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Streptococcus salivarius

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https://www.readbyqxmd.com/read/29792173/impact-of-growth-ph-and-glucose-concentrations-on-the-cody-regulatory-network-in-streptococcus-salivarius
#1
Jianing Geng, Szu-Chuan Huang, Yueh-Ying Chen, Cheng-Hsun Chiu, Songnian Hu, Yi-Ywan M Chen
BACKGROUND: Streptococcus salivarius is an abundant isolate of the human oral microbiota. Since both pH and glucose availability fluctuate frequently in the oral cavity, the goal of this study was to investigate regulation by CodY, a conserved pleiotropic regulator of Gram positive bacteria, in response to these two signals. The chemostat culture system was employed to precisely control the growth parameters, and the transcriptomes of wild-type S. salivarius 57.I and its CodY-null derivative (ΔcodY) grown at pH 7 and 5...
May 23, 2018: BMC Genomics
https://www.readbyqxmd.com/read/29786169/bangladeshi-children-with-acute-diarrhea-show-fecal-microbiomes-with-increased-streptococcus-abundance-irrespective-of-diarrhea-etiology
#2
Silas Kieser, Shafiqul A Sarker, Olga Sakwinska, Francis Foata, Shamima Sultana, Zeenat Khan, Shoheb Islam, Nadine Porta, Séverine Combremont, Bertrand Betrisey, Coralie Fournier, Aline Charpagne, Patrick Descombes, Annick Mercenier, Bernard Berger, Harald Brüssow
We report streptococcal dysbiosis in acute diarrhea irrespective of etiology. Compared to 20 healthy local controls, 71 Bangladeshi children hospitalized with acute diarrhea (AD) of viral, mixed viral/bacterial, bacterial and unknown etiology showed a significantly decreased bacterial diversity with loss of pathways characteristic for the healthy distal colon microbiome (mannan degradation, methylerythritol phosphate and thiamin biosynthesis), an increased proportion of fecal streptococci belonging to the Streptococcus bovis and Streptococcus salivarius species complexes, and an increased level of E...
May 22, 2018: Environmental Microbiology
https://www.readbyqxmd.com/read/29776416/rapid-urease-test-rut-for-evaluation-of-urease-activity-in-oral-bacteria-in-vitro-and-in-supragingival-dental-plaque-ex-vivo
#3
Gunnar Dahlén, Haidar Hassan, Susanne Blomqvist, Anette Carlén
BACKGROUND: Urease is an enzyme produced by plaque bacteria hydrolysing urea from saliva and gingival exudate into ammonia in order to regulate the pH in the dental biofilm. The aim of this study was to assess the urease activity among oral bacterial species by using the rapid urease test (RUT) in a micro-plate format and to examine whether this test could be used for measuring the urease activity in site-specific supragingival dental plaque samples ex vivo. METHODS: The RUT test is based on 2% urea in peptone broth solution and with phenol red at pH 6...
May 18, 2018: BMC Oral Health
https://www.readbyqxmd.com/read/29764202/comparative-study-of-the-synbiotic-effect-of-inulin-and-fructooligosaccharide-with-probiotics-with-regard-to-the-various-properties-of-fermented-soy-milk
#4
Shalini Mishra, H N Mishra
Numerous combinations of probiotics were explored to find the suitable starter culture for the development of synbiotic soy yoghurt which can give good product characteristics and may be acceptable among consumers. Prebiotics (fructooligosaccharide (FOS) and inulin) were supplemented in an attempt to reduce the after-taste of soymilk, improve acidification profile and growth of probiotics. The addition of prebiotics in soy milk significantly enhanced the acidification rate (10.82 to 23.00 × 10-3 pH units/min) and condensed the fermentation completion time...
January 1, 2018: Food Science and Technology International, Ciencia y Tecnología de Los Alimentos Internacional
https://www.readbyqxmd.com/read/29755627/molecular-diagnosis-of-bacterial-definite-infective-endocarditis-by-real-time-polymerase-chain-reaction
#5
Reza Faraji, Mostafa Behjati-Ardakani, Nooshin Faraji, Seyed Mohammad Moshtaghioun, Seyed Mehdi Kalantar, Ali Pedarzadeh, Hengameh Zandi, Mohammadtaghi Sarebanhassanabadi, Nastaran Ahmadi, Ali Dehghani Firoozabadi
Infective endocarditis (IE) can be diagnosed using the Duke criteria, which cannot be conclusive especially when the results of blood cultures are negative. This study aimed at using real-time polymerase chain reaction (PCR) technique to isolate bacteria present in whole blood samples of patients with definitive IE on the basis of the method designed in this study. This laboratory and test study was conducted on 20 whole blood samples taken from patients with definitive IE. Real-time PCR of the 16s rRNA was utilized to directly analyze whole blood samples to diagnose bacterial IE...
