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https://www.readbyqxmd.com/read/29657828/an-objective-in-vivo-diagnostic-method-for-inflammatory-bowel-disease
#1
Sophie C Payne, Robert K Shepherd, Alicia Sedo, James B Fallon, John B Furness
Inflammatory damage to the bowel, as occurs in inflammatory bowel disease (IBD), is debilitating to patients. In both patients and animal experimental models, histological analyses of biopsies and endoscopic examinations are used to evaluate the disease state. However, such measurements often have delays and are invasive, while endoscopy is not quantitatively objective. Therefore, a real-time quantitative method to assess compromised mucosal barrier function is advantageous. We investigated the correlation of in vivo changes in electrical transmural impedance with histological measures of inflammation...
March 2018: Royal Society Open Science
https://www.readbyqxmd.com/read/29617332/rapid-identification-of-intact-staphylococcal-bacteriophages-using-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry
#2
Dana Štveráková, Ondrej Šedo, Martin Benešík, Zbyněk Zdráhal, Jiří Doškař, Roman Pantůček
Staphylococcus aureus is a major causative agent of infections associated with hospital environments, where antibiotic-resistant strains have emerged as a significant threat. Phage therapy could offer a safe and effective alternative to antibiotics. Phage preparations should comply with quality and safety requirements; therefore, it is important to develop efficient production control technologies. This study was conducted to develop and evaluate a rapid and reliable method for identifying staphylococcal bacteriophages, based on detecting their specific proteins using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) profiling that is among the suggested methods for meeting the regulations of pharmaceutical authorities...
April 4, 2018: Viruses
https://www.readbyqxmd.com/read/29435761/fibroblasts-potentiate-melanoma-cells-in-vitro-invasiveness-induced-by-uv-irradiated-keratinocytes
#3
Njainday Pulo Jobe, Veronika Živicová, Alžběta Mifková, Daniel Rösel, Barbora Dvořánková, Ondřej Kodet, Hynek Strnad, Michal Kolář, Aleksi Šedo, Karel Smetana, Karolina Strnadová, Jan Brábek, Lukáš Lacina
Melanoma represents a malignant disease with steadily increasing incidence. UV-irradiation is a recognized key factor in melanoma initiation. Therefore, the efficient prevention of UV tissue damage bears a critical potential for melanoma prevention. In this study, we tested the effect of UV irradiation of normal keratinocytes and their consequent interaction with normal and cancer-associated fibroblasts isolated from melanoma, respectively. Using this model of UV influenced microenvironment, we measured melanoma cell migration in 3-D collagen gels...
February 12, 2018: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/29434344/identification-of-micrornas-differentially-expressed-in-glioblastoma-stem-like-cells-and-their-association-with-patient-survival
#4
Jiri Sana, Petr Busek, Pavel Fadrus, Andrej Besse, Lenka Radova, Marek Vecera, Stefan Reguli, Lucie Stollinova Sromova, Marek Hilser, Radim Lipina, Radek Lakomy, Leos Kren, Martin Smrcka, Aleksi Sedo, Ondrej Slaby
Glioblastoma stem-like cells (GSCs) are critical for the aggressiveness and progression of glioblastoma (GBM) and contribute to its resistance to adjuvant treatment. MicroRNAs (miRNAs) are small, non-coding RNAs controlling gene expression at the post-transcriptional level, which are known to be important regulators of the stem-like features. Moreover, miRNAs have been previously proved to be promising diagnostic biomarkers in several cancers including GBM. Using global expression analysis of miRNAs in 10 paired in-vitro as well as in-vivo characterized primary GSC and non-stem glioblastoma cultures, we identified a miRNA signature associated with the stem-like phenotype in GBM...
February 12, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29352504/trophic-niche-capture-efficiency-and-venom-profiles-of-six-sympatric-ant-eating-spider-species-araneae-zodariidae
#5
Stano Pekár, Lenka Petráková, Ondrej Šedo, Stanislav Korenko, Zbyněk Zdráhal
The arms race between specialist predators and their prey has resulted in the evolution of a variety of specific adaptations. In venomous predators, this can include venom composition, particularly if predators are specialized on dangerous prey. Here, we performed an integrative study using six species of highly specialized ant-eating spiders of the genus Zodarion to investigate their phylogeny, realized trophic niche, efficacy in the capture of various ant species and venom composition. Data on natural diet obtained by next-generation sequencing and field observations showed that the six Zodarion species exploit different ant species...
