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Collagen type I

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https://www.readbyqxmd.com/read/28109162/odontoblastic-differentiation-of-dental-pulp-stem-cells-from-healthy-and-carious-teeth-on-an-original-pcl-based-3d-scaffold
#1
A Louvrier, E Euvrard, L Nicod, G Rolin, F Gindraux, L Pazart, C Houdayer, P Y Risold, F Meyer, C Meyer
AIMS: To isolate and characterize Dental Pulp Stem Cells (DPSCs) obtained from carious and healthy mature teeth extracted when conservative treatment was not possible or for orthodontic reasons; to evaluate the ability of DPSCs to colonize, proliferate, and differentiate into functional odontoblast-like cells when cultured onto a polycaprolactone cone made by jet-spraying and prototyped into a design similar to a gutta-percha cone. METHODOLOGY: DPSCs were obtained from 9 carious and 12 healthy mature teeth...
January 21, 2017: International Endodontic Journal
https://www.readbyqxmd.com/read/28108956/post-implantation-fate-of-adipogenic-induced-mesenchymal-stem-cells-on-type-i-collagen-scaffold-in-a-rat-model
#2
Balu Venugopal, Francis B Fernandez, V S Harikrishnan, Annie John
Regenerative medicine via its application in soft tissue reconstruction through novel methods in adipose tissue engineering (ATE) has gained remarkable attention and investment despite simultaneous reports on clinical incidence of graft resorption and impaired vascularization. The underlying malaise here once identified may play a critical role in optimizing implant function. Our work attempts to determine the fate of donor cells and the implant in recipient micro environment using adipose-derived mesenchymal stem cells (ASCs) on a type I collagen sponge, an established scaffold for ATE...
February 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/28108777/a-randomized-controlled-trial-demonstrating-sustained-benefit-of-autologous-matrix-induced-chondrogenesis-over-microfracture-at-five%C3%A2-years
#3
Martin Volz, Jens Schaumburger, Hubert Frick, Joachim Grifka, Sven Anders
PURPOSE: Autologous Matrix-Induced Chondrogenesis (AMIC(®)) utilizing a type I/III collagen membrane was compared with microfracture (MFx) alone in focal cartilage lesions of the knee at one, two and five years. METHODS: Forty-seven patients (aged 37 ± 10 years, mean defect size 3.6 ± 1.6 cm(2)) were randomized and treated either with MFx, with sutured or glued AMIC(®) in a prospective multicentre clinical trial. RESULTS: After improvement for the first two years in all subgroups, a progressive and significant score degradation was observed in the MFx group, while all functional parameters remained stable for least five years in the AMIC(®) groups...
January 20, 2017: International Orthopaedics
https://www.readbyqxmd.com/read/28107614/sirt1-dependent-anti-senescence-effects-of-cell-deposited-matrix-on-human-umbilical-cord-mesenchymal-stem-cells
#4
Long Zhou, Xi Chen, Tao Liu, Caihong Zhu, Michelle Si, Joseph Jargstorf, Mao Li, Guoqing Pan, Yihong Gong, Zong-Ping Luo, Huilin Yang, Ming Pei, Fan He
Human umbilical cord-derived mesenchymal stem cells (UC-MSCs) are considered an attractive cell source for tissue regeneration. However, environmental oxidative stress can trigger premature senescence in MSCs and thus compromises their regenerative potential. Extracellular matrix (ECM) derived from MSCs has been shown to facilitate cell proliferation and multilineage differentiation. This investigation evaluated the effect of cell-deposited decellularized ECM (DECM) on oxidative stress-induced premature senescence in UC-MSCs...
January 20, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28107238/roles-of-the-endoplasmic-reticulum-stress-transducer-oasis-in-ossification-of-the-posterior-longitudinal-ligament
#5
Yu Chen, Haisong Yang, Jinhao Miao, Xiaowei Liu, Xinwei Wang, Deyu Chen
STUDY DESIGN: In vitro molecular research on the posterior longitudinal ligament fibroblasts. OBJECTIVE: To investigate different expression of old astrocyte specifically induced substance (OASIS) between spinal ligament fibroblasts from the patients with ossification of the posterior longitudinal ligament (OPLL) and from non-OPLL patients and demonstrate knockdown of OASIS protein expression by RNA interference inhibiting expression of type I collagen (COL I) in OPLL cells...
