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Sterilization and disinfection

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https://www.readbyqxmd.com/read/28727911/ultrasound-processing-of-liquid-system-s-and-its-antimicrobial-mechanism-of-action
#1
David Spiteri, Claire Chot-Plassot, Julie Sclear, Kimon Andreas Karatzas, Chris Scerri, Vasilis Valdramidis
Ultrasound creates cavitation phenomena, resulting in the formation of several free radicals, namely OH• and H•, due to the breakdown of the H2 O molecule. These radicals affect the cellular integrity of the bacteria, causing the inactivation of several processes, and thus it is important to unravel the mechanism of action of this technology. This research looks into the application and mechanism of action of ultrasound technology as a means of disinfection by acoustic cavitation. Sterile water and synthetic waste water were inoculated with different mutants of E...
July 20, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28722122/wet-work-exposure-and-hand-eczema-among-healthcare-workers-a-cross-sectional-study
#2
N Hamnerius, C Svedman, O Bergendorff, J Björk, M Bruze, A Pontén
BACKGROUND: Hand eczema is more common in healthcare workers compared to the general population. The hands are subject to changing occupational exposures due to mandatory hygiene regulations for health care workers. OBJECTIVES: To describe the exposure due to hygiene procedures and investigate the associations between occupational hand washing, use of non-sterile gloves, and hand disinfectant and self-reported hand eczema. METHODS: Cross-sectional study with an electronic questionnaire distributed to 28 762 hospital employees in southern Sweden...
July 19, 2017: British Journal of Dermatology
https://www.readbyqxmd.com/read/28711629/randomized-comparison-of-3-high-level-disinfection-and-sterilization-procedures-for-duodenoscopes
#3
Graham M Snyder, Sharon B Wright, Anne Smithey, Meir Mizrahi, Michelle Sheppard, Elizabeth B Hirsch, Ram Chuttani, Riley Heroux, David S Yassa, Lovisa B Olafsdottir, Roger B Davis, Anastasiou Jiannis, Vijay Bapat, Kiran Bidari, Douglas K Pleskow, Daniel Leffler, Benjamin Lane, Alice Chen, Howard S Gold, Anthony Bartley, Aleah D King, Mandeep S Sawhney
BACKGROUND AND AIMS: Duodenoscopes have been implicated in the transmission of multi-drug resistant bacteria (MDRO). We compared the frequency of duodenoscope contamination with MDRO or any other bacteria after disinfection or sterilization by 3 different methods. METHODS: We performed a single-center prospective randomized study in which duodenoscopes were randomly reprocessed by standard high-level disinfection (sHLD), double high-level disinfection (dHLD), or standard high-level disinfection followed by ethylene oxide gas sterilization (HLD/ETO)...
July 12, 2017: Gastroenterology
https://www.readbyqxmd.com/read/28687186/evaluation-of-a-new-packaging-process-for-non-autoclavable-endoscopes-results-for-the-first-100-microbiological-samples
#4
C Minebois, P Saviuc, J Shum, P Tuvignon, I Pelloux, M-P Brenier-Pinchart, C Landelle, M-R Mallaret
BACKGROUND: A new process for packaging endoscopes (SureStore(®), Medical Innovations Group) immediately after they exit from washing and disinfection in an automated endoscope reprocessor (AER) allows for endoscopes to be stored for up to 15 days. AIM: To describe the microbiological quality of samples from gastrointestinal endoscopes following this process. METHODS: Three-month prospective study using microbiological sampling from a stock of 38 gastrointestinal endoscopes carried out in a French University Hospital...
July 4, 2017: Journal of Hospital Infection
https://www.readbyqxmd.com/read/28681083/a-novel-protocol-obviates-endoscope-sampling-for-carbapenem-resistant-enterobacteriaceae-experience-of-a-center-with-a-prior-outbreak
#5
Zachary L Smith, Arshish Dua, Kia Saeian, Nathan A Ledeboer, Mary Beth Graham, Murad Aburajab, Darren D Ballard, Abdul H Khan, Kulwinder S Dua
BACKGROUND: Numerous published outbreaks, including one from our institution, have described endoscope-associated transmission of multidrug-resistant organisms (MDROs). Individual centers have adopted their own protocols to address this issue, including endoscope culture and sequestration. Endoscope culturing has drawbacks and may allow residual bacteria, including MDROs, to go undetected after high-level disinfection. AIM: To report the outcome of our novel protocol, which does not utilize endoscope culturing, to address our outbreak...
