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https://www.readbyqxmd.com/read/28748856/the-short-isoform-of-nuclear-mitotic-apparatus-protein-1-functions-as-a-putative-tumor-suppressor
#1
Wang-Sen Qin, Jin Wu, Yang Chen, Fa-Cai Cui, Fu-Ming Zhang, Guan-Ting Lyu, Hong-Mei Zhang
BACKGROUND: Nuclear mitotic apparatus protein 1 (NuMA1) had been reported to produce three groups of isoforms categorized as long, middle, and short groups, of which short NuMA displayed distinct localization patterns compared to long and middle isoforms. However, the function of short NuMA was not clear in the progress of cancer formation. This study aimed to unveil the role of short NuMA in cancer pathogenesis. METHODS: The expression levels of short isoforms were explored in paired gastric carcinoma (GC) samples and different cell lines...
August 5, 2017: Chinese Medical Journal
https://www.readbyqxmd.com/read/28664914/lim-kinase-1-interacts-with-myosin-9-and-alpha-actinin-4-and-promotes-colorectal-cancer-progression
#2
Qing Liao, Rui Li, Rui Zhou, Zhihua Pan, Lijun Xu, Yanqing Ding, Liang Zhao
BACKGROUND: LIM kinase 1 (LIMK1) is a key regulator of the cytoskeletal organisation involved in cell proliferation and migration. Even though LIMK1 is frequently dysregulated in epithelial cancers, the role and mechanisms of LIMK1 in colorectal cancer (CRC) remains unclear. METHODS: Immunohistochemical analysis was performed to examine the expression and clinical significance of LIMK1 in CRC samples. Loss- and gain-of-function assay was performed to investigate the effects of aberrant expression on cellular biological behaviour of CRC cells in vitro and in vivo...
August 8, 2017: British Journal of Cancer
https://www.readbyqxmd.com/read/28629798/proteomic-analysis-of-mesenchymal-to-schwann-cell-transdifferentiation
#3
Anup D Sharma, Jayme Wiederin, Metin Uz, Pawel Ciborowski, Surya K Mallapragada, Howard E Gendelman, Donald S Sakaguchi
While transplantation of Schwann cells facilitates axon regeneration, remyelination and repair after peripheral nerve injury clinical use is limited by cell bioavailability. We posit that such limitation in cell access can be overcome by the use of autologous bone-marrow derived mesenchymal stem cells (MSCs). As MSCs can transdifferentiate to Schwann cell-phenotypes and accelerate nerve regeneration we undertook proteomic evaluation of the cells to uncover the protein contents that affects Schwann cell formulation...
June 17, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28581489/%C3%AE-actinin-4-promotes-metastasis-in-gastric-cancer
#4
Xin Liu, Kent-Man Chu
Metastasis increases the mortality rate of gastric cancer, which is the third leading cause of cancer-associated deaths worldwide. This study aims to identify the genes promoting metastasis of gastric cancer (GC). A human cell motility PCR array was used to analyze a pair of tumor and non-tumor tissue samples from a patient with stage IV GC (T3N3M1). Expression of the dysregulated genes was then evaluated in GC tissue samples (n=10) and cell lines (n=6) via qPCR. Expression of α-actinin-4 (ACTN4) was validated in a larger sample size (n=47) by qPCR, western blot and immunohistochemistry...
June 5, 2017: Laboratory Investigation; a Journal of Technical Methods and Pathology
https://www.readbyqxmd.com/read/28579451/the-fifth-sense-mechanosensory-regulation-of-alpha-actinin-4-and-its-relevance-for-cancer-metastasis
#5
REVIEW
Dustin G Thomas, Douglas N Robinson
Metastatic cancer cells invading through dense tumor stroma experience internal and external forces that are sensed through a variety of mechanosensory proteins that drive adaptations for specific environments. Alpha-actinin-4 (ACTN4) is a member of the α-actinin family of actin crosslinking proteins that is upregulated in several types of cancers. It shares 86% protein similarity with α-actinin-1, another non-muscle ACTN isoform, which appears to have a more modest role, if any, in cancer progression. While they share regulatory mechanisms, such as phosphorylation, calcium binding, phosphatidyl inositol binding, and calpain cleavage, α-actinin-4 exhibits a unique mechanosensory regulation that α-actinin-1 does not...
