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https://www.readbyqxmd.com/read/28651420/-effects-of-hypoxia-inducible-factor-1%C3%AE-on-p311-and-its-influence-on-the-migration-of-murine-epidermal-stem-cells
#1
Z D Xu, H S Li, S Wang, W F He, J Wu, G X Luo
Objective: To explore the effects of hypoxia inducible factor-1α (HIF-1α) on P311 and its influence on the migration of murine epidermal stem cells (ESCs) under hypoxia in vitro. Methods: Two kinds of murine ESCs were isolated and obtained from 15 neonatal wild-type C57BL/6J mice and 5 congeneric source P311 gene knock-out mice, respectively. The first passage of cells were used in the following experiments after morphologic observation and detection of expression of cell surface markers CD71 and CD49f with flow cytometer...
May 20, 2017: Zhonghua Shao Shang za Zhi, Zhonghua Shaoshang Zazhi, Chinese Journal of Burns
https://www.readbyqxmd.com/read/28651260/the-cdd-system-in-computed-tomographic-diagnosis-of%C3%A2-diverticular-disease
#2
Daniel Pustelnik, Fabian Henry Jürgen Elsholtz, Christian Bojarski, Bernd Hamm, Stefan Markus Niehues
Purpose This overview sums up the Classification of Diverticular Disease (CDD) with regard to its application in computed tomographic diagnosis and briefly recapitulates its targeted advantages over preliminary systems. Primarily, application of the CDD in computed tomography diagnostics is described. Differences with respect to the categories of the older systems are pointed out on the level of each CDD type using imaging examples. Materials and Methods The presented images are derived from our institute according to the S2k criteria...
June 26, 2017: RöFo: Fortschritte Auf Dem Gebiete der Röntgenstrahlen und der Nuklearmedizin
https://www.readbyqxmd.com/read/28651231/evaluation-of-in-vitro-anticancer-activity-of-1-8-cineole-containing-n-hexane-extract-of-callistemon-citrinus-curtis-skeels-plant-and-its-apoptotic-potential
#3
Sowndarya Sampath, Vidhya Veeramani, Gopal Shankar Krishnakumar, Udhayakumar Sivalingam, Suguna Lakshmi Madurai, Rose Chellan
Plants are the source of a variety of secondary metabolites, which are often used in the anticancer activity. Discovering new anticancer drug from herbal source is more important in both biological and pharmacological activities. Hence, the objective of this study is to identify the anticancer agent in Callistemon citrinus (Curtis) Skeels (CC) for the treatment of cancer. Very recently we have reported an increased antioxidant activity in the ethanolic and methanolic extracts (EE and ME) of CC but significantly reduced activity (rather increased cytotoxicity), in the n-hexane extract (HE)...
June 23, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28651203/carbon-coated-zno-mat-passivation-by-atomic-layer-deposited-hfo2-as-an-anode-material-for-lithium-ion-batteries
#4
Mi-Hee Jung
ZnO has had little consideration as an anode material in lithium-ion batteries compared with other transition-metal oxides due to its inherent poor electrical conductivity and large volume expansion upon cycling and pulverization of ZnO-based electrodes. A logical design and facile synthesis of ZnO with well-controlled particle sizes and a specific morphology is essential to improving the performance of ZnO in lithium-ion batteries. In this paper, a simple approach is reported that uses a cation surfactant and a chelating agent to synthesize three-dimensional hierarchical nanostructured carbon-coated ZnO mats, in which the ZnO mats are composed of stacked individual ZnO nanowires and form well-defined nanoporous structures with high surface areas...
June 20, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28651180/does-a-short-term-exposure-to-atrazine-provoke-cellular-senescence-in-chlamydomonas-reinhardtii
#5
Marta Esperanza, Magali Houde, Marta Seoane, Ángeles Cid, Carmen Rioboo
The aim of this study was to investigate the impacts and modes of action of a chemical and nutrient deprivation on the cellular senescence process of Chlamydomonas reinhardtii. Several molecular and cellular parameters related to senescence phenomena were monitored in C. reinhardtii cells exposed for 24h to a sublethal concentration (0.25μM) of the herbicide atrazine and in unexposed 96h cells in an early stationary phase of growth. All endpoints showed the same pattern of response between treatments, except for the intracellular level of calcium, where a significant increase was observed in 24h-exposed cultures compared to 24h-log controls...
