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https://www.readbyqxmd.com/read/29337886/mechanical-activation-of-adipose-tissue-and-derived-mesenchymal-stem-cells-novel-anti-inflammatory-properties
#1
Stephana Carelli, Mattia Colli, Valeriano Vinci, Fabio Caviggioli, Marco Klinger, Alfredo Gorio
The adipose tissue is a source of inflammatory proteins, such as TNF, IL-6, and CXCL8. Most of their production occurs in macrophages that act as scavengers of dying adipocytes. The application of an orbital mechanical force for 6-10 min at 97 g to the adipose tissue, lipoaspirated and treated according to Coleman procedures, abolishes the expression of TNF-α and stimulates the expression of the anti-inflammatory protein TNF-stimulated gene-6 (TSG-6). This protein had protective and anti-inflammatory effects when applied to animal models of rheumatic diseases...
January 16, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29334574/improved-long-term-volume-retention-of-svf-gel-grafting-with-enhanced-angiogenesis-and-adipogenesis
#2
Yuteng Zhang, Junrong Cai, Tao Zhou, Yao Yao, Ziqing Dong, Feng Lu
BACKGROUND: The apoptosis of mature adipocytes after fat grafting can result in chronic inflammation, absorption, and fibrosis, leading to unpredictable outcomes. Selective elimination of mature adipocytes may result in better outcomes and a different underlying retention mode. We previously developed a mature-adipocyte-free product, SVF-gel, derived from lipoaspirate, which eliminates adipocytes while preserving the stromal vascular fraction. This study investigated the retention and regeneration mode of SVF-gel grafting...
January 10, 2018: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/29324934/stem-cells-therapy-the-future-in-the-management-of-systemic-sclerosis-a-case-report
#3
Jung In Song, Silvanie Volz, Maria Eirini Liodaki, Peter Mailänder, Konstantinos Kalousis
OBJECTIVE: Systemic sclerosis (SSc) is a connective tissue disorder of unknown etiology, with heterogeneous clinical manifestations and chronic and often progressive course. The diffuse cutaneous form of SSc (dcSSc) is characterized by thickening of the skin (scleroderma) and distinctive involvement of multiple internal organs. Patients with limited cutaneous SSc (lcSSc) generally have long-standing Raynaud's phenomenon before other manifestations of SSc appear. Over the last decade the Interest of adipose-derived cell therapy in regenerative medicine has increased continuously...
September 2017: Hellenic Journal of Nuclear Medicine
https://www.readbyqxmd.com/read/29316139/stem-cell-therapy-in-refractory-perineal-crohn-s-disease-long-term-follow-up
#4
Claudio Wainstein, Rodrigo Quera, Daniela Fluxá, Udo Kronberg, Alejandro Conejero, Francisco López-Köstner, Claudio Jofre, Alejandro J Zarate
AIM: To describe the long-term outcomes of adipose-mesenchymal stem cells, platelet-rich plasma, and endorectal advancement flaps in patients with Perineal Crohn's Disease. METHOD: This was a single-center, prospective, observational pilot study performed between March 2013 and December 2016. The study included adult patients diagnosed with Perianal Crohn's Disease (with complex perianal fistulas) refractory to previous surgical and/or biological treatment. Patients underwent surgical treatment in two stages...
January 6, 2018: Colorectal Disease: the Official Journal of the Association of Coloproctology of Great Britain and Ireland
https://www.readbyqxmd.com/read/29310377/fat-grafting-for-resurfacing-an-exposed-implant-in-lower-extremity-a-case-report
#5
Yun-Nan Lin, Chieh-Han Chuang, Shih-Hao Huang, Shu-Hung Huang, Tsai-Ming Lin, I-Wen Lin, Sin-Daw Lin, Yur-Ren Kuo
RATIONALE: Although numerous reconstruction protocols have been reported for lower leg trauma, those for distal leg trauma remain few. We present the case of a woman with an implant exposure wound, who was successfully treated through fat grafting, without major flap surgery. PATIENT CONCERNS: An 83-year-old woman with an exposed implant in lower extremity received reconstruction surgery once and the surgery failed. She refused additional major surgery and negative pressure wound therapy...
