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Myosin IIB

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https://www.readbyqxmd.com/read/29773223/corrigendum-to-non-muscle-myosin-iia-and-iib-differentially-regulate-cell-shape-changes-during-zebrafish-brain-morphogenesis-dev-biol-397-2015-103-115
#1
Jennifer H Gutzman, Srishti U Sahu, Constance Kwas
No abstract text is available yet for this article.
May 14, 2018: Developmental Biology
https://www.readbyqxmd.com/read/29538357/pulmonary-and-diaphragmatic-pathology-in-collagen-type-i-%C3%AE-1-mutant-mice-with-osteogenesis-imperfecta
#2
Carolyn J Baglole, Feng Liang, Hussein Traboulsi, Angela Rico de Souza, Christian Giordano, Josephine T Tauer, Frank Rauch, Basil J Petrof
BackgroundOsteogenesis imperfecta (OI) is most often caused by mutations in type I collagen genes. Respiratory complications have been largely attributed to spine and ribcage deformities. We hypothesized that direct involvement of the pulmonary parenchyma and/or diaphragm by the disease may occur.MethodsIn Col1a1Jrt/+ mice, a model of severe dominant OI, mean linear intercept length (Lm) was used to assess the distal airspace size. Cross-sectional area (CSA) and myosin heavy chain (MyHC) phenotype of the diaphragm muscle fibers, as well as contractile properties, were determined...
May 9, 2018: Pediatric Research
https://www.readbyqxmd.com/read/29522098/cardiomyocyte-binucleation-is-associated-with-aberrant-mitotic-microtubule-distribution-mislocalization-of-rhoa-and-iqgap3-as-well-as-defective-actomyosin-ring-anchorage-and-cleavage-furrow-ingression
#3
Marina Leone, Gentian Musa, Felix Benedikt Engel
Aims: After birth mammalian cardiomyocytes initiate a last cell cycle which results in binucleation due to cytokinesis failure. Despite its importance for cardiac regenerative therapies, this process is poorly understood. Here, we aimed at a better understanding of the difference between cardiomyocyte proliferation and binucleation, and providing a new tool to distinguish these two processes. Methods and Results: Monitoring of cell division by time-lapse imaging revealed that rat cardiomyocyte binucleation stems from a failure to properly ingress the cleavage furrow...
March 7, 2018: Cardiovascular Research
https://www.readbyqxmd.com/read/29486156/nonmuscle-myosin-iia-and-iib-differentially-contribute-to-intrinsic-and-directed-migration-of-human-embryonic-lung-fibroblasts
#4
Masahiro Kuragano, Yota Murakami, Masayuki Takahashi
Nonmuscle myosin II (NMII) plays an essential role in directional cell migration. In this study, we investigated the roles of NMII isoforms (NMIIA and NMIIB) in the migration of human embryonic lung fibroblasts, which exhibit directionally persistent migration in an intrinsic manner. NMIIA-knockdown (KD) cells migrated unsteadily, but their direction of migration was approximately maintained. By contrast, NMIIB-KD cells occasionally reversed their direction of migration. Lamellipodium-like protrusions formed in the posterior region of NMIIB-KD cells prior to reversal of the migration direction...
March 25, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29467250/different-contributions-of-nonmuscle-myosin-iia-and-iib-to-the-organization-of-stress-fiber-subtypes-in-fibroblasts
#5
Masahiro Kuragano, Taro Q P Uyeda, Keiju Kamijo, Yota Murakami, Masayuki Takahashi
We demonstrated that myosin IIA and IIB are essential for the formation of transverse arcs and ventral stress fibers, respectively. Furthermore, we illustrated the roles of both isoforms in lamellar flattening and also raised the possibility that actin filaments in ventral stress fibers are in a stretched conformation.
April 15, 2018: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/29291007/alteration-of-muscle-fiber-characteristics-and-the-ampk-sirt1-pgc-1%C3%AE-axis-in-skeletal-muscle-of-growing-pigs-fed-low-protein-diets-with-varying-branched-chain-amino-acid-ratios
#6
Yehui Duan, Fengna Li, Wenlong Wang, Qiuping Guo, Chaoyue Wen, Yulong Yin
There mainly exists four major myosin heavy chains (MyHC) (i.e., I, IIa, IIx, and IIb) in growing pigs. The current study aimed to explore the effects of low-protein diets supplemented with varying branched-chain amino acids (BCAAs) on muscle fiber characteristics and the AMPK-SIRT1-PGC-1α axis in skeletal muscles. Forty growing pigs (9.85 ± 0.35 kg) were allotted to 5 groups and fed with diets supplemented with varying leucine: isoleucine: valine ratios: 1:0.51:0.63 (20% crude protein, CP), 1:1:1 (17% CP), 1:0...
