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Sterilization and disinfection

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https://www.readbyqxmd.com/read/28077517/two-phase-hospital-associated-outbreak-of-mycobacterium-abscessus-investigation-and-mitigation
#1
Arthur W Baker, Sarah S Lewis, Barbara D Alexander, Luke F Chen, Richard J Wallace, Barbara A Brown-Elliott, Pamela J Isaacs, Lisa C Pickett, Chetan B Patel, Peter K Smith, John M Reynolds, Jill Engel, Cameron R Wolfe, Carmelo A Milano, Jacob N Schroder, Robert D Davis, Matthew G Hartwig, Jason E Stout, Nancy Strittholt, Eileen K Maziarz, Jennifer Horan Saullo, Kevin C Hazen, Richard J Walczak, Ravikiran Vasireddy, Sruthi Vasireddy, Celeste M McKnight, Deverick J Anderson, Daniel J Sexton
BACKGROUND: Nontuberculous mycobacteria (NTM) commonly colonize municipal water and cause healthcare-associated outbreaks. We investigated a biphasic outbreak of Mycobacterium abscessus at a tertiary care hospital. METHODS: Case patients had recent hospital exposure and laboratory-confirmed colonization or infection with M. abscessus from January 2013 through December 2015. We conducted a multidisciplinary epidemiologic, field, and laboratory investigation. RESULTS: The incidence rate of M...
January 11, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/28050984/evaluation-of-bacteriological-profile-in-the-apical-root-segment-of-the-patients-with-primary-apical-periodontitis
#2
Aravind Tatikonda, N Sudheep, Krishna P Biswas, K Gowtham, Sudarshan Pujari, Padam Singh
INTRODUCTION: Apical periodontitis usually results from bacterial accumulation and contamination occurring in the root-canal system, and extending beyond the apical foramen to involve the periapical tissues. Literature has a paucity of the studies that stress on the division and analysis of the pulp canal segments. The reason for this disparity might be the technique used for collecting the samples from the pulp canals. Hence, we carried out the present study to evaluate the microbial flora in the apical part of the roots with necrotic pulp canals...
January 1, 2017: Journal of Contemporary Dental Practice
https://www.readbyqxmd.com/read/28045704/neonatal-peripherally-inserted-central-catheter-practices-and-providers-results-from-the-neonatal-picc1-survey
#3
Elizabeth Sharpe, Latoya Kuhn, David Ratz, Sarah L Krein, Vineet Chopra
BACKGROUND: Neonatal intensive care units (NICUs) commonly utilize peripherally inserted central catheters (PICCs) to provide nutrition and long-term medications to premature and full-term infants. However, little is known about PICC practices in these settings. PURPOSE: To assess PICC practices, policies, and providers in NICUs. METHODS: The Neonatal PICC1 Survey was conducted through the use of the electronic mailing list of a national neonatal professional organization's electronic membership community...
December 29, 2016: Advances in Neonatal Care: Official Journal of the National Association of Neonatal Nurses
https://www.readbyqxmd.com/read/28040502/a-comparison-between-effect-of-photodynamic-therapy-by-led-and-calcium-hydroxide-therapy-for-root-canal-disinfection-against-enterococcus-faecalis-a-randomized-controlled-trial
#4
Mohammad Asnaashari, Hengameh Ashraf, Afsaneh Rahmati, Neda Amini
BACKGROUND: Insufficient root canal disinfection is one of the main reasons for persistent periapical pathology. Photodynamic therapy (PDT) has been proven effective in disinfecting infected root canals. The aim of this study was to evaluate the antimicrobial effect of photo activated disinfection (PAD) when using toluidine blue as photosensitizer and a LED lamp after the conventional treatment, and comparing it with calcium hydroxide therapy in vivo. METHODS: This clinical trial includes 20 patients with molars requiring endodontic retreatment...
