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Chil development

M M Vaezirad, M G Koene, J A Wagenaar, J P M van Putten
In ovo immunization of chicken embryos with live vaccines is an effective strategy to protect chickens against several viral pathogens. We investigated the immune response of chicken embryos to purified recombinant protein. In ovo delivery of Salmonella flagellin to 18-day old embryonated eggs resulted in elevated pro-inflammatory chIL-6 and chIL-8 (CXCL8-CXCLi2) cytokine transcript levels in the intestine but not in the spleen at 24 h post-injection. Analysis of the chicken Toll-like receptor (TLR) repertoire in 19-day old embryos revealed gene transcripts in intestinal and spleen tissue for most chicken TLRs, including TLR5 which recognizes Salmonella flagellin (FliC)...
April 12, 2018: Vaccine
Sun Young Noh, Kyungmin Jeong, Yong-Chil Seo, Chang-Hoi Kim, Jongwon Park, Yoo Rark Choi, Sung Uk Lee, Yeong-Geol Bae, Seungho Kim
This paper introduces a specialized robotic system under development for radiosurgery using a small-sized linear accelerator. The robotic system is a 5-DOF manipulator that can be installed above a patient to make an upper hemispherical workspace centered in a target point. In order to determine the optimal lengths of the link, we consider the requirements for the workspace of a linear accelerator for radiosurgery. A more suitable kinematic structure than conventional industrial manipulators is proposed, and the kinematic analysis is also provided...
2017: Journal of Healthcare Engineering
Jeong-A Seo, Yirang Im, Si Hyun Han, Chil Won Lee, Jun Yeob Lee
Unconventional blue thermally activated delayed fluorescent emitters having electron-donating type indolocarbazole as an acceptor were developed by attaching carbazolylcarbazole or acridine donors to the indolocarbazole acceptor. Three compounds were derived from the indolocarbazole acceptor. The indolocarbazole-acridine combined products showed efficient delayed fluorescent behavior and a high quantum efficiency of 19.5% with a color coordinate of (0.15, 0.16) when they were evaluated as thermally activated delayed fluorescent emitters in deep blue fluorescent devices...
October 17, 2017: ACS Applied Materials & Interfaces
Xiaoyu Guan, Zhichao Xu, Yongqiang Wang, Xiaoqi Li, Hong Cao, Shijun Zheng
To develop monoclonal antibodies (McAbs) against chicken interleukin 4 (chIL-4), we subcloned the mature chIL-4 gene into prokaryotic expression vectors pET-28a and pGEX-6P-1, then expressed and purified the recombinant proteins. We immunized BALB/c mice with the purified His-chIL-4 protein and fused the murine splenocytes with SP2/0 after 4 times of immunization. We used the GST-chIL-4 protein as a coating antigen to establish an indirect ELISA to screen positive clones. After screening and 3 rounds of cloning process, we obtained 3 hybridomas that stably secreted McAbs against chIL-4, and named 1G11-3B, 2E5-3D, and 1G11-5H...
January 25, 2017: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
Sungwon Kim, Myeongseon Park, Ariel E Leon, James S Adelman, Dana M Hawley, Rami A Dalloul
BACKGROUND: A unique clade of the bacterium Mycoplasma gallisepticum (MG), which causes chronic respiratory disease in poultry, has resulted in annual epidemics of conjunctivitis in North American house finches since the 1990s. Currently, few immunological tools have been validated for this songbird species. Interleukin-1β (IL-1β) is a prototypic multifunctional cytokine and can affect almost every cell type during Mycoplasma infection. The overall goal of this study was to develop and validate a direct ELISA assay for house finch IL-1β (HfIL-1β) using a cross-reactive chicken antibody...
August 30, 2017: BMC Veterinary Research
Ha-Na Yoo, Jin-Won Lee, Jeong-Chil Yoo
Bilateral symmetry is assumed to contribute to the evolution of eye color, with the left and right eye being the same color in most vertebrates; yet, few studies tested this assumption. Here, we compared the amount of iris flecking (black spots presented on the iris) between the left and right eye of 76 adult common cuckoos Cuculus canorus. We found considerable variation in the total amount of iris flecking among individuals, with variation being associated with body size and sex. We also found that the amount of iris flecking differed between the left and right eye and that this left-right asymmetry was not random, with the left eye almost always being darker than the right eye...
August 8, 2017: Scientific Reports
Amira Abdelrasoul, Huu Doan, Ali Lohi, Chil-Hung Cheng
The goal of the current research was to critically examine the role of the shape and the size of contaminated particles for an accurate prediction of membrane fouling phenomenon. Polycarbonate flat membranes (PC) with uniform pore sizes of 0.05 and 0.1 µm, in addition to Polysulfone membranes (PS) with molecular weight cut off (MWCO) of 60,000 kDa were used under a constant feed flow rate and a cross-flow mode in ultrafiltration of a latex paint solution featuring a wide range of particle size distribution...
