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https://www.readbyqxmd.com/read/27913727/regulated-complex-assembly-safeguards-the-fidelity-of-sleeping-beauty-transposition
#1
Yongming Wang, Diana Pryputniewicz-Dobrinska, Enikö Éva Nagy, Christopher D Kaufman, Manvendra Singh, Steve Yant, Jichang Wang, Anna Dalda, Mark A Kay, Zoltán Ivics, Zsuzsanna Izsvák
The functional relevance of the inverted repeat structure (IR/DR) in a subgroup of the Tc1/mariner superfamily of transposons has been enigmatic. In contrast to mariner transposition, where a topological filter suppresses single-ended reactions, the IR/DR orchestrates a regulatory mechanism to enforce synapsis of the transposon ends before cleavage by the transposase occurs. This ordered assembly process shepherds primary transposase binding to the inner 12DRs (where cleavage does not occur), followed by capture of the 12DR of the other transposon end...
December 1, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/27912968/validation-of-a-thin-layer-chromatography-densitometry-method-for-the-characterization-of-invertase-activity
#2
Justine Ferey, David Da Silva, Sophie Bravo-Veyrat, Pierre Lafite, Richard Daniellou, Benoît Maunit
This paper presents a kinetic study of invertase, a specific fructofuranosidase cloned from the Leishmania major genome. The kinetic parameters of the β-d-fructofuranosidase from Leishmania major (BfrA) were determined using Thin-Layer Chromatography (TLC) and UV-densitometry (TLC@UV) specifically developed for the separation and detection of three carbohydrates namely sucrose, glucose and fructose. Separation was performed on TLC silica gel 60 F254 plates impregnated with sodium bisulphate and citrate and heated prior to development...
November 25, 2016: Journal of Chromatography. A
https://www.readbyqxmd.com/read/27912190/nanostructured-3d-porous-graphene-hydrogel-based-ti-sb-sno2-gr-electrode-with-enhanced-electrocatalytic-activity
#3
Sumreen Asim, Yunqing Zhu, Masud Rana, Jiao Yin, Muhammad Wajid Shah, Yingxuan Li, Chuanyi Wang
Nanostructured highly porous 3D-Ti/Sb-SnO2-Gr electrode, based on 3D porous graphene hydrogel was fabricated via a fast-evaporation technique through layer by layer (LBL) deposition. The 3D pores are uniformly distributed on the high fidelity of substrate with pore sizes of 7-12 nm, as confirmed by SEM analysis. Compared to Ti/Sb-SnO2 electrode, the fabricated 3D porous electrode possesses high oxygen evolution potential (2.40 V), smaller charge transfer resistance (29.40 Ω cm(-2)), higher porosity (0...
November 29, 2016: Chemosphere
https://www.readbyqxmd.com/read/27912140/characterization-of-the-cleaved-edge-cross-section-of-the-heterostructures-with-gamnas-layer-by-the-confocal-micro-raman-spectroscopy
#4
A V Kudrin, S M Plankina, O V Vikhrova, A V Nezhdanov, A I Mashin, Yu N Drozdov, A V Shvetsov
Confocal micro-Raman spectroscopy was used to measure cross-section linescans of the cleaved edge of heterostructures involving a GaMnAs layer. The investigations revealed a shift of the TO mode in the compressed GaMnAs layer to high frequencies relative to the TO GaAs mode in the substrate and to low frequencies in the tensile GaMnAs layers. These results are in agreement with the different manifestations of the anomalous Hall effect in the GaMnAs layers, with either compressive or tensile strains. It is shown that Raman spectroscopy is an appropriate method for the investigation of cross-sectional semiconductor heterostructures whose total thickness is comparable to the size of the analyzing laser spot...
November 21, 2016: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/27911771/stability-folding-dynamics-and-long-range-conformational-transition-of-the-synaptic-t-snare-complex
#5
Xinming Zhang, Aleksander A Rebane, Lu Ma, Feng Li, Junyi Jiao, Hong Qu, Frederic Pincet, James E Rothman, Yongli Zhang
Synaptic soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) couple their stepwise folding to fusion of synaptic vesicles with plasma membranes. In this process, three SNAREs assemble into a stable four-helix bundle. Arguably, the first and rate-limiting step of SNARE assembly is the formation of an activated binary target (t)-SNARE complex on the target plasma membrane, which then zippers with the vesicle (v)-SNARE on the vesicle to drive membrane fusion. However, the t-SNARE complex readily misfolds, and its structure, stability, and dynamics are elusive...
