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https://www.readbyqxmd.com/read/29345284/quantitative-proteomics-analysis-of-the-role-of-tetraspanin-8-in-the-drug-resistance-of-gastric-cancer
#1
Lan Li, Daping Yang, Dejun Cui, Yu Li, Zhao Nie, Jinglin Wang, Li Liang
Gastric cancer, due to its high incidence rate, is the second leading cause of cancer-related mortality worldwide. Chemotherapy is an important component of the multimodal treatment for gastric cancer; however, a significant impediment to successful treatment is multidrug resistance (MDR) in patients with gastric cancer. In the present study, the protein profiles of the MDR cell line, SGC7901/DDP, and its parental cell line, SGC7901, were comparatively analyzed through an iTRAQ-based quantitative proteomics technique...
February 2018: International Journal of Oncology
https://www.readbyqxmd.com/read/29344252/itraq-based-quantitative-proteomic-analysis-and-bioinformatics-study-of-proteins-in-retinoblastoma
#2
Yong Cheng, Qingyu Meng, Lvzhen Huang, Xuan Shi, Jing Hou, Xiaoxin Li, Jianhong Liang
The aim of the present study was to analyze proteins in the aqueous humor (AH) of patients' retinoblastoma (RB), and investigate their potential role in RB using the comparative proteomic technique of isobaric tags for relative and absolute quantitation (iTRAQ) coupled with offline two-dimensional liquid chromatography-tandem mass spectrometry. A total of 0.1 ml AH was collected from 10 children with RB (mean age, 3.8 years; range, 2-5 years) and patients with senile cataracts (mean age, 70.4 years; range, 65-79 years), which was used as the control...
December 2017: Oncology Letters
https://www.readbyqxmd.com/read/29339339/proteomics-and-genetic-analyses-reveal-the-effects-of-arsenite-oxidation-on-metabolic-pathways-and-the-roles-of-aior-in-agrobacterium-tumefaciens-gw4
#3
Kaixiang Shi, Qian Wang, Xia Fan, Gejiao Wang
A heterotrophic arsenite [As(III)]-oxidizing bacterium Agrobacterium tumefaciens GW4 isolated from As(III)-rich groundwater sediment showed high As(III) resistance and could oxidize As(III) to As(V). The As(III) oxidation could generate energy and enhance growth, and AioR was the regulator for As(III) oxidase. To determine the related metabolic pathways mediated by As(III) oxidation and whether AioR regulated other cellular responses to As(III), isobaric tags for relative and absolute quantitation (iTRAQ) was performed in four treatments, GW4 (+AsIII)/GW4 (-AsIII), GW4-ΔaioR (+AsIII)/GW4-ΔaioR (-AsIII), GW4-ΔaioR (-AsIII)/GW4 (-AsIII) and GW4-ΔaioR (+AsIII)/GW4 (+AsIII)...
January 12, 2018: Environmental Pollution
https://www.readbyqxmd.com/read/29334195/exoproteome-profiling-reveals-the-involvement-of-the-foldase-prsa-in-the-cell-surface-properties-and-pathogenesis-of-staphylococcus-aureus
#4
Mei-Hui Lin, Chi-Chun Li, Jwu-Ching Shu, Hao-Wei Chu, Chao-Chin Liu, Chih-Ching Wu
Staphylococcus aureus is a bacterial pathogen that produces and exports many virulence factors that cause diseases in human. PrsA, a membrane-bound foldase, is expressed ubiquitously in Gram-positive bacteria and required for the folding of exported proteins into a stable and active structure. To understand the involvement of PrsA in post-translocational protein folding in S. aureus, a PrsA-deficient mutant of S. aureus HG001 was constructed. Using isobaric tags for relative and absolute quantification (iTRAQ)-based mass spectrometry analyses, the exoproteomes of PrsA mutant and wild type S...
January 15, 2018: Proteomics
https://www.readbyqxmd.com/read/29329437/association-of-extracellular-dntp-utilization-with-a-gmpap1-like-protein-identified-in-cell-wall-proteomic-analysis-of-soybean-roots
#5
Weiwei Wu, Yan Lin, Pandao Liu, Qianqian Chen, Jiang Tian, Cuiyue Liang
Plant root cell walls are dynamic systems that serve as the first plant compartment responsive to soil conditions, such as phosphorus (P) deficiency. To date, evidence for the regulation of root cell wall proteins (CWPs) by P deficiency remains sparse. In order to gain a better understanding of the roles played by CWPs in the roots of soybean (Glycine max) in adaptation to P deficiency, we conducted an iTRAQ (isobaric tag for relative and absolute quantitation) proteomic analysis. A total of 53 CWPs with differential accumulation in response to P deficiency were identified...
