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Streptococcus salivarius

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https://www.readbyqxmd.com/read/28432093/diversity-of-integrative-and-conjugative-elements-of-streptococcus-salivarius-and-their-intra-and-interspecies-transfer
#1
Narimane Dahmane, Virginie Libante, Florence Charron-Bourgoin, Eric Guédon, Gérard Guédon, Nathalie Leblond-Bourget, Sophie Payot
Integrative and Conjugative Elements (ICEs) are widespread chromosomal mobile genetic elements, which can transfer autonomously by conjugation in bacteria. Thirteen ICEs with a conjugation module closely related to that of ICESt3 of Streptococcus thermophilus were characterized in Streptococcus salivarius by whole genome sequencing. Sequence comparison highlighted ICE evolution by shuffling of 3 different integration/excision modules (for integration in the 3' end of fda, rpsI or rpmG genes) with the conjugation module of the ICESt3 subfamily...
April 21, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28410499/comparing-the-impact-of-ultrafine-particles-from-petrodiesel-and-biodiesel-combustion-to-bacterial-metabolism-by-targeted-hplc-ms-ms-metabolic-profiling
#2
Fanyi Zhong, Mengyang Xu, Katie Schelli, Joshua Rutowski, Britt A Holmén, Jiangjiang Zhu
Alterations of gut bacterial metabolism play an important role in their host metabolism, and can result in diseases such as obesity and diabetes. While many factors were discovered influencing the gut bacterial metabolism, exposure to ultrafine particles (UFPs) from engine combustions were recently proposed to be a potential risk factor for the perturbation of gut bacterial metabolism, and consequentially to obesity and diabetes development. This study focused on evaluation of how UFPs from diesel engine combustions impact gut bacterial metabolism...
April 11, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28393073/a-comparative-study-of-oral-microbiota-in-infants-with-complete-cleft-lip-and-palate-or-cleft-soft-palate
#3
Agnieszka Machorowska-Pieniążek, Anna Mertas, Małgorzata Skucha-Nowak, Marta Tanasiewicz, Tadeusz Morawiec
Few reports have been published on the early microbiota in infants with various types of cleft palate. We assessed the formation of the oral microbiota in infants with complete cleft lip and palate (CLP n = 30) or cleft soft palate (CSP n = 25) in the neonatal period (T1 time) and again in the gum pad stage (T2 time). Culture swabs from the tongue, palate, and/or cleft margin at T1 and T2 were taken. We analysed the prevalence of the given bacterial species (the percentage) and the proportions in which the palate and tongue were colonised by each microorganism...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28384969/a-comparison-of-antibacterial-efficacy-of-0-5-sodium-fluoride-impregnated-miswak-and-plain-miswak-sticks-on-streprococcus-mutans-a-randomized-controlled-trial
#4
Romshi Raina, Vinod Kumar, Madhusudan Krishna, Sakshi Raina, Ashish Jaiswal, Arul Selvan, Chaitra Patil, Sneh Kalgotra
INTRODUCTION: Miswak (Salvadora persica) is a tooth cleaning stick made from the Salvodora Persica tree. It serves as a natural dentifrice with anti-bacterial, anti-plaque and anti-fungal properties. Fluoride, impregnated in chewing sticks will bestow comparable safety against dental caries as the fluoride containing dentifrice. AIM: The aim of the present study was to assess and compare the anti-bacterial effect of 0.5% sodium fluoride impregnated miswak and plain miswak sticks on Streptococcus mutans...
February 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28374172/evaluation-of-potential-probiotics-isolated-from-human-milk-and-colostrum
#5
Quésia S Damaceno, Jaqueline P Souza, Jacques R Nicoli, Raquel L Paula, Gabriela B Assis, Henrique C Figueiredo, Vasco Azevedo, Flaviano S Martins
Several studies have demonstrated a diversity of bacterial species in human milk, even in aseptically collected samples. The present study evaluated potential probiotic bacteria isolated from human milk and associated maternal variables. Milk samples were collected from 47 healthy women and cultured on selective and universal agar media under aerobic and anaerobic conditions. Bacterial isolates were counted and identified by Biotyper Matrix-Assisted Laser Desorption Ionization-Time of Flight mass spectrometry and then tested for probiotic properties...
