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https://www.readbyqxmd.com/read/28646383/anterior-cruciate-ligament-graft-fixation-first-in-anterior-and-posterior-cruciate-ligament-reconstruction-best-restores-knee-kinematics
#1
Libin Zheng, Soheil Sabzevari, Brandon Marshall, Junjun Zhu, Monica A Linde, Patrick Smolinski, Freddie H Fu
PURPOSE: To evaluate the effect of different graft fixation sequences in one-stage anterior cruciate ligament (ACL) and posterior cruciate ligament (PCL) reconstruction on (1) knee biomechanics and (2) tibiofemoral alignment. METHODS: Twelve porcine knees were used in this study. Five fixation sequences were performed (angle indicating knee flexion): (a) PCL at 30° and ACL at 30°, (b) PCL at 90° and ACL at 30°, (c) ACL at 30° and PCL at 30°, (d) ACL at 30° and PCL at 90°, and (e) ACL and PCL simultaneous fixation at 30°...
June 23, 2017: Knee Surgery, Sports Traumatology, Arthroscopy: Official Journal of the ESSKA
https://www.readbyqxmd.com/read/28645796/preclinical-testing-of-frequency-tunable-capacitive-micromachined-ultrasonic-transducer-probe-prototypes
#2
Martin Pekař, Alexander F Kolen, Harm Belt, Frank van Heesch, Nenad Mihajlović, Imo E Hoefer, Tamas Szili-Török, Hendrik J Vos, Johan G Bosch, Gijs van Soest, Antonius F W van der Steen
In intracardiac echocardiography (ICE) it may be beneficial to generate ultrasound images acquired at multiple frequencies, having the possibility of high penetration or high-resolution imaging in a single device. The objective of the presented work is to test two frequency-tunable probe prototypes in a preclinical setting: a rigid probe having a diameter of 11 mm and a new flexible and steerable 12-Fr ICE catheter. Both probes feature a forward-looking 32-element capacitive micromachined ultrasonic transducer array (aperture of 2 × 2 mm(2)) operated in collapse mode, which allows for frequency tuning in the 6-MHz-18-MHz range...
June 20, 2017: Ultrasound in Medicine & Biology
https://www.readbyqxmd.com/read/28645676/a-journey-through-growth-plates-tracking-differences-in-morphology-and-regulation-between-the-spine-and-long-bones-in-a-pig-model
#3
Adalbert Raimann, Alireza Javanmardi, Monika Egerbacher, Gabriele Haeusler
BACKGROUND CONTEXT: The process of linear growth is driven by axial elongation of both long bones and vertebral bodies and accomplished by enchondral ossification. Differences in regulation between the two skeletal sites are mirrored clinically by the age course in body proportions. Whereas growth plates in long bones can easily be discriminated, vertebral growth plates are part of the cartilaginous endplate, which typically shows important species differences. PURPOSE: To describe and compare histological, histomorphometric and regulatory characteristics in the growth plates of the spine and long bones in a porcine model...
June 20, 2017: Spine Journal: Official Journal of the North American Spine Society
https://www.readbyqxmd.com/read/28644752/expression-and-purification-of-swine-rag2-in-e-coli-for-production-of-porcine-rag2-polyclonal-antibodies
#4
Yu-Bei Jin, Wen-Tao Yang, Ke-Yan Huang, Hong-Liang Chen, Seria-Masole Shonyela, Jing Liu, Qiong Liu, Bo Feng, You Zhou, Shu-Li Zhi, Yan-Long Jiang, Jian-Zhong Wang, Hai-Bin Huang, Chun-Wei Shi, Gui-Lian Yang, Chun-Feng Wang
Recombination activating gene 2 (RAG2) is necessary for immature B cell differentiation. Antibodies to human and rabbit RAG2 are currently commercially available, but antibodies to swine RAG remain unavailable to date. In this study, the swine RAG2 genes sequence was synthesized and then cloned into a pET-28a vector. The recombinant fusion protein was successfully expressed in E. coli, purified through nickel column chromatography, and further digested with Tobacco Etch Virus protease. The cleaved protein was purified by molecular-exclusion chromatography and named pRAG2...
