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Streptococcus salivarius

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https://www.readbyqxmd.com/read/28070022/stable-isotope-profiles-reveal-active-production-of-vocs-from-human-associated-microbes
#1
Joann Phan, Simone Meinardi, Barbara Barletta, Donald Blake, Katrine Whiteson
Volatile organic compounds (VOCs) measured from exhaled breath have great promise for the diagnosis of bacterial infections. However, determining human or microbial origin of VOCs detected in breath remains a great challenge. For example, the microbial fermentation product 2,3-butanedione was recently found in the breath of CF patients; parallel culture-independent metagenomic sequencing of the same samples revealed that Streptococcus and Rothia spp. have the genetic capacity to produce 2,3-butanedione. To investigate whether the genetic capacity found in metagenomes translates to bacterial production of a VOC of interest such as 2,3-butanedione, we fed stable isotopes to three bacterial strains isolated from Cystic Fibrosis (CF) patients: two Gram-positive bacteria, Rothia mucilaginosa and Streptococcus salivarius, and a dominant opportunistic Gram-negative pathogen, Pseudomonas aeruginosa...
January 10, 2017: Journal of Breath Research
https://www.readbyqxmd.com/read/28062463/ymc-2011-a-temperate-phage-of-streptococcus-salivarius-57-i
#2
Wen-Chun Chou, Szu-Chuan Huang, Cheng-Hsun Chiu, Yi-Ywan M Chen
: Streptococcus salivarius is an abundant isolate of the oral cavity. The genome of S. salivarius 57.I consists of a 2-Mb chromosome and a 40,758-bp circular molecule, designated YMC-2011. Annotation of YMC-2011 revealed 55 open reading frames, most of them associated with phage production, although plaque formation is not observed in S. salivarius 57.I after lytic induction using mitomycin C. Results from Southern hybridization and quantitative real-time PCR confirmed that YMC-2011 exists extrachromosomally with an estimated copy number of 3 to 4...
January 6, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28029097/influence-of-silver-content-on-rifampicin-adsorptivity-for-magnetite-ag-rifampicin-nanoparticles
#3
Olena Ivashchenko, Emerson Coy, Barbara Peplinska, Marcin Jarek, Mikołaj Lewandowski, Karol Załęski, Alicja Warowicka, Anna Wozniak, Tatiana Babutina, Justyna Jurga-Stopa, Janez Dolinsek, Stefan Jurga
Magnetite nanoparticles (NPs) decorated with silver (magnetite/Ag) are intensively investigated due to their application in the biomedical field. We demonstrate that the increase of silver content on the surface of nanoparticles improves the adsorptivity of antibiotic rifampicin as well as antibacterial properties. The use of ginger extract allowed to improve the silver nucleation on the magnetite surface that resulted in an increase of silver content. Physicochemical and functional characterization of magnetite/Ag NPs was performed...
February 3, 2017: Nanotechnology
https://www.readbyqxmd.com/read/27999400/comparative-study-on-the-characteristics-of-weissella-cibaria-cmu-and-probiotic-strains-for-oral-care
#4
Hye-Jin Jang, Mi-Sun Kang, Sung-Hun Yi, Ji-Young Hong, Sang-Pil Hong
Probiotics have been demonstrated as a new paradigm to substitute antibiotic treatment for dental caries, gingivitis, and chronic periodontitis. The present work was conducted to compare the characteristics of oral care probiotics: Weissella cibaria CMU (Chonnam Medical University) and four commercial probiotic strains. Survival rates under poor oral conditions, acid production, hydrogen peroxide production, as well as inhibition of biofilm formation, coaggregation, antibacterial activity, and inhibition of volatile sulfur compounds were evaluated...
December 20, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27994423/assessment-of-streptococcus-mutans-in-healthy-versus-gingivitis-and-chronic-periodontitis-a-clinico-microbiological-study
#5
Sneha Dani, Ashwin Prabhu, K R Chaitra, N C Desai, Sudhir R Patil, Ranjan Rajeev
BACKGROUND: Dental caries and periodontal disease are most common oral diseases. Streptococcus mutans are considered to be the major pathogens in initiation of dental caries. Evidence shows that periodontal disease and caries share a number of contributory factors. Thus in view of these findings it would be worthwhile to examine whether Streptococcus mutans persist within the saliva and subgingival environment of the periodontitis patients and to determine whether there is any association between Streptococcus mutans colonization, pH of saliva and sub-gingival plaque pH in periodontal diseases before therapy...
