com/read/28362371/a-device-for-performing-cell-migration-wound-healing-in-a-96-well-plate
#13
Po Ying Poon, Patrick Ying Kit Yue, Ricky Ngok Shun Wong
The cell migration/wounding assay is a commonly used method to study cell migration and other biological processes, such as angiogenesis and tumor metastasis. In this assay, cells are grown to form a confluent monolayer and a mechanical wound is created by scratching with a device. Then the migration rate of the cells towards the denuded area can be monitored by imaging. Our 8-channel mechanical wounder is designed to tackle most of the problems associated with the cell migration assay. Firstly, our wounder can be easily sterilized by autoclaving or with common disinfectants...
March 7, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28361272/sterilization-of-blow-fly-eggs-chrysomya-megacephala-and-lucilia-cuprina-diptera-calliphoridae-for-maggot-debridement-therapy-application
#14
Kwankamol Limsopatham, Phadungkiat Khamnoi, Kabkaew L Sukontason, Dheerawan Boonyawan, Tarinee Chaiwong, Kom Sukontason
Maggot debridement therapy (MDT) is an application of sterile laboratory-reared blow fly larvae to remove necrotic tissue and disinfect wounds for medical conditions. For effective application, the blow fly larvae used in the wound treatment are required to be in aseptic condition. Here, we report the results of a detailed assessment of bacteria and fungi isolated from the eggs of two blow fly species, Chrysomya megacephala (F.) and Lucilia cuprina (Wiedemann) before and after sterilization by disinfectants Chlorhex-C, povidone-iodine, and sodium hypochlorite...
March 30, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28360994/mrsa-decolonization-failure-are-biofilms-the-missing-link
#15
Frank Günther, Brigitte Blessing, Evelina Tacconelli, Nico T Mutters
BACKGROUND: Device-associated infections due to biofilm-producing methicillin-resistant Staphylococcus aureus (MRSA) have been recently associated with the failure of antibiotic treatment and decolonization measures. The goal of our study was to evaluate the extent to which the formation of biofilms influenced the efficacy of topical decolonization agents or disinfectants such as mupirocin (MUP), octenidine (OCT), chlorhexidine (CHG), polyhexanide (POL), and chloroxylenol (CLO). METHODS: Bacterial killing in biofilms by the disinfectants and MUP was determined as the reduction [%] in metabolic activity determined by a biofilm viability assay that uses kinetic analysis of metabolic activity...
2017: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/28351879/a-non-gas-based-cryotherapy-system-for-the-treatment-of-cervical-intraepithelial-neoplasia-a-mixed-methods-approach-for-initial-development-and-testing
#16
Miriam Cremer, Proma Paul, Katie Bergman, Michael Haas, Mauricio Maza, Albert Zevallos, Miguel Ossandon, Jillian D Garai, Jennifer L Winkler
BACKGROUND: Gas-based cryotherapy is the most widely used treatment strategy for cervical intraepithelial neoplasia (CIN) in low-resource settings, but reliance on gas presents challenges in low- and middle-income countries (LMICs). Our team adapted the original CryoPen Cryosurgical System, a cryotherapy device that does not require compressed gas and is powered by electricity, for use in LMICs. METHODS: A mixed-methods approach was used involving both qualitative and quantitative methods...
March 24, 2017: Global Health, Science and Practice
https://www.readbyqxmd.com/read/28316625/microbiological-study-of-cast-posts-before-cementation
#17
Maricela Vallejo-Labrada, Juan Carlos Ojeda-Garces
This study identifies the most common microorganisms present in type III gold cast posts related to pulpal disease and evaluates the sterilization/disinfection method before cementation in the root canal. Forty-five type III gold cast posts were aseptically collected in sterile sealed plastic bags and taken to the microbiology laboratory to carry out the study: fifteen cast posts had no treatment, fifteen were disinfected (immersion in 70% alcohol during 15 minutes), and fifteen were autoclaved at 121°C for 15 minutes by using saturated steam under 15 psi pressure...
2017: International Journal of Dentistry
https://www.readbyqxmd.com/read/28290315/inactivation-modeling-of-human-enteric-virus-surrogates-ms2-q%C3%AE-and-%C3%AE-x174-in-water-using-uvc-leds-a-novel-disinfecting-system
#18
Do-Kyun Kim, Soo-Ji Kim, Dong-Hyun Kang
In order to assure the microbial safety of drinking water, UVC-LED treatment has emerged as a possible technology to replace the use of conventional low pressure (LP) mercury vapor UV lamps. In this investigation, inactivation of Human Enteric Virus (HuEV) surrogates with UVC-LEDs was investigated in a water disinfection system, and kinetic model equations were applied to depict the surviving infectivities of the viruses. MS2, Qβ, and ΦX 174 bacteriophages were inoculated into sterile distilled water (DW) and irradiated with UVC-LED printed circuit boards (PCBs) (266nm and 279nm) or conventional LP lamps...
January 2017: Food Research International
https://www.readbyqxmd.com/read/28268388/effects-of-a-non-thermal-plasma-needle-device-on-hpv-16-positive-cervical-cancer-cell-viability-in-vitro
#19
Hollie A Ryan, Johanna Neuber, Shutong Song, Stephen J Beebe, Chunqi Jiang
Non-thermal atmospheric pressure plasma has been demonstrated to inactivate a wide range of surface-dwelling pathogenic microorganisms including airborne viral particles, vegetative bacteria and bacterial spores. This shows the promise of plasma-based decontamination procedures for broad-spectrum sterilization and disinfection and promotes the in-depth and systematic study of how plasma treatment conditions relate to pathogen inactivation efficiency. A wide knowledge gap nonetheless exists regarding whether certain plasma parameters and exposure conditions can alter the resistance of virus-linked cancer cells to treatment...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28258267/evaluation-of-4-sodium-hypochlorite-in-eliminating-enterococcus-faecalis-from-the-root-canal-when-used-with-three-irrigation-methods-an-in-vitro-study
#20
Harsh Priyank, Vinisha Pandey, Abhishek Bagul, Kishore Kumar Majety, Parul Verma, Basanta Kumar Choudhury
INTRODUCTION: Endodontic treatment removes all pathogens, such as Enterococcus faecalis from pulp and root canals. The aim of this study is to assess the usefulness of sodium hypo-chlorite (NaOCl) in removing E. faecalis from the root canal used with three different irrigation methods. MATERIALS AND METHODS: This study was conducted on freshly extracted maxillary incisors. After biomechanical preparation, root canals were injected with E. faecalis. Three groups were made which contained 30 teeth in each group; 2 mL of NaOCl solution was used for irrigation followed by agitation with K-files in group I; 2 mL of NaOCl solution was used for irrigation and ultrasonic agitation was done in group II...
March 1, 2017: Journal of Contemporary Dental Practice
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