keyword
MENU ▼
Read by QxMD icon Read
search

SMIFH2

keyword
https://www.readbyqxmd.com/read/26898799/smifh2-mediated-mdia-formin-functional-inhibition-potentiates-chemotherapeutic-targeting-of-human-ovarian-cancer-spheroids
#1
Megan A Ziske, Krista M Pettee, MaNada Khaing, Kaitlin Rubinic, Kathryn M Eisenmann
Due to a lack of effective screening or prevention protocol for epithelial ovarian cancer (EOC), there is a critical unmet need to develop therapeutic interventions for EOC treatment. EOC metastasis is unique. Initial dissemination is not primarily hematogenous, yet is facilitated through shedding of primary tumor cells into the peritoneal fluid and accumulating ascites. Increasingly, isolated patient spheroids point to a clinical role for spheroids in EOC metastasis. EOC spheroids are highly invasive structures that disseminate upon peritoneal mesothelium, and visceral tissues including liver and omentum...
March 25, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/26738547/arabidopsis-fh1-formin-affects-cotyledon-pavement-cell-shape-by-modulating-cytoskeleton-dynamics
#2
Amparo Rosero, Denisa Oulehlová, Lenka Stillerová, Petra Schiebertová, Michal Grunt, Viktor Žárský, Fatima Cvrčková
Plant cell morphogenesis involves concerted rearrangements of microtubules and actin microfilaments. We previously reported that FH1, the main Arabidopsis thaliana housekeeping Class I membrane-anchored formin, contributes to actin dynamics and microtubule stability in rhizodermis cells. Here we examine the effects of mutations affecting FH1 (At3g25500) on cell morphogenesis and above-ground organ development in seedlings, as well as on cytoskeletal organization and dynamics, using a combination of confocal and variable angle epifluorescence microscopy with a pharmacological approach...
March 2016: Plant & Cell Physiology
https://www.readbyqxmd.com/read/26609071/integration-of-linear-and-dendritic-actin-nucleation-in-nck-induced-actin-comets
#3
Sofya Borinskaya, Katrina B Velle, Kenneth G Campellone, Arthur Talman, Diego Alvarez, Hervé Agaisse, Yi I Wu, Leslie M Loew, Bruce J Mayer
The Nck adaptor protein recruits cytosolic effectors such as N-WASP that induce localized actin polymerization. Experimental aggregation of Nck SH3 domains at the membrane induces actin comet tails--dynamic, elongated filamentous actin structures similar to those that drive the movement of microbial pathogens such as vaccinia virus. Here we show that experimental manipulation of the balance between unbranched/branched nucleation altered the morphology and dynamics of Nck-induced actin comets. Inhibition of linear, formin-based nucleation with the small-molecule inhibitor SMIFH2 or overexpression of the formin FH1 domain resulted in formation of predominantly circular-shaped actin structures with low mobility (actin blobs)...
January 15, 2016: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/26574597/profilin-dependent-nucleation-and-assembly-of-actin-filaments-controls-cell-elongation-in-arabidopsis
#4
Lingyan Cao, Jessica L Henty-Ridilla, Laurent Blanchoin, Christopher J Staiger
Actin filaments in plant cells are incredibly dynamic; they undergo incessant remodeling and assembly or disassembly within seconds. These dynamic events are choreographed by a plethora of actin-binding proteins, but the exact mechanisms are poorly understood. Here, we dissect the contribution of Arabidopsis (Arabidopsis thaliana) PROFILIN1 (PRF1), a conserved actin monomer-binding protein, to actin organization and single filament dynamics during axial cell expansion of living epidermal cells. We found that reduced PRF1 levels enhanced cell and organ growth...
January 2016: Plant Physiology
https://www.readbyqxmd.com/read/26354425/small-molecule-agonists-of-mammalian-diaphanous-related-mdia-formins-reveal-an-effective-glioblastoma-anti-invasion-strategy
#5
Jessica D Arden, Kari I Lavik, Kaitlin A Rubinic, Nicolas Chiaia, Sadik A Khuder, Marthe J Howard, Andrea L Nestor-Kalinoski, Arthur S Alberts, Kathryn M Eisenmann
The extensive invasive capacity of glioblastoma (GBM) makes it resistant to surgery, radiotherapy, and chemotherapy and thus makes it lethal. In vivo, GBM invasion is mediated by Rho GTPases through unidentified downstream effectors. Mammalian Diaphanous (mDia) family formins are Rho-directed effectors that regulate the F-actin cytoskeleton to support tumor cell motility. Historically, anti-invasion strategies focused upon mDia inhibition, whereas activation remained unexplored. The recent development of small molecules directly inhibiting or activating mDia-driven F-actin assembly that supports motility allows for exploration of their role in GBM...
