keyword
MENU ▼
Read by QxMD icon Read
search

Myosin IIB

keyword
https://www.readbyqxmd.com/read/28538157/cell-and-tissue-scale-forces-coregulate-fgfr2-dependent-tetrads-and-rosettes-in-the-mouse-embryo
#1
Jun Wen, Hirotaka Tao, Kimberly Lau, Haijiao Liu, Craig A Simmons, Yu Sun, Sevan Hopyan
What motivates animal cells to intercalate is a longstanding question that is fundamental to morphogenesis. A basic mode of cell rearrangement involves dynamic multicellular structures called tetrads and rosettes. The contribution of cell-intrinsic and tissue-scale forces to the formation and resolution of these structures remains unclear, especially in vertebrates. Here, we show that Fgfr2 regulates both the formation and resolution of tetrads and rosettes in the mouse embryo, possibly in part by spatially restricting atypical protein kinase C, a negative regulator of non-muscle myosin IIB...
May 23, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28533420/igf1-stimulates-greater-muscle-hypertrophy-in-the-absence-of-myostatin-in-male-mice
#2
Alexander Hennebry, Jenny M Oldham, Tea Shavlakadze, Miranda Grounds, Philip Sheard, Marta Fiorotto, Shelley J Falconer, Heather K Smith, Carole Berry, Ferenc Jeanplong, Jeremy Bracegirdle, Kenneth G Matthews, Gina Nicholas, Monica Senna-Salerno, Trevor Watson, Christopher D McMahon
Insulin-like growth factors (IGFs) and myostatin have opposing roles in regulating the growth and size of skeletal muscle, with IGF1 stimulating, and myostatin inhibiting, growth. However, it remains unclear whether these proteins have mutually dependent, or independent, roles. To clarify this issue, we crossed myostatin null (Mstn-/-) mice with mice over-expressing Igf1 in skeletal muscle (Igf1+) to generate six genotypes of male mice; wild-type (Mstn+/+), Mstn+/-, Mstn-/-, Mstn+/+:Igf1+, Mstn+/-:Igf1+ and Mstn-/-:Igf1+...
May 22, 2017: Journal of Endocrinology
https://www.readbyqxmd.com/read/28475953/effects-of-dietary-starch-types-on-growth-performance-meat-quality-and-myofibre-type-of-finishing-pigs
#3
Y J Li, J L Li, L Zhang, F Gao, G H Zhou
To investigate the effects of dietary starch types on growth performance and meat quality of finishing pigs, ninety barrows (68.0±2.0kg) were randomly allotted to three experimental diets with five replicates of six pigs, containing pure waxy maize starch (WMS), nonwaxy maize starch (NMS) and pea starch (PS) (amylose/amylopectin were 0.07, 0.19 and 0.28 respectively). Compared with WMS diet, PS diet increased the average daily gain, loin eye area, pH45 value, NMR transverse relaxation (T2)2 peak area ratio and sarcoplasmic protein solubility, decreased the feed to gain ratio, back fat, drip loss, cooking loss and T23 peak area ratio (P<0...
May 2, 2017: Meat Science
https://www.readbyqxmd.com/read/28431393/loss-of-tpm4-1-leads-to-disruption-of-cell-cell-adhesions-and-invasive-behavior-in-breast-epithelial-cells-via-increased-rac1-signaling
#4
SukYeong Jeong, SunYoung Lim, Galina Schevzov, Peter W Gunning, David M Helfman
Here we report the identification and characterization of a novel high molecular weight isoform of tropomyosin, Tpm4.1, expressed from the human TPM4 gene. Tpm4.1 expression is down-regulated in a subset of breast cancer cells compared with untransformed MCF10A breast epithelial cells and in highly metastatic breast cancer cell lines derived from poorly metastatic MDA-MD-231 cells. In addition, patients with invasive ductal breast carcinoma show decreased TPM4 expression compared with patients with ductal breast carcinoma in situ, and low TPM4 expression is associated with poor prognosis...
May 16, 2017: Oncotarget
https://www.readbyqxmd.com/read/28352215/myosin-iia-related-actomyosin-contractility-mediates-oxidative-stress-induced-neuronal-apoptosis
#5
Yan Wang, Yingqiong Xu, Qian Liu, Yuanyuan Zhang, Zhen Gao, Mingzhu Yin, Nan Jiang, Guosheng Cao, Boyang Yu, Zhengyu Cao, Junping Kou
Oxidative stress-induced neuronal apoptosis plays an important role in the progression of central nervous system (CNS) diseases. In our study, when neuronal cells were exposed to hydrogen peroxide (H2O2), an exogenous oxidant, cell apoptosis was observed with typical morphological changes including membrane blebbing, neurite retraction and cell contraction. The actomyosin system is considered to be responsible for the morphological changes, but how exactly it regulates oxidative stress-induced neuronal apoptosis and the distinctive functions of different myosin II isoforms remain unclear...
