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Filip B J L Stillaert, Casper Sommeling, Salvatore D'Arpa, David Creytens, Koenraad Van Landuyt, Herman Depypere, Rudy Van den Broecke, Stan Monstrey, Phillip N Blondeel, Wayne A Morrison
BACKGROUND: Breast reconstruction involves the use of autologous tissues or implants. Occasionally, microsurgical reconstruction is not an option because of insufficient donor tissues. Fat grafting has become increasingly popular in breast surgery. The challenge with this technique is how to reconstruct a stable and living "scaffold" that resembles a breast. METHODS: Breast reconstruction (n = 7) was performed using intratissular expansion with serial deflation-lipofilling sessions...
September 27, 2016: Journal of Plastic, Reconstructive & Aesthetic Surgery: JPRAS
Shaun S Tan, Zhi Yang Ng, Weiqing Zhan, Warren Rozen
Autologous fat grafting is commonly utilised to reconstruct soft tissue defects caused by ageing, trauma, chronic wounds and cancer resection. The benefits of fat grafting are minimal donor site morbidity and ease of availability through liposuction or lipectomy. Nonetheless, survival and longevity of fat grafts remain poor post-engraftment. Various methods to enhance fat graft survival are currently under investigation and its stem cell constituents are of particular interest. Cell-assisted lipotransfer refers to the addition of adipose-derived stem cell (ASC) rich component of stromal vascular fraction to lipoaspirate, the results of which have proven promising...
July 2016: Journal of Cutaneous and Aesthetic Surgery
Alexandra Condé-Green, Vasanth S Kotamarti, Lauren S Sherman, Jonathan D Keith, Edward S Lee, Mark S Granick, Pranela Rameshwar
: Standard isolation of adipose stromal vascular fraction (SVF) requires the use of collagenase and is considered more than "minimally manipulated" by current good manufacturing practice requirements. Alternatively, nonenzymatic isolation methods have surfaced using physical forces to separate cells from the adipose matrix. The purpose of this study was to review the literature on the use of mechanical isolation protocols and compare the results. The implication for use as a standard procedure in practice is discussed...
September 2016: Plastic and Reconstructive Surgery. Global Open
Garrett L Metcalf, Scott R McClure, Jesse M Hostetter, Rudy F Martinez, Chong Wang
Use of mesenchymal stem cells (MSCs) found in the stromal vascular fraction (SVF) of equine adipose tissue has promising applications for regenerative therapies. The most commonly used source of equine adipose tissue is the subcutaneous tailhead. The objective of this study was to compare 3 adipose depot sites in horses and determine the viability and cellular yield, capillary density, gene expression for selected markers, and colony-forming unit fibroblasts (CFU-Fs) in adipose tissue taken from these sites...
October 2016: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
Allen Gabriel, G Patrick Maxwell, Leah Griffin, Manish C Champaneria, Mousam Parekh, David Macarios
BACKGROUND: Centrifugation (Cf) is a common method of fat processing but may be time consuming, especially when processing large volumes. OBJECTIVES: To determine the effects on fat grafting time, volume efficiency, reoperations, and complication rates of Cf vs an autologous fat processing system (Rv) that incorporates fat harvesting and processing in a single unit. METHODS: We performed a retrospective cohort study of consecutive patients who underwent autologous fat grafting during reconstructive breast surgery with Rv or Cf...
October 11, 2016: Aesthetic Surgery Journal
Yi-Chun Lin, Horng-Jyh Harn, Po-Cheng Lin, Ming-Hsi Chuang, Chun-Hung Chen, Shinn-Zong Lin, Tzyy-Wen Chiou
Human adipose-derived stem cells (ADSCs) are a promising source of autologous stem cells for personalized cell-based therapies. Culture expansion of ADSCs provides an attractive opportunity for liver cirrhosis patients. However, safety and stability issues can pose big challenges for personalized autologous stem cell products. In the present study, we addressed whether the commercial production program could provide a consistent product for liver cirrhosis therapy. We collected adipose tissue from three human donors by lipoaspirate and isolated ADSCs, which were expanded in culture to reach 1 × 10(8) cells (an approximately 1000-fold expansion) within four passages...
October 6, 2016: Cell Transplantation
Jenny Lopez, Outi Huttala, Jertta-Riina Sarkanen, Ilkka Kaartinen, Hannu Kuokkanen, Timo Ylikomi
Proper functioning wound healing strategies are sparse. Adequate vascular formation to the injured area, as well as replacement of the volume loss, is fundamental in soft tissue repair. Tissue engineering strategies have been proposed for the treatment of these injury sites. Novel cell-free substance, human adipose tissue extract (ATE), has been previously shown to induce in vitro angiogenesis and adipogenesis and in vivo soft tissue formation. This study reports the translation of ATE preparation from laboratory to the operating room (OR)...
