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Streptococcus salivarius

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https://www.readbyqxmd.com/read/28194750/differential-responses-of-human-dendritic-cells-to-metabolites-from-the-oral-airway-microbiome
#1
Katrine Whiteson, Sudhanshu Agrawal, Anshu Agrawal
Small molecule metabolites that are produced or altered by host-associated microbial communities are emerging as significant immune response modifiers. However, there is a key gap in our knowledge of how oral microbial metabolites affect the immune response. Here, we examined the effects of metabolites from five bacterial strains commonly found in the oral/airway microbial communities of humans. The five strains, each isolated from cystic fibrosis patient sputum, were Pseudomonas aeruginosa FLR01 non-mucoid (P1) and FLR02 mucoid (P2) forms, Streptococcus pneumoniae (Sp), Streptococcus salivarius (Ss), and Rothia mucilaginosa (Rm)...
February 14, 2017: Clinical and Experimental Immunology
https://www.readbyqxmd.com/read/28187188/development-of-a-flow-chamber-system-for-the-reproducible-in-vitro-analysis-of-biofilm-formation-on-implant-materials
#2
Henryke Rath, Sascha Nico Stumpp, Meike Stiesch
Since the introduction of modern dental implants in the 1980s, the number of inserted implants has steadily increased. Implant systems have become more sophisticated and have enormously enhanced patients' quality of life. Although there has been tremendous development in implant materials and clinical methods, bacterial infections are still one of the major causes of implant failure. These infections involve the formation of sessile microbial communities, called biofilms. Biofilms possess unique physical and biochemical properties and are hard to treat conventionally...
2017: PloS One
https://www.readbyqxmd.com/read/28165440/antimicrobial-efficacy-of-probiotic-containing-toothpastes-an-in-vitro-evaluation
#3
Ivana Parčina Amižić, Livia Cigić, Lidia Gavić, Marina Radić, Dolores Biočina Lukenda, Marija Tonkić, Ivana Goić Barišić
Aim To evaluate, in vitro antimicrobial ability of two probiotic toothpastes (one containing Lactobacillus paracasei, other containing Lactobacillus acidophilus) and one toothpaste without probiotic separately, and in a combination with two different mouthrinses (one containing essential oils and the other containing hexitidine). Methods Antimicrobial susceptibility was checked by using the ditch method and Clinical Laboratory Standard Institute (CLSI) guidelines. Two different toothpastes with probiotic, toothpaste without probiotic and two different mouthrinses were tested against the following selected microorganisms: Candida albicans, Candida tropicalis, Enterococcus faecalis, Streptococcus salivarius and Staphylococcus aureus...
February 1, 2017: Medicinski Glasnik
https://www.readbyqxmd.com/read/28144393/percutaneous-drainage-as-a-first-therapeutic-step-prior-to-surgery-in-liver-hydatid-cyst-abscess-is-it-worth-it
#4
Aylhin J Lopez-Marcano, Jose M Ramia, Vladimir Arteaga, Roberto De la Plaza, Jhonny D Gonzales, Anibal Medina
AIM: To delay surgery until the patient is in a better condition, and thus to decrease postoperative morbidity. METHODS: Using this algorithm we treated three patients aged 55, 75 and 80 years. In all three patients the clinical presentation was fever without a clear source of infection; all had nonspecific symptoms such as general malaise, dyspnea, and abdominal discomfort in the previous 15 d. They came to the emergency room at our hospital due to deterioration of their general condition...
January 18, 2017: World Journal of Hepatology
https://www.readbyqxmd.com/read/28139479/antibacterial-effect-and-physical-properties-of-chitosan-and-chlorhexidine-cetrimide-modified-glass-ionomer-cements
#5
Apurva Mishra, Ramesh Kumar Pandey, Natesan Manickam
AIMS: To compare antibacterial effect and physical properties of chitosan (CH) modified glass ionomer cement (GIC) (10% v/v), chlorhexidine-cetrimide (CHX-CT) modified GIC (2.5/2.5% w/w) and conventional GIC. MATERIALS AND METHODS: A total of fifty healthy children of age 7-12 years were selected and randomly assigned to class A and B for in vivo analysis. Slabs of CH modified GIC (Group II) along with slabs of conventional GIC (Group I, control) were cemented on buccal surfaces of maxillary molars (split-mouth technique) for class A children...