November 28, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27911433/-medical-terminology-blepharorhaphy
#6
E V Boiko, V F Chernysh
: Ophthalmic literature currently lacks definiteness in classifying surgeries aimed at eyelid closure. In Western countries the term tarsorrhaphy is most commonly used, while in Russia another term - blepharorhaphy - is widely accepted. The latter seems more appropriate as the sutures are passed through all palpebral layers, not just the cartilage. It has also been suggested that temporary blepharorhaphy, which implies that we use only sutures to bring the eyelids together, and therefore, the procedure cannot be permanent, should be called simple blepharorhaphy...
2016: Vestnik Oftalmologii
https://www.readbyqxmd.com/read/27911422/-diagnostic-potential-of-optical-coherence-tomography-for-small-choroidal-melanomas
#7
A F Brovkina, A S Stoyukhina, I V Musatkina
AIM: To investigate the sequence of OCT manifestations in choroidal melanoma (CM) with account to its growth. MATERIAL AND METHODS: A total of 28 CM patients were examined. Of them, 18 patients with early CM, 10 - with medium-sized CM. The mean patient age at diagnosis was 60.54±5.31 years. Tumor location was macular (15 eyes) or perimacular (13 eyes), its prominence varied from 0.98 to 4.9 mm and basal diameter - from 5.08 to 18.9 mm. Additionally to OCT, tumor autofluorescence was studied in all cases...
2016: Vestnik Oftalmologii
https://www.readbyqxmd.com/read/27911421/-analysis-of-changes-in-central-macular-thickness-based-on-optical-coherence-tomography-angiography-findings-in-retinal-vein-occlusion
#8
M V Budzinskaya, A V Shelankova, M A Mikhailova, A A Plyukhova, N M Nurieva, A V Fomin
AIM: To investigate the relationship between OCT angiography measurements and central fundus changes in patients with retinal vein occlusion (RVO). MATERIAL AND METHODS: The study enrolled 21 RVO patients aged from 55 to 86 years (69.9±2.28 years), including 8 patients with ischemic central RVO (I-CRVO; 73.6±3.4 years on average) and 13 patients with branch RVO (BRVO; 67.6±3.0 years on average). Of the latter, 8 cases were ischemic (I-BRVO) and 5 non-ischemic (NI-BRVO)...
2016: Vestnik Oftalmologii
https://www.readbyqxmd.com/read/27911417/plasma-assisted-molecular-beam-epitaxy-of-n-polar-inaln-barrier-high-electron-mobility-transistors
#9
Matthew T Hardy, David F Storm, D Scott Katzer, Brian P Downey, Neeraj Nepal, David J Meyer
Plasma-assisted molecular beam epitaxy is well suited for the epitaxial growth of III-nitride thin films and heterostructures with smooth, abrupt interfaces required for high-quality high-electron-mobility transistors (HEMTs). A procedure is presented for the growth of N-polar InAlN HEMTs, including wafer preparation and growth of buffer layers, the InAlN barrier layer, AlN and GaN interlayers and the GaN channel. Critical issues at each step of the process are identified, such as avoiding Ga accumulation in the GaN buffer, the role of temperature on InAlN compositional homogeneity, and the use of Ga flux during the AlN interlayer and the interrupt prior to GaN channel growth...
November 24, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27911375/fabrication-of-white-light-emitting-electrochemical-cells-with-stable-emission-from-exciplexes
#10
Soichi Uchida, Daisuke Takizawa, Satoru Ikeda, Hironori Takeuchi, Suzushi Nishimura, Hiroyuki Nishide, Yoshinori Nishikitani
The authors present an approach for fabricating stable white light emission from polymer light-emitting electrochemical cells (PLECs) having an active layer which consists of blue-fluorescent poly(9,9-di-n-dodecylfluorenyl-2,7-diyl) (PFD) and π-conjugated triphenylamine molecules. This white light emission originates from exciplexes formed between PFD and amines in electronically excited states. A device containing PFD, 4,4',4''-tris[2-naphthyl(phenyl)amino]triphenylamine (2-TNATA), Poly(ethylene oxide) and K2CF3SO3 showed white light emission with Commission internationale de l'éclairage (CIE) coordinates of (0...
