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Daniela Micheline Dos Santos, Bruna Egumi Nagay, Emily Vivianne Freitas da Silva, Liliane da Rocha Bonatto, Mariana Vilela Sonego, Amália Moreno, Elidiane Cipriano Rangel, Nilson Cristino da Cruz, Marcelo Coelho Goiato
The effect of a photopolymerized glaze on different properties of acrylic resin (AR) for ocular prostheses submitted to accelerated aging was investigated. Forty discs were divided into 4 groups: N1 AR without glaze (G1); colorless AR without glaze (G2); N1 AR with glaze (G3); and colorless AR with glaze (G4). All samples were polished with sandpaper (240, 600 and 800-grit). In G1 and G2, a 1200-grit sandpaper was also used. In G3 and G4, samples were coated with MegaSeal glaze. Property analysis of color stability, microhardness, roughness, and surface energy, and assays of atomic force microscopy, scanning electron microscopy, and energy-dispersive spectroscopy were performed before and after the accelerated aging (1008h)...
December 1, 2016: Materials Science & Engineering. C, Materials for Biological Applications
Mauro W Bonatto, Luiz Kotze, Marcia Orlandoski, Ricardo Tsuchyia, Carlos A de Carvalho, Doryane Lima, Gustavo Kurachi, Ivan R B Orso, Lorete Kotze
BACKGROUND AND STUDY AIMS: Celiac disease (CD) is a chronic systemic autoimmune disorder affecting genetically predisposed individuals, triggered and maintained by the ingestion of gluten. Triggered and maintained by the ingestion of gluten, celiac disease is a chronic systemic autoimmune disorder affecting genetically predisposed individuals. Persistent related inflammation of the duodenal mucosa causes atrophy architecture detectable on esophagogastroduodenoscopy (EGD) and histopathology...
July 2016: Endoscopy International Open
Emily Vivianne Freitas da Silva, Marcelo Coelho Goiato, Liliane da Rocha Bonatto, Rodrigo Antonio de Medeiros, Daniela Micheline Dos Santos, Elidiane Cipriano Rangel, Sandra Helena Penha de Oliveira
The aim of this study was to evaluate the influence of pigment incorporation on the cytotoxicity of ocular prosthesis N1 color acrylic resin. Nine samples were manufactured by heat-polymerization in water bath and divided into 3 groups: acrylic resin without pigment incorporation (group R), acrylic resin with pigment incorporation (group RP), and acrylic pigment (group P). Eluates formed after 72h of sample immersion in medium were incubated with conjunctival cell line (Chang conjunctival cells) for 72h. The negative control group consisted in medium without samples (group C)...
October 2016: Toxicology in Vitro: An International Journal Published in Association with BIBRA
Deivis de Campos, Tais Malysz, João Antonio Bonatto-Costa, Geraldo Pereira Jotz, Lino Pinto de Oliveira Junior, Jéssica Francine Wichmann, Guilherme Reghelin Goulart, Marco Antonio Stefani, Andrea Oxley da Rocha
A number of published articles have suggested that each element of Renaissance art contains an inner meaning. Some of these elements include the choice of theme and protagonists, faces selected for the characters, colors used, species of flowers and trees chosen, animals depicted, positions of the elements, posture of the characters and their gestures, juxtapositions in the scenes, and even the very scenario or landscape. All of these elements are thought to have hidden meanings. In this context, this manuscript presents a new hypothesis suggesting that Michelangelo Buonarroti (1475-1564) may have concealed symbols associated with female anatomy in the ceiling of the Sistine Chapel (painted 1508-1512) in Rome...
October 2016: Clinical Anatomy
Yuri Costa Farago Fernandes, Gabriel da Rocha Bonatto, Mauro Willeman Bonatto
BACKGROUND: Infection with Helicobacter pylori is highly prevalent worldwide, especially in developing countries. Its presence in the gastroduodenal mucosa is related with development of peptic ulcer and other illnesses. The eradication of H. pylori improves mucosal histology in patients with peptic ulcers. OBJECTIVE: This study was aimed to verify if H. pylori recurrence occurs five years or more after confirmed eradication in patients with peptic ulcer. Moreover, we sought to determine the recurrence rate...
July 2016: Arquivos de Gastroenterologia
Emily Vivianne Freitas da Silva, Marcelo Coelho Goiato, Daniela Micheline Dos Santos, Liliane da Rocha Bonatto, Victor Gustavo Balera Brito, Sandra Helena Penha de Oliveira
STATEMENT OF PROBLEM: Ocular prosthesis acrylic resins should be biocompatible regardless of the polymerization method. No study has evaluated the biocompatibility of ocular prostheses. PURPOSE: The purpose of this in vitro study was to evaluate the cytotoxicity of different methods of polymerizing ocular prosthesis acrylic resin. This was accomplished by analyzing the cell proliferation, production of proinflammatory cytokines, and expression of extracellular matrix proteins related to tissue remodeling and repair of a human conjunctival cell line...
