keyword
MENU ▼
Read by QxMD icon Read
search

Small molecular

keyword
https://www.readbyqxmd.com/read/29040814/inhibiting-polo-like-kinase-1-plk1-enhances-radiosensitization-via-modulating-dna-repair-proteins-in-non-small-cell-lung-cancer
#1
Da Yao, Peigui Gu, Youyu Wang, Weibin Luo, Huiliang Chi, Jianjun Ge, Youhui Qian
To assure the faithful chromosome segregation, cells make use of the spindle assembly checkpoint (SAC), which can be activated in aneuploidy cancer cells. In this study, the efficacies of inhibiting Polo-like kinase 1 (PLK1) on the radiosensitization of non-small cell lung cancer (NSCLC) cells were studied. Clonogenic survival assay was performed to identify the effects of the PLK1 inhibitor on radiosensitivity within NSCLC cells. Mitotic catastrophe assessment was used to measure the cell death and γH2AX foci were utilized to assess the DNA double-strand breaks (DSB)...
October 17, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/29040662/molecular-subtyping-of-tumors-from-patients-with-familial-glioma
#2
Vanessa Y Ruiz, Corinne E Praska, Georgina Armstrong, Thomas M Kollmeyer, Seiji Yamada, Paul A Decker, Matthew L Kosel, Jeanette E Eckel-Passow, The Gliogene Consortium, Daniel H Lachance, Matthew N Bainbridge, Beatrice S Melin, Melissa L Bondy, Robert B Jenkins
BACKGROUND: Single-gene mutation syndromes account for some familial glioma (FG); however, they make up only a small fraction of glioma families. Gliomas can be classified into 3 major molecular subtypes based on IDH mutation and 1p/19q co-deletion. We hypothesized that the prevalence of molecular subtypes might differ in familial versus sporadic gliomas, and that tumors in the same family should have the same molecular subtype. METHODS: Participants in the FG study (Gliogene) provided samples for germline DNA analysis...
October 10, 2017: Neuro-oncology
https://www.readbyqxmd.com/read/29040625/rise-a-database-of-rna-interactome-from-sequencing-experiments
#3
Jing Gong, Di Shao, Kui Xu, Zhipeng Lu, Zhi John Lu, Yucheng T Yang, Qiangfeng Cliff Zhang
We present RISE (http://rise.zhanglab.net), a database of RNA Interactome from Sequencing Experiments. RNA-RNA interactions (RRIs) are essential for RNA regulation and function. RISE provides a comprehensive collection of RRIs that mainly come from recent transcriptome-wide sequencing-based experiments like PARIS, SPLASH, LIGR-seq, and MARIO, as well as targeted studies like RIA-seq, RAP-RNA and CLASH. It also includes interactions aggregated from other primary databases and publications. The RISE database currently contains 328,811 RNA-RNA interactions mainly in human, mouse and yeast...
October 4, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/29040564/n-cadherin-identifies-human-endometrial-epithelial-progenitor-cells-by-in-vitro-stem-cell-assays
#4
Hong P T Nguyen, L Xiao, James A Deane, Ker-Sin Tan, Fiona L Cousins, Hirotaka Masuda, Carl N Sprung, Anna Rosamilia, Caroline E Gargett
STUDY QUESTION: Is there a specific surface marker that identifies human endometrial epithelial progenitor cells with adult stem cell activity using in vitro assays? SUMMARY ANSWER: N-cadherin isolates clonogenic, self-renewing human endometrial epithelial progenitor cells with high proliferative potential that differentiate into cytokeratin+ gland-like structures in vitro and identifies their location in some cells of gland profiles predominantly in basalis endometrium adjacent to the myometrium...
September 27, 2017: Human Reproduction
https://www.readbyqxmd.com/read/29040265/analysis-of-peripheral-b-cells-and-autoantibodies-against-the-anti-nicotinic-acetylcholine-receptor-derived-from-patients-with-myasthenia-gravis-using-single-cell-manipulation-tools
#5
Tomohiro Makino, Ryuichi Nakamura, Maki Terakawa, Satoshi Muneoka, Kazuhiro Nagahira, Yuriko Nagane, Jyoji Yamate, Masakatsu Motomura, Kimiaki Utsugisawa
The majority of patients with myasthenia gravis (MG), an organ-specific autoimmune disease, harbor autoantibodies that attack the nicotinic acetylcholine receptor (nAChR-Abs) at the neuromuscular junction of skeletal muscles, resulting in muscle weakness. Single cell manipulation technologies coupled with genetic engineering are very powerful tools to examine T cell and B cell repertoires and the dynamics of adaptive immunity. These tools have been utilized to develop mAbs in parallel with hybridomas, phage display technologies and B-cell immortalization...
