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Oana Maria Dragostin, Sangram Keshari Samal, Mamoni Dash, Florentina Lupascu, Andreea Pânzariu, Cristina Tuchilus, Nicolae Ghetu, Mihai Danciu, Peter Dubruel, Dragos Pieptu, Cornelia Vasile, Rodica Tatia, Lenuta Profire
Chitosan is a non-toxic, biocompatible, biodegradable natural cationic polymer known for its low imunogenicity, antimicrobial, antioxidant effects and wound-healing activity. To improve its therapeutic potential, new chitosan-sulfonamide derivatives have been designed to develop new wound dressing biomaterials. The structural, morphological and physico-chemical properties of synthesized chitosan derivatives were analyzed by FT-IR, (1)H NMR spectroscopy, scanning electron microscopy, swelling ability and porosity...
May 5, 2016: Carbohydrate Polymers
Tatiana Coura Oliveira, Samuel Lopes Lima, Josefina Bressan
Several studies have associated the digestibility of proteins to its imunogenic potential. Though, it was objectified to evaluate the impact of the thermal processing with high and low temperatures on the proteins structure of three types of foods, by means of the digestibility in vitro and electroforesis en gel de poliacrilamida. The pasteurize was observed in such a way, firing 95 ºC during 15 minutes, how much freeze dried causes qualitative and quantitative modifications of constituent proteins of the food...
May 2013: Nutrición Hospitalaria: Organo Oficial de la Sociedad Española de Nutrición Parenteral y Enteral
Petr G Lokhov, Elena E Balashova
Immune-mediated damage to tumor vessels is a potential means of preventing solid tumor progression. Antiangiogenic cancer vaccines capable of inducing this kind of damage include formulations comprised of endothelial cell-specific antigens. Identification of antigens capable of eliciting efficient vaccination is difficult because the endothelial cell phenotype is affected by surrounding tissues, including angiogenic stimuli received from surrounding tumor cells. Therefore, phenotype endothelial cell variations (heterogeneity) were examined in the context of the development of an efficient vaccine using mass spectrometry-based cell surface profiling...
January 2013: Human Vaccines & Immunotherapeutics
Rajdeep Choudhury, Partha Das, Tripti De, Tapati Chakraborti
Visceral leishmaniasis caused by the intracellular parasite Leishmania donovani is a major public health problem in the developing world. The emergence of increasing number of L. donovani strains resistance to antimonial drugs recommended worldwide requires the intervention of effective vaccine strategy for treatment of VL. In the present study L. donovani culture derived, soluble, secretory serine protease (pSP) has been shown to be vaccine target of VL. Protection from VL could be achieved by the use of safer vaccine which generally requires an adjuvant for induction of strong Th1 response...
January 2013: Immunobiology
Victoria Gnazzo, Ignacio Cebrian, Cecilia Langellotti, José Chabalgoity, Claudia Mongini, Valeria Quattrocchi, Patricia Zamorano
A live system to release heterologous antigens using an attenuated Salmonella strain was developed. We transformed Salmonella typhimurium LVR03 (S. LVR03) with a recombinant pTECH2 vector encoding 0, 1, 2, and 4 tandem copies of an imunogenic peptide of bovine herpes virus-1 (BoHV-1) glycoprotein D (gD). The system used yielded peptides fused to the non-toxic C fragment of the tetanus toxin (TetC), which has been shown to have adjuvant properties. Inoculation of BALB/c mice with the transformed Salmonella strains gave rise to a mild self-limited infection, with primary replication of bacteria occurring in Peyer's patches, even when the bacteria was administered intranasally...
February 2012: Viral Immunology
Bangalore Jayakrishna Mahendra, Shampur Narayan Madhusudana, Gadey Sampath, Soma Subhra Datta, Doddabele Hanumanthaiah Ashwathnarayana, Gonibeedu Manjunath Venkatesh, Mysore Kalappa Sudarshan, Gangaboraiah Bilugumba, Manjula Shamanna
Rabies continues to be a major public health problem in India. Nearly 17 million people are getting exposed to this disease every year. Therefore the need for effective post-exposure prophylaxis with safe and potent modern rabies vaccines continues to exist. Purified Duck Embryo Vaccine (PDEV) was introduced in this country to meet the ever increasing need for modern rabies vaccines. In this study we have assessed the safety, imunogenicity and tolerance of an indigenously manufactured PDEV in people exposed to dog and other animal bites...