October 2016: Contemporary Clinical Dentistry
https://www.readbyqxmd.com/read/27932242/elevated-salivary-iga-decreased-anxiety-and-an-altered-oral-microbiota-are-associated-with-active-participation-on-an-undergraduate-athletic-team
#6
Ashley L Lamb, Debra E Hess, Sherie Edenborn, Elizabeth Ubinger, Andres E Carrillo, Pierette M Appasamy
BACKGROUND: Previous reports indicate that regular, but not excessive, exercise can moderate the response to anxiety and alter the immune response, therefore we hypothesized that college student athletes who were actively participating on an NCAA Division III athletics team ("in-season") would have lower levels of anxiety and higher salivary IgA levels than similar college athletes who were in their "off-season". NCAA Division III athletes participate in athletics at a level of intensity that is more moderate compared to other NCAA divisions...
February 1, 2017: Physiology & Behavior
https://www.readbyqxmd.com/read/27924597/natural-transformation-of-oral-streptococci-by-use-of-synthetic-pheromones
#7
Gabriela Salvadori, Roger Junges, Rabia Khan, Heidi A Åmdal, Donald A Morrison, Fernanda C Petersen
The discovery that Streptococcus pneumoniae uses a competence-stimulating peptide (CSP) to induce competence for natural transformation, and that other species of the mitis and the anginosus streptococcal groups use a similar system, has expanded the tools to explore gene function and regulatory pathways in streptococci. Two other classes of pheromones have been discovered since then, comprising the bacteriocin-inducing peptide class found in Streptococcus mutans (also named CSP, although different from the former) and the SigX-inducing peptides (XIP), in the mutans, salivarius, bovis, and pyogenes groups of streptococci...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27920580/positive-clinical-outcomes-derived-from-using-streptococcus-salivarius-k12-to-prevent-streptococcal-pharyngotonsillitis-in-children-a-pilot-investigation
#8
Francesco Di Pierro, Maria Colombo, Alberto Zanvit, Amilcare S Rottoli
BACKGROUND: Streptococcus salivarius K12 (BLIS K12(®)) is a probiotic strain producing the bacteriocins salivaricin A2 and salivaricin B, both of which strongly antagonize the growth of Streptococcus pyogenes, the most important bacterial cause of pharyngeal infections in humans. It successfully colonizes and exhibits persistence in the oral cavity and is endowed with an excellent safety profile. Previous observations of a small group of children indicated that the use of BLIS K12 could also reduce the occurrence of viral pharyngitis...
2016: Drug, Healthcare and Patient Safety
https://www.readbyqxmd.com/read/27902365/oral-cavities-of-healthy-infants-harbour-high-proportions-of-streptococcus-salivarius-strains-with-phenotypic-and-genotypic-resistance-to-multiple-classes-of-antibiotics
#9
Thaís H Palma, Erika N Harth-Chú, Jodie Scott, Rafael N Stipp, Heike Boisvert, Mariana F Salomão, Jéssica D Theobaldo, Rosana F Possobon, Leandro C Nascimento, Jonathan W McCafferty, Lina Faller, Margaret J Duncan, Renata O Mattos-Graner
Emerging antibiotic resistance in the oropharyngeal microbiota, of which Streptococcus salivarius is a prominent species, represents a challenge for treating paediatric populations. In this study, we investigated the role of Streptococcussalivarius as a reservoir for antibiotic resistance genes (ARG) in the oral microbiota by analysing 95 Streptococcussalivarius isolates from 22 healthy infants (2-16 months of age). MICs of penicillin G, amoxicillin, erythromycin, tetracycline, doxycycline and streptomycin were determined...
December 2016: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/27874935/effect-of-administration-of-streptococcus-salivarius-k12-on-the-occurrence-of-streptococcal-pharyngo-tonsillitis-scarlet-fever-and-acute-otitis-media-in-3-years-old-children
#10
F Di Pierro, M Colombo, M G Giuliani, M L Danza, I Basile, T Bollani, A M Conti, A Zanvit, A S Rottoli
OBJECTIVE: Streptococcus salivarius K12 (BLIS K12) is a probiotic strain strongly antagonistic to the growth of Streptococcus pyogenes, the most important bacterial cause of pharyngeal infections in humans. Shown to colonize the oral cavity and to be safe for human use, BLIS K12 has previously been reported to reduce pharyngo-tonsillitis episodes in children or adults known to have experienced recurrent streptococcal infection. The present study was focussed upon evaluating the role of BLIS K12 in the control of streptococcal disease and acute otitis media in children attending the first year of kindergarten...
