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Lipoaspirate

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https://www.readbyqxmd.com/read/29778249/a-preoperative-cotininury-test-for-abdominoplasty-reduces-peri-operative-complications
#1
F Delaunay, D Coquerel-Beghin, G Magalon, S R Cohen, D Casanova, J Niddam, P-Y Milliez, C Peillon, V Delpierre, I Auquit-Auckbur
BACKGROUND: Smoking induces complications in plastic surgery, in particular wound healing delays. Despite a 4-weeks' abstinence asking before and after surgery, some patients denied or hid their consumption. The aim of this study was to evaluate the effectiveness of a cotininury detection test in terms of improvement in outcomes after an abdominoplasty. MATERIAL AND METHODS: This retrospective cohort study included patients who underwent an abdominoplasty with umbilical transposition and lipoaspiration...
May 16, 2018: Annales de Chirurgie Plastique et Esthétique
https://www.readbyqxmd.com/read/29775452/the-effects-of-a-pulsed-electromagnetic-field-on-the-proliferation-and-osteogenic-differentiation-of-human-adipose-derived-stem-cells
#2
Yukun Yin, Ping Chen, Qiang Yu, Yan Peng, ZeHao Zhu, Jing Tian
BACKGROUND A low frequency pulsed electromagnetic field (PEMF) has been confirmed to play an important role in promoting the osteogenic differentiation of human bone marrow stem cells (BMSCs). Adipose-derived stem cells (ASCs) possess some attractive characteristics for clinical application compared to BMSCs, such as abundant stem cells from lipoaspirates, faster growth, less discomfort and morbidity during surgery. ASCs can become adipocytes, osteoblasts, chondrocytes, myocytes, neurocytes, and other cell types...
May 18, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29764513/cellular-therapy-with-human-autologous-adipose-derived-adult-cells-of-stromal-vascular-fraction-for-alopecia-areata
#3
Rami Anderi, Nehman Makdissy, Albert Azar, Francine Rizk, Aline Hamade
BACKGROUND: Most common forms of hair loss (alopecia) are caused by aberrant hair follicle cycling and changes in hair follicle morphology. However, current treatments for alopecia do not specifically target these processes. Adipose-derived stromal vascular cells (ADSVCs) that can be harvested from fat cells are one of the latest breakthroughs in the aesthetic field. The potential use of stem cell-based therapies (SCBT) for the repair and regeneration of various tissues and organs offers a paradigm shift that may provide alternative therapeutic solutions, which can be applied to prevent hair loss...
May 15, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29751821/improved-gmp-compliant-approach-to-manipulate-lipoaspirates-to-cryopreserve-stromal-vascular-fraction-and-to-expand-adipose-stem-cells-in-xeno-free-media
#4
Francesco Agostini, Francesca Maria Rossi, Donatella Aldinucci, Monica Battiston, Elisabetta Lombardi, Stefania Zanolin, Samuele Massarut, Pier Camillo Parodi, Alessandro Da Ponte, Giovanni Tessitori, Barbara Pivetta, Cristina Durante, Mario Mazzucato
BACKGROUND: The stromal vascular fraction (SVF) derived from adipose tissue contains adipose-derived stromal/stem cells (ASC) and can be used for regenerative applications. Thus, a validated protocol for SVF isolation, freezing, and thawing is required to manage product administration. To comply with Good Manufacturing Practice (GMP), fetal bovine serum (FBS), used to expand ASC in vitro, could be replaced by growth factors from platelet concentrates. METHODS: Throughout each protocol, GMP-compliant reagents and devices were used...
May 11, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29750320/the-m-y-axilloplasty-after-massive-weight-loss-analysis-of-159-consecutive-patients
#5
David Boccara, Arnaud Petit, Christophe Reinbold, Marc Chaouat, Maurice Mimoun, Kevin Serror
BACKGROUND: Brachioplasties often culminate in unsightly scars that are a source of disappointment to patients. We aimed to evaluate the results of M-Y axilloplasty following massive weight loss. PATIENTS: We performed a retrospective assessment of our technique for brachioplasty with an M-Y axilloplasty in 159 female patients after massive weight loss. This retrospective study covered a study period of 10 years. METHOD: After substantial lipoaspiration, the incision is placed on the internal side of the arm, with an M-shaped axilloplasty...