July 5, 2017: Digestive Diseases and Sciences
https://www.readbyqxmd.com/read/28677080/comparison-of-impact-of-two-decontamination-solutions-on-the-viability-of-the-cells-in-human-amnion
#6
Ingrida Smeringaiova, Peter Trosan, Miluse Berka Mrstinova, Jan Matecha, Jan Burkert, Jan Bednar, Katerina Jirsova
Human amniotic membrane (HAM) is used as an allograft in regenerative medicine or as a source of pluripotent cells for stem cell research. Various decontamination protocols and solutions are used to sterilize HAM before its application, but little is known about the toxicity of disinfectants on HAM cells. In this study, we tested two decontamination solutions, commercial (BASE·128) and laboratory decontamination solution (LDS), with an analogous content of antimycotic/antibiotics for their cytotoxic effect on HAM epithelial (EC) and mesenchymal stromal cells (MSC)...
July 4, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28676670/species-specific-antifungal-activity-of-blue-light
#7
Wioleta J Trzaska, Helen E Wrigley, Joanne E Thwaite, Robin C May
Fungal pathogens represent a significant threat to immunocompromised patients or individuals with traumatic injury. Strategies to efficiently remove fungal spores from hospital surfaces and, ideally, patient skin thus offer the prospect of dramatically reducing infections in at-risk patients. Photodynamic inactivation of microbial cells using light holds considerable potential as a non-invasive, minimally destructive disinfection strategy. Recent data indicate that high-intensity blue light effectively removes bacteria from surfaces, but its efficacy against fungi has not been fully tested...
July 4, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28669067/determining-the-optimal-dose-of-1940-nm-thulium-fiber-laser-for-assisting-the-endodontic-treatment
#8
Ayse Sena Kabas Sarp, Murat Gulsoy
Insufficient cleaning, the complex anatomy of the root canal system, inaccessible accessory canals, and inadequate penetration of irrigants through dentinal tubules minimizes the success of the conventional endodontic treatment. Laser-assisted endodontic treatment enhances the quality of conventional treatment, but each laser wavelength has its own its own limitations. The optimal parameters for the antibacterial efficiency of a new wavelength, 1940-nm Thulium Fiber Laser, were firstly investigated in this study...
July 1, 2017: Lasers in Medical Science
https://www.readbyqxmd.com/read/28665993/knowledge-attitudes-and-practice-on-the-prevention-of-central-line-associated-bloodstream-infections-among-nurses-in-oncological-care-a-cross-sectional-study-in-an-area-of-southern-italy
#9
Maria Rosaria Esposito, Assunta Guillari, Italo Francesco Angelillo
The objectives of the cross-sectional study were to delineate the knowledge, attitudes, and behavior among nurses regarding the prevention of central line-associated bloodstream infections (CLABSIs) and to identify their predisposing factors. A questionnaire was self-administered from September to November 2011 to nurses in oncology and outpatient chemotherapy units in 16 teaching and non-teaching public and private hospitals in the Campania region (Italy). The questionnaire gathered information on demographic and occupational characteristics; knowledge about evidence-based practices for the prevention of CLABSIs; attitudes towards guidelines, the risk of transmitting infections, and hand-washing when using central venous catheter (CVC); practices about catheter site care; and sources of information...
