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https://www.readbyqxmd.com/read/27922056/identification-and-functional-analysis-of-long-intergenic-noncoding-rna-genes-in-porcine-pre-implantation-embryonic-development
#1
Jingyu Li, Zhengling Gao, Xingyu Wang, Hongbo Liu, Yan Zhang, Zhonghua Liu
Genome-wide transcriptome studies have identified thousands of long intergenic noncoding RNAs (lincRNAs), some of which play important roles in pre-implantation embryonic development (PED). Pig is an ideal model for reproduction, however, porcine lincRNAs are still poorly characterized and it is unknown if they are associated with porcine PED. Here we reconstructed 195,531 transcripts in 122,007 loci, and identified 7,618 novel lincRNAs from 4,776 loci based on published RNA-seq data. These lincRNAs show low exon number, short length, low expression level, tissue-specific expression and cis-acting, which is consistent with previous reports in other species...
December 6, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27922054/first-insight-into-the-proteome-landscape-of-the-porcine-short-posterior-ciliary-arteries-key-signalling-pathways-maintaining-physiologic-functions
#2
Caroline Manicam, Natarajan Perumal, Norbert Pfeiffer, Franz H Grus, Adrian Gericke
Short posterior ciliary arteries (sPCA) provide the major blood supply to the optic nerve head. Emerging evidence has linked structural and functional anomalies of sPCA to the pathogenesis of several ocular disorders that cause varying degrees of visual loss, particularly anterior ischaemic optic neuropathy and glaucoma. Although the functional relevance of this vascular bed is well-recognized, the proteome of sPCA remains uncharacterized. Since the porcine ocular system closely resembles that of the human's and is increasingly employed in translational ophthalmic research, this study characterized the proteome of porcine sPCA employing the mass spectrometry-based proteomics strategy...
December 6, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27921109/dna-and-bsa-damage-inhibitory-activities-and-anti-acetylcholinesterase-anti-porcine-%C3%AE-amylase-and-antioxidant-properties-of-dolichos-lablab-beans
#3
Hosam M Habib, Serah W Theuri, Ehab Kheadr, Fedah E Mohamed
The underutilized Kenyan variety of Dolichos lablab bean seeds serve as a good source of natural antioxidants, which can probably be effective in reducing the risk of occurrence of several diseases. This study was undertaken for the first time to address the limited knowledge regarding the antioxidant activities of lablab beans. Moreover, their DNA damage inhibitory activity, bovine serum albumin (BSA) damage inhibitory activity, and the inhibition of acetylcholinesterase and porcine α-amylase were also investigated...
December 6, 2016: Food & Function
https://www.readbyqxmd.com/read/27920758/effectiveness-of-f18-fimbrial-antigens-released-by-a-novel-autolyzed-salmonella-expression-system-as-a-vaccine-candidate-against-lethal-f18-stec-infection
#4
Gayeon Won, John H Lee
Porcine edema disease (ED) caused by Shiga toxin 2e producing Escherichia coli expressing F18ab(+) fimbriae (F18ab(+)STEC) frequently occurs in post-weaned piglets, resulting in a significant economic loss in swine industries worldwide. In the present study, we proposed an efficient prevention scheme against ED in which the attenuated Salmonella Typhimurium inactivated by the E-mediated cell lysis to deliver target antigens, FedF and FedA, which function in fimbrial-mediated adhesion and as a major subunit of F18ab+fimbriae, respectively...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27920698/a-case-of-en-bloc-excision-of-a-chest-wall-leiomyosarcoma-and-closure-of-the-defect-with-non-cross-linked-collagen-matrix-egis-%C3%A2
#5
Marco Rastrelli, Saveria Tropea, Romina Spina, Alessandra Costa, Roberto Stramare, Simone Mocellin, Maria Giuseppina Bonavina, Carlo Riccardo Rossi
Sarcomas arising from the chest wall account for less than 20% of all soft tissue sarcomas, and at this site, primitive tumors are the most frequent to occur. Leiomyosarcoma is a malignant smooth muscle tumor and the best outcomes are achieved with wide surgical excision. Although advancements have been made in treatment protocols, leiomyosarcoma remains one of the more difficult soft tissue sarcoma to treat. Currently, general local control is obtained with surgical treatment with wide negative margins. We describe the case of a 50-year-old man who underwent a chest wall resection involving a wide portion of the pectoralis major and minor muscle, the serratus and part of the second, third and fourth ribs of the left side...
