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https://www.readbyqxmd.com/read/28087900/itraq-based-quantitative-proteomic-analysis-and-bioinformatics-study-of-proteins-in-pterygia
#1
Dandan Linghu, Lili Guo, Yinghua Zhao, Zhiming Liu, Mingwei Zhao, Lvzhen Huang, Xiaoxin Li
PURPOSE: To analysis proteins in the tissue of pterygia, and to investigate their potential roles in pterygia, using the comparative proteomic technique of Isobaric Tags for Relative and Absolute Quantitation (iTRAQ) coupled with offline 2DLC-MS/MS, Western-bolt. METHOD: The tissues of pterygia and healthy conjunctiva were collected from 10 pterygia patients (6 females, 4 males; average age was 52 years old; average course of disease was 6 years) in our hospital from September, 2015 to March, 2016...
January 13, 2017: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/28087279/alpha-2-macroglobulin-and-heparin-cofactor-ii-and-the-vulnerability-of-carotid-atherosclerotic-plaques-an-itraq-based-analysis
#2
Jinmao Li, Xi Liu, Ying Xiang, Xueying Ding, Teng Wang, Ying Liu, Maojia Yin, Changhong Tan, Fen Deng, Lifen Chen
Rupture of carotid atherosclerotic plaque may cause stroke, while few biomarker in clinic can evaluate carotid plaque vulnerability. In this study, we divided the recruited participants into no plaque, stable plaque, and vulnerable plaque group according to carotid ultrasonography, and screened the differentially expressed proteins in plasma of these participants using isobaric tags for relative and absolute quantitation labeling coupled with liquid chromatography-tandem mass spectrometry. 28 proteins were identified differentially expressed, among which alpha-2-macroglobulin (α2M) and heparin cofactor II (HCII) were found to be at hub position in the interactions of these proteins by STRING analysis and were selected for enzyme-linked immunosorbent assay measurement to assess their relevance with carotid plaques vulnerability and diagnostic efficiency...
January 10, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28079114/discovering-potential-serological-biomarker-for-chronic-hepatitis-b-virus-related-hepatocellular-carcinoma-in-chinese-population-by-mal-associated-serum-glycoproteomics-analysis
#3
Tianhua Liu, Denghe Liu, Riqiang Liu, Hucong Jiang, Guoquan Yan, Wei Li, Lu Sun, Shu Zhang, Yinkun Liu, Kun Guo
The accuracy of current biomarkers for the diagnosis of hepatocellular carcinoma (HCC), especially chronic Hepatitis B Virus (HBV)-related HCC, is limited. Recent progress in glycoproteomics has provided a novel platform for screening novel serological biomarkers of HCC. In this study, lectin affinity chromatography by Maackia amurensis lectin (MAL) and iTRAQ combined with mass spectrometric analysis were performed to enrich and identify the glycoprotein fractions in serum samples from HBV-related HCC patients and from healthy controls...
January 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28073423/-research-advances-in-application-of-isobaric-tags-for-relative-and-absolute-quantitation-in-proteomics-of-hepatocellular-carcinoma
#4
Y Z Qi, L S Chen, P Xu
Due to the features of strong heterogeneity, difficult early diagnosis, poor prognosis, and high fatality rate, hepatocellular carcinoma (HCC) has become an important disease which threatens the health of the Chinese population. Accurate early diagnosis is crucial to improving the success rate of liver cancer resection and reducing postoperative recurrence and metastasis, and its core is the screening and validation of biomarkers for early diagnosis. Isobaric tags for relative and absolute quantitation (iTRAQ) and stable isotope labeling with amino acids in cell culture are important parts of proteomics technology, and iTRAQ has become the most important technique in quantitative proteomics technology due to its advantages of high throughput, high quantitative accuracy, and no limitation by sample source...
December 20, 2016: Zhonghua Gan Zang Bing za Zhi, Zhonghua Ganzangbing Zazhi, Chinese Journal of Hepatology
https://www.readbyqxmd.com/read/28068390/effect-of-inulin-on-proteome-changes-induced-by-pathogenic-lipopolysaccharide-in-human-colon
#5
Michele Pier Luca Guarino, Annamaria Altomare, Simone Barera, Vittoria Locato, Silvia Cocca, Cinzia Franchin, Giorgio Arrigoni, Candida Vannini, Sarah Grossi, Paola Campomenosi, Valentina Pasqualetti, Marcella Bracale, Rossana Alloni, Laura De Gara, Michele Cicala
In the present study, the protective role of inulin against lipopolysaccharide (LPS)-induced oxidative stress was evaluated on human colonic mucosa using a proteomic approach. Human colonic mucosa and submucosa were sealed between two chambers, with the luminal side facing upwards and overlaid with Krebs (control), LPS or LPS+ inulin IQ solution. The solutions on the submucosal side (undernatants) were collected following 30 min of mucosal exposure. iTRAQ based analysis was used to analyze the total soluble proteomes from human colonic mucosa and submucosa treated with different undernatants...
