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https://www.readbyqxmd.com/read/29136834/comparative-proteomics-of-cancer-stem-cells-in-osteosarcoma-using-ultra-high-performance-liquid-chromatography-and-orbitrap-fusion-mass-spectrometer
#1
Zhihui Zhong, Sifeng Mao, Haifeng Lin, Jin-Ming Lin, Jianhua Lin
Osteosarcoma is the most common malignant tumour found in bones, and it has a poor prognosis. For improved therapy, it is significant to have a deep understanding of the proteomics changes in the cancer stem cells (CSCs) of osteosarcoma. Therefore, a comparative proteomics approach based on ultra-high-performance liquid chromatography coupled to an Orbitrap Fusion mass spectrometer (UHPLC-Orbitrap Fusion MS) was established to investigate the key molecular changes between CSCs and non-CSCs in human osteosarcoma HOS cells...
February 1, 2018: Talanta
https://www.readbyqxmd.com/read/29055790/%C3%AE-actinin-4-confers-radioresistance-coupled-invasiveness-in-breast-cancer-cells-through-akt-pathway
#2
Sejal Desai, Amlan Barai, Amirali B Bukhari, Abhijit De, Shamik Sen
Acquired radioresistance accompanied with increased metastatic potential is a major hurdle in effective radiotherapy of breast cancers. However, the nature of their inter-dependence and the underlying mechanism remains largely intangible. By employing radioresistant (RR) cell lines, we herein demonstrate that MCF-7 RR cells display phenotypic and molecular alterations evocative of epithelial to mesenchymal transition (EMT) with increased traction forces and membrane ruffling culminating in boosted invasiveness...
October 18, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29038887/mutations-in-inf2-may-be-associated-with-renal-histology-other-than-focal-segmental-glomerulosclerosis
#3
Anja K Büscher, Nora Celebi, Peter F Hoyer, Hanns-Georg Klein, Stefanie Weber, Julia Hoefele
BACKGROUND: In 2010, INF2 mutations were associated with autosomal-dominant focal segmental glomerulosclerosis (FSGS), clinically presenting with moderate proteinuria in adolescence. However, in the meantime, cases with more severe clinical courses have been described, including progression to end-stage renal disease (ESRD) during childhood. INF2 mutations in patients with isolated FSGS are clustered in exons 2 to 4, encoding the diaphanous inhibitory domain, involved in the regulation of the podocyte actin cytoskeleton...
October 6, 2017: Pediatric Nephrology: Journal of the International Pediatric Nephrology Association
https://www.readbyqxmd.com/read/28990106/identification-of-key-genes-for-diabetic-kidney-disease-using-biological-informatics-methods
#4
Fuzhe Ma, Tao Sun, Meiyan Wu, Wanning Wang, Zhonggao Xu
Diabetic kidney disease (DKD) is a common complication of diabetes, which is characterized by albuminuria, impaired glomerular filtration rate or a combination of the two. The aim of the present study was to identify the potential key genes involved in DKD progression and to subsequently investigate the underlying mechanism involved in DKD development. The array data of GSE30528 including 9 DKD and 13 control samples was downloaded from the Gene Expression Omnibus database. The differentially expressed genes (DEGs) in DKD glomerular and tubular kidney biopsy tissues were compared with normal tissues, and were analyzed using the limma package...
December 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28965411/next-generation-proteomic-pipeline-for-chromosome-based-proteomic-research-using-nextprot-and-gencode-databases
#5
Heeyoun Hwang, Gun Wook Park, Ji Yeong Park, Hyun Kyoung Lee, Ju Yeon Lee, Ji Eun Jeong, Sung-Kyu Robin Park, John R Yates, Kyung-Hoon Kwon, Young Mok Park, Hyoung-Joo Lee, Young-Ki Paik, Jin Young Kim, Jong Shin Yoo
Human Proteome Project aims to map all human proteins including missing proteins as well as proteoforms with post translational modifications, alternative splicing variants (ASVs), and single amino acid variants (SAAVs). neXtProt and Ensemble databases are usually used to provide curated information of human coding genes. However, in order to find these proteoforms, we (Chr #11 team) first introduce a streamlined pipeline using customized and concatenated neXtProt and GENCODE originated from Ensemble, with controlled false discovery rate (FDR)...