February 2017: Clinical Spine Surgery
https://www.readbyqxmd.com/read/28106724/osteocalcin-mediates-biomineralization-during-osteogenic-maturation-in-human-mesenchymal-stromal-cells
#6
Yu-Tzu Tsao, Yi-Jeng Huang, Hao-Hsiang Wu, Yu-An Liu, Yi-Shiuan Liu, Oscar K Lee
There is a growing interest in cell therapies using mesenchymal stromal cells (MSCs) for repairing bone defects. MSCs have the ability to differentiate into osteoprogenitors and osteoblasts as well as to form calcified bone matrix. However, the molecular mechanisms governing mineralization during osteogenic differentiation remain unclear. Non-collagenous proteins in the extracellular matrix are believed to control different aspects of the mineralization. Since osteocalcin is the most abundant non-collagenous bone matrix protein, the purpose of this study is to investigate the roles of osteocalcin in mineral species production during osteogenesis of MSCs...
January 17, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28105983/effect-of-bone-marrow-aspirate-concentrate-platelet-rich-plasma-on-tendon-derived-stem-cells-and-rotator-cuff-tendon-tear
#7
Sun Jeong, Da Hyun, Jong Wook, Silvia Park, Sang Jun
Bone marrow aspirate concentrates (BMACs) and platelet-rich plasma (PRP) are good sources to control the differentiation of tendon derived stem cells (TDSCs) but there has been no study about the effect of BMAC-PRP complex on TDSCs and tendinopathy. The aim of this study was to investigate the effect of BMAC-PRP on the TDSCs and to find the therapeutic effect of BMAC-PRP on the rotator cuff tendon tear. Chondrogenic and osteogenic potential of TDSCs decreased, but adipogenic potential of TDSCs revealed no significant difference when they were co-cultured with BMAC-PRP...
January 20, 2017: Cell Transplantation
https://www.readbyqxmd.com/read/28105216/identification-of-potential-therapeutic-targets-for-colorectal-cancer-by-bioinformatics-analysis
#8
Ming Yan, Maomin Song, Rixing Bai, Shi Cheng, Wenmao Yan
The aim of the present study was to identify potential therapeutic targets for colorectal cancer (CRC). The gene expression profile GSE32323, containing 34 samples, including 17 specimens of CRC tissues and 17 of paired normal tissues from CRC patients, was downloaded from the Gene Expression Omnibus database. Following data preprocessing using the Affy and preprocessCore packages, the differentially-expressed genes (DEGs) between the two types of samples were identified with the Linear Models for Microarray Analysis package...
December 2016: Oncology Letters
https://www.readbyqxmd.com/read/28105116/computer-assisted-quantitative-evaluation-of-bisphosphonate-treatment-for-paget-s-disease-of-bone-using-the-bone-scan-index
#9
Satoshi Nagano, Shunsuke Nakamura, Hirofumi Shimada, Masahiro Yokouchi, Takao Setoguchi, Yasuhiro Ishidou, Hiromi Sasaki, Setsuro Komiya
The purpose of the present study was to analyze the effect of treatment of Paget's disease of bone (PDB) with bone scintigraphy using a computer-assisted diagnosis system (BONENAVI) that quantitatively evaluates bone metabolism. Seven patients with PDB (three male, four female; average age, 60 years; age range, 33-80 years) underwent bone scintigraphy and measurement of serum alkaline phosphatase (ALP), bone-specific ALP (BAP), serum cross-linked N-telopeptide (NTx) of type I collagen, urinary NTx, and deoxypyridinoline (DPD) before and after bisphosphonate treatment...
December 2016: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28105092/serum-n-terminal-telopeptide-of-type-i-collagen-as-an-early-marker-of-fracture-nonunion-in-rabbits
#10
Jian-Ping Lin, Zhan-Jun Shi, Ning-Jiang Shen, Jian Wang, Zao-Min Li, Jun Xiao
The aim of the present study was to establish an experimental animal model of fracture nonunion, and to investigate the changes in serum biomarker concentrations in fracture nonunion. A total of 20 purebred New Zealand rabbits were divided into two group: A bone defect group and a bone fracture group. In the bone defect group, a 15-mm section of bone (including the periosteum) was removed from the mid-radius, and the medullary cavities were closed with bone wax. In the bone fracture group, the mid-radius was fractured...
December 2016: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28104572/progressive-heart-disease-in-mucopolysaccharidosis-type-i-mice-may-be-mediated-by-increased-cathepsin-b-activity
#11
Guilherme Baldo, Angela Maria Vicente Tavares, Esteban Gonzalez, Edina Poletto, Fabiana Quoos Mayer, Ursula da Silveira Matte, Roberto Giugliani
Mucopolysaccharidosis type I (MPS I) is a lysosomal disorder characterized by a deficiency of alpha-L-iduronidase and storage of undegraded glycosaminoglycans (GAGs). Clinical findings of the disease include heart failure, and patients often need valve replacement. It has been shown that, later in life, MPS I mice develop those abnormalities, but to date, there have not been studies on the progression and pathogenesis of the disease. Therefore, in the present study, we evaluated heart function in normal and MPS I male mice from 2 to 8 months of age...
