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treatment for escherichia coli enterotoxigenic

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https://www.readbyqxmd.com/read/28199144/attachment-of-13-types-of-foodborne-bacteria-to-jalape%C3%A3-o-and-serrano-peppers-and-antibacterial-effect-of-roselle-calyx-extracts-sodium-hypochlorite-colloidal-silver-and-acetic-acid-against-these-foodborne-bacteria-on-peppers
#1
Esmeralda Rangel-Vargas, Carlos A Gómez-Aldapa, Reyna N Falfan-Cortes, María L Rodríguez-Marín, Angélica Godínez-Oviedo, Otilio A Acevedo-Sandoval, Javier Castro-Rosas
Chili peppers are a very important crop in Mexico. However, these peppers have been associated with Salmonella infection outbreaks in the United States, and Salmonella and diarrheagenic Escherichia coli pathotypes have been isolated from jalapeño and serrano peppers in Mexico. To decrease microbial contamination of fruits and vegetables, chemical agents are commonly used; however, chemical agents used to eliminate pathogenic bacteria on vegetables have a limited antimicrobial effect. Roselle ( Hibiscus sabdariffa ) calyces have been reported to have an antimicrobial effect on pathogenic bacteria...
February 15, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28056796/the-fecal-presence-of-enterotoxin-and-f4-genes-as-an-indicator-of-efficacy-of-treatment-with-colistin-sulfate-in-pigs
#2
Mohamed Rhouma, John Morris Fairbrother, William Thériault, Francis Beaudry, Nadia Bergeron, Sylvette Laurent-Lewandowski, Ann Letellier
BACKGROUND: Enterotoxigenic Escherichia coli (ETEC) strains producing multiple enterotoxins are important causes of post-weaning diarrhea (PWD) in pigs. The aim of the present study was to investigate the fecal presence of ETEC enterotoxin as well as F4 and F18 genes as an indicator of colistin sulfate (CS) efficacy for treatment of PWD in pigs. Forty-eight piglets were weaned at the age of 21 days, and were divided into four groups: challenged treated, challenged untreated, unchallenged treated, and unchallenged untreated...
January 5, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28034476/defining-cases-of-severe-pediatric-diarrhea-for-an-efficacy-trial-of-an-enterotoxigenic-escherichia-coli-etec-vaccine-report-on-an-international-workshop-washington-dc-march-2016
#3
Thomas F Wierzba, Alexandra Bourgis
Enterotoxigenic Escherichia coli (ETEC) causes severe acute watery diarrhea. No ETEC vaccine is available but candidates are in development, including ETVAX, an oral, whole-cell inactivated vaccine. ETVAX is being tested in a descending-age trial in Bangladesh. If found safe and immunogenic, investigators may test it for efficacy in children. Like oral rotavirus vaccines, we expect that ETVAX will be most effective at decreasing the incidence of moderate-to-severe ETEC episodes. Thus, for an efficacy trial outcome, it will be necessary to triage patients into moderate-to-severe versus mild disease...
January 23, 2017: Vaccine
https://www.readbyqxmd.com/read/27832811/farm-characteristics-and-management-routines-related-to-neonatal-porcine-diarrhoea-a-survey-among-swedish-piglet-producers
#4
Jenny Larsson, Nils Fall, Maria Lindberg, Magdalena Jacobson
BACKGROUND: In recent years reports from a number of countries, including Sweden, describe problems with diarrhoea in newborn piglets despite the use of previously effective preventive measures. This seemingly altered disease pattern of neonatal porcine diarrhoea (NPD) warrants investigations on the magnitude and manifestation of the problem. The aim of the present study was to investigate the herd-level prevalence of NPD in Sweden and to describe disease characteristics and intervention strategies used in affected herds...
November 10, 2016: Acta Veterinaria Scandinavica
https://www.readbyqxmd.com/read/27816246/clinical-trial-on-the-effects-of-a-free-access-acidified-milk-replacer-feeding-program-on-the-health-and-growth-of-dairy-replacement-heifers-and-veal-calves
#5
C G Todd, K E Leslie, S T Millman, V Bielmann, N G Anderson, J M Sargeant, T J DeVries
The objectives of this study were to evaluate the effects of free-access acidified milk replacer feeding on the pre- and postweaning health of dairy and veal calves. Individually housed calves were systematically assigned at birth to 1 of 2 feeding programs: free-access feeding (ad libitum) of acidified milk replacer (ACD, n=249) or traditional restricted feeding (3L fed twice daily) of milk replacer (RES, n=249). Calves were fed milk replacer containing 24% crude protein and 18% fat. Acidified milk replacer was prepared to a target pH between 4...
