keyword
MENU ▼
Read by QxMD icon Read
search

Collagen type I

keyword
https://www.readbyqxmd.com/read/29164999/collagen-type-i-a2-gene-polymorphisms-and-susceptibility-to-intracranial-aneurysms-a-meta-analysis-of-genetic-association-studies
#1
Alexandros G Brotis, Anastasia Tasiou, Theofanis Giannis, Athanasios Paschalis, Kostas N Fountas
BACKGROUND: The development, evolution and rupture of intracranial aneurysms is in part related to genetic factors. The role Collagen type I a2 genetic polymorphisms has not been clarified yet. MATERIAL AND METHODS: A meta-analysis was realized by means of a genotype model-fitting processes (allele contrast, recessive, dominant, additive, and co-dominant), and a model-free approach using the generalized odds ratio (ORG). The latter was assessed in association to the degree of dominance (h-index)...
November 22, 2017: International Journal of Neuroscience
https://www.readbyqxmd.com/read/29164001/paradoxical-elevation-of-serum-tracp5b-levels-despite-increase-in-lumbar-spine-bone-mineral-density-during-anti-tnf%C3%AE-therapy-in-patients-with-inflammatory-rheumatic-disease-a-2-year-prospective-assessment-of-bone-mass-bone-metabolism-and-the-trabecular-bone
#2
Éric Toussirot, Laurent Mourot, Barbara Dehecq, Fabrice Michel, Daniel Wendling, Émilie Grandclément, Gilles Dumoulin
Objective: To examine the impact of long-term anti-TNFα therapy on bone mass, bone metabolism, and the trabecular bone score (TBS) in patients with rheumatoid arthritis (RA) or ankylosing spondylitis (AS). Material and Methods: In eight patients with RA and 12 with AS, bone mineral densities (BMDs) of the lumbar spine (LS), left and right femoral neck, and total skeleton were measured using dual X-ray absorptiometry at baseline and then at 6, 12, and 24 months after anti TNFα therapy...
September 2017: European Journal of Rheumatology
https://www.readbyqxmd.com/read/29163675/effects-of-different-concentrations-of-type-i-collagen-hydrogel-on-the-growth-and-differentiation-of-chondrocytes
#3
Deshan Hu, Xiuli Shan
The objective of this study was to analyze the effects of type-I collagen hydrogel of different concentrations on the growth and differentiation of rabbit chondrocytes. Articular cartilage from New Zealand white rabbits was harvested and cultured. Second-generation chondrocytes were collected for in vitro culture with 10, 7, and 5 mg/ml type-I collagen hydrogel, respectively (denoted as groups A, B, and C). After in vitro culture for 1 day, chondrocytes were stained with fluorescein diacetate (FDA)/propidium iodide (PI), and cell viability was observed by laser confocal microscopy...
December 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29163221/the-superficial-dermis-may-initiate-keloid-formation-histological-analysis-of-the-keloid-dermis-at-different-depths
#4
Hu Jiao, Tiran Zhang, Jincai Fan, Ran Xiao
Several studies have reported on certain aspects of the characteristics of different sites within a keloid lesion, but detailed studies on the keloid dermis at different depths within a keloid lesion are scarce. The aim of this study was to investigate the histology of the keloid dermis at different depths. This study included 19 keloid tissue samples that were collected from 19 patients and 19 normal skin samples, which were harvested from subjects without keloids or hypertrophic scar. Samples were studied by light microscopy using routine hematoxylin and eosin histochemical staining, and immunohistochemistry to detect CD20-positive B-lymphocytes and CD3-positive T-lymphocytes...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/29162919/production-of-recombinant-human-procollagen-type-i-c-terminal-propeptide-and-establishment-of-a-sandwich-elisa-for-quantification
#5
Woo-Young Seo, Jeong-Ho Kim, Du-San Baek, Su-Jung Kim, Sujin Kang, Won Suk Yang, Ji-Ae Song, Moo-Seung Lee, Sunghoon Kim, Yong-Sung Kim
Procollagen type I carboxy-terminal propeptide (PICP), derived from type I procollagen, has been identified as an indicator of type I collagen synthesis in bone matrix formation and skin recovery. PICP is a heterotrimeric glycoprotein consisting of two α1 chains (PICPα1) and one α2 chain (PICPα2). Here, we report the recombinant expression of human PICP using a mammalian expression system. Co-expression of PICPα1 and PICPα2 in HEK293F cells resulted in the production of functional PICP in the correctly assembled heterotrimeric form...