October 1, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28912799/triportheus-albus-cope-1872-in-the-blackwater-clearwater-and-whitewater-of-the-amazon-a-case-of-phenotypic-plasticity
#6
José D A Araújo, Andrea Ghelfi, Adalberto L Val
The Amazon basin includes 1000s of bodies of water, that are sorted according to their color in three types: blackwater, clearwater, and whitewater, which significantly differ in terms of their physicochemical parameters. More than 3,000 species of fish live in the rivers of the Amazon, among them, the sardine, Triportheus albus, which is one of the few species that inhabit all three types of water. The purpose of our study was to analyze if the gene expression of T. albus is determined by the different types of water, that is, if the species presents phenotypic plasticity to live in blackwater, clearwater, and whitewater...
2017: Frontiers in Genetics
https://www.readbyqxmd.com/read/28748856/the-short-isoform-of-nuclear-mitotic-apparatus-protein-1-functions-as-a-putative-tumor-suppressor
#7
Wang-Sen Qin, Jin Wu, Yang Chen, Fa-Cai Cui, Fu-Ming Zhang, Guan-Ting Lyu, Hong-Mei Zhang
BACKGROUND: Nuclear mitotic apparatus protein 1 (NuMA1) had been reported to produce three groups of isoforms categorized as long, middle, and short groups, of which short NuMA displayed distinct localization patterns compared to long and middle isoforms. However, the function of short NuMA was not clear in the progress of cancer formation. This study aimed to unveil the role of short NuMA in cancer pathogenesis. METHODS: The expression levels of short isoforms were explored in paired gastric carcinoma (GC) samples and different cell lines...
August 5, 2017: Chinese Medical Journal
https://www.readbyqxmd.com/read/28664914/lim-kinase-1-interacts-with-myosin-9-and-alpha-actinin-4-and-promotes-colorectal-cancer-progression
#8
Qing Liao, Rui Li, Rui Zhou, Zhihua Pan, Lijun Xu, Yanqing Ding, Liang Zhao
BACKGROUND: LIM kinase 1 (LIMK1) is a key regulator of the cytoskeletal organisation involved in cell proliferation and migration. Even though LIMK1 is frequently dysregulated in epithelial cancers, the role and mechanisms of LIMK1 in colorectal cancer (CRC) remains unclear. METHODS: Immunohistochemical analysis was performed to examine the expression and clinical significance of LIMK1 in CRC samples. Loss- and gain-of-function assay was performed to investigate the effects of aberrant expression on cellular biological behaviour of CRC cells in vitro and in vivo...
August 8, 2017: British Journal of Cancer
https://www.readbyqxmd.com/read/28629798/proteomic-analysis-of-mesenchymal-to-schwann-cell-transdifferentiation
#9
Anup D Sharma, Jayme Wiederin, Metin Uz, Pawel Ciborowski, Surya K Mallapragada, Howard E Gendelman, Donald S Sakaguchi
While transplantation of Schwann cells facilitates axon regeneration, remyelination and repair after peripheral nerve injury clinical use is limited by cell bioavailability. We posit that such limitation in cell access can be overcome by the use of autologous bone-marrow derived mesenchymal stem cells (MSCs). As MSCs can transdifferentiate to Schwann cell-phenotypes and accelerate nerve regeneration we undertook proteomic evaluation of the cells to uncover the protein contents that affects Schwann cell formulation...
June 17, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28581489/%C3%AE-actinin-4-promotes-metastasis-in-gastric-cancer
#10
Xin Liu, Kent-Man Chu
Metastasis increases the mortality rate of gastric cancer, which is the third leading cause of cancer-associated deaths worldwide. This study aims to identify the genes promoting metastasis of gastric cancer (GC). A human cell motility PCR array was used to analyze a pair of tumor and non-tumor tissue samples from a patient with stage IV GC (T3N3M1). Expression of the dysregulated genes was then evaluated in GC tissue samples (n=10) and cell lines (n=6) via qPCR. Expression of α-actinin-4 (ACTN4) was validated in a larger sample size (n=47) by qPCR, western blot and immunohistochemistry...
September 2017: Laboratory Investigation; a Journal of Technical Methods and Pathology
https://www.readbyqxmd.com/read/28579451/the-fifth-sense-mechanosensory-regulation-of-alpha-actinin-4-and-its-relevance-for-cancer-metastasis
#11
REVIEW
Dustin G Thomas, Douglas N Robinson
Metastatic cancer cells invading through dense tumor stroma experience internal and external forces that are sensed through a variety of mechanosensory proteins that drive adaptations for specific environments. Alpha-actinin-4 (ACTN4) is a member of the α-actinin family of actin crosslinking proteins that is upregulated in several types of cancers. It shares 86% protein similarity with α-actinin-1, another non-muscle ACTN isoform, which appears to have a more modest role, if any, in cancer progression. While they share regulatory mechanisms, such as phosphorylation, calcium binding, phosphatidyl inositol binding, and calpain cleavage, α-actinin-4 exhibits a unique mechanosensory regulation that α-actinin-1 does not...
