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https://www.readbyqxmd.com/read/28728340/screening-of-differential-proteins-of-placenta-tissues-in-patients-with-pre-eclampsia-by-itraq-proteomics-techniques
#1
Wei-Hong Qi, Ming-Yan Zheng, Chao Li, Lin Xu, Jin-E Xu
BACKGROUND: Isotopically labeled relative and absolute quantification (iTRAQ) were applied together with liquid chromatogram-tandem mass spectrometry (LC-MS/MS) technique to screen and differentiate normal tissues and differential proteins of placenta tissue in the pre-eclampsia group, and to seek for a pre-eclampsia biomarker. METHODS: Thirty patients with severe pre-eclampsia (pre-eclampsia group) and 30 patients with normal full-term pregnancy (control group), who were admitted by the Obstetrical Department of The Affiliated Hospital of Qingdao University from December 2014 to June 2015, were collected for this study...
October 2017: Minerva Medica
https://www.readbyqxmd.com/read/28723944/application-of-mass-spectrometry-to-elucidate-the-pathophysiology-of-encephalitozoon-cuniculi-infection-in-rabbits
#2
Guillaume Desoubeaux, Maria Del Carmen Piqueras, Ana Pantin, Sanjoy K Bhattacharya, Roman Peschke, Anja Joachim, Carolyn Cray
Encephalitozoon cuniculi is a microsporidian species which can induce subclinical to serious disease in mammals including rabbits, a definitive natural host. The pathophysiology of infection has not been comprehensively elucidated. In this exploratory study, we utilized two mass spectrometry approaches: first, the analysis of the humoral response by profiling the microsporidian antigens as revealed by Western blot screening, and second, implementing the iTRAQ®-labeling protocol to focus on the changes within the host proteome during infection...
2017: PloS One
https://www.readbyqxmd.com/read/28720800/itraq-based-quantitative-proteomic-analysis-reveals-alterations-in-the-metabolism-of-actinidia-arguta
#3
Miaomiao Lin, Jinbao Fang, Xiujuan Qi, Yukuo Li, Jinyong Chen, Leiming Sun, Yunpeng Zhong
Actinidia arguta 'Tianyuanhong' is a new kiwifruit variety with an all-red pericarp and pulp, in contrast to the all-green pulp of A. arguta 'Yongfengyihao'. Transcriptome profile analysis of fruit color has been reported, however, the metabolic mechanisms producing red flesh remain unknown, and it is unclear why the pulp of 'Tianyuanhong' is red rather than green. Herein, we identified differences between the proteomes of two A. arguta cultivars with different fruit color by using iTRAQ-based quantitative proteomic methods during the stage of color change...
July 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28717962/comparative-proteome-analysis-for-identification-of-differentially-abundant-proteins-in-sids
#4
Noha El-Kashef, Iva Gomes, Katja Mercer-Chalmers-Bender, Peter M Schneider, Markus A Rothschild, Martin Juebner
Sudden infant death syndrome (SIDS) remains one of the most common causes of post-neonatal infant mortality in developed countries. Its pathogenesis is still poorly understood. The goal of the present study was to characterize changes in the proteome of SIDS compared to age-matched controls in heart and medulla tissues as well as in blood samples using two complementary quantitative proteomic techniques: 2D-DIGE and iTRAQ aiming to provide new insights into the mechanism of SIDS and to find diagnostic protein patterns...
July 17, 2017: International Journal of Legal Medicine
https://www.readbyqxmd.com/read/28711943/pancreatic-cancer-derived-exosomes-promote-tumor-metastasis-and-liver-pre-metastatic-niche-formation
#5
Zeqian Yu, Susu Zhao, Long Ren, Lishan Wang, Zhangjun Chen, Robert M Hoffman, Jiahua Zhou
Exosomes play important roles in cell-cell communication, and are likely mediators of the metastatic cascade in cancer. This study examined the role of exosomes in pancreatic cancer cell adhesion, migration, and invasion. We isolated and purified exosomes from two isogenic pancreatic cancer cell lines with different metastatic potentials. Uptake of exosomes from highly metastatic Panc02-H7 cells decreased adhesion and increased migration and invasion capacity in weakly metastatic Panc02 cells in vitro. Exosomes from highly metastatic pancreatic cancer cells induced liver pre-metastatic niche formation in naïve mice and promoted primary tumor growth and liver metastasis in vivo...
