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https://www.readbyqxmd.com/read/29778923/quantitative-proteomic-profiling-indicates-the-difference-in-reproductive-efficiency-between-meishan-and-duroc-boar-spermatozoa
#1
Li Xinhong, Linqing Zhen, Jieli Fu, Lirui Wang, Qiangzhen Yang, Peifei Li, Yuhua Li
The reproductive efficiency of Meishan pigs is higher than that of Duroc pigs, but the underlying molecular mechanism for this disparity remains unclear. No systematic quantitative proteomics studies, comparing global proteins in Meishan and Duroc boar spermatozoa have been reported. Therefore, we applied iTRAQ labeling coupled with mass spectrometry, and analyzed the differences in proteins between Meishan and Duroc sperm. In the present study, a total of 1597 proteins were quantified. Of these proteins, 190 showed statistically significant fold changes between Meishan and Duroc spermatozoa...
April 30, 2018: Theriogenology
https://www.readbyqxmd.com/read/29777486/physiological-and-quantitative-proteomic-analyses-unraveling-potassium-deficiency-stress-response-in-alligator-weed-alternanthera-philoxeroides-l-root
#2
Li-Qin Li, Lun Liu, Wei Zhuo, Qian Chen, Sheng Hu, Shuang Peng, Xi-Yao Wang, Yi-Fei Lu, Li-Ming Lu
Physiological and iTRAQ based proteomic analysis provided new insights into potassium deficiency stress response in alligator weed root. Alligator weed (Alternanthera philoxeroides) has a strong ability to adapt to potassium deficiency (LK) stress. Proteomic changes in response to this stress are largely unknown in alligator weed. In this study, we investigated physiological and molecular mechanisms under LK using isobaric tags for relative and absolute quantitation to characterize proteome-level changes in this plant...
May 18, 2018: Plant Molecular Biology
https://www.readbyqxmd.com/read/29774118/proteomic-profiling-of-skeletal-and-cardiac-muscle-in-cancer-cachexia-alterations-in-sarcomeric-and-mitochondrial-protein-expression
#3
Angie M Y Shum, Anne Poljak, Nicholas L Bentley, Nigel Turner, Timothy C Tan, Patsie Polly
Background: Cancer cachexia is observed in more than 50% of advanced cancer patients, and impairs quality of life and prognosis. A variety of pathways are likely to be dysregulated. Hence, a broad-spectrum understanding of the disease process is best achieved by a discovery based approach such as proteomics. Results: More than 300 proteins were identified with > 95% confidence in correct sequence identification, of which 5-10% were significantly differentially expressed in cachectic tissues ( p -value of 0...
April 24, 2018: Oncotarget
https://www.readbyqxmd.com/read/29768655/let-7e-inhibits-tnf-%C3%AE-expression-by-targeting-the-methyl-transferase-ezh2-in-denv2-infected-thp-1-cells
#4
Yingke Zhang, Qianqian Zhang, Lian Gui, Yan Cai, Xiaohong Deng, Cheukfai Li, Qi Guo, Xiaoshun He, Junqi Huang
Tumor necrosis factor α (TNFα), an important inflammatory cytokine, is associated with dengue hemorrhagic fever/dengue shock syndrome (DHF/DSS), a severe pathological manifestation of dengue virus (DENV) infection. However, the regulatory mechanism of microRNA on TNFα is currently unknown. Our study showed that the TNFα expression increased immediately and then later decreased, while a marked increase for the miRNA let-7e was detected in dengue virus type 2 (DENV2)-infected peripheral blood mononuclear cells (PBMCs)...
May 16, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29761697/identification-of-proteins-using-itraq-and-vigs-reveals-three-bread-wheat-proteins-involved-in-the-response-to-combined-osmotic-cold-stress
#5
Ning Zhang, Lingran Zhang, Chaonan Shi, Lei Zhao, Dangqun Cui, Feng Chen
Crops are often subjected to a combination of stresses in the field. To date, studies on the physiological and molecular responses of common wheat to a combination of osmotic and cold stresses, however, remain unknown. In this study, wheat seedlings exposed to osmotic-cold stress for 24 h showed inhibited growth, as well as increased lipid peroxidation, relative electrolyte leakage, and soluble sugar contents. iTRAQ-based quantitative proteomics and liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods were employed to determine the proteomic profiles of the roots and leaves of wheat seedlings exposed to osmotic-cold stress conditions...
