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Self-assembling peptide

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https://www.readbyqxmd.com/read/28820922/a-self-assembling-peptide-gel-as-a-vitreous-substitute-a-rabbit-study
#1
Koji Uesugi, Hirokazu Sakaguchi, Yasutaka Hayashida, Ryuhei Hayashi, Koichi Baba, Yuya Suganuma, Hidenori Yokoi, Motokazu Tsujikawa, Kohji Nishida
Purpose: To evaluate a self-assembling peptide gel as a potential vitreous substitute. Methods: PanaceaGel SPG-178, a self-assembling peptide gel, was diluted with distilled water and a balanced salt solution to achieve a final peptide concentration of 0.1%. The gel's refractive index, visible light transmission rate, and rheologic properties were investigated. The gel's biocompatibility was evaluated by examining the cellular viability (live and dead staining) and proliferation rate (alamarBlue assay)...
August 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28818724/enzyme-sensitive-smart-inulin-dehydropeptide-conjugate-self-assembles-into-nanostructures-useful-for-targeted-delivery-of-ornidazole
#2
Kriti Shivhare, Charu Garg, Ayushi Priyam, Alka Gupta, Ashwani Kumar Sharma, Pradeep Kumar
Molecular self-assembly of biodegradable amphiphilic polymers allows rational design of biocompatible nanomaterials for drug delivery. Use of substituted polysaccharides for such applications offers the ease of design and synthesis, and provides higher biofunctionality and biocompatibility to nanomaterials. The present work focuses on the synthesis, characterization and potential biomedical applications of self-assembled polysaccharide-based materials. We demonstrated that the synthesized amphiphilic inulin self-assembled in aqueous medium into nanostructures with average size in the range of 146-486nm and encapsulated hydrophobic therapeutic molecule, ornidazole...
August 14, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28817259/sub-20-nm-stable-micelles-based-on-a-mixture-of-coiled-coils-a-platform-for-controlled-ligand-presentation
#3
JooChuan Ang, Dan Ma, Benson Thomas Jung, Sinan Keten, Ting Xu
Ligand-functionalized, multivalent nanoparticles have been extensively studied for biomedical applications from imaging agents to drug delivery vehicles. However, the ligand cluster size is usually heterogeneous and the local valency is ill-defined. Here, we present a mixed micelle platform hierarchically self-assembled from a mixture of two amphiphilic 3-helix and 4-helix peptide-PEG-lipid hybrid conjugates. We demonstrate that the local multivalent ligand cluster size on the micelle surface can be controlled based on the coiled-coil oligomeric state...
August 17, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28817256/tailorable-exciton-transport-in-doped-peptide-amphiphile-assemblies
#4
Lee A Solomon, Matthew E Sykes, Yimin A Wu, Richard D Schaller, Gary P Wiederrecht, Harry Christopher Fry
Light-harvesting biomaterials are an attractive target in photovoltaics, photocatalysis, and artificial photosynthesis. Through peptide self-assembly, complex nanostructures can be engineered to study the role of chromophore organization during light absorption and energy transport. To this end, we demonstrate the one-dimensional transport of excitons along naturally occurring, light-harvesting, Zn-protoporphyrin IX chromophores within self-assembled peptide-amphiphile nanofibers. The internal structure of the nanofibers induces packing of the porphyrins into linear chains...
August 17, 2017: ACS Nano
https://www.readbyqxmd.com/read/28816368/one-pot-self-assembly-of-peptide-based-cage-type-nanostructures-using-orthogonal-ligations
#5
Eline Bartolami, Jérémie Knoops, Yannick Bessin, Mathieu Fossépré, Joseph Chamieh, Pascal Dumy, Mathieu Surin, Sebastien Ulrich
The designed arrangement of biomolecular entities within monodisperse nanostructures is an important challenge toward functional biomaterials. We report herein a method for the formation of water-soluble peptide-based cages using orthogonal ligation reactions - acylhydrazone condensation and thiol-maleimide addition. The results show that using pre-organized cyclic peptides and heterobifunctional spacers as building blocks and a set of orthogonal and chemoselective ligation reactions enable cage formation in one-pot from six components and through eight reactions...
August 17, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28816007/fluorous-phase-directed-peptide-assembly-affords-nano-peptisomes-capable-of-ultrasound-triggered-cellular-delivery
#6
Scott H Medina, Megan S Michie, Stephen E Miller, Martin J Schnermann, Joel P Schneider
Here, we report the design, synthesis and efficacy of a new class of ultrasound (US)-sensitive self-assembled peptide-based nanoparticle. Peptisomes are prepared via templated assembly of a de novo designed peptide at the interface of fluorinated nanodroplets. Utilizing peptide assembly allows for facile particle synthesis, direct incorporation of bioactive sequences displayed from the particle corona, and the ability to easily encapsulate biologics during particle preparation using a mild solvent exchange procedure...