April 2018: Cardiology Research
https://www.readbyqxmd.com/read/29725229/some-properties-of-fresh-and-ripened-traditional-akcakatik-cheese
#6
Şimşek Bedia, Tuncer Yasin
Akcakatik cheese (yogurt cheese) is produced by drying strained yogurt with or without adding cloves or black cumin. The main objective of this study was to detect the properties of both fresh and ripened Akcakatik cheeses and to compare them. For this purpose the biogenic amine content, volatile flavor compounds, protein degradation level, chemical properties and some microbiological properties of 15 Akcakatik cheese samples were investigated. Titratable acidity, total dry matter, NaCl, total nitrogen, water soluble nitrogen, ripened index, histamine, diacetyl and acetaldehyde levels were found to be higher in ripened cheese samples than in fresh cheese samples...
February 2018: Korean Journal for Food Science of Animal Resources
https://www.readbyqxmd.com/read/29611435/chemical-composition-and-in-vitro-antibacterial-and-antiproliferative-activities-of-the-essential-oil-from-the-leaves-of-psidium-myrtoides-o-berg-myrtaceae
#7
Alline L B Dias, Hellen R F Batista, Elisângela B B Estevam, Cassia C F Alves, Moacir R Forim, Heloiza D Nicolella, Ricardo A Furtado, Denise C Tavares, Thayna S Silva, Carlos H G Martins, Mayker L D Miranda
In this study, the chemical composition and antibacterial and antiproliferative potential of the essential oil obtained from fresh leaves of Psidium myrtoides (PM-EO) against oral pathogens and human tumour cell lines were investigated for the first time. GC-FID and GC-MS analyses showed that trans-β-caryophyllene (30.9%), α-humulene (15.9%), α-copaene (7.8%), caryophyllene oxide (7.3%) and α-bisabolol (5.3%) are the major constituents of PM-EO. The antibacterial activity of PM-EO against a panel of oral pathogens was investigated in terms of their minimal inhibitory concentrations (MIC) using the broth microdilution method...
April 3, 2018: Natural Product Research
https://www.readbyqxmd.com/read/29567126/distribution-of-streptococcal-groups-causing-infective-endocarditis-a-descriptive-study
#8
So Lim Kim, Steven M Gordon, Nabin K Shrestha
The purpose of this study was to describe the distribution of streptococci causing infective endocarditis (IE). A total of 296 patients with definite IE admitted from July 2007 to December 2014 were identified, with microbial identification done by a combination of blood culture, valve culture, and valve polymerase chain reaction (PCR). The overall distribution of streptococci was 76% viridans (n = 224), 17% pyogenic (50), 6% nutritionally variant (17), and 2% anaerobic (5). Sixty-three (21%) viridans group streptococci were not identified further...
February 24, 2018: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/29566581/investigation-of-bacteremia-after-debonding-procedures
#9
Yasin Akbulut, Merve Goymen, Yasemin Zer, Ayse Buyuktas Manay
OBJECTIVE: The purpose of this study is to investigate the effect of debonding procedures after completion of orthodontic treatments on bacteremia. MATERIALS AND METHODS: Twenty-eight patients who were treated with fixed orthodontic treatment at the Faculty of Dentistry's Department of Orthodontics at Gaziantep University and who had an indication of debonding were selected for this study, and blood samples were taken from these patients at different times and examined for bacteremia...
March 23, 2018: Acta Odontologica Scandinavica
https://www.readbyqxmd.com/read/29564813/omental-abscess-due-to-a-spilled-gallstone-after-laparoscopic-cholecystectomy
#10
Takeshi Urade, Hidehiro Sawa, Koichi Murata, Yasuhiko Mii, Yoshiteru Iwatani, Ryoko Futai, Shohei Abe, Tsuyoshi Sanuki, Yukiko Morinaga, Daisuke Kuroda
Omental abscess due to a spilled gallstone is extremely rare after laparoscopic cholecystectomy. Herein, we report a 68-year-old man who presented with left upper abdominal pain after laparoscopic cholecystectomy for gangrenous cholecystitis. Seven months prior to admission, gallbladder perforation with spillage of pigment gallstones and bile occurred during laparoscopic cholecystectomy. The spilled gallstones were retrieved through vigorous peritoneal lavage. Abdominal computed tomography showed a 3 × 2...