May 15, 2018: Journal of Proteome Research
https://www.readbyqxmd.com/read/29760307/identification-of-urinary-biomarkers-for-the-prediction-of-gestational-diabetes-mellitus-in-early-second-trimester-of-young-gravidae-based-on-itraq-quantitative-proteomics
#6
Ying Guo, Zhonghou Han, Li Guo, Yu Liu, Gang Li, Huiqing Li, Jie Zhang, Liwei Bai, Hongli Wu, Biliang Chen
Gestational Diabetes Mellitus (GDM) has brought great harm to maternal and fetus. Up to now, only a few plasma biomarkers for its early diagnosis have been reported; nevertheless, there is no report about identification of urinary biomarkers for prediction of GDM. Thus, it is necessary to correct this deficiency. In our study, urine samples were collected from 889 healthy young gravidae at the early second trimester (15 to 20 weeks), 69 of whom were subsequently diagnosed with GDM at 24 to 28 weeks. iTRAQ (the isobaric tags for relative and absolute quantification) quantitative proteomics was conducted on sixteen GDM (trial group) and an equal number of matched healthy young gravidae (control group)...
May 12, 2018: Endocrine Journal
https://www.readbyqxmd.com/read/29753025/identification-and-efficacy-of-glycine-serine-and-threonine-metabolism-in-potentiating-kanamycin-mediated-killing-of-edwardsiella-piscicida
#7
Jin-Zhou Ye, Xiang-Min Lin, Zhi-Xue Cheng, Yu-Bin Su, Wan-Xin Li, Far-Man Ali, Jun Zheng, Bo Peng
We previously showed that glucose potentiated kanamycin to kill multidrug-resistant Edwardsiella piscicida through activation of the TCA cycle. However, whether other regulatory mechanism is involved requires further investigation. By quantitative proteomics technology, iTRAQ, we systematically mapped the altered proteins in the presence of glucose and identified 94 differentially expressed proteins. The analysis of the altered proteins by pathways, amino acid biosynthesis and metabolism were enriched. And the most significantly altered eight amino acids tyrosine, phenylalanine, valine, leucine, isoleucine, glycine, serine and threonine were investigated for their potentiation of kanamycin to kill EIB202, where glycine, serine and threonine showed the strongest efficacy than the others...
May 9, 2018: Journal of Proteomics
https://www.readbyqxmd.com/read/29750532/proteomic-analysis-and-nir-ii-imaging-of-mcm2-protein-in-hepatocellular-carcinoma
#8
Jing Yang, Qi Xie, Hui Zhou, Lei Chang, Wei Wei, Yin Wang, Hong Li, Zixin Deng, Yuling Xiao, Junzhu Wu, Ping Xu, Xuechuan Hong
Targeted therapy of hepatocellular carcinoma (HCC) is essential for improved therapies. Therefore, identification of key targets specifically to HCC is an urgent requirement. Herein, an iTRAQ quantitative proteomic approach was employed to identify differentially expressed proteins in HCC tumor tissues. Of the upregulated tumor related proteins, minichromosome maintenance 2 (MCM2), a DNA replication licensing factor was one of the most significantly altered proteins, and its overexpression was confirmed using tissue microarray...
May 11, 2018: Journal of Proteome Research
https://www.readbyqxmd.com/read/29750162/screening-of-serum-protein-markers-for-avascular-osteonecrosis-of-femoral-head-differentially-expressed-after-treatment-with-yuanshi-shengmai-chenggu-tablets
#9
Peng Deng, Jianchun Zeng, Jie Li, Wenjun Feng, Jinlun Chen, Yirong Zeng
Avascular necrosis of the femoral head (ANFH) is an a frequently occurring orthopaedic disease with high morbidity. Traditional Chinese Medicine (TCM) Yuanshi Shengmai Chenggu Tablet is a valid prescription for treating steroid-induced femoral head necrosis. However, there are rare investigations about the serum protein marker expression after the acting of drugs on hormone and TCM. In the present study, we aimed to systematically discover and validate the serum biomarkers expression difference in patients with steroid-induced avascular necrosis of femoral head (SANFH) after taking Yuanshi Shengmai Chenggu Tablets (SANFH-TCM), so as to reveal the action mechanism of TCM from the molecular level by using isobaric tags for relative and absolute quantification (iTRAQ) with multiple reaction monitoring quantification...
2018: BioMed Research International
https://www.readbyqxmd.com/read/29749468/inhibition-of-cd9-expression-reduces-the-metastatic-capacity-of-human-hepatocellular-carcinoma-cell-line-mhcc97-h
#10
Qing Lin, Shifang Peng, Yixuan Yang
Metastasis is a characteristic of malignant tumors and may be a fatal clinical factor for many patients with cancer. Hepatocellular carcinoma (HCC) cells are highly metastatic; the mechanism of metastasis is complicated and may be influenced by a number of factors. Membrane proteins may block receptors or inhibit important enzymes, thus inhibiting tumor progression, and may be potential therapeutic targets for tumor prognosis and treatment. The present study aimed to use proteomics to analyze the dynamic changes of membrane proteins in HCC cells, to improve our understanding of membrane protein functions and to clarify the important components of the mechanisms of HCC metastasis...
