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Sterilization and disinfection

Letícia Kraft, Victoria Stadler Tasca Ribeiro, Luciana Cristina Wollmann, Paula Hansen Suss, Felipe Francisco Tuon
All cardiac allograft tissues are under potential contamination, requiring a validated terminal sterilization process or a minimal bioburden. The bioburden calculation is important to determine the bacterial burden and further decontamination and disinfection strategies for the valve processing. The aim of this study was to determine the bioburden from transport solution (TS) of heart valves obtained from non-heart-beating and heart-beating donors in different culture methods. The bioburden from TS was determined in 20 hearts donated for valve allograft tissue using membrane filter (MF) and direct inoculation...
March 19, 2018: Cell and Tissue Banking
Ojan Assadian, Paul N Humphreys, Karen J Ousey
Disinfection of gloved hands is increasingly advocated in situations where visibly not soiled gloves are used during multiple clinical activities on the same patient. Since there is no data demonstrating that such practice attributes to lower bacterial transfer during clinical care, a standardised experimental study was conducted. Gloved hands touched chicken breasts contaminated with Staphylococcus epidermidis with or without disinfection before toughing sterile catheter valves. Contaminated gloves transferred 5...
March 15, 2018: Journal of Hospital Infection
Chen Bin Tian
OBJECTIVE: The aim of this study was to explore the effects of acupuncture on mild postpartum depression (PPD). METHODS: 15 patients diagnosed with PPD were enrolled in the study. Patients who satisfied the inclusion criteria received acupuncture therapy eight times over 4 weeks in the form of two 20-min treatment sessions per week. The treatment site was the BL67 acupuncture point (on the outside of the fifth toenail). After disinfecting the skin on the toes, sterile steel needles were gently inserted into the acupuncture point until the patient started to feel numb or swollen, a sensation known as de qi ...
March 14, 2018: Acupuncture in Medicine: Journal of the British Medical Acupuncture Society
P Montero-Miralles, J Martín-González, O Alonso-Ezpeleta, M C Jiménez-Sánchez, E Velasco-Ortega, J J Segura-Egea
Regenerative endodontic procedures (REPs) are biologically based procedures planned to replace damaged tissues, including dentinee and root structures, as well as cells of the pulp-dentine complex. Effective sterilization of the root canal is essential in REPs, and antibiotics have been widely used to disinfect root canals. The aim of this paper was to review the scientific literature on 1) Effectiveness of antibiotics used in REPs against bacteria implicated in endodontic disease; 2) Scientific evidence supporting the use of topical antibiotics in REPs; 3) Clinical implications of the use of antibiotics in REPs and the possible side effects; 4) Effect of antibiotics on dental pulp stem cells; and 5) Ongoing research on the use of antibiotics in REPs...
February 26, 2018: International Endodontic Journal
Xia Su, Ying Tian, Hongzhuan Zhou, Yinglong Li, Zhenhua Zhang, Beiyu Jiang, Bing Yang, Jue Zhang, Jing Fang
In recent years, plasma activated solution (PAS) have made a good progress in the disinfection of medical device, tooth whitening, fruit preservation. In this study, we investigated the inactivation efficacy of Newcastle disease virus by PAS. Water, 0.9% NaCl and 0.3% H2 O2 were excited by plasma to obtain the corresponding solutions PAS(H2 O), PAS(NaCl) and PAS(H2 O2 ). The complete inactivation of virus after PAS treatment for 30 min was confirmed by the embryo lethality assay (ELA) and hemagglutination (HA) test...
February 23, 2018: Applied and Environmental Microbiology
Moi Lin Ling, Patricia Ching, Ammar Widitaputra, Alison Stewart, Nanthipha Sirijindadirat, Le Thi Anh Thu
Background: The Asia Pacific Society of Infection Control launched its revised Guidelines for Disinfection and Sterilization of Instruments in Health Care Facilities in February 2017. This document describes the guidelines and recommendations for the reprocessing of instruments in healthcare setting. It aims to highlight practical recommendations in a concise format designed to assist healthcare facilities at Asia Pacific region in achieving high standards in sterilization and disinfection...
2018: Antimicrobial Resistance and Infection Control
Sang Hye Ji, Se Hoon Ki, Ji Ho Ahn, Jae Ho Shin, Eun Jeong Hong, Yun Ji Kim, Eun Ha Choi
This study focused on sterilization methods for the reduction of microorganisms on perilla leaves by cylinder type Dielectric Barrier Discharge (DBD) plasma with underwater bubbler treatment. S. aureus and E. coli in a suspension were reduced to less than 3.4 and 0.5 log CFU/ml after the plasma treatment for 3 min, respectively. On the perilla leaves, they were also reduced to 4.8 and 1.6 log CFU/ml after the plasma treatment, respectively. The S. aureus and E. coli bacterial cell wall was damaged by the plasma treatment evident by scanning electron microscopic analysis...