2017: PloS One
https://www.readbyqxmd.com/read/28662202/innovative-non-thermal-plasma-disinfection-process-inside-sealed-bags-assessment-of-bactericidal-and-sporicidal-effectiveness-in-regard-to-current-sterilization-norms
#10
Zouhaier Ben Belgacem, Gaëlle Carré, Emilie Charpentier, Florian Le-Bras, Thomas Maho, Eric Robert, Jean-Michel Pouvesle, Franck Polidor, Sophie C Gangloff, Mohamed Boudifa, Marie-Paule Gelle
In this work, we developed a device capable to generate a non-thermal plasma discharge inside a sealed bag. The aim of this study was to assess the effectiveness of the oxygen, nitrogen and argon plasma sterilization on Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis spores according to the NF EN 556 Norm. Moreover the bag integrity which is a critical key to maintain the sterile state of items after the end of the process was verified by Fourier Transform Infrared (FTIR) and X-ray Photoelectron Spectrometry (XPS) analyses...
2017: PloS One
https://www.readbyqxmd.com/read/28655955/comparison-of-efficacy-and-cost-effectiveness-of-0-55-ortho-phthalaldehyde-and-2-glutaraldehyde-for-disinfection-of-laryngoscopes-a-prospective-pilot-study
#11
Priyanka Pradeep Karnik, Nandini Malay Dave, Gita Nataraj, Rajarshi Gupta, Madhu Garasia
BACKGROUND AND AIMS: The laryngoscope is a potential source of cross-infection as it involves contact with the mucous membrane, saliva and occasionally blood. This study compared efficacy and cost-effectiveness of two Centre for Disease Control approved agents for disinfection of laryngoscope blades. METHODS: One hundred and sixty patients requiring laryngoscopy and intubation for general anaesthesia were randomly allocated into two groups. After tracheal intubation, used laryngoscope blades were cleaned with tap water...
June 2017: Indian Journal of Anaesthesia
https://www.readbyqxmd.com/read/28650137/how-to-manage-the-biological-risk-in-a-dental-clinic-state-of-the-art-and-future-perspectives
#12
Matteo Saccucci, Gaetano Ierardo, Carmela Protano, Matteo Vitali, Antonella Polimeni
Dental Personnel (DP) may be exposed to pathogens during dental treatment, either through contact contaminated equipment, or with blood and respiratory secretion. On the other hand, health care professionals are constantly exposed to pathogens and opportunists in their work environment. Consequently, the dental healthcare environment is connected with the risk of exposure to biological agents both for patients and dental workers, and involves a wide number of microorganisms that can be present in biological matrices (gingival fluids, saliva, blood), contaminated and/or non-sanitized surfaces, water used in the dental unit, or emitted by patients suffering or carrier of a transmissible disease...
June 23, 2017: Minerva Stomatologica
https://www.readbyqxmd.com/read/28638017/-intraoperative-disinfection-sterilization-and-repeated-use-of-infected-titanium-implant-in-treatment-of-scalp-radial-ulcer-with-recurrent-meningioma
#13
V I Sharobaro, A G Gavrilov, N E Manturova
No abstract text is available yet for this article.
2017: Khirurgiia
https://www.readbyqxmd.com/read/28621298/is-the-stella%C3%A2-5l-system-an-effective-cold-sterilization-technique-for-needle-based-confocal-miniprobes
#14
Cai-Xia Wang, Yuan-Yuan Chen, Feng Yang, Fan Yang, Jin-Tao Guo, Siyu Sun, Ling Fan
BACKGROUND AND OBJECTIVE: Needle-based confocal laser endomicroscopy (nCLE) under endoscopic ultrasound guidance is a newly developed imaging technique for pancreatic lesions and lymph nodes, enabling a subcellular level of resolution. The confocal miniprobe is an invasive instrument designed to be reused up to 10 times. Therefore, a method that ensures the complete elimination of microbial contaminants on the device is necessary. We studied the bactericidal efficacy of the Stella™ system, which purports to achieve this objective...
May 2017: Endoscopic Ultrasound
https://www.readbyqxmd.com/read/28605891/bacterial-contamination-and-disinfection-status-of-laryngoscopes-stored-in-emergency-crash-carts
#15
Jae Hyung Choi, Young Soon Cho, Jung Won Lee, Hee Bong Shin, In Kyung Lee
Objectives: To identify bacterial contamination rates of laryngoscope blades and handles stored in emergency crash carts by hospital and area according to the frequency of intubation attempts. Methods: One hundred forty-eight handles and 71 blades deemed ready for patient use from two tertiary hospitals were sampled with sterile swabs using a standardized rolling technique. Samples were considered negative (not contaminated) if no colonies were present on the blood agar plate after an 18-hour incubation period...