January 6, 2017: Cardiovascular Pathology: the Official Journal of the Society for Cardiovascular Pathology
https://www.readbyqxmd.com/read/28103936/elevated-collagen-i-augments-tumor-progressive-signals-intravasation-and-metastasis-of-prolactin-induced-estrogen-receptor-alpha-positive-mammary-tumor-cells
#12
Craig E Barcus, Kathleen A O'Leary, Jennifer L Brockman, Debra E Rugowski, Yuming Liu, Nancy Garcia, Menggang Yu, Patricia J Keely, Kevin W Eliceiri, Linda A Schuler
BACKGROUND: The development and progression of estrogen receptor alpha positive (ERα+) breast cancer has been linked epidemiologically to prolactin. However, activation of the canonical mediator of prolactin, STAT5, is associated with more differentiated cancers and better prognoses. We have reported that density/stiffness of the extracellular matrix potently modulates the repertoire of prolactin signals in human ERα + breast cancer cells in vitro: stiff matrices shift the balance from the Janus kinase (JAK)2/STAT5 cascade toward pro-tumor progressive extracellular regulated kinase (ERK)1/2 signals, driving invasion...
January 19, 2017: Breast Cancer Research: BCR
https://www.readbyqxmd.com/read/28103932/biomarkers-of-extracellular-matrix-turnover-are-associated-with-emphysema-and-eosinophilic-bronchitis-in-copd
#13
Asger Reinstrup Bihlet, Morten Asser Karsdal, Jannie Marie Bülow Sand, Diana Julie Leeming, Mustimbo Roberts, Wendy White, Russell Bowler
BACKGROUND: Chronic obstructive pulmonary disease (COPD) is characterized by airflow obstruction and loss of lung tissue mainly consisting of extracellular matrix (ECM). Three of the main ECM components are type I collagen, the main constituent in the interstitial matrix, type VI collagen, and elastin, the signature protein of the lungs. During pathological remodeling driven by inflammatory cells and proteases, fragments of these proteins are released into the bloodstream, where they may serve as biomarkers for disease phenotypes...
January 19, 2017: Respiratory Research
https://www.readbyqxmd.com/read/28103327/isolation-characterization-and-evaluation-of-collagen-from-jellyfish-rhopilema-esculentum-kishinouye-for-use-in-hemostatic-applications
#14
Xiaochen Cheng, Ziyu Shao, Chengbo Li, Lejun Yu, Mazhar Ali Raja, Chenguang Liu
Hemostat has been a crucial focus since human body is unable to control massive blood loss, and collagen proves to be an effective hemostat in previous studies. In this study, collagen was isolated from the mesoglea of jellyfish Rhopilema esculentum Kishinouye and its hemostatic property was studied. The yields of acid-soluble collagen (ASC) and pepsin-soluble (PSC) were 0.12% and 0.28% respectively. The SDS-PAGE patterns indicated that the collagen extracted from jellyfish mesoglea was type I collagen. The lyophilized jellyfish collagen sponges were cross-linked with EDC and interconnected networks in the sponges were revealed by scanning electron microscope (SEM)...
2017: PloS One
https://www.readbyqxmd.com/read/28102826/ceramic-particle-engulfment-by-fibroblasts-does-not-alter-at-all-cell-behavior
#15
Pierre-Antoine Faye, Olivier Roualdes, Fabrice Rossignol, Daniel Jean Hartmann, Alexis Desmoulière
Despite many studies, the impact of ceramic particles on cell behavior remains still unclear. The aim of the present study was to investigate the effects of ceramic nano-sized particles on fibroblastic cells. Fibroblasts (dermal fibroblasts freshly isolated from skin samples and WI26 fibroblastic cells) were cultured in monolayer in the presence of alumina or cerium-zirconia particles (��� 50 nm diameter) at two concentrations (100 or 500 ��g.mL-1). Fluorescent alumina particles were also used. Cell morphology, cytoplasmic ceramic incorporation using confocal and transmission electron microscopy, and migration using silicon insert, were analyzed...