December 28, 2016: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/27996305/infection-control-and-patient-discomfort-with-an-alternative-plastic-barrier-in-intraoral-digital-radiography
#5
Arnon Charuakkra, Sangsom Prapayasatok, Apirum Janhom, Karune Verochana, Phattaranant Mahasantipiya
OBJECTIVE: 1) to compare the efficacy of a commercially-available hygienic sheath and an alternative plastic bag in preventing contamination of the imaging plate during intraoral radiography, and 2) to compare patient discomfort when using the hygienic sheath and the plastic bag. METHODS: Sixty sterilized size 2 imaging plates covered with either the hygienic sheath (n = 30) or the plastic bag (n = 30) were used to simulate digital periapical radiographic examination in 30 volunteer patients...
December 20, 2016: Dento Maxillo Facial Radiology
https://www.readbyqxmd.com/read/27989595/analysis-of-genomic-responses-in-a-rat-lung-model-treated-with-a-humidifier-sterilizer-containing-polyhexamethyleneguanidine-phosphate
#6
Min-Seok Kim, Seok Won Jeong, Seong-Jin Choi, Jin-Young Han, Sung-Hwan Kim, Seokjoo Yoon, Jung-Hwa Oh, Kyuhong Lee
The antimicrobial biocide polyhexamethyleneguanidine (PHMG) phosphate is the main ingredient in the commercially available humidifier disinfectant. PHMG phosphate-based humidifier disinfectants can cause pulmonary fibrosis and induce inflammatory and fibrotic responses both in vivo and in vitro. However, toxicological mechanisms including genomic alterations induced by inhalation exposure to PHMG phosphate have not been elucidated. Therefore, this study evaluated the toxicological effects of the PHMG phosphate-containing humidifier disinfectant...
December 15, 2016: Toxicology Letters
https://www.readbyqxmd.com/read/27931630/infectious-complications-in-gastrointestinal-endoscopy-and-their-prevention
#7
REVIEW
Julia Kovaleva
Gastrointestinal endoscopes are medical devices that have been associated with outbreaks of health care-associated infections. Because of the severity and limited treatment options of infections caused by multidrug-resistant Enterobacteriaceae and Pseudomonas aeruginosa, considerable attention has been paid to detection and prevention of these post-endoscopic outbreaks. Endoscope reprocessing involves cleaning, high-level disinfection/sterilization followed by rinsing and drying before storage. Failure of the decontamination process implies the risk of settlement of biofilm producing species in endoscope channels...
October 2016: Best Practice & Research. Clinical Gastroenterology
https://www.readbyqxmd.com/read/27890058/disinfection-and-sterilization-emerging-trends-and-technologies
#8
Marsha Barnden
No abstract text is available yet for this article.
December 2016: AORN Journal
https://www.readbyqxmd.com/read/27882119/bactericidal-effect-of-plasma-jet-with-helium-flowing-through-3-hydrogen-peroxide-against-enterococcus-faecalis
#9
Xin-Cai Zhou, Yu-Lan Li, De-Xi Liu, Ying-Guang Cao, Xin-Pei Lu
The aim of the present study was to assess the antimicrobial activity of plasma jet with helium (He) flowing through 3% hydrogen peroxide in root canals infected with Enterococcus faecalis. A total of 42 single-rooted anterior teeth were prepared, sterilized, inoculated with an E. faecalis suspension and incubated for 7 days. Next, the teeth were randomly divided into six experimental groups (including groups treated by plasma jet with or without He for different time durations) and one control group treated without plasma...
November 2016: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/27878220/diagnosis-of-compliance-of-health-care-product-processing-in-primary-health-care
#10
Camila Eugenia Roseira, Darlyani Mariano da Silva, Isis Pienta Batista Dias Passos, Fabiana Souza Orlandi, Maria Clara Padoveze, Rosely Moralez de Figueiredo
Objective: identify the compliance of health care product processing in Primary Health Care and assess possible differences in the compliance among the services characterized as Primary Health Care Service and Family Health Service. Method: quantitative, observational, descriptive and inferential study with the application of structure, process and outcome indicators of the health care product processing at ten services in an interior city of the State of São Paulo - Brazil...