November 15, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27910917/metal-induced-growth-of-transition-metal-dichalcogenides-at-controlled-locations
#11
Zhendong Wang, Qi Huang, Peng Chen, Shouhui Guo, Xiaoqing Liu, Xuelei Liang, Li Wang
Metal induced nucleation is adopted to achieve the growth of transition metal dichalcogenides at controlled locations. Ordered arrays of MoS2 and WS2 have successfully been fabricated on SiO2 substrates by using the patterned Pt/Ti dots as the nucleation sites. Uniform MoS2 monolayers with the adjustable size up to 50 μm are grown surrounding these metal patterns and the mobility of such layer is about 0.86 cm(2)/V·s. The crystalline flakes of WS2 are also fabricated extending from the metal patterns and the electron mobility of these flakes is up to 11...
December 2, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27910673/implementation-of-the-new-multichannel-x-mode-edge-density-profile-reflectometer-for-the-icrf-antenna-on-asdex-upgrade
#12
D E Aguiam, A Silva, V Bobkov, P J Carvalho, P F Carvalho, R Cavazzana, G D Conway, O D'Arcangelo, L Fattorini, H Faugel, A Fernandes, H Fünfgelder, B Gonçalves, L Guimarais, G De Masi, L Meneses, J M Noterdaeme, R C Pereira, G Rocchi, J M Santos, A A Tuccillo, O Tudisco
A new multichannel frequency modulated continuous-wave reflectometry diagnostic has been successfully installed and commissioned on ASDEX Upgrade to measure the plasma edge electron density profile evolution in front of the Ion Cyclotron Range of Frequencies (ICRF) antenna. The design of the new three-strap ICRF antenna integrates ten pairs (sending and receiving) of microwave reflectometry antennas. The multichannel reflectometer can use three of these to measure the edge electron density profiles up to 2 × 10(19) m(-3), at different poloidal locations, allowing the direct study of the local plasma layers in front of the ICRF antenna...
November 2016: Review of Scientific Instruments
https://www.readbyqxmd.com/read/27910447/design-of-the-polar-neutron-imaging-aperture-for-use-at-the-national-ignition-facility
#13
V E Fatherley, D A Barker, D N Fittinghoff, R L Hibbard, J I Martinez, F E Merrill, J A Oertel, D W Schmidt, P L Volegov, C H Wilde
The installation of a neutron imaging diagnostic with a polar view at the National Ignition Facility (NIF) required design of a new aperture, an extended pinhole array (PHA). This PHA is different from the pinhole array for the existing equatorial system due to significant changes in the alignment and recording systems. The complex set of component requirements, as well as significant space constraints in its intended location, makes the design of this aperture challenging. In addition, lessons learned from development of prior apertures mandate careful aperture metrology prior to first use...
November 2016: Review of Scientific Instruments
https://www.readbyqxmd.com/read/27910357/diagnostics-for-molybdenum-and-tungsten-erosion-and-transport-in-nstx-u
#14
F Scotti, V A Soukhanovskii, M E Weller
A comprehensive set of spectroscopic diagnostics is planned in the National Spherical Torus Experiment Upgrade to connect measurements of molybdenum and tungsten divertor sources to scrape-off layer (SOL) and core impurity transport, supporting the installation of high-Z plasma facing components which is scheduled to begin with a row of molybdenum tiles. Imaging with narrow-bandpass interference filters and high-resolution spectroscopy will be coupled to estimate divertor impurity influxes. Vacuum ultraviolet and extreme ultraviolet spectrometers will allow connecting high-Z sources to SOL transport and core impurity content...
November 2016: Review of Scientific Instruments
https://www.readbyqxmd.com/read/27910344/icrf-wave-field-measurements-in-the-presence-of-scrape-off-layer-turbulence-on-the-asdex-upgrade-tokamak-invited
#15
R Ochoukov, V Bobkov, H Faugel, H Fünfgelder, J Jacquot, J-M Noterdaeme, G Suárez López
A new array of B-dot probes was installed on ASDEX Upgrade. The purpose of the new diagnostic is to study Ion Cyclotron Range-off Frequencies (ICRF) wave field distributions in the evanescent scrape-off layer (SOL) plasma region on the low field side of ASDEX Upgrade. The vacuum measurements (no gas, BT = 0 T) reveal ICRF wave field measurements consistent with the profiles expected from the newly installed 3-strap ICRF antennas outside the antenna box: the shape of the toroidal distribution of both the amplitude and the phase is the same for the case of only the central straps being active, as for the case of only the side straps being active...