June 19, 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/28651004/a-human-endogenous-retrovirus-derived-gene-that-can-contribute-to-oncogenesis-by-activating-the-erk-pathway-and-inducing-migration-and-invasion
#6
Cécile Lemaître, Jhen Tsang, Caroline Bireau, Thierry Heidmann, Marie Dewannieux
Endogenous retroviruses are cellular genes of retroviral origin captured by their host during the course of evolution and represent around 8% of the human genome. Although most are defective and transcriptionally silenced, some are still able to generate retroviral-like particles and proteins. Among these, the HERV-K(HML2) family is remarkable since its members have amplified relatively recently and many of them still have full length coding genes. Furthermore, they are induced in cancers, especially in melanoma, breast cancer and germ cell tumours, where viral particles, as well as the envelope protein (Env), can be detected...
June 26, 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28650978/a-study-of-ticks-and-tick-borne-livestock-pathogens-in-pakistan
#7
Shahid Karim, Khemraj Budachetri, Nabanita Mukherjee, Jaclyn Williams, Asma Kausar, Muhammad Jawadul Hassan, Steven Adamson, Scot E Dowd, Dmitry Apanskevich, Abdullah Arijo, Zia Uddin Sindhu, Muhammad Azam Kakar, Raja Muhammad Dilpazir Khan, Shafiq Ullah, Muhammad Sohail Sajid, Abid Ali, Zafar Iqbal
BACKGROUND: As obligate blood-feeding arthropods, ticks transmit pathogens to humans and domestic animals more often than other arthropod vectors. Livestock farming plays a vital role in the rural economy of Pakistan, and tick infestation causes serious problems with it. However, research on tick species diversity and tick-borne pathogens has rarely been conducted in Pakistan. In this study, a systematic investigation of the tick species infesting livestock in different ecological regions of Pakistan was conducted to determine the microbiome and pathobiome diversity in the indigenous ticks...
June 26, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28650963/a-new-mechanistic-approach-for-the-further-development-of-a-population-with-established-size-bimodality
#8
Lisa Heermann, Donald L DeAngelis, Jost Borcherding
Usually, the origin of a within-cohort bimodal size distribution is assumed to be caused by initial size differences or by one discrete period of accelerated growth for one part of the population. The aim of this study was to determine if more continuous pathways exist allowing shifts from the small to the large fraction within a bimodal age-cohort. Therefore, a Eurasian perch population, which had already developed a bimodal size-distribution and had differential resource use of the two size-cohorts, was examined...
2017: PloS One
https://www.readbyqxmd.com/read/28650951/development-and-progression-of-a-lamellar-macular-hole-with-lamellar-hole-associated-epiretinal-proliferation
#9
Denise Compera, Matteo G Cereda, Ricarda G Schumann, Ferdinando Bottoni
PURPOSE: To describe evolution and progression of a lamellar macular hole (LMH) with "lamellar hole-associated epiretinal proliferation (LHEP)" using spectral-domain optical coherence tomography and fundus autofluorescence. METHODS: Observational case report. RESULTS: We report on a 63-year-old male patient demonstrating a complete history of LMH development with LHEP occurring during a follow-up period of 8 years. Presenting with a normal foveal contour and attached posterior vitreous at first visit, an LMH developed shortly after incomplete posterior vitreous detachment with vitreopapillary adhesion...
June 23, 2017: Retinal Cases & Brief Reports
https://www.readbyqxmd.com/read/28650927/dexamethasone-prevents-lipopolysaccharide-induced-epithelial-barrier-dysfunction-in-rat-ileum
#10
Aline Barbosa Ribeiro, Humberto Giusti, Ana Paula Trevelin Souza, Celso Rodrigues Franci, Rafael Simone Saia
Inflammatory mediators have been postulated as elementary inducing factors to the disruption of the intestinal tight junctions (TJ) and consequently, gut permeability and bacterial translocation. Corticosteroids are considered the mainstay in the treatment of septic shock; however the impact of this therapy on the intestinal epithelial barrier dysfunction during septic shock remains unknown. Our aims were to demonstrate the role of low dexamethasone (DEX) doses in modulation of the inflammatory response, as well as the expression and the arrangement of TJ proteins in endotoxemic rats...