April 26, 2018: International Journal of Oncology
https://www.readbyqxmd.com/read/29748923/proteomics-analysis-reveals-marker-proteins-for-minor-vein-initiation-in-rice-leaf
#11
Dan Feng, Yanwei Wang, Tiegang Lu, Zhiguo Zhang, Xiao Han
Leaf veins play a critical role in resource supplication and photosynthate translocation; thus, it is considered as an important agricultural trait for crop breeding. The rice minor veins are parallelly grown along all the parts of the leaf from base to tip. To understand the process of minor vein development, anatomy analysis was performed to reveal the initiation and development of minor veins in rice leaf. The frequency of minor vein initiation follows a decreased tendency from leaf base to tip. An iTRAQ-based proteomics analysis was performed in rice leaf sections...
May 11, 2018: Functional & Integrative Genomics
https://www.readbyqxmd.com/read/29746861/lenalidomide-regulates-osteocytes-fate-and-related-osteoclastogenesis-via-il-1%C3%AE-nf-%C3%AE%C2%BAb-rankl-signaling
#12
Xinhua Qu, Jingtian Mei, Zhifeng Yu, Zanjing Zhai, Han Qiao, Kerong Dai
Osteolytic diseases are closely associated with osteocyte fate, indicating a more efficient and crucial role of osteocyte-targeting strategy in inhibiting osteoclastogenesis. Here, we investigated the effects of lenalidomide (Lena) on osteocyte fate in order to regulate osteoclastogenesis via effective cascade-controlling response. Our data revealed that lenalidomide treatment notably rescued IL-1β induced loss of osteocyte viability by inhibiting osteocyte apoptosis with decreased osteoclast-related factors, RANKL and Sclerostin, as demonstrated by the restricted osteoclast formation and reduced bone resorption...
May 7, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29744368/identification-of-potential-therapeutic-targets-in-the-liver-of-pioglitazone-treated-type-2-diabetes-sprague-dawley-rats-via-expression-profile-chip-and-itraq-assay
#13
Zhong-Xia Lu, Wen-Jun Xu, Yang-Sheng Wu, Chang-Yu Li, Yi-Tao Chen
The aim of the present study was to identify key antidiabetic nodes in the livers of pioglitazone-treated type 2 diabetes mellitus Sprague-Dawley rats by transcriptomic and proteomic analysis. Rats were randomly divided into the control, the diabetes model, and the pioglitazone-treated groups. After treatment with pioglitazone for 11 weeks, the effects on fasting blood glucose, body weight, and blood biochemistry parameters were evaluated. Microarray and iTRAQ analysis were used to determine the differentially expressed genes/proteins in rat livers...
2018: Journal of Diabetes Research
https://www.readbyqxmd.com/read/29743870/absence-of-wdr13-gene-predisposes-mice-to-mild-social-isolation-chronic-stress-leading-to-depression-like-phenotype-associated-with-differential-expression-of-synaptic-proteins
#14
Shiladitya Mitra, Ghantasala S Sameer Kumar, B Jyothi Lakshmi, Suman Thakur, Satish Kumar
We earlier reported that the male mice lacking the Wdr13 gene ( Wdr13 -/0 ) showed mild anxiety, better memory retention, and up-regulation of synaptic proteins in the hippocampus. With increasing evidences from parallel studies in our laboratory about the possible role of Wdr13 in stress response, we investigated its role in brain. We observed that Wdr13 transcript gets up-regulated in the hippocampus of the wild-type mice exposed to stress. To further dissect its function, we analyzed the behavioral and molecular phenotypes of Wdr13 -/0 mice when subjected to mild chronic psychological stress, namely; mild (attenuated) social isolation...
2018: Frontiers in Molecular Neuroscience
https://www.readbyqxmd.com/read/29742327/membrane-proteome-of-invasive-retinoblastoma-differential-proteins-and-biomarkers
#15
Ravikanth Danda, Kalaivani Ganapathy, Gajanan Sathe, Anil K Madugundu, Uma Maheswari Krishnan, Vikas Khetan, Pukhraj Rishi, Harsha Gowda, Akhilesh Pandey, Krishnakumar Subramanian, Ts Keshava Prasad, Sailaja V Elchuri
PURPOSE: Retinoblastoma (RB) is a pediatric ocular cancer which is caused due to the aberrations in the RB1 gene. The changes in the membrane proteomics would help in understanding the development of the retinoblastoma and could identify candidates for biomarkers and therapy. EXPERIMENTAL DESIGN: We performed quantitative proteomics on the enriched membrane fractions from pooled normal retina (n = 5) and pooled retinoblastoma tissues (n = 5). The proteins were tryptic digested and tagged with iTRAQ labels...
