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https://www.readbyqxmd.com/read/28654647/ancient-female-philopatry-asymmetric-male-gene-flow-and-synchronous-population-expansion-support-the-influence-of-climatic-oscillations-on-the-evolution-of-south-american-sea-lion-otaria-flavescens
#1
Larissa Rosa de Oliveira, Marcelo C M Gehara, Lúcia D Fraga, Fernando Lopes, Juan Ignacio Túnez, Marcelo H Cassini, Patricia Majluf, Susana Cárdenas-Alayza, Héctor J Pavés, Enrique Alberto Crespo, Nestor García, Rocío Loizaga de Castro, A Rus Hoelzel, Maritza Sepúlveda, Carlos Olavarría, Victor Hugo Valiati, Renato Quiñones, Maria Jose Pérez-Alvarez, Paulo Henrique Ott, Sandro L Bonatto
The South American sea lion (Otaria flavescens) is widely distributed along the southern Atlantic and Pacific coasts of South America with a history of significant commercial exploitation. We aimed to evaluate the population genetic structure and the evolutionary history of South American sea lion along its distribution by analyses of mitochondrial DNA (mtDNA) and 10 nuclear microsatellites loci. We analyzed 147 sequences of mtDNA control region and genotyped 111 individuals of South American sea lion for 10 microsatellite loci, representing six populations (Peru, Northern Chile, Southern Chile, Uruguay (Brazil), Argentina and Falkland (Malvinas) Islands) and covering the entire distribution of the species...
2017: PloS One
https://www.readbyqxmd.com/read/28640659/phlda1-pleckstrin-homology-like-domain-family-a-member-1-knockdown-promotes-migration-and-invasion-of-mcf10a-breast-epithelial-cells
#2
Naieli Bonatto, Maria José Carlini, Simone Aparecida de Bessa Garcia, Maria Aparecida Nagai
PHLDA1 (pleckstrin homology-like domain, family A, member 1) is a multifunctional protein that plays distinct roles in several biological processes including cell death and therefore its altered expression has been identified in different types of cancer. Progressively loss of PHLDA1 was found in primary and metastatic melanoma while its overexpression was reported in intestinal and pancreatic tumors. Previous work from our group showed that negative expression of PHLDA1 protein was a strong predictor of poor prognosis for breast cancer disease...
June 22, 2017: Cell Adhesion & Migration
https://www.readbyqxmd.com/read/28562344/accumulation-of-prohibitin-is-a-common-cellular-response-to-different-stressing-stimuli-and-protects-melanoma-cells-from-er-stress-and-chemotherapy-induced-cell-death
#3
Tharcisio Citrangulo Tortelli, Lyris Martins Franco de Godoy, Gustavo Antonio de Souza, Diego Bonatto, Andreia Hanada Otake, Renata de Freitas Saito, Jose Cesar Rosa, Lewis Joel Greene, Roger Chammas
Melanoma is responsible for most deaths among skin cancers and conventional and palliative care chemotherapy are limited due to the development of chemoresistance. We used proteomic analysis to identify cellular responses that lead to chemoresistance of human melanoma cell lines to cisplatin. A systems approach to the proteomic data indicated the participation of specific cellular processes such as oxidative phosphorylation, mitochondrial organization and homeostasis, as well as the unfolded protein response (UPR) to be required for the survival of cells treated with cisplatin...
June 27, 2017: Oncotarget
https://www.readbyqxmd.com/read/28545114/different-adaptation-strategies-of-two-citrus-scion-rootstock-combinations-in-response-to-drought-stress
#4
Joadson Dutra de Souza, Edson Mario de Andrade Silva, Mauricio Antônio Coelho Filho, Raphaël Morillon, Diego Bonatto, Fabienne Micheli, Abelmon da Silva Gesteira
Scion/rootstock interaction is important for plant development and for breeding programs. In this context, polyploid rootstocks presented several advantages, mainly in relation to biotic and abiotic stresses. Here we analyzed the response to drought of two different scion/rootstock combinations presenting different polyploidy: the diploid (2x) and autotetraploid (4x) Rangpur lime (Citrus limonia, Osbeck) rootstocks grafted with 2x Valencia Delta sweet orange (Citrus sinensis) scions, named V/2xRL and V/4xRL, respectively...