September 2016: Case Reports in Oncology
https://www.readbyqxmd.com/read/27919816/surface-rearrangement-of-adsorbed-egcg-mucin-complexes-on-hydrophilic-surfaces
#6
J McColl, R Horvath, G E Yakubov, J J Ramsden
The kinetic adsorption-desorption behaviour of porcine gastric mucin in the presence of physiologically relevant concentrations of the polyphenol epigallocatechin gallate (EGCG) was investigated using high-resolution kinetic optical waveguide lightmode spectroscopy (OWLS) and atomic force microscopy (AFM). Comparison with dynamic light scattering results from EGCG-mucin mixtures indicates that discrete particles are formed whose size increases with increasing EGCG:mucin ratio. These particles are deduced to be the adsorbing entities, which fuse on the surface to form complex surface layers...
December 2, 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27919798/femtosecond-laser-assisted-conjunctival-autograft-preparation-for-pterygium-surgery
#7
REVIEW
Matthias Fuest, Yu-Chi Liu, Gary Hin-Fai Yam, Ericia Pei Wen Teo, Hla Myint Htoon, Minas T Coroneo, Jodhbir S Mehta
PURPOSE: Pterygium is a common ocular surface disorder. Conjunctival autografting (CAG) following pterygium resection is the gold standard treatment. CAGs without Tenon's tissue provide better results but are more technically difficult to achieve. In this study, we evaluated the feasibility and reproducibility of femtosecond laser (FSL)-assisted CAG preparation. METHODS: Fifteen porcine globes were fixed in a suction holder and CAGs of different diameters were created by 1) an experienced consultant and 2) a less experienced fellow using the Ziemer LDV Z8...
December 2, 2016: Ocular Surface
https://www.readbyqxmd.com/read/27919740/metabolic-differences-between-cold-stored-and-machine-perfused-porcine-kidneys-a-1-h-nmr-based-study
#8
Jay Nath, Tom B Smith, Kamlesh Patel, Sam R Ebbs, Alex Hollis, Daniel A Tennant, Christian Ludwig, Andrew R Ready
Hypothermic machine perfusion (HMP) and static cold storage (SCS) are the two methods used to preserve deceased donor kidneys prior to transplant. This study seeks to characterise the metabolic profile of HMP and SCS porcine kidneys in a cardiac death donor model. Twenty kidneys were cold flushed and stored for two hours following retrieval. Paired kidneys then underwent 24 h of HMP or SCS or served as time zero controls. Metabolite quantification in both storage fluid and kidney tissue was performed using one dimensional (1)H NMR spectroscopy...
December 2, 2016: Cryobiology
https://www.readbyqxmd.com/read/27919660/a-comparison-of-vasodilation-mode-among-selexipag-ns-304-2-4-5-6-diphenylpyrazin-2-yl-isopropyl-amino-butoxy-n-methylsulfonyl-acetamide-its-active-metabolite-mre-269-and-various-prostacyclin-receptor-agonists-in-rat-porcine-and-human-pulmonary-arteries
#9
Chiaki Fuchikami, Kohji Murakami, Koyuki Tajima, Junko Homan, Keiji Kosugi, Kazuya Kuramoto, Michiko Oka, Keiichi Kuwano
Selexipag (NS-304; [2-{4-[(5,6-diphenylpyrazin-2-yl)(isopropyl)amino]butoxy}-N- (methylsulfonyl)acetamide]) is a novel, orally available non-prostanoid prostacyclin receptor (IP receptor) agonist that has recently been approved for the treatment of pulmonary arterial hypertension (PAH). We examined the effect of the active metabolite of selexipag, MRE-269, and IP receptor agonists that are currently available as PAH therapeutic drugs on the relaxation of rat, porcine and human pulmonary artery. cAMP formation in human pulmonary artery smooth muscle cells was induced by all test compounds (MRE-269, epoprostenol, iloprost, treprostinil and beraprost sodium) and suppressed by IP receptor antagonists (CAY10441 and 2-[4-(1H-indol-4-yloxymethyl)-benzyloxycarbonylamino]-3-phenyl-propionic acid)...