June 23, 2017: Bioscience, Biotechnology, and Biochemistry
https://www.readbyqxmd.com/read/28644516/hemostatic-efficiency-of-modern-topical-sealants-comparative-evaluation-after-liver-resection-and-splenic-laceration-in-a-swine-model
#5
Hamidreza Fonouni, Arash Kashfi, Ali Majlesara, Oliver Stahlheber, Lukas Konstantinidis, Negin Gharabaghi, Thomas W Kraus, Arianeb Mehrabi, Hani Oweira
Parenchymal transection during hepatobiliary surgery can disrupt small vasculature or bile ducts, which could be managed difficultly. Sealants are helpful tools to achieve better hemostasis. The aim of this study is to analyze the hemostatic efficiency of four modern sealants in a porcine model. In this study, 40 landrace pigs were assigned equally to the control (without sealant) and four sealant groups. Standardized liver resection and splenic lesions were performed and left without using sealant (control) or treated with one of the following sealants: TachoSil(®) , Tissucol Duo(®) , Coseal(®) , and FloSeal(®) ...
June 23, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28644151/ecg-based-4d-dose-reconstruction-of-cardiac-arrhythmia-ablation-with-carbon-ion-beams-application-in-a-porcine-model
#6
Daniel Richter, H Immo Lehmann, Anna Eichhorn, Anna M Constantinescu, Robert Kaderka, Matthias Prall, Patrick Lugenbiel, Mitsuru Takami, Dierk Thomas, Christoph Bert, Marco Durante, Douglas L Packer, Christian Graeff
Noninvasive ablation of cardiac arrhythmia by scanned particle radiotherapy is highly promising, but especially challenging due to cardiac and respiratory motion. Irradiations for catheter-free ablation in intact pigs were carried out at the GSI Helmholtz Center in Darmstadt using scanned carbon ions. Here, we present real-time electrocardiogram (ECG) data to estimate time-resolved (4D) delivered dose. For 11 animals, surface ECGs and temporal structure of beam delivery were acquired during irradiation. R waves were automatically detected from surface ECGs...
June 23, 2017: Physics in Medicine and Biology
https://www.readbyqxmd.com/read/28643555/unexpected-detection-of-porcine-rotavirus-c-strains-carrying-human-origin-vp6-gene
#7
Jobin Jose Kattoor, Sharad Saurabh, Yashpal Singh Malik, Shubhankar Sircar, Kuldeep Dhama, Souvik Ghosh, Krisztián Bányai, Nobumichi Kobayashi, Raj Kumar Singh
BACKGROUND: Rotavirus C (RVC), a known etiological agent of diarrheal outbreaks, mainly inflicts swine population globally with sporadic incidence in human, cattle, ferret, mink and dog. OBJECTIVE: To demonstrate presence of RVC in Indian swine population and characterisation of its selected structural (VP6) and non-structural (NSP4 and NSP5) genes. METHODS: A total of 108 diarrheic samples from different regions of India were used. Isolated RNA was loaded onto polyacrylamide gel to screen for the presence of RVs through the identification of specific electrophoretic genomic migration pattern...
June 23, 2017: Veterinary Quarterly
https://www.readbyqxmd.com/read/28643180/first-complete-genome-sequences-of-genogroup-v-genotype-3-porcine-sapoviruses-common-5-terminal-genomic-feature-of-sapoviruses
#8
Tomoichiro Oka, Yen Hai Doan, Takashi Shimoike, Kei Haga, Takenori Takizawa
Sapoviruses (SaVs) are enteric viruses and have been detected in various mammals. They are divided into multiple genogroups and genotypes based on the entire major capsid protein (VP1) encoding region sequences. In this study, we determined the first complete genome sequences of two genogroup V, genotype 3 (GV.3) SaV strains detected from swine fecal samples, in combination with Illumina MiSeq sequencing of the libraries prepared from viral RNA and PCR products. The lengths of the viral genome (7494 nucleotides [nt] excluding polyA tail) and short 5'-untranslated region (14 nt) as well as two predicted open reading frames are similar to those of other SaVs...