June 1, 2017: Seminars in Cell & Developmental Biology
https://www.readbyqxmd.com/read/28533408/rna-editing-of-slc22a3-drives-early-tumor-invasion-and-metastasis-in-familial-esophageal-cancer
#6
Li Fu, Yan-Ru Qin, Xiao-Yan Ming, Xian-Bo Zuo, Yu-Wen Diao, Li-Yi Zhang, Jiaoyu Ai, Bei-Lei Liu, Tu-Xiong Huang, Ting-Ting Cao, Bin-Bin Tan, Di Xiang, Chui-Mian Zeng, Jing Gong, Qiangfeng Zhang, Sui-Sui Dong, Juan Chen, Haibo Liu, Jian-Lin Wu, Robert Z Qi, Dan Xie, Li-Dong Wang, Xin-Yuan Guan
Like many complex human diseases, esophageal squamous cell carcinoma (ESCC) is known to cluster in families. Familial ESCC cases often show early onset and worse prognosis than the sporadic cases. However, the molecular genetic basis underlying the development of familial ESCC is mostly unknown. We reported that SLC22A3 is significantly down-regulated in nontumor esophageal tissues from patients with familial ESCC compared with tissues from patients with sporadic ESCCs. A-to-I RNA editing of the SLC22A3 gene results in its reduced expression in the nontumor esophageal tissues of familial ESCCs and is significantly correlated with lymph node metastasis...
June 6, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28485154/identification-of-prognostic-risk-factors-of-acute-lymphoblastic-leukemia-based-on-mrna-expression-profiling
#7
C Li, L Kuang, B Zhu, J Chen, X Wang, X Huang
We aim to identify prognosis risk factors in acute lymphoblastic leukemia (ALL). mRNA microarray data of adult ALL patients were downloaded from TCGA database, whose mRNAs were isolated from bone marrow aspirate fluid mononuclear cells. Then the differentially expressed genes (DEGs) between good and poor prognosis samples were screened. Following that, the sample dependency network was constructed based on the Pearson connection coefficients of DEGs in the samples. The prognosis-related genes were collected using logistic regression analysis...
May 9, 2017: Neoplasma
https://www.readbyqxmd.com/read/28385383/prognostic-significance-of-gene-amplification-of-actn4-in-stage-i-and-ii-oral-tongue-cancer
#8
T Kakuya, T Mori, S Yoshimoto, Y Watabe, N Miura, H Shoji, K Onidani, T Shibahara, K Honda
Despite complete resection of the early stage of oral tongue cancer by partial glossectomy, late cervical lymph node metastasis is frequently observed. Gene amplification of ACTN4 (protein name: actinin-4) is closely associated with the metastatic potential of various cancers. This retrospective study was performed to demonstrate the potential usefulness of ACTN4 gene amplification as a prognostic biomarker in patients with stage I/II oral tongue cancer. Fifty-four patients with stage I/II oral tongue cancer were enrolled retrospectively, in accordance with the reporting recommendations for tumour marker prognostic studies (REMARK) guidelines...
April 3, 2017: International Journal of Oral and Maxillofacial Surgery
https://www.readbyqxmd.com/read/28216224/targeted-proteomics-driven-verification-of-biomarker-candidates-associated-with-breast-cancer-aggressiveness
#9
Iva Procházková, Juraj Lenčo, Alena Fučíková, Jiří Dresler, Lenka Čápková, Roman Hrstka, Rudolf Nenutil, Pavel Bouchal
Breast cancer is the most common and molecularly relatively well characterized malignant disease in women, however, its progression to metastatic cancer remains lethal for 78% of patients 5years after diagnosis. Novel markers could identify the high risk patients and their verification using quantitative methods is essential to overcome genetic, inter-tumor and intra-tumor variability and translate novel findings into cancer diagnosis and treatment. We recently identified 13 proteins associated with estrogen receptor, tumor grade and lymph node status, the key factors of breast cancer aggressiveness, using untargeted proteomics...
May 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28111298/aerosol-from-tobacco-heating-system-2-2-has-reduced-impact-on-mouse-heart-gene-expression-compared-with-cigarette-smoke
#10
Justyna Szostak, Stéphanie Boué, Marja Talikka, Emmanuel Guedj, Florian Martin, Blaine Phillips, Nikolai V Ivanov, Manuel C Peitsch, Julia Hoeng
Experimental studies clearly demonstrate a causal effect of cigarette smoking on cardiovascular disease. To reduce the individual risk and population harm caused by smoking, alternative products to cigarettes are being developed. We recently reported on an apolipoprotein E-deficient (Apoe(-/-)) mouse inhalation study that compared the effects of exposure to aerosol from a candidate modified risk tobacco product, Tobacco Heating System 2.2 (THS2.2), and smoke from the reference cigarette (3R4F) on pulmonary and vascular biology...
March 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/27998979/%C3%AE-actinin-4-actn4-regulates-glucocorticoid-receptor-mediated-transactivation-and-transrepression-in-podocytes
#11
Xuan Zhao, Simran Khurana, Sharmistha Charkraborty, Yuqian Tian, John R Sedor, Leslie A Bruggman, Hung-Ying Kao
Glucocorticoids are a general class of steroids that possess renoprotective activity in glomeruli through their interaction with the glucocorticoid receptor. However, the mechanisms by which glucocorticoids ameliorate proteinuria and glomerular disease are not well understood. In this study, we demonstrated that α actinin 4 (ACTN4), an actin-cross-linking protein known to coordinate cytoskeletal organization, interacts with the glucocorticoid receptor (GR) in the nucleus of human podocytes (HPCs), a key cell type in the glomerulus critical for kidney filtration function...
