keyword
MENU ▼
Read by QxMD icon Read
search

itraq

keyword
https://www.readbyqxmd.com/read/28427148/in-depth-proteomic-profiling-of-left-ventricular-tissues-in-human-end-stage-dilated-cardiomyopathy
#1
Shanshan Liu, Yan Xia, Xiaohui Liu, Yi Wang, Zhangwei Chen, Juanjuan Xie, Juying Qian, Huali Shen, Pengyuan Yang
Dilated cardiomyopathy (DCM) is caused by reduced left ventricular (LV) myocardial function, which is one of the most common causes of heart failure (HF). We performed iTRAQ-coupled 2D-LC-MS/MS to profile the cardiac proteome of LV tissues from healthy controls and patients with end-stage DCM. We identified 4263 proteins, of which 125 were differentially expressed in DCM tissues compared to LV controls. The majority of these were membrane proteins related to cellular junctions and neuronal metabolism. In addition, these proteins were involved in membrane organization, mitochondrial organization, translation, protein transport, and cell death process...
February 25, 2017: Oncotarget
https://www.readbyqxmd.com/read/28423718/itraq-based-quantitative-proteomic-analysis-of-yamanaka-factors-reprogrammed-breast-cancer-cells
#2
Kun Wang, Zhiyan Shan, Lian Duan, Tiantian Gong, Feng Liu, Yue Zhang, Zhendong Wang, Jingling Shen, Lei Lei
Cancer cells had been developed to be reprogrammed into embryonic stem like cells by induced pluripotent stem cells (iPSCs) technology, however, the tumor differentiation/dedifferentiation mechanisms had not yet been analyzed on a genome-wide scale. Here, we inserted the four stem cell transcription factor genes OCT4, SOX2, C-MYC and KLF4 into MCF cells (MCFs), represented a female breast cancer cell type, and obtained iPSCs (Mcfips) in about 3 weeks. By using the LC MS/MS iTRAQ technology, we analyzed the proteomic changes between MCFs and Mcfips...
March 11, 2017: Oncotarget
https://www.readbyqxmd.com/read/28422320/mir-34b-modulates-skeletal-muscle-cell-proliferation-and-differentiation
#3
Zhixiong Tang, Huiling Qiu, Lan Luo, Nian Liu, Jiasheng Zhong, Kang Kang, Deming Gou
Myogenesis involves myoblast proliferation and differentiation to myocytes, followed by fusion and hypertrophy to form myotubes during muscle development. Increasing evidence showed that microRNAs (miRNAs) play important roles in the regulation of myogenesis. We have previously revealed that miR-34b is steadily increased during this process. This miRNA regulates differentiation in various cell types, though its function in myogenesis remains to be elucidated. In this study, we show that miR-34b represses muscle cell proliferation and promotes myotube formation...
April 19, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28422049/regulation-of-anthocyanin-biosynthesis-in-purple-leaves-of-zijuan-tea-camellia-sinensis-var-kitamura
#4
Lingxia Wang, Dezhuo Pan, Meng Liang, Yakubu Saddeeq Abubakar, Jian Li, Jinke Lin, Shipin Chen, Wei Chen
Plant anthocyanin biosynthesis is well understood, but the regulatory mechanism in purple foliage tea remains unclear. Using isobaric tag for relative and absolute quantification (iTRAQ), 815 differential proteins were identified in the leaves of Zijuan tea, among which 20 were associated with the regulation of anthocyanin metabolism. We found that the abundances of anthocyanin synthesis-related enzymes such as chalcone synthase, chalcone isomerase, dihydroflavonol 4-reductase and anthocyanin synthetase, as well as anthocyanin accumulation-related UDP-glucosyl transferase and ATP-binding cassette (ABC) transporters in the purple leaves were all significantly higher than those in the green leaves...
April 19, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28420861/potential-proteins-targeted-by-let-7f-5p-in-hela-cells
#5
Yu Wang, Xiujuan Chen, Yi Zhang, Jiandong Song
MicroRNAs are a class of small, endogenous, non-coding RNAs mediating posttranscriptional gene silencing. The current authors hypothesized that let-7f-5p is likely involved in cell invasion and proliferation by regulating the expression of target genes. The current study combined let-7f-5p with iTRAQ to assess its effect on gene expression in HeLa cells. Results indicated that 164 proteins were expressed at different levels in HeLa cells overexpressing let-7f-5p and negative controls and that 172 proteins were expressed at different levels in let-7f-5p-silenced HeLa cells and negative controls...
