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Fusobacterium nucleatum

Lívia Jacovassi Tavares, Marlise Inêz Klein, Beatriz Helena Dias Panariello, Erica Dorigatti de Avila, Ana Cláudia Pavarina
Purpose: The goal of this study was to develop and validate a standardized in vitro pathogenic biofilm attached onto saliva-coated surfaces. Methods: Fusobacterium nucleatum (F. nucleatum) and Porphyromonas gingivalis (P. gingivalis) strains were grown under anaerobic conditions as single species and in dual-species cultures. Initially, the bacterial biomass was evaluated at 24 and 48 hours to determine the optimal timing for the adhesion phase onto saliva-coated polystyrene surfaces...
February 2018: Journal of Periodontal & Implant Science
Santosh K Ghosh, Zhimin Feng, Hisashi Fujioka, Renate Lux, Thomas S McCormick, Aaron Weinberg
Human beta defensins (hBDs) are small cationic peptides, expressed in mucosal epithelia and important agents of innate immunity, act as antimicrobial and chemotactic agents at mucosal barriers. In this perspective, we present evidence supporting a novel strategy by which the oral bacterium Fusobacterium nucleatum induces hBDs and other antimicrobial peptides (AMPs) in normal human oral epithelial cells (HOECs) and thereby protects them from other microbial pathogens. The findings stress (1) the physiological importance of hBDs, (2) that this strategy may be a mechanism that contributes to homeostasis and health in body sites constantly challenged with bacteria and (3) that novel properties identified in commensal bacteria could, one day, be harnessed as new probiotic strategies to combat colonization of opportunistic pathogens...
2018: Frontiers in Microbiology
Cynthia L Sears
The association between the bacterium Fusobacterium nucleatum and human colon cancer is more complicated than it first appeared.
March 13, 2018: ELife
John Repass, Elizabeth Iorns, Alexandria Denis, Stephen R Williams, Nicole Perfito, Timothy M Errington
As part of the <ext-link ext-link-type="uri" xlink:href="">Reproducibility Project: Cancer Biology</ext-link>, we published a Registered Report (Repass et al., 2016), that described how we intended to replicate an experiment from the paper ' Fusobacterium nucleatum infection is prevalent in human colorectal carcinoma' (Castellarin et al., 2012). Here we report the results. When measuring Fusobacterium nucleatum DNA by qPCR in colorectal carcinoma (CRC), adjacent normal tissue, and separate matched control tissue, we did not detect a signal for F...
March 13, 2018: ELife
Dejan Stojkovic, Marina Kostic, Marija Smiljkovic, Milena Aleksic, Perica Vasiljevic, Milos Nikolic, Marina Sokovic
The following review is oriented towards microbes linked to Alzheimer's disease (AD) and antimicrobial effect of compounds and extracts derived from aquatic organisms against specific bacteria, fungi and viruses which were found previously in patients suffering from AD. Major group of microbes linked to AD include bacteria: Chlamydia pneumoniae, Helicobacter pylori, Porphyromonas gingivalis, Fusobacterium nucleatum, Prevotella intermedia, Actinomyces naeslundii, spirochete group; fungi: Candida sp., Cryptococcus sp...
March 8, 2018: Current Medicinal Chemistry
Benoit Couvigny, Saulius Kulakauskas, Nicolas Pons, Benoit Quinquis, Anne-Laure Abraham, Thierry Meylheuc, Christine Delorme, Pierre Renault, Romain Briandet, Nicolas Lapaque, Eric Guédon
Biofilm formation is crucial for bacterial community development and host colonization by Streptococcus salivarius , a pioneer colonizer and commensal bacterium of the human gastrointestinal tract. This ability to form biofilms depends on bacterial adhesion to host surfaces, and on the intercellular aggregation contributing to biofilm cohesiveness. Many S. salivarius isolates auto-aggregate, an adhesion process mediated by cell surface proteins. To gain an insight into the genetic factors of S. salivarius that dictate host adhesion and biofilm formation, we developed a screening method, based on the differential sedimentation of bacteria in semi-liquid conditions according to their auto-aggregation capacity, which allowed us to identify twelve mutations affecting this auto-aggregation phenotype...
2018: Frontiers in Microbiology
Yaxin Wu, Jiao Wu, Ting Chen, Qing Li, Wei Peng, Huan Li, Xiaowei Tang, Xiangsheng Fu
BACKGROUND: The underlying pathogenic mechanism of Fusobacterium nucleatum in the carcinogenesis of colorectal cancer has been poorly understood. METHODS: Using C57BL/6-ApcMin/+ mice, we investigated gut microbial structures with F. nucleatum, antibiotics, and Toll-like receptor 4 (TLR4) antagonist TAK-242 treatment. In addition, we measured intestinal tumor formation and the expression of TLR4, p21-activated kinase 1 (PAK1), phosphorylated-PAK1 (p-PAK1), phosphorylated-β-catenin S675 (p-β-catenin S675), and cyclin D1 in mice with different treatments...