December 5, 2017: Oncotarget
https://www.readbyqxmd.com/read/29243257/differential-impact-of-tongue-exercise-on-intrinsic-lingual-muscles
#7
Miranda J Cullins, Brittany N Krekeler, Nadine P Connor
OBJECTIVES/HYPOTHESIS: Aging is associated with muscle fiber hypotrophy and decreased percentages of rapidly contracting myosin heavy chain (MyHC) type IIb muscle fibers. Tongue exercise programs used to treat dysphagia target age-related decline in tongue muscle function, but the impact of exercise on the intrinsic tongue muscles is unknown. We hypothesized that exercise would induce muscle fiber hypertrophy and increase the percentage of MyHC IIa fibers in the intrinsic tongue. STUDY DESIGN: Animal model...
December 15, 2017: Laryngoscope
https://www.readbyqxmd.com/read/29207028/role-of-the-cofilin-2-gene-in-regulating-the-myosin-heavy-chain-genes-in-mouse-myoblast-c2c12-cells
#8
Hongyan Zhu, Huixin Yang, Song Zhao, Junfeng Zhang, Dan Liu, Yumin Tian, Zhiyi Shen, Yuhong Su
The cofilin 2 (CFL2) and myosin heavy chain (MyHC) genes play a key role in muscle development and myofibrillar formation. The aim of the present study was to investigate the effect of CFL2 on genes involved in fiber formation and the mechanisms underlying this process. Undifferentiated and differentiated C2C12 cells (UDT and DT, respectively) were transfected with CFL2 small interfering RNA (siRNA). CFL2 mRNA and protein levels were assessed using reverse transcription polymerase chain reaction (RT-PCR) and western blotting, respectively...
February 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29175377/soft-drug-resistant-ovarian-cancer-cells-migrate-via-two-distinct-mechanisms-utilizing-myosin-ii-based-contractility
#9
Aastha Kapoor, Amlan Barai, Bhushan Thakur, Alakesh Das, Sejal R Patwardhan, Melissa Monteiro, Snehal Gaikwad, Amirali B Bukhari, Pankaj Mogha, Abhijit Majumder, Abhijit De, Pritha Ray, Shamik Sen
The failure of chemotherapeutic drugs in treatment of various cancers is attributed to the acquisition of drug resistance. However, the migration mechanisms of drug-resistant cancer cells remain incompletely understood. Here we address this question from a biophysical perspective by mapping the phenotypic alterations in ovarian cancer cells (OCCs) resistant to cisplatin and paclitaxel. We show that cisplatin-resistant (CisR), paclitaxel-resistant (PacR) and dual drug-resistant (i.e., resistant to both drugs) OCCs are more contractile and softer than drug-sensitive cells...
February 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29103954/catenins-steer-cell-migration-via-stabilization-of-front-rear-polarity
#10
Vassil Vassilev, Anna Platek, Sylvain Hiver, Hideki Enomoto, Masatoshi Takeichi
Cell migration plays a pivotal role in morphogenetic and pathogenetic processes. To achieve directional migration, cells must establish a front-to-rear axis of polarity. Here we show that components of the cadherin-catenin complex function to stabilize this front-rear polarity. Neural crest and glioblastoma cells undergo directional migration in vivo or in vitro. During this process, αE-catenin accumulated at lamellipodial membranes and then moved toward the rear with the support of a tyrosine-phosphorylated β-catenin...
November 20, 2017: Developmental Cell
https://www.readbyqxmd.com/read/29084269/non-muscle-myosin-iib-myh10-is-required-for-epicardial-function-and-coronary-vessel-formation-during-mammalian-development
#11
Liam A Ridge, Karen Mitchell, Ali Al-Anbaki, Wasay Mohiuddin Shaikh Qureshi, Louise A Stephen, Gennadiy Tenin, Yinhui Lu, Irina-Elena Lupu, Christopher Clowes, Abigail Robertson, Emma Barnes, Jayne A Wright, Bernard Keavney, Elisabeth Ehler, Simon C Lovell, Karl E Kadler, Kathryn E Hentges
The coronary vasculature is an essential vessel network providing the blood supply to the heart. Disruptions in coronary blood flow contribute to cardiac disease, a major cause of premature death worldwide. The generation of treatments for cardiovascular disease will be aided by a deeper understanding of the developmental processes that underpin coronary vessel formation. From an ENU mutagenesis screen, we have isolated a mouse mutant displaying embryonic hydrocephalus and cardiac defects (EHC). Positional cloning and candidate gene analysis revealed that the EHC phenotype results from a point mutation in a splice donor site of the Myh10 gene, which encodes NMHC IIB...
