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https://www.readbyqxmd.com/read/28640905/an-efficient-method-for-transgenic-callus-induction-from-vitis-amurensis-petiole
#1
Tingting Zhao, Zemin Wang, Lingye Su, Xiaoming Sun, Jun Cheng, Langlang Zhang, Sospeter Karanja Karungo, Yuepeng Han, Shaohua Li, Haiping Xin
Transformation is the main platform for genetic improvement and gene function studies in plants. However, the established somatic embryo transformation system for grapevines is time-consuming and has low efficiency, which limits its utilization in functional genomics research. Vitis amurensis is a wild Vitis species with remarkable cold tolerance. The lack of an efficient genetic transformation system for it has significantly hindered the functional identification of cold stress related genes in the species...
2017: PloS One
https://www.readbyqxmd.com/read/28638880/efficiency-of-borage-seeds-oil-against-gamma-irradiation-induced-hepatotoxicity-in-male-rats-possible-antioxidant-activity
#2
Hala A H Khattab, Inas Z A Abdallah, Fatimah M Yousef, Etimad A Huwait
BACKGROUND: Borage (Borago officinal L.) is an annual herbaceous plant of great interest because its oil contains a high percentage of γ-linolenic acid (GLA). The present work was carried out to detect fatty acids composition of the oil extracted from borage seeds (BO) and its potential effectiveness against γ-irradiation- induced hepatotoxicity in male rats. MATERIALS AND METHODS: GC-MS analysis of fatty acids methyl esters of BO was performed to identify fatty acids composition...
2017: African Journal of Traditional, Complementary, and Alternative Medicines: AJTCAM
https://www.readbyqxmd.com/read/28637218/genome-wide-characterization-and-expression-analysis-enables-identification-of-abiotic-stress-responsive-myb-transcription-factors-in-cassava-manihot-esculenta
#3
Meng-Bin Ruan, Xin Guo, Bin Wang, Yi-Ling Yang, Wen-Qi Li, Xiao-Ling Yu, Peng Zhang, Ming Peng
The myeloblastosis (MYB) transcription factor superfamily is the largest transcription factor family in plants, playing different roles during stress response. However, abiotic stress-responsive MYB transcription factors have not been systematically studied in cassava (Manihot esculenta), an important tropical tuber root crop. In this study, we used a genome-wide transcriptome analysis to predict 299 putative MeMYB genes in the cassava genome. Under drought and cold stresses, many MeMYB genes exhibited different expression patterns in cassava leaves, indicating that these genes might play a role in abiotic stress responses...
June 20, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/28633295/long-cold-exposure-induces-transcriptional-and-biochemical-remodelling-of-xylem-secondary-cell-wall-in-eucalyptus
#4
Raphael Ployet, Marçal Soler, Victor Carocha, Nathalie Ladouce, Ana Alves, José-Carlos Rodrigues, Luc Harvengt, Christiane Marque, Chantal Teulières, Jacqueline Grima-Pettenati, Fabien Mounet
Although eucalypts are the most planted hardwood trees worldwide, the majority of them are frost sensitive. The recent creation of frost-tolerant hybrids such as Eucalyptus gundal plants (E. gunnii × E. dalrympleana hybrids), now enables the development of industrial plantations in northern countries. Our objective was to evaluate the impact of cold on the wood structure and composition of these hybrids, and on the biosynthetic and regulatory processes controlling their secondary cell-wall (SCW) formation...
June 13, 2017: Tree Physiology
https://www.readbyqxmd.com/read/28626940/overexpression-of-hevea-brasiliensis-ethylene-response-factor-hberf-ixc5-enhances-growth-tolerance-to-abiotic-stress-and-affects-laticifer-differentiation
#5
Retno Lestari, Maryannick Rio, Florence Martin, Julie Leclercq, Natthakorn Woraathasin, Sandrine Roques, Florence Dessailly, Anne Clément-Vidal, Christine Sanier, Denis Fabre, Sémi Melliti, Sony Suharsono, Pascal Montoro
Ethylene response factor 1 (ERF1) is an essential integrator of the jasmonate and ethylene signalling pathways coordinating a large number of genes involved in plant defences. Its orthologue in Hevea brasiliensis, HbERF-IXc5, has been assumed to play a major role in laticifer metabolism and tolerance to harvesting stress for better latex production. This paper set out to establish and characterize rubber transgenic lines overexpressing HbERF-IXc5. Overexpression of HbERF-IXc5 dramatically enhanced plant growth and enabled plants to maintain some ecophysiological parameters in response to abiotic stress such as water deficit, cold and salt treatments...
