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https://www.readbyqxmd.com/read/28412280/evaluation-of-sample-preparation-protocols-for-spider-venom-profiling-by-maldi-tof-ms
#1
Ondřej Bočánek, Ondrej Šedo, Stano Pekár, Zbyněk Zdráhal
Spider venoms are highly complex mixtures containing biologically active substances with potential for use in biotechnology or pharmacology. Fingerprinting of venoms by Matrix-Assisted Laser Desorption-Ionization - Time of Flight Mass Spectrometry (MALDI-TOF MS) is a thriving technology, enabling the rapid detection of peptide/protein components that can provide comparative information. In this study, we evaluated the effects of sample preparation procedures on MALDI-TOF mass spectral quality to establish a protocol providing the most reliable analytical outputs...
April 12, 2017: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/28056387/mamp-microbe-associated-molecular-pattern-induced-changes-in-plasma-membrane-associated-proteins
#2
Hana Uhlíková, Martin Solanský, Vendula Hrdinová, Ondrej Šedo, Tomáš Kašparovský, Jan Hejátko, Jan Lochman
Plant plasma membrane associated proteins play significant roles in Microbe-Associated Molecular Pattern (MAMP) mediated defence responses including signal transduction, membrane transport or energetic metabolism. To elucidate the dynamics of proteins associated with plasma membrane in response to cryptogein, a well-known MAMP of defence reaction secreted by the oomycete Phytophthora cryptogea, 2D-Blue Native/SDS gel electrophoresis of plasma membrane fractions was employed. This approach revealed 21 up- or down-regulated protein spots of which 15 were successfully identified as proteins related to transport through plasma membrane, vesicle trafficking, and metabolic enzymes including cytosolic NADP-malic enzyme and glutamine synthetase...
December 14, 2016: Journal of Plant Physiology
https://www.readbyqxmd.com/read/28013217/strain-and-sex-differences-in-the-vestibular-and-systemic-toxicity-of-3-3-iminodipropionitrile-in-mice
#3
Pere Boadas-Vaello, Lara Sedó-Cabezón, Enrique Verdú, Jordi Llorens
The nitrile 3,3'-iminodipropionitrile (IDPN) causes a loss of hair cells in the vestibular epithelium of the inner ear in several species of both mammals and nonmammals. It is of interest as a model compound in ototoxicity and vestibular regeneration research, but its effects on the mouse, including the potential relevance of strain and sex differences for susceptibility, have not yet been thoroughly characterized. In this study, we compared the vestibular toxicity of IDPN in dose-response studies (0, 8, 12, 16, and 24 mmol/kg IDPN p...
March 1, 2017: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/28009716/precursors-of-pancreatic-cancer
#4
Přemysl Frič, Jan Škrha, Aleksi Šedo, Petr Bušek, Martin Laclav, Bohuš Bunganič, Miroslav Zavoral
Pancreatic cancer (PC) behaves very differently in comparison with other malignancies. Its incidence has been increasing continuously; mortality has not decreased, the diagnosis is frequently late, radical surgery is performed only in 15-20% of patients, and chemotherapy is only palliative. PC occurs in three different forms. Sporadic PC accounts for 90% of all PCs. Its most frequent form is the pancreatic ductal adenocarcinoma. The remaining 10% constitute two minority groups: familial PC (7%) and PC as a manifestation of a genetic cancer syndrome (3%)...
March 2017: European Journal of Gastroenterology & Hepatology
https://www.readbyqxmd.com/read/27807664/intercellular-crosstalk-in-human-malignant-melanoma
#5
REVIEW
Barbora Dvořánková, Pavol Szabo, Ondřej Kodet, Hynek Strnad, Michal Kolář, Lukáš Lacina, Eliška Krejčí, Ondřej Naňka, Aleksi Šedo, Karel Smetana
Incidence of malignant melanoma is increasing globally. While the initial stages of tumors can be easily treated by a simple surgery, the therapy of advanced stages is rather limited. Melanoma cells spread rapidly through the body of a patient to form multiple metastases. Consequently, the survival rate is poor. Therefore, emphasis in melanoma research is given on early diagnosis and development of novel and more potent therapeutic options. The malignant melanoma is arising from melanocytes, cells protecting mitotically active keratinocytes against damage caused by UV light irradiation...