October 2017: PLoS Genetics
https://www.readbyqxmd.com/read/29057943/high-fat-diet-induced-insulin-resistance-in-single-skeletal-muscle-fibers-is-fiber-type-selective
#12
Mark W Pataky, Haiyan Wang, Carmen S Yu, Edward B Arias, Robert J Ploutz-Snyder, Xiaohua Zheng, Gregory D Cartee
Skeletal muscle is the major site for insulin-stimulated glucose disposal, and muscle insulin resistance confers many negative health outcomes. Muscle is composed of multiple fiber types, and conventional analysis of whole muscles cannot elucidate fiber type differences at the cellular level. Previous research demonstrated that a brief (two weeks) high fat diet (HFD) caused insulin resistance in rat skeletal muscle. The primary aim of this study was to determine in rat skeletal muscle the influence of a brief (two weeks) HFD on glucose uptake (GU) ± insulin in single fibers that were also characterized for fiber type...
October 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29049414/wavelet-imaging-on-multiple-scales-wims-reveals-focal-adhesion-distributions-dynamics-and-coupling-between-actomyosin-bundle-stability
#13
Tim Toplak, Benoit Palmieri, Alba Juanes-García, Miguel Vicente-Manzanares, Martin Grant, Paul W Wiseman
We introduce and use Wavelet Imaging on Multiple Scales (WIMS) as an improvement to fluorescence correlation spectroscopy to measure physical processes and features that occur across multiple length scales. In this study, wavelet transforms of cell images are used to characterize molecular dynamics at the cellular and subcellular levels (i.e. focal adhesions). We show the usefulness of the technique by applying WIMS to an image time series of a migrating osteosarcoma cell expressing fluorescently labelled adhesion proteins, which allows us to characterize different components of the cell ranging from optical resolution scale through to focal adhesion and whole cell size scales...
2017: PloS One
https://www.readbyqxmd.com/read/28961272/sorbin-and-sh3-domain-containing-protein-2-sorbs2-is-a-component-of-the-acto-myosin-ring-at-the-apical-junctional-complex-in-epithelial-cells
#14
Karin Fredriksson-Lidman, Christina M Van Itallie, Amber J Tietgens, James M Anderson
SORBS2 is a scaffolding protein associated with Abl/Arg non-receptor tyrosine kinase pathways and is known to interact with actin and several other cytoskeletal proteins in various cell types. Previous BioID proximity labeling of tight and adherens junction proteins suggested that SORBS2 is a component of the apical junction complex of epithelial cells. We asked whether SORBS2 plays a previously unappreciated role in controlling perijunctional actin and tight junction barrier function. Using super resolution imaging we confirmed that SORBS2 is localized at the apical junction complex but farther from the membrane than ZO-1 and located partially overlapping both the tight- and adherens junctions with a periodic concentration that alternates with myosin IIB in polarized epithelial cells...
2017: PloS One
https://www.readbyqxmd.com/read/28912360/influence-of-past-injurious-exercise-on-fiber-type-specific-acute-anabolic-response-to-resistance-exercise-in-skeletal-muscle
#15
Ryo Takagi, Riki Ogasawara, Junya Takegaki, Arata Tsutaki, Koichi Nakazato, Naokata Ishii
We investigated the influence of past injurious exercise on anabolic response of skeletal muscle fibers to resistance exercise (RE). Wistar rats were divided into exercise (E) and exercise-after-injury (I-E) groups. At age 10 wk, the right gastrocnemius muscle in each rat in the I-E group was subjected to strenuous eccentric contractions. Subsequently, RE was imposed on the same muscle of each rat at 14 wk of age in both groups. Peak joint torque and total force generation per body mass during RE were similar between the groups...