June 19, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28618065/cold-interferes-with-male-meiotic-cytokinesis-in-arabidopsis-thaliana-independently-of-the-ahk2-3-ahp2-3-5-cytokinin-signaling-module
#6
Bing Liu, Nico De Storme, Danny Geelen
Previously we have shown that low temperature stress in Arabidopsis causes defects in microtubule organization and cytokinesis in male meiocytes, which leads to the formation of diploid pollen. Because cytokinin (CK) mediates multiple physiological responses to cold stress, we investigated whether CK signaling is involved in cold-induced diploid pollen formation. To this end, we monitored male sporogenesis in a series of mutants defective in CK metabolism and signalling. Arabidopsis plants with altered CK homeostasis, i...
June 15, 2017: Cell Biology International
https://www.readbyqxmd.com/read/28615006/brief-temperature-stress-during-reproductive-stages-alters-meiotic-recombination-and-somatic-mutation-rates-in-the-progeny-of-arabidopsis
#7
Ramswaroop Saini, Amit Kumar Singh, Shanmuhapreya Dhanapal, Thoufeequl Hakeem Saeed, Geoffrey J Hyde, Ramamurthy Baskar
BACKGROUND: Plants exposed to environmental stresses draw upon many genetic and epigenetic strategies, with the former sometimes modulated by the latter. This can help the plant, and its immediate progeny, at least, to better endure the stress. Some evidence has led to proposals that (epi) genetic changes can be both selective and sustainably heritable, while other evidence suggests that changes are effectively stochastic, and important only because they induce genetic variation. One type of stress with an arguably high level of stochasticity in its effects is temperature stress...
June 14, 2017: BMC Plant Biology
https://www.readbyqxmd.com/read/28603083/overexpressing-ibcbf3-increases-low-temperature-and-drought-stress-tolerance-in-transgenic-sweetpotato
#8
Rong Jin, Beg Hab Kim, Chang Yoon Ji, Ho Soo Kim, Hong Min Li, Dai Fu Ma, Sang-Soo Kwak
Dehydration-responsive element-binding/C-repeat-binding factor (DREB/CBF) proteins regulate the transcription of genes involved in cold acclimation in several species. However, little is known about the physiological functions of CBF proteins in the low temperature-sensitive crop sweetpotato. We previously reported that the DREB1/CBF-like sweetpotato gene SwDREB1/IbCBF3 is involved in responses to diverse abiotic stresses. In this study, we confirmed that IbCBF3 is localized to the nucleus and binds to the C-repeat/dehydration-responsive elements (CRT/DRE) in the promoters of cold-regulated (COR) genes...
June 3, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28596782/chloroplast-or-mitochondria-targeted-dead-box-rna-helicases-play-essential-roles-in-organellar-rna-metabolism-and-abiotic-stress-responses
#9
REVIEW
Ghazala Nawaz, Hunseung Kang
The yields and productivity of crops are greatly diminished by various abiotic stresses, including drought, cold, heat, and high salinity. Chloroplasts and mitochondria are cellular organelles that can sense diverse environmental stimuli and alter gene expression to cope with adverse environmental stresses. Organellar gene expression is mainly regulated at posttranscriptional levels, including RNA processing, intron splicing, RNA editing, RNA turnover, and translational control, during which a variety of nucleus-encoded RNA-binding proteins (RBPs) are targeted to chloroplasts or mitochondria where they play essential roles in organellar RNA metabolism...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28590406/de-novo-transcriptome-sequencing-and-the-hypothetical-cold-response-mode-of-saussurea-involucrata-in-extreme-cold-environments
#10
Jin Li, Hailiang Liu, Wenwen Xia, Jianqiang Mu, Yujie Feng, Ruina Liu, Panyao Yan, Aiying Wang, Zhongping Lin, Yong Guo, Jianbo Zhu, Xianfeng Chen
Saussurea involucrata grows in high mountain areas covered by snow throughout the year. The temperature of this habitat can change drastically in one day. To gain a better understanding of the cold response signaling pathways and molecular metabolic reactions involved in cold stress tolerance, genome-wide transcriptional analyses were performed using RNA-Seq technologies. A total of 199,758 transcripts were assembled, producing 138,540 unigenes with 46.8 Gb clean data. Overall, 184,416 (92.32%) transcripts were successfully annotated...
