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Manipulative medicine

Tariq Khan, Bilal Haider Abbasi, Mubarak Ali Khan
Manipulation in the light regimes combined with the effects of plant growth regulators (PGRs) and elicitors through plant cell culture technology is a promising strategy for enhancing the yield of medicinally important secondary metabolites. In this study, the effects of interplay between PGRs, elicitors and light regimes on cell cultures of F. indica have been investigated. The results showed that callus cultures resulted in maximum biomass formation (13.2 g/L) when incubated on solid MS (Murashige and Skoog) medium containing 1...
June 9, 2018: Journal of Photochemistry and Photobiology. B, Biology
Patrick M Shih
Humans have domesticated many plant species as indispensable sources of food, materials, and medicines. The dawning era of synthetic biology represents a means to further refine, redesign, and engineer crops to meet various societal and industrial needs. Current and future endeavors will utilize plants as the foundation of a bio-based economy through the photosynthetic production of carbohydrate feedstocks for the microbial fermentation of biofuels and bioproducts, with the end goal of decreasing our dependence on petrochemicals...
August 2018: Plant Science: An International Journal of Experimental Plant Biology
Yishen Tian, Chen Xin, Shuang Liu, Ying Liu, Shufeng Liu
Herein, a general protein conversion and analysis strategy was well developed for homogenous, label-free, and sensitive protein detection, which was on the basis of the affinity binding-induced Hg2+ releasing for protein conversion, and the succeeding Hg2+ doping-induced ZnSe quantum dot (QD) photoluminescence for signal readout. Two DNA motifs were designed, and each of which was conjugated with a protein-specific recognition ligand. The mercury ions were initially introduced into one DNA motif by T-Hg2+-T interaction...
June 15, 2018: ACS Sensors
Maxime M Mahe
The utilization of human pluripotent stem cells holds great promise in elucidating principles of developmental biology and applications in personalized and regenerative medicine. Breakthroughs from the last decade have allowed the scientific community to better understand and successfully manipulate human pluripotent stem cells using distinct differentiation strategies into a variety of target tissues. This manipulation relies solely on our understanding of developmental processes occurring in model organisms...
August 15, 2018: Brain Research
Y Moritani, M Usui, K Sano, K Nakazawa, T Hanatani, M Nakatomi, T Iwata, T Sato, W Ariyoshi, T Nishihara, K Nakashima
OBJECTIVE AND BACKGROUND: Human periodontal ligament mesenchymal stem cells (hPDLMSCs) are reported to be responsible for homeostasis and regeneration of periodontal tissue. Although hPDLMSCs are commonly cultured in monolayers, monolayer cultures have been reported as inferior to 3-dimensional cultures such as spheroids, which are spherical clusters of cells formed by self-assembly. The aim of this study was to examine the osteogenic phenotype of spheroids of hPDLMSCs, compared with monolayer cultures of hPDLMSC, in vitro and in vivo...
June 14, 2018: Journal of Periodontal Research
Xiaojing Wang, Guowei Wang, Sarah Zingales, Baodong Zhao
Repairing bone defects poses a major orthopedic challenge because current treatments are constrained by the limited regenerative capacity of human bone tissue. Novel therapeutic strategies, such as stem cell therapy and tissue engineering, have the potential to enhance bone healing and regeneration, and hence may improve quality of life for millions of people. However, the ex vivo expansion of stem cells and their in vivo delivery pose technical difficulties that hamper clinical translation and commercial development...
June 13, 2018: Tissue Engineering. Part B, Reviews
Sujay V Kharade, Haruto Kurata, Aaron Bender, Anna L Blobaum, Eric E Figueroa, Amanda M Duran, Meghan Kramer, Emily Days, Paige Vinson, Daniel Flores, Lisa M Satlin, Jens Meiler, C David Weaver, Craig W Lindsley, Corey R Hopkins, Jerod S Denton
The inward rectifier potassium (Kir) channel Kir4.1 (KCNJ10) carries out important physiological roles in epithelial cells of the kidney, astrocytes in the central nervous system, and stria vascularis of the inner ear. Loss-of-function mutations in KCNJ10 lead to EAST/SeSAME syndrome, which is characterized by epilepsy, ataxia, renal salt wasting, and sensorineural deafness. While genetic approaches have been indispensable for establishing the importance of Kir4.1 in the normal function of these tissues, the availability of pharmacological tools for acutely manipulating the activity of Kir4...