September 30, 2010: Human Vaccines
Caterina Hatzifoti, Andrew Bacon, Helen Marriott, Peter Laing, Andrew W Heath
BACKGROUND: Antibody against CD40 is effective in enhancing immune responses to vaccines when chemically conjugated to the vaccine antigen. Unfortunately the requirement for chemical conjugation presents some difficulties in vaccine production and quality control which are compounded when multivalent vaccines are required. We explore here an alternative to chemical conjugation, involving the co-encapsulation of CD40 antibody and antigens in liposomal vehicles. METHODOLOGY/PRINCIPAL FINDINGS: Anti-mouse CD40 mAb or isotype control mAb were co-entrapped individually in cationic liposomal vehicles with pneumococcal polysaccharides or diphtheria and tetanus toxoids...
June 4, 2008: PloS One
C H J Lamers, P van Elzakker, S C L Langeveld, S Sleijfer, J W Gratama
BACKGROUND: Adoptive transfer of autologous T cells that are gene-transduced to express Ag-specific receptors represents an experimental strategy to provide tumor-specific immunity to cancer patients. We studied this concept in patients with metastatic renal cell cancer (RCC) using retroviral transduction of T cells with a single-chain Ab-G250 chimeric receptor [scFv(G250)]. We describe the validation of our clinical protocol for gene transduction and expansion of human T lymphocytes...
2006: Cytotherapy
Eva B Schadeck, Maninder Sidhu, Michael A Egan, Siew-Yen Chong, Priscilla Piacente, Amjed Masood, Dorys Garcia-Hand, Sarah Cappello, Vidia Roopchand, Shakuntala Megati, Jorge Quiroz, Jean D Boyer, Barbara K Felber, George N Pavlakis, David B Weiner, John H Eldridge, Zimra R Israel
An experimental pDNA vaccine adjuvant expressing IL-12 was evaluated for its ability to augment the humoral and cellular immune responses elicited by a SIVmac239 gag p39 expressing pDNA vaccine. To determine the effect of vaccine dose on the immune response, rhesus macaques were immunized with 1.5 mg or 5.0 mg of SIVmac239 gag pDNA, with or without co-immunization of IL-12 pDNA at 1.5 mg and 5.0 mg, respectively. Serum antibody responses to simian immunodeficiency virus (SIV) gag were increased 10-fold (p=0...
May 22, 2006: Vaccine
Xia Tian, Hong Cai, Yu-xian Zhu
OBJECTIVE: To investigate the imunogenicity and protective efficacy of the divalent DNA vaccine encoding the antigens MPT83 and MPT64 of Mycobacterium tuberculosis. METHODS: Two genes of the M. tuberculosis, MPT83 and MPT64, were amplified and cloned into the vector pJW4303. The vector containing the fusion gene DNA-MM and pJW4303 blank vector were transfected into CO57 cells. The expression of DNA-MM in the supernatant was detected by Western blotting. Thirty-six C57BL/6 mice were divided into 3 equal groups to be injected subcutaneously with the vector containing the fusion gene DNA-MM, pJW4303 blank vector, or bacillus of Calmette-Guerin vaccine (BCG) once a week for 3 weeks...
June 1, 2005: Zhonghua Yi Xue za Zhi [Chinese medical journal]
Ji Youn Hong, Myung Ja Choi
A one-step fluorescence polarization immunoassay (FPIA) was developed to measure progesterone level using an immunocomplex single reagent (SR), a preequilibrated mixture of antibody and tracer. Several fluorescence-labeled progesterone tracers were synthesized using the combination of two progesterone derivatives, 11alpha-hemisuccinyloxyprogesterone (P-11HS) and progesterone-3-(O-carboxymethyl)oxime (P-3CMO), and three fluorescence labels, fluoresceinamine isomer I and II, and ethylenediamine fluoresceinthiocarbamyl (EDF)...
October 2002: Biological & Pharmaceutical Bulletin
Shu-Bing Qian, Shi-Shu Chen
AIM:To investigate the therapeutic potential of gamma interferon (IFN-alpha) genemodified human hepatocellular carcinoma (HCC) cells.METHODS:The IFN-alpha gene was introduced retrovirally into four HCC cell lines.Secreted IFN-alpha activity was assessed using bioassay. The expression of MHC molecules was detected by FACS.Tumorigenicity was analysed by tumor formation in nude mice.RESULTS:Four IFN-alpha gene transduced HCC cell lines secreted different amounts of IFN-alpha, as in the same case of five clones derived from one HCC cell line...