November 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29162797/3d-collagen-architecture-induces-a-conserved-migratory-and-transcriptional-response-linked-to-vasculogenic-mimicry
#6
D O Velez, B Tsui, T Goshia, C L Chute, A Han, H Carter, S I Fraley
The topographical organization of collagen within the tumor microenvironment has been implicated in modulating cancer cell migration and independently predicts progression to metastasis. Here, we show that collagen matrices with small pores and short fibers, but not Matrigel, trigger a conserved transcriptional response and subsequent motility switch in cancer cells resulting in the formation of multicellular network structures. The response is not mediated by hypoxia, matrix stiffness, or bulk matrix density, but rather by matrix architecture-induced β1-integrin upregulation...
November 21, 2017: Nature Communications
https://www.readbyqxmd.com/read/29162108/reduced-suppressive-effect-of-%C3%AE-2-adrenoceptor-agonist-on-fibrocyte-function-in-severe-asthma
#7
Chun-Yu Lo, Charalambos Michaeloudes, Pankaj K Bhavsar, Chien-Da Huang, Po-Jui Chang, Chun-Hua Wang, Han-Pin Kuo, Kian Fan Chung
BACKGROUND: Patients with severe asthma have increased airway remodelling and elevated numbers of circulating fibrocytes with enhanced myofibroblastic differentiation capacity, despite being treated with high doses of corticosteroids, and long acting β2-adrenergic receptor (AR) agonists (LABAs). We determined the effect of β2-AR agonists, alone or in combination with corticosteroids, on fibrocyte function. METHODS: Non-adherent non-T cells from peripheral blood mononuclear cells isolated from healthy subjects and patients with non-severe or severe asthma were treated with the β2-AR agonist, salmeterol, in the presence or absence of the corticosteroid dexamethasone...
November 21, 2017: Respiratory Research
https://www.readbyqxmd.com/read/29161937/the-interplay-between-tgf-%C3%AE-smad-and-bmp-smad-signaling-pathways-in-the-epithelial-mesenchymal-transition-of-a549-cells-induced-by-silica
#8
Yanhua Yu, Yanming Lin, Gengxia Yang, Lin Tian
The epithelial-mesenchymal transition (EMT) is a phenotype transdifferentiation of epithelial into mesenchymal cells and contributes to pulmonary fibrotic disease. SMAD-dependent pathway has been reported to play a key role in the multiple fibrotic diseases. We hypothesized that TGF-β/SMAD signaling could cross-interact with BMP/SMAD signaling pathways in silica-induced EMT in A549 cells.We investigated that the ability of silica induced EMT in A549 cells, and this process was significantly inhibited by SB431542 through up-regulation of Vimentin, α-SMA and collagen type I expression and down-regulation of E-cadherin expression...
November 21, 2017: Toxicology Mechanisms and Methods
https://www.readbyqxmd.com/read/29161242/cadherin-1-and-cadherin-3-cooperation-determines-the-aggressiveness-of-pancreatic-ductal-adenocarcinoma
#9
Carole Siret, Aurélie Dobric, Anna Martirosyan, Chloé Terciolo, Sébastien Germain, Renaté Bonier, Thassadite Dirami, Nelson Dusetti, Richard Tomasini, Marion Rubis, Stéphane Garcia, Juan Iovanna, Dominique Lombardo, Véronique Rigot, Frédéric André
BACKGROUND: Pancreatic ductal adenocarcinoma (PDAC) is characterised by an extensive tissue invasion and an early formation of metastasis. Alterations in the expression of cadherins have been reported in PDAC. Yet, how these changes contribute to tumour progression is poorly understood. Here, we investigated the relationship between cadherins expression and PDAC development. METHODS: Cadherins expression was assessed by immunostaining in both human and murine tissue specimens...
November 21, 2017: British Journal of Cancer
https://www.readbyqxmd.com/read/29161138/autologous-matrix-induced-chondrogenesis-a-systematic-review-of-the-clinical-evidence
#10
Liang Gao, Patrick Orth, Magali Cucchiarini, Henning Madry
BACKGROUND: The addition of a type I/III collagen membrane in cartilage defects treated with microfracture has been advocated for cartilage repair, termed "autologous matrix-induced chondrogenesis" (AMIC). PURPOSE: To examine the current clinical evidence regarding AMIC for focal chondral defects. STUDY DESIGN: Systematic review. METHODS: A systematic review was performed by searching PubMed, ScienceDirect, and Cochrane Library databases...
November 1, 2017: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/29160747/lipopolysaccharide-mediates-hepatic-stellate-cell-activation-by-regulating-autophagy-and-retinoic-acid-signaling
#11
Ming Chen, Jiaxing Liu, Wenqi Yang, Wenhua Ling
Bacterial translocation and lipopolysaccharide (LPS) leakage occur at a very early stage of liver fibrosis in animal models. We studied the role of LPS in hepatic stellate cell (HSC) activation and the underlying mechanisms in vitro and in vivo. Herein, we demonstrated that LPS treatment led to a dramatic increase in autophagosome formation and autophagic flux in LX-2 cells and HSCs, which was mediated through the AKT-MTOR and AMPK-ULK1 pathway. LPS significantly decreased the lipid content, including the lipid droplet (LD) number and lipid staining area in HSCs; pretreatment with macroautophagy/autophagy inhibitors or silencing ATG5 attenuated this decrease...