August 16, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28812982/quantitative-criteria-to-benchmark-new-and-existing-bio-inks-for-cell-compatibility
#7
Karen Dubbin, Anthony Tabet, Sarah Heilshorn
Recent advancements in 3D bioprinting have led to the fabrication of more complex, more precise, and larger printed tissue constructs. As the field continues to advance, it is critical to develop quantitative benchmarks to compare different bio-inks for key cell-biomaterial interactions, including (1) cell sedimentation within the ink cartridge, (2) cell viability during extrusion, and (3) cell viability after ink curing. Here we develop three simple protocols for quantitative analysis of bio-ink performance...
August 16, 2017: Biofabrication
https://www.readbyqxmd.com/read/28812358/bioinspired-active-stealth-magneto-nanomicelles-for-theranostics-combining-efficient-mri-and-enhanced-drug-delivery
#8
Kai-Long Zhang, Jie Zhou, Hong Zhou, Ying Wu, Rui Liu, Li-Li Wang, Wei-Wen Lin, Guoming Huang, Huang-Hao Yang
The mononuclear phagocyte system (MPS), with key roles in recognition and clearance of foreign particles, is a major constraint to nanoparticle-based delivery systems. The desire to improve the delivery efficiency has prompted the search for stealthy long-circulating nanoplatforms. Herein, we design an antiphagocytic delivery system with "active" stealth behavior for cancer theranostics combining efficient MRI and enhanced drug delivery. We modify self-peptide, a synthetic peptide for active immunomodulation, to biodegradable poly (lactide-glycolide)-poly (ethylene glycol) (PLGA-PEG), then utilize the self-assembly properties of PLGA-PEG to form nanomicelles that encapsulating iron oxide (IO) nanoparticles and anticancer drug paclitaxel (PTX)...
August 16, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28812314/self-assembled-chiral-nanostructures-of-amphiphilic-peptide-from-single-molecule-to-aggregate
#9
Ting Zhou, Zhiqing Zhang, Xuemei Zhang, Chen Wang, Guiying Xu, Yanlian Yang
We report interesting hierarchical self-assembled architectures from a designed amphiphilic peptide. The bisignate cotton effects in circular dichroism spectra show typical peptide aggregation-induced. The observation of peptide assembly structures from initial particles and fibrils to ribbon structures is supported by microscopy (atomic force microscopy and transmission electron microscopy). The visualization of individual peptide at the single molecular level offered insights of the intermolecular interactions responsible for the formation of aggregates, which is investigated by scanning tunneling microscopy...
August 15, 2017: Journal of Peptide Science: An Official Publication of the European Peptide Society
https://www.readbyqxmd.com/read/28808707/molecular-co-assembly-as-a-strategy-for-synergistic-improvement-of-the-mechanical-properties-of-hydrogels
#10
Michal Halperin-Sternfeld, Moumita Ghosh, Rina Sevostianov, Irena Grigoriants, Lihi Adler-Abramovich
Molecular self-assembly is a key direction for the fabrication of advanced materials. Yet, the physical properties of the formed assemblies are limited by the inherent characteristics of the specific building blocks. Here, we have applied a co-assembly approach to synergistically modulate the mechanical properties of peptide hydrogels, thereby forming extremely stable and rigid hydrogels.
August 15, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28807783/aggregation-and-its-influence-on-the-immunomodulatory-activity-of-synthetic-innate-defense-regulator-peptides
#11
Evan F Haney, Bing Catherine Wu, Kelsey Lee, Ashley L Hilchie, Robert E W Hancock
There is increasing interest in developing cationic host defense peptides (HDPs) and their synthetic derivatives as antimicrobial, immunomodulatory, and anti-biofilm agents. These activities are often evaluated without considering biologically relevant concentrations of salts or serum; furthermore certain HDPs have been shown to aggregate in vitro. Here we examined the effect of aggregation on the immunomodulatory activity of a synthetic innate defense regulator peptide, 1018 (VRLIVAVRIWRR-NH2). A variety of salts and solutes were screened to determine their influence on 1018 aggregation, revealing that this peptide "salts out" of solution in an anion-specific and concentration-dependent manner...
July 24, 2017: Cell Chemical Biology
https://www.readbyqxmd.com/read/28803479/design-of-cyclic-peptides-based-glucose-receptors-and-their-application-in-glucose-sensing
#12
Chao Li, Xin Chen, Fuyuan Zhang, Xingxing He, Guozhen Fang, Jifeng Liu, Shuo Wang
Glucose assay is of great scientific significance in clinical diagnostics and bioprocess monitoring and to design new glucose receptor is necessary for development of more sensitive, selective and robust glucose detection techniques. Herein, a series of cyclic peptides (CPs) glucose receptor were designed to mimic the binding sites of glucose binding protein (GBP) and CPs' sequence contained amino acid sites Asp, Asn, His, Asp, Arg, which constituted the first layer interactions of GBP. The properties of these CPs used as a glucose receptor or substitute for the GBP were studied by using quartz crystal microbalance (QCM) technique...
