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Sterilization and disinfection

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https://www.readbyqxmd.com/read/28520859/the-role-of-patient-care-items-as-a-fomite-in-healthcare-associated-outbreaks-and-infection-prevention
#1
Hajime Kanamori, William A Rutala, David J Weber
Patient-care items can serve as a source or reservoir for healthcare associated pathogens in hospitals. We reviewed healthcare-associated outbreaks from medical equipment and provided infection prevention recommendations. Multiple healthcare-associated outbreaks via a contaminated patient-care item were identified, including infections with multidrug-resistant organisms. The type of patient-care items implicated as a fomite causing healthcare-associated infections (HAI) has changed over time. Patient populations at risk were most commonly critically ill patients in adult and neonatal ICUs...
May 17, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/28518179/cleaning-disinfection-and-sterilization-protocols-employed-for-customized-implant-abutments-an-international-survey-of-100-universities-worldwide
#2
Luigi Canullo, Marco Tallarico, Stephen Chu, David Pennarocha, Mutlu Özcan, Paolo Pesce
PURPOSE: American and European standards recommend sterilization of customized abutments before connecting them to implants, as customized abutments are considered semi-critical medical devices. Since standardized procedures could not be identified in the literature on implantology, this survey evaluated the protocols employed at different universities worldwide to clean, disinfect, and/or sterilize customized abutments before their connection to bone-level implants. MATERIALS AND METHODS: The survey took place between October 2015 and January 2016...
May 18, 2017: International Journal of Oral & Maxillofacial Implants
https://www.readbyqxmd.com/read/28516730/control-of-pseudomonas-mastitis-on-a-large-dairy-farm-by-using-slightly-acidic-electrolyzed-water
#3
Kazuhiro Kawai, Yasunori Shinozuka, Ikuo Uchida, Kazuhiko Hirose, Takashi Mitamura, Aiko Watanabe, Kana Kuruhara, Reiko Yuasa, Reiichiro Sato, Ken Onda, Hajime Nagahata
The disinfection effect of slightly acidic electrolyzed water (SAEW) use in a farm where Pseudomonas mastitis has spread was evaluated. Despite the application of antibiotic therapy and complete cessation of milking infected quarters, numerous new and recurrent Pseudomonas aeruginosa clinical mastitis infections (5.8-7.1% of clinical mastitis cases) occurred on the farm from 2003 to 2005. Procedural changes and equipment modifications did not improve environmental contamination or the incidence of Pseudomonas mastitis...
May 18, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28511502/comparative-evaluation-of-three-herbal-solutions-on-the-disinfection-of-guttapercha-cones-an-in-vitro-study
#4
Pratap Kumar Mukka, Samba Shiva Rao Pola, Nanda Kumar Kommineni, Mayuri Sailaja Pachalla, Anand Ramtheerth Karne, Karthik Labishetty, Supriya Kommineni, Praveen Chilakabathini
INTRODUCTION: Guttapercha (GP) cones are usually supplied in aseptic, sealed packages from the manufacturers, but once exposed to the dental office environment or even by handling; they can be contaminated by a number of microorganisms. Supplementary decontamination of GP cones is critical. As they cannot be sterilized by moist or dry heat, cold sterilization, using disinfectants should be used. AIM: The aim of the study was to evaluate rapid, reliable, convenient and effective method of disinfection of GP cones using readily available herbal solutions such as Aloevera Juice, Amla Juice and Pancha Tulsi...
March 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28479760/reprocessing-and-reuse-of-urological-armamentarium-how-correct-are-we
#5
REVIEW
Krutik Vipulbhai Raval, Rajeev Chaudhari, Shahil Rameshbhai Khant, Omkar Joglekar, Dipen Patel
Healthcare is expensive for a large proportion of the population in spite of high per capita income and good health insurance penetration. In an effort to reduce cost of the procedure, reprocessing of devices was started in the late 1970s. Reprocessing practice includes various measures such as proper cleaning, disinfection, and sterilization procedures. As reprocessing is aimed at reducing cost, there is a potential risk of compromising patient safety due to cross contamination after inadequate sterilization...