July 13, 2016: Journal of Prosthetic Dentistry
G C Souza-Pimentel, P R Reis, C R Bonatto, J P Alves, M F Siqueira
Predatory mites that belong to the Phytoseiidae family are one of the main natural enemies of phytophagous mites, thus allowing for their use as a biological control. Phytoseiulus macropilis (Banks, 1904) (Acari: Phytoseiidae) is among the main species of predatory mites used for this purpose. Tetranychus urticae Koch, 1836 (Acari: Tetranychidae) is considered to be one of the most important species of mite pests and has been described as attacking over 1,100 species of plants in 140 families with economic value...
July 11, 2016: Brazilian Journal of Biology, Revista Brasleira de Biologia
Paola Pulido-Santacruz, Marcos Ricardo Bornschein, Ricardo Belmonte-Lopes, Sandro L Bonatto
The Atlantic Forest (AF) of South America harbors one of the world's highest bird species richness, but to date there is a deficient understanding of the spatial patterns of genetic diversity and the evolutionary history of this biome. Here we estimated the phylogenetic and populational history of the widespread Mouse-colored Tapaculo (Scytalopus speluncae) complex across the Brazilian AF, using data from two mitochondrial genes and 12 microsatellite loci. Both markers uncovered several cryptic, mostly allopatric and well-supported lineages that may represent distinct species-level taxa...
September 2016: Molecular Phylogenetics and Evolution
Ana Luiza R Cazé, Geraldo Mäder, Teonildes S Nunes, Luciano P Queiroz, Guilherme de Oliveira, José Alexandre F Diniz-Filho, Sandro L Bonatto, Loreta B Freitas
The Atlantic Forest is one of the most species-rich ecoregions in the world. The historical origins of this richness and the evolutionary processes that produced diversification and promoted speciation in this ecosystem remain poorly understood. In this context, focusing on Passiflora contracta, an endemic species from the Atlantic Forest distributed exclusively at sea level along forest edges, this study aimed to characterize the patterns of genetic variability and explore two hypotheses that attempt to explain the possible causes of the genetic diversity in this region: the refuge and riverine barrier theories...
August 2016: Molecular Phylogenetics and Evolution
Ulrich Ulmer, Martin Cholewa, Thomas Diemant, Christian Bonatto Minella, Roland Dittmeyer, R Jürgen Behm, Maximilian Fichtner
A study of the reactions of liquid acetone and toluene on transition metal hydrides, which can be used in thermal energy or hydrogen storage applications, is presented. Hydrogen is confined in TiFe, Ti0.95Zr0.05Mn1.49V0.45Fe0.06 ("Hydralloy C5"), and V40Fe8Ti26Cr26 after contact with acetone. Toluene passivates V40Fe8Ti26Cr26 completely for hydrogen desorption while TiFe is only mildly deactivated and desorption is not blocked at all in the case of Hydralloy C5. LaNi5 is inert toward both organic liquids. Gas chromatography (GC) investigations reveal that CO, propane, and propene are formed during hydrogen desorption from V40Fe8Ti26Cr26 in liquid acetone, and methylcyclohexane is formed in the case of liquid toluene...
June 8, 2016: ACS Applied Materials & Interfaces
Samyra Jogaib Bonatto, Mariana Kerner, Saulo Merelles, Milena Pereira Pondé
This study aims to estimate the prevalence of symptoms of attention-deficit/hyperactivity disorder (ADHD) in parents of children with autism spectrum disorder (ASD). This is a cross-sectional study conducted with the parents of 89 children previously diagnosed with ASD. The research instrument used was the 18-item Adult ADHD Self-Report Scale (ASRS). Symptoms of ADHD were present in 10.4% of the mothers of children with a diagnosis of ASD and in 11.3% of the fathers. These results suggest that the prevalence of symptoms of ADHD in the parents of children with autism is higher than that found in the general adult population...
June 30, 2016: Psychiatry Research
Geraldo Mäder, Luana Castro, Sandro Luis Bonatto, Loreta Brandão de Freitas
Non-indigenous plants exhibit different attributes that make them aggressive competitors with indigenous plants and serious threats to biodiversity.Senecio madagascariensis (fireweed, Asteraceae), a native from southern Africa, is a strong competitor in agricultural activities and has toxic alkaloids that may result in high cattle mortality. In Brazil, this weed was collected for the first time in 1995 and has since spread quickly throughout the Pampas region. To better understand the invasion of the fireweed in South America, we used a genetic characterization with internal transcribed spacer (ITS) and microsatellite markers...
March 2016: Genetics and Molecular Biology
Mauricio Batista Fialho, Alexander de Andrade, José Matheus Camargo Bonatto, Fernanda Salvato, Carlos Alberto Labate, Sérgio Florentino Pascholati
Volatile organic compounds (VOCs) released by Saccharomyces cerevisiae inhibit plant pathogens, including the filamentous fungus Phyllosticta citricarpa, causal agent of citrus black spot. VOCs mediate relevant interactions between organisms in nature, and antimicrobial VOCs are promising, environmentally safer fumigants to control phytopathogens. As the mechanisms by which VOCs inhibit microorganisms are not well characterized, we evaluated the proteomic response in P. citricarpa after exposure for 12h to a reconstituted mixture of VOCs (alcohols and esters) originally identified in S...
