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https://www.readbyqxmd.com/read/29299895/community-acquired-escherichia-coli-enteritis-in-korean-children-the-clinical-application-of-a-stool-polymerase-chain-reaction-assay
#1
Youie Kim, Hyo Jin Kim, Sooyeon Lim, Kil Seong Bae, Seung Beom Han, Dae Chul Jeong, Jin Han Kang, Gook Jae Shin, Gun Dong Lee, Yeon Joon Park
BACKGROUND: Although Escherichia coli is a common cause of bacterial enteritis in Korea, reports on community-acquired E. coli enteritis in Korean children are scarce. This study aimed to determine the clinical characteristics and pathotype distribution of community-acquired E. coli enteritis diagnosed by a multiplex polymerase chain reaction (PCR) assay in Korean children. MATERIALS AND METHODS: The medical records of children aged 18 years or less who were diagnosed with acute gastroenteritis by the attending physician between 2013 and 2016 were retrospectively reviewed...
December 2017: Infection & Chemotherapy
https://www.readbyqxmd.com/read/29290230/antimicrobial-treatment-of-urinary-tract-infections-in-children
#2
R Cohen, J Raymond, E Launay, Y Gillet, P Minodier, F Dubos, E Grimprel
Urinary tract infections are the most frequent documented bacterial infections in children. The antibiotic choices proposed in this manuscript are based on the guidelines published by the Pediatric Infectious Disease Group (GPIP) and the French-Language Infectious Disease Society (SPILF). Dipstick positive for leukocytes and/or nitrites must precede in most circumstances (excluding the newborns, neutropenic patients and those with sepsis), urine culture and antibiotic prescription. The proportion of extended-spectrum β-lactamase (ESBL) Escherichia coli strains has increased steadily in recent years, and the situations in which oral antibiotic switch is frequently not available are increasing...
December 2017: Archives de Pédiatrie: Organe Officiel de la Sociéte Française de Pédiatrie
https://www.readbyqxmd.com/read/29277142/carriage-of-multidrug-resistant-bacteria-among-pediatric-patients-before-and-during-their-hospitalization-in-a-tertiary-pediatric-unit-in-tunisia
#3
Miniar Tfifha, Asma Ferjani, Manel Mallouli, Nesrine Mlika, Saoussen Abroug, Jalel Boukadida
The pandemic spread of multidrug-resistant (MDR) bacteria (i.e., methicillin-resistant Staphylococcus aureus (MRSA), extended-spectrum b-lactamase-producing Enterobacteriaceae (ESBLPE), vancomycin-resistant enterococci, carbapenemase-producing Enterobacteriaceae (CPE), multiresistant Pseudomonas aeruginosa and multiresistant Acinetobacter baumannii) pose a threat to healthcare Worldwide. We found limited data of MDR bacteria in pediatric patients hospitalized in Tunisian tertiary healthcare.The aim of the study is to evaluate the acquisition rate of MDR acquisition during hospitalization and to explore some of the associated risk factors for both carriage and acquisition at the pediatric department, Sahloul University Hospital...
December 2018: Libyan Journal of Medicine
https://www.readbyqxmd.com/read/29262494/-epidemiological-and-etiological-surveillance-study-of-infectious-diarrhea-in-shanghai-in-2013-2015
#4
J Li, H Pan, W J Xiao, X H Gong, Y Zhuang, X Z Kuang, H Y Wu, Z A Yuan
Objective: To analyze the pathogen spectrum and epidemiological characters of infectious diarrhea cases in Shanghai from July 2013 to June 2015. Methods: From July 2013 to June 2015, using multi-stage sampling to select 22 hospitals as adult diarrhea surveillance sentinels, and 3 district central hospitals together with Shanghai municipal children's hospital as children diarrhea surveillance sentinels. A total of 6 267 diarrhea cases were sampled. The demographic characters, main clinical manifestations and signs of cases were investigated, and stool specimens were collected to conduct the bacteria culture and virus nucleic acid detection...
December 6, 2017: Zhonghua Yu Fang Yi Xue za Zhi [Chinese Journal of Preventive Medicine]
https://www.readbyqxmd.com/read/29259822/efficacy-of-a-bovine-colostrum-and-egg-based-intervention-in-acute-childhood-diarrhoea-in-guatemala-a-randomised-double-blind-placebo-controlled-trial
#5
James T Gaensbauer, Mario A Melgar, Diva M Calvimontes, Molly M Lamb, Edwin J Asturias, Ingrid L Contreras-Roldan, Samuel R Dominguez, Christine C Robinson, Stephen Berman
Background: Treatments for paediatric diarrhoeal disease are limited. We assessed the impact of a bovine colostrum and egg-based treatment designed to reduce diarrhoea duration through non-specific and pathogen-directed mechanisms in children. Methods: Randomised, double-blind, placebo-controlled trial of PTM202, derived from bovine colostrum and hyperimmune hen's egg on the duration of acute diarrhoeal disease in Guatemalan children. PTM202 contains specific immunoglobulins that target rotavirus, enterotoxigenic Escherichia coli, Shiga toxin-producing E...
