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e. coli in children

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https://www.readbyqxmd.com/read/28793024/socioenvironmental-conditions-and-intestinal-parasitic-infections-in-brazilian-urban-slums-a-cross-sectional-study
#1
Caroline Ferraz Ignacio, Milena Enderson Chagas da Silva, Natasha Berendonk Handam, Maria de Fatima Leal Alencar, Adriana Sotero-Martins, Martha Macedo de Lima Barata, Antonio Henrique Almeida de Moraes
Methods: A cross-sectional study covering an agglomeration of urban slums was conducted between 2015 and 2016 using participants observation, a socioeconomic survey, and the spontaneous sedimentation method with three slides per sample to analyze fresh stool specimens ( n =595) searching for intestinal parasites. Results: Endolimax nana ( n =95, 16.0%) and Entamoeba coli ( n =65, 10.9%) were the most frequently identified agents, followed by Giardia intestinalis ( n =24, 4...
August 7, 2017: Revista do Instituto de Medicina Tropical de São Paulo
https://www.readbyqxmd.com/read/28783751/animal-related-factors-associated-with-moderate-to-severe-diarrhea-in-children-younger-than-five-years-in-western-kenya-a-matched-case-control-study
#2
Anne Conan, Ciara E O'Reilly, Eric Ogola, J Benjamin Ochieng, Anna J Blackstock, Richard Omore, Linus Ochieng, Fenny Moke, Michele B Parsons, Lihua Xiao, Dawn Roellig, Tamer H Farag, James P Nataro, Karen L Kotloff, Myron M Levine, Eric D Mintz, Robert F Breiman, Sarah Cleaveland, Darryn L Knobel
BACKGROUND: Diarrheal disease remains among the leading causes of global mortality in children younger than 5 years. Exposure to domestic animals may be a risk factor for diarrheal disease. The objectives of this study were to identify animal-related exposures associated with cases of moderate-to-severe diarrhea (MSD) in children in rural western Kenya, and to identify the major zoonotic enteric pathogens present in domestic animals residing in the homesteads of case and control children...
August 4, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28780245/prevalence-and-virulence-gene-profiling-of-enteroaggregative-escherichia-coli-in-malnourished-and-nourished-brazilian-children
#3
Alexandre Havt, Ila Fn Lima, Pedro Hqs Medeiros, Marco Af Clementino, Ana Ks Santos, Marília Smg Amaral, Herlice N Veras, Mara Mg Prata, Noélia L Lima, Alessandra Di Moura, Álvaro M Leite, Alberto M Soares, José Q Filho, Eric R Houpt, James P Nataro, Richard L Guerrant, Aldo Am Lima
The impact of enteroaggregative E. coli (EAEC) infection on childhood malnutrition and inflammation has been suggested, regardless of diarrhea. We investigated whether EAEC and its virulence-related genes (VRGs) are associated with malnutrition in a case-control study. Children aged 6-24 months from Brazil were enrolled as malnourished if weight-for-age Z-score (WAZ) ≤ -2 and nourished if WAZ > -1. Stools were cultured and examined for E. coli. DNA was extracted from fecal isolates and tested for EAEC by polymerase chain reaction (PCR)...
July 4, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28751566/prolonged-versus-standard-native-e-coli-asparaginase-therapy-in-childhood-acute-lymphoblastic-leukemia-and-non-hodgkin-lymphoma-final-results-of-the-eortc-clg-randomized-phase-iii-trial-58951
#4
Veerle Mondelaers, Stefan Suciu, Barbara De Moerloose, Alina Ferster, Françoise Mazingue, Geneviève Plat, Karima Yakouben, Anne Uyttebroeck, Patrick Lutz, Vitor Costa, Nicolas Sirvent, Emmanuel Plouvier, Martine Munzer, Maryline Poirée, Odile Minckes, Frédéric Millot, Dominique Plantaz, Philip Maes, Claire Hoyoux, Hélène Cave', Pierre Rohrlich, Yves Bertrand, Yves Benoit
Asparaginase is an essential component of combination chemotherapy for childhood acute lymphoblastic leukemia and non-Hodgkin's lymphoma. The value of asparaginase was further addressed in a group of non-very high risk patients by comparing a prolonged (long-asparaginase) versus standard (short-asparaginase) native E.coli asparaginase treatment through a randomized question as part of the phase III 58951 trial of the European Organisation for Research and Treatment of Cancer Children's Leukemia Group. The main endpoint was disease-free survival...