June 23, 2017: Shock
https://www.readbyqxmd.com/read/28650897/ultrasonographic-evaluation-of-the-urethral-rhabdosphincter-morphology-in-female-patients-with-urodynamic-stress-incontinence
#11
Dimitrios Zacharakis, Themos Grigoriadis, Eleni Pitsouni, Ekaterini Domali, Athanasios Protopapas, Stavros Athanasiou
OBJECTIVES: During the last 2 decades, ultrasonography has been proposed for the morphologic evaluation of the urethral rhabdosphincter. The aims of this study were to evaluate the feasibility of a simple sonographic technique for the assessment of the urethral rhabdosphincter morphology by using a 2-dimensional (2D) transvaginal transducer and to evaluate any associations between the sonographic parameters of rhabdosphincter morphology with the presence of urodynamic stress incontinence (USI)...
July 2017: Female Pelvic Medicine & Reconstructive Surgery
https://www.readbyqxmd.com/read/28650784/sem-analysis-of-residual-dentin-surface-in-primary-teeth-using-different-chemomechanical-caries-removal-agents
#12
Rachna Thakur, Sandya Devi S Patil, Anil Kush, K Madhu
BACKGROUND: The purpose of this in vitro study was to analyze the residual dentinal surfaces following caries removal using two chemomechanical methods (Papacarie Duo and Carie Care), by scanning electron microscopy (SEM). STUDY DESIGN: Twenty extracted primary molars with active occlusal carious lesions were randomly assigned two groups depending on the CMCR agent used for the caries excavation - Group 1 - with Papacarie Duo and Group - 2 with Carie Care. After the caries excavation, the specimens were subjected to SEM analysis...
2017: Journal of Clinical Pediatric Dentistry
https://www.readbyqxmd.com/read/28650763/posttraumatic-retronychia-of-the-foot-with-clinical-and-ultrasound-correlation
#13
Mariana Pizarro, Nicolás Pieressa, Ximena Wortsman
Retronychia, the posterior embedding of the nail bed into the proximal nail fold, can be a complex clinical dermatologic diagnosis that may mimic other inflammatory ungual diseases or tumors of the nail. It has been related to a history of severe systemic conditions that secondarily affect the nail matrix and is commonly associated with onychomadesis. We present a case of retronychia of the foot with a purely traumatic origin, nonconcomitant with onychomadesis, that was diagnosed by color Doppler ultrasound in a long-time practitioner of martial arts who was otherwise healthy...
May 2017: Journal of the American Podiatric Medical Association
https://www.readbyqxmd.com/read/28650698/the-rock-isoforms-differentially-regulate-the-morphological-characteristics-of-carcinoma-cells
#14
Rachel J Jerrell, Mitchell J Leih, Aron Parekh
Rho-associated kinase (ROCK) activity drives cell migration via actomyosin contractility. During invasion, individual cancer cells can transition between two modes of migration, mesenchymal and amoeboid. Changes in ROCK activity can cause a switch between these migration phenotypes which are defined by distinct morphologies. However, recent studies have shown that the ROCK isoforms are not functionally redundant as previously thought. Therefore, it is unclear whether the ROCK isoforms play different roles in regulating migration phenotypes...
June 26, 2017: Small GTPases
https://www.readbyqxmd.com/read/28650694/investigation-of-the-developmental-potential-and-developmental-kinetics-of-bovine-parthenogenetic-and-somatic-cell-nuclear-transfer-embryos-using-a-time-lapse-monitoring-system
#15
Min-Jee Park, Eun-Young Kim, Man-Jong Kang, Jun-Beom Lee, Chang-Jin Jeong, Se-Pill Park
A time-lapse monitoring system has predictive value for selecting good-quality embryos with the highest implantation potential. Using this new tool, we investigated the developmental potential and developmental kinetics of bovine parthenogenetic (PA) and two types of somatic cell nuclear transfer (NT) embryos. Bovine non-transgenic ear cells (bECs) or transgenic cells (bTGCs) were used as donor cells. The cleavage and blastocyst development rates did not significantly differ among the PA, NT-bEC, and NT-bTGC groups, and first cleavage occurred an average of 19...