June 1998: World Journal of Gastroenterology: WJG
S J Russell
There are good theoretical arguments for exploring the use of replicating gene-transfer vectors for human cancer therapy. Such vectors should be derived from weakly pathogenic human viruses with initially broad tissue tropism. Coat protein engineering and promoter engineering might be used successfully to narrow the tropism of the vector, enhancing its ability to target tumour cells. Killing of uninfected 'bystander' tumour cells could be achieved through prodrug activation by a vector-encoded enzyme. Rapid elimination of infused vector particles by circulating antiviral antibody would limit access to tumour deposits after repeated administration, but might be circumvented by the use of infectious nucleic acid which is poorly imunogenic [64]...
1994: European Journal of Cancer
M B Fiddler, L D Hudson, J G White, R J Desnick
Seven methods of erythrocyte entrapment--six by hypotonic exchange loading and one by chlorpromazine-induced endocytosis--were evaluted for (1) efficiency of incorporation of beta-glucuronidase, inulin, and glucose; (2) presence of beta-glucuronidase on the erythrocyte surface, as detected by hemagglutination or complement-dependent lysis in the prsence of antibody to beta-glucuronidase; (3) in vitro leakage of entrapped markers; and (4) morphologic alterations by scanning electron microscopy. Dependent on the method of entrapment, the incorporation of markers ranged from 0...
August 1980: Journal of Laboratory and Clinical Medicine
D Koffler, V Agnello, R Winchester, H G Kunkel
Single-stranded DNA (SDNA) occurs in high incidence and in greatest concentration in the sera of patients with systemic lupus erythematosus (SLE), where levels as high as 250 mug/ml were observed. SDNA appears to be an imunogen for anti-SDNA antibodies and forms complexes in vivo of both anti-SDNA-SDNA and anti-NDNA-SDNA types, which apparently play a role in the pathogenesis of the glomerulonephritis found in patients with SLE, SDNA is also found in high incidence but at lower levels in the sera of patients with rheumatoid arthritis...
January 1973: Journal of Clinical Investigation
M Bayer, P Wenk
Consecutive injections of microfilariae of L. carinii and D. viteae were carried out in the homologous or heterologous sequence into the corresponding natural or into the experimental hosts. Microfilariae of D. viteae are imunogenic in Sigmodon specifically and crossreacting as well. However, in the natural host, Meriones, they are doubtlessly tolerogenic. The sojourn and level of microfilaraemia are prolonged resp. higher when living D. viteae-Mf are injected s.c. followed by an i.v. injection. However, freeze-killed microfilariae of D...
December 1988: Tropical Medicine and Parasitology
M G Castro, P R Lowenstein, P W Saphier, E A Linton, P J Lowry
We have expressed human pre-procorticotrophin-releasing hormone (pre-proCRH) as a fusion protein to beta-galactosidase in Escherichia coli. The chimeric fusion protein was found in insoluble bacterial inclusion bodies. The inclusion bodies were isolated, purified and solubilized, and used as imunogens in rabbits to raise antibodies against the neuropeptide moiety. The antibodies generated were characterized by immunoassays and immunocytochemical techniques. The immunoassay results showed that the recombinant pre-proCRH antibodies cross-reacted with the full-length CRH precursor and several cleavage products derived from it, i...
December 1990: Journal of Molecular Endocrinology
V Stefanov, M Stefanova, I Belimezov
Studied with the imunogenicity of Salmonella gallinarum-pullorum bacteria treated with 1, 10 and 20 MR gamma rays or killed with acetone. Bacterial cultures were irradiated with a LMB-gamma M device, having a 137Cs source, single doses being of a 3,252 R/min power Biozzi's clearance test was employed to determine the changes in the immunogenic properties of the irradiated bacteria. The results obtained with both the clearance test and the protection test spoke of a considerable drop in the immunogenic capacity of the irradiated bacteria, using rates of 10, and especially of 20 MR gamma rays as compared to those killed with acetone...
1977: Veterinarno-meditsinski Nauki
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