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https://www.readbyqxmd.com/read/28605645/novel-in-vivo-experimental-viability-assays-with-high-sensitivity-and-throughput-capacity-using-a-bdelloid-rotifer
#1
Zita Olah, Ashley I Bush, David Aleksza, Bence Galik, Eszter Ivitz, Lilla Macsai, Zoltan Janka, Zoltan Karman, Janos Kalman, Zsolt Datki
Rotifers have been used in biological research as well-characterized models of aging. Their multi-organ characters and their sensitivity for chemicals and environmental changes make them useful as in vivo toxicological and lifespan models. Our aim was to create a bdelloid rotifer model to use in high-throughput viability and non-invasive assays. In order to identify our species Philodina acuticornis odiosa (PA), 18S rDNA-based phylogenetic analysis was carried out and their species-specific morphological markers identified...
June 9, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28550991/production-of-1-3-diols-in-escherichia-coli
#2
Naoya Kataoka, Alisa S Vangnai, Thunyarat Pongtharangkul, Toshiharu Yakushi, Kazunobu Matsushita
To expand the diversity of chemical compounds produced through microbial conversion, a platform pathway for the production of widely used industrial chemicals, 1,3-diols, was engineered in Escherichia coli. The pathway was designed by modifying the previously reported (R)-1,3-butanediol synthetic pathway to consist of pct (propionate CoA-transferase) from Megasphaera elsdenii, bktB (thiolase), phaB (NADPH-dependent acetoacetyl-CoA reductase) from Ralstonia eutropha, bld (butyraldehyde dehydrogenase) from Clostridium saccharoperbutylacetonicum, and the endogenous alcohol dehydrogenase(s) of E...
May 18, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28528473/properties-of-streptomyces-albus-j1074-mutant-deficient-in-trna-leu-uaa-gene-blda
#3
Oksana Koshla, Maria Lopatniuk, Ihor Rokytskyy, Oleksandr Yushchuk, Yuriy Dacyuk, Victor Fedorenko, Andriy Luzhetskyy, Bohdan Ostash
Streptomyces albus J1074 is one of the most popular and convenient hosts for heterologous expression of gene clusters directing the biosynthesis of various natural metabolic products, such as antibiotics. This fuels interest in elucidation of genetic mechanisms that may limit secondary metabolism in J1074. Here, we report the generation and initial study of J1074 mutant, deficient in gene bldA for tRNA(Leu)UAA, the only tRNA capable of decoding rare leucyl TTA codon in Streptomyces. The bldA deletion in J1074 resulted in a highly conditional Bld phenotype, with depleted formation of aerial hyphae on certain solid media...
May 20, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28476699/analysis-and-detection-of-microporosities-in-aging-bones-in-context-to-pharmaceutical-treatment-a-study-in-line-of-osteoporosis
#4
Nadeem Baig, Muhammad Zaheer Khan
The objective of the current study was to evaluate the effects of nandrolone decanoate in terms of morphological changes in the diaphyseal region of appendicular skeleton long bone (femur) of aging female Albino rats with the help of innovatively designed histomorphometric tool of study being introduced first time mapped out micro porosities (lacunae) which tend to intensify as part of advanced aging denoting deterioration in osseous tissue. In fact our research trial has been in the line of involutional osteoporosis especially common in post-menopausal aged women, as to find out appropriate drug usefulness in the care and cure of this diseased state as per recommendation of FDA...
September 2016: Pakistan Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28318793/fate-and-mass-balance-of-bisphenol-analogues-in-wastewater-treatment-plants-in-xiamen-city-china
#5
Qian Sun, Yuwen Wang, Yan Li, Muhammad Ashfaq, Lanhua Dai, Xiaoqing Xie, Chang-Ping Yu
Due to the well-known endocrine disrupting ability of bisphenol A (BPA), its production and usage have been regulated. Consequently, other bisphenol analogues (BPs) have been used as the replacement of BPA. Despite their widespread use, few studies have investigated the occurrence and fate of BPs in wastewater treatment plants (WWTPs). In this study, we conducted a city-wide monitoring survey and collected the wastewater and sludge samples from seven WWTPs in Xiamen, China over seven days. The concentrations of dissolved and adsorbed BPs in the influent and effluent, together with the BP concentrations in the sludge were determined...