March 21, 2018: Clinical Journal of Gastroenterology
https://www.readbyqxmd.com/read/29549283/species-level-description-of-the-human-ileal-bacterial-microbiota
#11
Heidi Cecilie Villmones, Erik Skaaheim Haug, Elling Ulvestad, Nils Grude, Tore Stenstad, Adrian Halland, Øyvind Kommedal
The small bowel is responsible for most of the body's nutritional uptake and for the development of intestinal and systemic tolerance towards microbes. Nevertheless, the human small bowel microbiota has remained poorly characterized, mainly owing to sampling difficulties. Sample collection directly from the distal ileum was performed during radical cystectomy with urinary diversion. Material from the ileal mucosa were analysed using massive parallel sequencing of the 16S rRNA gene. Samples from 27 Caucasian patients were included...
March 16, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29530474/streptococcus-salivarius-mediated-cd8-t-cell-stimulation-required-antigen-presentation-by-macrophages-in-oral-squamous-cell-carcinoma
#12
Jie Wang, Lina Yang, Xiaohe Mao, Zaiye Li, Xiaoyu Lin, Canhua Jiang
It has been shown that the peripheral blood mononuclear cells (PBMCs) from oral squamous cell carcinoma (OSCC) patients presented cytotoxic CD8 T cell response against Streptococcus salivarius (S. salivarius), of which the frequency was positively associated with recurrence-free survival in OSCC patients. To identify the conditions required for regulating S. salivarius-specific CD8 T cell-mediated cytotoxicity, we selectively depleted individual components of the PBMCs, and observed that the depletion of monocytes/macrophages, but not other immune cell subsets, significantly downregulated the S...
May 15, 2018: Experimental Cell Research
https://www.readbyqxmd.com/read/29515553/identification-of-new-factors-modulating-adhesion-abilities-of-the-pioneer-commensal-bacterium-streptococcus-salivarius
#13
Benoit Couvigny, Saulius Kulakauskas, Nicolas Pons, Benoit Quinquis, Anne-Laure Abraham, Thierry Meylheuc, Christine Delorme, Pierre Renault, Romain Briandet, Nicolas Lapaque, Eric Guédon
Biofilm formation is crucial for bacterial community development and host colonization by Streptococcus salivarius , a pioneer colonizer and commensal bacterium of the human gastrointestinal tract. This ability to form biofilms depends on bacterial adhesion to host surfaces, and on the intercellular aggregation contributing to biofilm cohesiveness. Many S. salivarius isolates auto-aggregate, an adhesion process mediated by cell surface proteins. To gain an insight into the genetic factors of S. salivarius that dictate host adhesion and biofilm formation, we developed a screening method, based on the differential sedimentation of bacteria in semi-liquid conditions according to their auto-aggregation capacity, which allowed us to identify twelve mutations affecting this auto-aggregation phenotype...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29489859/emollient-use-alters-skin-barrier-and-microbes-in-infants-at-risk-for-developing-atopic-dermatitis
#14
RANDOMIZED CONTROLLED TRIAL
Martin Glatz, Jay-Hyun Jo, Elizabeth A Kennedy, Eric C Polley, Julia A Segre, Eric L Simpson, Heidi H Kong
BACKGROUND: Emollients are a mainstay of treatment in atopic dermatitis (AD), a disease distinguished by skin bacterial dysbiosis. However, changes in skin microbiota when emollients are used as a potential AD preventative measure in infants remain incompletely characterized. RESULTS: We compared skin barrier parameters, AD development, and bacterial 16S ribosomal RNA gene sequences of cheek, dorsal and volar forearm samples from 6-month-old infants with a family history of atopy randomized to receive emollients (n = 11) or no emollients (controls, n = 12)...
2018: PloS One
https://www.readbyqxmd.com/read/29465736/fermentation-of-non-digestible-raffinose-family-oligosaccharides-and-galactomannans-by-probiotics
#15
Barbara Zartl, Karina Silberbauer, Renate Loeppert, Helmut Viernstein, Werner Praznik, Monika Mueller
Due to their prebiotic potential indigestible oligosaccharides became a major focus of research interest. In this study the growth of selected probiotic strains including lactobacilli, bifidobacteria, Lactococcus lactis, Streptococcus salivarius ssp. thermophilus, Pediococcus ssp. and Enterococcus faecium with the, raffinose family oligosaccharides (RFOs) raffinose, stachyose and verbascose and galactomannan from guar bean Cyamopsis tetragonoloba (total guar carbohydrates, oligosaccharides (dp 2-4) and polysaccharides (dp > 5), obtained by size exclusion chromatography) were tested by means of turbidity measurements...
