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https://www.readbyqxmd.com/read/29346439/interspecies-diversity-of-chloride-channel-regulators-calcium-activated-3-genes
#1
Lars Mundhenk, Nancy A Erickson, Nikolai Klymiuk, Achim D Gruber
Members of the chloride channel regulators, calcium-activated (CLCA) family, have been implicated in diverse biomedical conditions, including chronic inflammatory airway diseases such as asthma, chronic obstructive pulmonary disease, and cystic fibrosis, the activation of macrophages, and the growth and metastatic spread of tumor cells. Several observations, however, could not be repeated across species boundaries and increasing evidence suggests that select CLCA genes are particularly prone to dynamic species-specific evolvements...
2018: PloS One
https://www.readbyqxmd.com/read/29346430/estimation-of-inhalation-flow-profile-using-audio-based-methods-to-assess-inhaler-medication-adherence
#2
Terence E Taylor, Helena Lacalle Muls, Richard W Costello, Richard B Reilly
Asthma and chronic obstructive pulmonary disease (COPD) patients are required to inhale forcefully and deeply to receive medication when using a dry powder inhaler (DPI). There is a clinical need to objectively monitor the inhalation flow profile of DPIs in order to remotely monitor patient inhalation technique. Audio-based methods have been previously employed to accurately estimate flow parameters such as the peak inspiratory flow rate of inhalations, however, these methods required multiple calibration inhalation audio recordings...
2018: PloS One
https://www.readbyqxmd.com/read/29344664/crucial-role-of-ox40-ox40l-signaling-in-a-murine-model-of-asthma
#3
Wei Lei, Daxiong Zeng, Gaoqin Liu, Yehan Zhu, Jiajia Wang, Hongya Wu, Junhong Jiang, Jianan Huang
The aim of the present study was to explore the roles of OX40/OX40 ligand (OX40L) signaling and OX40+ T cells in ovalbumin (OVA)‑induced mouse asthma model. Asthma was induced by OVA exposure and subsequent co‑treatment with OX40L protein, neutralizing anti‑OX40L blocking antibody, OX40+ T cells or PBS. The protein expression levels of interleukin (IL)‑4, IL‑6, IL‑13, IL‑17, tumor necrosis factor (TNF)‑α and interferon (IFN)‑γ in bronchoalveolar lavage fluid (BALF) were examined using murine cytokine‑specific ELISA...
January 18, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29343554/biased-generation-and-in-situ-activation-of-lung-tissue-resident-memory-cd4-t-cells-in-the-pathogenesis-of-allergic-asthma
#4
Damian L Turner, Monica Goldklang, Filip Cvetkovski, Daniel Paik, Jordis Trischler, Josselyn Barahona, Minwei Cao, Ronak Dave, Nicole Tanna, Jeanine M D'Armiento, Donna L Farber
Asthma is a chronic inflammatory disease mediated by allergen-specific CD4 T cells that promote lung inflammation through recruitment of cellular effectors into the lung. A subset of lung T cells can persist as tissue-resident memory T cells (TRMs) following infection and allergen induction, although the generation and role of TRM in asthma persistence and pathogenesis remain unclear. In this study, we used a mouse model of chronic exposure to intranasal house dust mite (HDM) extract to dissect how lung TRMs are generated and function in the persistence and pathogenesis of allergic airway disease...
January 17, 2018: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/29342458/glucocorticoids-modulate-th1-and-th2-responses-in-asthmatic-mouse-models-by-inhibition-of-notch1-signaling
#5
Chengping Hu, Zhifang Li, Juntao Feng, Yongjun Tang, Ling Qin, Xinyue Hu, Yan Zhang, Ruoxi He
BACKGROUND: Notch1 has been linked to the pathogenesis of asthma due to its contribution on Th1/Th2 imbalance. γ-Secretase inhibitor (GSI) acts as an effective blocker of Notch1 signaling. Glucocorticoids (GCs) are the most effective anti-inflammatory drugs for asthma. The present study investigated the involvement of the Notch1 pathway in the anti-inflammatory effect of GCs and its association with Th1/Th2 balance. METHODS: The asthma model was established in BALB/c mice by sensitization with ovalbumin (OVA)...