June 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28293229/fungal-root-microbiome-from-healthy-and-brittle-leaf-diseased-date-palm-trees-phoenix-dactylifera-l-reveals-a-hidden-untapped-arsenal-of-antibacterial-and-broad-spectrum-antifungal-secondary-metabolites
#6
Fedia B Mefteh, Amal Daoud, Ali Chenari Bouket, Faizah N Alenezi, Lenka Luptakova, Mostafa E Rateb, Adel Kadri, Neji Gharsallah, Lassaad Belbahri
In this study, we aimed to explore and compare the composition, metabolic diversity and antimicrobial potential of endophytic fungi colonizing internal tissues of healthy and brittle leaf diseased (BLD) date palm trees (Phoenix dactylifera L.) widely cultivated in arid zones of Tunisia. A total of 52 endophytic fungi were isolated from healthy and BLD roots of date palm trees, identified based on internal transcribed spacer-rDNA sequence analysis and shown to represent 13 species belonging to five genera. About 36...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28167420/the-influence-of-passenger-car-front-shape-on-pedestrian-injury-risk-observed-from-german-in-depth-accident-data
#7
Guibing Li, Mathew Lyons, Bingyu Wang, Jikuang Yang, Dietmar Otte, Ciaran Simms
Quantified relationships between passenger car front shape and pedestrian injury risk derived from accident data are sparse, especially considering the significant recent changes in car front design. The purpose of this paper is therefore to investigate the detailed effects of passenger car front shape on injury risk to a pedestrian's head, thorax, pelvis and leg in the event of a vehicle pedestrian impact. Firstly, an accident sample of 594 pedestrian cases captured during 2000-2015 from the German In-Depth Accident Study (GIDAS) database was employed...
April 2017: Accident; Analysis and Prevention
https://www.readbyqxmd.com/read/28152499/contributions-of-polyunsaturated-fatty-acids-pufa-on-cerebral-neurobiology-an-integrated-omics-approach-with-epigenomic-focus
#8
Nabarun Chakraborty, Seid Muhie, Raina Kumar, Aarti Gautam, Seshamalini Srinivasan, Bintu Sowe, George Dimitrov, Stacy-Ann Miller, Marti Jett, Rasha Hammamieh
The epigenetic landscape is vulnerable to diets. Here, we investigated the influence of different polyunsaturated fatty acids (PUFA) dietary supplements on rodents' nervous system development and functions and potential consequences to neurodegenerative disorders. Our previous nutrigenomics study showed significant impact of high n-3 PUFA-enriched diet (ERD) on synaptogenesis and various neuromodulators. The present study introduced a second equicaloric diet with n-6 PUFA balanced by n-3 PUFA (BLD). The typical lab diet with high n-6 PUFA was the baseline...
January 5, 2017: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/28047251/tu-h-campus-jep2-02-interobserver-variability-of-ct-pet-ct-and-mri-based-primary-tumor-delineation-for-lung-cancer
#9
K Karki, G Hugo, S Saraiya, N Jan, J Schuster, M Schutzer, L Fahrner, R Groves, J Ford, E Weiss
PURPOSE: Target delineation in lung cancer radiotherapy has, in general, large variability. MRI has so far not been investigated in detail for lung cancer delineation variability. The purpose of this study is to investigate delineation variability for lung tumors using MRI and compare it to CT alone and PET-CT based delineations. METHODS: Seven physicians delineated the primary tumor volumes of nine patients for the following scenarios: (1) CT only; (2) post-contrast T1-weighted MRI registered with diffusion-weighted MRI; and (3) PET-CT fusion images...
June 2016: Medical Physics
https://www.readbyqxmd.com/read/27816968/diagnostic-and-prognostic-value-of-serum-periostin-in-patients-with-non-small-cell-lung-cancer
#10
Chun-Hua Xu, Wei Wang, Yong Lin, Li-Hua Qian, Xiu-Wei Zhang, Qing-Bo Wang, Li-Ke Yu
The periostin protein is expressed in a variety of human malignancies. The aim of this study was to explore the diagnostic and prognostic value of serum periostin levels in patients with non-small cell lung cancer (NSCLC). We measured serum periostin levels by ELISA in 296 NSCLC patients, 120 benign lung diseases (BLD) patients and 160 healthy controls. The levels of serum periostin in NSCLC patients were significantly elevated compared with those in healthy controls (P < 0.001) and BLD patients (P < 0...