2017: PloS One
https://www.readbyqxmd.com/read/29040080/detection-of-giardia-intestinalis-in-water-samples-collected-from-natural-water-reservoirs-and-wells-in-northern-and-north-eastern-poland-using-lamp-real-time-pcr-and-nested-pcr
#6
Anna Lass, Beata Szostakowska, Krzysztof Korzeniewski, Panagiotis Karanis
Giardia intestinalis is a protozoan parasite, transmitted to humans and animals by the faecal-oral route, mainly through contaminated water and food. Knowledge about the distribution of this parasite in surface water in Poland is fragmentary and incomplete. Accordingly, 36 environmental water samples taken from surface water reservoirs and wells were collected in Pomerania and Warmia-Masuria provinces, Poland. The 50 L samples were filtered and subsequently analysed with three molecular detection methods: loop-mediated isothermal amplification (LAMP), real-time polymerase chain reaction (real-time PCR) and nested PCR...
October 2017: Journal of Water and Health
https://www.readbyqxmd.com/read/29040058/recommendations-for-laboratory-containment-and-management-of-gene-drive-systems-in-arthropods
#7
Mark Q Benedict, Austin Burt, Margareth L Capurro, Paul De Barro, Alfred M Handler, Keith R Hayes, John M Marshall, Walter J Tabachnick, Zach N Adelman
Versatile molecular tools for creating driving transgenes and other invasive genetic factors present regulatory, ethical, and environmental challenges that should be addressed to ensure their safe use. In this article, we discuss driving transgenes and invasive genetic factors that can potentially spread after their introduction into a small proportion of individuals in a population. The potential of invasive genetic factors to increase their number in natural populations presents challenges that require additional safety measures not provided by previous recommendations regarding accidental release of arthropods...
October 17, 2017: Vector Borne and Zoonotic Diseases
https://www.readbyqxmd.com/read/29040003/dietary-risk-evaluation-for-28-polycyclic-aromatic-hydrocarbons-pahs-in-tea-preparations-made-of-teas-available-on-the-polish-retail-market
#8
Marek Roszko, Marta Kamińska, Krystyna Szymczyk, Renata Jędrzejczak
The aim of this work was to assess dietary risk resulting from consumption of polycyclic aromatic hydrocarbons (PAHs) with tea infusions. To this end, levels of 28 PAHs in black, green, red and white teas available on the Polish retail market have been assessed. Profiles and correlation between concentrations of individual PAHs have been identified. A model study on transfer of PAHs from tea leaves into tea preparations has been conducted. Relatively high concentrations of 28 evaluated PAHs have been found in 58 tested samples of black, green, red and white teas sampled on the Polish retail market...
October 17, 2017: Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes
https://www.readbyqxmd.com/read/29039951/1-2-fluorine-radical-rearrangements-isomerization-events-in-perfluorinated-radicals
#9
Daniel J Van Hoomissen, Shubham Vyas
Devising effective degradation technologies for perfluoroalkyl substances (PFASs) is an active area of research, where the molecular mechanisms involving both oxidative and reductive pathways are still elusive. One commonly neglected pathway in PFASs degradation is fluorine atom migration in perfluoroalkyl radicals, which was largely assumed to be implausible because of the high C-F bond strength. Using density functional theory calculations, it was demonstrated that 1,2-F atom migrations are thermodynamically favored when the fluorine atom migrated from less branched carbon center to a more branched carbon center...
October 17, 2017: Journal of Physical Chemistry. A
https://www.readbyqxmd.com/read/29039935/numerical-optimization-of-density-functional-tight-binding-models-application-to-molecules-containing-carbon-hydrogen-nitrogen-and-oxygen
#10
Aditi Krishnapriyan, Ping Yang, Anders M N Niklasson, Marc J Cawkwell
New parameterizations for semi-empirical density functional tight binding (DFTB) theory have been developed by the numerical optimization of adjustable parameters to minimize errors in the atomization energy and interatomic forces with respect to ab initio calculated data. Initial guesses for the radial dependences of the Slater-Koster bond integrals and overlap integrals were obtained from minimum basis density functional theory calculations. The radial dependences of the pair potentials and the bond and overlap integrals were represented by simple analytic functions...