December 2, 2016: European Journal of Pharmacology
https://www.readbyqxmd.com/read/27919634/a-commercial-porcine-circovirus-pcv-type-2a-based-vaccine-reduces-pcv2d-viremia-and-shedding-and-prevents-pcv2d-transmission-to-na%C3%A3-ve-pigs-under-experimental-conditions
#10
Tanja Opriessnig, Chao-Ting Xiao, Patrick G Halbur, Priscilla F Gerber, Shannon R Matzinger, Xiang-Jin Meng
Porcine circovirus type 2 (PCV2) vaccination has been effective in protecting pigs from clinical disease and today is used extensively. Recent studies in vaccinated populations indicate a major PCV2 genotype shift from the predominant PCV2 genotype 2b towards 2d. The aims of this study were to determine the ability of the commercial inactivated PCV2a vaccine Circovac® to protect pigs against experimental challenge with a 2013 PCV2d strain and prevent transmission. Thirty-eight pigs were randomly divided into four groups with 9-10 pigs per group: NEG (sham-vaccinated, sham-challenged), VAC (PCV2a-vaccinated, sham-challenged), VAC+CHAL (PCV2a-vaccinated and PCV2d-challenged), and CHAL (sham-vaccinated, PCV2d-challenged)...
December 2, 2016: Vaccine
https://www.readbyqxmd.com/read/27918009/optical-properties-of-porcine-dermis-in-the-mid-infrared-absorption-band-of-glucose
#11
Arthur Schönhals, Hans Tholl, Mathias Glasmacher, Niels Kröger-Lui, Annemarie Pucci, Wolfgang Petrich
The optical properties of skin in the mid-infrared range are not known, despite their importance for e.g. non-invasive glucose monitoring. In this paper, transmission, absorption, scattering, and reduced scattering coefficients are quantified using a custom-built goniometer based on a quantum cascade laser operated at the glucose absorption band at a wavelength of around 9.7 μm. The measurements show a strong dominance of absorption and moderate contributions from scattering. The scattered radiation is dominated by single scattering events in the forward direction (g = 0...
December 5, 2016: Analyst
https://www.readbyqxmd.com/read/27917649/percutaneous-externally-assembled-laparoscopic-peal-versus-laparoendoscopic-single-site-less-nephrectomy-in-a-porcine-model-a-prospective-randomized-blinded-study
#12
Mohamed Keheila, Jim Ken-Chie Shen, Daniel Faaborg, Patrick Yang, Salim Cheriyan, Samuel R Abourbih, Nazih Khater, Michael Hill, D Duane Baldwin
OBJECTIVE: To compare the outcomes between Laparoendoscopic single-site (LESS) and a novel Percutaneous Externally Assembled Laparoscopic (PEAL) nephrectomy in an in vivo porcine model. MATERIALS AND METHODS: Ten female farm pigs were randomized to LESS nephrectomy (5) or PEAL nephrectomy (5). Operative times, estimated blood loss, intraoperative and postoperative complications were compared. The surgeons used a Likert scale to grade difficulty of peritoneal access, port placement, tool assembly, hilar dissection, closure, and overall difficulty of surgery...
December 4, 2016: Journal of Endourology
https://www.readbyqxmd.com/read/27917461/intussusceptive-angiogenesis-and-expression-of-tie-receptors-during-porcine-metanephric-kidney-development
#13
Anastasia Logothetidou, Tim Vandecasteele, Els Van Mulken, Kimberley Vandevelde, Pieter Cornillie
Intussusceptive angiogenesis (IA) is required for normal embryonic vascular development. The Tie family of receptors and their ligands, the angiopoietins, play an important role in the growth or regression of blood vessels which are important not only during development but also throughout an organism's life. The presence of IA was investigated in glomerular capillaries of the fetal porcine metanephros using Mercox II resin casts. The first signs of IA were observed in stage III glomeruli. Stage IV and V glomeruli showed numerous signs of aligned pillar formation and their successive merging to delineate the vascular entities...
December 5, 2016: Histology and Histopathology
https://www.readbyqxmd.com/read/27916855/comparative-proteome-analysis-of-porcine-jejunum-tissues-in-response-to-a-virulent-strain-of-porcine-epidemic-diarrhea-virus-and-its-attenuated-strain
#14
Zhonghua Li, Fangzhou Chen, Shiyi Ye, Xiaozhen Guo, Atta Muhanmmad Memon, Meizhou Wu, Qigai He
Porcine epidemic diarrhea virus (PEDV), a predominant cause of acute enteric infection, leads to severe dehydrating diarrhea and mortality in piglets all over the world. A virulent PEDV YN13 strain, isolated in our laboratory, was attenuated to yield an attenuated PEDV strain YN144. To better understand the pathogenesis mechanism and the virus-host interaction during infection with both PEDV YN13 and YN144 strains, a comparative proteomic analysis was carried out to investigate the proteomic changes produced in the primary target organ, using isobaric tags for relative and absolute quantitation (iTRAQ) labeling, followed by liquid chromatography tandem-mass spectrometry (LC-MS/MS)...