April 3, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28328023/identification-of-microorganisms-in-biofluids-of-individuals-with-periodontitis-and-chronic-kidney-disease-using-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry
#6
Levy Anderson César Alves, Rafael Celestino Souza, Taciana Mara Couto da Silva, Andreia Watanabe, Meriellen Dias, Maria Anita Mendes, Ana Lídia Ciamponi
RATIONALE: Chronic kidney disease (CKD) and periodontitis (PD) are important health issues. There is a large variety of microorganisms related to the pathogenesis of periodontitis, and optimising the time and the cost of laboratory assays to detect these organisms is highly valuable in the medical field. METHODS: Bacteria were isolated from saliva and oral biofilm of 30 adolescents and young adults with definite medical and dental diagnosis of CKD and PD, respectively, and proteins were extracted for microorganism identification by means of the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) technique...
May 30, 2016: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/28275789/-preparation-and-identification-of-polyclonal-antibody-of-streptococcus-salivarius-57-i-urease
#7
Yan Wang, Dan-Ying Tao, Xi-Ping Feng
PURPOSE: To prepare and identify polyclonal antibody of Streptococcus salivarius 57.I urease. METHODS: The biggest structural subunit of Streptococcus salivarius 57.I urease, UreC, was obtained by gene clone, IPTG-induced expression, and purification through affinity chromatography. Anti-sera and polyclonal antibody were raised by immunizing rabbits with purified UreC. Western blot was utilized to detect the specific combination of polyclonal antibody with UreC...
December 2016: Shanghai Kou Qiang Yi Xue, Shanghai Journal of Stomatology
https://www.readbyqxmd.com/read/28274037/antimicrobial-effects-of-garcinia-mangostana-on-cariogenic-microorganisms
#8
Sunitha Janardhanan, Jaideep Mahendra, A S Smiline Girija, Little Mahendra, Vijayashree Priyadharsini
INTRODUCTION: Garcinia mangostana commonly called as Mangosteen fruit has been used as an antibacterial agent since age old times. The mangosteen pericarp has proven to have antibacterial effect, but the effect of the same on cariogenic organisms has not been explored. The present study was an attempt to gain a better understanding of the antibacterial effect of mangosteen pericarp on the cariogenic bacteria, to unravel the therapeutic potential for the same. AIM: The aim of the study was to assess the antibacterial efficacy of the crude chloroform extract of mangosteen pericarp against cariogenic bacteria...
January 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28270703/effectiveness-of-salvadora-persica-extracts-against-common-oral-pathogens
#9
Hanan Balto, Ibrahim Al-Sanie, Sultan Al-Beshri, Abdullah Aldrees
OBJECTIVE: The purpose of this study was to evaluate the antibacterial activity of ethanol and hexane extracts of Salvadora persica against common oral pathogens. MATERIALS AND METHODS: Well diffusion, Minimum Inhibition Concentration (MIC), Minimum Bactericidal Concentration (MBC), and Broth microdilution tests were used to determine the optimum antimicrobial concentrations of S. persica extracts against Streptococcus mutans (S. mutans), Streptococcus sanguis (S...
January 2017: Saudi Dental Journal
https://www.readbyqxmd.com/read/28259058/community-analysis-of-dental-plaque-and-endotracheal-tube-biofilms-from-mechanically-ventilated-patients
#10
Poala J Marino, Matt P Wise, Ann Smith, Julian R Marchesi, Marcello P Riggio, Michael A O Lewis, David W Williams
PURPOSE: Mechanically ventilated patients are at risk for developing ventilator-associated pneumonia, and it has been reported that dental plaque provides a reservoir of respiratory pathogens that may aspirate to the lungs and endotracheal tube (ETT) biofilms. For the first time, metataxonomics was used to simultaneously characterize the microbiome of dental plaque, ETTs, and non-directed bronchial lavages (NBLs) in mechanically ventilated patients to determine similarities in respective microbial communities and therefore likely associations...
June 2017: Journal of Critical Care
https://www.readbyqxmd.com/read/28253182/effectiveness-of-casein-phosphopeptide-amorphous-calcium-phosphate-and-lysozyme-lactoferrin-and-lactoperoxidase-in-reducing-streptococcus-mutans-counts-in-dentinal-caries
#11
Sérgio Luiz Pinheiro, Giuliana Rodrigues Azenha, Giovana Spagnolo Albamonte Araujo, Regina Maria Puppin Rontani
This study compared the capacity of casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) to that of a combination of lysozyme, lactoferrin, and lactoperoxidase (LLL) in root canal disinfectant for reducing the Streptococcus mutans counts from dentinal caries. Forty human permanent third molars were selected, and flat dentin surfaces were created. Carious lesions were induced using a microbiological model. The specimens were randomly divided into 2 groups (n = 20) according to the type of agent used: group 1, CPP-ACP; group 2, LLL...