January 10, 2018: Journal of Experimental Botany
https://www.readbyqxmd.com/read/29327447/itraq-based-proteomic-analysis-explore-the-influence-of-hypoxia-on-the-proteome-of-dental-pulp-stem-cells-under-3d-culture
#6
Lei Dou, Qifang Yan, Panpan Liang, Pengfei Zhou, Yan Zhang, Ping Ji
Various approaches are used to improve the efficacy of mesenchymal stem cells(MSCs) therapy. Hypoxic preconditioning is one of the most commonly-applied strategy prior to MSCs transplantation for enhancing the therapeutic effect. Identifying the effect of hypoxic preconditioning is helpful for better application. However, the influence of hypoxia on dental tissue derived MSCs cultured in 3D was lacking. Thus, this present study is to investigate the gene expression and proteome of dental pulp stem cells(DPSCs) after hypoxic preconditioning...
January 12, 2018: Proteomics
https://www.readbyqxmd.com/read/29321808/itraq-based-proteomic-analysis-to-identify-the-molecular-mechanism-of-zhibai-dihuang-granule-in-the-yin-deficiency-heat-syndrome-rats
#7
Chang-Ming Liu, Jing Chen, Su Yang, Ting-Ting Jiang, Zhong-Liang Chen, Hui-Hui Tu, Lian-Gen Mao, Yu-Ting Hu, Lin Gan, Zhong-Jie Li, Ji-Cheng Li
Background: Zhibai Dihuang Granule (ZDG) is a traditional Chinese medicine which has been used to treat Yin-deficiency-heat (YDH) syndrome for thousands of years in China. However, little work has been conducted to explore the molecular mechanism of ZDG in YDH syndrome, and the processes of YDH syndrome prevention and treatment have been developed slowly. The present study was aimed to explore the therapeutic mechanism of ZDG on YDH syndrome. Methods: The YDH syndrome rats were induced by hot Chinese herbs, then treated by ZDG orally for 1 week...
2018: Chinese Medicine
https://www.readbyqxmd.com/read/29321553/prediction-of-co-expression-genes-and-integrative-analysis-of-gene-microarray-and-proteomics-profile-of-keshan-disease
#8
Sen Wang, Rui Yan, Bin Wang, Peiru Du, Wuhong Tan, Mikko J Lammi, Xiong Guo
Keshan disease (KD) is a kind of endemic cardiomyopathy which has a high mortality. However, molecular mechanism in the pathogenesis of KD remains poorly understood. Serum samples were collected from 112 KD patients and 112 normal controls. Gene microarray was used to screen differently expressed genes. Genevestigator was applied to forecast co-expression genes of significant gene. iTRAQ proteomics analysis was used to verify significant genes and their co-expression genes. GO, COG, IPA and STRING were applied to undertake function categorization, pathway and network analysis separately...
January 10, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29317309/immune-related-proteins-detected-through-itraq-based-proteomics-analysis-of-intestines-from-apostichopus-japonicus-in-response-to-tussah-immunoreactive-substances
#9
Rui Mi, Yongxin Sun, Jianguang Li, Shuhui Ma, Zhixin Wen, Xuejun Li, Nan Meng, Yajie Li, Xingfan Du, Shuying Li
Apostichopus japonicus is a species of sea cucumber that is extensively bred as a marine delicacy because of its high nutritive and medicinal value. Immunostimulants are usually used to enhance the immunity of sea cucumber against diseases, but the physiological function of immunostimulants is poorly understood. In this study, we fed A. japonicus individuals with a diet supplemented with different concentrations of tussah immunoreactive substances (TIS), and then subjected their intestines to iTRAQ-based proteomic analysis...
January 6, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29315472/early-prognostic-factors-in-septic-shock-cancer-patients-a-prospective-study-with-a-proteomic-approach
#10
D Mokart, C Saillard, C Zemmour, M Bisbal, A Sannini, L Chow-Chine, J-P Brun, M Faucher, J-M Boher, Y Toiron, C Chabannon, J-P Borg, A Gonçalves, L Camoin
BACKGROUND: Organ failures are the main prognostic factors in septic shock. The aim was to assess classical clinico-biological parameters evaluating organ dysfunctions at intensive care unit admission, combined with proteomics, on day-30 mortality in critically ill onco-hematology patients admitted to the intensive care unit for septic shock. METHODS: This was a prospective monocenter cohort study. Clinico-biological parameters were collected at admission. Plasma proteomics analyses were performed, including protein profiling using isobaric Tag for Relative and Absolute Quantification (iTRAQ) and subsequent validation by ELISA...
