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https://www.readbyqxmd.com/read/28813468/proteomic-comparison-by-itraq-combined-with-mass-spectrometry-of-egg-white-proteins-in-laying-hens-gallus-gallus-fed-with-soybean-meal-and-cottonseed-meal
#1
Tao He, Haijun Zhang, Jing Wang, Shugeng Wu, Hongyuan Yue, Guanghai Qi
Cottonseed meal (CSM) is commonly used in hens' diets to replace soybean meal (SBM). However, the molecular consequences of this substitution remains unclear. To investigate the impact of this substitution at the molecular level, iTRAQ combined with biochemical analysis was performed in Hy-Line W-36 hens supplemented with a mixed diet of CSM and SBM. Egg weight, albumen height, and Haugh unit were significantly reduced in the CSM100 group (100% crude protein of SBM replaced by CSM) compared with the SBM group (P<0...
2017: PloS One
https://www.readbyqxmd.com/read/28806774/fibroblast-growth-factor-receptor-1-mediates-internalization-of-pathogenic-spotted-fever-rickettsiae-into-host-endothelium
#2
Abha Sahni, Jignesh Patel, Hema P Narra, Casey L C Schroeder, David H Walker, Sanjeev K Sahni
Rickettsial infections continue to cause serious morbidity and mortality in severe human cases around the world. Host cell adhesion and invasion is an essential requisite for intracellular growth, replication, and subsequent dissemination of pathogenic rickettsiae. Heparan sulfate proteoglycans [HSPGs] facilitate the interactions between fibroblast growth factor(s) and their tyrosine kinase receptors resulting in receptor dimerization/activation and have been implicated in bacterial adhesion to target host cells...
2017: PloS One
https://www.readbyqxmd.com/read/28804543/light-of-dna-alkylating-agents-in-castration-resistant-prostate-cancer-cells-a-novel-mixed-egfr-dna-targeting-combi-molecule
#3
Guan-Can Liang, Hao-Feng Zheng, Yan-Xiong Chen, Teng-Cheng Li, Wei Liu, You-Qiang Fang
OBJECTIVE: The mechanism underlying the therapeutic effects of combi-molecule JDF12 on prostate cancer (PCa) DU145 cells remains still unclear. This study aimed to investigate the proteomic profile after JDF12 treatment in DU145 cells by comparing with that in Iressa treated cells and untreated cells. METHODS: MTT was used to evaluate drug cytotoxicity, DAPI staining was done to assess apoptosis of cells, and flow cytometry was used to analyze cell cycle. iTRAQ and qPCR were employed to obtain the proteomic profiles of JDF12 treated, Iressa treated, and untreated DU145 cells, and validate the expression of selected differentially expressed proteins, respectively...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28800743/an-itraq-based-proteomic-analysis-reveals-dysregulation-of-neocortical-synaptopodin-in-lewy-body-dementias
#4
Arnab Datta, Yuek Ling Chai, Jing Min Tan, Jasinda H Lee, Paul T Francis, Christopher P Chen, Siu Kwan Sze, Mitchell K P Lai
Lewy body dementias are the second most common cause of neurodegenerative dementia in the elderly after Alzheimer's disease (AD). The two clinical subgroups of Lewy body dementias, namely, dementia with Lewy bodies (DLB) and Parkinson's disease dementia (PDD), are differentiated by the chronology of cognitive symptoms relative to parkinsonism. At present, there remains a debate on whether DLB and PDD are separate disease entities, or fall within the same spectrum of Lewy body dementias. In this study, we compared the detergent-soluble proteome via an 8-plex isobaric tag for relative and absolute quantitation (iTRAQ) analysis of pooled lysates from the prefrontal cortex (BA9) of DLB (n = 19) and PDD (n = 21) patients matched a priori for amyloid (total Aβ42) burden, semi-quantitative scores for Lewy bodies and neurofibrillary tangles together with age-matched control (n = 21) subjects...
August 11, 2017: Molecular Brain
https://www.readbyqxmd.com/read/28800123/differential-proteomic-analysis-reveals-the-effect-of-calcium-on-malus-baccata-borkh-leaves-under-temperature-stress
#5
Lijie Li, Hong Su, Huaiyu Ma, Deguo Lyu
In the cool apple-producing areas of northern China, air temperature during early spring changes in a rapid and dramatic manner, which affects the growth and development of apple trees at the early stage of the growing season. Previous studies have shown that the treatment of calcium can increase the cold tolerance of Malus baccata Borkh., a widely-used rootstock apple tree in northern China. To better understand the physiological function of calcium in the response of M. baccata to temperature stress, we analyzed the effect of calcium treatment (2% CaCl₂) on M...