February 2018: Molecular Ecology
https://www.readbyqxmd.com/read/29116706/effect-of-sperm-dna-fragmentation-on-embryo-development-clinical-and-biological-aspects
#6
Cristian Alvarez Sedó, Melina Bilinski, Daniela Lorenzi, Heydy Uriondo, Felicitas Noblía, Valeria Longobucco, Estefanía Ventimiglia Lagar, Florencia Nodar
OBJECTIVE: The aim of this study was to investigate the effect of sperm DNA fragmentation on fertilization rate, embryo development (blastulation rate), and pregnancy outcomes for ICSI cycles performed in a cohort of couples using donor eggs and to assess the remaining embryos that were not transferred or frozen for apoptotic markers. METHODS: Eighty-two women (egg recipients) were included in the study (2016) were included in the study. The recipients' mean age was 41...
December 1, 2017: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/29039817/replacing-nitrogen-by-sulfur-from-structurally-disordered-eumelanins-to-regioregular-thiomelanin-polymers
#7
Mariagrazia Iacomino, Juan Mancebo-Aracil, Mireia Guardingo, Raquel Martín, Gerardino D'Errico, Marco Perfetti, Paola Manini, Orlando Crescenzi, Félix Busqué, Alessandra Napolitano, Marco d'Ischia, Josep Sedó, Daniel Ruiz-Molina
The oxidative polymerization of 5,6-dihydroxybenzothiophene (DHBT), the sulfur analog of the key eumelanin building block 5,6-dihydroxyindole (DHI), was investigated to probe the role of nitrogen in eumelanin build-up and properties. Unlike DHI, which gives a typical black insoluble eumelanin polymer on oxidation, DHBT is converted to a grayish amorphous solid (referred to as thiomelanin) with visible absorption and electron paramagnetic resonance properties different from those of DHI melanin. Mass spectrometry experiments revealed gradational mixtures of oligomers up to the decamer level...
October 17, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28960952/polydopamine-like-coatings-as-payload-gatekeepers-for-mesoporous-silica-nanoparticles
#8
Miguel-Ángel Moreno-Villaécija, Josep Sedó-Vegara, Eduardo Guisasola, Alejandro Baeza, María Vallet Regí, Fabiana Nador, Daniel Ruiz-Molina
We report the use of bis-catecholic polymers as candidates for obtaining effective, tunable gatekeeping coatings for mesoporous silica nanoparticles (MSNs) intended for drug release applications. In monomers, catechol rings act as adhesive moieties and reactive sites for polymerization, together with middle linkers which may be chosen to tune the physicochemical properties of the resulting coating. Stable and low-toxicity coatings (pNDGA and pBHZ) were prepared from two bis-catechols of different polarity (NDGA and BHZ) on MSN carriers previously loaded with rhodamine B (RhB) as a model payload, by means of a previously reported synthetic methodology and without any previous surface modification...
September 29, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28953383/inhibitor-decorated-polymer-conjugates-targeting-fibroblast-activation-protein
#9
Petra Dvořáková, Petr Bušek, Tomáš Knedlík, Jiří Schimer, Tomáš Etrych, Libor Kostka, Lucie Stollinová Šromová, Vladimír Šubr, Pavel Šácha, Aleksi Šedo, Jan Konvalinka
Proteases are directly involved in cancer pathogenesis. Expression of fibroblast activation protein (FAP) is upregulated in stromal fibroblasts in more than 90% of epithelial cancers and is associated with tumor progression. FAP expression is minimal or absent in most normal adult tissues, suggesting its promise as a target for the diagnosis or treatment of various cancers. Here, we report preparation of a polymer conjugate (an iBody) containing a FAP-specific inhibitor as the targeting ligand. The iBody inhibits both human and mouse FAP with low nanomolar inhibition constants but does not inhibit close FAP homologues dipeptidyl peptidase IV, dipeptidyl peptidase 9, and prolyl oligopeptidase...
October 26, 2017: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28711492/species-specific-real-time-rt-pcr-analysis-of-expression-of-stromal-cell-genes-in-a-tumor-xenotransplantation-model-in-mice
#10
Evzen Krepela, Petr Busek, Marek Hilser, Zdislava Vanickova, Aleksi Sedo
Human tumor xenografts in mice together with the species-specific analysis of expressed genes allow to study the molecular processes driving tumor growth and progression in vivo and help to develop and evaluate anticancer therapies. In the present work, we designed and validated species-specific real-time RT-PCR assays for discrimination and quantitation of expression of human and mouse transcripts in cancer and stromal cells including dipeptidyl peptidase (DPP) 4, DPP8, DPP9, fibroblast activation protein (FAP) and CXC chemokine receptor 4 in mixed human-mouse biological samples...