November 29, 2016: Viruses
https://www.readbyqxmd.com/read/27916787/comprehensive-mirna-expression-profiles-in-the-ilea-of-lawsonia-intracellularis-infected-pigs
#15
Hongyi Li, Mao Zhang, Enqin Zheng
To analyze the miRNA expression profiles in Lawsoniaintracellularis-infected porcine intestines, infected pigs were first identified using PCR (Polymerase Chain Reaction). Then, RNA from infected intestines and control tissues were isolated and subjected to microarray analysis and RT-PCR. Results showed that a total of 83 miRNAs were differentially expressed between the infected samples and controls, out of which 53 were upregulated and 30 were downregulated. Validation using RT-PCR showed a high degree of confidence for the obtained data...
December 4, 2016: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/27916002/long-term-effect-of-stents-eluting-6-mercaptopurine-in-porcine-coronary-arteries
#16
Matthijs S Ruiter, Albert Doornbos, Vivian de Waard, Robbert J de Winter, Nico J M Attevelt, Rob Steendam, Carlie J M de Vries
BACKGROUND: Drug-eluting stents (DES) have dramatically reduced restenosis rates compared to bare metal stents and are widely used in coronary artery angioplasty. The anti-proliferative nature of the drugs reduces smooth muscle cell (SMC) proliferation effectively, but unfortunately also negatively affects endothelialization of stent struts, necessitating prolonged dual anti-platelet therapy. Cell-type specific therapy may prevent this complication, giving rise to safer stents that do not require additional medication...
December 5, 2016: Journal of Negative Results in Biomedicine
https://www.readbyqxmd.com/read/27915587/the-determination-of-testosterone-esters-and-estradiol-esters-in-bovine-and-porcine-blood-serum
#17
Martina Rejtharová, Libor Rejthar, Katarína Čačková
Monitoring of steroid esters in blood serum is desirable in order to detect possible illegal use of natural hormones as growth promoters. A method for determination of testosterone propionate, testosterone benzoate, testosterone isocaproate, testosterone decanoate and estradiol benzoate in bovine and porcine blood serum was developed. The procedure consists of protein precipitation and removal of phospholipids using a HybridSPE®-Phospholipid column followed by clean-up on a hydrophilic modified styrene polymer Supel(TM) -Select HLB column and LC-MS/MS measurement...
December 5, 2016: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
https://www.readbyqxmd.com/read/27915442/co-exposure-to-low-doses-of-the-food-contaminants-deoxynivalenol-and-nivalenol-has-a-synergistic-inflammatory-effect-on-intestinal-explants
#18
Imourana Alassane-Kpembi, Olivier Puel, Philippe Pinton, Anne-Marie Cossalter, Ting-Chao Chou, Isabelle P Oswald
The global incidence of Fusarium head blight and attendant cereal grains multi-contamination by the trichothecene mycotoxins deoxynivalenol (DON) and nivalenol (NIV) are increasing as a possible result of climate change and inadequate agricultural practices. At the molecular level, these mycotoxins bind to the ribosome, activate the mitogen-activated protein kinase and induce a local and systemic inflammation. DON is of public health concern owing to the narrow margin between exposure and tolerable daily intake...
December 3, 2016: Archives of Toxicology
https://www.readbyqxmd.com/read/27915189/a-test-of-the-citrate-method-of-pmi-estimation-from-skeletal-remains
#19
Sarah J Wilson, Angi M Christensen
Citrate content in bone has been shown to be associated with the postmortem interval (PMI), with citrate decreasing after death as a function of time. Here we test this method using porcine ribs for the period of 1-165days after death, and also assess citrate content and variation from samples placed into two different postmortem environments (terrestrial and aquatic). Higher citrate variation, lower citrate recovery, and a weaker association with time were found in this study as compared to others. Citrate content, however, was found to decrease with increasing PMI, and the method was found to be easy and inexpensive to apply...
November 24, 2016: Forensic Science International
https://www.readbyqxmd.com/read/27915108/how-should-we-respond-to-the-emergence-of-plasmid-mediated-colistin-resistance-in-humans-and-animals
#20
REVIEW
Jaffar A Al-Tawfiq, Ramanan Laxminarayan, Marc Mendelson
OBJECTIVE: The widespread use of antibiotics in humans and animals contributes to growing rates of antibiotic resistance. Previously treatable bacterial infections now require the last line of antibiotics or are untreatable. For carbapenem-resistant, Gram-negative bacterial infections the antibiotic of last resort is now often colistin. In this review, we present the evidence for the shifting pattern of colistin resistance and discuss how the international community should respond. METHODS: This is a literature review of colistin resistance...
November 30, 2016: International Journal of Infectious Diseases: IJID
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