keyword
MENU ▼
Read by QxMD icon Read
search

Bacteria, antibiotic resistance

keyword
https://www.readbyqxmd.com/read/29222409/immune-stealth-driven-o2-serotype-prevalence-and-potential-for-therapeutic-antibodies-against-multidrug-resistant-klebsiella-pneumoniae
#1
Meghan E Pennini, Anna De Marco, Mark Pelletier, Jessica Bonnell, Romana Cvitkovic, Martina Beltramello, Elisabetta Cameroni, Siro Bianchi, Fabrizia Zatta, Wei Zhao, Xiaodong Xiao, Maria M Camara, Antonio DiGiandomenico, Elena Semenova, Antonio Lanzavecchia, Paul Warrener, JoAnn Suzich, Qun Wang, Davide Corti, C Kendall Stover
Emerging multidrug-resistant bacteria are a challenge for modern medicine, but how these pathogens are so successful is not fully understood. Robust antibacterial vaccines have prevented and reduced resistance suggesting a pivotal role for immunity in deterring antibiotic resistance. Here, we show the increased prevalence of Klebsiella pneumoniae lipopolysaccharide O2 serotype strains in all major drug resistance groups correlating with a paucity of anti-O2 antibodies in human B cell repertoires. We identify human monoclonal antibodies to O-antigens that are highly protective in mouse models of infection, even against heavily encapsulated strains...
December 8, 2017: Nature Communications
https://www.readbyqxmd.com/read/29222103/a-novel-non-antibiotic-lgt-based-selection-system-for-stable-maintenance-of-expression-vectors-in-escherichia-coli-and-vibrio-cholerae
#2
Manuela Terrinoni, Stefan L Nordqvist, Susanne Källgård, Jan Holmgren, Michael Lebens
Antibiotic selection for maintenance of expression plasmids is discouraged in production of recombinant proteins for pharmaceutical or other human uses due to the risks of antibiotic residue contamination of the final products and release of DNA encoding antibiotic resistance into the environment. We describe the construction of expression plasmids that are instead maintained by complementation of the lgt gene encoding a (pro)lipoprotein glyceryl transferase essential for biosynthesis of bacterial lipoprotein...
December 8, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29222019/a-novel-use-of-cellulose-based-filter-paper-containing-silver-nanoparticles-for-its-potential-application-as-wound-dressing-agent
#3
Adnan Haider, Sajjad Haider, Inn-Kyu Kang, Anuj Kumar, Madhusudana Rao Kummara, Tahseen Kamal, Sung Soo Han
The frequent use of antibiotics against microbial infections may lead the emergence of antibiotic resistant microbial strains. To overcome these microbial strains, we need to fabricate alternative materials which can handle them. It is for this reason, we have fabricated cellulose (CE) based filter paper (FP) composite scaffolds comprising of adsorbed chitosan (CS) and sliver (Ag) nanoparticles (NPs). The AgNPs are incorporated in the CS layer of the composite scaffold. Prior to evaluate the efficacy of the scaffolds against gram positive and gram negative bacterial strains, the scaffolds were characterized for the presence of the Ag NPs with field emission scanning electron microscope (FE-SEM), fourier transform infrared (FTIR) spectroscopy and x-ray diffractometer (XRD)...
December 5, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29220785/metagenomic-exploration-reveals-a-marked-change-in-the-river-resistome-and-mobilome-after-treated-wastewater-discharges
#4
Itziar Lekunberri, José Luis Balcázar, Carles M Borrego
Mobile genetic elements (MGEs) are key agents in the spread of antibiotic resistance genes (ARGs) across environments. Here we used metagenomics to compare the river resistome (collection of all ARGs) and mobilome (e.g., integrases, transposases, integron integrases and insertion sequence common region "ISCR" elements) between samples collected upstream (n = 6) and downstream (n = 6) of an urban wastewater treatment plant (UWWTP). In comparison to upstream metagenomes, downstream metagenomes showed a drastic increase in the abundance of ARGs, as well as markers of MGEs, particularly integron integrases and ISCR elements...
