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Manipulative medicine

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https://www.readbyqxmd.com/read/28229589/production-of-cinnamyl-alcohol-glucoside-from-glucose-in-escherichia-coli
#1
Wei Zhou, Huiping Bi, Yibin Zhuang, Qinglin He, Hua Yin, Tao Liu, Yanhe Ma
Rosin, a cinnamyl alcohol glucoside, is one of the important ingredients in Rhodiola rosea, which is a valuable medicinal herb used for centuries. Rosin displayed multiple biological activities. The traditional method for producing rosin and derivatives is direct extraction from R. rosea, which suffers from limited availability of natural resources and complicated purification procedure. In this work, we achieved de novo biosynthesis of rosin in Escherichia coli. Firstly, a biosynthetic pathway of aglycon cinnamyl alcohol from phenylalanine was constructed...
February 23, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28225201/edible-insects-in-china-utilization-and-prospects
#2
REVIEW
Ying Feng, Xiao-Ming Chen, Min Zhao, Zhao He, Long Sun, Cheng-Ye Wang, Wei-Feng Ding
The use of edible insects has a long history in China, where they have been consumed for more than two thousand years. In general, the level of acceptance is high for the consumption of insects in China. Many studies on edible insects have been conducted in the last twenty years, and the scope of the research includes the culture of entomophagy and the identification, nutritional value, farming and breeding of edible insects, in addition to food production and safety. Currently, 324 species of insects from 11 orders are documented that are either edible or associated with entomophagy in China, which include the common edible species, some less commonly consumed species, and some medicinal insects...
February 22, 2017: Insect Science
https://www.readbyqxmd.com/read/28216951/a-qualitative-insight-on-complementary-and-alternative-medicines-used-by-hypertensive-patients
#3
Inas Rifaat Ibrahim, Mohamed Azmi Hassali, Fahad Saleem, Haydar F Al Tukmagi
BACKGROUND: The self-treatment with complementary and alternative medicines (CAMs) in chronic diseases is portraying an expanding trend worldwide. Yet, little is known concerning patients' motives to use CAM in the control of blood pressure. OBJECTIVE: This study aims to explore the self-use of CAM in the management of hypertension and explore patients' attitudes, perceived benefits, and disclosure to the physician. MATERIALS AND METHODS: A qualitative technique was adopted and face-to-face interviews, using a validated interview guide, were carried out among twenty hypertensive patients...
October 2016: Journal of Pharmacy & Bioallied Sciences
https://www.readbyqxmd.com/read/28213959/combining-induced-pluripotent-stem-cells-and-genome-editing-technologies-for-clinical-applications
#4
Chia-Yu Chang, Hsiao-Chien Ting, Hong-Lin Su, Jing-Ren Jeng
In this review, we introduce current developments in induced pluripotent stem cells (iPSCs), site-specific nuclease (SSN)-mediated genome editing tools, and the combined application of these two novel technologies in biomedical research and therapeutic trials. The sustainable pluripotent property of iPSCs in vitro not only provides unlimited cell sources for basic research but also benefits precision medicines for human diseases. In addition, rapidly evolving SSN tools efficiently tailor genetic manipulations for exploring gene functions and can be utilized to correct genetic defects of congenital diseases in the near future...
February 17, 2017: Cell Transplantation
https://www.readbyqxmd.com/read/28213697/danger-of-injudicious-use-of-tui-na-therapy-in-ankylosing-spondylitis
#5
Gen Zou, Guyi Wang, Jinxiu Li, Guobao Wu, Jia Huang, Siyang Huang
Tui-na is a very important component of Chinese medicine. It is a well-respected treatment modality known to be helpful and safe for a wide range of conditions. It can be considered as a certain kind of massage which is performed at acupoints, meridians, and collaterals by pushing, finger twisting, grasping thumb waving pressing, patting, palm twisting, and other manipulation techniques. At present, it is extensively used for relieving pain and stiffness associated with ankylosing spondylitis in China, even though there is a lack of evidence to support its validation and feasibility...