February 3, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27874083/poly-l-ornithine-enhances-migration-of-neural-stem-progenitor-cells-via-promoting-%C3%AE-actinin-4-binding-to-actin-filaments
#12
Hongfei Ge, Anyong Yu, Jingyu Chen, Jichao Yuan, Yi Yin, Wangsheng Duanmu, Liang Tan, Yang Yang, Chuan Lan, Weixiang Chen, Hua Feng, Rong Hu
The recruitment of neural stem/progenitor cells (NSPCs) for brain restoration after injury is a promising regenerative therapeutic strategy. This strategy involves enhancing proliferation, migration and neuronal differentation of NSPCs. To date, the lack of biomaterials, which facilitate these processes to enhance neural regeneration, is an obstacle for the cell replacement therapies. Our previous study has shown that NSPCs grown on poly-L-ornithine (PO) could proliferate more vigorously and differentiate into more neurons than that on Poly-L-Lysine (PLL) and Fibronectin (FN)...
November 22, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27742266/proteomic-analysis-of-mucopolysaccharidosis-i-mouse-brain-with-two-dimensional-polyacrylamide-gel-electrophoresis
#13
Li Ou, Michael J Przybilla, Chester B Whitley
Mucopolysaccharidosis type I (MPS I) is due to deficiency of α-l-iduronidase (IDUA) and subsequent storage of undegraded glycosaminoglycans (GAG). The severe form of the disease, known as Hurler syndrome, is characterized by mental retardation and neurodegeneration of unknown etiology. To identify potential biomarkers and unveil the neuropathology mechanism of MPS I disease, two-dimensional polyacrylamide gel electrophoresis (PAGE) and nanoliquid chromatography-tandem mass spectrometry (nanoLC-MS/MS) were applied to compare proteome profiling of brains from MPS I and control mice (5-month old)...
January 2017: Molecular Genetics and Metabolism
https://www.readbyqxmd.com/read/27613525/a-two-gene-prognostic-classifier-for-early-stage-lung-squamous-cell-carcinoma-in-multiple-large-scale-and-geographically-diverse-cohorts
#14
Rintaro Noro, Teruhide Ishigame, Naomi Walsh, Kouya Shiraishi, Ana I Robles, Bríd M Ryan, Aaron J Schetter, Elise D Bowman, Judith A Welsh, Masahiro Seike, Akihiko Gemma, Vidar Skaug, Steen Mollerup, Aage Haugen, Jun Yokota, Takashi Kohno, Curtis C Harris
INTRODUCTION: There are no validated molecular methods that prospectively identify patients with surgically resected lung squamous cell carcinoma (SCC) at high risk for recurrence. By focusing on the expression of genes with known functions in development of lung SCC and prognosis, we sought to develop a robust prognostic classifier of early-stage lung SCC. METHODS: The expression of 253 genes selected by literature search was evaluated in microarrays from 107 stage I/II tumors...
January 2017: Journal of Thoracic Oncology
https://www.readbyqxmd.com/read/27613243/crucial-genes-associated-with-diabetic-nephropathy-explored-by-microarray-analysis
#15
Zhikui Wang, Zhaoxia Wang, Zhongqi Zhou, Yueqin Ren
BACKGROUND: This study sought to investigate crucial genes correlated with diabetic nephropathy (DN), and their potential functions, which might contribute to a better understanding of DN pathogenesis. METHODS: The microarray dataset GSE1009 was downloaded from Gene Expression Omnibus, including 3 diabetic glomeruli samples and 3 healthy glomeruli samples. The differentially expressed genes (DEGs) were identified by LIMMA package. Their potential functions were then analyzed by the GO and KEGG pathway enrichment analyses using the DAVID database...
September 9, 2016: BMC Nephrology
https://www.readbyqxmd.com/read/27573339/novel-nphs2-variant-in-patients-with-familial-steroid-resistant-nephrotic-syndrome-with-early-onset-slow-progression-and-dominant-inheritance-pattern
#16
Maija Suvanto, Jaakko Patrakka, Timo Jahnukainen, Pia-Maria Sjöström, Matti Nuutinen, Pekka Arikoski, Janne Kataja, Marjo Kestilä, Hannu Jalanko
BACKGROUND: Steroid-resistant nephrotic syndrome (SRNS) is a common cause of end-stage renal disease in children but also occurs as an adult-onset condition. In a subset of SRNS patients, pathogenic variants are found in genes coding for podocyte foot process proteins. The aim of this study was to define the role of pathogenic variants in Finnish patients with familial and sporadic SRNS. METHODS: We analyzed SRNS-related genes NPHS1, NPHS2, NEPH1, ACTN4, TRPC6, INF2, WT1, CD2AP, LAMB2, and PLCE1 for disease-causing variants using direct sequencing of exons and intron/exon boundaries in all members of a family with dominant SRNS with early onset and slow progression to end-stage renal disease...