April 2017: Urology Annals
https://www.readbyqxmd.com/read/28470936/supercritical-co2-assisted-process-for-the-production-of-high-purity-and-sterile-nano-hydroxyapatite-chitosan-hybrid-scaffolds
#6
G Ruphuy, M Souto-Lopes, D Paiva, P Costa, A E Rodrigues, F J Monteiro, C L Salgado, M H Fernandes, J C Lopes, M M Dias, M F Barreiro
Hybrid scaffolds composed of hydroxyapatite (HAp), in particular in its nanometric form (n-HAp), and chitosan (CS) are promising materials for non-load-bearing bone graft applications. The main constraints of their production concern the successful implementation of the final purification/neutralization and sterilization steps. Often, the used purification strategies can compromise scaffold structural features, and conventional sterilization techniques can result in material's thermal degradation and/or contamination with toxic residues...
May 4, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28440891/evaluation-of-three-different-decontamination-techniques-on-biofilm-formation-and-on-physical-and-chemical-properties-of-resin-composites
#7
Carolina Bosso André, Andressa Dos Santos, Carmem Silvia Pfeifer, Marcelo Giannini, Emerson Marcelo Girotto, Jack Liborio Ferracane
OBJECTIVES: This study evaluated three different sterilization/disinfection techniques for resin composites on bacterial growth and surface modification after decontamination. METHODS: Two resin composites were sterilized/disinfected with three different techniques: UV light, 1% chloramine T, and 70% ethanol. Four different times were used for each technique to determine the shortest time that the solution or UV light was effective. The influence of sterilization/disinfection technique on bacterial growth was evaluated by analyzing the metabolic activity, using the AlamarBlue™ assay, bacterial viability, and SEM images from biofilms of Streptococcus mutans...
April 25, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28434755/-evaluation-of-the-operating-results-and-costs-associated-with-the-implementation-of-a-flexible-ureteroscopy-activity-within-a-university-hospital-center
#8
K Del Santo, M Audouin, I Ouzaid, V Ravery, O Traxer
INTRODUCTION: The increasing use of flexible ureteroscopy (USSR) sets the cost problems inevitably associated with the fragility of these endoscopes. The aim of this work is to clear from a recent example (universitary hospital) results, costs during the implementation of this technique. MATERIAL AND METHODS: This is a retrospective study conducted from December 2012 (date of introduction of the activity) in March 2015 concerning the USSR made in a French universitary hospital for stone disease...
May 2017: Progrès en Urologie
https://www.readbyqxmd.com/read/28431851/ultraviolet-c-light-as-a-means-of-disinfecting-anesthesia-workstations
#9
Matthew Nottingham, Gene Peterson, Christopher Doern, Michelle Doll, Nadia Masroor, Kakotan Sanogo, Michael Stevens, Gonzalo Bearman
BACKGROUND: Anesthesia workstations (AWs) are a reservoir for pathogenic organisms potentially associated with surgical site infections. This study examined the effectiveness of the Tru-D SmartUVC device (Tru-D LLC, Nashville, TN) on bioburden reduction (BR) on AWs. METHODS: Strips of tissue inoculated with a known concentration of either Staphylococcus aureus, Enterococcus faecalis, or Acinetobacter sp were placed on 22 high-touch surfaces of an AW. Half of the AW surfaces received direct ultraviolet (UV) light exposure and half received indirect exposure...
April 18, 2017: American Journal of Infection Control
https://www.readbyqxmd.com/read/28426292/efficacy-of-three-different-lasers-on-eradication-of-enterococcus-faecalis-and-candida-albicans-biofilms-in-root-canal-system
#10
Silvija Kasić, Marita Knezović, Nataša Beader, Dragana Gabrić, Ana Ivanišević Malčić, Anja Baraba
OBJECTIVE: The objective was to compare the efficacy of three different lasers in disinfection of root canals inoculated with Enterococcus faecalis and Candida albicans biofilms. BACKGROUND: Endodontic space disinfection depends on the type of root canal irrigant used and the way it is delivered and agitated because irrigants have limited ability to reach all parts of root canal system. MATERIALS AND METHODS: Thirty single-rooted human teeth were selected...
