keyword
MENU ▼
Read by QxMD icon Read
search

Myosin II

keyword
https://www.readbyqxmd.com/read/28914606/nanoscale-architecture-of-the-schizosaccharomyces-pombe-contractile-ring
#1
Nathan A McDonald, Abigail L Lind, Sarah E Smith, Rong Li, Kathleen Gould
The contractile ring is a complex molecular apparatus important for dividing many eukaryotic cells. Despite knowledge of its composition, the molecular architecture of the ring is not known. Here we applied super-resolution microscopy and FRET to determine the nanoscale spatial organization of Schizosaccharomyces pombe contractile ring components relative to the plasma membrane. As in other membrane-tethered actin structures, contractile ring proteins are stratified relative to the membrane. The lowest layer (0-80 nm) contains membrane-binding scaffolds, formin, and the myosin-II tail...
September 15, 2017: ELife
https://www.readbyqxmd.com/read/28898058/mechanoregulation-of-sm22%C3%AE-transgelin
#2
Rong Liu, M Moazzem Hossain, Xuequn Chen, Jian-Ping Jin
SM22α, also named transgelin, is an actin filament-associated protein in smooth muscle and fibroblast. Three decades after discovery, the biological function of SM22α remains under investigation. Here we report a novel finding that the expression and degradation of SM22α/transgelin is regulated by mechanical tension. Following a mass spectrometry identification of SM22α degradation in isolated and tension-unloaded mouse aorta, we developed specific monoclonal antibodies to study the regulation of SM22α in human fetal lung myofibroblast line MRC-5 and primary cultures of neonatal mouse skin fibroblast...
September 12, 2017: Biochemistry
https://www.readbyqxmd.com/read/28889965/losartan-counteracts-the-effects-of-cardiomyocyte-swelling-on-glucose-uptake-and-insulin-receptor-substrate-1-levels
#3
Yamil Gerena, Janice Griselle Lozada, Bryan Jael Collazo, Jarold Méndez-Álvarez, Jennifer Méndez-Estrada, Walmor C De Mello
A growing body of evidence demonstrates an association between Angiotensin II (Ang II) receptor blockers (ARBs) and enhanced glucose metabolism during ischemic heart disease. Despite these encouraging results, the mechanisms responsible for these effects during ischemia remain poorly understood. In this study we investigated the influence of losartan, an AT1 receptor blocker, and secreted Ang II (sAng II) on glucose uptake and insulin receptor substrate (IRS-1) levels during cardiomyocyte swelling. H9c2 cells were differentiated to cardiac muscle and the levels of myogenin, Myosin Light Chain (MLC), and membrane AT1 receptors were measured using flow cytometry...
September 7, 2017: Peptides
https://www.readbyqxmd.com/read/28887414/progressive-polarity-loss-and-luminal-collapse-disrupt-tissue-organization-in-carcinoma
#4
Ruba Halaoui, Carlis Rejon, Sudipa June Chatterjee, Joseph Szymborski, Sarkis Meterissian, William J Muller, Atilla Omeroglu, Luke McCaffrey
Epithelial cancers (carcinoma) account for 80%-90% of all cancers. The development of carcinoma is associated with disrupted epithelial organization and solid ductal structures. The mechanisms underlying the morphological development of carcinoma are poorly understood, but it is thought that loss of cell polarity is an early event. Here we report the characterization of the development of human breast lesions leading to carcinoma. We identified a unique mechanism that generates solid ducts in carcinoma through progressive loss of polarity and collapse of the luminal architecture...
September 8, 2017: Genes & Development
https://www.readbyqxmd.com/read/28883443/planar-polarized-rab35-functions-as-an-oscillatory-ratchet-during-cell-intercalation-in-the-drosophila-epithelium
#5
Cayla E Jewett, Timothy E Vanderleest, Hui Miao, Yi Xie, Roopa Madhu, Dinah Loerke, J Todd Blankenship
The coordination between membrane trafficking and actomyosin networks is essential to the regulation of cell and tissue shape. Here, we examine Rab protein distributions during Drosophila epithelial tissue remodeling and show that Rab35 is dynamically planar polarized. Rab35 compartments are enriched at contractile interfaces of intercalating cells and provide the first evidence of interfacial monopolarity. When Rab35 function is disrupted, apical area oscillations still occur and contractile steps are observed...