January 18, 2018: International Archives of Allergy and Immunology
https://www.readbyqxmd.com/read/29339735/phenotype-and-functional-features-of-human-telomerase-reverse-transcriptase-immortalized-human-airway-smooth-muscle-cells-from-asthmatic-and-non-asthmatic-donors
#6
J K Burgess, A Ketheson, A Faiz, K A Limbert Rempel, B G Oliver, J P T Ward, A J Halayko
Asthma is an obstructive respiratory disease characterised by chronic inflammation with airway hyperresponsiveness. In asthmatic airways, there is an increase in airway smooth muscle (ASM) cell bulk, which differs from non-asthmatic ASM in characteristics. This study aimed to assess the usefulness of hTERT immortalisation of human ASM cells as a research tool. Specifically we compared proliferative capacity, inflammatory mediator release and extracellular matrix (ECM) production in hTERT immortalised and parent primary ASM cells from asthmatic and non-asthmatic donors...
January 16, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29339659/fc%C3%AE-r-ros-ck2%C3%AE-is-the-key-inducer-of-nf-%C3%AE%C2%BAb-activation-in-a-murine-model-of-asthma
#7
June-Mo Kim, Hae-Kyoung Kim, Yoo-Na Im, Yun-Soo Bae, Suhn-Young Im, Hern-Ku Lee
BACKGROUND: The transcription factor nuclear factor (NF)-κB plays a pivotal role in the development of allergic airway inflammation. However, the mechanism of NF-κB activation in asthma remains to be elucidated. METHODS: CK2α activation was assessed by CK2α phosphorylation and protein expression. Airway levels of histamine and cytokines were determined by ELISA. We used 2 (active and passive) forms of allergic pulmonary inflammation models. In the active form, the animals were immunized with ovalbumin (OVA) intraperitoneally, followed by an airway challenge with OVA...
January 17, 2018: International Archives of Allergy and Immunology
https://www.readbyqxmd.com/read/29339225/metabolism-and-pharmacokinetics-of-a-potent-n-acylindole-antagonist-of-the-oxe-receptor-for-the-eosinophil-chemoattractant-5-oxo-6-8-11-14-eicosatetraenoic-acid-5-oxo-ete-in-rats-and-monkeys
#8
Chintam Nagendra Reddy, Hussam Alhamza, Shishir Chourey, Qiuji Ye, Vivek Gore, Chantal Cossette, Sylvie Gravel, Irina Slobodchikova, Dajana Vuckovic, Joshua Rokach, William S Powell
We previously identified the indole 264 as a potent in vitro antagonist of the human OXE receptor that mediates the actions of the powerful eosinophil chemoattractant 5-oxo-ETE. No antagonists of this receptor are currently commercially available or are being tested in clinical studies. The lack of a rodent ortholog of the OXE receptor has hampered progress in this area because of the unavailability of commonly used mouse or rat animal models. In the present study, we examined the feasibility of using the cynomolgus monkey as an animal model to investigate the efficacy of orally administered 264 in future in vivo studies...
January 12, 2018: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/29337391/time-dependent-distinct-roles-of-toll-like-receptor-4-in-a-house-dust-mite-induced-asthma-mouse-model
#9
Takashi Ishii, Yuichi Niikura, Keigo Kurata, Masashi Muroi, Ken-Ichi Tanamoto, Takahide Nagase, Masahiro Sakaguchi, Naomi Yamashita
House dust mites (HDMs) are a common source of allergens that trigger both allergen-specific and innate immune responses in humans. Here we examined the effect of allergen concentration and the involvement of Toll-like receptor 4 (TLR4) in the process of sensitization to house dust mite allergens in an HDM-extract-induced asthma mouse model. Intranasal administration of HDM extract induced an immunoglobulin E response and eosinophilic inflammation in a dose-dependent manner from 2.5 to 30 μg/dose. In TLR4-knockout mice, the infiltration of eosinophils and neutrophils into the lung was decreased compared with that in wild-type mice in the early phase of inflammation (total of three doses)...
January 16, 2018: Scandinavian Journal of Immunology
https://www.readbyqxmd.com/read/29337345/sputum-cytology-during-late-phase-responses-to-inhalation-challenge-with-different-allergens
#10
Joana A Revez, Kieran J Killian, Paul M O'Byrne, Louis-Philippe Boulet, John W Upham, Gail M Gauvreau, Manuel A R Ferreira
BACKGROUND: In mouse models of allergic asthma, exposure to different allergens can trigger distinct inflammatory subtypes in the airways. We investigated if this observation extends to humans. METHODS: We compared the frequency of sputum inflammatory subtypes between mild allergic asthma subjects (n=129) exposed to different allergens in inhalation challenge tests. These tests were performed using a standardized protocol as part of clinical trials of experimental treatments for asthma, prior to drug randomization...