June 12, 2018: Molecular Pharmacology
Yinglu Feng, Zifei Yin, Daniel Zhang, Arun Srivastava, Chen Ling
The success of gene and cell therapy in clinic during the past two decades as well as our expanding ability to manipulate these biomaterials are leading to new therapeutic options for a wide range of inherited and acquired diseases. Combining conventional therapies with this emerging field is a promising strategy to treat those previously-thought untreatable diseases. Traditional Chinese medicine (TCM) has evolved for thousands of years in China and still plays an important role in human health. As part of the active ingredients of TCM, proteins and peptides have attracted long-term enthusiasm of researchers...
June 11, 2018: Current Protein & Peptide Science
Chen-Yi Li, Si-Yi Yu, Bao-Jun Guo, Wen-Yao Li, Jiao Yang, You-Ping Hu
OBJECTIVE: To analyze studies published in journals included in PubMed in terms of randomized controlled trials (RCTs) examining the therapeutic effect of acupuncture for simple obesity, so as to provide a reference for subsequent RCT-related designs and results. METHODS: RCT literature about acupuncture treatment of simple obesity from the PubMed database and from 2000 to 2016 was retrieved by using keywords of "obesity" "fat" "acupuncture" "joint application of acupuncture and herbal medicine" or "auricular point" "electroacupuncture", "acupoint application", or "cupping", followed by screening, extraction and evaluation of the design methodology and outcomes using Consolidated Standards for Reporting of Trials (CONSORT) and Standards for Reporting Interventions in Controlled Trials of Acupuncture (STRICTA)...
April 25, 2018: Zhen Ci Yan Jiu, Acupuncture Research
Reinout W Wiers, Marilisa Boffo, Matt Field
OBJECTIVE: Recently, the National Institutes of Health (NIH) redefined clinical trials to include any study involving behavioral or biomedical interventions. In line with a general framework from experimental medicine, we argue that it is crucial to distinguish between experimental laboratory studies aimed at revealing psychological mechanisms underlying behavior and randomized controlled trials (RCTs) in clinical samples aimed at testing the efficacy of an intervention. METHOD: As an illustration, we reviewed the current state of the evidence on the efficacy of cognitive bias modification (CBM) interventions in alcohol use disorders...
May 2018: Journal of Studies on Alcohol and Drugs
Christina Tsigalou, Elisavet Stavropoulou, Eugenia Bezirtzoglou
Sjogren's syndrome (SS) is an autoimmune disease, among the most common ones, that targets mainly the exocrine glands as well as extra-glandular epithelial tissues. Their lymphocytic infiltration leads to manifestations from other organs (e.g., kidneys, lungs, liver, or thyroid), apart from sicca symptoms (xerostomia and keratoconjunctivitis). SS is more prevalent in women than in men (9:1). Moreover, p.SS patients are in increased risk to develop lymphoma. Certain autoantibodies (e.g., antibodies against ribonucleoprotein autoantigens Ro-SSA and La-SSB) are ultimate hallmarks for the disease...
2018: Frontiers in Immunology
Ge Liu, Kai Wang, Shan Kuang, Ruobing Cao, Li Bao, Rui Liu, Hongwei Liu, Chaomin Sun
Cancer cells rewire their metabolism to satisfy the demands of uncontrolled proliferation and survival. The reprogramming of lipid metabolism supports tumor growth, metastasis, and therapy-resistance. Therefore, targeting lipid metabolic reprogramming is a potential cancer treatment strategy. We recently isolated the novel natural triterpene GL22 from Ganoderma leucocontextum, a traditional Chinese medicine. Here, we show that GL22 significantly inhibits the growth of the liver cancer cell line Huh7.5 in vitro and of Huh7...
June 7, 2018: Cell Death & Disease
Gail Van Kanegan, Julie Worley
Complementary alternative and integrative medicine (CAIM) modalities are increasingly sought after by patients with chronic health conditions such as substance use disorders (SUDs). Evidence suggests that CAIM interventions can improve symptoms commonly seen in patients with SUDs, such as stress, anxiety, depression, and pain. Mindfulness-based intervention (MBI), such as meditation, has proven effective for a variety of conditions, including SUDs. Other CAIM strategies, such as manipulative body practices and healing energy medicine techniques, are also showing some evidence of effectiveness...