May 10, 2018: Aesthetic Plastic Surgery
https://www.readbyqxmd.com/read/29722790/utility-of-adipocyte-fractions-in-fat-grafting-in-an-athymic-rat-model
#6
Yucel Akgul, Ryan Constantine, Mason Bartels, Philipp Scherer, Kathryn Davis, Jeffrey M Kenkel
Background: Multiple processing and handling methods of autologous fat yields to variations in graft retention and viability, which results in unpredictable clinical outcomes. Objective: This study aims to understand the skin effects of fat graft preparations that contain a varying ratio of free-lipid and stem-cell-bearing stromal vascular fractions (SVF). Methods: Lipoaspirates from consenting patients were processed into emulsified fat and then SVF and adipocyte fractions (free-lipid)...
May 2, 2018: Aesthetic Surgery Journal
https://www.readbyqxmd.com/read/29697607/use-of-tranexamic-acid-to-reduce-blood-loss-in-liposuction
#7
Alvaro Luiz Cansancao, Alexandra Condé-Green, Joshua A David, Bethania Cansancao, Rafael A Vidigal
BACKGROUND: The use of tranexamic acid for blood loss prevention has gained popularity in many specialties, including plastic surgery. However, its use in liposuction has not been studied. The authors present a prospective, double-blind, nonrandomized study evaluating the efficacy of tranexamic acid in reducing perioperative blood loss during liposuction. METHODS: Twenty women undergoing liposuction were divided into two cohorts. Group 1 (n = 10) received a standard dose of 10 mg/kg of tranexamic acid intravenously in the preoperative and postoperative periods, whereas group 2 (n = 10) received a placebo...
May 2018: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/29687388/isolation-of-human-adipose-derived-stem-cells-from-lipoaspirates
#8
Jie Li, J Lowry Curley, Z Elizabeth Floyd, Xiying Wu, Yuan Di C Halvorsen, Jeffrey M Gimble
Adipose tissue is as an abundant and accessible source of stem cells with multipotent properties suitable for tissue engineering and regenerative medical applications. Here, we describe methods from our own laboratory and the literature for the isolation and expansion of adipose-derived stem cells (ASCs). We present a large scale procedure suitable for processing >100 mL volumes of lipoaspirate tissue specimens by collagenase digestion and a related procedure suitable for processing adipose tissue aspirates without digestion...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29672782/-comparison-of-the-regenerative-cytokine-profile-of-adipose-derived-stromal-cells-ascs-harvested-via-abdominoplasty-suction-assisted-liposuction-sal-and-ultrasound-assisted-liposuction-ual
#9
Matthias Michael Aitzetmüller, Hans-Günther Machens, Arndt F Schilling, Dominik Duscher
INTRODUCTION: Mesenchymal stem cells are already used in numerous areas of medicine. In the field of plastic surgery, the main focus of recent research has been to utilise adipose-derived stromal cells (ASCs), mainly harvested via liposuction. The ability to release regenerative cytokines is supposed to be the key mechanism for the therapeutic efficacy of ASCs. There are currently a variety of methods and devices in clinical use for harvesting lipoaspirate and consequently ASCs. For most of these devices, the effect on the regenerative cytokine profile of ASCs is unknown...
April 2018: Handchirurgie, Mikrochirurgie, Plastische Chirurgie
https://www.readbyqxmd.com/read/29663271/regenerative-potential-and-inflammation-induced-secretion-profile-of-human-adipose-derived-stromal-vascular-cells-are-influenced-by-donor-variability-and-prior-breast-cancer-diagnosis
#10
Adrienne M Parsons, Deborah M Ciombor, Paul Y Liu, Eric M Darling
Adipose tissue contains a heterogeneous population of stromal vascular fraction (SVF) cells that work synergistically with resident cell types to enhance tissue healing. Ease of access and processing paired with therapeutic promise make SVF cells an attractive option for autologous applications in regenerative medicine. However, inherent variability in SVF cell therapeutic potential from one patient to another hinders prognosis determination for any one person. This study investigated the regenerative properties and inflammation responses of thirteen, medically diverse human donors...