August 11, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28790852/impact-of-combined-treatment-with-nimesulide-and-cisplatin-on-oral-carcinoma-cells
#6
Aleksandra Barac, Goran Mitulovic, Seth Hallström, Sonja Zehetmayer, Matthaeus Ch Grasl, Boban M Erovic
BACKGROUND: Despite significant advances in diagnosis and therapy, the rate of survival of patients with oral cancers still remains poor as an appropriate treatment has not been found yet, due to side effects of chemo/radiotherapy. AIM: This study aimed to identify molecular mechanisms of cell death of oral cancer cells caused by treatment with a nonselective Cox-2 inhibitor in combination with a low-dose chemotherapeutic drug. METHODS: Squamous cell carcinoma (SCC) cells SCC9 and SCC25 were subjected to mono- and combination therapy with nimesulide and cisplatin...
2017: OncoTargets and Therapy
https://www.readbyqxmd.com/read/28790462/itraq-and-virus-induced-gene-silencing-revealed-three-proteins-involved-in-cold-response-in-bread-wheat
#7
Ning Zhang, Lingran Zhang, Lei Zhao, Yan Ren, Dangqun Cui, Jianhui Chen, Yongyan Wang, Pengbo Yu, Feng Chen
By comparing the differentially accumulated proteins from the derivatives (UC 1110 × PI 610750) in the F10 recombinant inbred line population which differed in cold-tolerance, altogether 223 proteins with significantly altered abundance were identified. The comparison of 10 cold-sensitive descendant lines with 10 cold-tolerant descendant lines identified 140 proteins that showed decreased protein abundance, such as the components of the photosynthesis apparatus and cell-wall metabolism. The identified proteins were classified into the following main groups: protein metabolism, stress/defense, carbohydrate metabolism, lipid metabolism, sulfur metabolism, nitrogen metabolism, RNA metabolism, energy production, cell-wall metabolism, membrane and transportation, and signal transduction...
August 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28780297/respiration-disruption-and-detoxification-at-the-protein-expression-levels-in-the-pacific-oyster-crassostrea-gigas-under-zinc-exposure
#8
Jie Meng, Wen-Xiong Wang, Li Li, Guofan Zhang
The Pacific oyster (Crassostrea gigas) can accumulate high levels of zinc (Zn) without obvious toxicity, but the related molecular mechanisms are largely unknown. In the present study, C. gigas were exposed to excess Zn for 9days and the differentially expressed proteins (DEPs) were examined using the isobaric tags for relative and absolute quantitation (iTRAQ) method. In total, 2667 proteins containing at least two peptides and detected in both replicates were used for proteomic analysis. Among these DEPs, 332 were up-regulated and 282 were down-regulated...
July 26, 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/28771929/comparative-itraq-based-quantitative-proteomic-analysis-of-pelteobagrus-vachelli-liver-against-acute-hypoxia-implications-in-metabolic-responses
#9
Guosong Zhang, Jiajia Zhang, Xin Wen, Cheng Zhao, Hongye Zhang, Xinru Li, Shaowu Yin
More and more frequently these days, aquatic ecosystems are being stressed by nutrient enrichment, pollutants, and global warming, leading to a serious depletion in oxygen concentrations. Although a sudden, significant lack of oxygen will result in mortality, fishes can have an acute behaviour (e.g., an increase in breathing rate, reduction in swimming frequency) and physiology responses (e.g., increase in oxygen delivery, and reduction in oxygen consumption) to hypoxia, which allows them to maintain normal physical activity...