August 2017: Clinical and Experimental Nephrology
https://www.readbyqxmd.com/read/27546377/robust-identification-of-ptbp1-dependent-splicing-events-by-a-junction-centric-approach-in-xenopus-laevis
#17
Maud Noiret, Agnès Méreau, Gaëlle Angrand, Marion Bervas, Carole Gautier-Courteille, Vincent Legagneux, Stéphane Deschamps, Hubert Lerivray, Justine Viet, Serge Hardy, Luc Paillard, Yann Audic
Regulation of alternative splicing is an important process for cell differentiation and development. Down-regulation of Ptbp1, a regulatory RNA-binding protein, leads to developmental skin defects in Xenopus laevis. To identify Ptbp1-dependent splicing events potentially related to the phenotype, we conducted RNAseq experiments following Ptbp1 depletion. We systematically compared exon-centric and junction-centric approaches to detect differential splicing events. We showed that the junction-centric approach performs far better than the exon-centric approach in Xenopus laevis...
June 15, 2017: Developmental Biology
https://www.readbyqxmd.com/read/27166725/proteomic-identification-of-non-erythrocytic-alpha-spectrin-1-down-regulation-in-the-pre-optic-area-of-neonatally-estradiol-17%C3%AE-treated-female-adult-rats
#18
Vijayakumar Govindaraj, Addicam Jagannadha Rao
It is well established that sexually dimorphic brain regions, which are critical for reproductive physiology and behavior, are organized by steroid hormones during the first 2 weeks after birth in the rodents. In our recent observation, neonatal exposure to estradiol-17β (E2) in the female rat revealed increase in cyclooxygenase 2 (COX-2) level, sexually dimorphic nucleus (SDN)-pre-optic area (POA) size and down-regulation of synaptogenesis related genes in POA in the adult stage. In the present study, using the same animal model, the protein profile of control and neonatally E2-treated POA was compared by 1D-SDS-PAGE, and the protein that shows a change in abundance was identified by LC-MS/MS analysis...
June 1, 2016: Hormone Molecular Biology and Clinical Investigation
https://www.readbyqxmd.com/read/27155012/elucidation-of-tonic-and-activated-b-cell-receptor-signaling-in-burkitt-s-lymphoma-provides-insights-into-regulation-of-cell-survival
#19
Jasmin Corso, Kuan-Ting Pan, Roland Walter, Carmen Doebele, Sebastian Mohr, Hanibal Bohnenberger, Philipp Ströbel, Christof Lenz, Mikolaj Slabicki, Jennifer Hüllein, Federico Comoglio, Michael A Rieger, Thorsten Zenz, Jürgen Wienands, Michael Engelke, Hubert Serve, Henning Urlaub, Thomas Oellerich
Burkitt's lymphoma (BL) is a highly proliferative B-cell neoplasm and is treated with intensive chemotherapy that, because of its toxicity, is often not suitable for the elderly or for patients with endemic BL in developing countries. BL cell survival relies on signals transduced by B-cell antigen receptors (BCRs). However, tonic as well as activated BCR signaling networks and their relevance for targeted therapies in BL remain elusive. We have systematically characterized and compared tonic and activated BCR signaling in BL by quantitative phosphoproteomics to identify novel BCR effectors and potential drug targets...
May 17, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27121206/efficacy-of-adjuvant-chemotherapy-for-non-small-cell-lung-cancer-assessed-by-metastatic-potential-associated-with-actn4
#20
Nami Miura, Masahiro Kamita, Takanori Kakuya, Yutaka Fujiwara, Koji Tsuta, Hideaki Shiraishi, Fumitaka Takeshita, Takahiro Ochiya, Hirokazu Shoji, Wilber Huang, Yuichiro Ohe, Tesshi Yamada, Kazufumi Honda
Although several clinical trials have demonstrated the benefits of platinum-combined adjuvant chemotherapy for resected non-small cell lung cancer (NSCLC), predictive biomarkers for the efficacy of such therapy have not yet been identified. Selection of patients with high metastatic ability in the early stage of non-small cell lung cancer (NSCLC) has the potential to predict clinical benefit of adjuvant chemotherapy (ADJ).In order to develop a predictive biomarker for efficacy of ADJ, we reanalyzed patient data using a public database enrolled by JBR...
May 31, 2016: Oncotarget
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