2017: PloS One
https://www.readbyqxmd.com/read/28056817/proteomic-analyses-of-limbic-regions-in-neonatal-male-female-and-androgen-receptor-knockout-mice
#6
Anna Zettergren, Sara Karlsson, Erik Studer, Anna Sarvimäki, Petronella Kettunen, Annika Thorsell, Carina Sihlbom, Lars Westberg
BACKGROUND: It is well-established that organizational effects of sex steroids during early development are fundamental for sex-typical displays of, for example, mating and aggressive behaviors in rodents and other species. Male and female brains are known to differ with respect to neuronal morphology in particular regions of the brain, including the number and size of neurons, and the density and length of dendrites in nuclei of hypothalamus and amygdala. The aim of the present study was to use global proteomics to identify proteins differentially expressed in hypothalamus/amygdala during early development (postnatal day 8) of male, female and conditional androgen receptor knockout (AR(NesDel)) male mice, lacking androgen receptors specifically in the brain...
January 5, 2017: BMC Neuroscience
https://www.readbyqxmd.com/read/28056797/comparative-proteomic-analysis-reveals-a-dynamic-pollen-plasma-membrane-protein-map-and-the-membrane-landscape-of-receptor-like-kinases-and-transporters-important-for-pollen-tube-growth-and-interaction-with-pistils-in-rice
#7
Ning Yang, Tai Wang
BACKGROUND: The coordination of pollen tube (PT) growth, guidance and timely growth arrest and rupture mediated by PT-pistil interaction is crucial for the PT to transport sperm cells into ovules for double fertilization. The plasma membrane (PM) represents an important interface for cell-cell interaction, and PM proteins of PTs are pioneers for mediating PT integrity and interaction with pistils. Thus, understanding the mechanisms underlying these events is important for proteomics. RESULTS: Using the efficient aqueous polymer two-phase system and alkali buffer treatment, we prepared high-purity PM from mature and germinated pollen of rice...
January 5, 2017: BMC Plant Biology
https://www.readbyqxmd.com/read/28045158/metabolomics-and-proteomics-technologies-to-explore-the-herbal-preparation-affecting-metabolic-disorders-using-high-resolution-mass-spectrometry
#8
Aihua Zhang, Xiaohang Zhou, Hongwei Zhao, Shiyu Zou, Chung Wah Ma, Qi Liu, Hui Sun, Liang Liu, Xijun Wang
An integrative metabolomics and proteomics approach can provide novel insights in the understanding of biological systems. We have integrated proteome and metabolome data sets for a holistic view of the molecular mechanisms in disease. Using quantitative iTRAQ-LC-MS/MS proteomics coupled with UPLC-Q-TOF-HDMS based metabolomics, we determined the protein and metabolite expression changes in the kidney-yang deficiency syndrome (KYDS) rat model and further investigated the intervention effects of the Jinkui Shenqi Pill (JSP)...
January 3, 2017: Molecular BioSystems
https://www.readbyqxmd.com/read/28042969/the-response-of-growth-and-patulin-production-of-postharvest-pathogen-penicillium-expansum-to-exogenous-potassium-phosphite-treatment
#9
Tongfei Lai, Ying Wang, Yaya Fan, Yingying Zhou, Ying Bao, Ting Zhou
In this study, the effects of exogenous potassium phosphite (Phi) on growth and patulin production of postharvest pathogen Penicillium expansum were assessed. The results indicated that P. expansum under 5mmol/L Phi stress presented obvious development retardation, yield reduction of patulin and lower infectivity to apple fruit. Meanwhile, expression analysis of 15 genes related to patulin biosynthesis suggested that Phi mainly affected the early steps of patulin synthetic route at transcriptional level. Furthermore, a global view of proteome and transcriptome alteration of P...
December 28, 2016: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28039103/itraq-based-proteome-analysis-of-fluoroquinolone-resistant-staphylococcus-aureus
#10
Van Chi Thai, Teck Kwang Lim, Kim Phuong Uyen Le, Qingsong Lin, Thi Thu Hoai Nguyen
OBJECTIVES: The aim of this study was to compare global protein expression changes during fluoroquinolone (FQ) exposure of Staphylococcus aureus. METHODS: Total protein extracts of wild-type S. aureus ATCC 29213 and six multidrug-resistant (MDR) strains derived from the wild-type under different FQ exposures were analysed using the 8-plex isobaric tag for relative and absolute quantitation (iTRAQ) method combined with LC-MS/MS analysis. Differentially expressed proteins were searched for their Gene Ontology (GO) annotation (UniProt database) and protein-protein interaction network (STRING v...