January 2018: Environmental Technology
Seon Chil Kim, Jeong Ryeol Choi, Byeong Kyou Jeon
The purpose of this paper is to develop a lightweight apron that will be used for shielding low intensity radiation in medical imaging radiography room and to apply it to a custom-made effective shielding. The quality of existing aprons made for protecting our bodies from direct radiation are improved so that they are suitable for scattered X-rays. Textiles that prevent bodies from radiation are made by combining barium sulfate and liquid silicon. These materials have the function of shielding radiation in a manner like lead...
2016: Scientific Reports
Min Jeong Kim, U Suk Jung, Seung Woo Jeon, Jae Sung Lee, Won Seob Kim, Sang Bum Lee, Youn Chil Kim, Bae Young Kim, Tao Wang, Hong Gu Lee
This study was conducted to examine the effects of adding phytoncide oil extracted from Korean pine nut cone byproduct to the diet of dairy cows on milk yield and compositions, fatty acid characteristics, complete blood count and stress response. A total of 74 Holstein cows were used for 30 days and divided into two groups. Each group was given a basal diet (C) or an experimental diet containing phytoncide additives at 0.016% (T) in feed. The results showed that phytoncide feeding had no effect on milk yield...
December 2016: Asian-Australasian Journal of Animal Sciences
Masaaki Nakamura, Shiun-Jr Yang, Po-Yu Tsai, Toshio Kasai, King-Chuen Lin, Dock-Chil Che, Andrea Lombardi, Federico Palazzetti, Vincenzo Aquilanti
Molecular orientation is a fundamental requisite in the study of stereodirected dynamics of collisional and photoinitiated processes. In this past decade, variable hexapolar electric filters have been developed and employed for the rotational-state selection and the alignment of molecules of increasing complexity, for which the main difficulties are their mass, their low symmetry, and the very dense rotational manifold. In this work, for the first time, a complex molecule such as 2-bromobutane, an asymmetric top containing a heavy atom (the bromine), was successfully oriented by a weak homogeneous field placed downstream from the hexapolar filter...
July 14, 2016: Journal of Physical Chemistry. A
Zhiguang Wu, Tuanjun Hu, Lisa Rothwell, Lonneke Vervelde, Pete Kaiser, Kay Boulton, Matthew J Nolan, Fiona M Tomley, Damer P Blake, David A Hume
In mammals, the inducible cytokine interleukin 10 is a feedback negative regulator of inflammation. To determine the extent to which this function is conserved in birds, recombinant chicken IL-10 was expressed as a secreted human Ig Fc fusion protein (chIL-10-Fc) and used to immunise mice. Five monoclonal antibodies (mAb) which specifically recognise chicken IL-10 were generated and characterised. Two capture ELISA assays were developed which detected native chIL-10 secreted from chicken bone marrow-derived macrophages (chBMMs) stimulated with lipopolysaccharide (LPS)...
October 2016: Developmental and Comparative Immunology
Sun Min Lim, Byeong Bae Park, Keun-Chil Park, Hoon-Kyo Kim, Jong Seok Lee, Sung Hwa Bae, Seung-Sei Lee, Jin-Hyoung Kang, Se-Hoon Park, Gyeong-Won Lee, Hyo-Rac Lee, Jae Hong Seo, Yong Tae Kim, Sung Hyun Yang, Joo-Hang Kim
BACKGROUND: To investigate the efficacy, safety, and tolerability of weekly docetaxel treatment in advanced non-small cell lung cancer (NSCLC) patients in Korea. METHODS: This prospective observational study included Korean advanced NSCLC patients with Eastern Cooperative Oncology Group performance status <2 who received weekly monotherapy of docetaxel at a dose determined by the physician. Efficacy measurements included tumor response rate, overall survival (OS), progression-free survival, and one-year survival rate...
March 2016: Thoracic Cancer
Zhanjun Yang, Mimi Lu, Juan Li, Zining Tan, Hua Dai, Xin'an Jiao, Xiaoya Hu
Chicken interleukin-4 (ChIL-4), which is released by activated type 2 helper (Th2) cells following their stimulation in vitro, is an important indicator for the study of cell-mediated immunity in chickens after infection or vaccination. In this work, the first ChIL-4 chemiluminescent (CL) immunosensor was developed via the immobilization of monoclonal ChIL-4 antibodies on a nitrogen-doped graphene (NG)-chitosan nanocomposite matrix. NG nanosheets were used for the first time in the CL immunoassay to provide a biocompatible microenvironment for the immobilized capture antibody...
March 15, 2017: Biosensors & Bioelectronics
Jaehwan Kim, Chil-Hwan Oh, Jiehyun Jeon, Yoosang Baek, Jaewoo Ahn, Dong Joo Kim, Hyun-Soo Lee, Joel Correa da Rosa, Mayte Suárez-Fariñas, Michelle A Lowes, James G Krueger
Psoriasis is present in all racial groups, but in varying frequencies and severity. Considering that small plaque psoriasis is specific to the Asian population and severe psoriasis is more predominant in the Western population, we defined Asian small and intermediate plaque psoriasis as psoriasis subtypes and compared their molecular signatures with the classic subtype of Western large plaque psoriasis. Two different characteristics of psoriatic spreading-vertical growth and radial expansion-were contrasted between subtypes, and genomic data were correlated to histologic and clinical measurements...