April 17, 2017: Bioscience Trends
https://www.readbyqxmd.com/read/28420359/saikosaponins-induced-hepatotoxicity-in-mice-via-lipid-metabolism-dysregulation-and-oxidative-stress-a-proteomic-study
#6
Xiaoyu Li, Xiaojiaoyang Li, Junxian Lu, Youyi Huang, Lili Lv, Yongfu Luan, Runping Liu, Rong Sun
BACKGROUND: Radix Bupleuri (RB) has been popularly used for treating many liver diseases such as chronic hepatic inflammation and viral Hepatitis in China. Increasing clinical and experimental evidence indicates the potential hepatotoxicity of RB or prescriptions containing RB. Recently, Saikosaponins (SS) have been identified as major bioactive compounds isolated from RB, which may be also responsible for RB-induced liver injury. METHODS: Serum AST, ALT and LDH levels were determined to evaluate SS-induced liver injury in mice...
April 19, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28419152/the-disadvantages-of-being-a-hybrid-during-drought-a-combined-analysis-of-plant-morphology-physiology-and-leaf-proteome-in-maize
#7
Dana Holá, Monika Benešová, Lukáš Fischer, Daniel Haisel, František Hnilička, Helena Hniličková, Petr L Jedelský, Marie Kočová, Dagmar Procházková, Olga Rothová, Lenka Tůmová, Naďa Wilhelmová
A comparative analysis of various parameters that characterize plant morphology, growth, water status, photosynthesis, cell damage, and antioxidative and osmoprotective systems together with an iTRAQ analysis of the leaf proteome was performed in two inbred lines of maize (Zea mays L.) differing in drought susceptibility and their reciprocal F1 hybrids. The aim of this study was to dissect the parent-hybrid relationships to better understand the mechanisms of the heterotic effect and its potential association with the stress response...
2017: PloS One
https://www.readbyqxmd.com/read/28418908/plasma-protein-profiling-in-patients-undergoing-coronary-artery-bypass-grafting-surgery-and-clinical-significance
#8
Zhi-Peng Guo, Hai-Tao Hou, Rui Jing, Zhen-Guo Song, Xiao-Cheng Liu, Guo-Wei He
This study was designed to identify the protein profiling in patients with triple vessel coronary artery disease (CAD) undergoing CABG, in order to detect CAD-related differential proteins in these patients. CABG patients with triple vessel disease with/without left main stenosis (n =160) were compared to normal coronary angiographic subjects (n =160). Plasma samples of 20 males and 20 females in each group were analyzed with iTRAQ technique. ELISA test was used to test the chosen proteins from iTRAQ results in plasma samples from a new cohort of the CABG group (n=120, male/femal=61/59) and control (n =120, male/female=60/60)...
March 18, 2017: Oncotarget
https://www.readbyqxmd.com/read/28418257/investigating-acquisition-performance-on-the-orbitrap-fusion-when-using-tandem-ms-ms-ms-scanning-with-isobaric-tags
#9
Christopher S Hughes, Victor Spicer, Oleg V Krokhin, Gregg B Morin
Methods for isobaric-tagged peptide analysis (e.g., TMT, iTRAQ), such as the synchronous precursor selection (SPS) tandem MS/MS/MS (MS(3)) approach, enable maintenance of reporter ion accuracy and precision by reducing the ratio compression caused by coisolated precursor ions. However, the decreased throughput of the MS(3) approach necessitates careful optimization of acquisition strategies and methods to ensure maximal proteome coverage. We present a systematic analysis of acquisition parameters used to analyze isobaric-tagged peptide samples on current generation Orbitrap mass spectrometer (MS) hardware...
April 20, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28418254/evaluating-the-characteristics-of-reporter-ion-signal-acquired-in-the-orbitrap-analyzer-for-isobaric-mass-tag-proteome-quantification-experiments
#10
Christopher S Hughes, Chenchen Zhu, Victor Spicer, Oleg V Krokhin, Gregg B Morin
Multiplexed quantification with isobaric chemical tags (e.g., TMT, iTRAQ) provides a robust and efficient means to comparatively examine proteome dynamics between several biological states using a mass spectrometer (MS). The quantitative nature of isobaric tags necessitates strict validation of the observed ion signals in the chosen MS detector before differential patterns are extracted between biological states. We present an in-depth analysis of isobaric tag data acquired on current generation Orbitrap MS hardware to illustrate pitfalls in acquisition settings that can negatively impact results...