December 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29286436/stromal-vascular-fraction-enriched-fat-grafting-for-the-treatment-of-symptomatic-end-neuromata
#6
Simon Zimmermann, Richard M Fakin, Thomas Giesen, Pietro Giovanoli, Maurizio Calcagni
The purpose of this study was to methodically illustrate and highlight the crucial steps of stromal vascular fraction (SVF)-enriched fat grafting as a novel treatment of symptomatic end-neuromata of peripheral sensory nerves, and in this study, specifically of the superficial branch of the radial nerve (SBRN). Despite a multitude of existing treatments, persistent postoperative pain and common pain relapse are still very common, independent of the procedure assessed. The neuroma is microsurgically excised accordingly to standardized protocol...
November 23, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29234944/investigating-the-mincing-method-for-isolation-of-adipose-derived-stem-cells-from-pregnant-women-fat
#7
Yuan-Sheng Li, Pao-Jen Chen, Li-Wei Wu, Pei-Wen Chou, Li-Yi Sun, Tzyy-Wen Chiou
The success of stem cell application in regenerative medicine, usually require a stable source of stem or progenitor cells. Fat tissue represents a good source of stem cells because it is rich in stem cells and there are fewer ethical issues related to the use of such stem cells, unlike embryonic stem cells. Therefore, there has been increased interest in adipose-derived stem cells (ADSCs) for tissue engineering applications. Here, we aim to provide an easy processing method for isolating adult stem cells from human adipose tissue harvested from the subcutaneous fat of the abdominal wall during gynecologic surgery...
December 12, 2017: Cytotechnology
https://www.readbyqxmd.com/read/29205140/autologous-fat-transplantation-for-secondary-breast-reconstruction-our-experience
#8
F Simonacci, M P Grieco, N Bertozzi, E Raposio
BACKGROUND: Autologous fat transplantation is used after breast reconstruction to improve the breast profile. There are a variety of different methods used for fat harvesting, preparation, and reinjection. This study describes the specific techniques we used in this series of autologous fat transplantations in breast reconstruction patients and reports their outcomes compared with other studies in the literature. PATIENTS AND METHODS: At the University Hospital of Parma between May 2012 and December 2016, we performed 53 autologous fat transplantations for secondary breast reconstruction patients with an average age of 49 years (range: 34-65 y)...
May 2017: Il Giornale di Chirurgia
https://www.readbyqxmd.com/read/29198704/ischemic-flap-survival-improvement-by-composition-selective-fat-grafting-with-novel-adipose-tissue-derived-product-stromal-vascular-fraction-gel
#9
Pan Zhang, Jingwei Feng, Yunjun Liao, Junrong Cai, Tao Zhou, Mingliang Sun, Jianhua Gao, Kai Gao
BACKGROUND: Flap necrosis due to insufficient blood supply is a common postoperative complication in random pattern flaps. Stem cell therapies have emerged as promising biologics for tissue ischemia. A novel fat derived product, stromal vascular fraction gel (SVF-gel), can be prepared with lipoaspirate through simple mechanical processing, removing only the lipid content. SVF-gel enriches adipose-derived stem cells and potentially beneficial for flap necrosis. METHODS: Nude mice ischemic flaps were treated with human SVF-gel, stromal vascular fraction (SVF) cell suspension or saline (n = 10)...
November 30, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29188051/the-science-behind-autologous-fat-grafting
#10
REVIEW
Elisa Bellini, Michele P Grieco, Edoardo Raposio
Introduction: Adipose grafting has undergone significant changes over time. Many different techniques have been followed by trying to improve the quality of the lipoaspirate and the survival of the fat graft after implantation. Material and methods: The purpose of this review is to analyse the historical evolution of the surgical harvesting and implant technique, describing the changes that have brought significant improvements, revolutionizing the aesthetic and functional results obtainable...
