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Streptococcus salivarius

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https://www.readbyqxmd.com/read/28747897/identification-of-emerging-human-mastitis-pathogens-by-maldi-tof-and-assessment-of-their-antibiotic-resistance-patterns
#1
María Marín, Rebeca Arroyo, Irene Espinosa-Martos, Leónides Fernández, Juan M Rodríguez
Lactational mastitis constitutes one of the main causes of undesired weaning, depriving the mother-infant pair from the benefits of breastfeeding; therefore, this condition should be considered a relevant public health issue. The role of specific microorganisms remains unclear since human milk cultures and antibiotic susceptibility testing (AST) are not routinely performed, despite the fact that this would be key to ensure an early and effective diagnosis and treatment. The objective of this study was to describe the culturable microbial diversity in 647 milk samples from breastfeeding women with clinical symptoms of mastitis by Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF) VITEK MS technology and to analyze the antimicrobial susceptibility profiles of a collection of isolates from these samples by the VITEK 2 AST system...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28740363/synthesis-and-antimicrobial-activity-of-chiral-quaternary-n-spiro-ammonium-bromides-with-3-4-dihydro-1-h-spiro-isoindoline-2-2-isoquinoline-skeleton
#2
Krzysztof Bielawski, Katarzyna Leszczyńska, Zbigniew Kałuża, Anna Bielawska, Olga Michalak, Tamara Daniluk, Olga Staszewska-Krajewska, Anna Czajkowska, Natalia Pawłowska, Agnieszka Gornowicz
A new class of highly functionalized tetrahydroisoquinolines with a quaternary carbon stereocenter was synthesized starting from an easily accessible L-tartaric acid. Nine strains of bacteria (Staphylococcus aureus, Streptococcus pyogenes, Streptococcus mutans, Streptococcus salivarius, Bacillus subtilis, Enterococcus faecalis, Moraxella catarrhalis, Escherichia coli, Campylobacter jejuni) were used for the determination of minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of synthesized compounds...
2017: Drug Design, Development and Therapy
https://www.readbyqxmd.com/read/28729683/a-positive-feedback-loop-mediated-by-sigma-x-enhances-expression-of-the-streptococcal-regulator-comr
#3
Rabia Khan, Roger Junges, Heidi A Åmdal, Tsute Chen, Donald A Morrison, Fernanda C Petersen
Natural transformation is used by bacteria to take up DNA from their surroundings and incorporate it into their genomes. Streptococci do so during a transient period of competence, triggered by pheromones that they produce, secrete and sense under conditions influenced by the environment. In Streptococcus mutans, Streptococcus suis, and species of the bovis, salivarius and pyogenic groups of streptococci, the pheromone XIP is sensed by the intra-cellular regulator ComR, that in turn activates the transcription of comS, encoding the XIP precursor, and of sigX, encoding the only known alternative sigma factor in streptococci...
July 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28695648/three-glycosylated-serine-rich-repeat-proteins-play-a-pivotal-role-in-adhesion-and-colonization-of-the-pioneer-commensal-bacterium-streptococcus-salivarius
#4
Benoit Couvigny, Nicolas Lapaque, Lionel Rigottier-Gois, Alain Guillot, Sophie Chat, Thierry Meylheuc, Saulius Kulakauskas, Manfred Rohde, Michel-Yves Mistou, Pierre Renault, Joel Doré, Romain Briandet, Pascale Serror, Eric Guédon
Bacterial adhesion is a critical step for colonization of the host. The pioneer colonizer and commensal bacterium of the human gastrointestinal tract, Streptococcus salivarius, has strong adhesive properties but the molecular determinants of this adhesion remain uncharacterized. Serine-rich repeat (SRR) glycoproteins are a family of adhesins that fulfill an important role in adhesion. In general, Gram-positive bacterial genomes have a unique SRR glycoprotein-encoding gene. We demonstrate that S. salivarius expresses three large and glycosylated surface-exposed proteins - SrpA, SrpB and SrpC - that show characteristics of SRR glycoproteins and are secreted through the accessory SecA2/Y2 system...