November 2016: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/27812181/altered-gut-microbiota-composition-associated-with-eczema-in-infants
#11
Huajun Zheng, Hong Liang, Yuezhu Wang, Maohua Miao, Tao Shi, Fen Yang, Enuo Liu, Wei Yuan, Zai-Si Ji, De-Kun Li
Eczema is frequently the first manifestation of an atopic diathesis and alteration in the diversity of gut microbiota has been reported in infants with eczema. To identify specific bacterial communities associated with eczema, we conducted a case-control study of 50 infants with eczema (cases) and 51 healthy infants (controls). We performed high-throughput sequencing for V3-V4 hypervariable regions of the 16S rRNA genes from the gut fecal material. A total of 12,386 OTUs (operational taxonomic units) at a 97% similarity level were obtained from the two groups, and we observed a difference in taxa abundance, but not the taxonomic composition, of gut microbiota between the two groups...
2016: PloS One
https://www.readbyqxmd.com/read/27741258/candida-albicans-carriage-in-children-with-severe-early-childhood-caries-s-ecc-and-maternal-relatedness
#12
Jin Xiao, Yonghwi Moon, Lihua Li, Elena Rustchenko, Hironao Wakabayashi, Xiaoyi Zhao, Changyong Feng, Steven R Gill, Sean McLaren, Hans Malmstrom, Yanfang Ren, Robert Quivey, Hyun Koo, Dorota T Kopycka-Kedzierawski
INTRODUCTION: Candida albicans has been detected together with Streptococcus mutans in high numbers in plaque-biofilm from children with early childhood caries (ECC). The goal of this study was to examine the C. albicans carriage in children with severe early childhood caries (S-ECC) and the maternal relatedness. METHODS: Subjects in this pilot cross-sectional study were recruited based on a convenient sample. DMFT(S)/dmft(s) caries and plaque scores were assessed during a comprehensive oral exam...
2016: PloS One
https://www.readbyqxmd.com/read/27723640/antimicrobial-efficacy-of-the-combinations-of-acacia-nilotica-murrayakoenigii-linn-sprengel-eucalyptus-and-psidiumguajava-on-primary-plaque-colonizers-an-in-vitro-study
#13
Byalakere Rudraiah Chandra Shekar, Ramesh Nagarajappa, Rupal Singh, S Suma, Rupesh Thakur
BACKGROUND: The rise in disease incidence, increased resistance of pathogenic bacteria to currently used antibiotics and chemotherapeutics, opportunistic infections in immunocompromised individuals, and financial considerations in developing countries necessitates alternate preventive and treatment strategies for oral diseases. OBJECTIVE: The objective of the study is to assess the antimicrobial efficacy of triple and quadruple combinations of Acacia nilotica (AN), Murraya koenigii (Linn...
July 2016: Indian Journal of Dental Research: Official Publication of Indian Society for Dental Research
https://www.readbyqxmd.com/read/27681377/investigation-of-streptococcus-salivarius-mediated-inhibition-of-pneumococcal-adherence-to-pharyngeal-epithelial-cells
#14
Jayne Manning, Eileen M Dunne, Philip A Wescombe, John D F Hale, E Kim Mulholland, John R Tagg, Roy M Robins-Browne, Catherine Satzke
BACKGROUND: Pneumococcal adherence to the nasopharyngeal epithelium is a critical step in colonisation and disease. The probiotic bacterium, Streptococcus salivarius, can inhibit pneumococcal adherence to epithelial cells in vitro. We investigated the mechanism(s) of inhibition using a human pharyngeal epithelial cell line (Detroit 562) following pre-administration of two different strains of S. salivarius. RESULTS: Whilst the bacteriocin-encoding megaplasmids of S...
September 29, 2016: BMC Microbiology
https://www.readbyqxmd.com/read/27668898/antimicrobial-susceptibility-against-penicillin-ampicillin-and-vancomycin-of-viridans-group-streptococcus-in-oral-microbiota-of-patients-at-risk-of-infective-endocarditis
#15
Serap Süzük, Banu Kaşkatepe, Mustafa Çetin
The viridans group Streptococci (VGS) are most abundant in the mouth; in some instances they might emerge as pathogens particularly in infective endocarditis (IE). In this study, we aimed to define and determine the susceptibility against antibiotics of VGS that are members of the oral microbiota of patients exhibiting a risk of developing IE. Forty-nine patients at risk of infective endocarditis were included in the study. Identification of the bacteria was performed using API STREP (bioMérieux, France). Gradient test strips (E-Test, France) were used to determine MIC of the bacteria against penicillin, ampicillin, and vancomycin...