November 21, 2016: Revista Latino-americana de Enfermagem
https://www.readbyqxmd.com/read/27866488/disposable-bronchoscope-model-for-simulating-endoscopic-reprocessing-and-surveillance-cultures
#11
Mohamed H Yassin, Rahman Hariri, Yasir Hamad, Juliet Ferrelli, Leeanna McKibben, Yohei Doi
BACKGROUND Endoscope-associated infections are reported despite following proper reprocessing methods. Microbiological testing can confirm the adequacy of endoscope reprocessing. Multiple controversies related to the method and interpretation of microbiological testing cultures have arisen that make their routine performance a complex target. OBJECTIVE We conducted a pilot study using disposable bronchoscopes (DBs) to simulate different reprocessing times and soaking times and to compare high-level disinfection versus ethylene oxide sterilization...
November 21, 2016: Infection Control and Hospital Epidemiology
https://www.readbyqxmd.com/read/27866468/intratubular-disinfection-with-tri-antibiotic-and-calcium-hydroxide-pastes
#12
Thais Cristina Pereira, Layla Reginna Silva Munhoz De Vasconcelos, Marcia Sirlene Zardin Graeff, Marco Antonio Hungaro Duarte, Clóvis Monteiro Bramante, Flaviana Bombarda De Andrade
OBJECTIVE: The aim of this study was to compare the in vitro intradentinal antimicrobial ability of the calcium hydroxide and tri-antibiotic pastes. MATERIALS AND METHODS: Standard bovine dentin tubes were sterilized and then infected with Enterococcus faecalis by a new contamination protocol of great depths of dentin. The specimens were filled with the medications, divided into two test-groups: calcium hydroxide (Group 1) and tri-antibiotic (Group 2) pastes. After 15 days, the teeth were evaluated by microbiological culture and confocal laser scanning microscopy (CLSM) with viability dye assay LIVE/DEAD inside dentinal tubules...
November 21, 2016: Acta Odontologica Scandinavica
https://www.readbyqxmd.com/read/27822328/solar-optics-based-active-panel-for-solar-energy-storage-and-disinfection-of-greywater
#13
W Lee, J Song, J H Son, M P Gutierrez, T Kang, D Kim, L P Lee
Smart city and innovative building strategies are becoming increasingly more necessary because advancing a sustainable building system is regarded as a promising solution to overcome the depleting water and energy. However, current sustainable building systems mainly focus on energy saving and miss a holistic integration of water regeneration and energy generation. Here, we present a theoretical study of a solar optics-based active panel (SOAP) that enables both solar energy storage and photothermal disinfection of greywater simultaneously...
September 2016: Biomicrofluidics
https://www.readbyqxmd.com/read/27818222/adenosine-triphosphate-bioluminescence-for-bacteriologic-surveillance-and-reprocessing-strategies-for-minimizing-risk-of-infection-transmission-by-duodenoscopes
#14
Saurabh Sethi, Robert J Huang, Monique T Barakat, Niaz Banaei, Shai Friedland, Subhas Banerjee
BACKGROUND AND AIMS: Recent outbreaks of duodenoscope-transmitted infections underscore the importance of adequate endoscope reprocessing. Adenosine triphosphate (ATP) bioluminescence testing allows rapid evaluation of endoscopes for bacteriologic/biologic residue. In this prospective study we evaluate the utility of ATP in bacteriologic surveillance and the effects of endoscopy staff education and dual cycles of cleaning and high-level disinfection (HLD) on endoscope reprocessing. METHODS: ATP bioluminescence was measured after precleaning, manual cleaning, and HLD on rinsates from suction-biopsy channels of all endoscopes and elevator channels of duodenoscopes/linear echoendoscopes after use...
November 3, 2016: Gastrointestinal Endoscopy
https://www.readbyqxmd.com/read/27805536/-outbreaks-related-to-contaminated-duodenoscopes-causes-and-solutions
#15
A W Rauwers, M C Vos, J W Poley, J G Buijs-Hegeman, M J Bruno
Duodenoscopes for Endoscopic Retrograde Cholangiopancreatography (ERCP) are used for diagnostic and, presently predominantly, for minimally invasive therapeutic procedures involving the biliary tree and the pancreatic duct. In 2012, in the Erasmus MC in the Netherlands, a large outbreak of multidrug-resistant bacteria was caused by a contaminated duodenoscope; its design was such that thorough cleaning was not possible. Worldwide, an increasing number of outbreaks involving multidrug-resistant bacteria caused by contaminated duodenoscopes have been reported on...