January 2017: Journal of the Indian Society of Pedodontics and Preventive Dentistry
https://www.readbyqxmd.com/read/28135680/biofunctional-polyethylene-glycol-coatings-on-titanium-an-in-vitro-based-comparison-of-functionalization-methods
#6
Judit Buxadera-Palomero, Cristina Calvo, Sergi Torrent-Camarero, F Javier Gil, Carlos Mas-Moruno, Cristina Canal, Daniel Rodríguez
Three methods for the production of Polyethylene glycol (PEG) coatings on titanium are compared, i.e. plasma polymerization, electrodeposition and silanization. The compared deposition methods presented similar wettability (hydrophilic coatings), chemical composition assessed by XPS and thickness around 1nm. The coatings lowered albumin adsorption and presented a decreased fibroblast, Streptococcus sanguinis and Lactobacillus salivarius adhesion. Immobilization of a cell adhesion peptide (RGD) presented a higher fibroblast adhesion and no alteration of the bacterial adhesion, giving three methods for the biofunctionalization of titanium for dental implants...
January 24, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28127162/comparative-evaluation-of-0-2-chlorhexidine-mouthwash-xylitol-chewing-gum-and-combination-of-0-2-chlorhexidine-mouthwash-and-xylitol-chewing-gum-on-salivary-streptococcus-mutans-and-biofilm-levels-in-8-to-12-year-old-children
#7
Meena Syed, Radhika Chopra, Vandana Shrivastava, Vinod Sachdev
AIM: To assess the effect of combining 0.2% chlorhexidine (CHX) mouthwash with xylitol (XYL) chewing gum on Streptococcus mutans and biofilm levels among 8- to 12-year-old children. MATERIALS AND METHODS: Sixty children aged 8 to 12 years were selected with moderate and high salivary S. mutans levels. They were divided into three groups of 20 children each: (1) XYL group where the subjects chewed XYL twice daily; (2) CHX where rinsing was done twice daily; and (3) combination of XYL and CHX group (XYL+CHX) where both the agents were used once daily...
October 2016: International Journal of Clinical Pediatric Dentistry
https://www.readbyqxmd.com/read/28070022/stable-isotope-profiles-reveal-active-production-of-vocs-from-human-associated-microbes
#8
Joann Phan, Simone Meinardi, Barbara Barletta, Donald Blake, Katrine Whiteson
Volatile organic compounds (VOCs) measured from exhaled breath have great promise for the diagnosis of bacterial infections. However, determining human or microbial origin of VOCs detected in breath remains a great challenge. For example, the microbial fermentation product 2,3-butanedione was recently found in the breath of CF patients; parallel culture-independent metagenomic sequencing of the same samples revealed that Streptococcus and Rothia spp. have the genetic capacity to produce 2,3-butanedione. To investigate whether the genetic capacity found in metagenomes translates to bacterial production of a VOC of interest such as 2,3-butanedione, we fed stable isotopes to three bacterial strains isolated from Cystic Fibrosis (CF) patients: two Gram-positive bacteria, Rothia mucilaginosa and Streptococcus salivarius, and a dominant opportunistic Gram-negative pathogen, Pseudomonas aeruginosa...
January 10, 2017: Journal of Breath Research
https://www.readbyqxmd.com/read/28062463/ymc-2011-a-temperate-phage-of-streptococcus-salivarius-57-i
#9
Wen-Chun Chou, Szu-Chuan Huang, Cheng-Hsun Chiu, Yi-Ywan M Chen
: Streptococcus salivarius is an abundant isolate of the oral cavity. The genome of S. salivarius 57.I consists of a 2-Mb chromosome and a 40,758-bp circular molecule, designated YMC-2011. Annotation of YMC-2011 revealed 55 open reading frames, most of them associated with phage production, although plaque formation is not observed in S. salivarius 57.I after lytic induction using mitomycin C. Results from Southern hybridization and quantitative real-time PCR confirmed that YMC-2011 exists extrachromosomally with an estimated copy number of 3 to 4...
January 6, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28029097/influence-of-silver-content-on-rifampicin-adsorptivity-for-magnetite-ag-rifampicin-nanoparticles
#10
Olena Ivashchenko, Emerson Coy, Barbara Peplinska, Marcin Jarek, Mikołaj Lewandowski, Karol Załęski, Alicja Warowicka, Anna Wozniak, Tatiana Babutina, Justyna Jurga-Stopa, Janez Dolinsek, Stefan Jurga
Magnetite nanoparticles (NPs) decorated with silver (magnetite/Ag) are intensively investigated due to their application in the biomedical field. We demonstrate that the increase of silver content on the surface of nanoparticles improves the adsorptivity of antibiotic rifampicin as well as antibacterial properties. The use of ginger extract allowed to improve the silver nucleation on the magnetite surface that resulted in an increase of silver content. Physicochemical and functional characterization of magnetite/Ag NPs was performed...