August 3, 2017: Proteomics
https://www.readbyqxmd.com/read/28769938/unraveling-the-root-proteome-changes-and-its-relationship-to-molecular-mechanism-underlying-salt-stress-response-in-radish-raphanus-sativus-l
#10
Xiaochuan Sun, Yan Wang, Liang Xu, Chao Li, Wei Zhang, Xiaobo Luo, Haiyan Jiang, Liwang Liu
To understand the molecular mechanism underlying salt stress response in radish, iTRAQ-based proteomic analysis was conducted to investigate the differences in protein species abundance under different salt treatments. In total, 851, 706, and 685 differential abundance protein species (DAPS) were identified between CK vs. Na100, CK vs. Na200, and Na100 vs. Na200, respectively. Functional annotation analysis revealed that salt stress elicited complex proteomic alterations in radish roots involved in carbohydrate and energy metabolism, protein metabolism, signal transduction, transcription regulation, stress and defense and transport...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28763705/proteomic-analysis-of-broccoli-sprouts-by-itraq-in-response-to-jasmonic-acid
#11
Liping Guo, Pei Wang, Zhenxin Gu, Xiaolin Jin, Runqiang Yang
Jasmonic acid (JA) is well known as a linolenic acid-derived signal molecule related to the plant response to biotic and abiotic stresses. JA can regulate various plant metabolisms, such as glucosinolate metabolism. In this study, the proteome profiles of broccoli sprouts under JA treatment were analyzed using the iTRAQ-based quantitative proteome approach. A total of 122 differentially expressed proteins participating in a wide range of physiological processes were confidently identified in broccoli sprouts treated with JA...
July 11, 2017: Journal of Plant Physiology
https://www.readbyqxmd.com/read/28755911/itraq-proteomic-analysis-of-the-interactions-between-bombyx-mori-nuclear-polyhedrosis-virus-and-silkworm
#12
Wei-Tao Dong, Long-Fei Xiao, Jun-Jie Hu, Xin-Xu Zhao, Ji-Xing Liu, Yong Zhang
The silkworm hemolymph is an important defense system against bacteria and viruses. In this study, silkworms were infected with Bombyx mori nuclear polyhedrosis virus to investigate the subsequent immune response at the protein level. Proteomes were analyzed before and after infection using isobaric tags for relative and absolute quantitation and LC-MS. A total of 456 differentially expressed proteins were identified, of which 179 were upregulated and 193 were downregulated. Changes in expression were validated by western blot for several proteins...
July 26, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28752295/nmr-based-metabolomics-reveals-the-metabolite-profiles-of-vibrio-parahaemolyticus-under-ferric-iron-stimulation
#13
Jun Zhou, Chenyang Lu, Dijun Zhang, Chennv Ma, Xiurong Su
Vibrio parahaemolyticus is a halophilic bacterium endemic to coastal areas, and its pathogenicity has caused widespread seafood poisoning. In our previous research, the protein expression of V. parahaemolyticus in Fe(3+) medium was determined using isobaric tags for relative and absolute quantitation (iTRAQ). Here, nuclear magnetic resonance (NMR) was used to detect changes in the V. parahaemolyticus metabolome. NMR spectra were obtained using methanol-water extracts of intracellular metabolites from V. parahaemolyticus under various culture conditions, and 62 metabolites were identified, including serine, arginine, alanine, ornithine, tryptophan, glutamine, malate, NAD(+), NADP(+), oxypurinol, xanthosine, dCTP, uracil, thymine, hypoxanthine, and betaine...
August 2017: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/28752099/expressions-profiles-of-the-proteins-associated-with-carbohydrate-metabolism-in-rat-liver-regeneration
#14
Li Yin, Cuifang Chang, Cunshuan Xu
Liver has a very amazing ability to regenerate from the remnant liver after injury or partial hepatectomy (PH). Carbohydrate metabolism plays a critical role in regeneration. Many signaling pathways are involved in the metabolism process. We analyzed the changes of proteins at 0-36 h after PH in rats using isobaric tags for relative and absolute quantitation (iTRAQ) coupled with LC-MS/MS-based quantitative proteomics strategy. The results showed that 110 proteins and 5 signaling pathways related to carbohydrate metabolism in rat LR changed significantly...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28750031/dissecting-the-proteome-dynamics-of-the-salt-stress-induced-changes-in-the-leaf-of-diploid-and-autotetraploid-paulownia-fortunei
#15
Minjie Deng, Yanpeng Dong, Zhenli Zhao, Yongsheng Li, Guoqiang Fan
Exposure to high salinity can trigger acclimation in many plants. Such an adaptative response is greatly advantageous for plants and involves extensive reprogramming at the molecular level. Acclimation allows plants to survive in environments that are prone to increasing salinity. In this study, diploid and autotetraploid Paulownia fortunei seedlings were used to detect alterations in leaf proteins in plants under salt stress. Up to 152 differentially abundant proteins were identified by Multiplex run iTRAQ-based quantitative proteomic and LC-MS/MS methods...
