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Lipoaspirate

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https://www.readbyqxmd.com/read/28806292/-optimization-and-standardization-of-the-immunodeficient-mouse-model-for-assessing-fat-grafting-outcomes
#1
Lauren E Kokai, Taraneh L Jones, Russell Silowash, Brian Theisen, Gabriella DiBernardo, Andrew Lu, Bowen Yi, Kacey G Marra, J Peter Rubin
BACKGROUND: Animal models are often used to assess interventions that might improve fat grafting outcomes, however, there is great variability in the models. We sought to determine the predictive value of the immunocompromised mouse model for fat grafting so that experiments could be standardized and optimized. METHODS: Human lipoaspirate injections at different volumes and time points were assessed in a nude mouse model and compared with control injections of non-viable fat...
August 3, 2017: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/28750144/therapy-of-ulcus-cruris-of-venous-and-mixed-venous-arterial-origin-with-autologous-adult-native-progenitor-cells-from-subcutaneous-adipose-tissue-a-prospective-clinical-pilot-study
#2
A Konstantinow, A Arnold, K Djabali, W Kempf, J Gutermuth, T Fischer, T Biedermann
BACKGROUND: The stromal vascular fraction (SVF) of adipose tissue consists of cellular subpopulations with distinct regenerative potential. OBJECTIVE: To investigate the regenerative capacities of autologous SVF cells in the treatment of chronic leg ulcers of venous (VLU) and arterial-venous (AVLU) origin. METHODS: Multimorbid ulcer patients received a singular topical treatment with 9-15x10(6) SVF cells, separated from abdominal lipoaspirates by digestion with collagenase and dispase and applied immediately after isolation...
July 27, 2017: Journal of the European Academy of Dermatology and Venereology: JEADV
https://www.readbyqxmd.com/read/28737560/influence-of-large-volume-liposuction-on-metabolic-and-cardiovascular-health-a-systematic-review
#3
Alexander M Sailon, J Roscoe Wasserburg, Rochelle R Kling, Christina M Pasick, Peter J Taub
BACKGROUND: Evidence suggesting that adipose tissue is a metabolically active tissue has generated debate on the effects of large-volume liposuction (LVL) on metabolic and cardiovascular health. Given the inconsistency in the literature, the authors performed a systematic review to identify available evidence in order to elucidate the potential impact of LVL on metabolic markers and cardiovascular risk factors. METHODS: A PubMed search using relevant keywords was conducted...
July 22, 2017: Annals of Plastic Surgery
https://www.readbyqxmd.com/read/28736612/adipose-derived-stem-cells-comparison-between-two-methods-of-isolation-for-clinical-applications
#4
Edoardo Raposio, Francesco Simonacci, Rosario E Perrotta
BACKGROUND: Adipose-derived stem cells are recognized as being an effective mesenchymal stem cell population with enormous potential in different fields of regenerative medicine and stem cell therapy. Although there is unanimous agreement on the harvesting procedure for adipose tissue, there are various protocols for adipose-derived stem cell isolation. The aim of this study was compare two methods of adipose-derived stem cells (ASCs) isolation, one based on a mechanical + enzymatic (ME) procedure and the other one exclusively mechanical (MC), in order to determine which one was superior to the other in accordance with current European and US legislation...
August 2017: Annals of Medicine and Surgery
https://www.readbyqxmd.com/read/28702187/procedure-applications-and-outcomes-of-autologous-fat-grafting
#5
REVIEW
Francesco Simonacci, Nicolò Bertozzi, Michele Pio Grieco, Eugenio Grignaffini, Edoardo Raposio
OBJECTIVE: To systematically review the procedure, applications, and outcomes of autologous fat grafting, a promising technique with various clinical applications. PATIENTS AND METHODS: Literature review of publications concerning autologous fat grafting. RESULTS: Since its introduction, lipofilling has become increasingly popular; however, its results are variable and unpredictable. Several modifications have been made to the procedures of fat harvesting, processing, and injecting...