May 2017: Protoplasma
https://www.readbyqxmd.com/read/27802310/non-enzymatic-oligomerization-of-3-5-cyclic-amp
#6
Giovanna Costanzo, Samanta Pino, Anna Maria Timperio, Judit E Šponer, Jiří Šponer, Olga Nováková, Ondrej Šedo, Zbyněk Zdráhal, Ernesto Di Mauro
Recent studies illustrate that short oligonucleotide sequences can be easily produced from nucleotide precursors in a template-free non-enzymatic way under dehydrating conditions, i.e. using essentially dry materials. Here we report that 3',5' cyclic AMP may also serve as a substrate of the reaction, which proceeds under moderate conditions yet with a lower efficiency than the previously reported oligomerization of 3',5' cyclic GMP. Optimally the oligomerization requires (i) a temperature of 80°C, (ii) a neutral to alkaline environment and (iii) a time on the order of weeks...
2016: PloS One
https://www.readbyqxmd.com/read/27798859/ageing-as-an-important-risk-factor-for-cancer
#7
REVIEW
Karel Smetana, Lukáš Lacina, Pavol Szabo, Barbora Dvořánková, Prokop Brož, Aleksi Šedo
An ageing population is a typical feature of many developed countries across the world. Analyzed from a biomedical and philosophical point of view, this phenomenon is also a potential risk factor for social sustainability of communities. The association between ageing and cancer seems to be more than apparent. Therefore, the further increase of epidemic-like incidence of malignant tumors in a population can be expected in the near future. Elderly people usually suffer from age-dependent diseases, and such polymorbidity can seriously affect the treatment of malignant tumors...
2016: Anticancer Research
https://www.readbyqxmd.com/read/27791322/temperature-controlled-switchable-photochromism-in-solid-materials
#8
Alex Julià-López, Jordi Hernando, Daniel Ruiz-Molina, Pablo González-Monje, Josep Sedó, Claudio Roscini
A novel strategy to achieve thermally switchable photochromism in solid materials is reported, which relies on the preparation of polymeric core-shell capsules containing solutions of photochromic dyes in acidic phase-change materials. Upon changing the phase (solid or liquid) of the encapsulated medium, one of the two photochromic states of the system is selectively stabilized on demand, allowing for reversible interconversion between direct and reverse photochromism when thermally scanning through the melting temperature of the phase-change material...
October 28, 2016: Angewandte Chemie
https://www.readbyqxmd.com/read/27769077/early-detection-of-pancreatic-cancer-impact-of-high-resolution-imaging-methods-and-biomarkers
#9
Přemysl Frič, Jan Škrha, Aleksi Šedo, Tomaš Zima, Petr Bušek, Klára Kmochová, Martin Laclav, Bohuš Bunganič, Svatopluk Solař, Petr Hrabal, František Bělina, Pavel Záruba, Pavel Škrha, Miroslav Zavoral
High-resolution imaging methods (HRIMs) and biomarkers present the second step of pancreatic cancer (PC) diagnostics in at-risk individuals. These include patients with positive risk factors, early symptoms, nonresponders to the initial antidiabetic therapy, patients older than 50 years of age with new-onset unstable diabetes requiring insulin as well as patients with long-term insulin-non-dependent diabetes and recent (up to 6 months) failure of antidiabetic therapy. The procedures should be started without delay and the co-operation between the primary and tertiary medical centers is highly desirable...
December 2016: European Journal of Gastroenterology & Hepatology
https://www.readbyqxmd.com/read/27689184/dynamic-change-of-focal-fatty-sparing-in-non-alcoholic-fatty-liver-disease-after-treatment-with-sitagliptin
#10
I Hoffmanová, P Škrha, A Šedo, M Anděl
No abstract text is available yet for this article.
September 2016: Ultrasound International Open
https://www.readbyqxmd.com/read/27644572/proximal-nail-fold-flap-for-digital-mucous-cyst-excision
#11
Yutaro Yamashita, Hiroaki Nagae, Ryosuke Yamato, Hiromichi Sedo, Yoshiro Abe, Ichiro Hashimoto
The skin covering a digital mucous cyst is often very thin and is often excised with the cyst. Thus, transfer of a skin flap is needed for the defect. We have developed a proximal nail fold flap technique by which the thin skin covering the cyst can be preserved. We conducted a retrospective study to assess the effectiveness and reliability of this technique for digital mucous cyst excision. The study group comprised 26 patients treated for 28 digital mucous cysts. The flap was elevated on the nail matrix to expose the distal interphalangeal joint capsule...