July 11, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/28691028/production-of-a-functional-frozen-yogurt-fortified-with-bifidobacterium-spp
#5
Amro Abdelazez, Zafarullah Muhammad, Qiu-Xue Zhang, Zong-Tao Zhu, Heba Abdelmotaal, Rokayya Sami, Xiang-Chen Meng
Frozen dairy products have characteristics of both yogurt and ice cream and could be the persuasive carriers of probiotics. Functions of the frozen yogurt containing viable bifidobacterial cells are recognized and favored by the people of all ages. We developed a kind of yogurt supplemented by Bifidobacterium species. Firstly, five strains of Bifidobacterium spp. (Bifidobacterium bifidum ATCC 11547, Bifidobacterium longum ATCC 11549, Bifidobacterium infantis ATCC 11551, Bifidobacterium adolescentis ATCC 11550, and Bifidobacterium breve ATCC 11548) were evaluated based on the feasibility criteria of probiotics, comprising acid production, bile tolerance, and adhesion to epithelial cells...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28684920/bacteriotherapy-with-streptococcus-salivarius-24smb-and-streptococcus-oralis-89a-nasal-spray-for-preventing-recurrent-acute-otitis-media-in-children-a-real-life-clinical-experience
#6
Ignazio La Mantia, Attilio Varricchio, Giorgio Ciprandi
Recurrence of acute otitis media (RAOM) is a relevant issue in the clinical practice. "Bacteriotherapy" has been proposed as an option in children with RAOM. Streptococcus salivarius 24SMB nasal spray has been previously demonstrated to reduce the risk of acute otitis media (AOM) in otitis-prone children. The current retrospective and observational study aimed to confirm this outcome in a real-life setting, such as the common pediatric practice. Group A (108 children) served as control; group B (159 children) was treated with S...
2017: International Journal of General Medicine
https://www.readbyqxmd.com/read/28681177/efficacy-and-safety-of-probiotic-supplemented-triple-therapy-for-eradication-of-helicobacter-pylori-in-children-a-systematic-review-and-network-meta-analysis
#7
REVIEW
Jue-Rong Feng, Fan Wang, Xiao Qiu, Lynne V McFarland, Peng-Fei Chen, Rui Zhou, Jing Liu, Qiu Zhao, Jin Li
AIM: The aim of this study was to identify the best probiotic supplementation in triple therapy for pediatric population with Helicobacter pylori infection. METHODS: Eligible trials were identified by comprehensive searches. Relative risks with 95% confidence intervals and relative ranks with P scores were assessed. RESULTS: Twenty-nine trials (3122 participants) involving 17 probiotic regimens were identified. Compared with placebo, probiotic-supplemented triple therapy significantly increased H...
July 5, 2017: European Journal of Clinical Pharmacology
https://www.readbyqxmd.com/read/28675006/-construction-of-a-low-ph-sensing-system-in-streptococcus-mutans
#8
Kang Di, Li Yuqing, Zhou Xuedong
OBJECTIVE: To construct a low-pH-sensing system in Streptococcus mutans (S. mutans) and to visually detect the pH in situ. METHODS: Promoter of ureaseⅠ(PureⅠ) and green fluorescence protein (gfp) DNA fragments were amplified by polymerase chain reaction (PCR) from the genome of Streptococcus salivarius 57.I and S. mutans containing the gfp fragment. The two amplified DNA fragments were ligated together and further integrated into pDL278 to construct the recombinant plasmid pDL278-pureⅠ-gfp...
June 1, 2017: Hua Xi Kou Qiang Yi Xue za Zhi, Huaxi Kouqiang Yixue Zazhi, West China Journal of Stomatology
https://www.readbyqxmd.com/read/28658918/efficacy-of-neem-extract-and-three-antimicrobial-agents-incorporated-into-tissue-conditioner-in-inhibiting-the-growth-of-c-albicans-and-s-mutans
#9
Dikshita Ray Barua, Jayaprakash Mugur Basavanna, Rana Kalappattil Varghese
INTRODUCTION: Denture stomatitis is an inflammatory condition which compromises the mucosal surface beneath dentures. The aetiology of denture stomatitis is usually multifactorial which varies from trauma from ill fitting denture to poor immune system. There are evidences that denture stomatitis is an outcome of multispecies biofilms that include Candida albicans and Streptococcus mutans. Tissue conditioners are found to be more susceptible to colonisation by micro-organisms. AIM: The purpose of this study was to compare the efficacy of neem leaf extract and three other antimicrobial agents incorporated in a tissue conditioner against both Candida albicans and Streptococcus mutans...