January 1, 2018: Journal of Applied Physiology
https://www.readbyqxmd.com/read/28892032/semi-automated-analysis-of-mouse-skeletal-muscle-morphology-and-fiber-type-composition
#16
Sidharth Tyagi, Donald Beqollari, Chang Seok Lee, Lori A Walker, Roger A Bannister
For years, distinctions between skeletal muscle fiber types were best visualized by myosin-ATPase staining. More recently, immunohistochemical staining of myosin heavy chain (MyHC) isoforms has emerged as a finer discriminator of fiber-type. Type I, type IIA, type IIX and type IIB fibers can now be identified with precision based on their MyHC profile; however, manual analysis of these data can be slow and down-right tedious. In this regard, rapid, accurate assessment of fiber-type composition and morphology is a very desirable tool...
August 31, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28861647/fiber-type-distribution-and-expression-of-myosin-heavy-chain-isoforms-in-newborn-heterozygous-myostatin-knockout-pigs
#17
Xiao-Xu Xing, Mei-Fu Xuan, Long Jin, Qing Guo, Zhao-Bo Luo, Jun-Xia Wang, Qi-Rong Luo, Guang-Lei Zhang, Cheng-Du Cui, Zheng-Yun Cui, Jin-Dan Kang, Xi-Jun Yin
OBJECTIVES: To explore the effects of heterozygous myostatin-knockout (MSNT(+/-)) on muscle characteristics, specifically fiber-type distribution and expression of myosin heavy chain isoforms in pigs. RESULTS: The fiber cross-sectional area of the semitendinosus and semimembranosus muscles were much larger in MSTN(+/-) pigs at birth than in wild-type (WT) pigs. MSTN(+/-) pigs had a higher proportion of fast-type fibers and lower succinate dehydrogenase activity in muscles than WT pigs...
August 31, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/28823135/the-meat-quality-muscle-fiber-characteristics-and-fatty-acid-profile-in-jinjiang-and-f1-simmental%C3%A3-jinjiang-yellow-cattle
#18
Yue Zheng, Shizhi Wang, Peishi Yan
OBJECTIVE: This study compared the meat quality, muscle fiber characteristics, and fatty acids between Jinjiang yellow cattle (JJ) and F1 Simmental×Jinjiang yellow cattle (SJ) which were offered the same diet. METHODS: Six JJ and six SJ individuals were reared and fattened from 10 to 26 months of age. After feeding, the highrib (HR), ribeye (RB), and tenderloin (TL) samples were taken from the carcass for meat quality evaluations. RESULTS: The results showed that growth performance of SJ was higher than that of JJ (higher live weight and average daily gain), and the hot carcass weight of SJ was higher than that of JJ (p<0...
February 2018: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28821574/mosaic-loss-of-non-muscle-myosin-iia-and-iib-is-sufficient-to-induce-mammary-epithelial-proliferation
#19
Kim-Vy Nguyen-Ngoc, Vanesa L Silvestri, Dan Georgess, Amanda N Fairchild, Andrew J Ewald
The mammary epithelium elaborates through hormonally regulated changes in proliferation, migration and differentiation. Non-muscle myosin II (NMII) functions at the interface between contractility, adhesion and signal transduction. It is therefore a plausible regulator of mammary morphogenesis. We tested the genetic requirement for NMIIA and NMIIB in mammary morphogenesis through deletion of the three NMII heavy chain-encoding genes ( NMHCIIA , NMHCIIB and NMHCIIC ; also known as MYH9 , MYH10 and MYH14 , respectively) that confer specificity to the complex...
October 1, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28814507/systematic-gene-tagging-using-crispr-cas9-in-human-stem-cells-to-illuminate-cell-organization
#20
Brock Roberts, Amanda Haupt, Andrew Tucker, Tanya Grancharova, Joy Arakaki, Margaret A Fuqua, Angelique Nelson, Caroline Hookway, Susan A Ludmann, Irina A Mueller, Ruian Yang, Rick Horwitz, Susanne M Rafelski, Ruwanthi N Gunawardane
We present a CRISPR/Cas9 genome-editing strategy to systematically tag endogenous proteins with fluorescent tags in human induced pluripotent stem cells (hiPSC). To date, we have generated multiple hiPSC lines with monoallelic green fluorescent protein tags labeling 10 proteins representing major cellular structures. The tagged proteins include alpha tubulin, beta actin, desmoplakin, fibrillarin, nuclear lamin B1, nonmuscle myosin heavy chain IIB, paxillin, Sec61 beta, tight junction protein ZO1, and Tom20...
October 15, 2017: Molecular Biology of the Cell
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