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colon cancer, EMT

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https://www.readbyqxmd.com/read/29163688/foxm1-is-associated-with-metastasis-in-colorectal-cancer-through-induction-of-the-epithelial-mesenchymal-transition
#1
Bao-Ying Fei, Xujun He, Jie Ma, Mei Zhang, Rui Chai
The aim of the present study was to investigate the role of forkhead box M1 (FoxM1) in epithelial-mesenchymal transition (EMT) and metastasis in colorectal cancer (CRC). Immunohistochemical assays were performed to detect FoxM1 and epithelial (E-) cadherin protein expression in 92 CRC, 61 colonic adenoma and 32 wild-type colonic tissue samples. Quantitative polymerase chain reaction (qPCR) assays were performed to determine the expression levels of FoxM1 and E-cadherin mRNAs in 30 CRC and adjacent normal mucosal tissues...
December 2017: Oncology Letters
https://www.readbyqxmd.com/read/29158781/hypothalamic-beta-endorphin-neurons-suppress-preneoplastic-and-neoplastic-lesions-development-in-1-2-dimethylhydrazine-induced-rat-colon-cancer-model
#2
Sengottuvelan Murugan, Yatee Dave, Ankush Rakhit, Dipak K Sarkar
In recent years, experimental studies demonstrated negative impacts of impaired body stress response on colonic pathologies. In this study, we tested if reducing body stress response by the use of β-endorphin (BEP) neuronal transplants in the hypothalamus suppresses pre-neoplastic and neoplastic lesions. Colon cancer was induced by injecting 1,2-dimethylhydrazine (DMH) for sixteen weeks in Sprague Dawley rats with BEP neuron transplants or control neuron transplants, and their colonic histopathologies, colon tissue levels of pro-inflammatory cytokines and epithelial-mesenchymal transition (EMT) proteins and splenic levels of cytotoxic proteins were measured...
2017: Journal of Cancer
https://www.readbyqxmd.com/read/29156750/somatic-mutations-in-cdh1-and-ctnnb1-in-primary-carcinomas-at-13-anatomic-sites
#3
Evan L Busch, Jason L Hornick, Renato Umeton, Adem Albayrak, Neal I Lindeman, Laura E MacConaill, Elizabeth P Garcia, Matthew Ducar, Timothy R Rebbeck
Metastases are involved in most cancer deaths. Evidence has suggested that cancer cell detachment from primary tumors might occur largely via the mechanism of epithelial-mesenchymal transition (EMT) activated by epigenetic events, but data addressing other possible triggers of detachment, particularly genetic mutations, have been limited. Using the Profile study of cancer genomics at Dana-Farber Cancer Institute, we examined somatic mutations in the EMT genes CDH1 in 5,106 primary carcinomas and CTNNB1 in 7,578 primary carcinomas across 13 anatomic sites: urinary bladder, breast, colon/rectum, endometrium, esophagus, kidney, lung, ovary, pancreas, prostate, skin (non-melanoma), stomach, and thyroid...
October 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/29155818/snail1-mediated-downregulation-of-foxa-proteins-facilitates-the-inactivation-of-transcriptional-enhancer-elements-at-key-epithelial-genes-in-colorectal-cancer-cells
#4
Sabine Jägle, Hauke Busch, Vivien Freihen, Sven Beyes, Monika Schrempp, Melanie Boerries, Andreas Hecht
Phenotypic conversion of tumor cells through epithelial-mesenchymal transition (EMT) requires massive gene expression changes. How these are brought about is not clear. Here we examined the impact of the EMT master regulator SNAIL1 on the FOXA family of transcription factors which are distinguished by their particular competence to induce chromatin reorganization for the activation of transcriptional enhancer elements. We show that the expression of SNAIL1 and FOXA genes is anticorrelated in transcriptomes of colorectal tumors and cell lines...
November 20, 2017: PLoS Genetics
https://www.readbyqxmd.com/read/29151941/zeb1-promotes-oxaliplatin-resistance-through-the-induction-of-epithelial-mesenchymal-transition-in-colon-cancer-cells
#5
Cao Guo, Junli Ma, Ganlu Deng, Yanlin Qu, Ling Yin, Yiyi Li, Ying Han, Changjing Cai, Hong Shen, Shan Zeng
Background: Oxaliplatin (OXA) chemotherapy is widely used in the clinical treatment of colon cancer. However, chemo-resistance is still a barrier to effective chemotherapy in cases of colon cancer. Accumulated evidence suggests that the epithelial mesenchymal transition (EMT) may be a critical factor in chemo-sensitivity. The present study investigated the effects of Zinc finger E-box binding homeobox 1 (ZEB1) on OXA-sensitivity in colon cancer cells. Method: ZEB1expression and its correlation with clinicopathological characteristics were analyzed using tumor tissue from an independent cohort consisting of 118 colon cancer (CC) patients who receiving OXA-based chemotherapy...
