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Ying Zhuge, Zhi-Feng Zheng, Mu-Qing Xie, Lin Li, Fang Wang, Feng Gao
The objective of this study was to develop an amiodarone hydrochloride (ADHC)-loaded liposome (ADHC-L) formulation and investigate its potential for cardiomyocyte targeting after cardiac radiofrequency ablation (CA) in vivo. The ADHC-L was prepared by thin-film method combined with ultrasonication and extrusion. The preparation process was optimized by Box-Behnken design with encapsulation efficiency as the main evaluation index. The optimum formulation was quantitatively obtained with a diameter of 99.9±0...
2016: International Journal of Nanomedicine
Hai-Yan Sun, Bei-Bei Liu, Jian-Yang Hu, Li-Jia Xu, Shun-Wan Chan, Chi-On Chan, Daniel K W Mok, Dong-Mei Zhang, Wen-Cai Ye, Si-Bao Chen
BACKGROUND: Cycloartane triterpenoids exhibited anticancer effects. This study aims to identify any potential novel anticancer cycloartane triterpenoids from Cimicifuga foetida L. rhizome (Sheng ma) and the mode of actions. METHODS: Cycloartane triterpenoids were isolated from the C. foetida rhizome by a series of column chromatography and identified by IR, MS and NMR. Their anticancer effects on several human cancer cell lines, MCF-7, HepG2, HepG2/ADM, HeLa, and PC3, and normal human mammary epithelial cells MCF10A were investigated by colony formation and MTT assays...
2016: Chinese Medicine
Donald Jiang, Alexandra Tikhomirova, Stephen P Kidd
An alcohol dehydrogenase, AdhC, is required for Haemophilus influenzae Rd KW20 growth with high oxygen. AdhC protects against both exogenous and metabolically generated, endogenous reactive aldehydes. However, adhC in the strain 86-028NP is a pseudogene. Unlike the Rd KW20 adhC mutant, 86-028NP does grow with high oxygen. This suggests the differences between Rd KW20 and 86-028NP include broader pathways, such as for the maintenance of redox and metabolism that avoids the toxicity related to oxygen. We hypothesized that these differences affect their resistance to relevant toxic chemicals, including reactive aldehydes...
January 2016: Research in Microbiology
Yeonsu Song, Joseph M Dzierzewski, Constance H Fung, Juan C Rodriguez, Stella Jouldjian, Michael N Mitchell, Karen R Josephson, Cathy A Alessi, Jennifer L Martin
OBJECTIVES: To examine whether sleep disturbance is associated with poor physical function in older veterans in an adult day healthcare (ADHC) program. DESIGN: Cross-sectional. SETTING: One ADHC program in a Veterans Affairs Ambulatory Care Center. PARTICIPANTS: Older veterans (N = 50) enrolled in a randomized controlled trial of a sleep intervention program who had complete baseline data. MEASUREMENTS: Information on participant characteristics (e...
August 2015: Journal of the American Geriatrics Society
Tony Florio, Julian Trollor
OBJECTIVES: The main objective of the study was to compare mortality for people with an intellectual disability (ID) to the general population in New South Wales (NSW), Australia. A second objective was to provide mortality data for people with an intellectual disability from NSW in a standardized format, which allows for international comparisons of the mortality of people with an intellectual disability. DESIGN AND SETTING: The study was a longitudinal cohort study linking retrospective data from the population databases of the NSW Department of Ageing, Disability and Home Care (ADHC) Intellectual Disability Services Dataset (intellectual disability cohort) to the mortality data set of the NSW Registry of Births, Deaths and Marriages (RBDM) for the period 1 June 2005 to 31 December 2011...
September 2015: Journal of Applied Research in Intellectual Disabilities: JARID
Daniel W Meechan, Thomas M Maynard, Eric S Tucker, Alejandra Fernandez, Beverly A Karpinski, Lawrence A Rothblat, Anthony-S LaMantia
Understanding the developmental etiology of autistic spectrum disorders, attention deficit/hyperactivity disorder and schizophrenia remains a major challenge for establishing new diagnostic and therapeutic approaches to these common, difficult-to-treat diseases that compromise neural circuits in the cerebral cortex. One aspect of this challenge is the breadth and overlap of ASD, ADHD, and SCZ deficits; another is the complexity of mutations associated with each, and a third is the difficulty of analyzing disrupted development in at-risk or affected human fetuses...
July 2015: Progress in Neurobiology
Bum Jung Kim
The purpose of this study is to examine the direct and indirect effects of Adult Day Health Care (ADHC) and family network on Quality of Life (QOL) for low-income older Korean immigrants in Los Angeles County, CA. A cross-sectional survey of low-income older Korean immigrants who use ADHC programs was conducted. Self-reported measures included sociocultural characteristics, acculturation, cognitive function, family network, utilization of ADHC, and QOL. The study found that for QOL, two variables had only direct effects: years in ADHC and acculturation...
