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https://www.readbyqxmd.com/read/29793199/effects-of-acute-and-chronic-exposures-of-fluoxetine-on-the-chinese-fish-topmouth-gudgeon-pseudorasbora-parva
#1
Hongxing Chen, Xiangfeng Zeng, Lei Mu, Liping Hou, Bin Yang, Jianliang Zhao, Daniel Schlenk, Wu Dong, Lingtian Xie, Qianru Zhang
Fluoxetine is a selective serotonin reuptake inhibitor used as an antidepressant and has been frequently detected in aquatic environments. However, its effects in fish from Asia remain relatively less studied. In this study, the topmouth gudgeon Pseudorasbora parva was exposed to 0, 50, and 200 µg/L of fluoxetine for 4 h and 42 d. The effects of fluoxetine on biometrics were compared to biochemical endpoints indicative of stress in different fish tissues (brain, liver, gills and intestine) following exposures...
May 21, 2018: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/29793180/insulin-is-a-potential-antioxidant-for-diabetes-associated-cognitive-decline-via-regulating-nrf2-dependent-antioxidant-enzymes
#2
Ying Song, Wei Ding, Yun Bei, Yan Xiao, Hai-Da Tong, Li-Bo Wang, Li-Yao Ai
PURPOSE: To investigate the neuroprotective effects of insulin on diabetic encephalopathy and its mechanism. EXPERIMENTAL AND APPROACH: The diabetic model was established by injection of streptozotocin. Behavior examinations were conducted by the Morris water maze. Histopathological alterations were detected by HE staining. ROS, CAT levels and SOD activity were measured using a microplate reader. In vitro, the viability of wild type and knock-down PC12 cells was detected by MTT assay, the morphology of cells was monitored under a microscope...
May 21, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29793145/mechanistic-approach-to-study-conjugation-of-nanoparticles-for-biomedical-applications
#3
Imran Uddin
Interaction of nanoparticles with biological systems turns out to be vibrant for their efficient application in biomedical field. Here, we have shown antibiotic amakicin loaded nanoparticles are responsible for the dual role as reducing and stabilizing the silver nanoparticles without the use of any undesired chemicals. Synthesized nanoparticles are well-dispersed having quasi spherical morphology with an average particle size around 10-11 nm. Crystallinity of nanoparticles was measured using selected area electron diffraction (SAED) and powder XRD analysis which show that particles are perfectly crystalline with cubic phase of geometry...
May 16, 2018: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/29793141/new-contributions-to-gruberia-lanceolata-gruber-1884-kahl-1932-based-on-analyses-of-multiple-populations-and-genes-ciliophora-heterotrichea-gruberiidae
#4
Xiangrui Chen, Shahed Uddin Ahmed Shazib, Ji Hye Kim, Min Seok Kim, Mann Kyoon Shin
Gruberia Kahl, 1932 is a species-poor genus comprising only seven named species. Most of these species have not been reinvestigated since the original reports. In the present work, we investigated the taxonomy and phylogeny of Gruberia lanceolata (Gruber, 1884) Kahl, 1932 based on analyses of morphology and multiple gene sequences from four South Korean populations. This species is mainly characterized by a well-developed peristome region, segmented paroral membrane, and moniliform macronucleus. Some morphological features were not stable among the four populations investigated, such as body shape and size, cell color, and the ratio of oral length to body length...
May 9, 2018: European Journal of Protistology
https://www.readbyqxmd.com/read/29793125/assessment-of-cam-morphology-of-the-hip-with-ultra-sound-imaging-by-physical-therapists-is-reliable-and-valid
#5
Liliane Mandema, Igor Tak, Brigitte Mooij, Ruud Groeneweg, Eric Bakker, Peter van Veldhoven
BACKGROUND: Femoroacetabular impingement syndrome is considered present when a combination of clinical symptoms, signs and morphological features of the hip co-exist. Imaging is increasingly popular in primary health care settings with physical therapists performing ultrasound (US). OBJECTIVE: The aim of this study was to assess the diagnostic accuracy and inter-rater reliability of ultrasound in determining presence of cam morphology. DESIGN: A concurrent validity and inter-rater reliability study...