March 5, 2018: Digestive Diseases and Sciences
A C S Marinho, T T To, R P Darveau, B P F A Gomes
AIM: To investigate the influence of auxiliary chemical substances (ACS) and calcium hydroxide [Ca(OH)2 ] dressings on LPS/lipid A detection and its functional ability in activating Toll-like receptor 4 (TLR4). METHODOLOGY: Fusobacterium nucleatum pellets were exposed to antimicrobial agents as following: 1) ACS: 5.25%, 2.5% and 1% sodium hypochlorite solutions (NaOCl), 2% chlorhexidine (CHX) (gel and solution) and 17% ethylenediaminetetraacetic acid (EDTA); 2) Intracanal medicament: Ca(OH)2 paste for various periods (1h, 24h, 7 days, 14 days and 30 days); 3) Combination of substances: a) 2...
March 5, 2018: International Endodontic Journal
J Zhang, X L Lu, G Zhao, H T Shi, Y Geng, W T Zhong, L Dong
Objective: To explore relationships between the enrichment of ETBF, Fn, Hp in feces, tissues and colorectal cancer. Methods: Feces, lesion tissue and adjacent tissue from 24 patients with colorectal cancer and 31 patients with adenomas were collected, and we collected Feces and tissue of 20 healthy control persons. Then the copy numbers of enterotoxigenic B. fragilis (ETBF), Fusobacterium nucleatum (Fn) and Helicobacter pylori (Hp) were determined by quantitative real-time PCR. Immunohistochemical method was used to examine the expression intensity of EGFR and p53, and the relationships between different expression intensity of EGFR, p53 and the numbers of three bacterias...
February 23, 2018: Zhonghua Zhong Liu za Zhi [Chinese Journal of Oncology]
Ying Xue, Han Xiao, Songhe Guo, Banglao Xu, Yuehua Liao, Yixian Wu, Ge Zhang
Fusobacterium nucleatum (Fn) is a tumor-associated obligate anaerobic bacterium, which has a role in the progression of colorectal cancer (CRC). Fn can invade and promote colon epithelial cells proliferation. However, how Fn survives and proliferates in its host cells remains largely unknown. In this study, we aimed to determine the molecular mechanisms underlying the morphology, survival, and proliferation of Fn in THP-1-derived macrophages (dTHP1). For the first time, we found that Fn is a facultative intracellular bacterium that can survive and limited proliferate in dTHP1 cells up to 72 h, and a live Fn infection can inhibit apoptosis of dTHP1 cells by activating the PI3K and ERK pathways...
March 2, 2018: Cell Death & Disease
Ren-Chao Zheng, Xiao-Ling Tang, Hui Suo, Li-Lin Feng, Xiao Liu, Jian Yang, Yu-Guo Zheng
Tyrosine phenol-lyase (TPL) catalyzes the reversible cleavage of l-tyrosine to phenol, pyruvate and ammonia. When pyrocatechol is substituted for phenol, l-dihydroxyphenylalanine (l-DOPA) is produced. The TPL-catalyzed route was regarded as the most economic process for l-DOPA production. In this study, a novel TPL from Fusobacterium nucleatum (Fn-TPL) was successfully overexpressed in Escherichia coli and screened for l-DOPA synthesis with a specific activity of 2.69Umg-1 . Fn-TPL was found to be a tetramer, and the optimal temperature and pH for α, β-elimination of l-tyrosine was 60°C and pH 8...
May 2018: Enzyme and Microbial Technology
Julen Tomás-Cortázar, Laura Plaza-Vinuesa, Blanca de Las Rivas, José Luis Lavín, Diego Barriales, Leticia Abecia, José Miguel Mancheño, Ana M Aransay, Rosario Muñoz, Juan Anguita, Héctor Rodríguez
BACKGROUND: Tannases are tannin-degrading enzymes that have been described in fungi and bacteria as an adaptative mechanism to overcome the stress conditions associated with the presence of these phenolic compounds. RESULTS: We have identified and expressed in E. coli a tannase from the oral microbiota member Fusobacterium nucleatum subs. polymorphum (TanBFnp ). TanBFnp is the first tannase identified in an oral pathogen. Sequence analyses revealed that it is closely related to other bacterial tannases...
February 26, 2018: Microbial Cell Factories
Thomas Thurnheer, Georgios N Belibasakis
OBJECTIVE: Fluoride is widely used as an anti-caries agent, e.g. in toothpastes and mouth rinses. However, the nature of the anti-caries action is not entirely clear. Mechanisms suspected to explain the cariostatic effect include inhibitory effects on acid formation by bacteria, inhibition of extracellular polysaccharide (EPS) production, inhibition of enamel demineralization and enhancement of remineralizaton or combination thereof. The aim of this study was to examine with the supragingival Zurich in vitro biofilm model the effect of fluoride in NaF formulation, on the microbiota and on demineralization...