2017: PloS One
https://www.readbyqxmd.com/read/28340499/pagan-symbols-associated-with-the-female-anatomy-in-the-medici-chapel-by-michelangelo-buonarroti
#5
Deivis De Campos, Andrea Oxley Da Rocha, Rodrigo De Oliveira Lemos, Tais Malysz, João Antonio Bonatto-Costa, Geraldo Pereira Jotz, Lino Pinto De Oliveira Junior, Miriam Da Costa Oliveira
Numerous studies have shown that many works of art from the Renaissance period contain hidden symbols and codes that could have religious, mathematical and/or pagan significance and even anatomical allusions. In this context, the present manuscript offers new evidence that the great genius of anatomy, Michelangelo Buonarroti (1475-1564), included pagan symbols associated with female anatomy in the funerary monuments found in the Sagrestia Nuova/Medici Chapel (1519-1533) in Florence, Italy. The interpretation of the symbols provided in this study will interest those with a passion for the history of anatomy...
March 24, 2017: Clinical Anatomy
https://www.readbyqxmd.com/read/28199443/secondary-structure-of-nrdna-internal-transcribed-spacers-as-a-useful-tool-to-align-highly-divergent-species-in-phylogenetic-studies
#6
Giovanna C Giudicelli, Geraldo Mäder, Gustavo A Silva-Arias, Priscilla M Zamberlan, Sandro L Bonatto, Loreta B Freitas
Recently, it has been suggested that internal transcribed spacer (ITS) sequences are under selective constraints to preserve their secondary structure. Here, we investigate the patterns of the ITS nucleotide and secondary structure conservation across the Passiflora L. genus to evaluate the potential use of secondary structure data as a helpful tool for the alignment in taxonomically complex genera. Considering the frequent use of ITS, this study also presents a perspective on future analyses in other plant groups...
2017: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/28174095/anti-mitotic-agents-are-they-emerging-molecules-for-cancer-treatment
#7
REVIEW
Larissa Siqueira Penna, João Antonio Pêgas Henriques, Diego Bonatto
Mutations in cancer cells frequently result in cell cycle alterations that lead to unrestricted growth compared to normal cells. Considering this phenomenon, many drugs have been developed to inhibit different cell-cycle phases. Mitotic phase targeting disturbs mitosis in tumor cells, triggers the spindle assembly checkpoint and frequently results in cell death. The first anti-mitotics to enter clinical trials aimed to target tubulin. Although these drugs improved the treatment of certain cancers, and many anti-microtubule compounds are already approved for clinical use, severe adverse events such as neuropathies were observed...
May 2017: Pharmacology & Therapeutics
https://www.readbyqxmd.com/read/28060024/stress-distribution-in-an-implant-supported-mandibular-complete-denture-using-different-cantilever-lengths-and-occlusal-coating-materials
#8
Rodrigo Antonio de Medeiros, Marcelo Coelho Goiato, Aldiéris Alves Pesqueira, Aljomar José Vechiato Filho, Liliane da Rocha Bonatto, Daniela Micheline Dos Santos
PURPOSE: The aim of this study was to assess stress distribution in the bone-implant interface of a mandibular implant-supported prosthesis with different cantilever lengths, aesthetic coating materials, and implant abutments. MATERIALS AND METHODS: A photoelastic model of an edentulous mandible, containing 5 external hexagon implants, was constructed. Experimental models were divided into 6 groups: group 1-UCLA component and metal bar; group 2-UCLA component and acrylic resin coating; group 3-UCLA component and porcelain coating; group 4-abutment and metal bar; group 5-abutment and acrylic resin coating; and group 6-abutment and porcelain coating...