2017: Journal of Cancer
https://www.readbyqxmd.com/read/29145973/tak-ing-aim-at-chemoresistance-the-emerging-role-of-map3k7-as-a-target-for-cancer-therapy
#6
Raffaela Santoro, Carmine Carbone, Geny Piro, Paul J Chiao, Davide Melisi
Cellular drug resistance remains the main obstacle to the clinical efficacy of cancer chemotherapy. Alterations in key pathways regulating cell cycle checkpoints, apoptosis and Epithelial to Mesenchymal Transition (EMT), such as the Mitogen-activated protein kinase (MAPK) pathway, appear to be closely associated to cancer chemoresistance. Transforming growth factor-β (TGF-β)- activated kinase 1 (TAK1, also known as MAP3K7) is a serine/threonine kinase in the mitogen-activated protein kinase (MAP3K) family...
November 2017: Drug Resistance Updates: Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy
https://www.readbyqxmd.com/read/29138850/role-of-mitochondrial-function-in-the-invasiveness-of-human-colon-cancer-cells
#7
Chen-Sung Lin, Li-Tzu Liu, Liang-Hung Ou, Siao-Cian Pan, Chia-I Lin, Yau-Huei Wei
We investigated the role of mitochondrial function in the invasiveness of human colorectal cancer (CRC) cell lines, using paired primary SW480 and metastatic SW620 cells, and appraised the clinical relevance of the alteration of mtDNA copy number in 33 pairs of CRC specimens after surgical resection. Suppression of mitochondrial function was achieved by the exposure of cells to oligomycin A (OA) or by knockdown of mitochondrial transcriptional factor A (TFAM) to evaluate their effects on energy metabolism, reactive oxygen species, protein expression levels of epithelial-mesenchymal transition (EMT) markers and invasive activity of CRC cells...
November 9, 2017: Oncology Reports
https://www.readbyqxmd.com/read/29137281/hic-5-regulates-epithelial-to-mesenchymal-transition-in-ovarian-cancer-cells-in-a-tgf%C3%AE-1-independent-manner
#8
Razan Sheta, Zhi-Qiang Wang, Magdalena Bachvarova, Marie Plante, Jean Gregoire, Marie-Claude Renaud, Alexandra Sebastianelli, Stephane Gobeil, Chantale Morin, Elizabeth Macdonald, Barbara Vanderhyden, Dimcho Bachvarov
The molecular basis of epithelial ovarian cancer (EOC) dissemination is still poorly understood. We have previously identified the hydrogen peroxide-inducible clone-5 (Hic-5) gene as hypomethylated in high-grade (HG) serous EOC tumors, compared to normal ovarian tissues. Hic-5 is a focal adhesion scaffold protein and has been primarily studied for its role as a key mediator of TGF-β-induced epithelial-to-mesenchymal transition (EMT) in epithelial cells of both normal and malignant origin; however, its role in EOC has been never investigated...
October 10, 2017: Oncotarget
https://www.readbyqxmd.com/read/29128131/the-clinical-and-biological-roles-of-transforming-growth-factor-beta-in-colon-cancer-stem-cells-a-systematic-review
#9
REVIEW
Anna Chruścik, Vinod Gopalan, Alfred King-Yin Lam
BACKGROUND: Transforming growth factor beta (TGF-β) is a multipurpose cytokine, which plays a role in many cellular functions such as proliferation, differentiation, migration, apoptosis, cell adhesion and regulation of epithelial to mesenchymal transition. Despite many studies having observed the effect that TGF-β plays in colorectal cancer, its role in the colorectal stem cell population has not been widely observed. METHOD: This systematic review will analyse the role of TGF-β in the stem cell population of colorectal cancer...
November 8, 2017: European Journal of Cell Biology
https://www.readbyqxmd.com/read/29117943/fbxo31-suppresses-gastric-cancer-emt-by-targeting-snail1-for-proteasomal-degradation
#10
Shuiyan Zou, Cunying Ma, Fenghua Yang, Xia Xu, Jihui Jia, Zhifang Liu
The F-box protein FBXO31, a component of the Skp1/Cul1/F-box (SCF) E3 ubiquitin ligase complex, plays an important regulatory role in neuronal development, stress response and tumorigenesis. Our recent report indicates that FBXO31 functions as a tumor suppressor in gastric cancer and the loss of FBXO31 protein is associated with a higher malignant phenotype and poorer prognosis. However, little is known about the underlying mechanism. In this study, FBXO31 inhibits gastric cancer progression by suppressing the epithelial-mesenchymal transition (EMT)...