September 7, 2017: Nature Communications
https://www.readbyqxmd.com/read/28870992/cell-migration-intercalation-and-growth-regulates-mammalian-cochlear-extension
#6
Elizabeth Carroll Driver, Amy Northrop, Matthew W Kelley
Developmental remodeling of the sensory epithelium of the cochlea is required for the formation of an elongated, tonotopically organized auditory organ, but the cellular processes that mediate these events are largely unknown. Therefore, we used both morphological assessments of cellular rearrangements and time-lapse imaging to visualize cochlear remodeling. Analysis of cell redistribution showed that the cochlea extends through a combination of radial intercalation and cell growth. Live imaging demonstrated that concomitant cellular intercalation results in a brief period of epithelial convergence, although subsequent changes in cell size lead to medial-lateral spreading...
September 4, 2017: Development
https://www.readbyqxmd.com/read/28867610/muscle-lim-protein-and-myosin-binding-protein-c-form-a-complex-regulating-muscle-differentiation
#7
Demetrios A Arvanitis, Elizabeth Vafiadaki, Vasiliki Papalouka, Despina Sanoudou
Muscle Lim Protein (MLP) is a protein with multiple functional roles in striated muscle physiology and pathophysiology. Herein, we demonstrate that MLP directly binds to slow, fast, and cardiac myosin-binding protein C (MyBP-C) during myogenesis, as shown by yeast two-hybrid, and a range of protein-protein interaction assays. The minimal interacting domains involve MLP inter-LIM and MyBP-C C4. The interaction is sensitive to cytosolic Ca(2+) concentrations changes and to MyBP-C phosphorylation by PKA or CaMKII...
August 31, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28865021/role-of-drebrin-in-synaptic-plasticity
#8
Yuko Sekino, Noriko Koganezawa, Toshiyuki Mizui, Tomoaki Shirao
Synaptic plasticity underlies higher brain function such as learning and memory, and the actin cytoskeleton in dendritic spines composing excitatory postsynaptic sites plays a pivotal role in synaptic plasticity. In this chapter, we review the role of drebrin in the regulation of the actin cytoskeleton during synaptic plasticity, under long-term potentiation (LTP) and long-term depression (LTD). Dendritic spines have two F-actin pools, drebrin-decorated stable F-actin (DF-actin) and drebrin-free dynamic F-actin (FF-actin)...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28865013/biochemistry-of-drebrin-and-its-binding-to-actin-filaments
#9
Ryoki Ishikawa
Drebrin is an actin-binding protein mainly expressed in developing neurons and dendritic spine in mature neurons. To understand the functions of drebrin in vivo, we must understand its molecular properties. In this chapter, I will focus on the purification and characterization of drebrin in vitro. Drebrin binds to F-actin with a stoichiometry of 1:5~6 with a K d of 1~3 × 10(-7) M and strongly inhibits the binding of other actin-binding proteins such as tropomyosin, caldesmon, fascin, α-actinin, and cofilin...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28842668/the-e-cadherin-amotl2-complex-organizes-actin-filaments-required-for-epithelial-hexagonal-packing-and-blastocyst-hatching
#10
Sebastian Hildebrand, Sara Hultin, Aravindh Subramani, Sophie Petropoulos, Yuanyuan Zhang, Xiaofang Cao, John Mpindi, Olli Kalloniemi, Staffan Johansson, Arindam Majumdar, Fredrik Lanner, Lars Holmgren
Epithelial cells connect via cell-cell junctions to form sheets of cells with separate cellular compartments. These cellular connections are essential for the generation of cellular forms and shapes consistent with organ function. Tissue modulation is dependent on the fine-tuning of mechanical forces that are transmitted in part through the actin connection to E-cadherin as well as other components in the adherens junctions. In this report we show that p100 amotL2 forms a complex with E-cadherin that associates with radial actin filaments connecting cells over multiple layers...