February 2017: Implant Dentistry
https://www.readbyqxmd.com/read/27987770/surface-characterization-of-polymers-used-in-fabrication-of-interim-prostheses-after-treatment-with-photopolymerized-glaze
#9
Daniela Micheline Dos Santos, Betina Chiarelo Commar, Liliane da Rocha Bonatto, Emily Vivianne Freitas da Silva, Mariana Vilela Sônego, Elidiane Cipriano Rangel, Aldieris Alves Pesqueira, Marcelo Coelho Goiato
The material used for interim prostheses fabrication must present excellent physical properties for greater longevity in the face of environmental conditions, which can occur in the oral cavity. The purpose of this study was to evaluate the effect of a photopolymerized glaze on the physical and mechanical properties of polymers used for the fabrication of interim prostheses, before and after thermocycling and immersion in staining solutions. One hundred samples of composite and acrylic resins were fabricated: Dencor chemically activated acrylic resin (CAAR) (n=20) and heat-polymerized acrylic resin (HPAR) (n=20), Charisma (n=20), Structur (n=20), and Protemp (n=20)...
February 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/27916542/the-role-of-the-ubiquitin-proteasome-system-in-the-memory-process
#10
REVIEW
Philomena Z Y Lip, Marilene Demasi, Diego Bonatto
Quite intuitive is the notion that memory formation and consolidation is orchestrated by protein synthesis because of the synaptic plasticity necessary for those processes. Nevertheless, recent advances have begun accumulating evidences of a high requirement for protein degradation on the molecular mechanisms of the memory process in the mammalian brain. Because degradation determines protein half-life, degradation has been increasingly recognized as an important intracellular regulatory mechanism. The proteasome is the main player in the degradation of intracellular proteins...
December 2, 2016: Neurochemistry International
https://www.readbyqxmd.com/read/27836141/biocompatibility-of-primers-and-an-adhesive-used-for-implant-retained-maxillofacial-prostheses-an-in%C3%A2-vitro-analysis
#11
Liliane da Rocha Bonatto, Marcelo Coelho Goiato, Emily Vivianne Freitas da Silva, Sandra Helena Penha Oliveira, Marcela Filié Haddad, Antônio Hernandes Chaves Neto, Victor Gustavo Balera Brito, Daniela Micheline Dos Santos
STATEMENT OF PROBLEM: Implant-retained maxillofacial prostheses should be biocompatible, regardless of the primers and adhesives used to bond the acrylic resin and facial silicone. The authors are unaware of any study evaluating the influence of these primers and adhesives on the biocompatibility of maxillofacial prostheses. PURPOSE: The purpose of this in vitro study was to evaluate the cytotoxic effect of primers and an adhesive used to bond acrylic resin and facial silicone during the fabrication of implant-retained maxillofacial prostheses...
June 2017: Journal of Prosthetic Dentistry
https://www.readbyqxmd.com/read/27612795/in-vitro-analysis-of-different-properties-of-acrylic-resins-for-ocular-prosthesis-submitted-to-accelerated-aging-with-or-without-photopolymerized-glaze
#12
Daniela Micheline Dos Santos, Bruna Egumi Nagay, Emily Vivianne Freitas da Silva, Liliane da Rocha Bonatto, Mariana Vilela Sonego, Amália Moreno, Elidiane Cipriano Rangel, Nilson Cristino da Cruz, Marcelo Coelho Goiato
The effect of a photopolymerized glaze on different properties of acrylic resin (AR) for ocular prostheses submitted to accelerated aging was investigated. Forty discs were divided into 4 groups: N1 AR without glaze (G1); colorless AR without glaze (G2); N1 AR with glaze (G3); and colorless AR with glaze (G4). All samples were polished with sandpaper (240, 600 and 800-grit). In G1 and G2, a 1200-grit sandpaper was also used. In G3 and G4, samples were coated with MegaSeal glaze. Property analysis of color stability, microhardness, roughness, and surface energy, and assays of atomic force microscopy, scanning electron microscopy, and energy-dispersive spectroscopy were performed before and after the accelerated aging (1008h)...