January 16, 2018: Allergy
https://www.readbyqxmd.com/read/29336706/family-caregivers-reported-nonadherence-to-the-controller-medication-of-asthma-in-children-in-casablanca-morocco-extent-and-associated-factors
#11
Sandra Ménard, Jalila Jbilou, Sophie Lauzier
OBJECTIVE: Recent statistics show a relatively high prevalence of asthma among Moroccan children and a weak control over their symptoms. To our knowledge, no research has been carried out to document adherence to the controller treatment in this population. This study aims 1) to assess the extent of children's nonadherence to the controller treatment of asthma in an urban region of Morocco as reported by a family caregiver, and 2) to identify the associated factors. METHODS: We conducted a cross-sectional study among caregivers of asthmatic children (2-12 years old) in different health and education facilities of Casablanca-Settat...
January 16, 2018: Journal of Asthma: Official Journal of the Association for the Care of Asthma
https://www.readbyqxmd.com/read/29332324/effects-of-macrolide-and-corticosteroid-in-neutrophilic-asthma-mouse-model
#12
Tai Joon An, Chin Kook Rhee, Ji Hye Kim, Young Rong Lee, Jin Young Chon, Chan Kwon Park, Hyoung Kyu Yoon
BACKGROUND: Asthma is a disease of chronic airway inflammation with heterogeneous features. Neutrophilic asthma is corticosteroid-insensitive asthma related to absence or suppression of TH2 process and increased TH1 and/or TH17 process. Macrolides are immunomodulatory drug that reduce airway inflammation, but their role in asthma is not fully known. The purpose of this study was to evaluate the role of macrolides in neutrophilic asthma and compare their effects with those of corticosteroids...
January 2018: Tuberculosis and Respiratory Diseases
https://www.readbyqxmd.com/read/29332017/insulin-resistance-and-lung-function-in-obese-asthmatic-pre-pubertal-children
#13
Paola Di Filippo, Alessandra Scaparrotta, Daniele Rapino, Tommaso de Giorgis, Marianna Immacolata Petrosino, Marina Attanasi, Sabrina Di Pillo, Francesco Chiarelli, Angelika Mohn
BACKGROUND: Recent findings have supposed that the underlying association between the increased prevalence of both asthma and obesity may be insulin resistance (IR). METHODS: Insulin and glucose serum levels were analyzed to calculate the homeostatic model assessment of insulin resistance (HOMA-IR) for IR in 98 pre-pubertal children. Lung function and allergy status evaluation were performed. The study population was divided into four groups: (1) obese asthmatic children (ObA); (2) normal-weight asthmatic children (NwA); (3) normal-weight non-asthmatic children (Nw) and (4) obese non-asthmatic children (Ob)...
January 26, 2018: Journal of Pediatric Endocrinology & Metabolism: JPEM
https://www.readbyqxmd.com/read/29331655/what-makes-it-so-difficult-for-nurses-to-coach-patients-in-shared-decision-making-a-process-evaluation
#14
Stephanie Anna Lenzen, Ramon Daniëls, Marloes Amantia van Bokhoven, Trudy van der Weijden, Anna Beurskens
BACKGROUND: Primary care nurses play a crucial role in coaching patients in shared decision making about goals and actions. This presents a challenge to practice nurses, who are frequently used to protocol-based working routines. Therefore, an approach was developed to support nurses to coach patients in shared decision making. OBJECTIVES: To investigate how the approach was implemented and experienced by practice nurses and patients. DESIGN: A process evaluation was conducted using quantitative and qualitative methods...
December 16, 2017: International Journal of Nursing Studies
https://www.readbyqxmd.com/read/29331313/predictors-of-exacerbation-risk-and-response-to-budesonide-in-patients-with-chronic-obstructive-pulmonary-disease-a-post-hoc-analysis-of-three-randomised-trials
#15
Mona Bafadhel, Stefan Peterson, Miguel A De Blas, Peter M Calverley, Stephen I Rennard, Kai Richter, Malin Fagerås
BACKGROUND: The peripheral blood eosinophil count might help identify those patients with chronic obstructive pulmonary disease (COPD) who will experience fewer exacerbations when taking inhaled corticosteroids (ICS). Previous post-hoc analyses have proposed eosinophil cutoffs that are both arbitrary and limited in evaluating complex interactions of treatment response. We modelled eosinophil count as a continuous variable to determine the characteristics that determine both exacerbation risk and clinical response to ICS in patients with COPD...