December 5, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/29220507/assessing-the-heterogeneity-of-in-silico-plasmid-predictions-based-on-whole-genome-sequenced-clinical-isolates
#5
Cedric C Laczny, Valentina Galata, Achim Plum, Andreas E Posch, Andreas Keller
High-throughput next-generation shotgun sequencing of pathogenic bacteria is growing in clinical relevance, especially for chromosomal DNA-based taxonomic identification and for antibiotic resistance prediction. Genetic exchange is facilitated for extrachromosomal DNA, e.g. plasmid-borne antibiotic resistance genes. Consequently, accurate identification of plasmids from whole-genome sequencing (WGS) data remains one of the major challenges for sequencing-based precision medicine in infectious diseases. Here, we assess the heterogeneity of four state-of-the-art tools (cBar, PlasmidFinder, plasmidSPAdes and Recycler) for the in silico prediction of plasmid-derived sequences from WGS data...
December 5, 2017: Briefings in Bioinformatics
https://www.readbyqxmd.com/read/29219107/molecular-methods-for-detection-of-antimicrobial-resistance
#6
Muna F Anjum, Ea Zankari, Henrik Hasman
The increase in bacteria harboring antimicrobial resistance (AMR) is a global problem because there is a paucity of antibiotics available to treat multidrug-resistant bacterial infections in humans and animals. Detection of AMR present in bacteria that may pose a threat to veterinary and public health is routinely performed using standardized phenotypic methods. Molecular methods are often used in addition to phenotypic methods but are set to replace them in many laboratories due to the greater speed and accuracy they provide in detecting the underlying genetic mechanism(s) for AMR...
December 2017: Microbiology Spectrum
https://www.readbyqxmd.com/read/29219055/the-role-of-nanoparticles-in-the-inhibition-of-multidrug-resistant-bacteria-and-biofilms
#7
Manaf AlMatar, Essam A Makky, Isil Var, Fatih Koksal
BACKGROUND: Until recently, one of the main reasons for mortality has been infectious diseases, and bacteria that are drug-resistant have emerged as a result of the wide application, as well as the misuse, of antibacterial medications. Having multidrug-resistance, bacteria present a great problem for the efficient management of bacterial infections and this challenge has resulted in the creation of other means of dealing with bacterial diseases. Of late, metallic nanoparticles (NPs), employed as antibacterial agents, have the potential for use against resistance to bacterial drugs...
December 7, 2017: Current Drug Delivery
https://www.readbyqxmd.com/read/29218855/effects-of-prior-exposure-to-antibiotics-on-bacterial-adaptation-to-phages
#8
Flor I Arias-Sánchez, Richard C Allen, Alex R Hall
Understanding adaptation to complex environments requires information about how exposure to one selection pressure affects adaptation to others. For bacteria, antibiotics and viral parasites (phages) are two of the most common selection pressures and are both relevant for treatment of bacterial infections: increasing antibiotic resistance is generating significant interest in using phages in addition or as an alternative to antibiotics. However, we lack knowledge of how exposure to antibiotics affects bacterial responses to phages...
December 8, 2017: Journal of Evolutionary Biology
https://www.readbyqxmd.com/read/29218843/potential-of-saprophage-diptera-to-acquire-culturable-livestock-associated-antibiotic-resistant-bacteria
#9
S M Russell, V A Chittick, D T Sangweme, E C Lampert
The increasing prevalence of antibiotic resistance among bacteria is one of the most intractable challenges in 21st-century public health. Dipterans that associate with livestock, livestock waste products and cadavers have the potential to acquire livestock-associated antibiotic-resistant bacteria (LA-ARB) and transmit them to humans. In this study, piglet cadavers were used to attract saprophage dipterans from the environment and those dipterans were sampled for the presence of LA-ARB. In the first trial, culturable microbes resistant to both aminoglycoside and β-lactam antibiotics were found in all cadavers and masses of dipteran larvae, and in three-quarters of adult dipterans...
December 8, 2017: Zoonoses and Public Health
https://www.readbyqxmd.com/read/29218583/promising-antibacterial-effects-of-silver-nanoparticle-loaded-tea-tree-oil-nanoemulsion-a-synergistic-combination-against-resistance-threat
#10
Roqya Najafi-Taher, Behnaz Ghaemi, Sharmin Kharazi, Samira Rasoulikoohi, Amir Amani
Highly resistant pathogens may be developed in patients with immune disorders after prolonged exposure to antibiotics, a growing threat worldwide. In order to overcome these problems, this study introduces a new class of engineered nanosystems comprising of tea tree oil nanoemulsion (TTO NE) loaded with Ag nanoparticles (NPs). Silver shows a strong toxicity towards a wide range of microorganisms. Also, TTO NE could be employed as a promising and safe antimicrobial agent for local therapies of bacterial infections...