April 14, 2018: Physical Therapy in Sport
https://www.readbyqxmd.com/read/29793112/3d-printed-membrane-as-an-alternative-to-amniotic-membrane-for-ocular-surface-conjunctival-defect-reconstruction-an-in-vitro-in-vivo-study
#6
Shima Dehghani, Morteza Rasoulianboroujeni, Hamed Ghasemi, Saeed Heidari Keshel, Zohreh Nozarian, Mohammad Naser Hashemian, Mehran Zarei-Ghanavati, Golshan Latifi, Reza Ghaffari, Zhanfeng Cui, Hua Ye, Lobat Tayebi
BACKGROUND: The aim of this study was to evaluate the surgical handling and clinical applicability of a specific 3D-printed membrane design fabricated using a gelatin, elastin and sodium hyaluronate blend for conjunctival reconstruction and compare it with amniotic membrane (AM), which is normally used in such surgeries. METHODS: 3D printing technique was employed to fabricate the membrane based on gradient design. Prior to printing, rheometry was employed to optimize the ink composition...
May 11, 2018: Biomaterials
https://www.readbyqxmd.com/read/29793083/optimization-adsorption-of-norfloxacin-onto-polydopamine-microspheres-from-aqueous-solution-kinetic-equilibrium-and-adsorption-mechanism-studies
#7
Yibei Wan, Xiao Liu, Penglei Liu, Lei Zhao, Weihua Zou
Polydopamine microspheres (PDMPs) synthesized by a facile solution oxidation method were adopted as a potential adsorbent for the removal of Norfloxacin (NOR) from aqueous solution. The morphologies and properties of PDMPs were characterized using TEM, SEM, FTIR and pHPZC . Parameters effects such as contact time, initial pH, initial concentration and ionic strength on the adsorption capacity of NOR onto PDMPs were studied. To maximize NOR removal from liquid phase, Box-Behnken experimental design (BBD) combined with response surface modeling (RSM) was employed based on the 17 preliminary experiments at 308 K...
May 21, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/29793060/a-computational-framework-for-the-detection-of-subcortical-brain-dysmaturation-in-neonatal-mri-using-3d-convolutional-neural-networks
#8
Rafael Ceschin, Alexandria Zahner, William Reynolds, Jenna Gaesser, Giulio Zuccoli, Cecilia W Lo, Vanathi Gopalakrishnan, Ashok Panigrahy
Deep neural networks are increasingly being used in both supervised learning for classification tasks and unsupervised learning to derive complex patterns from the input data. However, the successful implementation of deep neural networks using neuroimaging datasets requires adequate sample size for training and well-defined signal intensity based structural differentiation. There is a lack of effective automated diagnostic tools for the reliable detection of brain dysmaturation in the neonatal period, related to small sample size and complex undifferentiated brain structures, despite both translational research and clinical importance...
May 21, 2018: NeuroImage
https://www.readbyqxmd.com/read/29792966/one-step-fabrication-of-chitosan-fe-oh-3-beads-for-efficient-adsorption-of-anionic-dyes
#9
XinxinYang, Yumei Li, Hongmei Gao, Chuying Wang, Xiaohuan Zhang, Hongli Zhou
In this study, we reported the one-step synthesis of chitosan-Fe(OH)3 beads without the use of acid solvent, which could be used as effective adsorbents for anionic dyes removal. The preparation process was easy and green. The as-prepared beads were characterized for structural and morphological analysis in detail using several techniques, such as Scanning Electron Microscope, Fourier Transform Infrared Spectroscopy, X-Ray Diffraction, X-ray photoelectron spectroscopy and Thermogravimetric analysis. The content of Fe(OH)3 in chitosan-Fe(OH)3 beads was 54...
May 21, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29792961/composite-films-of-regenerate-cellulose-with-chitosan-and-polyvinyl-alcohol-evaluation-of-water-adsorption-mechanical-and-optical-properties
#10
Patricia Cazón, Manuel Vázquez, Gonzalo Velazquez
The aim of this study was to develop composite films from cellulose, chitosan and polyvinyl alcohol to obtain environmentally friendly materials. Toughness, burst strength, distance to burst and water adsorption properties were measured and analysed as a function of cellulose (3-5%), chitosan (0-1%) and polyvinyl alcohol (0-4%) contents. Polynomial models were obtained. Light-barrier properties, transparency, morphology, structural and thermal analyses were assessed. Results showed that chitosan and polyvinyl alcohol enhanced the mechanical properties of cellulose-based films...