April 20, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28408942/identification-of-tengfu-jiangya-tablet-target-biomarkers-with-quantitative-proteomic-technique
#11
Jingwen Xu, Yunlun Li, Shijun Zhang, Haiqiang Jiang, Nan Wang, Haiqing Lin
Tengfu Jiangya Tablet (TJT) is a well accepted antihypertension drug in China and its major active components were Uncaria total alkaloids and Semen Raphani soluble alkaloid. To further explore treatment effects mechanism of TJT on essential hypertension, a serum proteomic study was performed. Potential biomarkers were quantified in serum of hypertension individuals before and after taking TJT with isobaric tags for relative and absolute quantitation (iTRAQ) coupled two-dimensional liquid chromatography followed electrospray ionization-tandem mass spectrometry (2D LC-MS/MS) proteomics technique...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/28406696/proteomics-approach-for-identification-of-iga-nephropathy-related-biomarkers-in-urine
#12
P Prikryl, L Vojtova, D Maixnerova, M Vokurka, M Neprasova, T Zima, V Tesar
Proteinuria is often used as a surrogate marker in monitoring and predicting outcome in patients with chronic kidney diseases, but it is non-specific. IgAN belongs to the most common primary glomerulonephritis worldwide with serious prognosis. The main aim of this work was to assess differences in urine proteins in patients with IgA nephropathy and to identify abnormal proteins as potential biomarkers of IgA nephropathy or the renal disease. In our pilot project, we selected 20 patients and compared them with 20 healthy volunteers...
April 12, 2017: Physiological Research
https://www.readbyqxmd.com/read/28404849/quantitative-proteomic-analysis-of-mosquito-c6-36-cells-reveals-host-proteins-involved-in-zika-virus-infection
#13
Qi-Lin Xin, Cheng-Lin Deng, Xi Chen, Jun Wang, Shao-Bo Wang, Wei Wang, Fei Deng, Bo Zhang, Gengfu Xiao, Lei-Ke Zhang
Zika virus (ZIKV) is an emerging arbovirus belonging to the genus Flavivirus of the family Flaviviridae During replication processes, flavivirus will manipulate host cell systems to facilitate their replication, while the host cells will activate antiviral responses. Identification of host proteins involved in flavivirus replication process may lead to the discovery of antiviral targets. Mosquito Aedes aegypti and Aedes albopictus are epidemiologically important vectors for ZIKV, and effective restrictions of ZIKV replication in mosquitoes will be vital in controlling the spread of virus...
April 12, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28398533/quantitative-proteomics-analysis-of-developmental-reprogramming-in-protoplast-of-the-moss-physcomitrella-patens
#14
Xiaoqin Wang, Lu Chen, Aizhen Yang, Chunya Bu, Yikun He
The moss Physcomitrella patens is a model system for studying developmental plant processes. To better understand the biochemical and physiological changes involved in developmental reprogramming, we conducted a quantitative proteomics analysis for protonemata, protoplasts made therefrom and protoplasts regenerated for two days. Using an iTRAQ peptide labelling strategy and liquid chromatography-tandem mass spectrometry (LC-MS/MS), more than 3000 peptides and 1000 proteins were quantified. Of these, 162 proteins were identified as having differential abundances during developmental reprogramming...
April 8, 2017: Plant & Cell Physiology
https://www.readbyqxmd.com/read/28396508/adipocyte-metabolic-pathways-regulated-by-diet-control-the-female-germline-stem-cell-lineage-in-drosophila
#15
Shinya Matsuoka, Alissa Armstrong, Leesa L Sampson, Kaitlin M Laws, Daniela Drummond-Barbosa
Nutrients affect adult stem cells through complex mechanisms involving multiple organs. Adipocytes are highly sensitive to diet and have key metabolic roles, and obesity increases the risk for many cancers. How diet-regulated adipocyte metabolic pathways influence normal stem cell lineages, however, remains unclear. Drosophila melanogaster has highly conserved adipocyte metabolism and a well-characterized female germline stem cell (GSC) lineage response to diet. Here, we conducted an isobaric tags for relative and absolute quantification (iTRAQ) proteomic analysis to identify diet-regulated adipocyte metabolic pathways that control the female GSC lineage...