February 17, 2017: European Spine Journal
https://www.readbyqxmd.com/read/28212287/big-data-analytics-for-genomic-medicine
#6
REVIEW
Karen Y He, Dongliang Ge, Max M He
Genomic medicine attempts to build individualized strategies for diagnostic or therapeutic decision-making by utilizing patients' genomic information. Big Data analytics uncovers hidden patterns, unknown correlations, and other insights through examining large-scale various data sets. While integration and manipulation of diverse genomic data and comprehensive electronic health records (EHRs) on a Big Data infrastructure exhibit challenges, they also provide a feasible opportunity to develop an efficient and effective approach to identify clinically actionable genetic variants for individualized diagnosis and therapy...
February 15, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28203065/oral-drug-therapy-in-elderly-with-dysphagia-between-a-rock-and-a-hard-place
#7
REVIEW
Serena Logrippo, Giovanna Ricci, Matteo Sestili, Marco Cespi, Letizia Ferrara, Giovanni F Palmieri, Roberta Ganzetti, Giulia Bonacucina, Paolo Blasi
Demographic indicators forecast that by 2050, the elderly will account for about one-third of the global population. Geriatric patients require a large number of medicines, and in most cases, these products are administered as solid oral solid dosage forms, as they are by far the most common formulations on the market. However, this population tends to suffer difficulties with swallowing. Caregivers in hospital geriatric units routinely compound in solid oral dosage forms for dysphagic patients by crushing the tablets or opening the capsules to facilitate administration...
2017: Clinical Interventions in Aging
https://www.readbyqxmd.com/read/28191781/direct-isolation-and-characterization-of-human-nephron-progenitors
#8
Stefano Da Sacco, Matthew E Thornton, Astgik Petrosyan, Maria Lavarreda-Pearce, Sargis Sedrakyan, Brendan H Grubbs, Roger E De Filippo, Laura Perin
Mature nephrons originate from a small population of uninduced nephrogenic progenitor cells (NPs) within the cap mesenchyme. These cells are characterized by the coexpression of SIX2 and CITED1. Many studies on mouse models as well as on human pluripotent stem cells have advanced our knowledge of NPs, but very little is known about this population in humans, since it is exhausted before birth and strategies for its direct isolation are still limited. Here we report an efficient protocol for direct isolation of human NPs without genetic manipulation or stepwise induction procedures...
February 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28191769/suppression-of-transforming-growth-factor-%C3%AE-signaling-delays-cellular-senescence-and-preserves-the-function-of-endothelial-cells-derived-from-human-pluripotent-stem-cells
#9
Hao Bai, Yongxing Gao, Dixie L Hoyle, Tao Cheng, Zack Z Wang
Transplantation of vascular cells derived from human pluripotent stem cells (hPSCs) offers an attractive noninvasive method for repairing the ischemic tissues and for preventing the progression of vascular diseases. Here, we found that in a serum-free condition, the proliferation rate of hPSC-derived endothelial cells is quickly decreased, accompanied with an increased cellular senescence, resulting in impaired gene expression of endothelial nitric oxide synthase (eNOS) and impaired vessel forming capability in vitro and in vivo...
February 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28191764/neural-progenitor-like-cells-induced-from-human-gingiva-derived-mesenchymal-stem-cells-regulate-myelination-of-schwann-cells-in-rat-sciatic-nerve-regeneration
#10
Qunzhou Zhang, Phuong Nguyen, Qilin Xu, Wonse Park, Sumin Lee, Akihiro Furuhashi, Anh D Le
Regeneration of peripheral nerve injury remains a major clinical challenge. Recently, mesenchymal stem cells (MSCs) have been considered as potential candidates for peripheral nerve regeneration; however, the underlying mechanisms remain elusive. Here, we show that human gingiva-derived MSCs (GMSCs) could be directly induced into multipotent NPCs (iNPCs) under minimally manipulated conditions without the introduction of exogenous genes. Using a crush-injury model of rat sciatic nerve, we demonstrate that GMSCs transplanted to the injury site could differentiate into neuronal cells, whereas iNPCs could differentiate into both neuronal and Schwann cells...