2016: Journal of Medical Investigation: JMI
https://www.readbyqxmd.com/read/27492457/fibroblast-activation-protein-alpha-is-expressed-by-transformed-and-stromal-cells-and-is-associated-with-mesenchymal-features-in-glioblastoma
#12
COMPARATIVE STUDY
Petr Busek, Eva Balaziova, Ivana Matrasova, Marek Hilser, Robert Tomas, Martin Syrucek, Zuzana Zemanova, Evzen Krepela, Jaromir Belacek, Aleksi Sedo
Glioblastomas are deadly neoplasms resistant to current treatment modalities. Fibroblast activation protein (FAP) is a protease which is not expressed in most of the normal adult tissues but is characteristically present in the stroma of extracranial malignancies. FAP is considered a potential therapeutic target and is associated with a worse patient outcome in some cancers. The FAP localization in the glioma microenvironment and its relation to patient survival are unknown. By analyzing 56 gliomas and 15 non-tumorous brain samples, we demonstrate increased FAP expression in a subgroup of high-grade gliomas, in particular on the protein level...
October 2016: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/27416508/cadaveric-and-radiologic-study-of-the-anatomical-variations-of-the-prostatic-arteries-a-review-of-the-literature-and-a-new-classification-proposal-with-application-to-prostatectomy
#13
REVIEW
Celia Moya, Julián Cuesta, Alfonsina Friera, Jose María Gil-Vernet Sedó, Francisco J Valderrama-Canales
Development of prostatic arterial embolization (PAE) to treat benign prostatic hyperplasia (BPH) has raised interest in the variations of the prostatic arteries (PA). The aim of this study is to identify these vascular variations, to compare them with previous data, and to propose a simple classification. Ten adult male pelvis sides from embalmed cadavers were dissected, ages 69 to 92 years, and 10 PA were examined. In a retrospective analysis of 34 DSA pelvic angiographies on 28 patients aged 50 to 90 years, 48 PA were identified...
January 2017: Clinical Anatomy
https://www.readbyqxmd.com/read/27394989/comparative-proteomic-analysis-of-sulfur-oxidizing-acidithiobacillus-ferrooxidans-ccm-4253-cultures-having-lost-the-ability-to-couple-anaerobic-elemental-sulfur-oxidation-with-ferric-iron-reduction
#14
COMPARATIVE STUDY
Jiri Kucera, Ondrej Sedo, David Potesil, Oldrich Janiczek, Zbynek Zdrahal, Martin Mandl
In extremely acidic environments, ferric iron can be a thermodynamically favorable electron acceptor during elemental sulfur oxidation by some Acidithiobacillus spp. under anoxic conditions. Quantitative 2D-PAGE proteomic analysis of a resting cell suspension of a sulfur-grown Acidithiobacillus ferrooxidans CCM 4253 subculture that had lost its iron-reducing activity revealed 147 protein spots that were downregulated relative to an iron-reducing resting cell suspension of the antecedent sulfur-oxidizing culture and 111 that were upregulated...
September 2016: Research in Microbiology
https://www.readbyqxmd.com/read/27388675/the-effect-of-dipeptidyl-peptidase-iv-inhibition-on-circulating-t-cell-subpopulations-in-patients-with-type-2-diabetes-mellitus
#15
Lucie Sromova, Petr Busek, Helena Posova, Jana Potockova, Pavel Skrha, Michal Andel, Aleksi Sedo
AIM: To assess intraindividually the effects of DPP-IV inhibition on the subpopulations of immune cells in type 2 diabetes mellitus (DM2) patients during the course of treatment with sitagliptin. METHODS: In this open label non-randomized observational study with a control group DM2 patients were examined before the initiation of the DPP-IV inhibitor administration (sitagliptin 100mg once daily) and then after 4weeks and 12months. Inhibition of the blood plasma DPP-IV enzymatic activity was determined by a chromogenic assay, the immunophenotyping of the blood cell subpopulations was performed using flow cytometry and blood plasma cytokine concentrations were quantified using an array-based multiplex ELISA...