June 22, 2017: Virus Genes
https://www.readbyqxmd.com/read/28642841/strain-and-dose-dependent-reduction-of-toxoplasma-gondii-burden-in-pigs-is-associated-with-interferon-gamma-production-by-cd8-lymphocytes-in-a-heterologous-challenge-model
#9
Malgorzata Jennes, Stéphane De Craeye, Bert Devriendt, Katelijne Dierick, Pierre Dorny, Eric Cox
Toxoplasma gondii is a worldwide prevalent parasite of humans and animals. The global infection burden exceeds yearly one million disability-adjusted life years (DALY's) in infected individuals. Therefore, effective preventive measures should be taken to decrease the risk of infection in humans. Although human toxoplasmosis is predominantly foodborne by ingestion of tissue cysts in meat from domestic animals such as pigs, the incidence risk is difficult to estimate due to the lack of screening of animals for infection and insights in location and persistence of the parasite in the tissues...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28641140/effect-of-c1-inh-on-ischemia-reperfusion-injury-in-a-porcine-limb-ex-vivo-perfusion-model
#10
Mai M Abdelhafez, Jane Shaw, Damian Sutter, Jonas Schnider, Yara Banz, Hansjörg Jenni, Esther Voegelin, Mihai A Constantinescu, Robert Rieben
Revascularization of an amputated limb within 4-6h is essential to avoid extensive ischemia/reperfusion (I/R) injury leading to vascular leakage, edema and tissue necrosis. I/R injury is a pathological inflammatory condition that occurs during reperfusion of an organ or tissue after prolonged ischemia. It is characterized by a complex crosstalk between endothelial cell activation and the activation of plasma cascades. Vasculoprotective pharmacological intervention to prevent I/R injury might be an option to prolong the time window between limb amputation and successful replantation...
June 19, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/28640859/toxic-effects-of-atrazine-on-porcine-oocytes-and-possible-mechanisms-of-action
#11
Bao Yuan, Shuang Liang, Yong-Xun Jin, Ming-Jun Zhang, Jia-Bao Zhang, Nam-Hyung Kim
Because atrazine is a widely used herbicide, its adverse effects on the reproductive system have been extensively researched. In this study, we investigated the effects of atrazine exposure on porcine oocyte maturation and the possible mechanisms. Our results showed that the rates of oocyte maturation significantly decreased after treatment with 200 μM atrazine in vitro. Atrazine treatment resulted in abnormal spindle morphology but did not affect actin distribution. Atrazine exposure not only triggered a DNA damage response but also decreased MPF levels in porcine oocytes...
2017: PloS One
https://www.readbyqxmd.com/read/28640820/whole-genome-analysis-of-selected-human-and-animal-rotaviruses-identified-in-uganda-from-2012-to-2014-reveals-complex-genome-reassortment-events-between-human-bovine-caprine-and-porcine-strains
#12
Josephine Bwogi, Khuzwayo C Jere, Charles Karamagi, Denis K Byarugaba, Prossy Namuwulya, Frederick N Baliraine, Ulrich Desselberger, Miren Iturriza-Gomara
Rotaviruses of species A (RVA) are a common cause of diarrhoea in children and the young of various other mammals and birds worldwide. To investigate possible interspecies transmission of RVAs, whole genomes of 18 human and 6 domestic animal RVA strains identified in Uganda between 2012 and 2014 were sequenced using the Illumina HiSeq platform. The backbone of the human RVA strains had either a Wa- or a DS-1-like genetic constellation. One human strain was a Wa-like mono-reassortant containing a DS-1-like VP2 gene of possible animal origin...