December 27, 2016: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28036116/hmgb1-down-regulation-mediates-terameprocol-vascular-anti-proliferative-effect-in-experimental-pulmonary-hypertension
#11
Rita Nogueira-Ferreira, Manuel J Ferreira-Pinto, Ana Filipa Silva, Rui Vitorino, Joana Justino, Raquel Costa, Daniel Moreira-Gonçalves, Jean-François Quignard, Thomas Ducret, Jean-Pierre Savineau, Adelino F Leite-Moreira, Rita Ferreira, Tiago Henriques-Coelho
Pulmonary arterial hypertension (PAH) is a progressive disease with a poor prognosis. Pulmonary artery smooth muscle cells (PASMCs) play a crucial role in PAH pathophysiology, displaying a hyperproliferative and apoptotic-resistant phenotype. In the present study, we evaluated the potential therapeutic role of terameprocol (TMP), an inhibitor of cellular proliferation and promoter of apoptosis, in a well-established pre-clinical model of PAH induced by monocrotaline (MCT) and studied the biological pathways modulated by TMP in PASMCs...
December 30, 2016: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28025641/itraq-based-proteomics-identification-of-serum-biomarkers-of-two-chronic-hepatitis-b-subtypes-diagnosed-by-traditional-chinese-medicine
#12
Jiankun Yang, Lichao Yang, Baixue Li, Weilong Zhou, Sen Zhong, Zhenhua Zhuang, Bin Yang, Maoshan Chen, Quansheng Feng
Background. Chronic infection with hepatitis B virus (HBV) is a leading cause of cirrhosis and hepatocellular carcinoma. By traditional Chinese medicine (TCM) pattern classification, damp heat stasis in the middle-jiao (DHSM) and liver Qi stagnation and spleen deficiency (LSSD) are two most common subtypes of CHB. Results. In this study, we employed iTRAQ proteomics technology to identify potential serum protein biomarkers in 30 LSSD-CHB and 30 DHSM-CHB patients. Of the total 842 detected proteins, 273 and 345 were differentially expressed in LSSD-CHB and DHSM-CHB patients compared to healthy controls, respectively...
2016: BioMed Research International
https://www.readbyqxmd.com/read/28018404/functional-and-integrative-analysis-of-the-proteomic-profile-of-radish-root-under-pb-exposure
#13
Yan Wang, Liang Xu, Mingjia Tang, Haiyan Jiang, Wei Chen, Wei Zhang, Ronghua Wang, Liwang Liu
Lead (Pb) is one of the most abundant heavy metal (HM) pollutants, which can penetrate the plant through the root and then enter the food chain causing potential health risks for human beings. Radish is an important root vegetable crop worldwide. To investigate the mechanism underlying plant response to Pb stress in radish, the protein profile changes of radish roots respectively upon Pb(NO3)2 at 500 mg L(-1)(Pb500) and 1000 mg L(-1)(Pb1000), were comprehensively analyzed using iTRAQ (Isobaric Tag for Relative and Absolute Quantification)...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28009970/combined-serum-and-eps-urine-proteomic-analysis-using-itraq-technology-for-discovery-of-potential-prostate-cancer-biomarkers
#14
Mo Zhang, Lizhu Chen, Zhengwei Yuan, Zeyu Yang, Yue Li, Liping Shan, Bo Yin, Xiang Fei, Jianing Miao, Yongsheng Song
Prostate cancer (PCa) is one of the most common malignant tumors and a major cause of cancer-related death for men worldwide. The aim of our study was to identify potential non-invasive serum and expressed prostatic secretion (EPS)-urine biomarkers for accurate diagnosis of PCa. Here, we performed a combined isobaric tags for relative and absolute quantification (iTRAQ) proteomic analysis to compare protein profiles using pooled serum and EPS-urine samples from 4 groups of patients: benign prostate hyperplasia (BPH), high grade prostatic intraepithelial neoplasia (HGPIN), localized PCa and metastatic PCa...