February 20, 2018: Archives of Oral Biology
Zhi-Luo Deng, Helena Sztajer, Michael Jarek, Sabin Bhuju, Irene Wagner-Döbler
Periodontitis is a worldwide prevalent oral disease which results from dysbiosis of the periodontal microbiome. Some of the most active microbial players, e.g., Porphyromonas gingivalis, Treponema denticola , and Fusobacterium nucleatum , have extensively been studied in the laboratory, but it is unclear to which extend these findings can be transferred to in vivo conditions. Here we show that the transcriptional profiles of P. gingivalis, T. denticola , and F. nucleatum in the periodontal niche are distinct from those in single laboratory culture and exhibit functional similarities...
2018: Frontiers in Microbiology
Adelaida Esteban-Fernández, Irene Zorraquín-Peña, Maria D Ferrer, Alex Mira, Begoña Bartolomé, Dolores González de Llano, M Victoria Moreno-Arribas
Several benefits have been described for red wine polyphenols and probiotic strains in the promotion of colonic metabolism and health. On the contrary, knowledge about their role in the management of oral health is still scarce. In this work, the antiadhesive capacity of selected red wine polyphenols and oenological extracts against the oral pathogens Porphyromonas gingivalis, Fusobacterium nucleatum, and Streptococcus mutans in an in vitro model of human gingival fibroblasts has been explored as well as their complementary action with the candidate oral probiotic Streptococcus dentisani...
February 21, 2018: Journal of Agricultural and Food Chemistry
D Lauritano, V Candotto, C A Bignozzi, D Pazzi, F Carinci, F Cura, A Tagliabue, L Tettamanti
Periodontal treatment has the aim to reduce oral infection, and prevent the progression of the disease. The potential benefits of new chemical devices for periodontal therapy, include improved patient compliance, an easier access to periodontal pocket and a lower dosage of antimicrobial agent. The objective of this study was to explore the efficacy of a chemical device containing zinc and octenidine in the treatment of chronic periodontitis in adult patients. Ten patients with a diagnosis of chronic periodontitis (20 localized chronic periodontitis sites) in the age group of 35 to 55 were selected...
January 2018: Journal of Biological Regulators and Homeostatic Agents
Ali Al-Ahmad, Fariba Muzafferiy, Annette C Anderson, Johan P Wölber, Petra Ratka-Krüger, Tobias Fretwurst, Katja Nelson, Kirstin Vach, Elmar Hellwig
PURPOSE: Micro-organisms are important triggers of peri-implant inflammation and analysing their diversity is necessary for peri-implantitis treatment. This study aimed to analyse and compare the microbiota associated with individuals with peri-implantitis, as well as clinically healthy implant sites. METHODOLOGY: Subgingival biofilm samples were taken from 10 individuals with peri-implantitis and from at least 1 clinically healthy implant. DNA was extracted and bacterial 16S rRNA genes were amplified using universal primers...
March 2018: Journal of Medical Microbiology
Hongye Lu, Lu He, Yibing Zhao, Huanxin Meng
Background: Glycine air polishing has been proved to be safe, comfortable and time-saving. Whether it could substitute ultrasonic scaling to remove dental plaque biofilm during periodontal maintenance remains unclear. The purposes of this study were to evaluate the effect of supragingival glycine air polishing (SGAP) on the subgingival periodontal pathogens during maintenance therapy and to check the association of periodontal pathogens and clinical parameters. Methods: Twenty-three chronic periodontitis patients during their maintenance therapy were enrolled in the 12-week study...
2018: PeerJ
Multazim Muradkhan Pathan, Kishore Gajanan Bhat, Vinayak Mahableshwar Joshi
Background: Several herbal mouthwash and herbal extracts have been tested in vitro and in vivo in search of a suitable adjunct to mechanical therapy for long-term use. In this study, we aimed to look at the antimicrobial effect of the herbal mouthwash and chlorhexidine (CHX) mouthwash on select organisms in in vitro test and an ex vivo model. Materials and Methods: The antimicrobial effects were determined against standard strains of bacteria that are involved in different stages of periodontal diseases...
July 2017: Journal of Indian Society of Periodontology
Beatrice Gutt, Qun Ren, Irmgard Hauser-Gerspach, Piotr Kardas, Stefan Stübinger, Monika Astasov-Frauenhoffer, Tuomas Waltimo
Periodontitis is a very common health problem caused by formation of pathogenic bacterial biofilm that triggers inflammation resulting in either reversible gingivitis or irreversible periodontal hard and soft tissue damages, leading to loss of teeth when left untreated. Commensal bacteria play an important role in oral health in many aspects. Mainly by colonizing oral tissues, they (i) contribute to maturation of immune response, and (ii) foreclose attachment of pathobiont and, therefore, prevent from infection...
2018: Frontiers in Microbiology
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