April 16, 2018: Stem Cell Reviews
https://www.readbyqxmd.com/read/29658381/hydrogen-peroxide-a-potent-inducer-of-differentiation-of-human-adipose-derived-stem-cells-into-chondrocytes
#11
Farjam Goudarzi, Adel Mohammadalipour, Majid Bahabadi, Mohammad Taghi, Goodarzi, Arash Sarveazad, Iraj Khodadadi
Common protocols for chondrogenic differentiation of adipose-derived mesenchymal stem cells (ADSCs) are generally expensive and time-consuming and so far, have not successfully recreated pure chondrocytes. We hypothesize that a low level of H2 O2 may induce differentiation of ADSCs into chondrocytes in a shorter incubation time and relatively lower cost. Therefore, this study aimed to comparatively investigate the effectiveness of H2 O2 -containing or free medium in the induction of ADSCs to chondrocytes. ADSCs were isolated from the lipoaspirate of four healthy females and evaluated by immunophenotyping for their CD90, CD73, CD44, CD34, and CD45 cell surface markers...
April 15, 2018: Free Radical Research
https://www.readbyqxmd.com/read/29652075/impact-of-tgf-%C3%AE-family-related-growth-factors-on-chondrogenic-differentiation-of-adipose-derived-stem-cells-isolated-from-lipoaspirates-and-infrapatellar-fat-pads-of-osteoarthritic-patients
#12
E López-Ruiz, G Jiménez, W Kwiatkowski, E Montañez, F Arrebola, E Carrillo, S Choe, J A Marchal, M Perán
The success of cell-based approaches for the treatment of cartilage defects requires an optimal autologous cell source with chondrogenic differentiation ability that maintains its differentiated properties and stability following implantation. The objective of this study was to compare the chondrogenic capacity of mesenchymal stem cells (MSCs) isolated from lipoaspirates (ASCs) and the infrapatellar fat pad (IFPSCs) of osteoarthritic patients and treated with transforming growth factor (TGF)-β family-related growth factors...
April 13, 2018: European Cells & Materials
https://www.readbyqxmd.com/read/29651395/fat-grafting-technique-a-paradigm-shift-in-the-treatment-of-tuberous-breast
#13
Claudiox Claudio Silva-Vergara, Joan Fontdevila, Omar Weshahy
BACKGROUND: The tuberous breast syndrome is a condition that affects young women and can bring on serious disorders of self-esteem. There are numerous surgical techniques to correct this condition, but most of them include the use of breast implants to replace lack of volume. Nowadays, patients are increasingly becoming more demanding to get a definitive solution with minimal aesthetic sequelae. METHODS: We present a series of 11 patients with tuberous breast deformities treated with lipofilling technique...
January 2018: World Journal of Plastic Surgery
https://www.readbyqxmd.com/read/29649060/liposuction-aspirate-fluid-ascs-injection-and-secondary-healing-in-fingertip-injury-a-pilot-study
#14
M Tarallo, P Fino, D Ribuffo, D Casella, M Toscani, C Spalvieri, W Lattanzi, G Di Taranto
BACKGROUND: Although fingertip injuries account for a high proportion of trauma patients, the correct surgical approach is still debated. This prospective study compares the traditional conservative approach and a new treatment based on the injection of liposuction aspirate fluid (LAF). METHODS: 40 consecutive patients with a fingertip injury were dichotomized into group A (control group: conservative approach) and B (treatment group). Group B underwent liposuction, followed by filtration of the lipoaspirate in a closed device (MyStemEvo®kit), allowing the nonenzymatic separation of LAF, which was then injected at injured site...
March 29, 2018: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/29649059/enhancement-of-progenitor-cells-by-two-step-centrifugation-of-emulsified-lipoaspirates
#15
Norbert Pallua, Justinas Grasys, Bong-Sung Kim
BACKGROUND: Adipose-derived stem cells (ASCs), endothelial progenitor cells (EPCs) and soluble factors jointly contribute to the regenerative effect of fat grafts. The nanofat grafting technique is a simple method to mechanically emulsify the lipoaspirate and increase the ASC yield in the lipoaspirate. In the present study, we evaluated our extended nanofat grafting method that includes two additional centrifugation steps and results in a lipoaspirate of low volume that we termed "lipoconcentrate"...