2017: Frontiers in Molecular Neuroscience
https://www.readbyqxmd.com/read/28345668/c9orf135-encodes-a-membrane-protein-whose-expression-is-related-to-pluripotency-in-human-embryonic-stem-cells
#6
Shixin Zhou, Yinan Liu, Yumin Ma, Xiaoyan Zhang, Yang Li, Jinhua Wen
Human embryonic stem cells (hESCs) are a unique population of cells defined by their capacity for self-renewal and pluripotency. Here, we identified a previously uncharacterized gene in hESCs, C9ORF135, which is sharply downregulated during gastrulation and gametogenesis, along with the pluripotency factors OCT4, SOX2, and NANOG. Human ESCs express two C9ORF135 isoforms, the longer of which encodes a membrane-associated protein, as determined by immunostaining and western blotting of fractionated cell lysates...
March 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28345221/effect-of-timing-of-electromyostimulation-on-denervated-skeletal-muscle-atrophy
#7
Eun Sil Koh, Hee Chan Kim, Jae-Young Lim
INTRODUCTION: In this study we evaluated the effect of electromyostimulation (EMS) on myosin heavy chain (MHC) isoform expression in denervated rat muscles to determine the optimal timing for EMS application. METHODS: EMS was initiated on post-injury day 1 for the group with denervation receiving immediate EMS (DIEMS) and on post-injury day 15 for the group with denervation receiving delayed EMS (DDEMS) in rat denervated muscles. Muscle wet weight and muscle fiber cross-sectional area (FCSA) were measured...
March 27, 2017: Muscle & Nerve
https://www.readbyqxmd.com/read/28328100/smart-mechanosensing-machineries-enable-migration-of-vascular-smooth-muscle-cells-in-atherosclerosis-relevant-3d-matrices
#8
REVIEW
Qingjia Chi, Jieling Shan, Xiaorong Ding, Tieying Yin, Yazhou Wang, Dongyu Jia, Guixue Wang
At the early stage of atherosclerosis, neointima is formed due to the migration of vascular smooth muscle cells (VSMCs) from the media to the intima. VSMCs are surrounded by highly adhesive 3D matrices. They take specific strategies to cross various 3D matrices in the media, including heterogeneous collagen and mechanically strong basement membrane. Migration of VSMCs is potentially caused by biomechanical mechanism. Most in vitro studies focus on cell migration on 2D substrates in response to biochemical factors...
March 22, 2017: Cell Biology International
https://www.readbyqxmd.com/read/28251924/differential-role-of-nonmuscle-myosin-ii-isoforms-during-blebbing-of-mcf-7-cells
#9
Sumit K Dey, Raman K Singh, Shyamtanu Chattoraj, Shekhar Saha, Alakesh Das, Kankan Bhattacharyya, Kaushik Sengupta, Shamik Sen, Siddhartha S Jana
Bleb formation has been correlated with nonmuscle myosin II (NM-II) activity. Whether three isoforms of NM-II (NM-IIA, -IIB and -IIC) have the same or differential roles in bleb formation is not well understood. Here we report that ectopically expressed, GFP-tagged NM-II isoforms exhibit different types of membrane protrusions, such as multiple blebs, lamellipodia, combinations of both, or absence of any such protrusions in MCF-7 cells. Quantification suggests that 50% of NM-IIA-GFP-, 29% of NM-IIB-GFP-, and 19% of NM-IIC1-GFP-expressing MCF-7 cells show multiple bleb formation, compared with 36% of cells expressing GFP alone...
April 15, 2017: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/28228547/limch1-regulates-nonmuscle-myosin-ii-activity-and-suppresses-cell-migration
#10
Yu-Hung Lin, Yen-Yi Zhen, Kun-Yi Chien, I-Ching Lee, Wei-Chi Lin, Mei-Yu Chen, Li-Mei Pai
Nonmuscle myosin II (NM-II) is an important motor protein involved in cell migration. Incorporation of NM-II into actin stress fiber provides a traction force to promote actin retrograde flow and focal adhesion assembly. However, the components involved in regulation of NM-II activity are not well understood. Here we identified a novel actin stress fiber-associated protein, LIM and calponin-homology domains 1 (LIMCH1), which regulates NM-II activity. The recruitment of LIMCH1 into contractile stress fibers revealed its localization complementary to actinin-1...