September 9, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28671519/acinetobacter-colistiniresistens-sp-nov-formerly-genomic-species-13-sensu-bouvet-and-jeanjean-and-genomic-species-14-sensu-tjernberg-and-ursing-isolated-from-human-infections-and-characterized-by-intrinsic-resistance-to-polymyxins
#11
Alexandr Nemec, Lenka Radolfova-Krizova, Martina Maixnerova, Ondrej Sedo
Strains of the genusAcinetobacter, classified as genomic species 13BJ/14TU have been previously associated with human infections and resistance to colistin. To clarify the taxonomy of this provisional group, we investigated 24 strains that have been isolated from humans since the 1960s in 10 countries. The genus-wide analysis of the rpoB and gyrB sequences of all strains and whole-genome sequences of strains representing different rpoB/gyrB genotypes showed that the 24 strains formed a distinct monophyletic group within the so-called haemolytic clade of the genus Acinetobacter...
July 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28471824/early-detection-of-sporadic-pancreatic-cancer-time-for-change
#12
Přemysl Frič, Aleksi Šedo, Jan Škrha, Petr Bušek, Martin Laclav, Pavel Škrha, Miroslav Zavoral
Sporadic pancreatic cancer amounts to ∼90% of all pancreatic cancers. It is a gloomy depressive disease and the most recalcitrant malignancy, with a very low 5-year survival (3-6%). At present, diagnostic methods are commonly applied, as used half a century ago, after the appearance of local and systemic symptoms (abdominal and back pain, cholestasis, painless jaundice, fatigue, anorexia, weight loss, anemia, peripheral phlebitis, and cachexia). Unfortunately, these symptoms are harbingers of an advanced disease...
August 2017: European Journal of Gastroenterology & Hepatology
https://www.readbyqxmd.com/read/28452380/heterogeneity-of-molecular-forms-of-dipeptidyl-peptidase-iv-and-fibroblast-activation-protein-in-human-glioblastomas
#13
Ivana Matrasova, Petr Busek, Eva Balaziova, Aleksi Sedo
BACKGROUND AND AIMS: Proteolytic enzymes contribute to the progression of various cancers. We previously reported increased expression of the proline specific peptidases dipeptidyl peptidase-IV (DPP-IV) and its closest paralogue fibroblast activation protein (FAP) in human glioblastomas. Here we analyze the molecular heterogeneity of DPP-IV and FAP in glioblastomas. METHODS: ELISA, isoelectric focusing, 1D and 2D electrophoresis followed by WB or enzyme overlay assay were utilized to analyze DPP-IV and FAP isoforms...
September 2017: Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia
https://www.readbyqxmd.com/read/28412280/evaluation-of-sample-preparation-protocols-for-spider-venom-profiling-by-maldi-tof-ms
#14
Ondřej Bočánek, Ondrej Šedo, Stano Pekár, Zbyněk Zdráhal
Spider venoms are highly complex mixtures containing biologically active substances with potential for use in biotechnology or pharmacology. Fingerprinting of venoms by Matrix-Assisted Laser Desorption-Ionization - Time of Flight Mass Spectrometry (MALDI-TOF MS) is a thriving technology, enabling the rapid detection of peptide/protein components that can provide comparative information. In this study, we evaluated the effects of sample preparation procedures on MALDI-TOF mass spectral quality to establish a protocol providing the most reliable analytical outputs...
July 2017: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/28056387/mamp-microbe-associated-molecular-pattern-induced-changes-in-plasma-membrane-associated-proteins
#15
Hana Uhlíková, Martin Solanský, Vendula Hrdinová, Ondrej Šedo, Tomáš Kašparovský, Jan Hejátko, Jan Lochman
Plant plasma membrane associated proteins play significant roles in Microbe-Associated Molecular Pattern (MAMP) mediated defence responses including signal transduction, membrane transport or energetic metabolism. To elucidate the dynamics of proteins associated with plasma membrane in response to cryptogein, a well-known MAMP of defence reaction secreted by the oomycete Phytophthora cryptogea, 2D-Blue Native/SDS gel electrophoresis of plasma membrane fractions was employed. This approach revealed 21 up- or down-regulated protein spots of which 15 were successfully identified as proteins related to transport through plasma membrane, vesicle trafficking, and metabolic enzymes including cytosolic NADP-malic enzyme and glutamine synthetase...