August 25, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28834398/er-golgi-trafficking-is-facilitated-by-unbranched-actin-filaments-containing-tpm4-2
#11
Anthony J Kee, Nicole S Bryce, Lingyan Yang, Elena Polishchuk, Galina Schevzov, Roberto Weigert, Roman Polishchuk, Peter W Gunning, Edna C Hardeman
We have identified novel actin filaments defined by tropomyosin Tpm4.2 at the ER. EM analysis of mouse embryo fibroblasts (MEFs) isolated from mice expressing a mutant Tpm4.2 (Tpm4(Plt53/Plt53) ), incapable of incorporating into actin filaments, revealed swollen ER structures compared to wild-type (WT) MEFs (Tpm4(+/+) ). ER-to-Golgi, but not Golgi-to-ER trafficking was altered in the Tpm4(Plt53/Plt53) MEFs following the transfection of the temperature sensitive ER-associated ts045-VSVg construct. Exogenous Tpm4...
August 22, 2017: Cytoskeleton
https://www.readbyqxmd.com/read/28829941/optimal-contractile-forces-for-a-mesenchymal-engine
#12
Dennis E Discher, Lawrence J Dooling
The cell and molecular mechanisms that cause a smooth epithelium to become topographically patterned during development are poorly understood. In a recent issue of Science, Shyer et al. (2017) show that myosin II contractility drives the smooth dermal mesenchyme into a pattern of surface bumps that triggers epithelial gene expression.
August 21, 2017: Developmental Cell
https://www.readbyqxmd.com/read/28825699/an-apical-mrck-driven-morphogenetic-pathway-controls-epithelial-polarity
#13
Ceniz Zihni, Evi Vlassaks, Stephen Terry, Jeremy Carlton, Thomas King Chor Leung, Michael Olson, Franck Pichaud, Maria Susana Balda, Karl Matter
Polarized epithelia develop distinct cell surface domains, with the apical membrane acquiring characteristic morphological features such as microvilli. Cell polarization is driven by polarity determinants including the evolutionarily conserved partitioning-defective (PAR) proteins that are separated into distinct cortical domains. PAR protein segregation is thought to be a consequence of asymmetric actomyosin contractions. The mechanism of activation of apically polarized actomyosin contractility is unknown...
September 2017: Nature Cell Biology
https://www.readbyqxmd.com/read/28821574/mosaic-loss-of-non-muscle-myosin-iia-and-iib-is-sufficient-to-induce-mammary-epithelial-proliferation
#14
Kim-Vy Nguyen-Ngoc, Vanesa L Silvestri, Dan Georgess, Amanda N Fairchild, Andrew J Ewald
The mammary epithelium elaborates through hormonally-regulated changes in proliferation, migration, and differentiation. Non-muscle myosin II (NMII) functions at the interface between contractility, adhesion, and signal transduction. It was therefore a plausible regulator of mammary morphogenesis. We tested the genetic requirement for NMIIA and NMIIB through deletion of the myosin heavy chains (NMHC) that confer specificity to the complex. Surprisingly, mosaic loss, but not ubiquitous loss, of NMHCIIA and IIB induced high levels of proliferation in 3D culture...
August 18, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28821556/dasatinib-reversibly-disrupts-endothelial-vascular-integrity-by-increasing-non-muscle-myosin-ii-contractility-in-a-rock-dependent-manner
#15
Anna Kreutzman, Beatriz Colom-Fernández, Ana Marcos Jiménez, Mette Ilander, Carlos Cuesta-Mateos, Yaiza Perez-García, Cristina Delgado Arévalo, Oscar Brück, Henna Hakanen, Jani Saarela, Alvaro Ortega-Carrión, Ana de Rosendo, Alba Juanes-García, Juan Luis Steegmann, Satu Mustjoki, Miguel Vicente-Manzanares, Cecilia Muñoz-Calleja
Purpose: Dasatinib is a short-acting dual ABL/SRC family tyrosine kinase inhibitor (TKI), which is frequently used to treat chronic myeloid leukemia. Although very effective, dasatinib often displays severe adverse effects, including pleural effusions and increased risk of bleeding primarily in the gastrointestinal tract. The actual causes of these side effects are currently undetermined. We hypothesize that endothelial cells (ECs) that line the inner walls of blood vessels and control the traffic to the underlying tissues, might be involved...