February 3, 2017: Nanotechnology
https://www.readbyqxmd.com/read/27999400/comparative-study-on-the-characteristics-of-weissella-cibaria-cmu-and-probiotic-strains-for-oral-care
#11
Hye-Jin Jang, Mi-Sun Kang, Sung-Hun Yi, Ji-Young Hong, Sang-Pil Hong
Probiotics have been demonstrated as a new paradigm to substitute antibiotic treatment for dental caries, gingivitis, and chronic periodontitis. The present work was conducted to compare the characteristics of oral care probiotics: Weissella cibaria CMU (Chonnam Medical University) and four commercial probiotic strains. Survival rates under poor oral conditions, acid production, hydrogen peroxide production, as well as inhibition of biofilm formation, coaggregation, antibacterial activity, and inhibition of volatile sulfur compounds were evaluated...
December 20, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27994423/assessment-of-streptococcus-mutans-in-healthy-versus-gingivitis-and-chronic-periodontitis-a-clinico-microbiological-study
#12
Sneha Dani, Ashwin Prabhu, K R Chaitra, N C Desai, Sudhir R Patil, Ranjan Rajeev
BACKGROUND: Dental caries and periodontal disease are most common oral diseases. Streptococcus mutans are considered to be the major pathogens in initiation of dental caries. Evidence shows that periodontal disease and caries share a number of contributory factors. Thus in view of these findings it would be worthwhile to examine whether Streptococcus mutans persist within the saliva and subgingival environment of the periodontitis patients and to determine whether there is any association between Streptococcus mutans colonization, pH of saliva and sub-gingival plaque pH in periodontal diseases before therapy...
October 2016: Contemporary Clinical Dentistry
https://www.readbyqxmd.com/read/27932242/elevated-salivary-iga-decreased-anxiety-and-an-altered-oral-microbiota-are-associated-with-active-participation-on-an-undergraduate-athletic-team
#13
Ashley L Lamb, Debra E Hess, Sherie Edenborn, Elizabeth Ubinger, Andres E Carrillo, Pierette M Appasamy
BACKGROUND: Previous reports indicate that regular, but not excessive, exercise can moderate the response to anxiety and alter the immune response, therefore we hypothesized that college student athletes who were actively participating on an NCAA Division III athletics team ("in-season") would have lower levels of anxiety and higher salivary IgA levels than similar college athletes who were in their "off-season". NCAA Division III athletes participate in athletics at a level of intensity that is more moderate compared to other NCAA divisions...
February 1, 2017: Physiology & Behavior
https://www.readbyqxmd.com/read/27924597/natural-transformation-of-oral-streptococci-by-use-of-synthetic-pheromones
#14
Gabriela Salvadori, Roger Junges, Rabia Khan, Heidi A Åmdal, Donald A Morrison, Fernanda C Petersen
The discovery that Streptococcus pneumoniae uses a competence-stimulating peptide (CSP) to induce competence for natural transformation, and that other species of the mitis and the anginosus streptococcal groups use a similar system, has expanded the tools to explore gene function and regulatory pathways in streptococci. Two other classes of pheromones have been discovered since then, comprising the bacteriocin-inducing peptide class found in Streptococcus mutans (also named CSP, although different from the former) and the SigX-inducing peptides (XIP), in the mutans, salivarius, bovis, and pyogenes groups of streptococci...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27920580/positive-clinical-outcomes-derived-from-using-streptococcus-salivarius-k12-to-prevent-streptococcal-pharyngotonsillitis-in-children-a-pilot-investigation
#15
Francesco Di Pierro, Maria Colombo, Alberto Zanvit, Amilcare S Rottoli
BACKGROUND: Streptococcus salivarius K12 (BLIS K12(®)) is a probiotic strain producing the bacteriocins salivaricin A2 and salivaricin B, both of which strongly antagonize the growth of Streptococcus pyogenes, the most important bacterial cause of pharyngeal infections in humans. It successfully colonizes and exhibits persistence in the oral cavity and is endowed with an excellent safety profile. Previous observations of a small group of children indicated that the use of BLIS K12 could also reduce the occurrence of viral pharyngitis...