May 9, 2018: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/29739330/proteome-wide-analysis-of-anopheles-culicifacies-mosquito-midgut-new-insights-into-the-mechanism-of-refractoriness
#16
Sonam Vijay, Ritu Rawal, Kavita Kadian, Jagbir Singh, Tridibesh Adak, Arun Sharma
BACKGROUND: Midgut invasion, a major bottleneck for malaria parasites transmission is considered as a potential target for vector-parasite interaction studies. New intervention strategies are required to explore the midgut proteins and their potential role in refractoriness for malaria control in Anopheles mosquitoes. To better understand the midgut functional proteins of An. culicifacies susceptible and refractory species, proteomic approaches coupled with bioinformatics analysis is an effective means in order to understand the mechanism of refractoriness...
May 8, 2018: BMC Genomics
https://www.readbyqxmd.com/read/29733959/itraq-based-proteomic-profile-analysis-of-isknv-infected-cpb-cells-with-emphasizing-on-glucose-metabolism-apoptosis-and-autophagy-pathways
#17
Shiwei Wu, Lujun Yu, Xiaozhe Fu, Xi Yan, Qiang Lin, Lihui Liu, Hongru Liang, Ningqiu Li
Infectious spleen and kidney necrosis virus (ISKNV) has caused significant losses in the cultured mandarin fish (Siniperca chuatsi) industry. The molecular mechanisms that underlie interaction between ISKNV and hosts are not fully understood. In this study, the proteomic profile of CPB cells at progressive time points after ISKNV infection was analyzed by isobaric tags for relative and absolute quantitation (iTRAQ). A total of 2731 proteins corresponding to 6363 novel peptides (false discovery rate <0.01) were identified...
May 4, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29730954/proteomic-analysis-identifies-tenascin-c-expression-is-upregulated-in-uterine-fibroids
#18
M Fairuz B Jamaluddin, Prathima B Nagendra, Pravin Nahar, Christopher Oldmeadow, Pradeep S Tanwar
Uterine leiomyomas (fibroids) are the most common gynecological tumors, which are enriched in the extracellular matrix (ECM). Fibroids are leading cause of abnormal uterine bleeding and hysterectomy. One of the major questions yet to be answered is the overproduction of specific ECM components in human uterine fibroids, particularly in relation to mutations in the driver gene mediator complex subunit 12 ( MED12). Surgical specimens from 14 patients with uterine leiomyoma having fibroids and corresponding adjacent normal myometrium (ANM) were utilized to analyze genetic and proteomic expression patterns in the tissue samples...
January 1, 2018: Reproductive Sciences
https://www.readbyqxmd.com/read/29729990/quantitative-proteomics-analysis-of-differentially-expressed-proteins-in-ruptured-and-unruptured-cerebral-aneurysms-by-itraq
#19
Pengjun Jiang, Jun Wu, Xin Chen, Bo Ning, Qingyuan Liu, Zhengsong Li, Maogui Li, Fan Yang, Yong Cao, Rong Wang, Shuo Wang
The underlying pathophysiological mechanisms involved in cerebral aneurysms rupture remain unclear. This study was performed to investigate the differentially expressed proteins between ruptured and unruptured aneurysms using quantitative proteomics. The aneurysmal walls of six ruptured aneurysms and six unruptured aneurysms were collected during the surgical operation. The isobaric tags for relative and absolute quantification (iTRAQ) were used to identify the differentially expressed proteins and western blotting was performed to validate the expression of the proteins of interest...
May 3, 2018: Journal of Proteomics
https://www.readbyqxmd.com/read/29729385/the-chinese-herbal-formula-zhibai-dihuang-granule-treat-yin-deficiency-heat-syndrome-rats-by-regulating-the-immune-responses
#20
Chang-Ming Liu, Jing Chen, Su Yang, Lian-Gen Mao, Ting-Ting Jiang, Hui-Hui Tu, Zhong-Liang Chen, Yu-Ting Hu, Lin Gan, Zhong-Jie Li, Ji-Cheng Li
ETHNOPHARMACOLOGICAL RELEVANCE: Zhibai Dihuang Granule (ZDG), a traditional Chinese medicine (TCM) made from eight Chinese herbs, has been classically used to treat Yin-deficiency-heat (YDH) syndrome. ZDG is well known with the therapeutic efficacy of nourishing Yin and decreasing internal heat in clinic, but the mechanism of ZDG's therapeutic effect is still not clear. MATERIALS AND METHODS: High doses of triiodothyronine (T3) were given intraperitoneally to induce Hyperthyroid YDH syndrome in SD rats...
May 2, 2018: Journal of Ethnopharmacology
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