February 2016: Microbiological Research
Kerly Laskoski, Adrian R S Santos, Ana C Bonatto, Fábio O Pedrosa, Emanuel M Souza, Luciano F Huergo
Nicotinamide adenine dinucleotide synthetase enzyme (NadE) catalyzes the amination of nicotinic acid adenine dinucleotide (NaAD) to form NAD(+). This reaction represents the last step in the majority of the NAD(+) biosynthetic routes described to date. NadE enzymes typically use either glutamine or ammonium as amine nitrogen donor, and the reaction is energetically driven by ATP hydrolysis. Given the key role of NAD(+) in bacterial metabolism, NadE has attracted considerable interest as a potential target for the development of novel antibiotics...
May 2016: Archives of Microbiology
Caroline Turchetto, Ana Lúcia A Segatto, Geraldo Mäder, Daniele M Rodrigues, Sandro L Bonatto, Loreta B Freitas
The analysis of genetic structure and variability of isolated species is of critical importance in evaluating whether stochastic or human-caused factors are affecting rare species. Low genetic diversity compromises the ability of populations to evolve and reduces their chances of survival under environmental changes. Petunia secreta, a rare and endemic species, is an annual and heliophilous herb that is bee-pollinated and easily recognizable by its purple and salverform corolla. It was described as a new species of the Petunia genus in 2005...
2016: AoB Plants
João Felipe Bonatto Bruniera, Yara Teresinha Corrêa Silva-Sousa, Paulo Esteves Pinto Faria
Oral rehabilitation with dental implants has become a routine treatment in contemporary dentistry. The displacement of dental implants into the sinus membrane, a complication related to the maxillary sinus, is one of the most common accidents reported in the literature. The treatment for this complication is the surgical removal of the implant. A 60-year-old woman with three dental implants displaced into the maxillary sinus (one implant displaced into the left maxillary sinus and two implants displaced into the right maxillary sinus) underwent surgery for removal of the implants...
2015: Case Reports in Dentistry
Katia C Oliveira, Mariana L P Carvalho, José Matheus C Bonatto, Debora Schechtman, Sergio Verjovski-Almeida
Schistosoma mansoni and its vertebrate host have a complex and intimate connection in which several molecular stimuli are exchanged and affect both organisms. Human tumor necrosis factor alpha (hTNF-α), a pro-inflammatory cytokine, is known to induce large-scale gene expression changes in the parasite and to affect several parasite biological processes such as metabolism, egg laying, and worm development. Until now, the molecular mechanisms for TNF-α activity in worms are not completely understood. Here, we aimed at exploring the effect of hTNF-α on S...
February 2016: Parasitology Research
Lucila Arantes Cecilio, Mauro W Bonatto
BACKGROUND: Celiac disease is an enteropathy characterized by gluten sensitivity and broad clinical aspect. Has a multifactorial cause and depends on genetic, immunological and environmental factors for its development. The genetic influence is given mostly by the human leukocyte antigens HLA DQ2 and DQ8. AIM: To evaluate the prevalence of human leukocyte antigens DQ2 and DQ8 in three different groups: patients with celiac disease, first-degree relatives and the general population...
July 2015: Arquivos Brasileiros de Cirurgia Digestiva: ABCD, Brazilian Archives of Digestive Surgery
Daniela Micheline Dos Santos, Adrielle Mendes De Paula, Liliane da Rocha Bonatto, Emily Vivianne Freitas Da Silva, Aljomar José Vechiato Filho, Amália Moreno, Marcelo Coelho Goiato
PURPOSE: To evaluate the influence of colorant solutions on color stability and surface roughness of indirect resin composites submitted to prior immersion in mouthwashes. METHODS: Five brands of indirect resin composites were assessed: Adoro, Resilab, Cristobal, Sinfony and Epricord. The specimens were immersed in five different solutions (n = 10): four mouthwashes (Listerine, Oral-B, Plax, Periogard) and artificial saliva (control). 60 hours after immersion in mouthwashes, the specimens were exposed to coffee solution...
August 2015: American Journal of Dentistry
Raquel Calloni, Diego Bonatto
Artificial miRNAs (amiRNAs) are molecules that have been developed to promote gene silencing in a similar manner to naturally occurring miRNAs. amiRNAs are generally constructed by replacing the mature miRNA sequence in the pre-miRNA stem-loop with a sequence targeting a gene of interest. These molecules offer an interesting alternative to silencing approaches that are based on shRNAs and siRNAs because they present the same efficiency as these options and are less cytotoxic. amiRNAs have mostly been applied to gene knockdown in plants; they have been examined to a lesser extent in animal cells...
October 2015: Human Gene Therapy Methods
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