November 1, 2015: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/25970206/distinct-impact-of-targeted-actin-cytoskeleton-reorganization-on-mechanical-properties-of-normal-and-malignant-cells
#6
Yu M Efremov, A A Dokrunova, A V Efremenko, M P Kirpichnikov, K V Shaitan, O S Sokolova
The actin cytoskeleton is substantially modified in cancer cells because of changes in actin-binding protein abundance and functional activity. As a consequence, cancer cells have distinctive motility and mechanical properties, which are important for many processes, including invasion and metastasis. Here, we studied the effects of actin cytoskeleton alterations induced by specific nucleation inhibitors (SMIFH2, CK-666), cytochalasin D, Y-27632 and detachment from the surface by trypsinization on the mechanical properties of normal Vero and prostate cancer cell line DU145...
November 2015: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/25925024/smifh2-has-effects-on-formins-and-p53-that-perturb-the-cell-cytoskeleton
#7
Tadamoto Isogai, Rob van der Kammen, Metello Innocenti
Formin proteins are key regulators of the cytoskeleton involved in developmental and homeostatic programs, and human disease. For these reasons, small molecules interfering with Formins' activity have gained increasing attention. Among them, small molecule inhibitor of Formin Homology 2 domains (SMIFH2) is often used as a pharmacological Formin blocker. Although SMIFH2 inhibits actin polymerization by Formins and affects the actin cytoskeleton, its cellular mechanism of action and target specificity remain unclear...
2015: Scientific Reports
https://www.readbyqxmd.com/read/25837661/small-molecule-inhibitor-of-formin-homology-2-domains-smifh2-reveals-the-roles-of-the-formin-family-of-proteins-in-spindle-assembly-and-asymmetric-division-in-mouse-oocytes
#8
Hak-Cheol Kim, Yu-Jin Jo, Nam-Hyung Kim, Suk Namgoong
Dynamic actin reorganization is the main driving force for spindle migration and asymmetric cell division in mammalian oocytes. It has been reported that various actin nucleators including Formin-2 are involved in the polarization of the spindle and in asymmetric cell division. In mammals, the formin family is comprised of 15 proteins. However, their individual roles in spindle migration and/or asymmetric division have not been elucidated yet. In this study, we employed a newly developed inhibitor for formin family proteins, small molecule inhibitor of formin homology 2 domains (SMIFH2), to assess the functions of the formin family in mouse oocyte maturation...
2015: PloS One
https://www.readbyqxmd.com/read/23202131/atfh1-formin-mutation-affects-actin-filament-and-microtubule-dynamics-in-arabidopsis-thaliana
#9
Amparo Rosero, Viktor Žársky, Fatima Cvrčková
Plant cell growth and morphogenesis depend on remodelling of both actin and microtubule cytoskeletons. AtFH1 (At5g25500), the main housekeeping Arabidopsis formin, is targeted to membranes and known to nucleate and bundle actin. The effect of mutations in AtFH1 on root development and cytoskeletal dynamics was examined. Consistent with primarily actin-related formin function, fh1 mutants showed increased sensitivity to the actin polymerization inhibitor latrunculin B (LatB). LatB-treated mutants had thicker, shorter roots than wild-type plants...
January 2013: Journal of Experimental Botany
https://www.readbyqxmd.com/read/19942139/identification-and-characterization-of-a-small-molecule-inhibitor-of-formin-mediated-actin-assembly
#10
Syed A Rizvi, Erin M Neidt, Jiayue Cui, Zach Feiger, Colleen T Skau, Margaret L Gardel, Sergey A Kozmin, David R Kovar
Formins stimulate actin filament assembly for fundamental cellular processes including division, adhesion, establishing polarity, and motility. A formin inhibitor would be useful because most cells express multiple formins whose functions are not known and because metastatic tumor formation depends on the deregulation of formin-dependent processes. We identified a general small molecule inhibitor of formin homology 2 domains (SMIFH2) by screening compounds for the ability to prevent formin-mediated actin assembly in vitro...
November 25, 2009: Chemistry & Biology
1
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"