August 2017: Annals of Medicine and Surgery
https://www.readbyqxmd.com/read/28657470/point-of-care-adipose-derived-stromal-vascular-fraction-cell-isolation-and-expanded-polytetrafluoroethylene-graft-sodding
#6
Stuart K Williams, Marvin E Morris, Paul E Kosnik, Kevin D Lye, Gary D Gentzkow, Charles B Ross, Amit J Dwevidi, Leigh B Kleinert
Adipose-derived stromal vascular fraction (SVF) cell populations are being evaluated for numerous clinical applications. The current study evaluated a point-of-care technology, the Tissue Genesis "TGI 1000" Cell Isolation System™, to perform an automated isolation of adipose-derived SVF cells to be used in the fabrication of a tissue-engineered vascular graft in the operating room. A total of seven patients were enrolled in this study and received femoral to tibial expanded polytetrafluoroethylene bypass grafts to treat peripheral arterial disease...
August 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28639068/what-about-the-rheological-properties-of%C3%A2-prp-microfat-mixtures-in-fat-grafting-procedure
#7
R Ghazouane, B Bertrand, C Philandrianos, J Veran, M Abellan, P Francois, M Velier, C Orneto, P Piccerelle, J Magalon
BACKGROUND: Fat grafting has emerged as a reference procedure in daily plastic surgery practice. Unpredictable fat resorption is the main clinical problem. For this purpose, the addition of PRP to enhance fat revascularization is now an easy and popular procedure. However, no consensus exists regarding the respective volume of fat and PRP used to obtain the ideal mixture. This study investigated the rheological properties of microfat mixed with different proportions of PRP. Results obtained were compared with commercialized hyaluronic acid fillers...
June 21, 2017: Aesthetic Plastic Surgery
https://www.readbyqxmd.com/read/28629466/intra-articular-injection-in-the-knee-of-adipose-derived-stromal-cells-stromal-vascular-fraction-and-platelet-rich-plasma-for-osteoarthritis
#8
Himanshu Bansal, Kristin Comella, Jerry Leon, Poonam Verma, Diwaker Agrawal, Prasad Koka, Thomas Ichim
BACKGROUND: Stromal vascular fraction (SVF) can easily be obtained from a mini-lipoaspirate procedure of fat tissue and platelet rich plasma (PRP) can be obtained from peripheral blood. We evaluated the safety and preliminary efficacy of administering SVF and PRP intra-articularly into patients with osteoarthritis grade 1 and 2. METHODS: A total of ten patients underwent a local tumescent liposuction procedure to remove approximately 100 ml of fat tissue from the abdomen...
June 19, 2017: Journal of Translational Medicine
https://www.readbyqxmd.com/read/28625922/macrophage-infiltration-regulates-the-adipose-ecm-reconstruction-and-the-fibrosis-process-after-fat-grafting
#9
Junrong Cai, Bin Li, Kaiyang Liu, Guanghui Li, Feng Lu
BACKGROUND: Fat grafting experiences a regeneration process from free lipoaspirate to intact adipose tissue. The adipose extracellular matrixes (ECM) provide the structure and biochemical support for surrounding cells; inflammatory cells, like macrophages, regulate the process. Our hypothesis states that transferred fat undergoes ECM remodeling after fat grafting and this process is regulated by macrophage infiltration. METHODS: Lipoaspirate was injected subcutaneously into the back of nude mice...
August 19, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28615811/adipokine-concentrations-in-lipoaspirates-may-have-a-role-in-wound-healing
#10
Christian Herold, Stefan Engeli, Bibiana Beckmann, Peter M Vogt, Hans-Oliver Rennekampff
OBJECTIVES: In addition to its use as a volume filler, fat grafting may have a potential role in wound healing based on the concentration of growth factors in the lipoaspirate. In this study, we compare the quantitative and qualitative concentration of the various growth factors and adipokines using the Shippert or the Coleman techniques to prepare the lipoaspirate. METHODS: We measured leptin, adiponectin and the growth factors, i.e., acidic fibroblast growth factor (aFGF), basic FGF (bFGF), keratinocyte growth factor (KGF), bone morphogenetic protein-2 (BMP-2) and vascular endothelial growth factor (VEGF) by ELISA in solid and liquid fractions obtained with both techniques in human fat obtained with Coleman technique and Shippert technique...