November 2016: Discovery Medicine
https://www.readbyqxmd.com/read/28008332/quantitative-proteomic-analyses-identify-aba-related-proteins-and-signal-pathways-in-maize-leaves-under-drought-conditions
#15
Yulong Zhao, Yankai Wang, Hao Yang, Wei Wang, Jianyu Wu, Xiuli Hu
Drought stress is one of major factors resulting in maize yield loss. The roles of abscisic acid (ABA) have been widely studied in crops in response to drought stress. However, more attention is needed to identify key ABA-related proteins and also gain deeper molecular insights about drought stress in maize. Based on this need, the physiology and proteomics of the ABA-deficient maize mutant vp5 and its wild-type Vp5 under drought stress were examined and analyzed. Malondialdehyde content increased and quantum efficiency of photosystem II decreased under drought stress in both genotypes...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28006025/itraq-based-quantitative-proteomics-identifies-potential-regulatory-proteins-involved-in-chicken-eggshell-brownness
#16
Guangqi Li, Congjiao Sun, Guiqin Wu, Fengying Shi, Aiqiao Liu, Ning Yang
Brown eggs are popular in many countries and consumers regard eggshell brownness as an important indicator of egg quality. However, the potential regulatory proteins and detailed molecular mechanisms regulating eggshell brownness have yet to be clearly defined. In the present study, we performed quantitative proteomics analysis with iTRAQ technology in the shell gland epithelium of hens laying dark and light brown eggs to investigate the candidate proteins and molecular mechanisms underlying variation in chicken eggshell brownness...
2016: PloS One
https://www.readbyqxmd.com/read/28005350/responses-of-the-proteome-and-metabolome-in-livers-of-zebrafish-exposed-chronically-to-environmentally-relevant-concentrations-of-microcystin-lr
#17
Liang Chen, Yufei Hu, Jun He, Jun Chen, John P Giesy, Ping Xie
In this study, for the first time, changes in expressions of proteins and profiles of metabolites in liver of the small, freshwater fish [Formula: see text] (zebrafish) were investigated after long-term exposure to environmentally relevant concentrations of microcystin-LR (MC-LR). Male zebrafish were exposed via water to 1 or 10 μg MC-LR/L for 90 days, and iTRAQ-based proteomics and (1)H NMR-based metabolomics were employed. Histopathological observations showed that MC-LR caused damage to liver, and the effects were more pronounced in fish exposed to 10 μg MC-LR/L...
January 3, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28003598/an-itraq-based-quantitative-proteomics-study-of-refractory-mycoplasma-pneumoniae-pneumonia-patients
#18
Jia-Lu Yu, Qi-Fang Song, Zhi-Wei Xie, Wen-Hui Jiang, Jia-Hui Chen, Hui-Feng Fan, Ya-Ping Xie, Gen Lu
Mycoplasma pneumoniae(MP) is a leading cause of community-acquired pneumonia in children and young adults. Although MP pneumonia is usually benign and self-limited, in some cases it can develop into a life-threating refractory MP pneumonia(RMPP). However, the pathogenesis of RMPP is poorly understood. The identification and characterization of proteins related to RMPP could provide "proof of principle" to facilitate appropriate diagnostic and therapeutic strategies for MP patients. In this study, we applied the quantitative proteomic technique(iTRAQ) to globally analyze MP-related proteins in serum samples from five RMPP and five non-refractory MP pneumonia(NRMPP) patients, and five healthy children...
December 22, 2016: Japanese Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28000706/identification-of-a-tnf-%C3%AE-inducer-mic3-originating-from-the-microneme-of-non-cystogenic-virulent-toxoplasma-gondii
#19
Jingfan Qiu, Lijuan Wang, Rong Zhang, Ke Ge, Hongfei Guo, Xinjian Liu, Jinfeng Liu, Delong Kong, Yong Wang
Toxoplasma gondii is an opportunistic parasite with avirulent cystogenic and highly virulent non-cystogenic isolates. Although non-cystogenic strains are considered the most virulent, there are also marked genetic and virulence differences among these strains. Excretory-secretory antigens (ESAs) of T. gondii are critical for the invasion process and the immune response of the host. To better understand the differences in virulence between non-cystogenic T. gondii isolates, we studied ESAs of the RH strain (Type I), and the very prevalent in China, but less virulent TgCtwh3 strain (Chinese 1)...
December 21, 2016: Scientific Reports
https://www.readbyqxmd.com/read/28000052/itraq-based-quantitative-proteomics-reveals-important-host-factors-involved-in-the-high-pathogenicity-of-the-h5n1-avian-influenza-virus-in-mice
#20
Jiao Hu, Zhao Gao, Xiaoquan Wang, Min Gu, Yanyan Liang, Xiaowen Liu, Shunlin Hu, Huimou Liu, Wenbo Liu, Sujuan Chen, Daxin Peng, Xiufan Liu
We previously reported a pair of H5N1 avian influenza viruses which are genetically similar but differ greatly in their virulence in mice. A/Chicken/Jiangsu/k0402/2010 (CK10) is highly lethal to mice, whereas A/Goose/Jiangsu/k0403/2010 (GS10) is avirulent. In this study, to investigate the host factors that account for their virulence discrepancy, we compared the pathology and host proteome of the CK10- or GS10-infected mouse lung. Moderate lung injury was observed from CK10-infected animals as early as the first day of infection, and the pathology steadily progressed at later time point...
December 20, 2016: Medical Microbiology and Immunology
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