July 27, 2017: Haematologica
https://www.readbyqxmd.com/read/28742106/epidemiology-of-enteroaggregative-escherichia-coli-infections-and-associated-outcomes-in-the-mal-ed-birth-cohort
#5
MULTICENTER STUDY
Elizabeth T Rogawski, Richard L Guerrant, Alexandre Havt, Ila F N Lima, Pedro H Q S Medeiros, Jessica C Seidman, Benjamin J J McCormick, Sudhir Babji, Dinesh Hariraju, Ladaporn Bodhidatta, Jasmin Shrestha, Japhat Anania, Athanasia Maro, Amidou Samie, Pablo Peñataro Yori, Shahida Qureshi, Mustafa Mahfuz, Pascal O Bessong, Margaret N Kosek, Tahmeed Ahmed, Zulfiqar A Bhutta, Dennis R Lang, Michael Gottlieb, Eric R Houpt, Aldo A M Lima
BACKGROUND: Enteroaggregative E. coli (EAEC) have been associated with mildly inflammatory diarrhea in outbreaks and in travelers and have been increasingly recognized as enteric pathogens in young children with and without overt diarrhea. We examined the risk factors for EAEC infections and their associations with environmental enteropathy biomarkers and growth outcomes over the first two years of life in eight low-resource settings of the MAL-ED study. METHODS: EAEC infections were detected by PCR gene probes for aatA and aaiC virulence traits in 27,094 non-diarrheal surveillance stools and 7,692 diarrheal stools from 2,092 children in the MAL-ED birth cohort...
July 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28742000/high-prevalence-of-multidrug-resistant-escherichia-coli-isolates-from-children-with-and-without-diarrhoea-and-their-susceptibility-to-the-antibacterial-activity-of-extracts-fractions-of-fruits-native-to-mexico
#6
Magdalena de Jesús Uribe-Beltrán, Yesmi Patricia Ahumada-Santos, Sylvia Páz Díaz-Camacho, Carlos Alberto Eslava-Campos, Jesús Ernesto Reyes-Valenzuela, María Elena Báez-Flores, Ignacio Osuna-Ramírez, Francisco Delgado-Vargas
PURPOSE: This paper aims to evaluate the antimicrobial resistance of Esherichia coli isolates from children under 5 years old, with and without diarrhoea, who were hospital outpatients in Culiacan, Sinaloa, Mexico. It also looks at the antimicrobial activity of fruit extracts against selected multidrug-resistant (MDR) E. coli strains. METHODOLOGY: A total of 205 E. coli isolates from stool samples were collected from 94 children under 5 years old who were outpatients from two hospitals in the city of Culiacan, Sinaloa, Mexico, during the autumn/winter of 2003/04; their resistance profiles to 19 commercial antimicrobials were investigated using the Kirby-Bauer method...
July 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28730135/clinical-characteristics-and-ultrasonographic-findings-of-acute-bacterial-enterocolitis-in-children
#7
Peter Chun, Taek Jin Lim, Eun Ha Hwang, Sang Wook Mun, Yeoun Joo Lee, Jae Hong Park
PURPOSE: This study clarified the bacterial pathogens currently causing acute infectious enterocolitis (AIE) in children and evaluated the clinical characteristics and ultrasonographic findings according to the different pathogens. METHODS: Medical records regarding age, sex, clinical symptoms, laboratory data, identified enteropathogens, ultrasonographic findings, treatment, and outcome of 34 patients who were diagnosed with AIE via stool examination using multiplex polymerase chain reaction (PCR) or culture, were retrospectively reviewed...