April 15, 2017: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/28214291/segmental-distribution-of-myosin-heavy-chain-isoforms-within-single-muscle-fibers
#11
Ming Zhang, Maree Gould
Despite many studies looking at the distribution of myosin heavy chain (MHC) isoforms across a transverse section of muscle, knowledge of MHC distribution along the longitudinal axis of a single skeletal muscle fiber has been relatively overlooked. Immunocytochemistry was performed on serial sections of rat extensor digitorum longus (EDL) muscle to identify MHC types I, IIA, IIX, IIY and IIB. Sixteen fascicles which contained a total of 362 fibers were randomly and systematically sampled from the 3 EDL muscles...
February 18, 2017: Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology
https://www.readbyqxmd.com/read/28209124/rna-seq-and-metabolomic-analyses-of-akt1-mediated-muscle-growth-reveals-regulation-of-regenerative-pathways-and-changes-in-the-muscle-secretome
#12
Chia-Ling Wu, Yoshinori Satomi, Kenneth Walsh
BACKGROUND: Skeletal muscle is a major regulator of systemic metabolism as it serves as the major site for glucose disposal and the main reservoir for amino acids. With aging, cachexia, starvation, and myositis, there is a preferential loss of fast glycolytic muscle fibers. We previously reported a mouse model in which a constitutively-active Akt transgene is induced to express in a subset of muscle groups leading to the hypertrophy of type IIb myofibers with an accompanying increase in strength...
February 16, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28181366/molecular-phenotype-and-bleeding-risks-of-an-inherited-platelet-disorder-in-a-family-with-a-runx1-frameshift-mutation
#13
M S Badin, J K Iyer, M Chong, L Graf, G E Rivard, J S Waye, A D Paterson, G Pare, C P M Hayward
INTRODUCTION: Inherited defects in RUNX1 are important causes of platelet function disorders. AIM: Our goals were to evaluate RUNX1-related platelet disorders among individuals evaluated for uncharacterized, inherited platelet function disorders and test a proof of concept that bleeding risks could be quantitatively estimated for typical families with an inherited platelet function disorder. METHODS: Index cases with an uncharacterized inherited platelet function disorder were subjected to exome sequencing with confirmation of RUNX1 mutations by Sanger sequencing...
February 8, 2017: Haemophilia: the Official Journal of the World Federation of Hemophilia
https://www.readbyqxmd.com/read/28097658/segmental-fibre-type-composition-of-the-rat-iliopsoas-muscle
#14
Hrvoje Vlahovic, Ksenija Bazdaric, Verner Marijancic, Tamara Soic-Vranic, Daniela Malnar, Juraj Arbanas
The iliopsoas of the rat is composed of two muscles - the psoas major muscle and the iliacus muscle. The psoas major muscle arises from all the lumbar vertebrae and the iliacus muscle from the fifth and sixth lumbar vertebrae and ilium. Their common insertion point is the lesser trochanter of the femur, and their common action is the lateral rotation of the femur and flexion of the hip joint. Unlike humans, the rat is a quadruped and only occasionally rises up on its hind legs. Therefore, it is expected that the fibre type composition of the rat iliopsoas muscle will be different than that of humans...
April 2017: Journal of Anatomy
https://www.readbyqxmd.com/read/28074127/transfer-of-%C3%AE-hydroxy-%C3%AE-methylbutyrate-from-sows-to-their-offspring-and-its-impact-on-muscle-fiber-type-transformation-and-performance-in-pigs
#15
Haifeng Wan, Jiatao Zhu, Caimei Wu, Pan Zhou, Yong Shen, Yan Lin, Shengyu Xu, Lianqiang Che, Bin Feng, Jian Li, Zhengfeng Fang, De Wu
BACKGROUND: Previous studies suggested that supplementation of lactating sows with β-hydroxy-β-methylbutyrate (HMB) could improve the performance of weaning pigs, but there were little information in the muscle fiber type transformation of the offspring and the subsequent performance in pigs from weaning through finishing in response to maternal HMB consumption. The purpose of this study was to determine the effect of supplementing lactating sows with HMB on skeletal muscle fiber type transformation and growth of the offspring during d 28 and 180 after birth...