May 1, 2018: Journal of Psychosocial Nursing and Mental Health Services
Shana L Boyle, David M Janicke, Michael E Robinson, Laura D Wandner
The purpose of the study was to investigate the influence of weight bias and demographic characteristics on the assessment of pediatric chronic pain. Weight status, race, and sex were manipulated in a series of virtual human (VH) digital images of children. Using a web-based platform, 96 undergraduate students with health care-related majors (e.g., Health Science, Nursing, Biology, and Pre-Medicine) read a clinical vignette and provided five ratings targeting the assessment of each VH child's pain. Students also answered a weight bias questionnaire...
June 4, 2018: Journal of Clinical Psychology in Medical Settings
Jian Kong, Zengjian Wang, Jaclyn Leiser, Domenic Minicucci, Robert Edwards, Irving Kirsch, Ajay D Wasan, Courtney Lang, Jessica Gerber, Siyi Yu, Vitaly Napadow, Ted J Kaptchuk, Randy L Gollub
Objectives: Expectation can significantly modulate pain and treatment effects. This study aims to investigate if boosting patients' expectancy can enhance the treatment of knee osteoarthritis (KOA), and its underlying brain mechanism. Methods: Seventy-four KOA patients were recruited and randomized to three groups: boosted acupuncture (with a manipulation to enhance expectation), standard acupuncture, or treatment as usual (TAU). Each patient underwent six treatments before being debriefed, and four additional treatments after being debriefed...
2018: NeuroImage: Clinical
Liad Holtzman, Charles A Gersbach
The eukaryotic epigenome has an instrumental role in determining and maintaining cell identity and function. Epigenetic components such as DNA methylation, histone tail modifications, chromatin accessibility, and DNA architecture are tightly correlated with central cellular processes, while their dysregulation manifests in aberrant gene expression and disease. The ability to specifically edit the epigenome holds the promise of enhancing understanding of how epigenetic modifications function and enabling manipulation of cell phenotype for research or therapeutic purposes...
May 31, 2018: Annual Review of Genomics and Human Genetics
Melissa Fernandes, Ana Eufrásio, João Bonifácio, João Marcelino
Airway management is one of the most important anaesthetist's skills as major complications of airway management, although rare, can be among the most life threatening in medicine. Crouzon syndrome is a rare condition with physical characteristics that can result in difficult airway manipulation. A correct preanaesthetic evaluation and a planned preinduction strategy should be designed to facilitate intubation.
May 30, 2018: BMJ Case Reports
Leemon B McHenry
OBJECTIVE: Examination of de-classified Monsanto documents from litigation in order to expose the impact of the company's efforts to influence the reporting of scientific studies related to the safety of the herbicide, glyphosate. METHODS: A set of 141 recently de-classified documents, made public during the course of pending toxic tort litigation, In Re Roundup Products Liability Litigation were examined. RESULTS: The documents reveal Monsanto-sponsored ghostwriting of articles published in toxicology journals and the lay media, interference in the peer review process, behind-the-scenes influence on retraction and the creation of a so-called academic website as a front for the defense of Monsanto products...
May 21, 2018: International Journal of Risk & Safety in Medicine
Yinshen Wee, Amanda N Moore, Shihai Jia, Jing Zhou, John S Colombo, Rena N D'Souza
Hydrogels are homogenous materials that are limited in their ability to form oriented multilayered architecture in three-dimensional (3D) tissue constructs. Current techniques have led to advancements in this area. Such techniques often require extra devices and/or involve complex processes that are inaccessible to many laboratories. Here is described a one-step methodology that permits reliable alignment of cells into multiple layers using a self-assembling multidomain peptide (MDP) hydrogels. We characterized the structural features, viability, and molecular properties of dental pulp cells fabricated with MDP and demonstrated that manipulation of the layering of cells in the scaffolds was achieved by decreasing the weight by volume percentage (w/v%) of MDP contained within the scaffold...
May 1, 2018: SLAS Technology
Chris Warren, Jordan Keys, Stacey Pierce-Talsma
No abstract text is available yet for this article.
June 1, 2018: Journal of the American Osteopathic Association
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