September 1, 2016: Le Infezioni in Medicina
https://www.readbyqxmd.com/read/27660775/genome-sequence-of-the-oral-probiotic-streptococcus-salivarius-jf
#16
Fang Jia
Streptococcus salivarius is a nonpathogenic Gram-positive bacterium and the predominant colonizer of the oral microbiota. It finds a wide application in the prevention of upper respiratory tract infections, also reducing the frequency of other main pathogens. Here, we present the complete genome sequence of the oral probiotic S. salivarius JF.
2016: Genome Announcements
https://www.readbyqxmd.com/read/27570236/identification-of-vaginal-fluid-saliva-and-feces-using-microbial-signatures-in-a-han-chinese-population
#17
Kai-Nan Zou, Li-Jie Ren, Yuan Ping, Ke Ma, Hui Li, Yu Cao, Huai-Gu Zhou, Yi-Liang Wei
In recent years, forensic scientists have focused on the discrimination of body fluids using microbial signatures. In this study, we performed PCR-based detection of microbial signatures of vaginal fluid, saliva, and feces in a Han Chinese population. We investigated the 16S rRNA genes of Lactobacillus crispatus, Lactobacillus gasseri, Lactobacillus jensenii, Lactobacillus iners, and Atopobium vaginae in vaginal fluid, the 16S rRNA and the glucosyltransferase enzyme genes of Streptococcus salivarius and Streptococcus mutans in saliva, and the 16S rRNA genes of Enterococcus species, the RNA polymerase β-subunit gene of Bacteroides uniformis and Bacteroides vulgatus, and the α-1-6 mannanase gene of Bacteroides thetaiotaomicron in feces...
October 2016: Journal of Forensic and Legal Medicine
https://www.readbyqxmd.com/read/27569711/oral-microflora-and-selection-of-resistance-after-a-single-dose-of%C3%A2-amoxicillin
#18
D Khalil, M Hultin, M U Rashid, B Lund
The study aimed to determine the effects of a single-dose antibiotic prophylaxis on normal oral microflora. A single dose of 2 g amoxicillin was given to 29 healthy volunteers. Saliva was collected before antibiotic administration (day 1), and again on days 2, 5, 10, 17 and 24 and subjected to culturing and antibiotic sensitivity analysis. Twenty-one per cent (6/29) of the individuals carried penicillin-V- and amoxicillin-resistant viridans streptococci before antibiotic administration. After a single dose of amoxicillin there was a significant reduction in Streptococcus salivarius on days 2 and 5, a significant reduction in other viridans streptococci on day 2 and the proportion of viridans streptococci with reduced susceptibility to amoxicillin was significantly increased on days 2 and 5...
November 2016: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/27557918/effect-of-tooth-mousse-on-streptococcus-mutans-in-the-plaque-of-high-caries-risk-preschool-children-a-triple-blind-randomized-clinical-trial
#19
Amira N Fadl, Magda M ElTekeya, Karin L Dowidar, Nadia Mokhles, Maha M El Tantawi
PURPOSE: Control of Streptococcus mutans can prevent caries in high-risk preschool children. The purpose of this study was to assess the effect of casein phosphopeptide amorphous calcium phosphate (CPP-ACP) on Streptococcus mutans in the plaque of high-risk preschool children. METHODS: A triple blind, randomized, controlled clinical trial included 40 healthy three- to five-year-olds with at least one white spot lesion. They were randomized into test (daily applications of GC Tooth Mousse:CPP-ACP) and control groups (placebo mousse)...
2016: Pediatric Dentistry
https://www.readbyqxmd.com/read/27538256/cytotoxicity-and-the-effect-of-cationic-peptide-fragments-against-cariogenic-bacteria-under-planktonic-and-biofilm-conditions
#20
Paula Fernanda Kreling, Kelly Limi Aida, Loiane Massunari, Karina Sampaio Caiaffa, Célio Percinoto, Telma Blanca Lombardo Bedran, Denise Madalena Palomari Spolidorio, Gabriel Flores Abuna, Eduardo Maffud Cilli, Cristiane Duque
This study evaluated the cytotoxicity and effect of fragments derived from three oral cationic peptides (CP): LL-37, D6-17 and D1-23 against cariogenic bacteria under planktonic and biofilm conditions. For cytotoxicity analysis, two epithelial cell lines were used. The minimum inhibitory concentration and the minimal bactericidal concentration were determined for the CP fragments and the control (chlorhexidine-CHX) against cariogenic bacteria. The fractional inhibitory concentration was obtained for the combinations of CP fragments on Streptococcus mutans...
October 2016: Biofouling
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