2017: Journal of Animal Science and Biotechnology
https://www.readbyqxmd.com/read/28018235/modulation-of-muscle-fiber-compositions-in-response-to-hypoxia-via-pyruvate-dehydrogenase-kinase-1
#16
Daniel D Nguyen, Gyuyoup Kim, Eung-Kwon Pae
Muscle fiber-type changes in hypoxic conditions in accordance with pyruvate dehydrogenase kinase (Pdk)-1 and hypoxia inducible factor (Hif)-1α were investigated in rats. Hif-1α and its down-stream molecule Pdk-1 are well known for readily response to hypoxia. We questioned their roles in relation to changes in myosin heavy chain (MyHC) composition in skeletal muscles. We hypothesize that the level of Pdk-1 with respect to the level of Hif-1α determines MyHC composition of the muscle in rats in hypoxia. Young male rats were housed in a chamber maintained at 11...
2016: Frontiers in Physiology
https://www.readbyqxmd.com/read/28017710/a-vitamin-d-analogue-eldecalcitol-enhances-expression-of-fast-myosin-heavy-chain-subtypes-in-differentiated-c2c12-myoblasts
#17
Hideo Saito, Koshi N Kishimoto, Yu Mori, Hiroshi Okuno, Masahiko Tanaka, Eiji Itoi
BACKGROUND: Several lines of evidence indicate that the active form of vitamin D has an anabolic effect on skeletal muscle. Eldecalcitol, an analogue of the active form of vitamin D, has the potential to increase bone density and decrease fracture risk. The objective of this study was to investigate the effect of eldecalcitol in C2C12 myogenic cells. METHODS: C2C12 cells were grown to confluency and the culture medium was replaced with low-glucose DMEM containing 2% horse serum...
December 23, 2016: Journal of Orthopaedic Science: Official Journal of the Japanese Orthopaedic Association
https://www.readbyqxmd.com/read/27960114/identification-and-quantification-of-myosin-heavy-chain-isoforms-in-bovine-and-porcine-longissimus-muscles-by-lc-ms-ms-analysis
#18
Gap-Don Kim, Tae-Chul Jeong, Kye Man Cho, Jin-Yeon Jeong
To identify and quantify the myosin heavy chain (MHC) isoforms in porcine and bovine longissimus thoracis (LT) muscles, LC-MS/MS and label-free quantification were conducted. 2a, 2x, 2b and slow isoforms were identified in porcine LT muscle, whereas bovine LT muscle contained 2a, 2x and slow isoforms. The highest peak intensity (44.32%) of MHC 2b was observed in porcine LT muscle, whereas the peak intensity of MHC 2a was the highest (71.15%) in bovine LT muscle (p<0.05). MHC-based fiber compositions for porcine and bovine LT were also the highest in types IIB (61...
March 2017: Meat Science
https://www.readbyqxmd.com/read/27897407/activin-a-induces-skeletal-muscle-catabolism-via-p38%C3%AE-mitogen-activated-protein-kinase
#19
Hui Ding, Guohua Zhang, Ka Wai Thomas Sin, Zhelong Liu, Ren-Kuo Lin, Min Li, Yi-Ping Li
BACKGROUND: Activation of type IIB activin receptor (ActRIIB) in skeletal muscle leads to muscle atrophy because of increased muscle protein degradation. However, the intracellular signalling mechanism that mediates ActRIIB-activated muscle catabolism is poorly defined. METHODS: We investigated the role of p38β mitogen-activated protein kinases (MAPK) in mediating ActRIIB ligand activin A-activated muscle catabolic pathways in C2C12 myotubes and in mice with perturbation of this kinase pharmacologically and genetically...
April 2017: Journal of Cachexia, Sarcopenia and Muscle
https://www.readbyqxmd.com/read/27870829/crumbs2-promotes-cell-ingression-during-the-epithelial-to-mesenchymal-transition-at-gastrulation
#20
Nitya Ramkumar, Tatiana Omelchenko, Nancy F Silva-Gagliardi, C Jane McGlade, Jan Wijnholds, Kathryn V Anderson
During gastrulation of the mouse embryo, individual cells ingress in an apparently stochastic pattern during the epithelial-to-mesenchymal transition (EMT). Here we define a critical role of the apical protein Crumbs2 (CRB2) in the gastrulation EMT. Static and live imaging show that ingressing cells in Crumbs2 mutant embryos become trapped at the primitive streak, where they continue to express the epiblast transcription factor SOX2 and retain thin E-cadherin-containing connections to the epiblast surface that trap them at the streak...
December 2016: Nature Cell Biology
keyword
keyword
106543
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"