June 2017: Pediatric Gastroenterology, Hepatology & Nutrition
https://www.readbyqxmd.com/read/28729206/antimicrobial-resistance-patterns-of-gram-negative-bacteria-isolated-from-bloodstream-infections-in-an-iranian-referral-pediatric-hospital-a-six-year-study
#8
Shima Mahmoudi, Masoumeh Mahzari, Maryam Banar, Babak Pourakbari, Mohammad Taghi Haghi Ashtiani, Mohsen Mohammadi, Sepideh Keshavarz Valian, Setareh Mamishi
INTRODUCTION: Bloodstream infections (BSIs) are considered as a major cause of morbidity and mortality in children all over the world. The aim of this study was to describe the epidemiology and antimicrobial resistance patterns of Gram-negative bacteria (GNB) isolated from bloodstream infections (BSIs) in children admitted to the Children Medical Center Hospital (CMC), Tehran, Iran. METHODS: Clinical and microbiological data of the patients with positive blood cultures was collected from March 2011 to September 2016...
July 17, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28723927/piped-water-supply-interruptions-and-acute-diarrhea-among-under-five-children-in-addis-ababa-slums-ethiopia-a-matched-case-control-study
#9
Metadel Adane, Bezatu Mengistie, Girmay Medhin, Helmut Kloos, Worku Mulat
BACKGROUND: The problem of intermittent piped water supplies that exists in low- and middle-income countries is particularly severe in the slums of sub-Saharan Africa. However, little is known about whether there is deterioration of the microbiological quality of the intermittent piped water supply at a household level and whether it is a factor in reducing or increasing the occurrence of acute diarrhea among under-five children in slums of Addis Ababa. This study aimed to determine the association of intermittent piped water supplies and point-of-use (POU) contamination of household stored water by Escherichia coli (E...
2017: PloS One
https://www.readbyqxmd.com/read/28713083/regional-features-of-the-microbial-landscape-of-urine-and-comparative-evaluation-of-antimicrobial-therapy-in-children-with-cystitis
#10
Tetyana O Kryuchko, Vira P Kharshman, Inna M Nesina, Nataliia V Kuzmenko, Jaroslava M Oleksiienko
INTRODUCTION: Growing resistance of pathogens to antibiotics, including cross-resistance to other antimicrobial classes that are used in the treatment of recurrent infections of the lower urinary system in children demands constant control of issues of regional antibiotic resistance. In the present days, in the empirical treatment of such patients physicians still choose medications with preserved activity in relation to E. coli. The aim of our study was to investigate the regional features of microbial landscape of urine in children with cystitis and study the efficacy of 7-day administration of Furamag medicinal drug for the treatment of recurrent episodes of this disease in children...
2017: Wiadomości Lekarskie: Organ Polskiego Towarzystwa Lekarskiego
https://www.readbyqxmd.com/read/28712156/-unfit-for-human-consumption-a-study-of-the-contamination-of-formula-milk-fed-to-young-children-in-east-java-indonesia
#11
Sarah Gibson, Daniel Sahanggamu, Dewi Fatmaningrum, Val Curtis, Sian White
OBJECTIVES: To examine levels of bacterial contamination in formula feeding bottles in Sidoarjo, East Java, and to assess the preparation practices that may have been responsible. METHODS: A cross-sectional study was conducted among 92 randomly selected households with children under the age of two who were bottle-fed formula. In each household, we carried out video observation of mothers/caregivers preparing bottles, and examined samples of formula for coliform bacteria and Escherichia coli (E...