January 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/27688074/differential-protection-by-cell-wall-components-of-lactobacillus-amylovorus-dsm-16698-t-against-alterations-of-membrane-barrier-and-nf-kb-activation-induced-by-enterotoxigenic-f4-escherichia-coli-on-intestinal-cells
#6
Marianna Roselli, Alberto Finamore, Ulla Hynönen, Airi Palva, Elena Mengheri
BACKGROUND: The role of Lactobacillus cell wall components in the protection against pathogen infection in the gut is still largely unexplored. We have previously shown that L. amylovorus DSM 16698(T) is able to reduce the enterotoxigenic F4(+) Escherichia coli (ETEC) adhesion and prevent the pathogen-induced membrane barrier disruption through the regulation of IL-10 and IL-8 expression in intestinal cells. We have also demonstrated that L. amylovorus DSM 16698(T) protects host cells through the inhibition of NF-kB signaling...
September 29, 2016: BMC Microbiology
https://www.readbyqxmd.com/read/27607555/gut-microbiota-inflammation-and-colorectal-cancer
#7
Caitlin A Brennan, Wendy S Garrett
Colorectal cancer is the second-leading cause of cancer-related deaths in the United States and fourth-leading cause of cancer-related deaths worldwide. While cancer is largely considered to be a disease of genetic and environmental factors, increasing evidence has demonstrated a role for the microbiota (the microorganisms associated with the human body) in shaping inflammatory environments and promoting tumor growth and spread. Herein, we discuss both human data from meta'omics analyses and data from mechanistic studies in cell culture and animal models that support specific bacterial agents as potentiators of tumorigenesis-including Fusobacterium nucleatum, enterotoxigenic Bacteroides fragilis, and colibactin-producing Escherichia coli...
September 8, 2016: Annual Review of Microbiology
https://www.readbyqxmd.com/read/27574018/administration-of-non-pathogenic-isolates-of-escherichia-coli-and-clostridium-perfringens-type-a-to-piglets-in-a-herd-affected-with-a-high-incidence-of-neonatal-diarrhoea
#8
C Unterweger, A Kahler, G-F Gerlach, M Viehmann, A von Altrock, I Hennig-Pauka
A bacterial cocktail of living strains of Clostridium perfringens type A (CPA) without β2-toxin gene and non-pathogenic Escherichia coli was administered orally to newborn piglets before first colostrum intake and on 2 consecutive days on a farm with a high incidence of diarrhoea and antibiotic treatment in suckling piglets associated with E. coli and CPA. This clinical field study was driven by the hypothetic principle of competitive exclusion of pathogenic bacteria due to prior colonization of the gut mucosal surface by non-pathogenic strains of the same bacterial species with the aim of preventing disease...
August 30, 2016: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/27428376/resistance-pattern-and-molecular-characterization-of-enterotoxigenic-escherichia-coli-etec-strains-isolated-in-bangladesh
#9
Yasmin A Begum, K A Talukder, Ishrat J Azmi, Mohammad Shahnaij, A Sheikh, Salma Sharmin, A-M Svennerholm, Firdausi Qadri
BACKGROUND: Enterotoxigenic Escherichia coli (ETEC) is a common cause of bacterial infection leading to acute watery diarrhea in infants and young children as well as in travellers to ETEC endemic countries. Ciprofloxacin is a broad-spectrum antimicrobial agent nowadays used for the treatment of diarrhea. This study aimed to characterize ciprofloxacin resistant ETEC strains isolated from diarrheal patients in Bangladesh. METHODS: A total of 8580 stool specimens from diarrheal patients attending the icddr,b Dhaka hospital was screened for ETEC between 2005 and 2009...
2016: PloS One
https://www.readbyqxmd.com/read/27234971/in-vivo-therapeutic-efficacy-and-pharmacokinetics-of-colistin-sulfate-in-an-experimental-model-of-enterotoxigenic-escherichia-coli-infection-in-weaned-pigs
#10
Mohamed Rhouma, Francis Beaudry, William Thériault, Nadia Bergeron, Guy Beauchamp, Sylvette Laurent-Lewandowski, John Morris Fairbrother, Ann Letellier
Enterotoxigenic Escherichia coli (ETEC: F4) associated with post-weaning diarrhea (PWD) in pigs has developed resistance against several antimicrobial families, leading to increased use of colistin sulfate (CS) for the treatment of this disease. The objective of this study was to determine the efficacy of oral CS treatment in experimental PWD due to ETEC: F4 challenge and determine the effect of this challenge on CS intestinal absorption. In this study, 96 pigs were divided into two trials based on CS dose (100 000 or 50 000 IU/kg)...
