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https://www.readbyqxmd.com/read/28918527/alterations-in-epididymal-proteomics-and-antioxidant-activity-of-mice-exposed-to-fluoride
#1
Zilong Sun, Sujuan Li, Yuxiang Yu, Hongyu Chen, Mohammad Mehdi Ommati, Ram Kumar Manthari, Ruiyan Niu, Jundong Wang
It is well known that high fluoride results in low fertility. Epididymis is the important place for spermatozoa maturation, which is essential for successful fertilization. In the previous studies, fluoride was reported to damage the epididymal structure of mouse and rabbit. However, the mechanism underlying sodium fluoride (NaF)-induced epididymal toxicity has not yet been well elucidated. The aim of this study is to explore the global protein alterations in epididymis of mice exposed to NaF using the iTRAQ technique...
September 16, 2017: Archives of Toxicology
https://www.readbyqxmd.com/read/28918157/differential-proteomic-profiling-of-endometrium-and-plasma-indicate-the-importance-of-hydrolysis-in-bovine-endometritis
#2
Shi-Dong Zhang, Shu-Wei Dong, Dong-Sheng Wang, Chike F Oguejiofor, Ali A Fouladi-Nashta, Zhi-Qiang Yang, Zuo-Ting Yan
Endometritis is an important disease of dairy cows that leads to significant economic losses in the dairy cattle industry. To investigate the alteration of proteins associated with endometritis in the dairy cow, the isobaric tags for relative and absolute quantification (iTRAQ) technique was applied to quantitatively identify differentially expressed proteins (DEP) in the endometrium and peripheral plasma of Chinese Holstein cows with endometritis. Compared with the normal (control) group, 159 DEP in the endometrium and 137 DEP in the plasma were identified in cows with endometritis...
September 13, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28917945/toxic-responses-of-perna-viridis-hepatopancreas-exposed-to-ddt-benzo-a-pyrene-and-their-mixture-uncovered-by-itraq-based-proteomics-and-nmr-based-metabolomics
#3
Qinqin Song, Hailong Zhou, Qian Han, Xiaoping Diao
Dichlorodiphenyltrichloroethane (DDT) and benzo(a)pyrene (BaP) are environmental estrogens (EEs) that are ubiquitous in the marine environment. In the present study, we integrated isobaric tags for relative and absolute quantitation (iTRAQ)-based proteomic and nuclear magnetic resonance (NMR)-based metabolomic approaches to explore the toxic responses of green mussel hepatopancreas exposed to DDT (10μg/L), BaP (10μg/L) and their mixture. The metabolic responses indicated that BaP primarily disturbed energy metabolism and osmotic regulation in the hepatopancreas of the male green mussel P...
September 11, 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/28912585/matrisome-profiling-during-intervertebral-disc-development-and-ageing
#4
Joana Caldeira, Cátia Santa, Hugo Osório, Maria Molinos, Bruno Manadas, Raquel Gonçalves, Mário Barbosa
Intervertebral disc (IVD) degeneration is often the cause of low back pain. Degeneration occurs with age and is accompanied by extracellular matrix (ECM) depletion, culminating in nucleus pulpous (NP) extrusion and IVD destruction. The changes that occur in the disc with age have been under investigation. However, a thorough study of ECM profiling is needed, to better understand IVD development and age-associated degeneration. As so, iTRAQ LC-MS/MS analysis of foetus, young and old bovine NPs, was performed to define the NP matrisome...
September 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28911185/identification-of-a-new-gene-yecc-involved-in-threonine-export-in-escherichia-coli
#5
Yiran Xu, Yongfei Liu, Feiran Li, Guoqiang Cao, Ping Zheng, Jibin Sun, Jianping Wen, Dawei Zhang
Threonine (Thr), an essential amino acid for mammals, has expanded its application to industries with fast-growing market demand. One factor that limits the production of L-threonine by microbial fermentation is the lack of an effective export system. This study proposes a method based on isobaric tags for relative and absolute quantification (iTRAQ) to determine potential Thr excretion proteins. Proteomics analysis revealed that an ABC family transport protein named YecC was upregulated in response to Thr addition...
September 15, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28910601/identification-of-plasma-biomarkers-for-distinguishing-bipolar-depression-from-major-depressive-disorder-by-itraq-coupled-lc-ms-ms-and-bioinformatics-analysis
#6
Juanjuan Ren, Guoqing Zhao, Xiujia Sun, Hongmei Liu, Ping Jiang, Jun Chen, Zhiguo Wu, Daihui Peng, Yiru Fang, Chen Zhang
It is important to differentiate between bipolar disorder (BD) and major depressive disorder (MDD) in the first depressive episode because of the potential treatment implications. Previous studies have mainly focused on the different clinical features or pathological biomarkers to distinguish these two diseases; however, a better understanding of the proteomics profiling of BD may help aid future therapeutic strategies. Here, we applied isobaric tags for relative and absolute quantification (iTRAQ) technology combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS) to identify differentially expressed proteins between MDD and bipolar depression (BP)...