July 16, 2017: Tropical Medicine & International Health: TM & IH
https://www.readbyqxmd.com/read/28706384/antimicrobial-susceptibility-risk-factors-and-prevalence-of-bla-cefotaximase-temoneira-and-sulfhydryl-variable-genes-among-escherichia-coli-in-community-acquired-pediatric-urinary-tract-infection
#12
Kallyadan V Nisha, Shetty A Veena, Shenoy D Rathika, Shenoy M Vijaya, Shetty K Avinash
INTRODUCTION: The emergence of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli has become an important challenge among pediatric patients with community-acquired urinary tract infection (UTI). OBJECTIVES: The aim of this study was to assess the antimicrobial susceptibility patterns, associated risk factors and to survey the frequency of bla cefotaximase (CTX-M), bla temoneira (TEM), and bla sulfhydryl variable (SHV) genotypes in ESBL-producing E...
July 2017: Journal of Laboratory Physicians
https://www.readbyqxmd.com/read/28705515/combination-vaccine-strategies-to-prevent-enteric-infections
#13
Richard Walker, Peter Dull
New vaccine candidates entering the current routine immunization schedule can best be accommodated as combination vaccines. A combined Shigella and enterotoxigenic E. coli (ETEC) vaccine could greatly benefit children in disease-endemic areas. New candidates are getting closer to being able to meet these needs, but they raise numerous strategic questions related to presentation, formulation, and regulatory approach. The "Combination Vaccine Strategies to Prevent Enteric Infections" workshop at the 2016 Vaccines Against Shigella and ETEC (VASE) Conference examined some of the considerations for developing such vaccines against enteric pathogens...
July 10, 2017: Vaccine
https://www.readbyqxmd.com/read/28697828/-a-molecular-epidemiological-study-of-ki-polyomavirus-and-wu-polyomavirus-in-children-with-acute-respiratory-infection-in-tianjin-china
#14
Shu-Xiang Lin, Wei Wang, Wei Guo, Hong-Jiang Yang, Bai-Cheng Ma, Yu-Lian Fang, Yong-Sheng Xu
OBJECTIVE: To investigate the relationship of KI polyomavirus (KIPyV) and WU polyomavirus (WUPyV) with acute respiratory infection in children in Tianjin, China. METHODS: A total of 3 730 nasopharyngeal secretions were collected from hospitalized children with acute respiratory infection in Tianjin Children's Hospital from January 2011 to December 2013. Viral nucleic acid was extracted, and virus infection (KIPyV and WUPyV) was determined by PCR. Some KIPyV-positive and WUPyV-positive PCR products were subjected to sequencing...
July 2017: Zhongguo Dang Dai Er Ke za Zhi, Chinese Journal of Contemporary Pediatrics
https://www.readbyqxmd.com/read/28693504/prevalence-of-multi-drug-resistant-enteropathogenic-and-enteroinvasive-escherichia-coli-isolated-from-children-with-and-without-diarrhea-in-northeast-indian-population
#15
Karuppasamy Chellapandi, Tapan Kumar Dutta, Indu Sharma, Surajit De Mandal, Nachimuthu Senthil Kumar, Lalsanglura Ralte
BACKGROUND: Diarrheagenic Escherichia coli are associated with infantile diarrhea in the developing countries. The present study was conducted to determine the occurrence and antimicrobial resistance pattern of enteropathogenic and enteroinvasive E. coli associated with diarrhoea among the paediatric patients. METHODS: A total of 262 stool samples were collected from children with and without diarrhea from Mizoram, Northeast India. E. coli were isolated and subjected to multiplex PCR to detect virulent genes of EPEC (eaeA and bfpA) and EIEC (ial)...
July 11, 2017: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/28683780/bacterial-agents-and-antibiotic-resistance-profiles-of-infections-from-different-sites-that-occurred-among-patients-at-debre-markos-referral-hospital-ethiopia-a-cross-sectional-study
#16
Wondemagegn Mulu, Bayeh Abera, Mulat Yimer, Tadesse Hailu, Haimanot Ayele, Dereje Abate
BACKGROUND: In developing countries like Ethiopia, infections with antibiotic resistant bacteria become a real threat. Hence, monitoring of local level antimicrobial resistance profile is indispensable to contain the spread of drug resistant bacteria and intervene poor awareness on antimicrobial resistance. Therefore, this study aimed at determining bacterial and antibiotic resistance profiles of infections from different sites that occurred among patients. METHODS: Retrospective data recorded were analyzed on culture and drug susceptibility test results at Debre Markos Referral Hospital which were performed from 2011 to 2014...