August 18, 2017: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/28820400/alteration-in-expressions-of-rhoa-and-rho-kinases-during-pregnancy-in-rats-their-roles-in-uterine-contractions-and-onset-of-labour
#16
D Domokos, E Ducza, G Falkay, R Gaspar
Activation of RhoA and Rho-associated kinases (ROCKs) is known to play a pivotal role in the regulation of smooth muscle contraction via phosphorylation of myosin-light chain and myosin phosphatase. There are few data on the RhoA and ROCKs expression levels in rat uteri. Therefore, our aim was to investigate the mRNA and protein concentration of RhoA and ROCKs in rat uterus during pregnancy, during parturition and post-partum using real time PCR and Western blot analysis. The other purpose was to evaluate the effects of the ROCK (Y-27632, fasudil and RKI 1441) and RhoA inhibitors (simvastatin) on uterine contractility in isolated organ bath experiments...
June 2017: Journal of Physiology and Pharmacology: An Official Journal of the Polish Physiological Society
https://www.readbyqxmd.com/read/28811312/myosin-ii-is-not-required-for-drosophila-tracheal-branch-elongation-and-cell-intercalation
#17
Amanda Ochoa-Espinosa, Stefan Harmansa, Emmanuel Caussinus, Markus Affolter
The Drosophila tracheal system consists of an interconnected network of monolayered epithelial tubes that ensures oxygen transport in the larval and adult body. During tracheal dorsal branch (DB) development, individual DBs elongate as a cluster of cells, led by tip cells at the front and trailing cells in the rear. Branch elongation is accompanied by extensive cell intercalation and cell lengthening of the trailing stalk cells. Although cell intercalation is governed by Myosin II (MyoII)-dependent forces during tissue elongation in the Drosophila embryo that lead to germ-band extension, it remained unclear whether MyoII plays a similar active role during tracheal branch elongation and intercalation...
August 15, 2017: Development
https://www.readbyqxmd.com/read/28810262/fetal-sheep-mesenteric-resistance-arteries-functional-and-structural-maturation
#18
Julia J Müller, Matthias Schwab, Charles R Rosenfeld, Iwa Antonow-Schlorke, Peter W Nathanielsz, Florian Rakers, Harald Schubert, Otto W Witte, Sven Rupprecht
BACKGROUND: Fetal blood pressure increases during late gestation; however, the underlying vascular mechanisms are unclear. Knowledge of the maturation of resistance arteries is important to identify the mechanisms and vulnerable periods for the development of vascular dysfunction in adulthood. METHODS: We determined the functional and structural development of fetal sheep mesenteric resistance arteries using wire myography and immunohistochemistry. RESULTS: Media mass and distribution of myosin heavy-chain isoforms showed no changes between 0...
August 16, 2017: Journal of Vascular Research
https://www.readbyqxmd.com/read/28808035/fission-yeast-myosin-myo2-is-down-regulated-in-actin-affinity-by-light-chain-phosphorylation
#19
Luther W Pollard, Carol S Bookwalter, Qing Tang, Elena B Krementsova, Kathleen M Trybus, Susan Lowey
Studies in fission yeast Schizosaccharomyces pombe have provided the basis for the most advanced models of the dynamics of the cytokinetic contractile ring. Myo2, a class-II myosin, is the major source of tension in the contractile ring, but how Myo2 is anchored and regulated to produce force is poorly understood. To enable more detailed biochemical/biophysical studies, Myo2 was expressed in the baculovirus/Sf9 insect cell system with its two native light chains, Rlc1 and Cdc4. Milligram yields of soluble, unphosphorylated Myo2 were obtained that exhibited high actin-activated ATPase activity and in vitro actin filament motility...
August 14, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28795988/eltrombopag-to-treat-thrombocytopenia-during-last-month-of-pregnancy-in-a-woman-with-myh9-related-disease-a-case-report
#20
Remi Favier, Celine De Carne, Elisabeth Elefant, Ruxanda Lapusneanu, Vasiliki Gkalea, Agnès Rigouzzo
MYH9-related disease (MYH9-RD) is an inherited rare autosomal dominant macrothrombocytopenia. Patients with MYH9-RD have giant platelets and leukocyte inclusion bodies caused by mutations in the MYH9 gene encoding the non-muscle myosin heavy chain II-A. Before identification of the causative gene, patients were diagnosed as Epstein or Fechtner or Sebastian syndromes or May-Hegglin anomaly. As with other inherited thrombocytopenias, the risk of increased bleeding during perioperative period or delivery is a major concern...
August 8, 2017: A & A Case Reports
keyword
keyword
106532
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"