2017: PloS One
https://www.readbyqxmd.com/read/28639977/topical-delivery-of-immunosuppression-to-prolong-xenogeneic-and-allogeneic-split-thickness-skin-graft-survival
#13
Melissa Mastroianni, Zhi Yang Ng, Ritu Goyal, Christopher Mallard, Evan A Farkash, David A Leonard, Alexander Albritton, Kumaran Shanmugarajah, Josef M Kurtz, David H Sachs, Lauren K Macri, Joachim Kohn, Curtis L Cetrulo
Cadaveric skin allograft is the current standard of treatment for temporary coverage of large burn wounds. Porcine xenografts are viable alternatives but undergo α-1,3-galactose (Gal)-mediated hyperacute rejection and are lost by POD 3 because of naturally occurring antibodies to Gal in primate recipients. Using baboons, we previously demonstrated that xenografts from GalT-KO swine (lacking Gal) provided wound coverage comparable with allografts with systemic immunosuppression. In this study, we investigate topical immunosuppression as an alternative to prolong xenograft survival...
June 7, 2017: Journal of Burn Care & Research: Official Publication of the American Burn Association
https://www.readbyqxmd.com/read/28639746/acute-changes-in-histopathology-and-intravascular-imaging-after-catheter-based-renal-denervation-in-a-porcine-model
#14
Atsushi Sakaoka, Akiyuki Takami, Yuji Onimura, Hitomi Hagiwara, Hisako Terao, Fumiaki Kumagai, Kiyoshi Matsumura
OBJECTIVES: We first aimed to identify the histopathological changes occurring immediately after renal denervation (RDN) with radiofrequency energy, and then to assess the feasibility of determining procedural success using currently available clinical intravascular imaging techniques. BACKGROUND: Catheter-based RDN has been used as an alternative therapy for hypertension. However, no practical endpoint to determine procedural success during treatment has been established...
June 22, 2017: Catheterization and Cardiovascular Interventions
https://www.readbyqxmd.com/read/28639139/ex-vivo-permeation-of-erythropoietin-through-porcine-conjunctiva-cornea-and-sclera
#15
Ana Paula Resende, Beatriz Silva, Berta São Braz, Telmo Nunes, Lídia Gonçalves, Esmeralda Delgado
The aim of this study is to test the permeation of human recombinant erythropoietin (rHuEPO) across conjunctiva, cornea, and sclera in an ex vivo model. Thirty fresh pig eyes were collected from a slaughterhouse. Conjunctivas (n = 10), corneas (n = 10), and scleras (n = 10) were surgically dissected from surrounding tissues. Ocular membranes were placed into Franz diffusion cells and rHuEPO was administered into the donor phase of each cell, except for control samples. Samples were collected from the receptor phase at seven time points, from 30 min to 6 h of incubation...
June 21, 2017: Drug Delivery and Translational Research
https://www.readbyqxmd.com/read/28639098/a-head-to-head-comparison-of-xinsorb-bioresorbable-sirolimus-eluting-scaffold-versus-metallic-sirolimus-eluting-stent-180-days-follow-up-in-a-porcine-model
#16
Li Shen, Yizhe Wu, Lei Ge, Yaojun Zhang, Qibing Wang, Juying Qian, Zhifen Qiu, Junbo Ge
We aimed to investigate the safety and efficacy of XINSORB bioresorbable sirolimus-eluting scaffold in porcine model. XINSORB scaffolds and metallic Firebird2™ stents were randomly implanted into minipigs' coronary arteries. Angiography, optical coherent tomography (OCT) and histopathological analyses were performed at post-procedure and 14-, 28-, 90-, 180-day follow-up. Thirty-two minipigs were enrolled. Eight XINSORB scaffolds and 8 Firebird2 stents were examined at each time point. Quantitative coronary angiography showed that in-scaffold late luminal loss (LLL) of XINSORB scaffold was 0...