June 28, 2017: Oncotarget
https://www.readbyqxmd.com/read/28710486/comprehensive-transcriptomics-and-proteomics-analyses-of-pollinated-and-parthenocarpic-litchi-litchi-chinensis-sonn-fruits-during-early-development
#6
Wei Liu, Maoshan Chen, Lijun Bai, Zhenhua Zhuang, Chao Fan, Nonghui Jiang, Junsheng Zhao, Shuaipeng Ma, Xu Xiang
Litchi (Litchi chinensis Sonn.) is an important fruit that is widely cultivated in tropical and subtropical areas. In this study, we used RNA-Seq and iTRAQ technologies to compare the transcriptomes and proteomes of pollinated (polLFs) and parthenocarpic (parLFs) litchi fruits during early development (1 day, 2 days, 4 days and 6 days). We identified 4,864 DEGs in polLFs and 3,672 in parLFs, of which 2,835 were shared and 1,051 were specifically identified in parLFs. Compared to po1LFs, 768 DEGs were identified in parLFs...
July 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28708522/itraq-based-proteomic-profiling-of-granulosa-cells-from-lamb-and-ewe-after-superstimulation
#7
Jiapeng Lin, Yangsheng Wu, Bing Han, Ying Chen, Liqin Wang, Xiaolin Li, Mingjun Liu, Juncheng Huang
The number of oocytes obtained from lambs after FSH treatment is far greater than those acquired from adult ewes. However, these oocytes typically have reduced viability in comparison with adult ewe oocytes. However, the molecular mechanisms of differences in viability between lamb and ewe oocytes remain unknown. In the present research, we applied iTRAQ coupled with LC-MS/MS proteomic analysis in order to investigate the proteomic expression profile of granulosa cells from lambs and ewes following stimulation with FSH...
June 10, 2017: Theriogenology
https://www.readbyqxmd.com/read/28700355/identification-of-cd14-as-a-potential-biomarker-of-hepatocellular-carcinoma-using-itraq-quantitative-proteomics
#8
Jiao Guo, Rui Jing, Jian-Hong Zhong, Xin Dong, Yun-Xi Li, Yin-Kun Liu, Tian-Ren Huang, Chun-Yan Zhang
Hepatocellular carcinoma (HCC) is one of the most common malignant tumors without effective diagnostic biomarkers. This study intended to dynamically analyze serum proteomics in different pathological stages of liver diseases, and discover potential diagnostic biomarkers for early HCC. Patients with hepatitis B virus (HBV) infection, liver cirrhosis (LC), or HCC together with healthy controls (HC) were enrolled. Proteins differentially expressed between groups were screened using isobaric tagging for relative and absolute quantitation (iTRAQ), and promising HCC biomarker candidates were subjected to bioinformatics analysis, including K-means clustering, gene ontology (GO) and string network analysis...
June 28, 2017: Oncotarget
https://www.readbyqxmd.com/read/28699285/itraq-based-proteomic-analysis-reveals-the-roles-of-progesterone-receptor-inflammatory-and-fibrosis-for-slow-transit-constipation
#9
Yuwei Li, Yongjun Yu, Shuyuan Li, Mingqing Zhang, Zhao Zhang, Yang Shi, Xipeng Zhang, Shiwu Zhang
BACKGROUND AND AIM: Progesterone receptor, inflammation, neurotransmitter expression, and fibrosis are involved in slow-transit constipation. The aim of the present study was to examine whether patients with slow-transit constipation have an overexpression of progesterone receptor and serotonin which may impair the fibrosis of muscularis propria in colorectal wall. METHODS: High resolution colon manometry was used to record the colorectal peristaltic contractions of the proximal ascending and sigmoid colon in patients...
July 11, 2017: Journal of Gastroenterology and Hepatology
https://www.readbyqxmd.com/read/28698006/identification-of-tradd-as-a-potential-biomarker-in-human-uterine-leiomyoma-through-itraq-based-proteomic-profiling
#10
Yong Liu, Dan Lu, Jie Sheng, Lijing Luo, Weiyuan Zhang
Recurrent and refractory leiomyoma of uterus is one of the most common diseases in women of reproductive age. Despite its benign nature, uterine leiomyoma has presented an extremely deleterious impact on public health. The etiology of uterine leiomyoma remains unclear and clinical management remains suboptimal, leaving radical hysterectomy the only effective approach. Delineating the molecular mechanism underlying the leiomyoma initiation and progression remains an unmet clinical need. To screen proteins that were differentially expressed in uterine leiomyoma versus normal myometrium, we examined proteomic profile by isobaric tag for relative and absolute quantitation (iTRAQ) labeling coupled with liquid chromatography - tandem mass spectrometry (LC-MS/MS)...