February 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28174251/a-process-engineering-approach-to-increase-organoid-yield
#11
Natasha Arora, Jasmin Imran Alsous, Jacob W Guggenheim, Michael Mak, Jorge Munera, James M Wells, Roger D Kamm, H Harry Asada, Stanislav Y Shvartsman, Linda G Griffith
Temporal manipulation of the in vitro environment and growth factors can direct differentiation of human pluripotent stem cells into organoids, aggregates with multiple tissue-specific cell types and three-dimensional structure mimicking native organs. A mechanistic understanding of early organoid formation is essential for improving the robustness of these methods, which is necessary prior to use in drug development and regenerative medicine. We investigated intestinal organoid emergence, focusing on measurable parameters of hindgut spheroids, the intermediate step between definitive endoderm and mature organoids...
February 7, 2017: Development
https://www.readbyqxmd.com/read/28170177/pluripotent-nontumorigenic-adipose-tissue-derived-muse-cells-have-immunomodulatory-capacity-mediated-by-transforming-growth-factor-%C3%AE-1
#12
María L Gimeno, Florencia Fuertes, Andres E Barcala Tabarrozzi, Alejandra I Attorressi, Rodolfo Cucchiani, Luis Corrales, Talita C Oliveira, Mari C Sogayar, Leticia Labriola, Ricardo A Dewey, Marcelo J Perone
Adult mesenchymal stromal cell-based interventions have shown promising results in a broad range of diseases. However, their use has faced limited effectiveness owing to the low survival rates and susceptibility to environmental stress on transplantation. We describe the cellular and molecular characteristics of multilineage-differentiating stress-enduring (Muse) cells derived from adipose tissue (AT), a subpopulation of pluripotent stem cells isolated from human lipoaspirates. Muse-AT cells were efficiently obtained using a simple, fast, and affordable procedure, avoiding cell sorting and genetic manipulation methods...
January 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28160763/the-use-of-complementary-and-alternative-medicine-by-patients-undergoing-hemodialysis
#13
Özlem Ceyhan, Songul Göris, Nevin Dogan, Sevda Korkut Bayındır
Context • Hemodialysis (HD) is a frequently used method for the treatment of chronic renal failure. Patients show many symptoms and problems, depending on the success of HD and the disease. Today, one of the most commonly used methods, in addition to pharmacological treatments, for ensuring symptom control and reinforcing a patient's well-being is complementary and alternative medicine (CAM). Objectives • The study was conducted to determine the benefits of the use of CAM for patients undergoing HD. Design • The study was descriptive...
January 2017: Alternative Therapies in Health and Medicine
https://www.readbyqxmd.com/read/28159719/improvement-of-osteogenesis-in-dental-pulp-pluripotent-like-stem-cells-by-oligopeptide-modified-poly-%C3%A3-amino-ester-s
#14
Raquel Núñez-Toldrà, Pere Dosta, Sheyla Montori, Víctor Ramos, Maher Atari, Salvador Borrós
: Controlling pluripotent stem cell differentiation via genetic manipulation is a promising technique in regenerative medicine. However, the lack of safe and efficient delivery vehicles limits this application. Recently, a new family of poly (β-amino ester)s (pBAEs) with oligopeptide-modified termini showing high transfection efficiency of both siRNA and DNA plasmid has been developed. In this study, oligopeptide-modified pBAEs were used to simultaneously deliver anti-OCT3/4 siRNA, anti-NANOG siRNA, and RUNX2 plasmid to cells from the dental pulp with pluripotent-like characteristics (DPPSC) in order to promote their osteogenic differentiation...
January 31, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28150746/rapid-cell-separation-with-minimal-manipulation-for-autologous-cell-therapies
#15
Alban J Smith, Richard D O'Rorke, Akshay Kale, Roberts Rimsa, Matthew J Tomlinson, Jennifer Kirkham, A Giles Davies, Christoph Wälti, Christopher D Wood
The ability to isolate specific, viable cell populations from mixed ensembles with minimal manipulation and within intra-operative time would provide significant advantages for autologous, cell-based therapies in regenerative medicine. Current cell-enrichment technologies are either slow, lack specificity and/or require labelling. Thus a rapid, label-free separation technology that does not affect cell functionality, viability or phenotype is highly desirable. Here, we demonstrate separation of viable from non-viable human stromal cells using remote dielectrophoresis, in which an electric field is coupled into a microfluidic channel using shear-horizontal surface acoustic waves, producing an array of virtual electrodes within the channel...