June 7, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28582694/slcor413im1-a-novel-cold-regulation-gene-from-tomato-enhances-drought-stress-tolerance-in-tobacco
#11
Xiaocui Ma, Guodong Wang, Weiyang Zhao, Minmin Yang, Nana Ma, Fanying Kong, Xinchun Dong, Qingwei Meng
Drought stress adversely affects plant growth, development, and productivity. Genes functioning in plant response to drought stress are essential for drought tolerance. In this study, SlCOR413IM1, a cold-regulated gene isolated from Solanum lycopersium, was transferred to Nicotiana tabacum to investigate its function under drought stress. The subcellular localisation of SlCOR413IM1-GFP fusion protein in Arabidopsis protoplasts suggested that SlCOR413IM1 is a chloroplast protein. Expression analyses revealed that SlCOR413IM1 responded to drought and cold stresses...
March 28, 2017: Journal of Plant Physiology
https://www.readbyqxmd.com/read/28572621/expression-and-integrated-network-analyses-revealed-functional-divergence-of-nhx-type-na-h-exchanger-genes-in-poplar
#12
Fengxia Tian, Ermei Chang, Yu Li, Pei Sun, Jianjun Hu, Jin Zhang
The Na(+)/H(+) antiporters (NHXs) are secondary ion transporters to exchange H(+) and transfer the Na(+) or K(+) across membrane, they play crucial roles during plant development and stress responses. To gain insight into the functional divergence of NHX genes in poplar, eight PtNHX were identified from Populus trichocarpa genome. PtNHXs containing 10 transmembrane helices (TMH) and a hydrophilic C-terminal domain, the TMH compose a hollow cylinder to provide the channel for Na(+) and H(+) transport. The expression patterns and cis-acting elements showed that all the PtNHXs were response to single or multiple stresses including drought, heat, cold, salinity, MV, and ABA...
June 1, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28562633/bioinformatics-identification-and-transcript-profile-analysis-of-the-mitogen-activated-protein-kinase-gene-family-in-the-diploid-woodland-strawberry-fragaria-vesca
#13
Wei Wei, Zhuangzhuang Chai, Yinge Xie, Kuan Gao, Mengyuan Cui, Ying Jiang, Jiayue Feng
Mitogen-activated protein kinases (MAPKs) play essential roles in mediating biotic and abiotic stress responses in plants. However, the MAPK gene family in strawberry has not been systematically characterized. Here, we performed a genome-wide survey and identified 12 MAPK genes in the Fragaria vesca genome. Protein domain analysis indicated that all FvMAPKs have typical protein kinase domains. Sequence alignments and phylogenetic analysis classified the FvMAPK genes into four different groups. Conserved motif and exon-intron organization supported the evolutionary relationships inferred from the phylogenetic analysis...
2017: PloS One
https://www.readbyqxmd.com/read/28559903/expression-of-cddhn4-a-novel-ysk2-type-dehydrin-gene-from-bermudagrass-responses-to-drought-stress-through-the-aba-dependent-signal-pathway
#14
Aimin Lv, Nana Fan, Jianping Xie, Shili Yuan, Yuan An, Peng Zhou
Dehydrin improves plant resistance to many abiotic stresses. In this study, the expression profiles of a dehydrin gene, CdDHN4, were estimated under various stresses and abscisic acid (ABA) treatments in two bermudagrasses (Cynodon dactylon L.): Tifway (drought-tolerant) and C299 (drought-sensitive). The expression of CdDHN4 was up-regulated by high temperatures, low temperatures, drought, salt and ABA. The sensitivity of CdDHN4 to ABA and the expression of CdDHN4 under drought conditions were higher in Tifway than in C299...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28558066/gene-expression-analysis-of-rocket-salad-under-pre-harvest-and-postharvest-stresses-a-transcriptomic-resource-for-diplotaxis-tenuifolia
#15
Marina Cavaiuolo, Giacomo Cocetta, Natasha Damiana Spadafora, Carsten T Müller, Hilary J Rogers, Antonio Ferrante
Diplotaxis tenuifolia L. is of important economic value in the fresh-cut industry for its nutraceutical and sensorial properties. However, information on the molecular mechanisms conferring tolerance of harvested leaves to pre- and postharvest stresses during processing and shelf-life have never been investigated. Here, we provide the first transcriptomic resource of rocket by de novo RNA sequencing assembly, functional annotation and stress-induced expression analysis of 33874 transcripts. Transcriptomic changes in leaves subjected to commercially-relevant pre-harvest (salinity, heat and nitrogen starvation) and postharvest stresses (cold, dehydration, dark, wounding) known to affect quality and shelf-life were analysed 24h after stress treatment, a timing relevant to subsequent processing of salad leaves...