2016: BioResearch Open Access
E Raposio, S Bonomini, F Calderazzi
INTRODUCTION: Adipose tissue represents an abundant and accessible source of adult stem cells that can differentiate into cells and tissues of mesodermal origin, including osteogenic cells. METHODS: This paper describes the procedure to obtain a 5-cm(3) saline sample, containing the adipose-derived stem cells (ASCs) pellet, starting from lipoaspirate obtained from a conventional abdominal liposuction. RESULTS: A mean of 2.5×10(6) cells is isolated for each procedure; 35% (875000) of these are CD34+/CD45- cells, which express a subset of both positive (CD10, CD13, CD44, CD59, CD73, CD90, HLAABC) and negative (CD33, CD39, CD102, CD106, CD146, HLADR) cell-associated surface antigens, characterizing them as ASCs...
September 13, 2016: Orthopaedics & Traumatology, Surgery & Research: OTSR
Patrick L Tonnard, Alexis M Verpaele
No abstract text is available yet for this article.
September 10, 2016: Plastic and Reconstructive Surgery
Takanobu Mashiko, Szu-Hsien Wu, Jingwei Feng, Koji Kanayama, Kaori Kinoshita, Ataru Sunaga, Mitsunaga Narushima, Kotaro Yoshimura
INTRODUCTION: Condensation of grafted fat has been considered a key for achieving better outcomes after fat grafting. The purpose of this study was to investigate the therapeutic potentials of two mechanical tissue micronizing procedures: squeeze and emulsification. MATERIALS AND METHODS: Human aspirated fat was centrifuged (centrifuged fat: CF) and fragmented with an automated slicer (squeezed fat: SF). Alternatively, CF was emulsified by repeated transfer between two syringes through a small-hole connecter, then separated by mesh filtration into two portions: residual tissue of emulsified fat (REF) and filtrated fluid of emulsified fat (FEF)...
September 10, 2016: Plastic and Reconstructive Surgery
Joshua J Goldman, Wei Z Wang, Xin-Hua Fang, Shelley J Williams, Richard C Baynosa
BACKGROUND: Our previous study demonstrated that lidocaine has a negative impact on adipose-derived stem cell (ASC) survival. Currently for large-volume liposuction, patients often undergo general anesthesia; therefore, lidocaine subcutaneous anesthesia is nonessential. We hypothesized that removing lidocaine from tumescent might improve stromal vascular fraction (SVF) and ASC survival from the standard tumescent with lidocaine. Ropivacaine is also a commonly used local anesthetic. The effect of ropivacaine on ASC survival was examined...
August 2016: Plastic and Reconstructive Surgery. Global Open
Anna Bajek, Natalia Gurtowska, Joanna Olkowska, Małgorzata Maj, Łukasz Kaźmierski, Magdalena Bodnar, Andrzej Marszałek, Robert Dębski, Tomasz Drewa
The objective of this study was to evaluate complex biological properties of human stem cells isolated from adipose tissue (ASCs) harvested utilizing different methods: surgical resection (R), power assisted liposuction (PAL) and laser assisted liposuction (LAL). ASCs were isolated from healthy donors, due to surgical resection, power and laser assisted liposuction. Isolated cells were characterized by their clonogenicity, proliferation rate, doubling time, multilineage differentiation and senescence potential...
September 8, 2016: Journal of Cellular Biochemistry
Tommaso Agostini, Giuseppe Spinelli, Raffella Perello, Daniele Bani, Giulia Boccalini
BACKGROUND: The success of lipotransfer strongly depends on the harvesting, processing, and placement of the lipoaspirated samples. This study was designed to assess the histomorphometric characteristics and viability of fat harvested using different techniques (wet and dry) following centrifugation, as described by Coleman. METHODS: The study enrolled 85 consecutive, nonrandomized, healthy patients from March 2010 to December 2014 (45 males and 40 females). The mean age was 40 years (range, 18-59 years), and the mean body mass index was 25...
September 2016: Journal of Craniofacial Surgery
Ilknur Keskin, Mustafa Sutcu, Hilal Eren, Mustafa Keskin
BACKGROUND: Lidocaine and epinephrine could potentially decrease adipocyte viability, but these effects have not been substantiated. The phosphorylation status of perilipin in adipocytes may be predictive of cell viability. Perilipin coats lipid droplets and restricts access of lipases; phospho-perilipin lacks this protective function. OBJECTIVES: The authors investigated the effects of tumescent solution containing lidocaine and epinephrine on the phosphorylation status of perilipin in adipocytes...