January 7, 2018: Acta Anaesthesiologica Scandinavica
https://www.readbyqxmd.com/read/29311761/plasma-vascular-non-inflammatory-molecule-3-is-associated-with-gastrointestinal-acute-graft-versus-host-disease-in-mice
#11
Na Wang, Xiaoyi Qin, Yigeng Cao, Bin Liang, Kang Yu, Haige Ye
Background: Gastrointestinal acute graft-versus-host disease (GI aGVHD) is a lethal complication following allogeneic hematopoietic stem cell transplantation (HSCT). However, it is still very difficult to make a diagnosis of GI aGVHD in practice. To date, no consensus plasma biomarker of GI aGVHD can be used to help make a diagnosis. Here, we attempted to identify GI aGVHD associated plasma proteins in murine model, which can help make a diagnosis of GI aGVHD. Methods: We used 8-plex isobaric tags for relative and absolute quantitation (8-plex iTRAQ) to screen out proteins in plasma samples taken from murine models before and after allogeneic HSCT...
2018: Journal of Inflammation
https://www.readbyqxmd.com/read/29310583/proteomics-reveals-changes-in-hepatic-proteins-during-chicken-embryonic-development-an-alternative-model-to-study-human-obesity
#12
Mengling Peng, Shengnan Li, Qianian He, Jinlong Zhao, Longlong Li, Haitian Ma
BACKGROUND: Chicken embryos are widely used as a model for studies of obesity; however, no detailed information is available about the dynamic changes of proteins during the regulation of adipose biology and metabolism. Thus, the present study used an isobaric tags for relative and absolute quantitation (iTRAQ)-based proteomic approach to identify the changes in protein abundance at different stages of chicken embryonic development. RESULTS: In this study, the abundances of 293 hepatic proteins in 19-day old of chicken embryos compared with 14-day old and 160 hepatic proteins at hatching compared with 19-day old embryos were significantly changed...
January 8, 2018: BMC Genomics
https://www.readbyqxmd.com/read/29301952/the-deubiquitinase-otu5-regulates-root-responses-to-phosphate-starvation
#13
Der-Fen Suen, Yi-Hsiu Tsai, Ya-Tan Cheng, Ramalingam Radjacommare, Ram Nivas Ahirwar, Hongyong Fu, Wolfgang Schmidt
Phosphorus, taken up by plants as inorganic phosphate (Pi), is an essential but often growth-limiting mineral nutrient for plants. As part of an orchestrated response to improve its acquisition, insufficient Pi supply triggers alterations in root architecture and epidermal cell morphogenesis. Mutants defective in the expression of the OVARIAN TUMOR DOMAIN-CONTAINING DEUBIQUITINATING ENZYME5 (OTU5) exhibited a constitutive Pi deficiency root phenotype, comprising the formation of long and dense root hairs and attenuated primary root growth...
January 4, 2018: Plant Physiology
https://www.readbyqxmd.com/read/29300489/itraq-based-proteomic-analysis-reveals-recovery-of-impaired-mitochondrial-function-in-ischemic-myocardium-by-shenmai-formula
#14
Yi Wang, Yu Zhao, Wei Jiang, Xiaoping Zhao, Guanwei Fan, Han Zhang, Peiqiang Shen, Jiangmin He, Xiaohui Fan
Shenmai formula (SM) is a traditional medicinal remedy for treating cardiovascular diseases in China since 800 years ago, however its mechanism of action remains unclear. To explore the mechanism underlying cardioprotective effects of SM, iTRAQ-based proteomic approach was applied to analyze protein of myocardium in rats with myocardial ischemic injury. Upon treatment with SM and its two major components Red ginseng (RG) and Radix Ophiopogonis (OP), 101 differentially expressed proteins were filtered from a total of 711 detected and annotated proteins...
January 4, 2018: Journal of Proteome Research
https://www.readbyqxmd.com/read/29291001/successful-identification-of-a-predictive-biomarker-for-lymph-node-metastasis-in-colorectal-cancer-using-a-proteomic-approach
#15
Koichiro Mori, Yuji Toiyama, Kohei Otake, Shozo Ide, Hiroki Imaoka, Masato Okigami, Yoshinaga Okugawa, Hiroyuki Fujikawa, Susumu Saigusa, Junichiro Hiro, Minako Kobayashi, Masaki Ohi, Koji Tanaka, Yasuhiro Inoue, Yuhko Kobayashi, Yasuhiko Mohri, Issei Kobayashi, Ajay Goel, Masato Kusunoki
Colorectal cancer (CRC)-associated mortality is primarily caused by lymph node (LN) and distant metastasis, highlighting the need for biomarkers that predict LN metastasis and facilitate better therapeutic strategies. We used an Isobaric Tags for Relative and Absolute Quantification (iTRAQ)-based comparative proteomics approach to identify novel biomarkers for predicting LN metastasis in CRC patients. We analyzed five paired samples of CRC with or without LN metastasis, adjacent normal mucosa, and normal colon mucosa, and differentially expressed proteins were identified and subsequently validated at the protein and/or mRNA levels by immunohistochemistry and qRT-PCR, respectively...