April 10, 2017: Genetics
https://www.readbyqxmd.com/read/28391008/recent-5-year-findings-and-technological-advances-in-the-proteomic-study-of-hiv-associated-disorders
#16
REVIEW
Lijun Zhang, Xiaofang Jia, Jun-O Jin, Hongzhou Lu, Zhimi Tan
Human immunodeficiency virus-1 (HIV-1) mainly relies on host factors to complete its life cycle. Hence, it is very important to identify HIV-regulated host proteins. Proteomics is an excellent technique for this purpose because of its high throughput and sensitivity. In this review, we summarized current technological advances in proteomics, including general isobaric tags for relative and absolute quantitation (iTRAQ) and stable isotope labeling by amino acids in cell culture (SILAC), as well as subcellular proteomics and investigation of posttranslational modifications...
April 5, 2017: Genomics, Proteomics & Bioinformatics
https://www.readbyqxmd.com/read/28390150/quantitative-proteomics-investigation-of-leaves-from-two-sedum-alfredii-crassulaceae-populations-that-differ-in-cadmium-accumulation
#17
Zhongchun Zhang, Huina Zhou, Qi Yu, Yunxia Li, David G Mendoza-Cózatl, Baosheng Qiu, Pingping Liu, Qiansi Chen
Due to extraordinary their capacity to hypertolerate and hyperaccumulate heavy metals in above-ground tissues, hyperaccumulator species have gained wide attention from researchers seeking biotechnologies to manage environmental heavy metal pollution. However, the molecular basis of hyperaccumulation is still far from being fully understood. Here, we used iTRAQ to perform a quantitative proteomics study of the leaves of Sedum alfredii (Crassulaceae) from hyperaccumulating (HP) and non-hyperaccumulating (NHP) populations...
April 8, 2017: Proteomics
https://www.readbyqxmd.com/read/28388555/targeting-mantle-cell-lymphoma-metabolism-and-survival-through-simultaneous-blockade-of-mtor-and-nuclear-transporter-exportin-1
#18
Kazumasa Sekihara, Kaori Saitoh, Lina Han, Stefan Ciurea, Shinichi Yamamoto, Mika Kikkawa, Saiko Kazuno, Hikari Taka, Naoko Kaga, Hajime Arai, Takashi Miida, Michael Andreeff, Marina Konopleva, Yoko Tabe
Mantle cell lymphoma (MCL) is an aggressive B-cell lymphoma with poor prognosis, characterized by aberrant expression of growth-regulating and oncogenic effectors and requiring novel anticancer strategies. The nuclear transporter exportin-1 (XPO1) is highly expressed in MCL and is associated with its pathogenesis. mTOR signaling, a central regulator of cell metabolism, is frequently activated in MCL and is also an important therapeutic target in this cancer. This study investigated the antitumor effects and molecular/metabolic changes induced by the combination of the small-molecule selective inhibitor XPO1 inhibitor KPT-185 and the dual mTORC1/2 kinase inhibitor AZD-2014 on MCL cells...
March 27, 2017: Oncotarget
https://www.readbyqxmd.com/read/28387650/a-proteomic-analysis-of-the-malignant-mesothelioma-secretome-using-itraq
#19
Jenette Creaney, Ian M Dick, Justine S Leon, Bruce W S Robinson
Backgound/Aim: Malignant mesothelioma (MM) is an aggressive and fatal pleural cancer. The cell secretome offers information allowing insight into the pathogenesis of MM while offering the possibility to identify potential therapeutic targets and biomarkers. In the present study the secretome protein profile of MM cell lines was compared to normal mesothelial cells. MATERIALS AND METHODS: Six MM cell lines were compared against three primary mesothelial cell culture preparations using iTRAQ® mass spectrometry...
March 2017: Cancer Genomics & Proteomics
https://www.readbyqxmd.com/read/28378827/real-time-itraq-based-proteome-profiling-revealed-the-central-metabolism-involved-in-nitrogen-starvation-induced-lipid-accumulation-in-microalgae
#20
Vineeta Rai, Muthusivaramapandian Muthuraj, Mayuri N Gandhi, Debasish Das, Sanjeeva Srivastava
To understand the post-transcriptional molecular mechanisms attributing to oleaginousness in microalgae challenged with nitrogen starvation (N-starvation), the longitudinal proteome dynamics of Chlorella sp. FC2 IITG was investigated using multipronged quantitative proteomics and multiple reaction monitoring assays. Physiological data suggested a remarkably enhanced lipid accumulation with concomitant reduction in carbon flux towards carbohydrate, protein and chlorophyll biosynthesis. The proteomics-based investigations identified the down-regulation of enzymes involved in chlorophyll biosynthesis (porphobilinogen deaminase) and photosynthetic carbon fixation (sedoheptulose-1,7 bisphosphate and phosphoribulokinase)...
April 5, 2017: Scientific Reports
keyword
keyword
106765
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"