2017: BMJ Global Health
https://www.readbyqxmd.com/read/29246958/antibody-profiling-of-kawasaki-disease-using-escherichia-coli-proteome-microarrays
#6
Ho-Chang Kuo, Ying-Hsien Huang, Feng-Hsiang Chung, Po-Chung Chen, Tzu-Cheng Sung, Yi-Wen Chen, Kai-Sheng Hsieh, Chien-Sheng Chen, Guan-Da Syu
Kawasaki disease (KD) is a form of systemic vasculitis that generally occurs in children under 5 years old. Currently, KD is still diagnosed according to its clinical symptoms, including prolonged fever, skin rash, conjunctivitis, neck lymphadenopathy, palm erythema, and oral mucosa changes. Since KD is a type of inflammation without specific marker for diagnosis, we plan to profile the plasma antibodies by using E. coli proteome microarray and analyze the differences between KD and healthy subjects. Plasmas were collected from KD patient before intravenous immunoglobulin treatment (KD1), at least 3 weeks after treatment (KD3), non-fever control (NC), and fever control (FC) children...
December 15, 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/29246196/a-two-month-follow-up-evaluation-testing-interventions-to-limit-the-emergence-and-spread-of-antimicrobial-resistant-bacteria-among-maasai-of-northern-tanzania
#7
Casey J Roulette, Mark A Caudell, Jennifer W Roulette, Robert J Quinlan, Marsha B Quinlan, Murugan Subbiah, Douglas R Call
BACKGROUND: In sub-Saharan Africa, efforts to control antimicrobial resistance (AMR) are aggravated by unregulated drug sales and use, and high connectivity between human, livestock, and wildlife populations. Our previous research indicates that Maasai agropastoralists-who have high exposure to livestock and livestock products and self-administer veterinary antibiotics-harbor antibiotic resistant Escherichia coli (E. coli). Here, we report the results of a public health intervention project among Maasai aimed at reducing selection and transmission of E...
December 15, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/29236304/enterovirus-detection-in-patients-with-acute-gastroenteritis-in-switzerland
#8
Rainer Gosert, Ulrich Heininger, Hans H Hirsch
Acute gastroenteritis (GE) has a major impact on morbidity and mortality worldwide, yet comprehensive data regarding infectious agents including enteroviruses are scarce. We hypothesized that enteroviruses constitute a significant cause of acute GE. We analyzed 677 stool samples from 504 patients, which had been submitted for suspected infectious GE. 0.2 ml of stool suspension was extracted using the Abbott m2000 sp robot and analysed by multiplex nucleic acid testing (NAT) using the Luminex xTAG gastrointestinal pathogen panel (GPP) as well as by specific NATs detecting enteroviruses and polioviruses...
December 13, 2017: Journal of Medical Virology
https://www.readbyqxmd.com/read/29233495/antibacterial-resistance-patterns-of-extended-spectrum-%C3%AE-lactamase-producing-enteropathogenic-escherichia-coli-strains-isolated-from-children
#9
Pejman Karami, Hassan Bazmamoun, Iraj Sedighi, Amir Sasan Mozaffari Nejad, Mohammad Mehdi Aslani, Mohammad Yousef Alikhani
BACKGROUND AND STUDY AIM: This study aimed to determine the antibacterial resistance patterns of extended spectrum β-lactamase (ESBL)-producing enteropathogenic Escherichia coli (EPEC) isolated from Iranian children and to investigate its genetic patterns. PATIENTS AND METHODS: 192 non-repeats EPEC isolates were collected from stool samples of the children with and without diarrhoea. The EPEC strains were isolated from 1355 stool specimens obtained from 247 children with diarrhoea (0-10 years old; mean age, 5...