March 1, 2018: Food & Function
https://www.readbyqxmd.com/read/29464500/assessment-of-efficacy-of-blis-producing-probiotic-k12-for-the-prevention-of-group-a-streptococcus-pharyngitis-a-short-communication
#16
Francesco Di Pierro
The neutral outcome of the recently reported school-based trial of probiotic K12 (The effect of the oral probiotic Streptococcus salivarius K12 on group A streptococcus pharyngitis: a pragmatic trial in schools) can be attributed at least partially to several readily identifiable confounding factors. Mainly, the execution and outcome were negatively impacted by (a) the suboptimal efficacy and frequency of K12 administration, (b) the failure both clinically and microbiologically to adequately diagnose and distinguish active group A streptococci (GAS) pharyngitis from harmless GAS carriage, and ( c ) the exceptionally low occurrence of GAS in this population at the time of the probiotic intervention due to recent high-intensity antibiotic exposure...
February 20, 2018: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/29451932/in-vitro-screening-of-the-antibacterial-and-anti-candida-properties-of-crushed-nonantimicrobial-drugs-frequently-prescribed-in-nursing-homes
#17
Julie Lamure, Marlène Chevalier, Pascal Rathelot, Françoise Mignolet, Isabelle Precheur
Frail older adults often experience swallowing disorders, prompting nursing staff to crush tablets, open capsules, and mix drugs into their meals or gelled water. However, crushing drugs can lead to pharmacological and gustatory problems. As crushed drugs can stay in prolonged contact with oral microbial biofilm, the current study aimed to investigate their antimicrobial properties. Crushed drugs were diluted in 1 mL of isotonic water and assayed in vitro for: (a) growth inhibition of five bacterial strains and Candida albicans by the diffusion method; (b) inhibition of Streptococcus salivarius and C...
March 1, 2018: Research in Gerontological Nursing
https://www.readbyqxmd.com/read/29444418/circuitry-rewiring-directly-couples-competence-to-predation-in-the-gut-dweller-streptococcus-salivarius
#18
Johann Mignolet, Laetitia Fontaine, Andrea Sass, Catherine Nannan, Jacques Mahillon, Tom Coenye, Pascal Hols
Small distortions in transcriptional networks might lead to drastic phenotypical changes, especially in cellular developmental programs such as competence for natural transformation. Here, we report a pervasive circuitry rewiring for competence and predation interplay in commensal streptococci. Canonically, in streptococci paradigms such as Streptococcus pneumoniae and Streptococcus mutans, the pheromone-based two-component system BlpRH is a central node that orchestrates the production of antimicrobial compounds (bacteriocins) and incorporates signal from the competence activation cascade...
February 13, 2018: Cell Reports
https://www.readbyqxmd.com/read/29442170/surface-proteins-involved-in-the-adhesion-of-streptococcus-salivarius-to-human-intestinal-epithelial-cells
#19
Fanny Chaffanel, Florence Charron-Bourgoin, Claire Soligot, Mounira Kebouchi, Stéphane Bertin, Sophie Payot, Yves Le Roux, Nathalie Leblond-Bourget
The adhesion properties of 14 Streptococcus salivarius strains to mucus (HT29-MTX) and non-mucus secreting (Caco-2/TC7) human intestinal epithelial cells were investigated. Ability to adhere to these two eukaryotic cell lines greatly differs between strains. The presence of mucus played a major factor in adhesion, likely due to high adhesiveness to mucins present in the native human mucus layer covering the whole cell surface. Only one S. salivarius strain (F6-1), isolated from the feces of a healthy baby, was found to strongly adhere to HT-29 MTX cells at a level comparable to that of Lactobacillus rhamnosus GG, a probiotic strain considered to be highly adherent...
March 2018: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29412250/susceptibility-of-cariogenic-microorganisms-to-phytoconstituents
#20
G L S Ferreira, L M D Bezerra, I L A Ribeiro, R C D Morais Júnior, R D Castro
This study aimed to evaluate the in vitro antibacterial activity of the phytochemicals thymol, linalool, and citronellol against Streptococcus mutans, Streptococcus salivarius and Streptococcus oralis. Disk diffusion screening on solid medium and measurement of the diameter of the bacterial growth inhibition halos was the technique utilized. The Minimum Inhibitory Concentration (MIC) of the substances was determined using serial substance dilutions and microdilution technique in Brain Heart Infusion culture medium...
February 1, 2018: Brazilian Journal of Biology, Revista Brasleira de Biologia
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