October 17, 2017: Journal of Chemical Theory and Computation
https://www.readbyqxmd.com/read/29039925/exosomes-secreted-by-hela-cells-shuttle-on-their-surface-the-plasma-membrane-associated-sialidase-neu3
#11
Lucia Paolini, Flavia Orizio, Sara Busatto, Annalisa Radeghieri, Roberto Bresciani, Paolo Bergese, Eugenio Monti
Sialidases are glycohydrolases that remove terminal sialic acid residues from oligosaccharides, glycolipids and glycoproteins. The plasma membrane-associated sialidase NEU3 is involved in the fine tuning of sialic acid containing glycans directly on the cell surface and plays relevant roles in important biological phenomena such as cell differentiation, molecular recognition and cancer transformation. Extracellular vesicles are membranous structures with a diameter of 0.03-1 m released by cells and detectable in blood, urine, as well as in culture media...
October 17, 2017: Biochemistry
https://www.readbyqxmd.com/read/29036929/interaction-between-rho-gtpases-and-14-3-3-proteins
#12
REVIEW
Daniel Brandwein, Zhixiang Wang
The Rho GTPase family accounts for as many as 20 members. Among them, the archetypes RhoA, Rac1, and Cdc42 have been the most well-characterized. Like all members of the small GTPases superfamily, Rho proteins act as molecular switches to control cellular processes by cycling between active, GTP-bound and inactive, GDP-bound states. The 14-3-3 family proteins comprise seven isoforms. They exist as dimers (homo- or hetero-dimer) in cells. They function by binding to Ser/Thr phosphorylated intracellular proteins, which alters the conformation, activity, and subcellular localization of their binding partners...
October 15, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29036827/validation-by-magnetic-resonance-imaging-of-the-diagnostic-potential-of-a-heptapeptide-functionalized-imaging-probe-targeted-to-amyloid-%C3%AE-and-able-to-cross-the-blood-brain-barrier
#13
Séverine André, Emilie Ansciaux, Elamine Saidi, Lionel Larbanoix, Dimitri Stanicki, Denis Nonclercq, Luce Vander Elst, Sophie Laurent, Robert N Muller, Carmen Burtea
The diagnosis of Alzheimer's disease (AD) is a critical step in the management of patients. We have developed a non-invasive diagnosis tool based on magnetic resonance molecular imaging (MRMI) of amyloid-β peptide using ultra-small particles of iron oxide (USPIO) functionalized with a disulfide constrained cyclic heptapeptide (PHO) identified by phage display (USPIO-PHO). After previously demonstrating the optimal pharmacologic properties of USPIO-PHO and its capacity to cross the blood-brain barrier (BBB), the ability of USPIO-PHO to target amyloid plaques (AP) by MRMI has been validated in the present work on AD transgenic mice...
October 12, 2017: Journal of Alzheimer's Disease: JAD
https://www.readbyqxmd.com/read/29036204/metabolic-phenotyping-in-the-mouse-model-of-urinary-tract-infection-shows-that-3-hydroxybutyrate-in-plasma-is-associated-with-infection
#14
Pei Han, Yong Huang, Yumin Xie, Wu Yang, Yaoyao Wang, Wenying Xiang, Peter J Hylands, Cristina Legido-Quigley
Urinary tract infection is one of the most common bacterial infections worldwide. Current diagnosis of urinary tract infection chiefly relies on its clinical presentation, urine dipstick tests and urine culture. Small molecules found in bio-fluids related with both infection and recovery would facilitate diagnosis and management of UTI. Mass spectrometry-based fingerprinting of plasma and urine at 3 time points, pre-infection (t = -24h), infection (t = 24h) and post 3-day treatment (t = 112h), were acquired in the following four groups: mice which were healthy, infected but not treated, infected and treated with ciprofloxacin, and infected and treated with Relinqing® granules (n = 6 per group)...
2017: PloS One
https://www.readbyqxmd.com/read/29036200/the-effect-of-the-pathological-v72i-d109n-and-t190m-missense-mutations-on-the-molecular-structure-of-%C3%AE-dystroglycan
#15
Sonia Covaceuszach, Manuela Bozzi, Maria Giulia Bigotti, Francesca Sciandra, Petr V Konarev, Andrea Brancaccio, Alberto Cassetta
Dystroglycan (DG) is a highly glycosylated protein complex that links the cytoskeleton with the extracellular matrix, mediating fundamental physiological functions such as mechanical stability of tissues, matrix organization and cell polarity. A crucial role in the glycosylation of the DG α subunit is played by its own N-terminal region that is required by the glycosyltransferase LARGE. Alteration in this O-glycosylation deeply impairs the high affinity binding to other extracellular matrix proteins such as laminins...