2016: Veterinary Research
https://www.readbyqxmd.com/read/27234415/-traveller-s-diarrhoea
#11
Jordi Vila, Ines Oliveira, Yuliya Zboromyrska, Joaquim Gascon
Traveller's diarrhoea (TD) is acquired primarily through ingestion of food and drinks contaminated with pathogens that cause diarrhoea. They can be bacteria, protozoa, helminths, and viruses. Globally, the most common causes of TD are two pathotypes of Escherichia coli (enterotoxigenic and enteroaggregative) and Campylobacter, although there are significant variations by geographic area visited. Most TD occurs in individuals traveling to low-middle income countries. The type of travel, length of stay, traveller's age, and the presence of certain underlying conditions are important risk factors to consider for the acquisition of TD...
November 2016: Enfermedades Infecciosas y Microbiología Clínica
https://www.readbyqxmd.com/read/27166825/igy-against-enterotoxigenic-escherichia-coli-administered-by-hydrogel-carbon-nanotubes-composites-to-prevent-neonatal-diarrhoea-in-experimentally-challenged-piglets
#12
Fabrisio Alustiza, Romina Bellingeri, Natalia Picco, Carlos Motta, Maria C Grosso, Cesar A Barbero, Diego F Acevedo, Adriana Vivas
In previous studies, the applicability of polymeric hydrogels for the protection of egg yolk immunoglobulin (IgY) against simulated gastric conditions was established. Thereafter, the performance of the hydrogels was improved with the addition of chitosan wrapped carbon nanotubes and the in vitro toxicity for porcine intestinal cells of these nanocomposites was assessed. The objective of the present study was to evaluate in vivo the protective efficacy of the nanocomoposite matrix for IgY when the immunoglobulin is used against enterotoxigenic Escherichia coli (ETEC) in challenged piglets...
June 14, 2016: Vaccine
https://www.readbyqxmd.com/read/27032504/protective-effects-of-lactobacillus-plantarum-on-epithelial-barrier-disruption-caused-by-enterotoxigenic-escherichia-coli-in-intestinal-porcine-epithelial-cells
#13
Yunpeng Wu, Cui Zhu, Zhuang Chen, Zhongjian Chen, Weina Zhang, Xianyong Ma, Li Wang, Xuefen Yang, Zongyong Jiang
Tight junctions (TJs) play an important role in maintaining the mucosal barrier function and gastrointestinal health of animals. Lactobacillus plantarum (L. plantarum) was reported to protect the intestinal barrier function of early-weaned piglets against enterotoxigenic Escherichia coli (ETEC) K88 challenge; however, the underlying cellular mechanism of this protection was unclear. Here, an established intestinal porcine epithelia cell (IPEC-J2) model was used to investigate the protective effects and related mechanisms of L...
April 2016: Veterinary Immunology and Immunopathology
https://www.readbyqxmd.com/read/26960316/discovery-of-the-cell-penetrating-function-of-a2-domain-derived-from-lta-subunit-of-escherichia-coli-heat-labile-enterotoxin
#14
Di Liu, Hua Guo, Wenyun Zheng, Na Zhang, Tianwen Wang, Ping Wang, Xingyuan Ma
Heat-labile enterotoxin (LT) is a protein toxin produced by enterotoxigenic Escherichia coli (ETEC). As a bacterial toxin, LT holotoxin can enter intestinal epithelial cells and cause diarrhea. In addition, LT is also a powerful mucosal adjuvant capable of enhancing the strong immune responses to co-administered antigens. However, the LT immunological mechanism is still not clear in some aspects, especially with the respect to how the LTA subunit functions alone. Here, we discovered that the A2 domain of LTA could carry a fluorescent protein into cells, whose function is similar to a cell-penetrating peptide...
June 2016: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/26819705/effects-of-dietary-supplementation-of-probiotic-clostridium-butyricum-on-growth-performance-immune-response-intestinal-barrier-function-and-digestive-enzyme-activity-in-broiler-chickens-challenged-with-escherichia-coli-k88
#15
Ling Zhang, Lingling Zhang, Xiu'an Zhan, Xinfu Zeng, Lin Zhou, Guangtian Cao, An'guo Chen, Caimei Yang
BACKGROUND: Colibacillosis caused by enterotoxigenic Escherichia coli (E. coli) results in economic losses in the poultry industry. Antibiotics are usually used to control colibacillosis, however, E. coli has varying degrees of resistance to different antibiotics. Therefore the use of probiotics is becoming accepted as an alternative to antibiotics. In this study, we evaluated the effects of Clostridium butyricum (C. butyricum) on growth performance, immune response, intestinal barrier function, and digestive enzyme activity in broiler chickens challenged with Escherichia coli (E...