November 8, 2017: Molecular Cancer Research: MCR
https://www.readbyqxmd.com/read/29115546/influence-of-the-twist-gene-on-the-invasion-and-metastasis-of-colon-cancer
#11
Duowei Wang, Bikash Rai, Feng Qi, Tong Liu, Jinmiao Wang, Xiaodong Wang, Bozhao Ma
The present study investigated the role of the Twist gene in epithelial-mesenchymal transition (EMT) and its effects on the invasion and metastasis of malignant tumors. In vitro, we transfected SW480, HCT116 and HT29 cells with recombinant plasmids, pTracer-CMV/BSD-Twist and pGenesil1.2-Twist-shRNA, to influence expression of Twist. The transfection efficacy of the plasmids in the cell lines was confirmed by flow cytometry. The relative mRNA and protein expression levels of Twist, E-cadherin and vimentin in the transfected cells were detected by RT-PCR and western blotting, respectively...
November 6, 2017: Oncology Reports
https://www.readbyqxmd.com/read/29109804/effects-of-tumor-necrosis-factor-%C3%AE-on-morphology-and-mechanical-properties-of-hct116-human-colon-cancer-cells-investigated-by-atomic-force-microscopy
#12
Huiqing Liu, Nan Wang, Zhe Zhang, Hongda Wang, Jun Du, Jilin Tang
Chronic inflammation orchestrates the tumor microenvironment and is strongly associated with cancer. Tumor necrosis factor-α (TNFα) is involved in tumor invasion and metastasis by inducing epithelial to mesenchymal transition (EMT). This process is defined by the loss of epithelial characteristics and gain of mesenchymal traits. The mechanisms of TNFα-induced EMT in cancer cells have been well studied. However, mechanical properties have not yet been probed. In this work, atomic force microscopy (AFM) was applied to investigate the morphology and mechanical properties of EMT in HCT116 human colon cancer cells...
2017: Scanning
https://www.readbyqxmd.com/read/29072916/comparative-proteomics-analysis-identifies-cdc42-cdc42bpa-signaling-as-prognostic-biomarker-and-therapeutic-target-for-colon-cancer-invasion
#13
Hui-Fang Hu, Wen Wen Xu, Yang Wang, Can-Can Zheng, Wei-Xia Zhang, Bin Li, Qing-Yu He
Metastasis is one of the major causes of treatment failure in the patients with colon cancer. The aim of our study is to find key proteins and pathways that drive invasion and metastasis in colon cancer. Eight rounds of selection of cancer cells invading through matrigel-coated chamber were performed to obtain highly invasive colon cancer sublines HCT116-I8 and RKO-I8. Stable Isotope Labeling by Amino Acids in Cell Culture technology was used to identify the differently expressed proteins, and the proteomics data were analyzed by ingenuity pathway analysis...
November 7, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/29069721/evolutionary-cancer-favoring-engineered-vaccinia-virus-for-metastatic-hepatocellular-carcinoma
#14
So Young Yoo, Su-Nam Jeong, Dae Hwan Kang, Jeong Heo
Engineered vaccinia virus-based therapy shows promising results in patients with advanced hepatocellular carcinoma, although a strategic virus design for the metastatic liver and the study of its efficacy in treating the cancer has not been well assessed. In this paper, we proposed a simple and strategic virus design for targeting metastatic hepatocellular carcinoma. We developed an evolutionary cancer-favoring engineered vaccinia virus (CVV, which is produced by repeated selective replication in cancerous tissues and then deleting viral thymidine kinase genes) and investigated its therapeutic effects on metastatic liver cancer...
September 22, 2017: Oncotarget
https://www.readbyqxmd.com/read/29050211/silibinin-attenuates-radiation-induced-intestinal-fibrosis-and-reverses-epithelial-to-mesenchymal-transition
#15
Joong Sun Kim, Na-Kyung Han, Sung-Ho Kim, Hae-June Lee
Radiotherapy is a common treatment for cancer patients, but its use is often restricted by the tolerance of normal tissue. As cancer patients live longer, delayed radiation effects on normal tissue have become a concern. Radiation-induced enteropathy, including inflammatory bowel disease and fibrosis, are major issues for long-term cancer survivors. To investigate whether silibinin attenuates delayed radiation-induced intestinal injury in mice, we focused on intestinal fibrotic changes. Silibinin improved delayed radiation injuries in mice in association with decreased collagen deposition within the intestines and deceased transforming growth factor (TGF)-β1 levels in the intestine and plasma...