March 2017: General Dentistry
https://www.readbyqxmd.com/read/28236205/influence-of-oral-probiotic-streptococcus-salivarius-k12-on-ear-and-oral-cavity-health-in-humans-systematic-review
#12
Katarina Zupancic, Valentina Kriksic, Irena Kovacevic, Dujo Kovacevic
Traditionally, probiotics are linked to the good health of the intestine and most clinical studies focus on that field. Evidence of oral probiotic use for ear and oral cavity disease prevention with impact on human health is limited. This work reviews existing studies and literature on Streptococcus salivarius K12 as an oral probiotic and effects of S. salivarius K12 on human ear and oral cavity human health. The studies were accessed via database searches: MEDLINE, PubMed, and Elsevier. The search included/focused on/encompassed publications from 2003 to 2016 with keywords related to K12 Streptococcus salivarius, bacteriocin-like inhibitory substances (BLIS) K12, probiotic K12 salivarius, and K12 probiotic health effects...
February 24, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28194750/differential-responses-of-human-dendritic-cells-to-metabolites-from-the-oral-airway-microbiome
#13
K Whiteson, S Agrawal, A Agrawal
Small molecule metabolites that are produced or altered by host-associated microbial communities are emerging as significant immune response modifiers. However, there is a key gap in our knowledge of how oral microbial metabolites affect the immune response. Here, we examined the effects of metabolites from five bacterial strains found commonly in the oral/airway microbial communities of humans. The five strains, each isolated from cystic fibrosis patient sputum, were Pseudomonas aeruginosa FLR01 non-mucoid (P1) and FLR02 mucoid (P2) forms, Streptococcus pneumoniae (Sp), S...
February 14, 2017: Clinical and Experimental Immunology
https://www.readbyqxmd.com/read/28187188/development-of-a-flow-chamber-system-for-the-reproducible-in-vitro-analysis-of-biofilm-formation-on-implant-materials
#14
Henryke Rath, Sascha Nico Stumpp, Meike Stiesch
Since the introduction of modern dental implants in the 1980s, the number of inserted implants has steadily increased. Implant systems have become more sophisticated and have enormously enhanced patients' quality of life. Although there has been tremendous development in implant materials and clinical methods, bacterial infections are still one of the major causes of implant failure. These infections involve the formation of sessile microbial communities, called biofilms. Biofilms possess unique physical and biochemical properties and are hard to treat conventionally...
2017: PloS One
https://www.readbyqxmd.com/read/28165440/antimicrobial-efficacy-of-probiotic-containing-toothpastes-an-in-vitro-evaluation
#15
Ivana Parčina Amižić, Livia Cigić, Lidia Gavić, Marina Radić, Dolores Biočina Lukenda, Marija Tonkić, Ivana Goić Barišić
Aim To evaluate, in vitro antimicrobial ability of two probiotic toothpastes (one containing Lactobacillus paracasei, other containing Lactobacillus acidophilus) and one toothpaste without probiotic separately, and in a combination with two different mouthrinses (one containing essential oils and the other containing hexitidine). Methods Antimicrobial susceptibility was checked by using the ditch method and Clinical Laboratory Standard Institute (CLSI) guidelines. Two different toothpastes with probiotic, toothpaste without probiotic and two different mouthrinses were tested against the following selected microorganisms: Candida albicans, Candida tropicalis, Enterococcus faecalis, Streptococcus salivarius and Staphylococcus aureus...
February 1, 2017: Medicinski Glasnik
https://www.readbyqxmd.com/read/28144393/percutaneous-drainage-as-a-first-therapeutic-step-prior-to-surgery-in-liver-hydatid-cyst-abscess-is-it-worth-it
#16
Aylhin J Lopez-Marcano, Jose M Ramia, Vladimir Arteaga, Roberto De la Plaza, Jhonny D Gonzales, Anibal Medina
AIM: To delay surgery until the patient is in a better condition, and thus to decrease postoperative morbidity. METHODS: Using this algorithm we treated three patients aged 55, 75 and 80 years. In all three patients the clinical presentation was fever without a clear source of infection; all had nonspecific symptoms such as general malaise, dyspnea, and abdominal discomfort in the previous 15 d. They came to the emergency room at our hospital due to deterioration of their general condition...