July 6, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/28681807/spectrum-of-diarrhoeagenic-escherichia-coli-in-paediatric-population-suffering-from-diarrhoea-and-as-commensals-in-healthy-children
#17
Taru Singh, Shukla Das, V G Ramachandran, Sajad Ahmad Dar, K Snehaa, Rumpa Saha, Dheeraj Shah
BACKGROUND: Diarrhoeagenic Escherichia coli (DEC) is associated with early death of children in developing countries and are being identified now as an important evolving pathogen. The objective of this study was to perform multiplex polymerase chain reaction (PCR) for simultaneous detection of six categories of DEC in two sets of PCR reactions using 11 virulent genes. MATERIALS AND METHODS: During 1-year study period, forty isolates each from outpatient, inpatient and healthy groups were collected from children...
April 2017: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28674119/cancer-screening-recommendations-and-clinical-management-of-inherited-gastrointestinal-cancer-syndromes-in-childhood
#18
REVIEW
Maria Isabel Achatz, Christopher C Porter, Laurence Brugières, Harriet Druker, Thierry Frebourg, William D Foulkes, Christian P Kratz, Roland P Kuiper, Jordan R Hansford, Hector Salvador Hernandez, Katherine L Nathanson, Wendy K Kohlmann, Leslie Doros, Kenan Onel, Kami Wolfe Schneider, Sarah R Scollon, Uri Tabori, Gail E Tomlinson, D Gareth R Evans, Sharon E Plon
Hereditary gastrointestinal cancer predisposition syndromes have been well characterized, but management strategies and surveillance remain a major challenge, especially in childhood. In October 2016, the American Association for Cancer Research organized the AACR Childhood Cancer Predisposition Workshop in which international experts in care of children with a hereditary risk of cancer met to define surveillance strategies and management of children with cancer predisposition syndromes. In this article, we review the current literature in polyposis syndromes that can be diagnosed in childhood and may be associated with an increased incidence of gastrointestinal neoplasms and other cancer types...
July 1, 2017: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/28668691/colistin-resistance-gene-mcr-1-in-children-s-gut-flora
#19
Yan-Yan Hu, Yue-Ling Wang, Qiao-Ling Sun, Zi-Xian Huang, Han-Yu Wang, Rong Zhang, Gong-Xiang Chen
The aim of this study was to clarify the prevalence and transmission mechanism(s) of mcr-1 in children's gut flora. Fecal samples (n=173) were obtained from non-diarrhea patients at the Children's Hospital of Zhejiang University. Polymerase chain reaction-based analysis indicated that 17 isolates from 9.8% of the samples were positive for mcr-1; these comprised 16 E. coli isolates and one C. freundii. Nine mcr-1-bearing isolates co-expressed extended-spectrum beta-lactamase genes but plasmid-mediated quinolone resistance genes were not detected...
June 28, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28662145/prevalence-and-antibiotic-resistance-profiles-of-cerebrospinal-fluid-pathogens-in-children-with-acute-bacterial-meningitis-in-yunnan-province-china-2012-2015
#20
Hongchao Jiang, Min Su, Liyue Kui, Hailin Huang, Lijuan Qiu, Li Li, Jing Ma, Tingyi Du, Mao Fan, Qiangming Sun, Xiaomei Liu
Acute bacterial meningitis is still considered one of the most dangerous infectious diseases in children. To investigate the prevalence and antibiotic resistance profiles of cerebrospinal fluid (CSF) pathogens in children with acute bacterial meningitis in Southwest China, CSF samples from 179 meningitis patients (3 days to 12 years old) with positive culture results were collected from 2012 to 2015. Isolated pathogens were identified using the Vitek-32 system. Gram stain results were used to guide subcultures and susceptibility testing...
2017: PloS One
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