2017: PloS One
https://www.readbyqxmd.com/read/28555319/proteomic-analysis-of-rice-seedlings-under-cold-stress
#16
Li Ji, Ping Zhou, Ya Zhu, Fang Liu, Rongbai Li, Yongfu Qiu
Low temperature can greatly restrict the growth and development of rice. The rice seedlings show growth retardation, lamina wrap, and part of blade even died under the condition of low temperature. In order to get more information about cold stress responses in rice, two dimensional electrophoresis and bioinformatics analysis of mass spectrometry were used to preliminary survey the cold tolerance of cold sensitive line 9311 and cold resistance variety Fujisaka 5 under cold stress. Two dimensional electrophoresis maps of 9311 and Fujisaka 5 were established under cold treatment...
May 29, 2017: Protein Journal
https://www.readbyqxmd.com/read/28554480/sgd1-a-key-enzyme-in-tocopherol-biosynthesis-is-essential-for-plant-development-and-cold-tolerance-in-rice
#17
Di Wang, Yunlong Wang, Wuhua Long, Mei Niu, Zhigang Zhao, Xuan Teng, Xiaopin Zhu, Jianping Zhu, Yuanyuan Hao, Yongfei Wang, Yi Liu, Ling Jiang, Yihua Wang, Jianmin Wan
Tocopherols, a group of Vitamin E compounds, are essential components of the human diet. In contrast to well documented roles in animals, the functions of tocopherols in plants are less understood. In this study, we characterized two allelic rice dwarf mutant lines designated sgd1-1 and sgd1-2 (small grain and dwarf1). Histological observations showed that the dwarf phenotypes were mainly due to cell elongation defects. A map-based cloning strategy and subsequent complementation test showed that SGD1 encodes homogentisate phytyltransferase (HPT), a key enzyme in tocopherol biosynthesis...
July 2017: Plant Science: An International Journal of Experimental Plant Biology
https://www.readbyqxmd.com/read/28553310/effects-of-melatonin-on-anti-oxidative-systems-and-photosystem-ii-in-cold-stressed-rice-seedlings
#18
Qiao-Hong Han, Bo Huang, Chun-Bang Ding, Zhong-Wei Zhang, Yang-Er Chen, Chao Hu, Li-Jun Zhou, Yan Huang, Jin-Qiu Liao, Shu Yuan, Ming Yuan
Melatonin (N-acetyl-5-methoxytryptamine) plays important role in multiple plant developmental processes and stress responses. We investigated the possible mediatory role of melatonin in growth, photosynthesis, and the response to cold stress in rice by using three different experiments: soaking seed; immersing roots, and spraying to leaves with 0, 20, or 100 μM melatonin. After 6 days of cold stress, the growth of rice seedlings was significantly inhibited, but this inhibition was alleviated by exogenous melatonin...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28551420/an-analysis-of-the-development-of-cauliflower-seed-as-a-model-to-improve-the-molecular-mechanism-of-abiotic-stress-tolerance-in-cauliflower-artificial-seeds
#19
Hail Z Rihan, Mohammed Al-Issawi, Michael P Fuller
The development stages of conventional cauliflower seeds were studied and the accumulation of dehydrin proteins through the maturation stages was investigated with the aim of identifying methods to improve the viability of artificial seeds of cauliflower. While carbohydrate, ash and lipids increased throughout the development of cauliflower traditional seeds, proteins increased with the development of seed and reached the maximum level after 75 days of pollination, however, the level of protein started to decrease after that...
May 22, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28548656/nlr-locus-mediated-trade-off-between-abiotic-and-biotic-stress-adaptation-in-arabidopsis
#20
Hirotaka Ariga, Taku Katori, Takashi Tsuchimatsu, Taishi Hirase, Yuri Tajima, Jane E Parker, Rubén Alcázar, Maarten Koornneef, Owen Hoekenga, Alexander E Lipka, Michael A Gore, Hitoshi Sakakibara, Mikiko Kojima, Yuriko Kobayashi, Satoshi Iuchi, Masatomo Kobayashi, Kazuo Shinozaki, Yoichi Sakata, Takahisa Hayashi, Yusuke Saijo, Teruaki Taji
Osmotic stress caused by drought, salt or cold decreases plant fitness. Acquired stress tolerance defines the ability of plants to withstand stress following an initial exposure(1). We found previously that acquired osmotolerance after salt stress is widespread among Arabidopsis thaliana accessions(2). Here, we identify ACQOS as the locus responsible for ACQUIRED OSMOTOLERANCE. Of its five haplotypes, only plants carrying group 1 ACQOS are impaired in acquired osmotolerance. ACQOS is identical to VICTR, encoding a nucleotide-binding leucine-rich repeat (NLR) protein(3)...
May 26, 2017: Nature Plants
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