December 2017: Annals of Medicine and Surgery
https://www.readbyqxmd.com/read/29140567/human-adipose-derived-stromal-cell-isolation-methods-and-use-in-osteogenic-and-adipogenic-in-vivo-applications
#11
Elizabeth Brett, Ruth Tevlin, Adrian McArdle, Eun Young Seo, Charles K F Chan, Derrick C Wan, Michael T Longaker
Adipose tissue represents an abundant and easily accessible source of multipotent cells, which may serve as excellent building blocks for tissue engineering. This article presents a newly described protocol for isolating adipose-derived stromal cells (ASCs) from human lipoaspirate, compared to the standard protocol for harvesting ASCs established in 2001. Human ASC isolation is performed using two methods, and resultant cells are compared through cell yield, cell viability, cell proliferation and regenerative potential...
November 15, 2017: Current Protocols in Stem Cell Biology
https://www.readbyqxmd.com/read/29102952/comparison-of-the-viability-and-yield-of-adipose-derived-stem-cells-ascs-from-different-donor-areas
#12
Anastasios Tsekouras, Dimitrios Mantas, Diamantis I Tsilimigras, Demetrios Moris, Michael Kontos, George C Zografos
BACKGROUND: Adipose tissue contains variable amounts of stem cells that have recently attracted increased interest due to their strong proliferative and differentiation capacity. However, significant heterogeneity exists in terms of stem cell yield and viability profile among individuals as well as different donor areas. MATERIALS AND METHODS: Between June 2016 and July 2016, forty (40) women underwent outpatient cosmetic liposuction procedures conferring a total of 53 lipoaspirates; inner thigh (n=13), outer thigh (n=9), abdomen (n=9), waist (n=16) and inner knee (n=6)...
November 2017: In Vivo
https://www.readbyqxmd.com/read/29068969/positive-effect-of-incubated-adipose-derived-mesenchymal-stem-cells-on-microfat-graft-survival
#13
Jong Woo Choi, Sung-Chan Kim, Eun-Jung Park, Ju-A Lee, Woo Shik Jeong
Autologous fat grafting is commonly used for soft tissue augmentation, but its unpredictably high resorption rate remains a major limitation. Although adipose-derived mesenchymal stem cells (ASCs) are an attractive candidate for enhancing graft retention, their poor posttransplantation viability limits their application. The authors aimed to evaluate the effect of incubated ASCs on microfat graft survival in an immunocompromised mouse model. Lipoaspirates for microfat injection were collected from the wasted lower abdominal adipose tissues of 5 patients who had undergone breast reconstructive surgery with an abdominal flap...
October 24, 2017: Journal of Craniofacial Surgery
https://www.readbyqxmd.com/read/29068928/the-effect-of-processing-technique-on-fat-graft-survival
#14
COMPARATIVE STUDY
Orlando Canizares, Jennifer E Thomson, Robert J Allen, Edward H Davidson, John P Tutela, Pierre B Saadeh, Stephen M Warren, Alexes Hazen
BACKGROUND: Wide variations in fat graft survival have been reported. The authors hypothesize that treating the adipose tissue on Telfa gauze creates a processed lipoaspirate with a more functional adipokine profile that improves fat graft survival. METHODS: Suction-assisted lipoaspirate was harvested from humans and was either processed by centrifugation, rolled on Telfa gauze, or left unprocessed. Progenitor cell populations were quantified and characterized by flow cytometry...
November 2017: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/29051810/controlled-dexamethasone-delivery-via-double-walled-microspheres-to-enhance-long-term-adipose-tissue-retention
#15
Arta Kelmendi-Doko, J Peter Rubin, Katarina Klett, Christopher Mahoney, Sheri Wang, Kacey G Marra
Current materials used for adipose tissue reconstruction have critical shortcomings such as suboptimal volume retention, donor-site morbidity, and poor biocompatibility. The aim of this study was to examine a controlled delivery system of dexamethasone to generate stable adipose tissue when mixed with disaggregated human fat in an athymic mouse model for 6 months. The hypothesis that the continued release of dexamethasone from polymeric microspheres would enhance both adipogenesis and angiogenesis more significantly when compared to the single-walled microsphere model, resulting in long-term adipose volume retention, was tested...