May 21, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29792960/hybrid-chitosan-tio-2-zns-prepared-under-mild-conditions-with-visible-light-driven-photocatalytic-activity
#11
A Jbeli, A M Ferraria, A M Botelho do Rego, S Boufi, S Bouattour
Thin films of chitosan (CS) coated on glass substrates were used as support for the immobilization of nanostructures TiO2 /ZnS via a mild chemical process followed by a hydrothermal treatment at 90 °C. The preparation route involved the coating of the CS film on the glass substrate followed by the deposition of a thin layer of Ti(OBu)4 precursor and immersion in a solution of Zn(O2 CCH3 )2 and Na2 S. After a hydrothermal treatment at 90 °C, an even layer of TiO2 anatase decorated by ZnS nanorods was generated...
May 21, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29792959/fabrication-and-cytotoxicity-assessment-of-cellulose-nanofibrils-using-bassia-eriophora-biomass
#12
Jegan Athinarayanan, Vaiyapuri Subbarayan Periasamy, Ali A Alshatwi
Cellulose nanofibrils (CNs) are eco-friendly, biodegradable, biocompatible, renewable, cost-effective, and possess excellent mechanical properties. We fabricated CNs from Bassia eriophora biomass, and the structure and morphology were investigated by transmission electron microscopy that revealed 2-6 μm long fibrillated structures with diameters of 15-40 nm. CNs biocompatibility was assessed using in vitro based assays, including cell viability assay, AO/EB staining, Hoechst staining, JC-1 staining, and gene expression analysis...
May 21, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29792941/in-situ-analysis-of-grain-rotation-and-lattice-strain-within-a-magnesium-polycrystal-based-on-synchrotron-polychromatic-x-ray-diffraction-technique-i-prior-to-twin
#13
Li Li, Yuanzhi Wu, Jie Wu
Hexagonal-close-packed structure aggregates exhibit complicated deformation behaviors, involving different slips and twinning. Synchrotron polychromatic X-ray microdiffraction (micro-XRD) was utilized to study in situ an extruded Mg-3Al-1Zn strip subjected to uniaxial tension. The evolution of grain rotation and lattice strain was analyzed under the load levels from 12 to 73 MPa. The micro-XRD data was used to map an area of 396 × 200 μm within the region of interest. The experimental set-up and X-ray diffraction microscopy in two dimensions allow the morphology, orientation and strain of the target grain to be determined at the submicron size...
May 17, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/29792891/pcl-eug-scaffolds-with-tunable-stiffness-can-regulate-macrophage-secretion-behavior
#14
REVIEW
Y Z B Xue, Yiming Niu, B Tang, C M Wang
Osteoarthritis (OA) is a prevalent joint disorder worldwide. Recent studies suggested that macrophages play an important role in the progression of OA. However, the detailed pathology related to macrophages is still ambiguous, especially those related to mechanotransduction. In this study, PCL/EUG composite scaffolds were first fabricated by electrospinning. The stiffness of as-fabricated scaffolds was controlled by adjusting the PCL-versus-EUG ratio. The mechanical properties, structural characterics and chemical composition of the scaffolds were investigated using various materials characterization techniques...
May 21, 2018: Progress in Biophysics and Molecular Biology
https://www.readbyqxmd.com/read/29792868/ah6809-decreases-production-of-inflammatory-mediators-by-pge-2-ep2-camp-signaling-pathway-in-an-experimentally-induced-pure-cerebral-concussion-in-rats
#15
Ping Li, Hongyan Jiang, Haiying Wu, Deye Wu, Hengxi Li, Jianyun Yu, Jianghua Lai
Increasing evidence suggests that PGE2 metabolic pathway is involved in pathological changes of the secondary brain injury after traumatic brain injury. However, the underlying mechanisms, in particular, the correlation between various key enzymes and the brain injury, has remained to be fully explored. More specifically, it remains to be ascertained whether AH6809 (an EP2 receptor antagonist) would interfere with the downstream of the PGE2 , regulate the inflammatory mediators and improve neuronal damage in the hippocampus by PGE2 - EP2 - cAMP signaling pathway...