March 29, 2018: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/29606893/first-in-man-intravenous-implantation-of-stromal-vascular-fraction-in-psoriasis-a-case-study
#16
Kristin Comella, Michelle Parlo, Rosemary Daly, Kellie Dominessy
Background: Stromal vascular fraction (SVF) is a mixture of adipose-derived stem cells/mesenchymal stem cells, endothelial/progenitors, pericytes, fibroblasts, and other cells obtained from fat tissue. A small sample of fat or adipose tissue can be obtained under local anesthesia using a cannula. After an enzymatic digestion and centrifugation, the adipocytes (fat cells) are removed to obtain an SVF. Here, we describe the rationale and, to our knowledge, the first clinical implementation of SVF intravenously in a patient with severe psoriasis...
2018: International Medical Case Reports Journal
https://www.readbyqxmd.com/read/29595724/an-update-on-the-safety-and-efficacy-of-outpatient-plastic-surgery-a-review-of-26-032-consecutive-cases
#17
Rod J Rohrich, Bernardino M Mendez, Paul N Afrooz
BACKGROUND: Outpatient surgery offers many advantages, including cost-containment, privacy, and convenience. However, patient safety must take precedence over these benefits. Limited well-designed studies exist in the plastic surgery literature on patient safety in the outpatient setting, particularly those that identify risk factors for adverse outcomes. METHODS: A retrospective review was performed on 26,032 consecutive cases completed by board-certified plastic surgeons at an accredited outpatient surgical center between 1995 and 2017...
April 2018: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/29531535/a-nonenzymatic-and-automated-closed-cycle-process-for-the-isolation-of-mesenchymal-stromal-cells-in-drug-delivery-applications
#18
Valentina Coccè, Anna Brini, Aldo Bruno Giannì, Valeria Sordi, Angiola Berenzi, Giulio Alessandri, Carlo Tremolada, Silvia Versari, Antonio Bosetto, Augusto Pessina
The adipose tissue is a good source of mesenchymal stromal cells that requires minimally invasive isolation procedures. To ensure reproducibility, efficacy, and safety for clinical uses, these procedures have to be in compliant with good manufacturing practices. Techniques for harvesting and processing human adipose tissue have rapidly evolved in the last years, and Lipogems® represents an innovative approach to obtain microfragmented adipose tissue in a short time, without expansion and/or enzymatic treatment...
2018: Stem Cells International
https://www.readbyqxmd.com/read/29511946/adipose-tissue-extract-shows-potential-for-wound-healing-in-vitro-proliferation-and-migration-of-cell-types-contributing-to-wound-healing-in-the-presence-of-adipose-tissue-preparation-and-platelet-rich-plasma
#19
Jenny F López, Jertta-Riina Sarkanen, Outi Huttala, Ilkka S Kaartinen, Hannu O Kuokkanen, Timo Ylikomi
Growth factors are the key elements in wound healing signaling for cell migration, differentiation and proliferation. Platelet-rich plasma (PRP), one of the most studied sources of growth factors, has demonstrated to promote wound healing in vitro and in vivo. Adipose tissue is an alternative source of growth factors. Through a simple lipoaspirate method, adipose derived growth factor-rich preparation (adipose tissue extract; ATE) can be obtained. The authors set out to compare the effects of these two growth factor sources in cell proliferation and migration (scratch) assays of keratinocyte, fibroblast, endothelial and adipose derived stem cells...
March 6, 2018: Cytotechnology
https://www.readbyqxmd.com/read/29504091/in-vitro-modeling-of-co-transplantation-of-multipotent-stromal-mesenchymal-cells-from-orbital-fat-pad-and-lipoaspirate-of-human-subcutaneous-adipose-tissue-in-organ-culture-in-collagen-gel
#20
S A Borzenok, D S Afanas'eva, M B Gushchina, D S Ostrovskii, S P Domogatsky, E O Osidak
The interplay of multipotent stromal cells derived from the orbital fat pads and cells of the lipoaspirate from the subcutaneous adipose tissue was studied using in vitro co-transplantation model in an organ culture in a collagen gel. Microscopy findings and intensity of apoptosis and cell proliferation in cultures of lipoaspirate with and without multipotent stromal cells showed that the cells maintained their viability, proliferation capacity, and cytokine secretion activity. Higher proliferatitive activity of cells in cocultures promotes renewal of fat transplant cells and can help to maintain its stable volume in delayed terms after transplantation...
March 2018: Bulletin of Experimental Biology and Medicine
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