May 2014: Research on Aging
Matthias Raberg, Birgit Voigt, Michael Hecker, Alexander Steinbüchel
The undefined poly(3-hydroxybutyrate)- (PHB-) negative mutant R. eutropha PHB-4 was generated in 1970 by 1-nitroso-3-nitro-1-methylguanidine (NMG) treatment. Although being scientific relevant, its genotype remained unknown since its isolation except a recent first investigation. In this study, the mutation causing the PHA-negative phenotype of R. eutropha PHB-4 was confirmed independently: sequence analysis of the phaCAB operon identified a G320A mutation in phaC yielding a stop codon, leading to a massively truncated PhaC protein of 106 amino acids (AS) in R...
2014: PloS One
Biswajit Saha, S M Wahidur Rahaman, Prosenjit Daw, Gargi Sengupta, Jitendra K Bera
Metal-metal singly-bonded diruthenium complexes, bridged by naphthyridine-functionalized N-heterocyclic carbene (NHC) ligands featuring a hydroxy appendage on the naphthyridine unit, are obtained in a single-pot reaction of [Ru2(CH3COO)2(CO)4] with 1-benzyl-3-(5,7-dimethyl-1,8-naphthyrid-2-yl)imidazolium bromide (BIN⋅HBr) or 1-isopropyl-3-(5,7-dimethyl-1,8-naphthyrid-2-yl)imidazolium bromide (PIN⋅HBr), TlBF4, and substituted benzaldehyde containing an electron-withdrawing group. The modified NHC-naphthyridine-hydroxy ligand spans the diruthenium unit in which the NHC carbon and hydroxy oxygen occupy the axial sites...
May 19, 2014: Chemistry: a European Journal
Jaime M Hughes, Jennifer L Martin
Addressing sleep disturbance can help to slow functional decline, delay nursing home admission, and improve overall health among older adults; however, sleep is not widely studied in high-risk older adults such as Adult Day Health Care (ADHC) participants. Sixty-eight ADHC participants were interviewed for sleep disturbance using a 28-item screening questionnaire. More than two thirds (n = 48, 70.6%) reported one or more characteristics of poor sleep, and 38% of participants met basic criteria for insomnia...
2015: Behavioral Sleep Medicine
Shun Sato, Naoki Morita, Dai Kitamoto, Hiroshi Habe
Some acetic acid bacteria have been shown to produce large amounts of glyceric acid (GA) from glycerol, which is a by-product of biodiesel fuel (BDF) production. Previously, a Gluconobacter strain was found that produced decreased amounts of GA from glycerol in the presence of methanol, a major ingredient of raw glycerol derived from the BDF industry. Thus, a comparative transcriptome analysis of Gluconobacter frateurii NBRC103465 was performed to investigate changes in gene expression during GA production from glycerol in the presence of methanol...
2013: Journal of Oleo Science
Sheila Z Kimaro Mlacha, Sandra Romero-Steiner, Julie C Dunning Hotopp, Nikhil Kumar, Nadeeza Ishmael, David R Riley, Umar Farooq, Todd H Creasy, Luke J Tallon, Xinyue Liu, Cynthia S Goldsmith, Jacquelyn Sampson, George M Carlone, Susan K Hollingshead, J Anthony G Scott, Hervé Tettelin
BACKGROUND: Streptococcus pneumoniae is a leading cause of childhood morbidity and mortality worldwide, despite the availability of effective pneumococcal vaccines. Understanding the molecular interactions between the bacterium and the host will contribute to the control and prevention of pneumococcal disease. RESULTS: We used a combination of adherence assays, mutagenesis and functional genomics to identify novel factors involved in adherence. By contrasting these processes in two pneumococcal strains, TIGR4 and G54, we showed that adherence and invasion capacities vary markedly by strain...
June 9, 2013: BMC Genomics
Nathan H Chen, Rafael M Couñago, Karrera Y Djoko, Michael P Jennings, Michael A Apicella, Bostjan Kobe, Alastair G McEwan
AIM: The glutathione-dependent AdhC-EstD formaldehyde detoxification system is found in eukaryotes and prokaryotes. It is established that it confers protection against formaldehyde that is produced from environmental sources or methanol metabolism. Thus, its presence in the human host-adapted bacterial pathogen Neisseria meningitidis is intriguing. This work defined the biological function of this system in the meningococcus using phenotypic analyses of mutants linked to biochemical and structural characterization of purified enzymes...