February 15, 2018: Archives of Biochemistry and Biophysics
Nicole Atkins, William Hodge, Bruce Li
Tonometry has been identified as a common method for measuring the intraocular pressure in patients. The direct contact between the tonometer and the eye may contribute to the risk of cross infection, especially of viral particles, from one patient to another. A systematic review was undertaken to address the likelihood of human immunodeficiency virus (HIV), hepatitis B virus, hepatitis C virus, and prion diseases transmission through the use of tonometers. Additionally, a comparison of the current tonometer disinfection methods is provided to assist with identifying which technique effectively reduces the risk of disease transmission...
March 2018: Journal of Clinical Medicine Research
Tengyi Zheng, Xintian Huang, Jintian Chen, Disang Feng, Liling Mei, Ying Huang, Guilan Quan, Chune Zhu, Vikramjeet Singh, Hao Ran, Xin Pan, Chuan-Yu Wu, Chuanbin Wu
Lyotropic liquid crystals (LLC) have received increasing attention as a drug delivery system. In this study, a novel intra-canal disinfectant based on the glycerol monooleate (GMO) LLC precursor incorporation with chlorhexidine (CHX) and silver nanoparticles (Ag-NPs) was designed and evaluated. The LLC precursor with excellent fluidity was able to penetrate deeply into the complex tiny collateral branch root canals. The transformation of cubic LLC in root canals upon coming into contact with water provided long-lasting disinfection against multidrug-resistant bacteria to avoid the endodontic reinfection and follow-up visits...
February 6, 2018: Biomaterials Science
Ali Asghar Ayatollahi, Abolfazl Amini, Somayeh Rahimi, Saeid Rahbar Takrami, Reza Kazemi Darsanaki, Muhammad Sadeqi Nezhad
Nosocomial infections are one of the most common causes of death in hospitals. This study aimed to determine the prevalence of gram-negative bacilli isolated from the equipment in hospital wards of the Golestan province, in the year 2015. In this cross-sectional study in 2015, 1980 samples from medical and nonmedical equipment and surfaces were collected from the wards of 13 teaching hospitals, in the Golestan province. Samples were inoculated into eosin methylene blue agar and blood agar culture media and isolated colonies were identified by standard biochemical tests...
December 18, 2017: European Journal of Microbiology & Immunology
John M Boyce
Isopropyl alcohol and ethyl alcohol have been used as low-level disinfectants in healthcare settings for many years. Recent studies have found that ethyl alcohol inhibits protein synthesis in Escherichia coli by direct effects on ribosomes and RNA polymerase and that 60%-70% solutions have in vitro efficacy against murine norovirus, Ebola virus, and several coronaviruses. Alcohol prep pads or towelettes containing isopropyl or ethyl alcohol and water have been used primarily for disinfection of small noncritical items due to a concern regarding their rapid evaporation rates and associated short contact times...
January 28, 2018: Infection Control and Hospital Epidemiology
Farhad Hajizadeh, Bahman Derakhshan, Ali Peimani, Zahra Abbasi
AIM: In medicine, honey is known for its various biological or pharmacological effects, from wound dressing to anticancero-genic and from anti-inflammatory to antibacterial activities. The aim of the current study was to evaluate the effect of honey on healing of mandibular bone defects in a rat model. MATERIALS AND METHODS: This animal study was performed on 24 wild-type Wistar rats. Following shaving, disinfection, and extraoral incision, a 2 × 2 mm defect was created at mandibular angle...
January 1, 2018: Journal of Contemporary Dental Practice
Maryam Pourhajibagher, Reza Raoofian, Roghayeh Ghorbanzadeh, Abbas Bahador
BACKGROUND: The infected root canal system harbors one of the highest accumulations of polymicrobial infections. Since the eradication of endopathogenic microbiota is a major goal in endodontic infection therapy, photo-activated disinfection (PAD) can be used as an alternative therapeutic method in endodontic treatment. Compared to cultivation-based approaches, molecular techniques are more reliable for identifying microbial agents associated with endodontic infections. The purpose of this study was to evaluate the ability of designed multiplex real-time PCR protocol for the rapid detection and quantification of six common microorganisms involved in endodontic infection before and after the PAD...
January 11, 2018: Photodiagnosis and Photodynamic Therapy
Namith Rai, Rajaram Naik, Ravi Gupta, Shobana Shetty, Amith Singh
Aim of the Study: This study aims to evaluate the effects three different conditioning agents on the shear bond strength of resin-modified glass ionomers to human dentin. Materials and Methods: One hundred and twenty recently extracted, caries-free premolars and molars will be cleaned of debris and disinfected in a 0.5% solution of sodium hypochlorite and sterile water for 30 min. The occlusal surface of each tooth will be reduced using conventional model trimmer with water to produce the dentin surface...