September 2, 2016: Aesthetic Surgery Journal
Jaewoo Pak, Jung Hun Lee, Kwang Seung Park, Byeong Chul Jeong, Sang Hee Lee
This clinical case series demonstrates that percutaneous injections of autologous adipose tissue-derived stem cells (ADSCs) and homogenized extracellular matrix (ECM) in the form of adipose stromal vascular fraction (SVF), along with hyaluronic acid (HA) and platelet-rich plasma (PRP) activated by calcium chloride, could regenerate cartilage-like tissue in human knee osteoarthritis (OA) patients. Autologous lipoaspirates were obtained from adipose tissue of the abdominal origin. Afterward, the lipoaspirates were minced to homogenize the ECM...
2016: BioResearch Open Access
Alexandra Condé-Green, Vasanth Kotamarti, Kevin T Nini, Philip D Wey, Naveen K Ahuja, Mark S Granick, Edward S Lee
BACKGROUND: With the increasing demand for gluteal fat augmentation, reports of fatal complications have surfaced. Therefore, the authors proposed to analyze the published techniques and compare different protocols, to identify those of potential concern. METHODS: A systematic review of the literature was performed with a search of 21 terms on the PubMed, MEDLINE, Cochrane, and Scientific Electronic Library Online databases. Nineteen articles meeting our predetermined criteria were analyzed, and data from the different steps of the procedure were classified, allowing evaluation and comparison of techniques...
September 2016: Plastic and Reconstructive Surgery
Hassan H Amhaz, Kate Buretta, Edmund H Jooste, Kelly Machovec, Jeffery R Marcus, Warwick A Ames
Peripheral intravenous cannulation in children is associated with occasional morbidity. We present a case where a large volume of blood, administered through a small peripheral cannula in the antecubital fossa, was found to have extravasated into the soft tissues, causing catastrophic vascular compromise. The expedient removal of the extravasate using a lipoaspiration cannula restored perfusion immediately to the affected limb and negated the need for surgical fasciotomies.
August 22, 2016: A & A Case Reports
Carlo Tremolada, Valeria Colombo, Carlo Ventura
In the past few years, interest in adipose tissue as an ideal source of mesenchymal stem cells (MSCs) has increased. These cells are multipotent and may differentiate in vitro into several cellular lineages, such as adipocytes, chondrocytes, osteoblasts, and myoblasts. In addition, they secrete many bioactive molecules and thus are considered "mini-drugstores." MSCs are being used increasingly for many clinical applications, such as orthopedic, plastic, and reconstructive surgery. Adipose-derived MSCs are routinely obtained enzymatically from fat lipoaspirate as SVF and/or may undergo prolonged ex vivo expansion, with significant senescence and a decrease in multipotency, leading to unsatisfactory clinical results...
2016: Current Stem Cell Reports
Valentina Ceserani, Anna Ferri, Angiola Berenzi, Anna Benetti, Emilio Ciusani, Luisa Pascucci, Cinzia Bazzucchi, Valentina Coccè, Arianna Bonomi, Augusto Pessina, Erica Ghezzi, Offer Zeira, Piero Ceccarelli, Silvia Versari, Carlo Tremolada, Giulio Alessandri
BACKGROUND: Adipose-derived mesenchymal stromal cells (Ad-MSCs) are a promising tool for advanced cell-based therapies. They are routinely obtained enzymatically from fat lipoaspirate (LP) as SVF, and may undergo prolonged ex vivo expansion, with significant senescence and decline in multipotency. Besides, these techniques have complex regulatory issues, thus incurring in the compelling requirements of GMP guidelines. Hence, availability of a minimally manipulated, autologous adipose tissue would have remarkable biomedical and clinical relevance...
2016: Vascular Cell
Ruth Tevlin, Adrian McArdle, Elizabeth Brett, Michael T Chung, Kevin Paik, Eun Young Seo, Graham G Walmsley, Christopher R Duldulao, David Atashroo, Elizabeth Zielins, Stephanie Vistnes, Charles K F Chan, Derrick C Wan, Michael T Longaker
BACKGROUND: Adipose tissue represents an abundant and easily accessible source of multipotent cells, which may serve as excellent building blocks for tissue engineering. We have developed a novel protocol for isolating adipose-derived stromal cells (ASCs) from human lipoaspirate. The aim of this study was to compare our new method for ASC isolation to a previously published standard protocol. METHODS: Human ASC isolation was performed using two methods to compare cell yield, cell viability, cell proliferation and regenerative potential...
August 15, 2016: Plastic and Reconstructive Surgery
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