September 19, 2017: Oncotarget
https://www.readbyqxmd.com/read/29029452/the-h3k27me3-demethylase-kdm6a-is-suppressed-in-breast-cancer-stem-like-cells-and-enables-the-resolution-of-bivalency-during-the-mesenchymal-epithelial-transition
#16
Joseph H Taube, Nathalie Sphyris, Kelsey S Johnson, Keighley N Reisenauer, Taylor A Nesbit, Robiya Joseph, Geraldine V Vijay, Tapasree R Sarkar, Neeraja A Bhangre, Joon Jin Song, Jeffrey T Chang, Min Gyu Lee, Rama Soundararajan, Sendurai A Mani
The deposition of the activating H3K4me3 and repressive H3K27me3 histone modifications within the same promoter, forming a so-called bivalent domain, maintains gene expression in a repressed but transcription-ready state. We recently reported a significantly increased incidence of bivalency following an epithelial-mesenchymal transition (EMT), a process associated with the initiation of the metastatic cascade. The reverse process, known as the mesenchymal-epithelial transition (MET), is necessary for efficient colonization...
September 12, 2017: Oncotarget
https://www.readbyqxmd.com/read/28981199/microrna-661-modulates-redox-and-metabolic-homeostasis-in-colon-cancer
#17
Marta Gómez de Cedrón, Rebeca Acín Pérez, Ruth Sánchez-Martínez, Susana Molina, Jesús Herranz, Jaime Feliu, Guillermo Reglero, Jose Antonio Enríquez, Ana Ramírez de Molina
Cancer cell survival and metastasis are dependent on metabolic reprogramming that is capable of increasing resistance to oxidative and energetic stress. Targeting these two processes can be crucial for cancer progression. Herein, we describe the role of microRNA-661 (miR661) as epigenetic regulator of colon cancer (CC) cell metabolism. miR661 induces a global increase in reactive oxygen species (ROS), specifically in mitochondrial superoxide anions (SO(-) ), which appears to be mediated by decreased carbohydrate metabolism and pentose phosphate pathway, and by a higher dependency on mitochondrial respiration...
October 5, 2017: Molecular Oncology
https://www.readbyqxmd.com/read/28974231/clca1-suppresses-colorectal-cancer-aggressiveness-via-inhibition-of-the-wnt-beta-catenin-signaling-pathway
#18
Xiaofen Li, Wangxiong Hu, Jiaojiao Zhou, Yanqin Huang, Jiaping Peng, Ying Yuan, Jiekai Yu, Shu Zheng
BACKGROUND: Chloride channel accessory 1 (CLCA1) belongs to the calcium-sensitive chloride conductance protein family, which is mainly expressed in the colon, small intestine and appendix. This study was conducted to investigate the functions and mechanisms of CLCA1 in colorectal cancer (CRC). METHODS: The CLCA1 protein expression level in CRC patients was evaluated by enzyme-linked immunosorbent assay (ELISA), immunohistochemistry (IHC), and western blotting analysis...
October 3, 2017: Cell Communication and Signaling: CCS
https://www.readbyqxmd.com/read/28956065/the-peritoneal-soil-for-a-cancerous-seed-a-comprehensive-review-of-the-pathogenesis-of-intraperitoneal-cancer-metastases
#19
REVIEW
Justyna Mikuła-Pietrasik, Paweł Uruski, Andrzej Tykarski, Krzysztof Książek
Various types of tumors, particularly those originating from the ovary and gastrointestinal tract, display a strong predilection for the peritoneal cavity as the site of metastasis. The intraperitoneal spread of a malignancy is orchestrated by a reciprocal interplay between invading cancer cells and resident normal peritoneal cells. In this review, we address the current state-of-art regarding colonization of the peritoneal cavity by ovarian, colorectal, pancreatic, and gastric tumors. Particular attention is paid to the pro-tumoral role of various kinds of peritoneal cells, including mesothelial cells, fibroblasts, adipocytes, macrophages, the vascular endothelium, and hospicells...
September 27, 2017: Cellular and Molecular Life Sciences: CMLS
https://www.readbyqxmd.com/read/28955947/snail1-expression-in-human-colon-cancer-dld-1-cells-confers-invasive-properties-without-n-cadherin-expression
#20
Shoko Tanaka, Wakako Kobayashi, Misako Haraguchi, Kiyohide Ishihata, Norifumi Nakamura, Masayuki Ozawa
The epithelial-mesenchymal transition (EMT) is a fundamental characteristic of carcinoma cells. EMT is generally associated with a change in cellular morphology from cobblestone to spindle shape, reduced expression of epithelial markers such as E-cadherin, and enhanced expression of mesenchymal markers such as N-cadherin. This EMT-associated reciprocal expression of E-cadherin and N-cadherin has been called the "cadherin switch". Downregulation of E-cadherin enables cells to dissociate from colonies while upregulation of N-cadherin is associated with increased invasiveness...
December 2016: Biochemistry and Biophysics Reports
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