January 2016: Journal of Investigative Dermatology
Ping Tou Gee, Chen Yee Liew, Meng Chil Thong, Melvin C L Gay
We have developed a method for analysing vitamin E using ultra-performance convergence chromatography with a chromatographic runtime of 5.5 min. A well-resolved chromatogram with excellent precision in retention time revealed seven vitamin E components in the palm oil derived tocotrienol-rich fraction. The major vitamin E components were α-tocopherol, α-tocotrienol, γ-tocotrienol and δ-tocotrienol whereas the minor vitamin E components were α-tocomonoenol, β-tocotrienol and an unreported trace component...
April 1, 2016: Food Chemistry
Jaehwan Kim, Chil-Hwan Oh, Jiehyun Jeon, Yoosang Baek, Jaewoo Ahn, Dong Joo Kim, Hyun-Soo Lee, Joel Correa da Rosa, Mayte Suárez-Fariñas, Michelle A Lowes, James G Krueger
Psoriasis is present in all racial groups, but in varying frequencies and severity. Considering that small plaque psoriasis is specific to the Asian population and severe psoriasis is more predominant in the Western population, we defined Asian small and intermediate plaque psoriasis as psoriasis subtypes, and compared their molecular signatures with classic subtype of Western large plaque psoriasis. Two different characteristics of psoriatic spreading-vertical growth and radial expansion-were contrasted between subtypes, and genomic data were correlated to histologic and clinical measurements...
September 29, 2015: Journal of Investigative Dermatology
Marc Fiedler, Michael Graeb, Juliusz Mieszczanek, Trevor J Rutherford, Christopher M Johnson, Mariann Bienz
TCF/LEF factors are ancient context-dependent enhancer-binding proteins that are activated by β-catenin following Wnt signaling. They control embryonic development and adult stem cell compartments, and their dysregulation often causes cancer. β-catenin-dependent transcription relies on the NPF motif of Pygo proteins. Here, we use a proteomics approach to discover the Chip/LDB-SSDP (ChiLS) complex as the ligand specifically binding to NPF. ChiLS also recognizes NPF motifs in other nuclear factors including Runt/RUNX2 and Drosophila ARID1, and binds to Groucho/TLE...
August 27, 2015: ELife
Carlos M S Dutok, Clara Azalea Berenguer-Rivas, Elizabeth Rodríguez-Leblanch, Liliana Pérez-Jackson, Idelsy Chil-Nuñez, Julio César Escalona-Arranz, Bernardo Reyes-Tur, Margareth M C Queiroz
The common use of Pouteria mammosa (L.) Cronquist, "Mamey or Zapote," in food and ethnobotanic medicine shows its low or absent toxicity as fruit extracts prepared from seeds. However, it is essential to conduct security trials to scientifically support their use in drug therapy. This study evaluated the aqueous and hydroalcoholic extract (25%) Acute Oral Toxicity, obtained from the seeds of P. mammosa, in Sprague Dawley rats and dermal and eye irritability in New Zealand rabbits. The 404 and 405 acute dermal and eye irritation/corrosion guidelines were used, as well as the 423 Acute Oral Toxicity guideline, Acute Toxic Class Method of the Organization for Economic Cooperation and Development (OECD)...
2015: TheScientificWorldJournal
Andrzej Nowakowski, Marek Cybulski, Andrzej Śliwczyński, Arkadiusz Chil, Zbigniew Teter, Przemysław Seroczyński, Marc Arbyn, Ahti Anttila
BACKGROUND: Well-organised quality-controlled screening can substantially reduce the burden of cervical cancer (CC). European guidelines (EuG) for quality assurance in CC screening provide guidance on all aspects of an organised screening programme. Organised CC screening in Poland was introduced in 2007. The purpose of our study was to analyse: (i) adherence of the programme to EuG; (ii) programme process and performance indicators; (iii) impact of the programme on the incidence of and mortality from CC...
2015: BMC Cancer
Yi Zhang, Chil-Woo Lee, Nora Wehner, Fabian Imdahl, Veselova Svetlana, Christoph Weiste, Wolfgang Dröge-Laser, Rosalia Deeken
Virulent Agrobacterium tumefaciens strains integrate their T-DNA into the plant genome where the encoded agrobacterial oncogenes are expressed and cause crown gall disease. Essential for crown gall development are IaaH (indole-3-acetamide hydrolase), IaaM (tryptophan monooxygenase) and Ipt (isopentenyl transferase), which encode enzymes for the biosynthesis of auxin (IaaH, IaaM) and cytokinin (Ipt). Although these oncogenes are well studied as the tumor-inducing principle, nothing is known about the regulation of oncogene expression in plant cells...
January 2015: PLoS Pathogens
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