December 7, 2017: Arab Journal of Gastroenterology: the Official Publication of the Pan-Arab Association of Gastroenterology
https://www.readbyqxmd.com/read/29226143/self-conjugation-of-the-enteropathogenic-escherichia-coli-adherence-factor-plasmid-of-four-typical-epec-isolates
#10
Claudia Silva, Crispín Zavala-Alvarado, José L Puente
The enteropathogenic Escherichia coli (EPEC) adherence factor plasmid (pEAF) encodes the proteins involved in the biogenesis of the bundle-forming pilus (BFP), a key virulence factor that mediates microcolony formation and the localized adherence phenotype on the surface of the host enterocytes. The presence or absence of this plasmid defines typical EPEC (tEPEC) and atypical EPEC (aEPEC), respectively. Although lateral transfer of pEAF has been evidenced by phylogenetic studies, conjugal transfer ability has been experimentally established only for two pEAF plasmids from strains isolated in the late 60s...
2017: BioMed Research International
https://www.readbyqxmd.com/read/29211769/arsenic-exposure-and-intestinal-microbiota-in-children-from-sirajdikhan-bangladesh
#11
Xiaoxi Dong, Natalia Shulzhenko, Julien Lemaitre, Renee L Greer, Kate Peremyslova, Quazi Quamruzzaman, Mahmudar Rahman, Omar Sharif Ibn Hasan, Sakila Afroz Joya, Mostofa Golam, David C Christiani, Andriy Morgun, Molly L Kile
BACKGROUND: Arsenic has antimicrobial properties at high doses yet few studies have examined its effect on gut microbiota. This warrants investigation since arsenic exposure increases the risk of many diseases in which gut microbiota have been shown to play a role. We examined the association between arsenic exposure from drinking water and the composition of intestinal microbiota in children exposed to low and high arsenic levels during prenatal development and early life. RESULTS: 16S rRNA gene sequencing revealed that children with high arsenic exposure had a higher abundance of Proteobacteria in their stool compared to matched controls with low arsenic exposure...
2017: PloS One
https://www.readbyqxmd.com/read/29207604/canis-familiaris-allergen-can-f-6-expression-purification-and-analysis-of-b-cell-epitopes-in-chinese-dog-allergic-children
#12
Yu-Jie Wang, Lin Li, Wei-Juan Song, Yan-Jun Zhou, Meng-Da Cao, Xiang-Rong Zuo, Ji-Fu Wei
Dog allergy is common worldwide. However, the allergenicity of dog allergy is still unclear in China as well as in special group, such as children. In this study, we chose Can f 6, a major dog allergen which belongs to the lipocalin to study its allergenicity in Chinese dog allergic children. Can f 6 gene was subcloned into pET-28a vector and transformed into E. coli BL21 (DE3) cells for expression. The recombinant Can f 6 was purified by nickel affinity chromatography, identified by SDS-PAGE, and tested for its allergenicity by Western blot with sera and basophil activation test...
October 31, 2017: Oncotarget
https://www.readbyqxmd.com/read/29189715/microbial-risk-assessment-of-tidal-induced-urban-flooding-in-can-tho-city-mekong-delta-vietnam
#13
Hong Quan Nguyen, Thi Thao Nguyen Huynh, Assela Pathirana, Peter Van der Steen
Public health risks from urban flooding are a global concern. Contaminated floodwater may expose residents living in cities as they are in direct contact with the water. However, the recent literature does not provide much information about this issue, especially for developing countries. In this paper, the health risk due to a flood event occurred in Can Tho City (Mekong Delta, Vietnam) on 7 October 2013 was investigated. The Quantitative Microbial Risk Assessment method was used in this study. The data showed that the pathogen concentrations were highly variable during the flood event and exceeded water standards for surface water...
November 30, 2017: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/29185342/comparative-proteomics-of-enterotoxigenic-escherichia-coli-reveals-differences-in-surface-protein-production-and-similarities-in-metabolism
#14
Veronika Kucharova Pettersen, Hans Steinsland, Harald G Wiker
Enterotoxigenic Escherichia coli (ETEC) infections are an important cause of diarrhoea among young children living in low- and middle-income countries and visiting travellers. The development of effective vaccines is complicated by substantial genomic diversity that exists among ETEC isolates. To investigate how ETEC genomic variation is reflected at expressed proteome level, we applied label-free quantitative proteomics to 7 human ETEC strains representing 5 epidemiologically important lineages. We further determined the proteome profile of the non-pathogenic E...