January 2017: Indian Journal of Plastic Surgery: Official Publication of the Association of Plastic Surgeons of India
https://www.readbyqxmd.com/read/28599842/composite-breast-reconstruction-implant-based-breast-reconstruction-with%C3%A2-adjunctive-lipofilling
#11
C E Sommeling, K Van Landuyt, H Depypere, R Van den Broecke, S Monstrey, P N Blondeel, W A Morrison, F B Stillaert
INTRODUCTION: Options for breast reconstructions enclose autologous tissue transfers or implants. Fat grafting is gaining more interest in this specific field of breast surgery. This study concentrates on the technique and aesthetic results of breast reconstruction with fat grafts combined with implants, in women who have undergone total mastectomy. METHODS: Breast reconstructions (n = 23) was performed using a protocol of intratissular expansion with serial deflation-lipofilling...
May 22, 2017: Journal of Plastic, Reconstructive & Aesthetic Surgery: JPRAS
https://www.readbyqxmd.com/read/28574947/dynamic-rheology-for-the-prediction-of-surgical-outcomes-in-autologous-fat-grafting
#12
Anna Luan, Elizabeth R Zielins, Taylor Wearda, David A Atashroo, Charles P Blackshear, Jordan Raphel, Elizabeth A Brett, John Flacco, Michael C Alyono, Arash Momeni, Sarah Heilshorn, Michael T Longaker, Derrick C Wan
BACKGROUND: Due to the abundance and biocompatibility of fat, lipotransfer has become an attractive method for treating soft tissue deficits. However, it is limited by unpredictable graft survival and retention. Currently, little is known about the viscoelastic properties of fat after various injection methods. Here, we assess the effects of cannula diameter, length, and shape on the viscoelastic properties, structure, and retention of fat. METHODS: Human lipoaspirate was harvested using suction-assisted liposuction and prepared for grafting...
May 31, 2017: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/28545495/the-impact-of-human-adipose-tissue-derived-stem-cells-on-breast-cancer-cells-implications-for-cell-assisted-lipotransfers-in-breast-reconstruction
#13
Eva Koellensperger, Lilly-Claire Bonnert, Inka Zoernig, Frederik Marmé, Stefanie Sandmann, Günter Germann, Felix Gramley, Uwe Leimer
BACKGROUND: In this study we evaluated the interactions of human adipose tissue-derived stem cells (ADSCs) and different human breast cancer cell lines (BRCAs) with regard to the safety of cell-assisted lipotransfers for breast reconstruction and a thereby unintended co-localization of ADSCs and BRCAs. METHODS: ADSCs were co-cultured with five different human BRCAs (MCF-7, MDA-MB-231, SK-BR-3, ZR-75-30, and EVSA-T) and primary BRCAs from one patient in a transwell system, and cell-cell-interactions were analyzed by assessing doubling time, migration and invasion, angiogenesis, quantitative real-time polymerase chain reaction (PCR) of more than 300 tumor-associated genes, and multiplex protein assays of 20 chemokines and growth factors and eight matrix metalloproteinases (MMPs)...
May 25, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28538585/reply-mechanical-micronization-of-lipoaspirates-squeeze-and-emulsification-techniques
#14
LETTER
Takanobu Mashiko, Szu-Hsien Wu, Jingwei Feng, Koji Kanayama, Kotaro Yoshimura
No abstract text is available yet for this article.
June 2017: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/28525415/effects-of-collagenase-digestion-and-stromal-vascular-fraction-supplementation-on-volume-retention-of-fat-grafts
#15
Jonathan B Olenczak, Scott A Seaman, Kant Y Lin, Angela Pineros-Fernandez, Catherine E Davis, Lisa S Salopek, Shayn M Peirce, Patrick S Cottler
OBJECTIVE: The use of autologous fat as a soft tissue filler has increased over the past decade in both reconstructive and aesthetic surgeries. Enhancement of autologous fat grafts with the addition of the stromal vascular fraction (SVF) has been reported to improve long-term volume retention. Stromal vascular fraction is most commonly isolated using enzymatic digestion, but it is unknown what effect the digestion process has on the adipocytes and SVF cells that comprise the graft. Some clinicians have reported use of enzymatically digested fat grafts to alter the physical properties of the tissue in specialized applications...