August 2016: Diabetes Research and Clinical Practice
https://www.readbyqxmd.com/read/27357126/cleavage-stage-versus-blastocyst-stage-embryo-transfer-in-assisted-reproductive-technology
#16
REVIEW
Demián Glujovsky, Cindy Farquhar, Andrea Marta Quinteiro Retamar, Cristian Roberto Alvarez Sedo, Deborah Blake
BACKGROUND: Advances in cell culture media have led to a shift in in vitro fertilisation (IVF) practice from cleavage stage embryo transfer to blastocyst stage transfer. The rationale for blastocyst transfer is to improve both uterine and embryonic synchronicity and enable self selection of viable embryos, thus resulting in better live birth rates. OBJECTIVES: To determine whether blastocyst stage (day 5 to 6) embryo transfers improve the live birth rate, and other associated outcomes, compared with cleavage stage (day 2 to 3) embryo transfers...
June 30, 2016: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/27320722/increased-tissue-and-circulating-levels-of-dipeptidyl-peptidase-iv-enzymatic-activity-in-patients-with-pancreatic-ductal-adenocarcinoma
#17
Petr Busek, Zdislava Vanickova, Petr Hrabal, Marek Brabec, Premysl Fric, Miroslav Zavoral, Jan Skrha, Klara Kmochova, Martin Laclav, Bohus Bunganic, Koen Augustyns, Pieter Van Der Veken, Aleksi Sedo
BACKGROUND/OBJECTIVES: Pancreatic ductal adenocarcinoma (PDAC) is frequently heralded by an impairment of glucose homeostasis. Dipeptidyl peptidase-IV (DPP-IV) and fibroblast activation protein alpha (FAP) are aminopeptidases that regulate several bioactive peptides involved in glucoregulation, and are frequently dysregulated in cancer. The present study analyzes blood plasma levels and the quantity and localization of DPP-IV and FAP in PDAC tissues. METHODS: DPP-IV and FAP concentration and enzymatic activity were evaluated in the plasma from 93 PDAC, 39 type 2 diabetes mellitus (T2DM) and 29 control subjects, and in matched paired non-tumorous and tumor tissues from 48 PDAC patients...
September 2016: Pancreatology: Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]
https://www.readbyqxmd.com/read/27206091/relationship-between-sperm-dna-fragmentation-and-nuclear-vacuoles
#18
Mariano Lavolpe, Daniela Lorenzi, Ezequiel Greco, Florencia Nodar, Cristian Alvarez Sedó
OBJECTIVE: The aim of the present study is to assess the correlation between the presence, quantity and size of nuclear vacuoles and DNA damage and chromatin status in sperm samples of men who underwent to assisted reproduction technology. METHODS: Forty six males who underwent to assisted reproductive technology (ART) were considered. According to their latest semen analysis (<3 months), were grouped into: (A) strict morphology index ≤4% (26) and (B) strict morphology index ≥14% (20)...
2015: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/27206089/correlation-between-cytoplamic-oocyte-maturation-and-chromosomal-aneuploidies-impact-on-fertilization-embryo-quality-and-pregnancy
#19
Cristian Alvarez Sedó, Mariana Miguens, Sara Andreucci, Noelia Ortiz, Daniela Lorenzi, Sergio Papier, Florencia Nodar
OBJECTIVE: To establish the relationship between oocyte cytoplasmic maturation and its chromosomal status and determine the effect of this feature over the reproductive outcome in patients with sub-optimal fertilization in ART. METHODS: Fifty couples who underwent ART were selected. From nineteen patients, 22 metaphase II-MII and 18 failed-fertilized oocytes after ICSI were studied. The first polar body was collected for chromosomal analysis by aCGH. Oocytes were processed by immunocytochemistry (ICC) to determine oocyte maturation: assessment of inactive MPF status and the conformation-alignment of the metaphase plate...
2015: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/27120389/early-pancreatic-carcinogenesis-risk-factors-early-symptoms-and-the-impact-of-antidiabetic-drugs
#20
Přemysl Frič, Jan Škrha, Aleksi Šedo, Petr Bušek, Klára Kmochová, Martin Laclav, Svatopluk Solař, Bohuš Bunganič, Miroslav Zavoral
Risk factors (long-term diabetes, obesity) and early symptoms (new-onset diabetes, loss of weight, or persistent low body mass) are the initial symptoms of pancreatic carcinogenesis. They may be influenced by antidiabetic drugs and their correct evaluation is a prerequisite for early diagnosis of pancreatic cancer (PC). We review the risk factors, early symptoms, and the impact of antidiabetic drugs on early pancreatic carcinogenesis. The main source of data was the database Medline/PubMed and abstracts of international congresses (DDW, UEGW)...
July 2016: European Journal of Gastroenterology & Hepatology
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