Caitlin Peaslee, Cristina Esteva-Font, Tao Su, Antonio Munoz-Howell, Caroline Duwaerts, Zhe Liu, Sneha Rao, Ke Liu, Marisa Medina, Julie B Sneddon, Jacquelyn J Maher, Aras N Mattis
Induced pluripotent stem cells (iPSCs) provide an important tool for the generation of patient-derived cells, including hepatocyte-like cells, via developmental cues through an endoderm intermediate. However, most iPSC lines fail to differentiate into endoderm, with induction resulting in apoptosis. To address this issue, we built upon published methods to develop an improved protocol. We discovered that doxycycline dramatically enhances the efficiency of iPSC to endoderm differentiation by inhibiting apoptosis and promoting proliferation via the AKT (protein kinase B) pathway...
May 12, 2021: Hepatology: Official Journal of the American Association for the Study of Liver Diseases