May 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28658911/effect-of-green-coffee-bean-extract-on-streptococcus-mutans-count-a-randomised-control-trial
#10
Mona Yadav, Mamta Kaushik, Roshni Roshni, Pallavi Reddy, Neha Mehra, Vallari Jain, Ritu Rana
INTRODUCTION: Mouth rinses have been popularly used as a supplementary oral hygiene aid. A lot of commercially available mouth rinses possess few adverse effects, which has necessitated the search for alternative and herbal mouth rinses. AIM: The aim of the study was to assess the effect of rinsing with green coffee bean extract in comparison with chlorhexidine mouthwash and sterile water on salivary Streptococcus mutans count. MATERIALS AND METHODS: A randomized parallel controlled clinical trial was planned and 45 subjects aged between 18-22 years were selected...
May 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28650782/effect-of-probiotic-yogurt-and-xylitol-containing-chewing-gums-on-salivary-s-mutans-count
#11
Elnaz Ghasemi, Romina Mazaheri, Arezoo Tahmourespour
BACKGROUND AND AIMS: In addition to improving gastrointestinal health and intestinal microflora, probiotic bacteria have been recently suggested to decrease cariogenic agents in the oral cavity. The aim of this study was to investigate the effects of probiotic yogurt and xylitol-containing chewing gums on reducing salivary Streptococcus mutans levels. STUDY DESIGN: This randomized clinical trial recruited 50 female students with over 10(5) colony forming units S...
2017: Journal of Clinical Pediatric Dentistry
https://www.readbyqxmd.com/read/28619805/complete-genome-sequences-of-two-human-oral-microbiome-commensals-streptococcus-salivarius-atcc-25975-and-s-salivarius-atcc-27945
#12
Robert R Butler, Jahna T A Soomer-James, Michel Frenette, Jean-François Pombert
Streptococcus salivarius strains are significant contributors to the human oral microbiome. Some possess unique fimbriae that give them the ability to coaggregate and colonize particular oral structures. We present here the complete genomes of Streptococcus salivarius Lancefield K(-)/K(+) strains ATCC 25975 and ATCC 27945, which can and cannot, respectively, produce fimbriae.
June 15, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28571283/estimation-of-dmft-salivary-streptococcus-mutans-count-flow-rate-ph-and-salivary-total-calcium-content-in-pregnant-and-non-pregnant-women-a-prospective-study
#13
Wasim Ismail Kamate, Nupura Aniket Vibhute, Rajendra Krishna Baad
INTRODUCTION: Pregnancy, a period from conception till birth, causes changes in the functioning of the human body as a whole and specifically in the oral cavity that may favour the emergence of dental caries. Many studies have shown pregnant women at increased risk for dental caries, however, specific salivary caries risk factors and the particular period of pregnancy at heightened risk for dental caries are yet to be explored and give a scope of further research in this area. AIM: The aim of the present study was to assess the severity of dental caries in pregnant women compared to non-pregnant women by evaluating parameters like Decayed, Missing, Filled Teeth (DMFT) index, salivary Streptococcus mutans count, flow rate, pH and total calcium content...
April 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28566853/bacteriostatic-effect-of-simvastatin-on-selected-oral-streptococci-in-vitro
#14
Eugene J Whitaker, Abdulaziz Alshammari
CONTEXT AND OBJECTIVE: Simvastatin is a widely used cholesterol-lowering drug, which has been found to have a number of pleiotropic effects. The aim of this study was to evaluate the antimicrobial effectiveness of simvastatin against selected oral streptococci as determined by the minimum inhibitory concentration (MIC). METHODS: Streptococcus mutans, Streptococcus sanguis, Streptococcus anginosus, and Streptococcus salivarius were the test microorganisms. The serial dilution method was used to determine the MIC of simvastatin against these organisms...
January 2017: Contemporary Clinical Dentistry
https://www.readbyqxmd.com/read/28558854/probiotic-yogurt-and-acidified-milk-similarly-reduce-postprandial-inflammation-and-both-alter-the-gut-microbiota-of-healthy-young-men
#15
Kathryn J Burton, Marta Rosikiewicz, Grégory Pimentel, Ueli Bütikofer, Ueli von Ah, Marie-Jeanne Voirol, Antony Croxatto, Sébastien Aeby, Jocelyne Drai, Philip G McTernan, Gilbert Greub, François P Pralong, Guy Vergères, Nathalie Vionnet
Probiotic yogurt and milk supplemented with probiotics have been investigated for their role in 'low-grade' inflammation but evidence for their efficacy is inconclusive. This study explores the impact of probiotic yogurt on metabolic and inflammatory biomarkers, with a parallel study of gut microbiota dynamics. The randomised cross-over study was conducted in fourteen healthy, young men to test probiotic yogurt compared with milk acidified with 2 % d-(+)-glucono-δ-lactone during a 2-week intervention (400 g/d)...