March 2017: Journal of Plant Physiology
https://www.readbyqxmd.com/read/28013217/strain-and-sex-differences-in-the-vestibular-and-systemic-toxicity-of-3-3-iminodipropionitrile-in-mice
#16
Pere Boadas-Vaello, Lara Sedó-Cabezón, Enrique Verdú, Jordi Llorens
The nitrile 3,3'-iminodipropionitrile (IDPN) causes a loss of hair cells in the vestibular epithelium of the inner ear in several species of both mammals and nonmammals. It is of interest as a model compound in ototoxicity and vestibular regeneration research, but its effects on the mouse, including the potential relevance of strain and sex differences for susceptibility, have not yet been thoroughly characterized. In this study, we compared the vestibular toxicity of IDPN in dose-response studies (0, 8, 12, 16, and 24 mmol/kg IDPN p...
March 1, 2017: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/28009716/precursors-of-pancreatic-cancer
#17
REVIEW
Přemysl Frič, Jan Škrha, Aleksi Šedo, Petr Bušek, Martin Laclav, Bohuš Bunganič, Miroslav Zavoral
Pancreatic cancer (PC) behaves very differently in comparison with other malignancies. Its incidence has been increasing continuously; mortality has not decreased, the diagnosis is frequently late, radical surgery is performed only in 15-20% of patients, and chemotherapy is only palliative. PC occurs in three different forms. Sporadic PC accounts for 90% of all PCs. Its most frequent form is the pancreatic ductal adenocarcinoma. The remaining 10% constitute two minority groups: familial PC (7%) and PC as a manifestation of a genetic cancer syndrome (3%)...
March 2017: European Journal of Gastroenterology & Hepatology
https://www.readbyqxmd.com/read/27807664/intercellular-crosstalk-in-human-malignant-melanoma
#18
REVIEW
Barbora Dvořánková, Pavol Szabo, Ondřej Kodet, Hynek Strnad, Michal Kolář, Lukáš Lacina, Eliška Krejčí, Ondřej Naňka, Aleksi Šedo, Karel Smetana
Incidence of malignant melanoma is increasing globally. While the initial stages of tumors can be easily treated by a simple surgery, the therapy of advanced stages is rather limited. Melanoma cells spread rapidly through the body of a patient to form multiple metastases. Consequently, the survival rate is poor. Therefore, emphasis in melanoma research is given on early diagnosis and development of novel and more potent therapeutic options. The malignant melanoma is arising from melanocytes, cells protecting mitotically active keratinocytes against damage caused by UV light irradiation...
May 2017: Protoplasma
https://www.readbyqxmd.com/read/27802310/non-enzymatic-oligomerization-of-3-5-cyclic-amp
#19
Giovanna Costanzo, Samanta Pino, Anna Maria Timperio, Judit E Šponer, Jiří Šponer, Olga Nováková, Ondrej Šedo, Zbyněk Zdráhal, Ernesto Di Mauro
Recent studies illustrate that short oligonucleotide sequences can be easily produced from nucleotide precursors in a template-free non-enzymatic way under dehydrating conditions, i.e. using essentially dry materials. Here we report that 3',5' cyclic AMP may also serve as a substrate of the reaction, which proceeds under moderate conditions yet with a lower efficiency than the previously reported oligomerization of 3',5' cyclic GMP. Optimally the oligomerization requires (i) a temperature of 80°C, (ii) a neutral to alkaline environment and (iii) a time on the order of weeks...
2016: PloS One
https://www.readbyqxmd.com/read/27798859/ageing-as-an-important-risk-factor-for-cancer
#20
REVIEW
Karel Smetana, Lukáš Lacina, Pavol Szabo, Barbora Dvořánková, Prokop Brož, Aleksi Šedo
An ageing population is a typical feature of many developed countries across the world. Analyzed from a biomedical and philosophical point of view, this phenomenon is also a potential risk factor for social sustainability of communities. The association between ageing and cancer seems to be more than apparent. Therefore, the further increase of epidemic-like incidence of malignant tumors in a population can be expected in the near future. Elderly people usually suffer from age-dependent diseases, and such polymorbidity can seriously affect the treatment of malignant tumors...
2016: Anticancer Research
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