2017: PloS One
https://www.readbyqxmd.com/read/29035861/the-molecular-identification-of-calodium-hepaticum-in-the-wild-brown-rat-rattus-norvegicus-in-poland
#16
Katarzyna Buńkowska-Gawlik, Agnieszka Perec-Matysiak, Klaudia Burzyńska, Joanna Hildebrand
Calodium hepaticum is a parasitic nematode found primarily in rodents but known to infect numerous other mammal species, and causing in humans the hepatic calodiasis. Herein, we present the first finding of C. hepaticum in Rattus norvegicus in Poland. In this study, we have used the combined pepsin-HCl digestion and molecular techniques to detect C. hepaticum infection in the liver. The results confirm the usefulness of molecular approaches for investigating the biology and epidemiology of C. hepaticum. Additionally in this study, the parts of the small subunit rRNA (18S rRNA) gene of Aonchotheca annulosa from bank vole, Aonchotheca erinacei and Eucoleus sp...
December 20, 2017: Acta Parasitologica
https://www.readbyqxmd.com/read/29035858/detection-of-small-number-of-giardia-in-biological-materials-prepared-from-stray-dogs
#17
Leila Esmailikia, Elahe Ebrahimzade, Parviz Shayan, Narges Amininia
Giardia lamblia is an intestinal protozoa with intermittent and low shedding especially in dogs, and the detection of Giardia is accompanied with problems such as sampling and diagnostic method. The objective of this study was to detection of Giardia in biological materials with low number of parasite using parasitological and molecular methods, and also to determine whether the examined stray dogs harbor known zoonotic genotype of Giardia. For this aim 85 fecal and duodenal samples were studied from which 1 was positive by Trichrome staining of stool, 4 were positive by staining of duodenal samples...
December 20, 2017: Acta Parasitologica
https://www.readbyqxmd.com/read/29035747/studies-on-the-adsorption-and-desorption-of-mitoxantrone-to-lauric-acid-albumin-coated-iron-oxide-nanoparticles
#18
Jan Zaloga, Artem Feoktystov, Vasil M Garamus, Weronika Karawacka, Alexander Ioffe, Thomas Brückel, Rainer Tietze, Christoph Alexiou, Stefan Lyer
A rational use of superparamagnetic iron oxide nanoparticles (SPIONs) in drug delivery, diagnostics, and other biomedical applications requires deep understanding of the molecular drug adsorption/desorption mechanisms for proper design of new pharmaceutical formulations. The adsorption and desorption of the cytostatic Mitoxantrone (MTO) to lauric acid-albumin hybrid coated particles SPIONs (SEON(LA-HSA)) was studied by Fourier transform infrared spectroscopy (FTIR), dynamic light scattering (DLS), surface titration, release experiments and small-angle neutron and X-ray scattering...
September 28, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29035554/star-polymers-reduce-iapp-toxicity-via-accelerated-amyloid-aggregation
#19
Emily H Pilkington, May Lai, Xinwei Ge, William J Stanley, Bo Wang, Miaoyi Wang, Aleksandr Kakinen, Marc-Antoine Sani, Michael R Whittaker, Esteban N Gurzov, Feng Ding, John F Quinn, Thomas P Davis, Pu Chun Ke
Protein aggregation into amyloid fibrils is a ubiquitous phenomenon across the spectrum of neurodegenerative disorders and type 2 diabetes. A common strategy against amyloidogenesis is to minimize the populations of toxic oligomers and protofibrils by inhibiting protein aggregation with small molecules or nanoparticles. However, melanin synthesis in nature is realized by accelerated protein fibrillation to circumvent accumulation of toxic intermediates. Accordingly, we designed and demonstrated the use of star-shaped poly(2-hydroxyethyl acrylate) (PHEA) nanostructures for promoting the aggregation while ameliorating the toxicity of human islet amyloid polypeptide (IAPP), the peptide involved in glycemic control and the pathology of type 2 diabetes...
October 16, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/29035546/the-gradient-adaptive-decomposition-grad-method-optimized-refinement-along-macrostate-borders-in-markov-state-models
#20
Pablo G Romano, Marina Giuseppina Guenza
Markov State Models (MSM) are used to model the kinetics of processes sampled by Molecular Dynamics (MD) simulations. MSM reduce the high dimensionality inherent to MD simulations as they partition the free energy landscape into discrete states, generating a kinetic model as a series of uncorrelated jumps between states. Here, we detail a new method, called GRadient Adaptive Decomposition, which optimizes coarse-grained MSM by refining borders with respect to the gradient along the free energy surface. The proposed method requires only a small number of initial microstates because it corrects for errors produced by limited sampling...
October 16, 2017: Journal of Chemical Information and Modeling
keyword
keyword
106614
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"