October 2017: Contemporary Clinical Dentistry
Carlos A Gómez-Aldapa, Esmeralda Rangel-Vargas, Ma Refugio Torres-Vitela, Angélica Villarruel-López, Otilio A Acevedo-Sandoval, Alberto J Gordillo-Martínez, Angélica Godínez-Oviedo, Javier Castro-Rosas
Leafy greens have been associated with foodborne disease outbreaks in different countries. To decrease microbial contamination of leafy greens, chemical agents are commonly used; however, a number of studies have shown these agents to have limited antimicrobial effect against pathogenic bacteria on vegetables. The objective of this study was to compare the antibacterial effect of Hibiscus sabdariffa calyx extracts (water, methanol, acetone, and ethyl acetate), sodium hypochlorite, acetic acid, and colloidal silver against foodborne bacteria on leafy greens...
January 10, 2018: Journal of Food Protection
Fatma Neslihan Yuksel, Neslihan Taskale Karatug, Mustafa Akcelik
Biofilm structures are the most resistant form of active microorganisms against sanitation, disinfection, and sterilization processes. One of the specific properties of biofilm is the development of antibiotic resistance that can be up to 1,000-fold greater than planktonic cells. Enterococcus faecium is a human pathogen that causes nosocomial bacteremia and at the present time, it is well known that most of the chronic infections are biofilm-based. Recent evidence suggested that subinhibitory concentrations (sub-MICs) of antibiotics have an important role in the evolution of antibiotic resistance and induction on biofilm formation...
December 15, 2017: Acta Microbiologica et Immunologica Hungarica
Francisco Martins, Patrícia Branco, José Reis, Ignacio Barbero Navarro, Paulo Maurício
Objective: Oral rehabilitation success is enhanced by an accurate and reproducible final impression. The purpose of this study is to evaluate the dimensional changes of a polyether and addition silicone subjected to disinfection and/or sterilization after a long storage period. Material and methods: Ninety samples were obtained from polyether ImpregumTM PentaTM (3M ESPETM, Seefeld, Germany) and 90 of addition silicone ImprintTM 4 PentaTM Putty (3M ESPETM, Seefeld, Germany) according to ISO 4823:2000. The samples of each material were split to form three groups with 30 samples each: a control group, a hypochlorite group (disinfection) and an autoclave group (sterilization)...
2017: Acta Biomaterialia Odontologica Scandinavica
Stephanie Loeb, Chuanhao Li, Jae-Hong Kim
A simple heat treatment, perhaps the most globally recognized point-of-use water sterilization method, is seemingly effective against all major pathogens of concern, but bulk water boiling is not energy efficient or sustainable. Herein, we present the first application of solar-to-thermal converting nanomaterials for the direct inactivation of bacteria and viruses in drinking water through the application of Au nanorods, carbon black, and Au nanorod-carbon black composite materials as light absorbers. With broad absorption bands spanning the visible and near-infrared wavelengths, at sufficient concentrations, these nanoparticles induce multiple scattering events, increasing photon absorption probability and concentrating the light within a small spatial domain, leading to localized, intense heating that inactivates microorganisms in close proximity...
December 14, 2017: Environmental Science & Technology
Xin Fan, Fan Yang, Chuanxiong Nie, Ye Yang, Haifeng Ji, Chao He, Chong Cheng, Changsheng Zhao
Pathogenic bacterial infection has been becoming a global threat toward people's health, especially the massive usage of antibiotics due to the lack of antibacterial agents with less side effects. Developing new nanoagents to fight pathogenic bacteria has provided enormous new possibilities in the treatment of bacterial infections, such as graphene-based two-dimensional (2D) antibacterial nanoagents with different bacterial inhibition capabilities; however, mussel-inspired design of near-infrared (NIR)-responsive and biocompatible Ag-graphene nanoagents possessing efficient and versatile bacterial disinfection activities have rarely been reported...
January 10, 2018: ACS Applied Materials & Interfaces
Marilyn C Erickson, Jye-Yin Liao, Cathy C Webb, Mussie Y Habteselassie, Jennifer L Cannon
Gloves are worn by workers harvesting ready-to-eat produce as a deterrent for contaminating the produce with enteric pathogens that may reside on their hands. As fields are not sterile environments, the probability for gloves to become contaminated still exists and therefore it is critical to understand the conditions that affect the survival of pathogens on gloves. Both Escherichia coli O157:H7 and Salmonella deposited on glove surfaces in a liquid state survived longer when the pathogen had been suspended in lettuce sap than when suspended in water...
February 2, 2018: International Journal of Food Microbiology
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