December 5, 2017: Oncotarget
https://www.readbyqxmd.com/read/29289898/ionomic-metabolomic-and-proteomic-analyses-reveal-molecular-mechanisms-of-root-adaption-to-salt-stress-in-tibetan-wild-barley
#16
Qiufang Shen, Jiahua Yu, Liangbo Fu, Liyuan Wu, Fei Dai, Lixi Jiang, Dezhi Wu, Guoping Zhang
In our previous study, Tibetan wild barley (Hordeum spontaneum L.) has been found to be rich in the elite accessions with strong abiotic stress tolerance, including salt stress tolerance. However, the molecular mechanism of salt tolerance underlying the wild barley remains to be elucidated. In this study, two Tibetan wild barley accessions, XZ26 (salt-tolerant) and XZ169 (salt-sensitive), were used to investigate ionomic, metabolomic and proteomic responses in roots when exposed to 0, 200 (moderate) and 400 mM (high) salinity...
December 24, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/29287759/comparative-proteomics-analysis-of-trichinella-spiralis-muscle-larvae-exposure-to-albendazole-sulfoxide-stress
#17
Ruo Yu Peng, Hui Jun Ren, Chun Li Zhang, Pin Lv, Gao Hui Wei, Zhong Quan Wang, Jing Cui, Liang Ming
The drug albendazole (ABZ) has a positive effect against Trichinella spiralis infection and has been used for the treatment and prevention of trichinellosis in both humans and animals. However, the molecular mechanism of ABZ's effects on T. spiralis remains unknown now. Albendazole sulfoxide (ABZSO) is the main intermediary metabolic product of ABZ, and often used as a substitute of ABZ for the metabolism and bioavailability researches. Herein, isobaric tagging reagents for relative and absolute quantification (iTRAQ)-based LC-MS/MS analysis was used to identify the effect of ABZSO on the proteome of T...
December 26, 2017: Acta Tropica
https://www.readbyqxmd.com/read/29285398/combination-of-itraq-proteomics-and-rna-seq-transcriptomics-reveals-multiple-levels-of-regulation-in-phytoplasma-infected-ziziphus-jujuba-mill
#18
Xia Ye, Huiyu Wang, Peng Chen, Bing Fu, Mengyang Zhang, Jidong Li, Xianbo Zheng, Bin Tan, Jiancan Feng
Jujube witches' broom (JWB) is caused by infection with a phytoplasma. A multi-omics approach was taken during graft infection of jujube by JWB-infected scion through the analysis of the plant transcriptome, proteome and phytohormone levels. A high number of differentially expressed genes (DEGs) were identified 37 weeks after grafting (WAG), followed by observation of typical symptoms of JWB at 48 WAG. At 37 WAG, the majority of the upregulated DEGs and differentially expressed proteins (DEPs) were related to flavonoid biosynthesis, phenylalanine metabolism and phenylpropanoid biosynthesis...
2017: Horticulture Research
https://www.readbyqxmd.com/read/29285290/preliminary-quantitative-proteomics-analysis-in-chronic-and-latent-keshan-disease-by-itraq-labeling-approach
#19
Yuxiao Sun, Chuanyu Gao, Xianqing Wang, Yuhao Liu
Keshan disease is a congestive cardiomyopathy. Dietary selenium deficiency combined with additional stressors are recognized to cause the cardiomyopathies. In this study, clinical condition of individuals with different subtypes including chronic and latent were analyzed. ECG abnormalities, chest radiography, echocardiography and blood selenium concentration were assessed. Subsequently, in effort to uncover proteins that were reliably changed in patients, isobaric tags for absolute and relative quantitation technology was applied...
December 1, 2017: Oncotarget
https://www.readbyqxmd.com/read/29284148/itraq-based-comparative-proteome-analysis-for-molecular-mechanism-of-defense-against-acute-ammonia-toxicity-in-pacific-white-shrimp-litopenaeus-vannamei
#20
Xia Lu, Sheng Luan, Ping Dai, Xianhong Meng, Baoxiang Cao, Kun Luo, Jie Kong
In the practical farming of Litopenaeus vannamei, the intensive culture system and environmental pollution usually results in a high concentration of ammonia, which brings large detrimental effects to shrimp, such as increasing the susceptibility to pathogens and even causing high mortality. We have revealed that the survival time under acute ammonia stress varied substantially among different families and obtained ammonia-tolerant (LV_T) and ammonia-sensitive (LV_S) families. In order to understand the molecular mechanism of defense against ammonia toxicity in shrimp, we performed iTRAQ LC-MS/MS proteomic analysis between LV_T and LV_S groups of L...
December 25, 2017: Fish & Shellfish Immunology
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