2017: PloS One
https://www.readbyqxmd.com/read/28749931/quantitative-multiplexed-workflow-for-deep-analysis-of-human-blood-plasma-and-biomarker-discovery-by-mass-spectrometry
#16
Hasmik Keshishian, Michael W Burgess, Harrison Specht, Luke Wallace, Karl R Clauser, Michael A Gillette, Steven A Carr
Proteomic characterization of blood plasma is of central importance to clinical proteomics and particularly to biomarker discovery studies. The vast dynamic range and high complexity of the plasma proteome have, however, proven to be serious challenges and have often led to unacceptable tradeoffs between depth of coverage and sample throughput. We present an optimized sample-processing pipeline for analysis of the human plasma proteome that provides greatly increased depth of detection, improved quantitative precision and much higher sample analysis throughput as compared with prior methods...
August 2017: Nature Protocols
https://www.readbyqxmd.com/read/28745444/comparison-of-the-proteomes-of-mouse-skin-derived-precursors-skps-and-skp-derived-fibroblasts-sfbs-by-itraq
#17
Yujie Mao, Lidan Xiong, Li Li
Skin-derived precursors (SKPs) can differentiate into fibroblasts. However, little is known about the molecular mechanism of the differentiation process. Our previous study has compared the transcriptomes of mouse SKPs and SKP- derived fibroblasts (SFBs) by RNA-Seq analysis and found some genes and signal pathways that might play important roles in the transition from SKPs to SFBs. Here, to further investigate the process by which SKPs differentiate into fibroblasts, we compared the proteomes between SKPs and SFBs using iTRAQ...
July 26, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28736523/sub-minimum-inhibitory-concentrations-of-rhubarb-water-extracts-inhibit-streptococcus-suis-biofilm-formation
#18
Wen-Ya Ding, Yan-Hua Li, He Lian, Xiao-Yu Ai, Yu-Lin Zhao, Yan-Bei Yang, Qiang Han, Xin Liu, Xue-Ying Chen, Zhonggui He
Streptococcus suis is one of the most important swine pathogens, which can cause persistent infection by forming biofilms. In this study, sub-minimum inhibitory concentration (sub-MIC) of rhubarb water extracts were found to inhibit biofilm formation. Two-component signal transduction systems (TCSs), transcriptional regulators, and DNA binding proteins were compared under two conditions: (1) cells treated with sub-MIC rhubarb water extracts and (2) untreated cells. Using an isobaric tags for relative and absolute quantitation (iTRAQ) strategy, we found that TCSs constituent proteins of histidine kinase and response regulator were significantly down-regulated...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28736360/-s100a4-a-potential-therapeutic-target-on-bladder-cancer-stem-cells
#19
Chun-Yan Wang, Qing-Wen Nie, Xuan Zhou, Da-Xiong Huang, Wei-Qiang Xiao, Yong-Tong Zhu
OBJECTIVE: To observe the effect of S100A4 gene silencing mediated by small interfering RNA (siRNA) on the proliferation of bladder cancer stem cells (CSCs) and their capacity of xenograft tumor formation. METHODS: MB49 bladder cancer stem cells (MCSCs) were isolated and identified. The differentially expressed protein S100A4 was identified in MCSCs using isobaric tags for relative and absolute quantitation technology (iTRAQ). A siRNA targeting S100A4 was constructed and transfected into MCSCs, and its inhibitory effects on S100A4 expression in MCSCs were assessed with Western blotting and qPCR...
July 20, 2017: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/28732011/proteomics-analysis-reveals-a-dynamic-diurnal-pattern-of-photosynthesis-related-pathways-in-maize-leaves
#20
Dan Feng, Yanwei Wang, Tiegang Lu, Zhiguo Zhang, Xiao Han
Plant leaves exhibit differentiated patterns of photosynthesis rates under diurnal light regulation. Maize leaves show a single-peak pattern without photoinhibition at midday when the light intensity is maximized. This mechanism contributes to highly efficient photosynthesis in maize leaves. To understand the molecular basis of this process, an isobaric tag for relative and absolute quantitation (iTRAQ)-based proteomics analysis was performed to reveal the dynamic pattern of proteins related to photosynthetic reactions...
2017: PloS One
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