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Nuno Carinhas, Daniel A M Pais, Alexey Koshkin, Paulo Fernandes, Ana S Coroadinha, Manuel J T Carrondo, Paula M Alves, Ana P Teixeira
Canine adenovirus vector type 2 (CAV2) represents an alternative to human adenovirus vectors for certain gene therapy applications, particularly neurodegenerative diseases. However, more efficient production processes, assisted by a greater understanding of the effect of infection on producer cells, are required. Combining [1,2-(13)C]glucose and [U-(13)C]glutamine, we apply for the first time (13)C-Metabolic flux analysis ((13)C-MFA) to study E1-transformed Madin-Darby Canine Kidney (MDCK) cells metabolism during growth and CAV2 production...
March 23, 2016: Scientific Reports
Agnieszka Zwolak, Agnieszka Szuster-Ciesielska, Jadwiga Daniluk, Olga Słabczyńska, Martyna Kandefer-Szerszeń
INTRODUCTION AND AIMS: Toll-like receptor 4 and proinflammatory cytokines play a central role in the progression of nonalcoholic fatty liver disease. We investigated IL-1, IL-6 and TNFα production and toll-like receptor 4 in both--obese and lean patients with non-alcoholic fatty liver disease who met different sets of metabolic syndrome criteria and linked the results with the disease burden. MATERIALS AND METHODS: 95 subjects were divided into four groups depending on the following criteria: presence or absence of metabolic syndrome and/or non-alcoholic fatty liver disease, glucose tolerance (prediabetes or normoglycemia) and BMI value (obese or lean)...
2015: PloS One
James M Phang, Wei Liu, Chad Hancock
Recent research suggests that chromatin-modifying enzymes are metabolic sensors regulating gene expression. Epigenetics is linked to metabolomics in response to the cellular microenvironment. Specific metabolites involved in this sensing mechanism include S-adenosylmethionine, acetyl-CoA, alphaketoglutarate and NAD (+) . Although the core metabolic pathways involving glucose have been emphasized as the source of these metabolites, the reprogramming of pathways involving non-essential amino acids may also play an important role, especially in cancer...
March 2013: Epigenetics: Official Journal of the DNA Methylation Society
Rosalucia Mazzei, Carmine Ungaro, Girolamo Garreffa, Francesca Luisa Conforti, Antonella Mollo, Teresa Sprovieri, Pasquale Servillo, Vincenzo Blasi, Olivier Gallo, Antonio Cerasa, Pier Luigi Lanza, Aldo Quattrone
L-2-Hydroxyglutaric aciduria (L-2-HGA) is a neurometabolic disease characterized by the presence of elevated levels of 2-hydroxyglutaric acid in the plasma, cerebrospinal fluid and urine. Clinical features in this inherited condition consist of mental deterioration, ataxia and motor deficits with pyramidal and extrapyramidal symptoms and signs. L-2-HGA is caused by mutations in the L-2-HGDH gene which most probably encodes for a L-2-hydroxyglutarate dehydrogenase, a putative mitochondrial protein converting L-2-hydroxyglutarate to alphaketoglutarate...
February 2011: Neurological Sciences
M Jeevanandam, M R Ali, L Ramias, W R Schiller
New substrates of potential benefit to critically ill patients receiving traditional nutritional support have been suggested to meet organ or tissue specific needs. The addition of an anabolic stimulus during nutritional support therefore appears to be a reasonable adjunct to augment protein synthesis. The purpose of this investigation was to evaluate the efficacy of the neutral salt ornithine alphaketoglutarate (OKGA) as a dietary supplement to promote growth in young rats by enhancing protein metabolism. A group of 16 male Sprague-Dawley rats (150-170g) were housed in individual metabolic cages and after dark-light cycle adaptation were fed ad libitum an oral liquid diet for 7 days...
June 1991: Clinical Nutrition: Official Journal of the European Society of Parenteral and Enteral Nutrition
No abstract text is available yet for this article.
1959: Atti Della Società Italiana di Cardiologia
No abstract text is available yet for this article.
March 1960: Rivista di Neurologia
No abstract text is available yet for this article.
April 1958: Archivio Per le Scienze Mediche
C F Bernardes, M M Fagian, J R Meyer-Fernandes, R F Castilho, A E Vercesi
Suramin, a polysulfonated naphthylamine, caused a dose dependent inhibition of carbonyl cyanide p-(tri-fluoromethoxy)phenylhydrazone-stimulated respiration supported either by succinate or a cocktail of alphaketoglutarate, malate and isocitrate in isolated rat liver mitochondria. The half-maximum effect was obtained at 40 and 140 microM suramin for NADH- or FADH(2)-linked substrates, respectively. The respiration supported by N,N,N'N'-tetramethyl-p-phenylenediamine oxidation was unaffected by suramin (<or=500 microM)...