April 20, 2017: Photomedicine and Laser Surgery
https://www.readbyqxmd.com/read/28422353/comprehensive-review-and-comparison-of-the-disinfection-techniques-currently-available-in-the-literature
#11
REVIEW
Ahila S Chidambaranathan, Muthukumar Balasubramanium
The goal of disinfection in the dental office is to prevent the spread of infection from one patient to another and maintain the safety of the dental care providers. Prevention of cross infection has significant effect on infection control. The standard procedure of rinsing impressions under tap water immediately after removal from the mouth eliminates microorganisms along with saliva and blood. A broad search on published literature was done using the keywords impression materials, disinfection method, and sterilization of dental impression from 1980 to 2016 in Medline, Google Scholar, the internet, and textbooks...
April 19, 2017: Journal of Prosthodontics: Official Journal of the American College of Prosthodontists
https://www.readbyqxmd.com/read/28396277/duodenoscope-reprocessing-surveillance-with-adenosine-triphosphate-testing-and-terminal-cultures-a-clinical-pilot-study
#12
Kavel Visrodia, Yuri Hanada, Kelly M Pennington, Pritish K Tosh, Mark D Topazian, Bret T Petersen
BACKGROUND & AIMS: Recent reports of infectious outbreaks linked to duodenoscopes have led to proposals for duodenoscope surveillance culturing, which has inherent limitations. We aimed to assess the feasibility of real-time adenosine triphosphate (ATP) testing after manual cleaning, and its ability to predict reprocessing adequacy, as determined by terminal duodenoscope cultures. METHODS: Clinically used duodenoscopes underwent reprocessing per current guidelines...
April 7, 2017: Gastrointestinal Endoscopy
https://www.readbyqxmd.com/read/28379193/a-force-visualized-silicone-retractor-attachable-to-surgical-suction-pipes
#13
Tetsuyou Watanabe, Toshio Koyama, Takeshi Yoneyama, Mitsutoshi Nakada
This paper presents a force-visually-observable silicone retractor, which is an extension of a previously developed system that had the same functions of retracting, suction, and force sensing. These features provide not only high usability by reducing the number of tool changes, but also a safe choice of retracting by visualized force information. Suction is achieved by attaching the retractor to a suction pipe. The retractor has a deformable sensing component including a hole filled with a liquid. The hole is connected to an outer tube, and the liquid level displaced in proportion to the extent of deformation resulting from the retracting load...
April 5, 2017: Sensors
https://www.readbyqxmd.com/read/28377648/comparison-of-antimicrobial-efficacy-of-diode-laser-triphala-and-sodium-hypochlorite-in-primary-root-canals-a-randomized-controlled-trial
#14
Seby Thomas, Sharath Asokan, Baby John, Geetha Priya, S Kumar
AIM: To evaluate the antimicrobial efficacy of diode laser, triphala, and sodium hypochlorite (NaOCl) against Enterococc-cus faecalis contaminated primary root canals. MATERIALS AND METHODS: Forty-nine single-rooted human primary teeth were reduced up to cemento-enamel junction and biomechanically prepared. After sterilization, five teeth were selected as negative controls and remaining teeth were inoculated with E. faecalis. The teeth were then randomly divided into four groups...
January 2017: International Journal of Clinical Pediatric Dentistry
https://www.readbyqxmd.com/read/28375579/seed-vectored-endophytic-bacteria-modulate-development-of-rice-seedlings
#15
S K Verma, K Kingsley, I Irizarry, M Bergen, R N Kharwar, J F White
AIM: The aim of the present study was to evaluate the effects of the removal of indigenous bacteria from rice seeds on seedling growth and development. Here we report the presence of three indigenous endophytic bacteria in rice seeds that play important roles in modulating seedling development (shoot and root lengths, and formation of root hairs and secondary roots) and defence against pathogens. METHODS AND RESULTS: Seed-associated bacteria were removed using surface sterilization with NaOCl (bleach) followed by antibiotic treatment...