May 21, 2018: Brain Research
https://www.readbyqxmd.com/read/29792857/tgf-%C3%AE-plays-a-vital-role-in-triple-negative-breast-cancer-tnbc-drug-resistance-through-regulating-stemness-emt-and-apoptosis
#16
Xiaodan Xu, Lu Zhang, Xiaogang He, Ping Zhang, Caihong Sun, Xiaojun Xu, Yaojuan Lu, Feifei Li
Triple negative breast cancer (TNBC) is the most malignant subtype of breast cancer in which the cell surface lacks usual targets for drug to exhibit its effects. Epirubicin (Epi) is widely used for TNBC, but a substantial number of patients develop Epi resistance that is usually associated with poor prognosis. Transforming growth factor (TGF-β) is a multifunctional cytokine. In recent study, it appears that TGF-β influences the cancer stem cell population, thus, the drug resistance of cancer may also be affected...
May 21, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29792795/tuning-the-morphology-of-nanostructured-peptide-films-by-introduction-of-a-secondary-structure-conformational-constraint-a-case-study-of-hierarchical-self-assembly
#17
Marta De Zotti, Beatrice Muzzi, Emanuela Gatto, Benedetta Di Napoli, Claudia Mazzuca, Antonio Palleschi, Ernesto Placidi, Fernando Formaggio, Claudio Toniolo, Mariano Venanzi
Peptide self-assembly is ubiquitous in nature. It governs the organization of proteins, controlling their folding kinetics and preserving their structure stability and bioactivity. In this connection, model oligopeptides may give important insights on the molecular mechanisms and elementary forces driving the formation of supramolecular structures. In this contribution we show that a single residue substitution, i.e. Aib (α-aminoisobutyric acid) in place of Ala at position 4 of an -(L-Ala)5- homo-oligomer, strongly alters the aggregation process...
May 24, 2018: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/29792783/phylogenetic-studies-in-genabea-myrmecocystis-and-related-genera
#18
Pablo Alvarado, Rosanne Healy, Gabriel Moreno, Julio Cabero, Markus Scholler, Anja Schneider, Alfredo Vizzini, Vasileios Kaounas, Josep Maria Vidal, Gunnar Hensel, Enrique Rubio, Alija Mujic, Matthew E Smith
A multigene phylogenetic analysis of Genabea, Myrmecocystis, and related genera in Pyronemataceae was conducted to establish genetically supported generic limits. The nuc rDNA 28S gene, translation elongation factor 1-α gene (TEF1), and RNA polymerase II second-largest subunit gene (RPB2) significantly supported the monophyly of several distinct genera of hypogeous Pyronemataceae. The genetic identity of Genabea fragilis is established, and the new species Genabea hyalospora, G. urbana, Myrmecocystis mediterranea, and M...
March 26, 2018: Mycologia
https://www.readbyqxmd.com/read/29792777/dismantling-a-complex-of-anther-smuts-microbotryum-on-carnivorous-plants-in-the-genus-pinguicula
#19
Rebekka Ziegler, Matthias Lutz, Jolanta Piątek, Marcin Piątek
The anther smuts of the genus Microbotryum are known from host plant species belonging to the Caryophyllaceae, Dipsacaceae, Lamiaceae, Lentibulariaceae, Montiaceae, and Primulaceae. Of these, the anther smuts on Caryophyllaceae, in particular on Silene spp., are best known because they include model organisms studied in many disciplines of fungal biology. For Microbotryum species parasitic on Caryophyllaceae, a high degree of host specificity was revealed and several cryptic species were described. In contrast, the host specificity within Microbotryum pinguiculae occurring in anthers of different Pinguicula species (Lentibulariaceae) has not been investigated in detail until now...
March 15, 2018: Mycologia
https://www.readbyqxmd.com/read/29792748/portal-vein-thrombosis-imaging-the-spectrum-of-disease-with-an-emphasis-on-mri-features
#20
Reena C Jha, Satinderpal Singh Khera, Amit Dinesh Kalaria
OBJECTIVE: The purpose of this article is to review the classic and uncommon imaging findings of portal vein thrombosis (PVT) (acute, chronic, congenital, and septic thrombus) as visualized on multiple modalities, with an emphasis on MRI findings. Additional aims are to understand the imaging of obliterative portal venopathy and its clinical significance, appreciate morphologic changes of the biliary system that may accompany PVT, and recognize changes in liver enhancement patterns seen with PVT related to the hepatic arterial buffer response...
May 24, 2018: AJR. American Journal of Roentgenology
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