June 21, 2017: International Journal of Cardiovascular Imaging
https://www.readbyqxmd.com/read/28639027/first-detection-of-enteric-adenoviruses-genotype-41-in-recreation-spring-areas-of-taiwan
#17
Yi-Jia Shih, Chi-Wei Tao, Hsin-Chi Tsai, Wen-Chien Huang, Tung-Yi Huang, Jung-Sheng Chen, Yi-Chou Chiu, Tsui-Kang Hsu, Bing-Mu Hsu
Human adenoviruses (HAdVs) are DNA viruses found in recreational water, such as water parks and swimming pools. Human adenovirus 41 (HAdV-41) is the most common serotype detected and is a leading cause of acute diarrheal disease. The focus of this study is to determine the prevalence of HAdVs in hot springs. Of 57 samples collected from four different geological sites, 16 samples have shown evidence of HAdVs (28.1%). HAdV-41 and porcine adenovirus 5 (PAdV-5) were the two types isolated, with a greater frequency of HAdV-41, which in other settings has been associated with acute diarrhea...
June 22, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28638110/a-novel-and-effective-method-to-generate-human-porcine-specific-regulatory-t-cells-with-high-expression-of-il-10-tgf-%C3%AE-1-and-il-35
#18
Mingqian Li, Judith Eckl, Christiane Geiger, Dolores J Schendel, Heike Pohla
Organ transplantation remains the most effective treatment for patients with late stage organ failure. Transgenic pigs provide an alternative organ donor source to the limited availability of human organs. However, cellular rejection still remains to be the obstacle for xenotransplantation. Superior to other methods, antigen-specific regulatory T cells (Treg) alleviate cellular rejection with fewer side effects. Here we demonstrate the use of a fast method to provide tolerogenic dendritic cells (tolDC) that can be used to generate effective porcine-specific Treg cells (PSTreg)...
June 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28637760/discovery-of-a-highly-divergent-coronavirus-in-the-asian-house-shrew-from-china-illuminates-the-origin-of-the-alphacoronaviruses
#19
Wen Wang, Xian-Dan Lin, Yong Liao, Xiao-Qing Guan, Wen-Ping Guo, Jian-Guang Xing, Edward C Holmes, Yong-Zhen Zhang
Although shrews are one of the largest groups of mammals little is known about their role in the evolution and transmission of viral pathogens including coronaviruses. We captured 266 Asian house shrews (Suncus murinus) in Jiangxi and Zhejiang provinces, China, during 2013-2015. Coronavirus (CoV) RNA was detected in 24 Asian house shrews, with an overall prevalence of 9.02%. Complete viral genome sequences were successfully recovered from the RNA positive samples. The newly discovered shrew CoV fell into four lineages reflecting their geographic origins, indicative of largely allopatric evolution...
June 21, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28637685/milk-oligosaccharides-inhibit-human-rotavirus-infectivity-in-ma104-cells
#20
Daniel R Laucirica, Vassilis Triantis, Ruud Schoemaker, Mary K Estes, Sasirekha Ramani
Background: Oligosaccharides in milk act as soluble decoy receptors and prevent pathogen adhesion to the infant gut. Milk oligosaccharides reduce infectivity of a porcine rotavirus strain; however, the effects on human rotaviruses are less well understood.Objective: In this study, we determined the effect of specific and abundant milk oligosaccharides on the infectivity of 2 globally dominant human rotavirus strains.Methods: Four milk oligosaccharides-2'-fucosyllactose (2'FL), 3'-sialyllactose (3'SL), 6'-sialyllactose (6'SL), and galacto-oligosaccharides-were tested for their effects on the infectivity of human rotaviruses G1P[8] and G2P[4] through fluorescent focus assays on African green monkey kidney epithelial cells (MA104 cells)...
June 21, 2017: Journal of Nutrition
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