November 21, 2017: Autophagy
https://www.readbyqxmd.com/read/29159895/proliferation-and-differentiation-of-mesenchymal-stem-cells-on-scaffolds-containing-chitosan-calcium-polyphosphate-and-pigeonite-for-bone-tissue-engineering
#12
S Dhivya, A Keshav Narayan, R Logith Kumar, S Viji Chandran, M Vairamani, N Selvamurugan
OBJECTIVES: Treatment of critical-sized bone defects with cells and biomaterials offers an efficient alternative to traditional bone grafts. Chitosan (CS) is a natural biopolymer that acts as a scaffold in bone tissue engineering (BTE). Polyphosphate (PolyP), recently identified as an inorganic polymer, acts as a potential bone morphogenetic material, whereas pigeonite (Pg) is a novel iron-containing ceramic. In this study, we prepared and characterized scaffolds containing CS, calcium polyphosphate (CaPP) and Pg particles for bone formation in vitro and in vivo...
November 21, 2017: Cell Proliferation
https://www.readbyqxmd.com/read/29159527/melatonin-ameliorates-intrarenal-renin-angiotensin-system-in-a-5-6-nephrectomy-rat-model
#13
Sayaka Ishigaki, Naro Ohashi, Takashi Matsuyama, Shinsuke Isobe, Naoko Tsuji, Takamasa Iwakura, Tomoyuki Fujikura, Takayuki Tsuji, Akihiko Kato, Hiroaki Miyajima, Hideo Yasuda
BACKGROUND: Activation of the intrarenal renin-angiotensin system (RAS) plays a critical role in the pathophysiology of chronic kidney disease (CKD) and hypertension. It has been reported that reactive oxygen species (ROS) are important components of intrarenal RAS activation. Melatonin is recognized as a powerful antioxidant, and we recently reported that impaired nighttime melatonin secretion correlates negatively with urinary angiotensinogen excretion, the surrogate marker of intrarenal RAS activity in patients with CKD...
November 20, 2017: Clinical and Experimental Nephrology
https://www.readbyqxmd.com/read/29158312/organotypic-three-dimensional-assays-based-on-human-leiomyoma-derived-matrices
#14
REVIEW
Tuula Salo, Mauricio Rocha Dourado, Elias Sundquist, Ehsanul Hoque Apu, Ilkka Alahuhta, Katja Tuomainen, Jenni Vasara, Ahmed Al-Samadi
Alongside cancer cells, tumours exhibit a complex stroma containing a repertoire of cells, matrix molecules and soluble factors that actively crosstalk between each other. Recognition of this multifaceted concept of the tumour microenvironment (TME) calls for authentic TME mimetics to study cancer in vitro Traditionally, tumourigenesis has been investigated in non-human, three-dimensional rat type I collagen containing organotypic discs or by means of mouse sarcoma-derived gel, such as Matrigel(®) However, the molecular compositions of these simplified assays do not properly simulate human TME...
January 5, 2018: Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences
https://www.readbyqxmd.com/read/29157905/a-new-collagenase-enzyme-of-the-marine-sponge-pathogen-pseudoalteromonas-agarivorans-nw4327-is-uniquely-linked-with-a-tonb-dependent-receptor
#15
Sayak Bhattacharya, Jayanta Debabrata Choudhury, Ratan Gachhui, Joydeep Mukherjee
The primary pathogen of the Great Barrier Reef sponge Rhopaloeides odorabile, recently identified as a novel strain (NW4327) of Pseudoalteromonas agarivorans, produced collagenase which degraded R. odorabile skeletal fibers. We now report the collagenase of P. agarivorans as a metalloprotease which required Ca(2+) and Zn(2+) as cofactors. The collagenase was a TonB dependent receptor (TBDR) having a carboxypeptidase regulatory like domain (CRLD) in the N-terminal along with an outer membrane(OM) channel superfamily domain...
November 17, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29156973/cartilage-defect-treatment-using-high-density-autologous-chondrocyte-implantation-two-year-follow-up
#16
Juan Manuel Lopez-Alcorocho, Lucia Aboli, Isabel Guillen-Vicente, Elena Rodriguez-Iñigo, Marta Guillen-Vicente, Tomás F Fernández-Jaén, Santiago Arauz, Steve Abelow, Pedro Guillen-García
OBJECTIVE: The aim of this work was to study the short- and mid-term effectiveness and safety of high-density autologous chondrocyte implantation (HD-ACI) in the first 50 patients with knee cartilage damage treated in our unit. DESIGN: Fifty consecutive patients with cartilage lesions (Outerbridge grade III-IV) in the knee treated with HD-ACI were included in this study. Chondrocytes were isolated from a nonbearing cartilage area biopsy and were cultured until 40 to 50 million cells were obtained...