November 2016: Review of Scientific Instruments
https://www.readbyqxmd.com/read/27910115/an-expert-consensus-definition-of-failure-of-a-treatment-to-provide-adequate-relief-f-par-for-chronic-constipation-an-international-delphi-survey
#16
J Tack, H Boardman, P Layer, I Schiefke, D Jayne, C Scarpignato, M Fox, T Frieling, P Ducrotte, S Hamdy, K Gill, C Ciriza de Los Rios, R Felt-Bersma, D De Looze, V Stanghellini, A Mohr Drewes, M Simrén, C Pehl, T Hoheisel, A Leodolter, E Rey, J Dalrymple, A Emmanuel
BACKGROUND: As treatments for constipation become increasingly available, it is important to know when to progress along the treatment algorithm if the patient is not better. AIM: To establish the definition of failure of a treatment to provide adequate relief (F-PAR) to support this management and referral process in patients with chronic constipation. METHODS: We conducted an international Delphi Survey among gastroenterologists and general practitioners with a special interest in chronic constipation...
December 1, 2016: Alimentary Pharmacology & Therapeutics
https://www.readbyqxmd.com/read/27909313/nanoscale-self-templating-for-oxide-epitaxy-with-large-symmetry-mismatch
#17
Xiang Gao, Shinbuhm Lee, John Nichols, Tricia L Meyer, Thomas Z Ward, Matthew F Chisholm, Ho Nyung Lee
Direct observations using scanning transmission electron microscopy unveil an intriguing interfacial bi-layer that enables epitaxial growth of a strain-free, monoclinic, bronze-phase VO2(B) thin film on a perovskite SrTiO3 (STO) substrate. We observe an ultrathin (2-3 unit cells) interlayer best described as highly strained VO2(B) nanodomains combined with an extra (Ti,V)O2 layer on the TiO2 terminated STO (001) surface. By forming a fully coherent interface with the STO substrate and a semi-coherent interface with the strain-free epitaxial VO2(B) film above, the interfacial bi-layer enables the epitaxial connection of the two materials despite their large symmetry and lattice mismatch...
December 2, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27908112/oscillating-electric-field-effects-on-adsorbed-water-at-rutile-and-anatase-tio2-surfaces
#18
Zdenek Futera, Niall J English
We have performed non-equilibrium molecular dynamics simulations of various TiO2/water interfaces at ambient temperature in presence of oscillating electric fields in frequency range 20-100 GHz and RMS intensities 0.05-0.25 V/Å. Although the externally applied fields are by one order of magnitude lower than the intrinsic electric field present on the interfaces (∼1.5-4.5 V/Å), significant non-thermal coupling of rotational and translational motion of water molecules was clearly observed. Enhancement of the motion, manifested by increase of diffusivity, was detected in the first hydration layer, which is known to be heavily confined by adsorption to the TiO2 surface...
November 28, 2016: Journal of Chemical Physics
https://www.readbyqxmd.com/read/27908109/perturbation-of-hydration-layer-in-solvated-proteins-by-external-electric-and-electromagnetic-fields-insights-from-non-equilibrium-molecular-dynamics
#19
Prithwish K Nandi, Zdenek Futera, Niall J English
Given the fundamental role of water in governing the biochemistry of enzymes, and in regulating their wider biological activity (e.g., by local water concentration surrounding biomolecules), the influence of extraneous electric and electromagnetic (e/m) fields thereon is of central relevance to biophysics and, more widely, biology. With the increase in levels of local and atmospheric microwave-frequency radiation present in modern life, as well as other electric-field exposure, the impact upon hydration-water layers surrounding proteins, and biomolecules generally, becomes a particularly pertinent issue...
November 28, 2016: Journal of Chemical Physics
https://www.readbyqxmd.com/read/27908003/resonance-phenomena-in-a-two-layer-two-vortex-shear-flow
#20
Eugene A Ryzhov, Konstantin V Koshel
The paper deals with a dynamical system governing the motion of two point vortices embedded in the bottom layer of a two-layer rotating flow experiencing linear deformation and their influence on fluid particle advection. The linear deformation consists of shear and rotational components. If the deformation is stationary, the vortices can move periodically in a bounded region. The vortex periodic motion induces stirring patterns of passive fluid particles in the both layers. We focus our attention on the upper layer where the bottom-layer singular point vortices induce a regular velocity field with no singularities...
November 2016: Chaos
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