December 1, 2016: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/27556094/endoscopic-evaluation-of-celiac-disease-severity-and-its-correlation-with-histopathological-aspects-of-the-duodenal-mucosa
#13
Mauro W Bonatto, Luiz Kotze, Marcia Orlandoski, Ricardo Tsuchyia, Carlos A de Carvalho, Doryane Lima, Gustavo Kurachi, Ivan R B Orso, Lorete Kotze
BACKGROUND AND STUDY AIMS: Celiac disease (CD) is a chronic systemic autoimmune disorder affecting genetically predisposed individuals, triggered and maintained by the ingestion of gluten. Triggered and maintained by the ingestion of gluten, celiac disease is a chronic systemic autoimmune disorder affecting genetically predisposed individuals. Persistent related inflammation of the duodenal mucosa causes atrophy architecture detectable on esophagogastroduodenoscopy (EGD) and histopathology...
July 2016: Endoscopy International Open
https://www.readbyqxmd.com/read/27521695/toxicity-analysis-of-ocular-prosthesis-acrylic-resin-with-or-without-pigment-incorporation-in-human-conjunctival-cell-line
#14
Emily Vivianne Freitas da Silva, Marcelo Coelho Goiato, Liliane da Rocha Bonatto, Rodrigo Antonio de Medeiros, Daniela Micheline Dos Santos, Elidiane Cipriano Rangel, Sandra Helena Penha de Oliveira
The aim of this study was to evaluate the influence of pigment incorporation on the cytotoxicity of ocular prosthesis N1 color acrylic resin. Nine samples were manufactured by heat-polymerization in water bath and divided into 3 groups: acrylic resin without pigment incorporation (group R), acrylic resin with pigment incorporation (group RP), and acrylic pigment (group P). Eluates formed after 72h of sample immersion in medium were incubated with conjunctival cell line (Chang conjunctival cells) for 72h. The negative control group consisted in medium without samples (group C)...
October 2016: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/27501255/the-hidden-symbols-of-the-female-anatomy-in-michelangelo-buonarroti-s-ceiling-in-the-sistine-chapel
#15
Deivis de Campos, Tais Malysz, João Antonio Bonatto-Costa, Geraldo Pereira Jotz, Lino Pinto de Oliveira Junior, Jéssica Francine Wichmann, Guilherme Reghelin Goulart, Marco Antonio Stefani, Andrea Oxley da Rocha
A number of published articles have suggested that each element of Renaissance art contains an inner meaning. Some of these elements include the choice of theme and protagonists, faces selected for the characters, colors used, species of flowers and trees chosen, animals depicted, positions of the elements, posture of the characters and their gestures, juxtapositions in the scenes, and even the very scenario or landscape. All of these elements are thought to have hidden meanings. In this context, this manuscript presents a new hypothesis suggesting that Michelangelo Buonarroti (1475-1564) may have concealed symbols associated with female anatomy in the ceiling of the Sistine Chapel (painted 1508-1512) in Rome...
October 2016: Clinical Anatomy
https://www.readbyqxmd.com/read/27438419/recurrence-rate-of-helicobacter-pylori-in-patients-with-peptic-ulcer-five-years-or-more-after-successful-eradication
#16
Yuri Costa Farago Fernandes, Gabriel da Rocha Bonatto, Mauro Willeman Bonatto
BACKGROUND: Infection with Helicobacter pylori is highly prevalent worldwide, especially in developing countries. Its presence in the gastroduodenal mucosa is related with development of peptic ulcer and other illnesses. The eradication of H. pylori improves mucosal histology in patients with peptic ulcers. OBJECTIVE: This study was aimed to verify if H. pylori recurrence occurs five years or more after confirmed eradication in patients with peptic ulcer. Moreover, we sought to determine the recurrence rate...