January 19, 2017: Biomedical Materials
https://www.readbyqxmd.com/read/28102596/delineation-of-ehlers-danlos-syndrome-phenotype-due-to-the-c-934c-t-p-arg312cys-mutation-in-col1a1-report-on-a-three-generation-family-without-cardiovascular-events-and-literature-review
#16
Marina Colombi, Chiara Dordoni, Marina Venturini, Arianna Zanca, Piergiacomo Calzavara-Pinton, Marco Ritelli
Classical Ehlers-Danlos syndrome (cEDS) is a rare connective tissue disorder primarily characterized by hyperextensible skin, defective wound healing, abnormal scars, easy bruising, and generalized joint hypermobility; arterial dissections are rarely observed. Mutations in COL5A1 and COL5A2 encoding type V collagen account for more than 90% of the patients so far characterized. In addition, cEDS phenotype was reported in a small number of patients carrying the c.934C>T mutation in COL1A1 that results in an uncommon substitution of a non-glycine residue in one Gly-Xaa-Yaa repeat of the pro-α1(I)-chain p...
February 2017: American Journal of Medical Genetics. Part A
https://www.readbyqxmd.com/read/28101909/efficient-transdifferentiation-of-human-dermal-fibroblasts-into-skeletal-muscle
#17
Selwa Mokhtar Boularaoui, Khaled M A Abdel-Raouf, Noaf Salah Ali Alwahab, Megan E Kondash, George A Truskey, Jeremy Choon Meng Teo, Nicolas Christoforou
Skeletal muscle holds significant regenerative potential but is incapable of restoring tissue loss caused due to severe injury, congenital defects, or tumor ablation. Consequently, skeletal muscle models are being developed to study human pathophysiology and regeneration. Their physiological accuracy, however, is hampered by the lack of an easily accessible human cell source that is readily expandable and capable of efficient differentiation. MYOD1, a master gene regulator, induces transdifferentiation of a variety of cell types into skeletal muscle, although inefficiently in human cells...
January 18, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28101870/fibrillar-collagens
#18
Jordi Bella, David J S Hulmes
Fibrillar collagens (types I, II, III, V, XI, XXIV and XXVII) constitute a sub-group within the collagen family (of which there are 28 types in humans) whose functions are to provide three-dimensional frameworks for tissues and organs. These networks confer mechanical strength as well as signalling and organizing functions through binding to cellular receptors and other components of the extracellular matrix (ECM). Here we describe the structure and assembly of fibrillar collagens, and their procollagen precursors, from the molecular to the tissue level...
2017: Sub-cellular Biochemistry
https://www.readbyqxmd.com/read/28101149/effects-of-nano-tantalum-implants-on-inducing-osteoblast-proliferation-and-differentiation
#19
Xinyu Liu, Xiaobin Song, Peng Zhang, Zhenkun Zhu, Xin Xu
In the present study, we examined the effects of nano tantalum (Ta) dental implants on inducing osteoblast proliferation and differentiation. The MG-63 osteoblasts were divided into 3 groups after recovery, passage and storage: i) Osteoblast culturing group (control group); ii) osteoblast and titanium (Ti) implant co-culturing group (Ti group); and iii) osteoblast and Ta implant co-culturing group (Ta group). After 7 days, a scanning electron microscope was used to observe the growth status, number and morphological changes of the cells on the surfaces of the materials...
December 2016: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28101144/histopathological-findings-in-systemic-sclerosis-related-myopathy-fibrosis-and-microangiopathy-with-lack-of-cellular-inflammation
#20
Claudio Corallo, Maurizio Cutolo, Nila Volpi, Daniela Franci, Margherita Aglianò, Antonio Montella, Chiara Chirico, Stefano Gonnelli, Ranuccio Nuti, Nicola Giordano
OBJECTIVES: The objective of this study was to identify specific histopathological features of skeletal muscle involvement in systemic sclerosis (SSc) patients. METHODS: A total of 35 out of 112 SSc-patients (32%, including 81% female and 68% diffuse scleroderma) presenting clinical, biological and electromyographic (EMG) features of muscle weakness, were included. Patients underwent vastus lateralis biopsy, assessed for individual pathologic features including fibrosis [type I collagen (Coll-I), transforming growth factor β (TGF-β)], microangiopathy [cluster of differentiation 31 (CD31), pro-angiogenic vascular endothelial growth factor A (VEGF-A), anti-angiogenic VEGF-A165b], immune/ inflammatory response [CD4, CD8, CD20, human leucocyte antigens ABC (HLA-ABC)], and membranolytic attack complex (MAC)...
January 2017: Therapeutic Advances in Musculoskeletal Disease
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