December 7, 2017: AAPS PharmSciTech
https://www.readbyqxmd.com/read/29218506/in-silico-discovery-of-novel-ligands-for-antimicrobial-lipopeptides-for-computer-aided-drug-design
#11
REVIEW
Satya Eswari Jujjavarapu, Swasti Dhagat
The increase in antibiotic-resistant strains of pathogens has created havoc worldwide. These antibiotic-resistant pathogens require potent drugs for their inhibition. Lipopeptides, which are produced as secondary metabolites by many microorganisms, have the ability to act as potent safe drugs. Lipopeptides are amphiphilic molecules containing a lipid chain bound to the peptide. They exhibit broad-spectrum activities against both bacteria and fungi. Other than their antimicrobial properties, they have displayed anti-cancer properties as well, but their mechanism of action is not understood...
December 7, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/29218505/antibacterial-and-antibiofilm-activity-of-lactic-acid-bacteria-isolated-from-traditional-artisanal-milk-cheese-from-northeast-china-against-enteropathogenic-bacteria
#12
Xingyang Cui, Yunjia Shi, Shanshan Gu, Xin Yan, Hongyan Chen, Junwei Ge
The present study aims to investigate the probiotic properties of novel strains of lactic acid bacteria isolated from traditional artisanal milk cheese from Northeast China and to explore their antibacterial activity against enteropathogenic bacteria. Of the 321 isolates, 86 exhibited survival in low pH, resistance to pancreatin, and tolerance to bile salts; of these, 12 inhibited the growth of more than seven enteropathogenic bacteria and exhibited antibiofilm activities against Staphylococcus aureus CMCC26003 and/or Escherichia coli CVCC230...
December 7, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/29217934/the-prevalence-of-antibiotic-resistant-diarrhogenic-bacterial-species-in-surface-waters-south-eastern-nigeria
#13
Stanley C Onuoha
Background: This study assessed the bacteriological qualities of surface waters in Afikpo, between April and September 2016. Methods: Surface water samples were collected from three streams for bacteriological analysis. Bacteria species were isolated using standard microbiological and biochemical techniques. Antibiotic susceptibility study was carried out using Kirby Bauer disc diffusion method. Results: The result of the mean heterotrophic bacteria count from the streams showed that Okpu stream had 209...
July 2017: Ethiopian Journal of Health Sciences
https://www.readbyqxmd.com/read/29215335/previous-antibiotic-exposure-and-antimicrobial-resistance-patterns-of-acinetobacter-spp-and-pseudomonas-aeruginosa-isolated-from-patients-with-nosocomial-infections
#14
Zorana M Djordjevic, Marko M Folic, Slobodan M Jankovic
BACKGROUND: The alarming spread of antibiotic-resistant bacteria causing healthcare-associated infections has been extensively reported in recent medical literature. AIMS: To compare trends in antimicrobial consumption and development of resistance among isolates of Acinetobacter spp. and Pseudomonas aeruginosa that cause hospital infections. STUDY DESIGN: Cross-sectional study. METHODS: A study was conducted in a tertiary healthcare institution in central Serbia, during the 7-year period between January 2009 and December 2015...
December 1, 2017: Balkan Medical Journal
https://www.readbyqxmd.com/read/29213101/modulation-of-the-neisseria-gonorrhoeae-drug-efflux-conduit-mtre
#15
Giulia Tamburrino, Salomé Llabrés, Owen N Vickery, Samantha J Pitt, Ulrich Zachariae
Widespread antibiotic resistance, especially of Gram-negative bacteria, has become a severe concern for human health. Tripartite efflux pumps are one of the major contributors to resistance in Gram-negative pathogens, by efficiently expelling a broad spectrum of antibiotics from the organism. In Neisseria gonorrhoeae, one of the first bacteria for which pan-resistance has been reported, the most expressed efflux complex is MtrCDE. Here we present the electrophysiological characterisation of the outer membrane component MtrE and the membrane fusion protein MtrC, obtained by a combination of planar lipid bilayer recordings and in silico techniques...