July 8, 2017: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/28695418/csf-based-analysis-for-identification-of-potential-serum-biomarkers-of-neural-tube-defects
#11
Xinyu Yan, Lixin Mai, Changchun Lin, Wenji He, Gengsheng Yin, Jiakang Yu, Lian Huang, Sanqiang Pan
The protein composition of cerebrospinal fluid (CSF) in neural tube defects (NTDs) remains unknown. We investigated the protein composition of CSF from 9 infants with NTDs using isobaric tags for relative and absolute quantitation (iTRAQ). We identified 568 proteins in the CSF of infants with spina bifida, which is the most common type of NTD. Among these, 18 proteins were associated with neural tube closure in the CSF during human embryonic neurulation and 5 were involved in NTDs. Based on these results, an animal model was further utilized to investigate early serum biomarkers for NTDs...
July 10, 2017: Neuroscience Bulletin
https://www.readbyqxmd.com/read/28693420/differential-proteomic-analysis-of-replanted-rehmannia-glutinosa-roots-by-itraq-reveals-molecular-mechanisms-for-formation-of-replant-disease
#12
Mingjie Li, Yanhui Yang, Fajie Feng, Bao Zhang, Shuqiang Chen, Chuyun Yang, Li Gu, Fengqing Wang, Junyi Zhang, Aiguo Chen, Wenxiong Lin, Xinjian Chen, Zhongyi Zhang
BACKGROUND: The normal growth of Rehmannia glutinosa, a widely used medicinal plant in China, is severely disturbed by replant disease. The formation of replant disease commonly involves interactions among plants, allelochemicals and microbes; however, these relationships remain largely unclear. As a result, no effective measures are currently available to treat replant disease. RESULTS: In this study, an integrated R. glutinosa transcriptome was constructed, from which an R...
July 10, 2017: BMC Plant Biology
https://www.readbyqxmd.com/read/28690927/quantitative-proteome-level-analysis-of-paulownia-witches-broom-disease-with-methyl-methane-sulfonate-assistance-reveals-diverse-metabolic-changes-during-the-infection-and-recovery-processes
#13
Zhe Wang, Wenshan Liu, Guoqiang Fan, Xiaoqiao Zhai, Zhenli Zhao, Yanpeng Dong, Minjie Deng, Yabing Cao
Paulownia witches' broom (PaWB) disease caused by phytoplasma is a fatal disease that leads to considerable economic losses. Although there are a few reports describing studies of PaWB pathogenesis, the molecular mechanisms underlying phytoplasma pathogenicity in Paulownia trees remain uncharacterized. In this study, after building a transcriptome database containing 67,177 sequences, we used isobaric tags for relative and absolute quantification (iTRAQ) to quantify and analyze the proteome-level changes among healthy P...
2017: PeerJ
https://www.readbyqxmd.com/read/28683551/proteomic-analysis-of-differences-in-fiber-development-between-wild-and-cultivated-gossypium-hirsutum-l
#14
Yuan Qin, Hengling Wei, Huiru Sun, Pengbo Hao, Hantao Wang, Junji Su, Shuxun Yu
Upland cotton (Gossypium hirsutum L.) is one of the world's most important fiber crops, accounting for more than 90% of all cotton production. While their wild progenitors have relatively short and coarse, often tan-colored fibers, modern cotton cultivars possess longer, finer, stronger, and whiter fiber. In this study, the wild and cultivated cottons (YU-3 and TM-1) selected show significant differences on fibers at 10 day post-anthesis (DPA), 20 DPA and mature stages at the morphological level. In order to explore the effects of domestication, reveal molecular mechanisms underlying these phenotypic differences and better inform our efforts to further enhance cotton fiber quality, an iTRAQ-facilitated proteomic methods were performed on developing fibers...
July 7, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28679110/itraq-based-quantitative-proteomic-analysis-reveals-multiple-effects-of-emodin-to-haemophilus-parasuis
#15
Li Li, Ye Tian, Jiankang Yu, Xu Song, Renyong Jia, Qiankun Cui, Wenzhi Tong, Yuanfeng Zou, Lixia Li, Lizi Yin, Xiaoxia Liang, Changliang He, Guizhou Yue, Gang Ye, Ling Zhao, Fei Shi, Cheng Lv, Sanjie Cao, Zhongqiong Yin
Haemophilus parasuis, a symbiotic bacteria of upper respiratory tract of swine, is the etiological agent of Glässer's disease, which is characterized by fibrinous polyserositis. Emodin, exhibits antibacterial activity against H. parasuis, yet the action mode has not been fully understood. In present study, isobaric tag for relative and absolute quantification (iTRAQ) method was applied to analyze the global protein alteration of H. parasuis in response to 16μg/mL Emodin. In total, 338 proteins exhibiting significant differential expressions were identified...