March 1, 2013: Antioxidants & Redox Signaling
Stephen P Kidd, Donald Jiang, Alexandra Tikhomirova, Michael P Jennings, Alastair G McEwan
BACKGROUND: adhC from Haemophilus influenzae encodes a glutathione-dependent alcohol dehydrogenase that has previously been shown to be required for protection against killing by S-nitrosoglutathione (GSNO). This group of enzymes is known in other systems to be able to utilize substrates that form adducts with glutathione, such as aldehydes. RESULTS: Here, we show that expression of adhC is maximally induced under conditions of high oxygen tension as well as specifically with glucose as a carbon source...
2012: BMC Microbiology
Daisuke Koma, Hayato Yamanaka, Kunihiko Moriyoshi, Takashi Ohmoto, Kiyofumi Sakai
Escherichia coli was metabolically engineered by expanding the shikimate pathway to generate strains capable of producing six kinds of aromatic compounds, phenyllactic acid, 4-hydroxyphenyllactic acid, phenylacetic acid, 4-hydroxyphenylacetic acid, 2-phenylethanol, and 2-(4-hydroxyphenyl)ethanol, which are used in several fields of industries including pharmaceutical, agrochemical, antibiotic, flavor industries, etc. To generate strains that produce phenyllactic acid and 4-hydroxyphenyllactic acid, the lactate dehydrogenase gene (ldhA) from Cupriavidus necator was introduced into the chromosomes of phenylalanine and tyrosine overproducers, respectively...
September 2012: Applied and Environmental Microbiology
Alastair G McEwan, Karrera Y Djoko, Nathan H Chen, Rafael L M Couñago, Stephen P Kidd, Adam J Potter, Michael P Jennings
Recognition of the diversity of transcriptional regulators of the MerR family has increased considerably over the last decade and it has been established that not all MerR-like regulators are involved in metal ion recognition. A new type of MerR-like regulator was identified in Neisseria gonorrhoeae that is distinct from metal-binding MerR proteins. This novel transcription factor, the Neisseria merR-like regulator (NmlR) is related to a large and diverse group of MerR-like regulators. A common feature of the majority of the genes encoding the nmlR-related genes is that they predicted to control the expression of adhC, which encodes a glutathione-dependent alcohol dehydrogenase...
2011: Advances in Microbial Physiology
A Michalke, A Janshoff, C Steinem, C Henke, M Sieber, H J Galla
The quantification of small molecules in aqueous solution by surface bound supramolecular host molecules is an important goal in the research field of chemo- and biosensor development. In this paper we present an attempt to quantify the interaction of different charged guest molecules with chemisorbed monothiolated β-cyclodextrin monolayers by means of impedance spectroscopy in the presence of the redox couple [Fe(CN)(6)](3)(-)/[Fe(CN)(6)](4)(-). Self-assembled monolayers of mercaptopropane-N-mono-6-deoxy-β-cyclodextrin amide (MPA-CD) on gold surfaces were formed with coverage of 99-100%...
July 1, 1999: Analytical Chemistry
Sarajane Brittis
This article is about the importance of retaining a focus on the humanity of older adults while rendering care to them through community and long-term care services. It is written from my experience as a director of a community service (Adult Day Health Care [ADHC] program), a program officer at a foundation devoted to improving health care for older Americans, a researcher who spent years conducting interviews with staff workers in long-term care services, and from the perspective of a professional and personal caregiver and friend to many elders living in the community--in their own homes and in nursing homes...
2011: Care Management Journals: Journal of Case Management ; the Journal of Long Term Home Health Care
Abiodun D Ogunniyi, Layla K Mahdi, Michael P Jennings, Alastair G McEwan, Christopher A McDevitt, Mark B Van der Hoek, Christopher J Bagley, Peter Hoffmann, Katherine A Gould, James C Paton
The importance of Mn(2+) for pneumococcal physiology and virulence has been studied extensively. However, the specific cellular role(s) for which Mn(2+) is required are yet to be fully elucidated. Here, we analyzed the effect of Mn(2+) limitation on the transcriptome and proteome of Streptococcus pneumoniae D39. This was carried out by comparing a deletion mutant lacking the solute binding protein of the high-affinity Mn(2+) transporter, pneumococcal surface antigen A (PsaA), with its isogenic wild-type counterpart...
September 2010: Journal of Bacteriology
Adam J Potter, Stephen P Kidd, Alastair G McEwan, James C Paton
The NmlR(sp) transcription factor of Streptococcus pneumoniae is shown to induce adhC (alcohol dehydrogenase) expression in the presence of both formaldehyde and methylglyoxal. nmlR(sp) and adhC mutant strains display altered and opposite aerobic growth phenotypes. The nmlR(sp) strain exhibits increased resistance to high oxygen tension, attributable to decreased H(2)O(2) production, which correlated with downregulation of carbamoyl phosphate synthase (carB). This indicates a possible role for AdhC in aldehyde metabolism and a broader role for NmlR(sp) in the regulation of carbon metabolism...
August 2010: Journal of Bacteriology
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