November 29, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/29165756/the-pathogenic-detection-for-126-children-with-diarrhea-and-drug-sensitivity-tests
#15
X-X Song, D-H Fang, Y-Q Quan, D-J Feng
OBJECTIVE: To investigate the cause of infective diarrhea in children, special distribution of the infection and the drug sensitivity. PATIENTS AND METHODS: We carried out pathogenic detection and drug sensitivity tests for one hundred and twenty-six children with diarrhea, including 67 children in infant group (0-<1 year) and 59 children in child group (1-5 years). RESULTS: Fat particles and starch particles were seen in most stool samples under microscope; 42 kinds of pathogenic bacteria were obtained from 126 stool samples, including 10 Diarrheagenic E...
October 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29151781/molecular-epidemiology-of-extended-spectrum-%C3%AE-lactamase-producing-escherichia-coli-pathotypes-in-diarrheal-children-from-low-socioeconomic-status-communities-in-bihar-india-emergence-of-the-ctx-m-type
#16
Abhishek Mandal, Abhishek Sengupta, Ajay Kumar, Utpal K Singh, Anil K Jaiswal, Pradeep Das, Sushmita Das
Background: Childhood diarrheal diseases remain highly endemic in India, but the emergence of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli among children with diarrhea in Bihar remains elusive. In this study, we determine and characterize ESBL-producing E coli pathotypes among hospitalized diarrheal preschool children living in low socioeconomic level communities in Bihar, India. Materials and methods: The stool samples were collected everyday throughout the year for 2 consecutive years...
2017: Infectious Diseases
https://www.readbyqxmd.com/read/29141754/effectiveness-of-the-hydrogen-sulfide-test-as-a-water-quality-indicator-for-diarrhea-risk-in-rural-bangladesh
#17
Mahfuza Islam, Ayse Ercumen, Abu Mohd Naser, Leanne Unicomb, Mahbubur Rahman, Benjamin F Arnold, John M Colford, Stephen P Luby
Microbiological water quality is usually assessed by the identification of Escherichia coli (E. coli), a fecal indicator. The hydrogen sulfide (H2S) test is an inexpensive, easy-to-use, and portable alternative field-based water quality test. Our study evaluated the H2S test's effectiveness as a water quality indicator for diarrhea risk. Field workers collected stored drinking water samples for H2S analysis and detection of E. coli by membrane filtration and measured caregiver-reported diarrhea among children < 5 years in the same households 1 month later...
October 16, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/29141752/differences-in-nutritional-and-health-status-in-school-children-from-the-highlands-and-lowlands-of-bolivia
#18
Graciela Terán, Washington Cuna, Froilán Brañez, Kristina E M Persson, Martín E Rottenberg, Susanne Nylén, Celeste Rodriguez
Children in the Bolivian Andes are exposed to endemic infections and meager nourishment, and live under poor hygienic conditions. The prevention of children malnutrition is a priority in many countries including Bolivia. In this study, the health status of schoolchildren in Taraco, a Puna district, at 4,000 meters above sea level (masl) and in Caranavi, at 650 masl in the wealthier subtropical valleys, was compared. The weight, height, and hematological and biochemical parameters in blood, parasites in stool, and clinical information in 120 children from rural Taraco and in 96 from semi-urban Caranavi, both predominantly of Aymara ethnicity, were registered...
November 6, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/29140236/impact-of-childhood-nutritional-status-on-pathogen-prevalence-and-severity-of-acute-diarrhea
#19
Kirkby D Tickell, Patricia B Pavlinac, Grace C John-Stewart, Donna M Denno, Barbra A Richardson, Jaqueline M Naulikha, Ronald K Kirera, Brett E Swierczewski, Benson O Singa, Judd L Walson
Children with acute and chronic malnutrition are at increased risk of morbidity and mortality following a diarrheal episode. To compare diarrheal disease severity and pathogen prevalence among children with and without acute and chronic malnutrition, we conducted a cross-sectional study of human immunodeficiency virus-uninfected Kenyan children aged 6-59 months, who presented with acute diarrhea. Children underwent clinical and anthropometric assessments and provided stool for bacterial and protozoal pathogen detection...
November 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/29138582/once-daily-intramuscular-amikacin-for-outpatient-treatment-of-lower-urinary-tract-infections-caused-by-extended-spectrum-%C3%AE-lactamase-producing-escherichia-coli-in-children
#20
Meltem Polat, Soner Sertan Kara
Background: The rise in community-acquired urinary tract infections (UTIs) with extended-spectrum β-lactamase (ESBL)-producing Escherichia coli strains raises the question of how to treat these infections effectively in pediatric outpatients. Amikacin has shown promising in vitro activity against ESBL-producing urinary isolates of E. coli; however, clinical data are limited. Objective: To investigate the clinical and microbiological outcomes of community-acquired lower UTIs caused by ESBL-producing E...
2017: Infection and Drug Resistance
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