May 2017: British Journal of Nutrition
https://www.readbyqxmd.com/read/28543778/oral-flora-in-acute-stroke-patients-a-prospective-exploratory-observational-study
#16
Elizabeth Boaden, Mary Lyons, Sim K Singhrao, Hazel Dickinson, Michael Leathley, Catherine E Lightbody, Alison McLoughlin, Zarine Khan, Stjohn Crean, Craig Smith, Susan Higham, Caroline Watkins
OBJECTIVE: To describe the bacterial profile of the oral flora during the first 2 weeks following a stroke, examining changes in the condition of the oral cavity and infections. BACKGROUND: Dysphagia is common after a stroke and can lead to aspiration pneumonia. Oral flora changes associated with stroke have been implicated as a possible source of bacteria that can cause systemic infections. MATERIALS AND METHODS: Seventy-seven participants were recruited over a period of 9 months...
May 22, 2017: Gerodontology
https://www.readbyqxmd.com/read/28512279/antimicrobial-potential-of-plant-extracts-and-chemical-fractions-of-sideroxylon-obtusifolium-roem-schult-t-d-penn-on-oral-microorganisms
#17
Thaíse Pd Sampaio, Nathália Ao Cartaxo-Furtado, Ana Cd de Medeiros, Harley S Alves, Pedro L Rosalen, Jozinete V Pereira
AIM: The aim of this study is to evaluate the in vitro antimicrobial activity of plant extracts and chemical fractions of Sideroxylon obtusifolium T.D. Penn on Streptococcus mutans, Streptococcus oralis, Streptococcus salivarius, Streptococcus parasanguinis, and Candida albicans as well as to identify the chemical classes found in the bioactive extracts possessing better activity. MATERIALS AND METHODS: Freeze-dried hydroalcoholic extracts of the bark and leaves (LC and LF respectively) and ethanol extracts of bark and leaves (EC and EF respectively) of S...
May 1, 2017: Journal of Contemporary Dental Practice
https://www.readbyqxmd.com/read/28507066/the-microbiome-of-site-specific-dental-plaque-of-children-with-different-caries-status
#18
Vincent P Richards, Andres J Alvarez, Amy R Luce, Molly Bedenbaugh, Mary Mitchell, Robert A Burne, Marcelle M Nascimento
The oral microbiota associated with the initiation and progression of dental caries has yet to be fully characterized. The Human Oral Microbe Identification using Next Generation Sequencing (HOMINGS) was used to analyze the microbiomes of site-specific supragingival dental plaques from children with different caries status. Fifty-five children (2-7 years) were assessed at baseline and 12-months, and grouped as: caries-free (CF), caries-active with enamel lesions (CAE), and caries-active with dentin carious lesions (CA)...
May 15, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28486933/influence-of-chronic-azithromycin-treatment-on-the-composition-of-the-oropharyngeal-microbial-community-in-patients-with-severe-asthma
#19
Guido Lopes Dos Santos Santiago, Guy Brusselle, Kenny Dauwe, Pieter Deschaght, Chris Verhofstede, Dries Vaneechoutte, Ellen Deschepper, Paul Jordens, Guy Joos, Mario Vaneechoutte
BACKGROUND: This study of the oropharyngeal microbiome complements the previously published AZIthromycin in Severe ASThma (AZISAST) clinical trial, where the use of azithromycin was assessed in subjects with exacerbation-prone severe asthma. Here, we determined the composition of the oropharyngeal microbial community by means of deep sequencing of the amplified 16S rRNA gene in oropharyngeal swabs from patients with exacerbation-prone severe asthma, at baseline and during and after 6 months treatment with azithromycin or placebo...
May 10, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28432093/diversity-of-integrative-and-conjugative-elements-of-streptococcus-salivarius-and-their-intra-and-interspecies-transfer
#20
Narimane Dahmane, Virginie Libante, Florence Charron-Bourgoin, Eric Guédon, Gérard Guédon, Nathalie Leblond-Bourget, Sophie Payot
Integrative and conjugative elements (ICEs) are widespread chromosomal mobile genetic elements which can transfer autonomously by conjugation in bacteria. Thirteen ICEs with a conjugation module closely related to that of ICESt3 of Streptococcus thermophilus were characterized in Streptococcus salivarius by whole-genome sequencing. Sequence comparison highlighted ICE evolution by shuffling of 3 different integration/excision modules (for integration in the 3' end of the fda, rpsI, or rpmG gene) with the conjugation module of the ICESt3 subfamily...
July 1, 2017: Applied and Environmental Microbiology
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