2016: Nederlands Tijdschrift Voor Geneeskunde
https://www.readbyqxmd.com/read/27790258/evaluation-of-cytotoxic-effects-of-various-endodontic-irrigation-solutions-on-the-survival-of-stem-cell-of-human-apical-papilla
#16
Narges Farhad Mollashahi, Eshaghali Saberi, Hamed Karkehabadi
INTRODUCTION: Root canal disinfection is an important step in regenerative endodontic treatments. An ideal irrigating solution must have high antimicrobial activity and minimum cytotoxicity. This study sought to assess the effect of some irrigating solutions on stem cells from the human apical papilla (SCAP) after different periods of exposure. METHODS AND MATERIALS: Stem cells were isolated from immature, impacted mandibular third molars, transferred to 24-well plates, randomly divided into 6 experimental groups and exposed to BioPure MTAD Cleanser, QMix, 17% EDTA, 2% chlorhexidine (CHX), 5...
2016: Iranian Endodontic Journal
https://www.readbyqxmd.com/read/27782374/effect-of-disinfection-and-sterilization-on-the-tensile-strength-surface-roughness-and-wettability-of-elastomers
#17
Sunil Babu Kotha, Ravikumar Ramakrishnaiah, Darshan Devang Divakar, Sree Lalita Celur, Saad Qasim, Jukka P Matinlinna
AIM: The aim of the present study was to evaluate the effect of chemical disinfection, autoclave, and microwave sterilization on some of the key properties of elastomers. METHODS: Five polyvinylsiloxane elastomeric impression materials were evaluated. Forty samples were fabricated from each material. The samples were randomly selected and assigned to four experimental groups with 50 samples each: group I, control; group II,chemical disinfection; group III, autoclave sterilization; and group IV, microwave sterilization...
October 26, 2016: Journal of Investigative and Clinical Dentistry
https://www.readbyqxmd.com/read/27778309/infection-prevention-and-ultrasound-probe-decontamination-practices-in-europe-a-survey-of-the-european-society-of-radiology
#18
Christiane Marita Nyhsen, Hilary Humphreys, Carlos Nicolau, Gerhard Mostbeck, Michel Claudon
OBJECTIVES: Although ultrasound (US) is considered one of the safest imaging modalities, concerns have been raised regarding potential infection transmission risks through US procedures. A survey was undertaken by the European Society of Radiology (ESR) to establish infection prevention and control measures in US and to highlight the importance of good medical practice. METHODS: An online survey was sent to all 22,000 full ESR members. RESULTS: The response rate of completed surveys was 4...
December 2016: Insights Into Imaging
https://www.readbyqxmd.com/read/27776820/designing-for-cleanability-the-effects-of-material-surface-roughness-and-the-presence-of-blood-test-soil-and-bacteria-on-devices
#19
Elizabeth A Gonzalez, Poulomi Nandy, Anne D Lucas, Victoria M Hitchins
Cleaning reusable medical devices removes organic and inorganic soil, which allows for effective disinfection and sterilization. However, it is not always clear what variables to consider when validating cleaning. This study compared the ability of 3 different cleaning agents (ie, water, alcohol, and bleach) to remove bacteria (ie, vegetative and spores) and artificial blood test soil from 2 common device materials: polypropylene and ultra-high-molecular-weight polyethylene. There was a complex interaction between bacteria, soil, and surface roughness...
October 21, 2016: American Journal of Infection Control
https://www.readbyqxmd.com/read/27769703/the-practice-of-disinfection-of-high-speed-handpieces-with-70-w-v-alcohol-an-evaluation
#20
Flávia Morais Gomes Pinto, Camila Quartim de Moraes Bruna, Tamara Carolina Camargo, Maíra Marques, Cely Barretos Silva, Suzete M Sasagawa, Lycia Maria Jenné Mimica, Kazuko Uchikawa Graziano
A high-speed handpiece is used in several dental procedures and the official recommendation for safe decontamination consists of rinsing with water, washing with detergent, mechanical friction, and sterilization; however, many professionals only apply 70% w/v alcohol without also cleaning the tool between patients. We performed an analysis of high-speed handpieces reprocessed only with 70% w/v alcohol and found that the methods used in clinical practice are not safe.
October 18, 2016: American Journal of Infection Control
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