2016: Journal of Animal Science and Biotechnology
https://www.readbyqxmd.com/read/26758686/identification-and-antimicrobial-resistance-prevalence-of-pathogenic-escherichia-coli-strains-from-treated-wastewater-effluents-in-eastern-cape-south-africa
#16
Martins A Adefisoye, Anthony I Okoh
Antimicrobial resistance (AMR) is a global problem impeding the effective prevention/treatment of an ever-growing array of infections caused by pathogens; a huge challenge threatening the achievements of modern medicine. In this paper, we report the occurrence of multidrug resistance (MDR) in Escherichia coli strains isolated from discharged final effluents of two wastewater treatment facilities in the Eastern Cape Province of South Africa. Standard disk diffusion method was employed to determine the antibiotic susceptibility profile of 223 polymerase chain reaction (PCR)-confirmed E...
February 2016: MicrobiologyOpen
https://www.readbyqxmd.com/read/26635958/response-to-an-escherichia-coli-k88-oral-challenge-and-productivity-of-weanling-pigs-receiving-a-dietary-nucleotides-supplement
#17
Hanlin Li, Pinyao Zhao, Yan Lei, Tianshui Li, InHo Kim
BACKGROUND: Dietary nucleotides, considered as antibiotics alternative, were shown to have positive effects on intestinal hyperaemia, systemic immunity, small-intestinal growth, and hepatic composition in pigs. However, there is no previous research on nucleotide supplementation in weanling pigs under an oral challenged E. coli K88. Therefore, 2 experiments were conducted to investigate the effects of dietary nucleotides on weanling pig growth performance, nutrient digestibility, fecal score, and blood profile after being orally challenged with E...
2015: Journal of Animal Science and Biotechnology
https://www.readbyqxmd.com/read/26497319/designing-and-structure-evaluation-of-multi-epitope-vaccine-against-etec-and-ehec-an-in-silico-approach
#18
Fatemeh S Jeshvaghani, Ahmad K Rahjerdi, Jafar Amani, Iman Rad, Mahyat Jafari, Ali H Salmanian
Diarrheal diseases represent a major health problem in developing countries. Several viruses and bacterial agents, such as Enterotoxigenic Escherichia coli (ETEC) and Enterohemorrhagic Escherichia coli (EHEC) are responsible for human enteric infections. In humans, EHEC infections result in bloody or non-bloody diarrhea, which may be complicated by haemorrhagic colitis and haemolytic uraemic syndrome (HUS). Infection by ETEC is accompanied by a non inflammatory watery diarrhea. E. coli follows a common strategy of infection: colonization on a mucosal site, evasion of host defenses, multiplication, and host damage...
2016: Protein and Peptide Letters
https://www.readbyqxmd.com/read/26424597/structural-signature-of-ser83leu-and-asp87asn-mutations-in-dna-gyrase-from-enterotoxigenic-escherichia-coli-and-impact-on-quinolone-resistance
#19
Kusum Mehla, Jayashree Ramana
Enterotoxigenic Escherichia coli (ETEC) is among the most frequent microorganisms causing traveler's diarrhea (TD). Quinolones are potent antimicrobial agents used for the treatment of TD. Resistance to quinolones is typically caused by substitutions in QRDR region of gyrA subunit of DNA gyrase. The aim of this study was to seek insights into the effect of these substitutions at structural level and their association with observed quinolone resistance. Majority of the ETEC strains have gyrA mutations at amino acid position 83 and 87...
January 15, 2016: Gene
https://www.readbyqxmd.com/read/26323374/-efficacy-of-disinfection-treatments-using-essential-oils-and-ultrasound-on-tomato-fruits-inoculated-with-escherichia-coli-and-impact-on-antioxidant-activity
#20
COMPARATIVE STUDY
María L Luna Guevara, Juan J Luna Guevara, Héctor Ruiz Espinosa, Lucero Leyva Abascal, Carolina B Díaz González
Fresh produce often harbors a great number of microorganisms; hence, its growing demand may constitute a risk for consumers. The aim of this study was to evaluate the efficacy of several disinfection procedures against enterotoxigenic Escherichia coli (ETEC) inoculated on tomato fruits and the conservation of the antioxidant properties of these disinfected fruits. Fruits were immersed for 5 or 10min in oregano or thyme essential oil dispersions (5, 10ppm), with or without ultrasound treatment. Antioxidant activity of disinfected fruits was determined as the ability to scavenge 2,2-diphenyl-1-pricrylhydrazyl (DPPH) radicals and was reported as percentage of inhibition (%I)...
July 2015: Revista Argentina de Microbiología
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