July 2, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28679024/multi-pronged-proteomic-analysis-to-study-the-glioma-pathobiology-using-cerebrospinal-fluid-samples
#16
Nikita Gahoi, Darpan Malhotra, Aliasgar Moiyadi, Santosh G Varma, Mayuri N Gandhi, Sanjeeva Srivastava
PURPOSE: Gliomas are one of the most aggressive and lethal brain tumors arising from neoplastic transformation of astrocytes and oligodendrocytes. In this study, we have carried out a comprehensive quantitative analysis of proteome level differences in cerebrospinal fluid (CSF) across different grades of gliomas for a better understanding of glioma pathobiology. EXPERIMENTAL DESIGN: Glioma patients were diagnosed by radiology and histochemistry- based analyses. Differential proteomic analysis of high (n = 12) and low (n = 8) grade gliomas, and control (n = 3) samples was performed by using two complementary quantitative proteomic approaches; 2D-DIGE and iTRAQ...
July 5, 2017: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/28676819/comparative-proteomic-analysis-of-flag-leaves-reveals-new-insight-into-wheat-heat-adaptation
#17
Yunze Lu, Ruiqiong Li, Ruochen Wang, Xiaoming Wang, Weijun Zheng, Qixin Sun, Shaoming Tong, Shaojun Dai, Shengbao Xu
Hexaploid wheat (Triticum aestivum L.) is an important food crop but it is vulnerable to heat. The heat-responsive proteome of wheat remains to be fully elucidated because of previous technical and genomic limitations, and this has hindered our understanding of the mechanisms of wheat heat adaptation and advances in improving thermotolerance. Here, flag leaves of wheat during grain filling stage were subjected to high daytime temperature stress, and 258 heat-responsive proteins (HRPs) were identified with iTRAQ analysis...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28676664/itraq-based-quantitative-proteomic-analysis-of-the-inhibitory-effects-of-polysaccharides-from-viscum-coloratum-kom-nakai-on-hepg2-cells
#18
Yangyang Chai, Min Zhao
Viscum coloratum (Kom.) Nakai is one of active medicinal plants, and its active components, especially polysaccharides, have been shown to exhibit bioactivity. In this study, we examined the effects of three polysaccharide fractions from Viscum coloratum (Kom.) Nakai on HepG2 cell growth in a dose-dependent manner by using a CCK-8 assay kit. Flow cytometry analysis showed that VCP2 treatment delayed the cell cycle in the G1 phase and induced apoptosis in HepG2 cells, a result possibly due to the increased expression of p21(Wafl/Cip1) and Cyclin D and the decreased expression of Cyclin E and CDK4...
July 4, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28676260/quantitative-itraq-based-secretome-analysis-reveals-species-specific-and-temporal-shifts-in-carbon-utilization-strategies-among-manganese-ii-oxidizing-ascomycete-fungi
#19
Carolyn A Zeiner, Samuel O Purvine, Erika M Zink, Ljiljana Paša-Tolić, Dominique L Chaput, Si Wu, Cara M Santelli, Colleen M Hansel
Fungi generate a wide range of extracellular hydrolytic and oxidative enzymes and reactive metabolites, collectively known as the secretome, that synergistically drive plant litter decomposition in the environment. While secretome studies of model organisms have greatly expanded our knowledge of these enzymes, few have extended secretome characterization to environmental isolates, particularly filamentous Ascomycetes, or directly compared temporal patterns of enzyme utilization among diverse species. Thus, the mechanisms of carbon (C) degradation by many ubiquitous soil fungi remain poorly understood...
July 1, 2017: Fungal Genetics and Biology: FG & B
https://www.readbyqxmd.com/read/28675930/proteomic-analysis-of-the-e3-ubiquitin-ligase-hakai-highlights-a-role-in-plasticity-of-the-cytoskeleton-dynamics-and-in-the-proteasome-system
#20
Andrea Diaz-Diaz, Alba Casas-Pais, Valentina Calamia, Raquel Castosa, Olaia Martinez-Iglesias, Daniel Roca-Lema, Isabel Santamarina, Manuel Valladares-Ayerbes, Lourdes Calvo, Venancio Chantada, Angélica Figueroa
Carcinoma, the most common type of cancer, arises from epithelial cells. The transition from adenoma to carcinoma is associated with the loss of E-cadherin and, in consequence, the disruption of cell-cell contacts. E-cadherin is a tumour suppressor and it is down-regulated during epithelial-to-mesenchymal transition (EMT), indeed its loss is a predictor of poor prognosis. Hakai is an E3 ubiquitin-ligase protein that mediates E-cadherin ubiquitination, endocytosis and finally degradation, leading the alterations of cell-cell contacts...
July 4, 2017: Journal of Proteome Research
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