March 21, 2017: Oncotarget
https://www.readbyqxmd.com/read/27721249/serum-carcinoembryonic-antigen-neuron-specific-enolase-as-biomarkers-for-diagnosis-of-nonsmall-cell-lung-cancer
#11
Yanjun Dong, Xianjie Zheng, Zhongxin Yang, Mingfei Sun, Guoyu Zhang, Xiaokang An, Lihong Pan, Shuanglin Zhang
OBJECTIVE: To investigate the clinical efficacy of serum carcinoembryonic antigen (CEA) and neuron-specific enolase (NSE) as biomarkers for diagnosis of nonsmall cell lung cancer (NSCLC). MATERIAL AND METHODS: Forty-six cytology or pathology confirmed nonsmall cell lung patients and 33 cases of benign lung disease (BLD) were retrospective reviewed in our hospital from February 2013 to January 2016. The serum concentrations of CEA and NSE were measured by chemiluminescent assay...
October 2016: Journal of Cancer Research and Therapeutics
https://www.readbyqxmd.com/read/27685634/activation-of-necroptosis-in-human-and-experimental-cholestasis
#12
Marta B Afonso, Pedro M Rodrigues, André L Simão, Dimitry Ofengeim, Tânia Carvalho, Joana D Amaral, Maria M Gaspar, Helena Cortez-Pinto, Rui E Castro, Junying Yuan, Cecília M P Rodrigues
Cholestasis encompasses liver injury and inflammation. Necroptosis, a necrotic cell death pathway regulated by receptor-interacting protein (RIP) 3, may mediate cell death and inflammation in the liver. We aimed to investigate the role of necroptosis in mediating deleterious processes associated with cholestatic liver disease. Hallmarks of necroptosis were evaluated in liver biopsies of primary biliary cholangitis (PBC) patients and in wild-type and RIP3-deficient (RIP3(-/-)) mice subjected to common bile duct ligation (BDL)...
September 29, 2016: Cell Death & Disease
https://www.readbyqxmd.com/read/27658408/distribution-and-risk-assessment-of-metals-and-arsenic-contamination-in-man-made-ditch-sediments-with-different-land-use-types
#13
Mathieu Nsenga Kumwimba, Bo Zhu, Tao Wang, Diana Kavidia Muyembe
Ditches are subjected to a large input of nutrients, trace metals, and arsenic and the enhancement of sedimentation due to human activities. However, the influence of different types of land uses on the distribution and associated environmental risk of metals and arsenic in the Red purple Sichuan Basin remains largely unclear, which is needed for water management. This study was carried out to characterize metal/metalloid status in ditch sediments from different land uses. A total of 68 surface sediment samples (0-5 cm) were collected from open ditches distributed in different land use types, i...
December 2016: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/27628532/corticospinal-and-transcallosal-modulation-of-unilateral-and-bilateral-contractions-of-lower-limbs
#14
Jakob Škarabot, Ruben Perellón Alfonso, Neil Cronin, Jure Bon, Vojko Strojnik, Janne Avela
PURPOSE: Interhemispheric inhibition may play a role in the expression of bilateral deficit in force (BLD). This study investigated whether the degree of BLD is reflected in the nature of interhemispheric interaction during unilateral and bilateral contractions. METHODS: Subjects divided into three groups, 'bilateral' (n = 7), 'unilateral' (n = 5) and 'control' (n = 8), performed unilateral and bilateral maximal voluntary isometric knee extensions while receiving electrical stimulation, and transcranial magnetic stimulation of the target and ipsilateral muscles...
December 2016: European Journal of Applied Physiology
https://www.readbyqxmd.com/read/27616713/evaluation-of-eight-high-spatial-resolution-gridded-precipitation-products-in-adige-basin-italy-at-multiple-temporal-and-spatial-scales
#15
Zheng Duan, Junzhi Liu, Ye Tuo, Gabriele Chiogna, Markus Disse
This study provides a comprehensive evaluation of eight high spatial resolution gridded precipitation products in Adige Basin located in Italy within 45-47.1°N. The Adige Basin is characterized by a complex topography, and independent ground data are available from a network of 101 rain gauges during 2000-2010. The eight products include the Version 7 TRMM (Tropical Rainfall Measuring Mission) Multi-satellite Precipitation Analysis 3B42 product, three products from CMORPH (the Climate Prediction Center MORPHing technique), i...