June 26, 2017: Cellular Reprogramming
https://www.readbyqxmd.com/read/28650660/ph-triggered-recovery-and-reuse-of-thiolated-poly-acryl-acid-functionalized-gold-nanoparticles-with-applications-in-colloidal-catalysis
#16
Siyam M Ansar, Benjamin D Fellows, Patrick Mispireta, O Thompson Mefford, Christopher L Kitchens
Thiolated poly(acrylic acid) (PAA-SH) functionalized gold nanoparticles were explored as a colloidal catalyst with potential application as a recoverable catalyst where the PAA provides pH-responsive dispersibility and phase transfer capability between aqueous and organic media. This system demonstrates complete nanoparticle recovery and redispersion over multiple reaction cycles without changes in nanoparticle morphology or reduction in conversion. The catalytic activity (rate constant) was reduced in subsequent reactions when recovery by aggregation was employed, despite unobservable changes in morphology or dispersability...
June 26, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28650647/highly-efficient-and-stable-sn-rich-perovskite-solar-cells-by-introducing-bromine
#17
Seojun Lee, Dong-Won Kang
Compositional engineering of recently-arising methylammonium (MA) lead (Pb) halide based perovskites is an essential approach for finding better perovskite compositions to resolve still remaining issues of toxic Pb, and long-term instability, etc. In this work, we carried out crystallographic, morphological, optical, and photovoltaic characterization of compositional MASn0.6Pb0.4I3-xBrx by gradually introducing bromine (Br) into parental Pb-Sn binary perovskite (MASn0.6Pb0.4I3) to elucidate its function in Sn-rich (Sn : Pb = 6 : 4) perovskites...
June 26, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28650641/a-discovery-of-strong-metal-support-bonding-in-nano-engineered-au-fe3o4-dumbbell-like-nanoparticles-by-in-situ-transmission-electron-microscopy
#18
Chang Wan Han, Tej Choksi, Cory Adam Milligan, Paulami Majumdar, Michael J Manto, Yanran Cui, Xiahan Sang, Raymond R Unocic, Dmitry Y Zemlyanov, Chao Wang, Fabio H Ribeiro, Jeffrey Greeley, Volkan Ortalan
The strength of metal-support bonding in heterogeneous catalysts determines their thermal stability, therefore, a tremendous amount of effort has been expended to understand metal-support interactions. Herein, we report the discovery of an anomalous "strong metal-support bonding" between gold nanoparticles and "nano-engineered" Fe3O4 substrates by in-situ microscopy. During in-situ vacuum annealing of Au-Fe3O4 dumbbell-like nanoparticles, synthesized by the epitaxial growth of nano-Fe3O4 on Au nanoparticles, the gold nanoparticles transform into the gold thinfilms and wet the surface of nano-Fe3O4, as the surface reduction of nano-Fe3O4 proceeds...
June 26, 2017: Nano Letters
https://www.readbyqxmd.com/read/28650624/precise-steric-control-over-2d-versus-3d-self-assembly-of-antimony-iii-alkoxide-cages-through-strong-secondary-bonding-interactions
#19
Shiva Moaven, Jingze Yu, Jason Yasin, Daniel K Unruh, Anthony F Cozzolino
Antimony(III) alkoxide cages were designed as building blocks for predictable supramolecular self-assembly. Supramolecular synthons featuring two Sb···O secondary bonding interactions (SBIs), each SBI stronger than 30 kJ/mol, were used to drive the formation of the supramolecular architectures. Judicious choice of pendant groups provided predictable control over the formation of self-assembled 3D columnar helices, which crystallized with hollow morphologies, or a self-assembled 2D bilayer. The Sb-O stretching frequency provides a spectroscopic signature of Sb···O SBI formation...
June 26, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/28650484/insertional-mutagenesis-identifies-drivers-of-a-novel-oncogenic-pathway-in-invasive-lobular-breast-carcinoma
#20
Sjors M Kas, Julian R de Ruiter, Koen Schipper, Stefano Annunziato, Eva Schut, Sjoerd Klarenbeek, Anne Paulien Drenth, Eline van der Burg, Christiaan Klijn, Jelle J Ten Hoeve, David J Adams, Marco J Koudijs, Jelle Wesseling, Micha Nethe, Lodewyk F A Wessels, Jos Jonkers
Invasive lobular carcinoma (ILC) is the second most common breast cancer subtype and accounts for 8-14% of all cases. Although the majority of human ILCs are characterized by the functional loss of E-cadherin (encoded by CDH1), inactivation of Cdh1 does not predispose mice to develop mammary tumors, implying that mutations in additional genes are required for ILC formation in mice. To identify these genes, we performed an insertional mutagenesis screen using the Sleeping Beauty transposon system in mice with mammary-specific inactivation of Cdh1...
June 26, 2017: Nature Genetics
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