June 2017: Annals of Plastic Surgery
https://www.readbyqxmd.com/read/28486770/melatonin-promotes-survival-of-non-vascularized-fat-grafts-and-enhances-the-viability-and-migration-of-human-adipose-derived-stem-cells-via-down-regulation-of-acute-inflammatory-cytokines
#16
Shaun S Tan, Weiqing Zhan, Christopher J Phoon, Xiaolian Han, Diego Marre, Sholeh Boodhun, Jason A Palmer, Geraldine M Mitchell, Wayne A Morrison
Non-vascularized fat grafting is a valuable technique for soft tissue reconstruction but poor survival of fat in the host environment remains a problem. A process known as cell-assisted transfer is used to enhance fat graft retention by adding stromal vascular fraction, an adipose-derived stem cell (ASC) rich content to lipoaspirate. We have recently shown that the use of melatonin, a ROS scavenger, protects human ASCs from hydrogen peroxide induced oxidative stress and cell death in vitro but its role as a pharmacologic adjunct in clinical fat grafting has not been studied...
May 9, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28471290/is-the-transposition-of-the-nipple-areolar-complex-necessary-in-simon-grade-2b-gynecomastia-operations-using-suction-assisted-liposuction
#17
Burhan Özalp, Ömer Berköz, Mustafa Aydınol
OBJECTIVE: The aim of this study was to assess the efficacy of suction-assisted liposuction (SAL) in Simon grade 2b gynecomastia and its effect on sternal notch to nipple areola (SNN) distance. METHODS: A retrospective analysis was performed on 21 patients with grade 2b gynecomastia who underwent SAL. Preoperative and postoperative SNN distances of the patients were measured, the results were analysed using a Mann-Whitney U test and a p-value <.05 was accepted as statistically significant...
May 4, 2017: Journal of Plastic Surgery and Hand Surgery
https://www.readbyqxmd.com/read/28464475/isolation-of-cd248-expressing-stromal-vascular-fraction-for-targeted-improvement-of-wound-healing
#18
Elizabeth Brett, Elizabeth R Zielins, Monica Chin, Michael Januszyk, Charles P Blackshear, Michael Findlay, Arash Momeni, Geoffrey C Gurtner, Michael T Longaker, Derrick C Wan
Wound healing remains a global issue of disability, cost, and health. Addition of cells from the stromal vascular fraction (SVF) of adipose tissue has been shown to increase the rate of full thickness wound closure. This study aimed to investigate the angiogenic mechanisms of CD248+ SVF cells in the context of full thickness excisional wounds. Single cell transcriptional analysis was used to identify and cluster angiogenic gene-expressing cells, which was then correlated with surface marker expression. SVF cells isolated from human lipoaspirate were FACS sorted based on the presence of CD248...
May 2, 2017: Wound Repair and Regeneration
https://www.readbyqxmd.com/read/28452591/donor-dependent-variances-of-human-adipose-derived-stem-cells-in-respect-to-the-in-vitro-endothelial-cell-differentiation-capability
#19
Jascha Ell, Sybille Regn, Anna-Maria Buchberger, Achim von Bomhard, Thomas Stark, Jan-Thorsten Schantz, Katharina Storck
Human adipose-derived stem cells (ASC) have been shown to differentiate into mature adipocytes and to play an important role in creating the vasculature, necessary for white adipose tissue to function. To study the stimulatory capacity of ASC on endothelial progenitor cells we used a commercially available co-culture system (V2a - assay). ASC, isolated from lipoaspirates of 18 healthy patients, were co-cultured for 13 d on endothelial progenitor cells. Using anti CD31 immunostaining, cells that had undergone endothelial differentiation were quantified after the defined co-cultivation period...
January 2, 2017: Adipocyte
https://www.readbyqxmd.com/read/28445363/indomethacin-enhances-fat-graft-retention-by-up-regulating-adipogenic-genes-and-reducing-inflammation
#20
Weiqing Zhan, Shaun S Tan, Xiaolian Han, Jason A Palmer, Geraldine M Mitchell, Wayne A Morrison
BACKGROUND: Cell-assisted lipotransfer has been promisingly applied to restore soft-tissue defects in plastic surgery; however, the harvesting of stromal vascular fraction increases morbidity and poses potential safety hazards. The authors investigated whether adding indomethacin, an antiinflammatory proadipogenic drug, to the fat graft at the time of transplantation would enhance the final graft volume compared with cell-assisted lipotransfer. METHODS: In vitro, human adipose-derived stem cells were cultured in conditioned growth media supplemented with various doses of indomethacin to investigate adipogenesis and the expression of the adipogenic genes...
May 2017: Plastic and Reconstructive Surgery
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