September 5, 2017: Psychoneuroendocrinology
https://www.readbyqxmd.com/read/28910417/differential-cellular-responses-associated-with-oxidative-stress-and-cell-fate-decision-under-nitrate-and-phosphate-limitations-in-thalassiosira-pseudonana-comparative-proteomics
#7
Qun Lin, Jun-Rong Liang, Qian-Qian Huang, Chun-Shan Luo, Donald M Anderson, Chris Bowler, Chang-Ping Chen, Xue-Song Li, Ya-Hui Gao
Diatoms are important components of marine ecosystems and contribute greatly to the world's primary production. Despite their important roles in ecosystems, the molecular basis of how diatoms cope with oxidative stress caused by nutrient fluctuations remains largely unknown. Here, an isobaric tags for relative and absolute quantitation (iTRAQ) proteomic method was coupled with a series of physiological and biochemical techniques to explore oxidative stress- and cell fate decision-related cellular and metabolic responses of the diatom Thalassiosira pseudonana to nitrate (N) and inorganic phosphate (P) stresses...
2017: PloS One
https://www.readbyqxmd.com/read/28910323/comparative-proteomic-analyses-reveal-the-proteome-response-to-short-term-drought-in-italian-ryegrass-lolium-multiflorum
#8
Ling Pan, Zhongfu Yang, Jianping Wang, Pengxi Wang, Xiao Ma, Meiliang Zhou, Ji Li, Nie Gang, Guangyan Feng, Junming Zhao, Xinquan Zhang
Drought is a major abiotic stress that impairs growth and productivity of Italian ryegrass. Comparative analysis of drought responsive proteins will provide insight into molecular mechanism in Lolium multiflorum drought tolerance. Using the iTRAQ-based approach, proteomic changes in tolerant and susceptible lines were examined in response to drought condition. A total of 950 differentially accumulated proteins was found to be involved in carbohydrate metabolism, amino acid metabolism, biosynthesis of secondary metabolites, and signal transduction pathway, such as β-D-xylosidase, β-D-glucan glucohydrolase, glycerate dehydrogenase, Cobalamin-independent methionine synthase, glutamine synthetase 1a, Farnesyl pyrophosphate synthase, diacylglycerol, and inositol 1, 4, 5-trisphosphate, which might contributed to enhance drought tolerance or adaption in Lolium multiflorum...
2017: PloS One
https://www.readbyqxmd.com/read/28906478/complementary-rna-sequencing-based-transcriptomics-and-itraq-proteomics-reveal-the-mechanism-of-the-alleviation-of-quinclorac-stress-by-salicylic-acid-in-oryza-sativa-ssp-japonica
#9
Jian Wang, Faisal Islam, Lan Li, Meijuan Long, Chong Yang, Xiaoli Jin, Basharat Ali, Bizeng Mao, Weijun Zhou
To uncover the alleviation mechanism of quinclorac stress by salicylic acid (SA), leaf samples of Oryza sativa ssp. Japonica under quinclorac stress with and without SA pre-treatment were analyzed for transcriptional and proteomic profiling to determine the differentially expressed genes (DEGs) and proteins (DEPs), respectively. Results showed that quinclorac stress altered the expression of 2207 DEGs (1427 up-regulated, 780 down-regulated) and 147 DEPs (98 down-regulated, 49 up-regulated). These genes and proteins were enriched in glutathione (GSH) metabolism, porphyrin and chlorophyll metabolism, the biosynthesis of secondary metabolites, glyoxylate and dicarboxylate metabolism, and so on...
September 14, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28895652/quantitative-proteomics-and-targeted-fatty-acids-analysis-reveal-the-damage-of-triptolide-in-liver-and-kidney
#10
Menglin Li, Ting Hu, Cai Tie, Liang Qu, Hao Zheng, Jinlan Zhang
Triptolide (TP), the major active component in Tripterygium wilfordii Hook. f., is widely used for the treatment of rheumatoid arthritis and autoimmune disease. However, organ toxicity, especially hepatotoxicity and nephrotoxicity, limits its clinical application. To fully understand the mechanism underlying TP toxicity, iTRAQ-based 2D-LC-MS/MS proteomics was used to detect differentially expressed proteins in the livers and kidneys of mice administered the LD50 dose of TP. Functional annotation revealed that multiple pathways are involved in TP toxicity, including acute-phase response signaling, the antigen presentation pathway, FXR/RXR activation, LPS/IL-1-mediated inhibition of RXR function and EIF2 signaling...