December 1, 2001: Toxicology
B Herranz Jordán, F Moreno Romero, J J Cardesa García, I Santos Hurtado, A Aparicio Palomino, F Requena Guerrero
Eighty-one healthy prepubertal children of short stature, between two and twelve years of age, were divided into four homogeneous groups. Each group was treated with a placebo for one year and for a second year with one of the following drugs (double blind): clonidine (CI), arginine asprate (AA), ornithine alphaketoglutare (OKG), or cyproheptadine (Cp). CI and OKG did not better the standard deviation of height. AA and Cp did, but to no greater extent than the placebo. The growth rate did not change in any group...
June 1993: Anales Españoles de Pediatría
J Morice
No abstract text is available yet for this article.
June 1969: Therapeutique (La Semaine des Hôpitaux)
A F Haerer
No abstract text is available yet for this article.
1972: Acta Neurologica Scandinavica
A Gougoux, P Vinay, L Zizian, A Tejedor, J Noel
The effect of acetazolamide (ACZ) on renal metabolism and ammoniagenesis was studied in the dog in vivo and in vitro. ACZ was administered to 10 dogs with normal acid-base status and five with chronic metabolic acidosis induced by NH4Cl. In both groups of dogs, the acute administration of ACZ markedly reduced the urinary excretion of ammonium (from 33 to 10 in normal dogs and from 100 to 23 mumoles/100 ml GFR in acidotic dogs) whereas its release into the renal vein was increased in a reciprocal fashion (from 69 to 95 in normal dogs and from 91 to 152 mumoles/100 ml GFR in acidotic dogs)...
June 1987: Kidney International
M Rengel-Aranda, A Gougoux, P Vinay, J M Lopez-Novoa
Valproate is an antiepileptic drug known to induce hyperammonemia in humans. This hyperammonemia might result from a reduced detoxification of ammonium in the liver and/or from an accelerated renal ammoniagenesis. Six dogs with normal acid-base equilibrium and eight dogs with chronic metabolic acidosis were infused with valproate directly into their left renal artery in order to obtain arterial concentrations around 3 to 4 mM. The arterial ammonium concentration rose only in chronically acidotic dogs, whereas the lactate concentration and the lactate/pyruvate ratio increased in both groups...
November 1988: Kidney International
A Gougoux, D Dansereau, N Zan, P Vinay, J Noël, C Manillier
The effect of pH on the activities of various enzymes along the ammoniagenic pathway was tested on dog kidney cortex homogenates in an attempt to identify the metabolic steps that could be directly influenced by a low pH in this species. The activity of alphaketoglutarate dehydrogenase was markedly stimulated by acidification of the medium that decreased abruptly the km for alphaketoglutarate. By contrast, succinyl CoA synthetase and malate dehydrogenase remained relatively insensitive to pH changes. The apparent km of malic enzyme for malate was markedly decreased by an acid medium...
October 1989: American Journal of Kidney Diseases: the Official Journal of the National Kidney Foundation
A K De, J Nagchaudhuri
The total number of 43 young boys and girls of varying age group between 17-22 years were studied. The levels of blood ketoacids e.g., pyruvate and alphaketoglutarate were estimated spectrophotometrically. The RQ was determined by open circuit method of calorimetry by using Douglas bag for 13 boys and 13 girls and the BMR was estimated by closed circuit method of calorimetry using Collin's metabolex. With the increase of age the RQ and pyruvate were found to increase till the age of 20 years for both the sexes...
October 1976: Indian Journal of Physiology and Pharmacology
R R Chaffee, L Salganicoff, R M Arine, R H Rochelle, E L Schultz
No abstract text is available yet for this article.
August 8, 1977: Biochemical and Biophysical Research Communications
G Gay, C Villaume, M J Beaufrand, J P Felber, G Debry
After summing up existing theories about the origins and development of functional hepatic encephalopathy, the authors report on the effects of six-hour intravenous infusions of ornithine alphaketoglutarate (60 g dissolved in 500 ml distilled water), administered to 10 patients with ethylic hepatic cirrhosis in conjunction with a normal protein intake (70 g/day). Arterial blood ammonemia, venous blood aminoacidemia and the insulin/glucagon ratio did not vary during or after infusion. This method of treatment therefore seems to meet the protein requirements of these undernourished patients...
July 1979: Biomedicine / [publiée Pour L'A.A.I.C.I.G.]
M Dessemond-Negroni, A M Pauli, J J Charbit, C Gauthier, G François
Thiamine deficiency in the chronic alcoholic would appear to have a triple origin: inadequate intake, absorption and utilisation. Its consequences are well known: peripheral neuropathy, WERNICKE and KORSAKOFF type encephalopathies and cardiac problems (with asystole at the extreme). The active principle of thiamine, TPP or cocarboxylase, is involved as a coenzyme of pyruvate decarboxylase and of alphaketoglutarate decarboxylase in the ocidative decarboxylation reactions of the Krebs cycle. TPP is involved as a coenzyme of transketolase in the transketolisation reactions of the pentose pathway...
1977: Annales de L'anesthésiologie Française
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