November 2017: Seminars in Cell & Developmental Biology
https://www.readbyqxmd.com/read/28533408/rna-editing-of-slc22a3-drives-early-tumor-invasion-and-metastasis-in-familial-esophageal-cancer
#12
Li Fu, Yan-Ru Qin, Xiao-Yan Ming, Xian-Bo Zuo, Yu-Wen Diao, Li-Yi Zhang, Jiaoyu Ai, Bei-Lei Liu, Tu-Xiong Huang, Ting-Ting Cao, Bin-Bin Tan, Di Xiang, Chui-Mian Zeng, Jing Gong, Qiangfeng Zhang, Sui-Sui Dong, Juan Chen, Haibo Liu, Jian-Lin Wu, Robert Z Qi, Dan Xie, Li-Dong Wang, Xin-Yuan Guan
Like many complex human diseases, esophageal squamous cell carcinoma (ESCC) is known to cluster in families. Familial ESCC cases often show early onset and worse prognosis than the sporadic cases. However, the molecular genetic basis underlying the development of familial ESCC is mostly unknown. We reported that SLC22A3 is significantly down-regulated in nontumor esophageal tissues from patients with familial ESCC compared with tissues from patients with sporadic ESCCs. A-to-I RNA editing of the SLC22A3 gene results in its reduced expression in the nontumor esophageal tissues of familial ESCCs and is significantly correlated with lymph node metastasis...
June 6, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28485154/identification-of-prognostic-risk-factors-of-acute-lymphoblastic-leukemia-based-on-mrna-expression-profiling
#13
C Li, L Kuang, B Zhu, J Chen, X Wang, X Huang
We aim to identify prognosis risk factors in acute lymphoblastic leukemia (ALL). mRNA microarray data of adult ALL patients were downloaded from TCGA database, whose mRNAs were isolated from bone marrow aspirate fluid mononuclear cells. Then the differentially expressed genes (DEGs) between good and poor prognosis samples were screened. Following that, the sample dependency network was constructed based on the Pearson connection coefficients of DEGs in the samples. The prognosis-related genes were collected using logistic regression analysis...
May 9, 2017: Neoplasma
https://www.readbyqxmd.com/read/28385383/prognostic-significance-of-gene-amplification-of-actn4-in-stage-i-and-ii-oral-tongue-cancer
#14
T Kakuya, T Mori, S Yoshimoto, Y Watabe, N Miura, H Shoji, K Onidani, T Shibahara, K Honda
Despite complete resection of the early stage of oral tongue cancer by partial glossectomy, late cervical lymph node metastasis is frequently observed. Gene amplification of ACTN4 (protein name: actinin-4) is closely associated with the metastatic potential of various cancers. This retrospective study was performed to demonstrate the potential usefulness of ACTN4 gene amplification as a prognostic biomarker in patients with stage I/II oral tongue cancer. Fifty-four patients with stage I/II oral tongue cancer were enrolled retrospectively, in accordance with the reporting recommendations for tumour marker prognostic studies (REMARK) guidelines...
April 3, 2017: International Journal of Oral and Maxillofacial Surgery
https://www.readbyqxmd.com/read/28216224/targeted-proteomics-driven-verification-of-biomarker-candidates-associated-with-breast-cancer-aggressiveness
#15
Iva Procházková, Juraj Lenčo, Alena Fučíková, Jiří Dresler, Lenka Čápková, Roman Hrstka, Rudolf Nenutil, Pavel Bouchal
Breast cancer is the most common and molecularly relatively well characterized malignant disease in women, however, its progression to metastatic cancer remains lethal for 78% of patients 5years after diagnosis. Novel markers could identify the high risk patients and their verification using quantitative methods is essential to overcome genetic, inter-tumor and intra-tumor variability and translate novel findings into cancer diagnosis and treatment. We recently identified 13 proteins associated with estrogen receptor, tumor grade and lymph node status, the key factors of breast cancer aggressiveness, using untargeted proteomics...