January 18, 2017: World Journal of Hepatology
https://www.readbyqxmd.com/read/28139479/antibacterial-effect-and-physical-properties-of-chitosan-and-chlorhexidine-cetrimide-modified-glass-ionomer-cements
#17
Apurva Mishra, Ramesh Kumar Pandey, Natesan Manickam
AIMS: To compare antibacterial effect and physical properties of chitosan (CH) modified glass ionomer cement (GIC) (10% v/v), chlorhexidine-cetrimide (CHX-CT) modified GIC (2.5/2.5% w/w) and conventional GIC. MATERIALS AND METHODS: A total of fifty healthy children of age 7-12 years were selected and randomly assigned to class A and B for in vivo analysis. Slabs of CH modified GIC (Group II) along with slabs of conventional GIC (Group I, control) were cemented on buccal surfaces of maxillary molars (split-mouth technique) for class A children...
January 2017: Journal of the Indian Society of Pedodontics and Preventive Dentistry
https://www.readbyqxmd.com/read/28135680/biofunctional-polyethylene-glycol-coatings-on-titanium-an-in-vitro-based-comparison-of-functionalization-methods
#18
Judit Buxadera-Palomero, Cristina Calvo, Sergi Torrent-Camarero, F Javier Gil, Carlos Mas-Moruno, Cristina Canal, Daniel Rodríguez
Three methods for the production of Polyethylene glycol (PEG) coatings on titanium are compared, i.e. plasma polymerization, electrodeposition and silanization. The compared deposition methods presented similar wettability (hydrophilic coatings), chemical composition assessed by XPS and thickness around 1nm. The coatings lowered albumin adsorption and presented a decreased fibroblast, Streptococcus sanguinis and Lactobacillus salivarius adhesion. Immobilization of a cell adhesion peptide (RGD) presented a higher fibroblast adhesion and no alteration of the bacterial adhesion, giving three methods for the biofunctionalization of titanium for dental implants...
April 1, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28127162/comparative-evaluation-of-0-2-chlorhexidine-mouthwash-xylitol-chewing-gum-and-combination-of-0-2-chlorhexidine-mouthwash-and-xylitol-chewing-gum-on-salivary-streptococcus-mutans-and-biofilm-levels-in-8-to-12-year-old-children
#19
Meena Syed, Radhika Chopra, Vandana Shrivastava, Vinod Sachdev
AIM: To assess the effect of combining 0.2% chlorhexidine (CHX) mouthwash with xylitol (XYL) chewing gum on Streptococcus mutans and biofilm levels among 8- to 12-year-old children. MATERIALS AND METHODS: Sixty children aged 8 to 12 years were selected with moderate and high salivary S. mutans levels. They were divided into three groups of 20 children each: (1) XYL group where the subjects chewed XYL twice daily; (2) CHX where rinsing was done twice daily; and (3) combination of XYL and CHX group (XYL+CHX) where both the agents were used once daily...
October 2016: International Journal of Clinical Pediatric Dentistry
https://www.readbyqxmd.com/read/28070022/stable-isotope-profiles-reveal-active-production-of-vocs-from-human-associated-microbes
#20
Joann Phan, Simone Meinardi, Barbara Barletta, Donald Blake, Katrine Whiteson
Volatile organic compounds (VOCs) measured from exhaled breath have great promise for the diagnosis of bacterial infections. However, determining human or microbial origin of VOCs detected in breath remains a great challenge. For example, the microbial fermentation product 2,3-butanedione was recently found in the breath of CF patients; parallel culture-independent metagenomic sequencing of the same samples revealed that Streptococcus and Rothia spp. have the genetic capacity to produce 2,3-butanedione. To investigate whether the genetic capacity found in metagenomes translates to bacterial production of a VOC of interest such as 2,3-butanedione, we fed stable isotopes to three bacterial strains isolated from Cystic Fibrosis (CF) patients: two Gram-positive bacteria, Rothia mucilaginosa and Streptococcus salivarius, and a dominant opportunistic Gram-negative pathogen, Pseudomonas aeruginosa...
January 10, 2017: Journal of Breath Research
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