2016: Drug, Healthcare and Patient Safety
https://www.readbyqxmd.com/read/27902365/oral-cavities-of-healthy-infants-harbour-high-proportions-of-streptococcus-salivarius-strains-with-phenotypic-and-genotypic-resistance-to-multiple-classes-of-antibiotics
#16
Thaís H Palma, Erika N Harth-Chú, Jodie Scott, Rafael N Stipp, Heike Boisvert, Mariana F Salomão, Jéssica D Theobaldo, Rosana F Possobon, Leandro C Nascimento, Jonathan W McCafferty, Lina Faller, Margaret J Duncan, Renata O Mattos-Graner
Emerging antibiotic resistance in the oropharyngeal microbiota, of which Streptococcus salivarius is a prominent species, represents a challenge for treating paediatric populations. In this study, we investigated the role of Streptococcussalivarius as a reservoir for antibiotic resistance genes (ARG) in the oral microbiota by analysing 95 Streptococcussalivarius isolates from 22 healthy infants (2-16 months of age). MICs of penicillin G, amoxicillin, erythromycin, tetracycline, doxycycline and streptomycin were determined...
December 2016: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/27874935/effect-of-administration-of-streptococcus-salivarius-k12-on-the-occurrence-of-streptococcal-pharyngo-tonsillitis-scarlet-fever-and-acute-otitis-media-in-3-years-old-children
#17
F Di Pierro, M Colombo, M G Giuliani, M L Danza, I Basile, T Bollani, A M Conti, A Zanvit, A S Rottoli
OBJECTIVE: Streptococcus salivarius K12 (BLIS K12) is a probiotic strain strongly antagonistic to the growth of Streptococcus pyogenes, the most important bacterial cause of pharyngeal infections in humans. Shown to colonize the oral cavity and to be safe for human use, BLIS K12 has previously been reported to reduce pharyngo-tonsillitis episodes in children or adults known to have experienced recurrent streptococcal infection. The present study was focussed upon evaluating the role of BLIS K12 in the control of streptococcal disease and acute otitis media in children attending the first year of kindergarten...
November 2016: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/27812181/altered-gut-microbiota-composition-associated-with-eczema-in-infants
#18
Huajun Zheng, Hong Liang, Yuezhu Wang, Maohua Miao, Tao Shi, Fen Yang, Enuo Liu, Wei Yuan, Zai-Si Ji, De-Kun Li
Eczema is frequently the first manifestation of an atopic diathesis and alteration in the diversity of gut microbiota has been reported in infants with eczema. To identify specific bacterial communities associated with eczema, we conducted a case-control study of 50 infants with eczema (cases) and 51 healthy infants (controls). We performed high-throughput sequencing for V3-V4 hypervariable regions of the 16S rRNA genes from the gut fecal material. A total of 12,386 OTUs (operational taxonomic units) at a 97% similarity level were obtained from the two groups, and we observed a difference in taxa abundance, but not the taxonomic composition, of gut microbiota between the two groups...
2016: PloS One
https://www.readbyqxmd.com/read/27741258/candida-albicans-carriage-in-children-with-severe-early-childhood-caries-s-ecc-and-maternal-relatedness
#19
Jin Xiao, Yonghwi Moon, Lihua Li, Elena Rustchenko, Hironao Wakabayashi, Xiaoyi Zhao, Changyong Feng, Steven R Gill, Sean McLaren, Hans Malmstrom, Yanfang Ren, Robert Quivey, Hyun Koo, Dorota T Kopycka-Kedzierawski
INTRODUCTION: Candida albicans has been detected together with Streptococcus mutans in high numbers in plaque-biofilm from children with early childhood caries (ECC). The goal of this study was to examine the C. albicans carriage in children with severe early childhood caries (S-ECC) and the maternal relatedness. METHODS: Subjects in this pilot cross-sectional study were recruited based on a convenient sample. DMFT(S)/dmft(s) caries and plaque scores were assessed during a comprehensive oral exam...
2016: PloS One
https://www.readbyqxmd.com/read/27723640/antimicrobial-efficacy-of-the-combinations-of-acacia-nilotica-murrayakoenigii-linn-sprengel-eucalyptus-and-psidiumguajava-on-primary-plaque-colonizers-an-in-vitro-study
#20
Byalakere Rudraiah Chandra Shekar, Ramesh Nagarajappa, Rupal Singh, S Suma, Rupesh Thakur
BACKGROUND: The rise in disease incidence, increased resistance of pathogenic bacteria to currently used antibiotics and chemotherapeutics, opportunistic infections in immunocompromised individuals, and financial considerations in developing countries necessitates alternate preventive and treatment strategies for oral diseases. OBJECTIVE: The objective of the study is to assess the antimicrobial efficacy of triple and quadruple combinations of Acacia nilotica (AN), Murraya koenigii (Linn...
July 2016: Indian Journal of Dental Research: Official Publication of Indian Society for Dental Research
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