January 2017: Journal of Tissue Engineering
https://www.readbyqxmd.com/read/29027984/the-effect-of-intra-articular-injection-of-autologous-microfragmented-fat-tissue-on-proteoglycan-synthesis-in-patients-with-knee-osteoarthritis
#16
Damir Hudetz, Igor Borić, Eduard Rod, Željko Jeleč, Andrej Radić, Trpimir Vrdoljak, Andrea Skelin, Gordan Lauc, Irena Trbojević-Akmačić, Mihovil Plečko, Ozren Polašek, Dragan Primorac
Osteoarthritis (OA) is one of the leading musculoskeletal disorders in the adult population. It is associated with cartilage damage triggered by the deterioration of the extracellular matrix tissue. The present study explores the effect of intra-articular injection of autologous microfragmented adipose tissue to host chondrocytes and cartilage proteoglycans in patients with knee OA. A prospective, non-randomized, interventional, single-center, open-label clinical trial was conducted from January 2016 to April 2017...
October 13, 2017: Genes
https://www.readbyqxmd.com/read/29025229/mechanical-supplementation-with-the-stromal-vascular-fraction-yields-improved-volume-retention-in-facial-lipotransfer-a-1-year-comparative-study
#17
Natale Ferreira Gontijo-de-Amorim, Luiz Charles-de-Sá, Gino Rigotti
Background: Unpredictable volume maintenance in the long term is a major limitation of autologous fat grafting. Objectives: The authors compared results of autologous lipotransfer to the face with or without enrichment of fat with the stromal vascular fraction (SVF). Methods: Thirty patients with asymmetric depletion of facial volume were included in a prospective study. Patients were randomly assigned to undergo a single session of autologous fat transfer with washed adipose tissue (control group) or with washed adipose tissue combined with the pellet of centrifuged lipoaspirate, which contained the SVF (enriched group)...
October 1, 2017: Aesthetic Surgery Journal
https://www.readbyqxmd.com/read/29025217/use-of-lipotransfer-in-scleroderma
#18
Michelle F Griffin, Aurora Almadori, Peter E Butler
Lipotransfer for soft tissue filling is a well-established plastic and aesthetic surgical technique. Elective fat grafting is considered a safe and well-tolerated procedure. Coleman and others have reported that fat grafting may have tissue regenerative properties and not only serve as a soft tissue filler. There have been reports from our group and others that it may improve fibrosis secondary to many different pathological aetiologies including scleroderma, burn injury, lichen sclerosis, graft vs host disease, and radiation...
July 1, 2017: Aesthetic Surgery Journal
https://www.readbyqxmd.com/read/29025216/assisted-salvage-of-ischemic-fasciocutaneous-flap-using-adipose-derived-mesenchymal-stem-cells-in-situ-revascularization
#19
Michael H Carstens, Mauricio Mendieta, Cecilia Pérez, Esperanza Villareal, Rodolfo Garcia
Adipose-derived mesenchymal stem cells (ASCs) have been shown to produce vascular endothelial growth factor (VEGF) and can increase perfusion in patients with critical limb ischemia. We will show that this concept can be applied to augment blood flow in zones of flap ischemia. We presented a case study of a 26-year-old man with a complex hand injury covered by a reverse radial perforator fasciocutaneous flap, which developed ischemic necrosis and was treated by debridement, transplantation of ASCs to enhance vascular support, and saline dressings...
July 1, 2017: Aesthetic Surgery Journal
https://www.readbyqxmd.com/read/29025212/use-of-freshly-isolated-human-adipose-stromal-cells-for-clinical-applications
#20
Ryan A Lockhart, Joel A Aronowitz, Severiano Dos-Anjos Vilaboa
The clinical use of adipose-derived cells is being explored very actively around the world for various human diseases. Adipose tissue is an abundant tissue source that can be easily harvested using liposuction. Human lipoaspirates contain a significant amount of mesenchymal stromal cells, as well as other progenitors and terminally differentiated cell types. This review covers the isolation of adipose stromal vascular fraction (SVF), the quality control and safety analysis of freshly isolated cell suspensions...
July 1, 2017: Aesthetic Surgery Journal
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