December 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29211886/bicarbonate-induces-high-level-resistance-to-the-human-antimicrobial-peptide-ll-37-in-staphylococcus-aureus-small-colony-variants
#16
Ping Zhang, John A Wright, Anna Tymon, Sean P Nair
Objectives: Staphylococcus aureus small colony variants (SCVs) cause persistent infections and are resistant to cationic antibiotics. Antimicrobial peptides (AMPs) have been suggested as promising alternatives for treating antibiotic-resistant bacteria. We investigated the capacity of the human cationic AMP LL-37 to kill SCVs in the presence of physiological concentrations of bicarbonate, which are reported to alter bacterial membrane permeability and change resistance of bacteria to AMPs...
December 4, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29211780/prevalence-and-antibiotic-resistance-of-pseudomonas-aeruginosa-in-water-samples-in-central-italy-and-molecular-characterization-of-oprd-in-imipenem-resistant-isolates
#17
Giuditta Fiorella Schiavano, Elisa Carloni, Francesca Andreoni, Silvia Magi, Maria Chironna, Giorgio Brandi, Giulia Amagliani
SCOPE: This study aimed to analyse the prevalence, antibiotic resistance and genetic relatedness of P. aeruginosa isolates obtained from potable and recreational water samples (n. 8,351) collected from different settings (swimming pools, n. 207; healthcare facilities, n 1,684; accommodation facilities, n. 1,518; municipal waterworks, n. 4,500; residential buildings, n. 235). Possible mechanisms underlying resistance to imipenem, with particular focus on those involving oprD-based uptake, were also explored...
2017: PloS One
https://www.readbyqxmd.com/read/29211019/dual-regulation-of-the-small-rna-micc-and-the-quiescent-porin-ompn-in-response-to-antibiotic-stress-in-escherichia-coli
#18
Sushovan Dam, Jean-Marie Pagès, Muriel Masi
Antibiotic resistant Gram-negative bacteria are a serious threat for public health. The permeation of antibiotics through their outer membrane is largely dependent on porin, changes in which cause reduced drug uptake and efficacy. Escherichia coli produces two major porins, OmpF and OmpC. MicF and MicC are small non-coding RNAs (sRNAs) that modulate the expression of OmpF and OmpC, respectively. In this work, we investigated factors that lead to increased production of MicC. micC promoter region was fused to lacZ, and the reporter plasmid was transformed into E...
December 6, 2017: Antibiotics
https://www.readbyqxmd.com/read/29209794/advances-in-therapeutic-bacterial-antisense-biotechnology
#19
REVIEW
John P Hegarty, David B Stewart
Antisense therapeutics are a biotechnological form of antibiotic therapy using chemical analogues of short single-stranded nucleic acid sequences modified to form stable oligomers. These molecules are termed antisense oligonucleotides (ASOs) because their sequence is complementary, via Watson-Crick specific base pairing, to their target messenger RNA (mRNA). ASOs modify gene expression in this sequence-dependent manner by binding to its complementary mRNA and inhibiting its translation into protein through steric blockage and/or through RNase degradation of the ASO/RNA duplex...
December 5, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29209784/acaxl2-and-acmst1-regulate-arthrospore-development-and-stress-resistance-in-the-cephalosporin-c-producer-acremonium-chrysogenum
#20
Janina Kluge, Ulrich Kück
The filamentous fungus Acremonium chrysogenum is the primordial producer of the β-lactam antibiotic cephalosporin C. This antibiotic is of major biotechnological and medical relevance because of its antibacterial activity against Gram-positive and Gram-negative bacteria. Antibiotic production during the lag phase of fermentation is often accompanied by a typical morphological feature of A. chrysogenum, the fragmentation of the mycelium into arthrospores. Here, we sought to identify factors that regulate the hyphal septation process and present the first comparative functional characterization of the type I integral plasma membrane protein Axl2 (axial budding pattern protein 2), a central component of the bud site selection system (BSSS) and Mst1 (mammalian Sterile20-like kinase), a septation initiation network (SIN)-associated germinal center kinase (GCK)...
December 5, 2017: Current Genetics
keyword
keyword
106942
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"