2017: Journal of Preventive Medicine and Public Health, Yebang Ŭihakhoe Chi
https://www.readbyqxmd.com/read/28584537/a-comparison-of-the-efficacy-of-mechanical-chemical-and-microwave-radiation-methods-in-disinfecting-complete-dentures
#16
Niloofar Mojarad, Zahra Khalili, Shima Aalaei
BACKGROUND: Poor denture hygiene can be a potential source of pathogens. The aim of this study was to compare the efficacy of microwave radiation with that of chemical and mechanical techniques in disinfecting complete dentures contaminated with Staphylococcus aureus and Pseudomonas aeruginosa. MATERIALS AND METHODS: Seventy-two sterilized mandibular dentures were separately contaminated with S. aureus (n = 32) and P. aeruginosa (n = 32) and then incubated at 37°C for 48 h...
March 2017: Dental Research Journal
https://www.readbyqxmd.com/read/28557780/microbiologic-testing-for-503a-sterile-compounding-pharmacies
#17
William Mixon, Abby Roth
Compounding pharmacists must ensure that the sterile preparations they dispense are free of microbiologic contamination. Working in a cleanroom under controlled conditions (proper differential air pressure, temperature, and humidity; acceptable levels of viable and nonviable airborne particles and surface counts, etc.) and testing the efficacy of cleaning and disinfecting practices via environmental monitoring (viable-air and surface testing, glove-fingertip-thumb testing, etc.) are essential to preparing contamination-free medications...
May 2017: International Journal of Pharmaceutical Compounding
https://www.readbyqxmd.com/read/28557536/preoperative-hand-decontamination-in-ophthalmic-surgery-a-comparison-of-the-removal-of-bacteria-from-surgeons-hands-by-routine-antimicrobial-scrub-versus-an-alcoholic-hand-rub
#18
Yaara Forer, Colin Block, Shahar Frenkel
PURPOSE: The goal of this experiment was to evaluate and compare the antimicrobial efficacy of routine preoperative hand washing using commercial medicated sponge brushes versus an alcoholic hand rub, by comparing bacterial growth on ophthalmic surgeons' hands after application of each of these methods. METHODS: Twenty ophthalmic surgeons were recruited at the Hadassah-Hebrew University Medical Center in Jerusalem, Israel. Samples were collected twice from the hands of each surgeon after hand decontamination using two different protocols during routine surgical practice...
May 30, 2017: Current Eye Research
https://www.readbyqxmd.com/read/28549510/understanding-the-current-state-of-infection-preventionists-through-competency-role-and-activity-self-assessment
#19
Ericka L Kalp, James F Marx, James Davis
BACKGROUND: The Association for Professionals in Infection Control and Epidemiology (APIC) MegaSurvey, administered in 2015, was completed by approximately 4,079 APIC members. The survey sought to gain a better understanding the current state of 4 components of infection prevention practice: demographic characteristics, compensation, organizational structure, and practice and competency. METHODS: The data for this analysis come from the APIC MegaSurvey Practice and Competency domain...
June 1, 2017: American Journal of Infection Control
https://www.readbyqxmd.com/read/28540102/awareness-and-knowledge-of-undergraduate-dental-students-about-sterilization-disinfection-methods-of-extracted-human-teeth
#20
S C Deogade, S S Mantri, S Saxena, K Sumathi
BACKGROUND: Dental undergraduate students work on extracted human teeth in preclinical practical's to learn technical skills before entering the clinics and delivering dental care to the patients. AIM: The aim of the present investigation was to assess the awareness and knowledge toward sterilization/disinfection methods of extracted human teeth in a selected group of Indian dental students. MATERIALS AND METHODS: In this descriptive cross-sectional study, the participants consisted of 2(nd)-, 3(rd)-, 4(th)-, and 5(th)-year dental students...
November 2016: Annals of Medical and Health Sciences Research
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