Michaela J Conley, Judith M Short, Andrew M Burns, James Streetley, Joshua Hutchings, Saskia E Bakker, B Joanne Power, Hussain Jaffery, Joanne Haney, Giulia Zanetti, Pablo R Murcia, Murray Stewart, Rachel Fearns, Swetha Vijayakrishnan, David Bhella
Human respiratory syncytial virus (RSV) causes severe respiratory illness in children and the elderly. Here, using cryogenic electron microscopy and tomography combined with computational image analysis and three-dimensional reconstruction, we show that there is extensive helical ordering of the envelope-associated proteins and glycoproteins of RSV filamentous virions. We calculated a 16 Å resolution sub-tomogram average of the matrix protein (M) layer that forms an endoskeleton below the viral envelope...
February 1, 2022: EMBO Journal