February 2, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28150318/determine-exogenous-human-ddah2-gene-function-in-rabbit-bone-marrow-derived-endothelial-progenitor-cells-in-vitro
#16
Sara Shoeibi, Shabnam Mohammadi, Hamid Reza Sadeghnia, Elahe Mahdipour, Majid Ghayour-Mobarhan
The in vitro amplification of endothelial progenitor cells (EPCs) is an important method because of its role in gene transferring and regenerative medicine. In this study, we isolated rabbit bone marrow-derived EPCs to further manipulation and overexpression of dimethylarginine dimethylaminohydrolase (DDAH) in EPCs. Isolated EPCs were cultured, expanded in endothelial basal medium. Morphology of EPCs and expression levels of surface markers detected using immunocytochemistry staining and through the use of flow cytometery...
February 1, 2017: Cell Biochemistry and Function
https://www.readbyqxmd.com/read/28134023/effects-of-ionizing-radiation-on-dna-methylation-from-experimental-biology-to-clinical-applications
#17
Isabelle R Miousse, Kristy R Kutanzi, Igor Koturbash
PURPOSE: Ionizing radiation (IR) is a ubiquitous environmental stressor with genotoxic and epigenotoxic capabilities. Terrestrial IR, predominantly a low-linear energy transfer (LET) radiation, is being widely utilized in medicine, as well as in multiple industrial applications. Additionally, an interest in understanding the effects of high-LET irradiation is emerging due to the potential of exposure during space missions and the growing utilization of LET radiation in medicine. CONCLUSIONS: In this review, we summarize the current knowledge of the effects of IR on DNA methylation, a key epigenetic mechanism regulating the expression of genetic information...
January 30, 2017: International Journal of Radiation Biology
https://www.readbyqxmd.com/read/28134003/development-of-a-patient-specific-3d-printed-liver-model-for-preoperative-planning
#18
Marta J Madurska, Matthieu Poyade, David Eason, Paul Rea, Angus J M Watson
INTRODUCTION: Liver surgery is widely used as a treatment modality for various liver pathologies. Despite significant improvement in clinical care, operative strategies, and technology over the past few decades, liver surgery is still risky, and optimal preoperative planning and anatomical assessment are necessary to minimize risks of serious complications. 3D printing technology is rapidly expanding, and whilst appliactions in medicine are growing, but its applications in liver surgery are still limited...
January 1, 2017: Surgical Innovation
https://www.readbyqxmd.com/read/28130394/application-of-microfluidic-technologies-to-human-assisted-reproduction
#19
REVIEW
Gary D Smith, Shuichi Takayama
Microfluidics can be considered both a science and a technology. It is defined as the study of fluid behavior at a sub-microliter level and the investigation into its application to cell biology, chemistry, genetics, molecular biology and medicine. There are at least two characteristics of microfluidics, mechanical and biochemical, which can be influential in the field of mammalian gamete and preimplantation embryo biology. These microfluidic characteristics can assist in basic biological studies on sperm, oocyte and preimplantation embryo structure, function and environment...
January 27, 2017: Molecular Human Reproduction
https://www.readbyqxmd.com/read/28128301/discovering-novel-phenotypes-with-automatically-inferred-dynamic-models-a-partial-melanocyte-conversion-in-xenopus
#20
Daniel Lobo, Maria Lobikin, Michael Levin
Progress in regenerative medicine requires reverse-engineering cellular control networks to infer perturbations with desired systems-level outcomes. Such dynamic models allow phenotypic predictions for novel perturbations to be rapidly assessed in silico. Here, we analyzed a Xenopus model of conversion of melanocytes to a metastatic-like phenotype only previously observed in an all-or-none manner. Prior in vivo genetic and pharmacological experiments showed that individual animals either fully convert or remain normal, at some characteristic frequency after a given perturbation...
January 27, 2017: Scientific Reports
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