December 15, 2016: Science of the Total Environment
https://www.readbyqxmd.com/read/27616707/dm-bld-differential-methylation-detection-using-a-hierarchical-bayesian-model-exploiting-local-dependency
#16
Xiao Wang, Jinghua Gu, Leena Hilakivi-Clarke, Robert Clarke, Jianhua Xuan
MOTIVATION: The advent of high-throughput DNA methylation profiling techniques has enabled the possibility of accurate identification of differentially methylated genes for cancer research. The large number of measured loci facilitates whole genome methylation study, yet posing great challenges for differential methylation detection due to the high variability in tumor samples. RESULTS: We have developed a novel probabilistic approach, D: ifferential M: ethylation detection using a hierarchical B: ayesian model exploiting L: ocal D: ependency (DM-BLD), to detect differentially methylated genes based on a Bayesian framework...
January 15, 2017: Bioinformatics
https://www.readbyqxmd.com/read/27497730/a-metabolic-profiling-analysis-of-the-nephrotoxicity-of-acyclovir-in-rats-using-ultra-performance-liquid-chromatography-mass-spectrometry
#17
Wenmin Xing, Lili Gu, Xinyue Zhang, Jiadong Xu, Hong Lu
Acyclovir (ACV) exposure is a common cause of acute kidney injury (AKI). The toxicity mechanism of ACV has always been a matter of debate. The present study investigated into the time-effect relationship and dose-effect relationship of ACV-induced nephrotoxicity in rats using metabonomics. Twenty-four rats were randomly divided into four groups: a 0.9% NaCl solution group, and 100, 300, and 600mg/kg ACV-treated groups; the ACV or vehicle solution was administered with a single intravenous injection. Urine was collected at different time periods (12h before administration, and 0-6h, 7-12h, and 13-24h after administration)...
September 2016: Environmental Toxicology and Pharmacology
https://www.readbyqxmd.com/read/27372741/carbon-catabolite-regulation-of-secondary-metabolite-formation-and-morphological-differentiation-in-streptomyces-coelicolor
#18
A Romero-Rodríguez, B Ruiz-Villafán, V H Tierrafría, R Rodríguez-Sanoja, S Sánchez
In the genus Streptomyces, carbon utilization is of significant importance for the expression of genes involved in morphological differentiation and antibiotic production. However, there is little information about the mechanism involved in these effects. In the present work, it was found that glucose exerted a suppressive effect on the Streptomyces coelicolor actinorhodin (Act) and undecylprodigiosin (Red) production, as well as in its morphological differentiation. Accordingly, using a high-density microarray approach in S...
November 2016: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/27282414/bladder-tumor-targeted-delivery-of-pro-apoptotic-peptide-for-cancer-therapy
#19
Hyun-Kyung Jung, Soyoun Kim, Rang-Woon Park, Jae-Yong Park, In-San Kim, Byungheon Lee
The overall prognosis of conventional chemotherapy for the treatment of bladder cancer is poor and reduction of its systemic side effects remains an unsolved issue. Targeted therapy for bladder cancer could improve therapeutic efficacy and reduce side effects. This study investigated a hybrid peptide (named Bld-1-KLA) composed of the CSNRDARRC peptide (Bld-1), which binds to bladder tumor cells, and the d-KLAKLAKKLAKLAK (KLA) peptide, which disrupts mitochondrial membrane and induces apoptotic cell death, as a bladder cancer-targeted therapeutic agent...
August 10, 2016: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/27216449/diagnosis-value-of-serum-soluble-b7-h4-expression-in-non-small-cell-lung-cancer
#20
Chun Hua Xu, Wei Wang, Yu Chao Wang, Yong Lin, Xiu Wei Zhang
INTRODUCTION: B7-H4, a member of the inhibitory B7 family, can restrain T cell proliferation, activation, cytokine secretion, and may be involved in immune evasion in cancer patients. OBJECTIVES: This aim of the study was to determine the expression level of soluble B7-H4 (sB7-H4) in circulation and to subsequently evaluate the clinical significance of circulating sB7-H4 in patients with non-small cell lung cancer (NSCLC). METHODS: Serum specimens from 128 patients with NSCLC, 100 healthy volunteers (HV), and 80 patients with benign lung diseases (BLD) were collected...
May 23, 2016: Clinical Respiratory Journal
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