April 4, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28372421/an-all-solid-state-microsecond-range-quasi-square-pulse-generator-based-on-fractional-turn-ratio-saturable-pulse-transformer-and-anti-resonance-network
#16
Rong Chen, Jianhua Yang, Xinbing Cheng, Zilong Pan
High voltage pulse generators are widely applied in a number of fields. Defense and industrial applications stimulated intense interests in the area of pulsed power technology towards the system with high power, high repetition rate, solid state characteristics, and compact structure. An all-solid-state microsecond-range quasi-square pulse generator based on a fractional-turn ratio saturable pulse transformer and anti-resonance network is proposed in this paper. This generator consists of a charging system, a step-up system, and a modulating system...
March 2017: Review of Scientific Instruments
https://www.readbyqxmd.com/read/28364327/assessment-of-chlorine-tolerance-profile-of-citrobacter-species-recovered-from-wastewater-treatment-plants-in-eastern-cape-south-africa
#17
Mojisola Owoseni, Anthony Okoh
This present study assessed the chlorine tolerance of some Citrobacter species recovered from secondary effluents from the clarifiers of two wastewater treatment plants in the Eastern Cape, South Africa. The bacterial survival, chlorine lethal dose and inactivation kinetics at lethal doses were examined. Inactivation of the test bacteria (n = 20) at the recommended dose of 0.5 mg/l for 30 min exposure showed a progressive reduction in bacterial population from 4 to 5 log reduction and residuals ranged between 0...
April 2017: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/28364108/-bactericidal-effects-and-smear-layer-removal-of-er-yag-laser-radiation-against-enterococcus-faecalis-in-root-canals
#18
Zongbo Li, Changyun Fang
To evaluate the cleaning ability of Er:YAG laser against Enterococcus faecalis in root canals.
 Methods: The single-rooted human teeth were sterilized and inoculated with Enterococcus faecalis and were randomly assigned into 2 groups. A group of teeth was irrigated with saline during root canal preparation while another group with NaClO. After mechanical preparation, the two groups were randomly divided into 3 subgroups according to the different treatments: Samples, laser radiation, and calcium hydroxide intracanal medication for 7 days...
March 28, 2017: Zhong Nan da Xue Xue Bao. Yi Xue Ban, Journal of Central South University. Medical Sciences
https://www.readbyqxmd.com/read/28362371/a-device-for-performing-cell-migration-wound-healing-in-a-96-well-plate
#19
Po Ying Poon, Patrick Ying Kit Yue, Ricky Ngok Shun Wong
The cell migration/wounding assay is a commonly used method to study cell migration and other biological processes, such as angiogenesis and tumor metastasis. In this assay, cells are grown to form a confluent monolayer and a mechanical wound is created by scratching with a device. Then the migration rate of the cells towards the denuded area can be monitored by imaging. Our 8-channel mechanical wounder is designed to tackle most of the problems associated with the cell migration assay. Firstly, our wounder can be easily sterilized by autoclaving or with common disinfectants...
March 7, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28361272/sterilization-of-blow-fly-eggs-chrysomya-megacephala-and-lucilia-cuprina-diptera-calliphoridae-for-maggot-debridement-therapy-application
#20
Kwankamol Limsopatham, Phadungkiat Khamnoi, Kabkaew L Sukontason, Dheerawan Boonyawan, Tarinee Chaiwong, Kom Sukontason
Maggot debridement therapy (MDT) is an application of sterile laboratory-reared blow fly larvae to remove necrotic tissue and disinfect wounds for medical conditions. For effective application, the blow fly larvae used in the wound treatment are required to be in aseptic condition. Here, we report the results of a detailed assessment of bacteria and fungi isolated from the eggs of two blow fly species, Chrysomya megacephala (F.) and Lucilia cuprina (Wiedemann) before and after sterilization by disinfectants Chlorhex-C, povidone-iodine, and sodium hypochlorite...
March 30, 2017: Parasitology Research
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