September 12, 2017: Proteomics
https://www.readbyqxmd.com/read/28887600/strong-sudden-cooling-alleviates-the-inflammatory-responses-in-heat-stressed-dairy-cows-based-on-itraq-proteomic-analysis
#11
Jianbo Cheng, Li Min, Nan Zheng, Caiyun Fan, Shengguo Zhao, Yangdong Zhang, Jiaqi Wang
This study was designed to investigate the effects of sudden cooling on the physiological responses of 12 heat-stressed Holstein dairy cows using an isobaric tags for relative and absolute quantification (iTRAQ) labeling approach. Plasma samples were collected from these cows during heat stress (HS), and after strong, sudden cooling in the summer (16 days later). We compared plasma proteomic data before and after sudden cooling to identify the differentially abundant proteins. The results showed that sudden cooling in summer effectively alleviated the negative consequences of HS on body temperature and production variables...
September 9, 2017: International Journal of Biometeorology
https://www.readbyqxmd.com/read/28887495/integration-of-deep-transcriptome-and-proteome-analyses-of-salicylic-acid-regulation-high-temperature-stress-in-ulva-prolifera
#12
Meihua Fan, Xue Sun, Nianjun Xu, Zhi Liao, Yahe Li, Jianxin Wang, Yingping Fan, Dalian Cui, Peng Li, Zengliang Miao
To investigate changes in transcript and relative protein levels in response to salicylic acid regulation of the thermotolerance in U. prolifera, complementary transcriptome and proteome analyses were performed with U. prolifera grown at 35 °C (UpHT) and with the addition of SA at high temperature (UpSHT). At mRNA level,12,296 differentially expressed genes (DEGs) were obtained from the comparison of UpSHT with UpHT. iTRAQ-labeling proteome analysis showed that a total of 4,449 proteins were identified and reliably quantified...
September 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28886312/differences-in-expression-of-serum-protein-in-patients-with-psoriasis-vulgaris-and-blood-heat-syndrome-and-healthy-volunteers
#13
P Hao, C Yang, X Yan, T Li, J Zhang, Q Yu, X Zhang, Y Zhang
To explore the mechanism of psoriasis vulgaris (PV), serum protein expression profiles between PV patients with blood-heat syndrome and healthy volunteers were detected by isobaric tags for relative and absolute quantitation (iTRAQ). First, sera from 15 PV patients with blood-heat syndrome and 10 healthy volunteers were collected; then, serum proteins were separated and hydrolyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and a specific iTRAQ marker enzyme respectively after further purification and protein abundance treatment...
August 30, 2017: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/28885018/molecular-mechanisms-of-perfluorooctanoate-induced-hepatocyte-apoptosis-in-mice-using-proteomic-techniques
#14
Kan Li, Jie Sun, Jingping Yang, Stephen M Roberts, Xu-Xiang Zhang, Xinyi Cui, Si Wei, Lena Q Ma
The stability coupled with its wide use of perfluorooctanoate (PFOA) cause serious concern regarding its potential risks to human health. The molecular mechanisms of PFOA-induced hepatotoxicity relevant to human health was investigated by using both in vivo (mice model) and in vitro (human hepatocyte cells, HL-7702) techniques. Both male and female Balb/c mice were administered with PFOA at 0.05, 0.5, or 2.5 mg/kg-d for 28-d, which induced serum concentrations at environmental relevance levels. Liver samples were examined for histology and proteomic change using iTRAQ and Western Blotting, showing dose-dependent hepatocyte apoptosis and peroxisome proliferation...
September 8, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28884822/rna-binding-protein-musashi-2-regulates-piwil1-and-tbx1-in-mouse-spermatogenesis
#15
Jessie M Sutherland, Alexander P Sobinoff, Barbara A Fraser, Kate A Redgrove, Nicole A Siddall, Peter Koopman, Gary R Hime, Eileen A McLaughlin
RNA-binding proteins (RBP) are important facilitators of post-transcriptional gene regulation. We have previously established that nuclear overexpression of the RBP Musashi-2 (MSI2) during male germ cell maturation is detrimental to sperm cell development and fertility. Herein we determine the genes and pathways impacted by the upregulation of Msi2. Microarray analysis and qPCR confirmed differential gene expression in factors fundamental to the cell cycle, cellular proliferation, and cell death. Similarly, comparative protein expression analysis via iTRAQ, immunoblot, and immunolocalisation, identified differential expression and localisation of important regulators of transcription, translation, RNA processing, and spermatogenesis...