July 2016: Arquivos de Gastroenterologia
https://www.readbyqxmd.com/read/27422225/effect-of-different-methods-of-polymerizing-ocular-prosthesis-acrylic-resin-on-a-human-conjunctival-cell-line
#17
Emily Vivianne Freitas da Silva, Marcelo Coelho Goiato, Daniela Micheline Dos Santos, Liliane da Rocha Bonatto, Victor Gustavo Balera Brito, Sandra Helena Penha de Oliveira
STATEMENT OF PROBLEM: Ocular prosthesis acrylic resins should be biocompatible regardless of the polymerization method. The authors are unaware of a study that evaluated the biocompatibility of ocular prostheses. PURPOSE: The purpose of this in vitro study was to evaluate the cytotoxicity of different methods of polymerizing ocular prosthesis acrylic resin. This was accomplished by analyzing the cell proliferation, production of proinflammatory cytokines, and expression of extracellular matrix proteins related to tissue remodeling and repair of a human conjunctival cell line...
November 2016: Journal of Prosthetic Dentistry
https://www.readbyqxmd.com/read/27409230/reproductive-parameters-of-phytoseiulus-macropilis-banks-fed-with-tetranychus-urticae-koch-acari-phytoseiidae-tetranychidae-in-laboratory
#18
G C Souza-Pimentel, P R Reis, C R Bonatto, J P Alves, M F Siqueira
Predatory mites that belong to the Phytoseiidae family are one of the main natural enemies of phytophagous mites, thus allowing for their use as a biological control. Phytoseiulus macropilis (Banks, 1904) (Acari: Phytoseiidae) is among the main species of predatory mites used for this purpose. Tetranychus urticae Koch, 1836 (Acari: Tetranychidae) is considered to be one of the most important species of mite pests and has been described as attacking over 1,100 species of plants in 140 families with economic value...
July 11, 2016: Brazilian Journal of Biology, Revista Brasleira de Biologia
https://www.readbyqxmd.com/read/27233438/multiple-evolutionary-units-and-demographic-stability-during-the-last-glacial-maximum-in-the-scytalopus-speluncae-complex-aves-rhinocryptidae
#19
Paola Pulido-Santacruz, Marcos Ricardo Bornschein, Ricardo Belmonte-Lopes, Sandro L Bonatto
The Atlantic Forest (AF) of South America harbors one of the world's highest bird species richness, but to date there is a deficient understanding of the spatial patterns of genetic diversity and the evolutionary history of this biome. Here we estimated the phylogenetic and populational history of the widespread Mouse-colored Tapaculo (Scytalopus speluncae) complex across the Brazilian AF, using data from two mitochondrial genes and 12 microsatellite loci. Both markers uncovered several cryptic, mostly allopatric and well-supported lineages that may represent distinct species-level taxa...
September 2016: Molecular Phylogenetics and Evolution
https://www.readbyqxmd.com/read/27188539/could-refuge-theory-and-rivers-acting-as-barriers-explain-the-genetic-variability-distribution-in-the-atlantic-forest
#20
Ana Luiza R Cazé, Geraldo Mäder, Teonildes S Nunes, Luciano P Queiroz, Guilherme de Oliveira, José Alexandre F Diniz-Filho, Sandro L Bonatto, Loreta B Freitas
The Atlantic Forest is one of the most species-rich ecoregions in the world. The historical origins of this richness and the evolutionary processes that produced diversification and promoted speciation in this ecosystem remain poorly understood. In this context, focusing on Passiflora contracta, an endemic species from the Atlantic Forest distributed exclusively at sea level along forest edges, this study aimed to characterize the patterns of genetic variability and explore two hypotheses that attempt to explain the possible causes of the genetic diversity in this region: the refuge and riverine barrier theories...
August 2016: Molecular Phylogenetics and Evolution
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