May 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28111298/aerosol-from-tobacco-heating-system-2-2-has-reduced-impact-on-mouse-heart-gene-expression-compared-with-cigarette-smoke
#16
Justyna Szostak, Stéphanie Boué, Marja Talikka, Emmanuel Guedj, Florian Martin, Blaine Phillips, Nikolai V Ivanov, Manuel C Peitsch, Julia Hoeng
Experimental studies clearly demonstrate a causal effect of cigarette smoking on cardiovascular disease. To reduce the individual risk and population harm caused by smoking, alternative products to cigarettes are being developed. We recently reported on an apolipoprotein E-deficient (Apoe(-/-)) mouse inhalation study that compared the effects of exposure to aerosol from a candidate modified risk tobacco product, Tobacco Heating System 2.2 (THS2.2), and smoke from the reference cigarette (3R4F) on pulmonary and vascular biology...
March 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/27998979/%C3%AE-actinin-4-actn4-regulates-glucocorticoid-receptor-mediated-transactivation-and-transrepression-in-podocytes
#17
Xuan Zhao, Simran Khurana, Sharmistha Charkraborty, Yuqian Tian, John R Sedor, Leslie A Bruggman, Hung-Ying Kao
Glucocorticoids are a general class of steroids that possess renoprotective activity in glomeruli through their interaction with the glucocorticoid receptor. However, the mechanisms by which glucocorticoids ameliorate proteinuria and glomerular disease are not well understood. In this study, we demonstrated that α actinin 4 (ACTN4), an actin-cross-linking protein known to coordinate cytoskeletal organization, interacts with the glucocorticoid receptor (GR) in the nucleus of human podocytes (HPCs), a key cell type in the glomerulus critical for kidney filtration function...
February 3, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27874083/poly-l-ornithine-enhances-migration-of-neural-stem-progenitor-cells-via-promoting-%C3%AE-actinin-4-binding-to-actin-filaments
#18
Hongfei Ge, Anyong Yu, Jingyu Chen, Jichao Yuan, Yi Yin, Wangsheng Duanmu, Liang Tan, Yang Yang, Chuan Lan, Weixiang Chen, Hua Feng, Rong Hu
The recruitment of neural stem/progenitor cells (NSPCs) for brain restoration after injury is a promising regenerative therapeutic strategy. This strategy involves enhancing proliferation, migration and neuronal differentation of NSPCs. To date, the lack of biomaterials, which facilitate these processes to enhance neural regeneration, is an obstacle for the cell replacement therapies. Our previous study has shown that NSPCs grown on poly-L-ornithine (PO) could proliferate more vigorously and differentiate into more neurons than that on Poly-L-Lysine (PLL) and Fibronectin (FN)...
November 22, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27742266/proteomic-analysis-of-mucopolysaccharidosis-i-mouse-brain-with-two-dimensional-polyacrylamide-gel-electrophoresis
#19
Li Ou, Michael J Przybilla, Chester B Whitley
Mucopolysaccharidosis type I (MPS I) is due to deficiency of α-l-iduronidase (IDUA) and subsequent storage of undegraded glycosaminoglycans (GAG). The severe form of the disease, known as Hurler syndrome, is characterized by mental retardation and neurodegeneration of unknown etiology. To identify potential biomarkers and unveil the neuropathology mechanism of MPS I disease, two-dimensional polyacrylamide gel electrophoresis (PAGE) and nanoliquid chromatography-tandem mass spectrometry (nanoLC-MS/MS) were applied to compare proteome profiling of brains from MPS I and control mice (5-month old)...
January 2017: Molecular Genetics and Metabolism
https://www.readbyqxmd.com/read/27613525/a-two-gene-prognostic-classifier-for-early-stage-lung-squamous-cell-carcinoma-in-multiple-large-scale-and-geographically-diverse-cohorts
#20
Rintaro Noro, Teruhide Ishigame, Naomi Walsh, Kouya Shiraishi, Ana I Robles, Bríd M Ryan, Aaron J Schetter, Elise D Bowman, Judith A Welsh, Masahiro Seike, Akihiko Gemma, Vidar Skaug, Steen Mollerup, Aage Haugen, Jun Yokota, Takashi Kohno, Curtis C Harris
INTRODUCTION: There are no validated molecular methods that prospectively identify patients with surgically resected lung squamous cell carcinoma (SCC) at high risk for recurrence. By focusing on the expression of genes with known functions in development of lung SCC and prognosis, we sought to develop a robust prognostic classifier of early-stage lung SCC. METHODS: The expression of 253 genes selected by literature search was evaluated in microarrays from 107 stage I/II tumors...
January 2017: Journal of Thoracic Oncology
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