September 8, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28883884/itraq-based-quantitative-proteomics-analysis-of-the-protective-effect-of-yinchenwuling-powder-on-hyperlipidemic-rats
#16
Zheyu Zhang, Wenbo Wang, Ling Jin, Xin Cao, Gonghui Jian, Ning Wu, Xia Xu, Ye Yao, Dongsheng Wang
Yinchenwuling powder (YCL) is an effective traditional Chinese medicine formula to modulate lipid levels. In this study, we established hyperlipidemic rat models and treated them with YCL. The serum concentrations of lipid, malondialdehyde (MDA), endothelin-1 (ET-1), and calcitonin gene-related peptide (CGRP) were measured. Adventitia-free vascular proteins between hyperlipidemic rats and YCL-treated rats were identified using iTRAQ-based quantitative proteomics research approach. Proteins with 1.3-fold difference were analyzed through bioinformatics, and proteomic results were verified by Western blot...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/28877517/the-histopathological-characteristics-caused-by-trionyx-sinensis-hemorrhagic-syndrome-virus-tshsv-and-comparative-proteomic-analysis-of-liver-tissue-in-tshsv-infected-chinese-soft-shelled-turtles-pelodiscus-sinensis
#17
Li Liu, Zheng Cao, Feng Lin, Xueping Ye, Shujuan Lu, Sunjian Lyv
Trionyx sinensis hemorrhagic syndrome virus (TSHSV) is a pathogen that causes severe hemorrhagic syndrome and irreversible damage to different infected tissues of Pelodis cus sinensis, ending in the death of affected organisms. In the present study, the histopathological characteristics of TSHSV-infected P. sinensis were analyzed and compared by HE staining. Relative and absolute quantification (iTRAQ)-based proteomic analysis was employed to explore the molecular pathology of liver injury. Anatomical features indicated that TSHSV caused obvious congestion in the liver, kidney, intestine, and other tissues of P...
September 7, 2017: Intervirology
https://www.readbyqxmd.com/read/28873731/comparative-proteomic-analysis-of-cronobacter-sakazakii-by-itraq-provides-insights-into-response-to-desiccation
#18
Shuangfang Hu, Yigang Yu, Xinwei Wu, Xingzhou Xia, Xinglong Xiao, Hui Wu
Cronobacter sakazakii is a foodborne pathogen throughout the world and survives extremely desiccation stress. However, the molecular basis involved in desiccation resistance of C. sakazakii is still unknown. In this study, the potential desiccation resistance factors of C. sakazakii ATCC 29544 were determined using iTRAQ-based quantitative proteomic analysis. A total of 2775 proteins were identified by iTRAQ, of which 233 showed a different protein expression between control group and desiccation stress group...
October 2017: Food Research International
https://www.readbyqxmd.com/read/28873618/unravelling-proteome-changes-of-chicken-egg-whites-under-carbon-dioxide-modified-atmosphere-packaging
#19
Lei Xu, Fei Jia, Changyao Luo, Qianqian Yu, Ruitong Dai, Xingmin Li
Unfertilized chicken eggs within 24h of laying were chosen and stored at 25°C and 45% humidity for 0, 20, and 40days. The experimental group (EG) was the carbon dioxide-modified atmosphere packaging (CDMAP) group, whereas the control group (CG) contained eggs without special handling. Egg freshness indexes were measured. The proteome of the egg whites was determined by LC-MS/MS using isobaric tags for relative and absolute quantitation (iTRAQ). A total of 87 proteins were detected. The results indicated that CDMAP can control the change in protein abundance...
January 15, 2018: Food Chemistry
https://www.readbyqxmd.com/read/28871227/comparative-proteomic-analysis-provides-insight-into-the-key-proteins-as-possible-targets-involved-in-aspirin-inhibiting-biofilm-formation-of-staphylococcus-xylosus
#20
Chang-Geng Xu, Yan-Bei Yang, Yong-Hui Zhou, Mei-Qi Hao, Yong-Zhi Ren, Xiao-Ting Wang, Jian-Qing Chen, Ishfaq Muhammad, Shuai Wang, Di Liu, Xiu-Bo Li, Yan-Hua Li
Staphylococcus xylosus is an opportunistic pathogen that causes infection in humans and cow mastitis. And S. xylosus possesses a strong ability to form biofilms in vitro. As biofilm formation facilitates resistance to antimicrobial agents, the discovery of new medicinal properties for classic drugs is highly desired. Aspirin, which is the most common active component of non-steroidal anti-inflammatory compounds, affects the biofilm-forming capacity of various bacterial species. We have found that aspirin effectively inhibits biofilm formation of S...
2017: Frontiers in Pharmacology
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