January 10, 2018: Lancet Respiratory Medicine
https://www.readbyqxmd.com/read/29331087/outdoor-pollen-is-a-trigger-of-child-and-adolescent-asthma-ed-presentations-a-systematic-review-and-meta-analysis
#16
REVIEW
Bircan Erbas, Mitra Jazayeri, Katrina A Lambert, Constance H Katelaris, Luke A Prendergast, Rachel Tham, M Jeannette Parrodi, Janet Davies, Ed Newbigin, Michael J Abramson, Shyamali C Dharmage
BACKGROUND: In the context of increased asthma exacerbations associated with climatic changes such as thunderstorm asthma, interest in establishing the link between pollen exposure and asthma hospital admissions has intensified. Here, we systematically reviewed and performed a meta-analysis of studies on pollen and ED attendance. METHODS: A search for studies with appropriate search strategy in Medline, Embase, Web of Science and CINAHL was conducted. Each study was assessed for quality and risk of bias...
January 13, 2018: Allergy
https://www.readbyqxmd.com/read/29331026/the-asthma-rhinitis-multimorbidity-is-associated-with-ige-polysensitization-in-adolescents-and-adults
#17
Valérie Siroux, Natalia Ballardini, Marion Soler, Christian Lupinek, Anne Boudier, Isabelle Pin, Jocelyne Just, Rachel Nadif, Josep M Anto, Erik Melen, Rudolf Valenta, Magnus Wickman, Jean Bousquet
BACKGROUND: Children with multimorbid asthma and rhinitis show IgE polysensitization to several allergen sources. This association remain poorly studied in adolescents and adults using defined allergen molecules. We investigated IgE sensitization patterns towards a broad panel of aeroallergen components in adults and adolescents with a focus on individuals with asthma and rhinitis multimorbidity. METHODS: IgE reactivity to 64 microarrayed aeroallergen molecules was determined with the MeDALL-chip in samples from the French EGEA study (n=840, age=40...
January 13, 2018: Allergy
https://www.readbyqxmd.com/read/29330321/osteopontin-promotes-protective-antigenic-tolerance-against-experimental-allergic-airway-disease
#18
Themis Alissafi, Evangelia Kourepini, Davina C M Simoes, Nikolaos Paschalidis, Maria Aggelakopoulou, Tim Sparwasser, Louis Boon, Hamida Hammad, Bart N Lambrecht, Vily Panoutsakopoulou
In the context of inflammation, osteopontin (Opn) is known to promote effector responses, facilitating a proinflammatory environment; however, its role during antigenic tolerance induction is unknown. Using a mouse model of asthma, we investigated the role of Opn during antigenic tolerance induction and its effects on associated regulatory cellular populations prior to disease initiation. Our experiments demonstrate that Opn drives protective antigenic tolerance by inducing accumulation of IFN-β-producing plasmacytoid dendritic cells, as well as regulatory T cells, in mediastinal lymph nodes...
January 12, 2018: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/29326334/clinical-and-inflammatory-phenotyping-by-breathomics-in-chronic-airway-diseases-irrespective-of-the-diagnostic-label
#19
Rianne de Vries, Yennece W F Dagelet, Pien Spoor, Erik Snoey, Patrick M C Jak, Paul Brinkman, Erica Dijkers, Simon K Bootsma, Fred Elskamp, Frans H C de Jongh, Eric G Haarman, Johannes C C M In 't Veen, Anke-Hilse Maitland-van der Zee, Peter J Sterk
Asthma and chronic obstructive pulmonary disease (COPD) are complex and overlapping diseases that include inflammatory phenotypes. Novel anti-eosinophilic/anti-neutrophilic strategies demand rapid inflammatory phenotyping, which might be accessible from exhaled breath.Our objective was to capture clinical/inflammatory phenotypes in patients with chronic airway disease using an electronic nose (eNose) in a training and validation set.This was a multicentre cross-sectional study in which exhaled breath from asthma and COPD patients (n=435; training n=321 and validation n=114) was analysed using eNose technology...
January 2018: European Respiratory Journal: Official Journal of the European Society for Clinical Respiratory Physiology
https://www.readbyqxmd.com/read/29324919/synchrotron-fourier-transform-infrared-maps-of-ovalbumin-induced-murine-chronic-allergic-airways-disease-correlation-with-conventional-histology
#20
Nadia Mazarakis, Jitraporn Vongsvivut, Keith R Bambery, Kenneth J Snibson, Simon G Royce, Tom C Karagiannis
Asthma is a chronic respiratory disease characterised by airway inflammation, remodeling and hyperresponsiveness. The ability to replicate these asthma traits in the well-established ovalbumin induced chronic model of allergic airways disease is an important tool for asthma research and preclinical drug development. Here, spectra derived from focal plane array and Synchrotron-Fourier transform infrared maps were used to analyse biochemical changes in lung tissue from an ovalbumin-induced murine chronic allergic airways disease model...
September 2017: Hellenic Journal of Nuclear Medicine
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