January 1, 2017: Cartilage
https://www.readbyqxmd.com/read/29156943/assessment-of-biochemical-bone-turnover-markers-and-bone-mineral-density-in-thin-and-normal-weight-children
#17
Jadwiga Ambroszkiewicz, Joanna Gajewska, Grazyna Rowicka, Witold Klemarczyk, Magdalena Chelchowska
Objective There is scant research examining the prevalence of thinness in early childhood, despite its potential negative consequences for health and development across the life course. The objective of this study was to assess bone status through measurement of bone mineral density and biochemical bone turnover markers, with special attention paid to carboxylated (c-OC) as well as undercarboxylated (uc-OC) forms of osteocalcin, in the groups of thin and normal-weight children. Design The study included 80 healthy prepubertal children (median age 7...
January 1, 2017: Cartilage
https://www.readbyqxmd.com/read/29156148/cm-101-type-i-collagen-targeted-mr-imaging-probe-for-detection-of-liver-fibrosis
#18
Christian T Farrar, Eric M Gale, Richard Kennan, Ian Ramsay, Ricard Masia, Gunisha Arora, Kailyn Looby, Lan Wei, Jayashree Kalpathy-Cramer, Michelle M Bunzel, Chunlian Zhang, Yonghua Zhu, Taro E Akiyama, Michael Klimas, Shirly Pinto, Himashinie Diyabalanage, Kenneth K Tanabe, Valerie Humblet, Bryan C Fuchs, Peter Caravan
Purpose To evaluate the biodistribution, metabolism, and pharmacokinetics of a new type I collagen-targeted magnetic resonance (MR) probe, CM-101, and to assess its ability to help quantify liver fibrosis in animal models. Materials and Methods Biodistribution, pharmacokinetics, and stability of CM-101 in rats were measured with mass spectrometry. Bile duct-ligated (BDL) and sham-treated rats were imaged 19 days after the procedure by using a 1.5-T clinical MR imaging unit. Mice were treated with carbon tetrachloride (CCl4) or with vehicle two times a week for 10 weeks and were imaged with a 7...
November 20, 2017: Radiology
https://www.readbyqxmd.com/read/29155868/tocilizumab-potentially-prevents-bone-loss-in-patients-with-anticitrullinated-protein-antibody-positive-rheumatoid-arthritis
#19
Yi-Ming Chen, Hsin-Hua Chen, Wen-Nan Huang, Tsai-Ling Liao, Jun-Peng Chen, Wen-Cheng Chao, Ching-Tsai Lin, Wei-Ting Hung, Chia-Wei Hsieh, Tsu-Yi Hsieh, Yi-Hsing Chen, Der-Yuan Chen
Rheumatoid arthritis (RA) is associated with a high risk of osteoporosis and fracture. Interleukin (IL)-6 inhibitors may suppress osteoclast activation. Anticitrullinated protein antibody (ACPA) titers are inversely associated with bone mineral density (BMD). However, the differential effect of ACPA on bone turnover marker (BTM) and BMD changes after IL-6 inhibition remains unclear. This prospective study recruited patients with active RA with inadequate response to methotrexate or biologics. BMD was measured before and after 2-year tocilizumab (TCZ) treatment...
2017: PloS One
https://www.readbyqxmd.com/read/29152065/kahweol-decreases-hepatic-fibrosis-by-inhibiting-the-expression-of-connective-tissue-growth-factor-via-the-transforming-growth-factor-beta-signaling-pathway
#20
Hye-Young Seo, Yun-A Jung, So-Hee Lee, Jae Seok Hwang, Keun-Gyu Park, Mi-Kyung Kim, Byoung Kuk Jang
Kahweol is a diterpene molecule found in Coffea Arabica beans. Previous studies have shown that coffee reduces liver fibrosis, but it is not clear which component of coffee has the protective effect. In this study, we examined whether kahweol has a protective effect on hepatic fibrosis in vivo and in vitro. Kahweol decreased hepatic fibrosis by inhibiting connective tissue growth factor (CTGF) expression in thioacetamide (TAA)-treated mice. The expression of phospho-Smad3, signal transducer and activator of transcription 3 (STAT3), extracellular signal-regulated kinases (ERK), and c-Jun N-terminal protein kinase (JNK) increased in the livers of TAA-treated mice and decreased in the kahweol-treated group...
October 20, 2017: Oncotarget
keyword
keyword
106529
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"