August 14, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28799716/rational-design-of-membrane-pore-forming-peptides
#13
Max Pillong, Jan A Hiss, Petra Schneider, Yen-Chu Lin, Gernot Posselt, Bernhard Pfeiffer, Markus Blatter, Alex T Müller, Simon Bachler, Claudia S Neuhaus, Petra S Dittrich, Karl-Heinz Altmann, Silja Wessler, Gisbert Schneider
Specific interactions of peptides with lipid membranes are essential for cellular communication and constitute a central aspect of the innate host defense against pathogens. A computational method for generating innovative membrane-pore-forming peptides inspired by natural templates is presented. Peptide representation in terms of sequence- and topology-dependent hydrophobic moments is introduced. This design concept proves to be appropriate for the de novo generation of first-in-class membrane-active peptides with the anticipated mode of action...
August 11, 2017: Small
https://www.readbyqxmd.com/read/28799590/potentiating-the-immune-response-of-muc1-based-antitumor-vaccines-using-a-peptide-based-nanovector-as-a-promising-vaccine-adjuvant
#14
Yonghui Liu, Youzhi Wang, Fan Yu, Zhenqing Zhang, Zhimou Yang, Wenpeng Zhang, Peng George Wang, Wei Zhao
We utilize the supramolecular self-assembling peptide of Nap-G(D)F(D)F(D)Y(D)K to construct chemically programmed, self-assembling and self-adjuvant MUC1-based antitumor vaccines. The vaccines, with antigen and adjuvant conjugation through covalent bonds, elicited both humoral and cellular immune responses.
August 11, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28793213/critical-influence-of-cosolutes-and-surfaces-on-the-assembly-of-serpin-derived-amyloid-fibrils
#15
Michael W Risør, Dennis W Juhl, Morten Bjerring, Joachim Mathiesen, Jan J Enghild, Niels C Nielsen, Daniel E Otzen
Many proteins and peptides self-associate into highly ordered and structurally similar amyloid cross-β aggregates. This fibrillation is critically dependent on properties of the protein and the surrounding environment that alter kinetic and thermodynamic equilibria. Here, we report on dominating surface and solution effects on the fibrillogenic behavior and amyloid assembly of the C-36 peptide, a circulating bioactive peptide from the α1-antitrypsin serine protease inhibitor. C-36 converts from an unstructured peptide to mature amyloid twisted-ribbon fibrils over a few hours when incubated on polystyrene plates under physiological conditions through a pathway dominated by surface-enhanced nucleation...
August 8, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28792044/enzyme-assisted-peptide-folding-assembly-and-anti-cancer-properties
#16
Chunhui Liang, Debin Zheng, Fang Shi, Tengyan Xu, Cuihong Yang, Jianfeng Liu, Ling Wang, Zhimou Yang
The α-helix is the most prevalent conformation in proteins. However, formation of the α-helical conformation remains a challenge for short peptides with less than 5 amino acids. We demonstrated in this study that enzyme-instructed self-assembly (EISA) provides a unique pathway to assist the self-assembly of peptides into the α-helical conformation, while a heating-cooling process leads to a conformation more similar to a β-sheet. The same peptide with different conformations self-assembled into different nanostructures...
August 9, 2017: Nanoscale
https://www.readbyqxmd.com/read/28791965/design-of-bfgf-tethered-self-assembling-extracellular-matrix-proteins-via-coiled-coil-triple-helix-formation
#17
Yoshinori Mizuguchi, Yasumasa Mashimo, Masayasu Mie, Eiry Kobatake
Self-assembling peptides are attractive materials for tissue engineering applications because of their functionality including high biocompatibility and biodegradability. Modification of self-assembling peptides with functional motifs, such as the cell-adhesive tripeptide sequence RGD leads to functional artificial extracellular matrices (ECMs). In this study, we developed an artificial self-assembling ECM protein tethered with a growth factor via heterotrimer triple-helix (helix A/B/C) formation. The helix A and helix C peptides, which are capable of forming a heterodimer coiled-coil structure, were fused to both ends of a matrix protein composed of the elastin-derived structural unit (APGVGV)12 with an RGD motif...
August 9, 2017: Biomedical Materials
https://www.readbyqxmd.com/read/28790398/shedding-light-on-alzheimer-s-%C3%AE-amyloidosis-photosensitized-methylene-blue-inhibits-self-assembly-of-%C3%AE-amyloid-peptides-and-disintegrates-their-aggregates
#18
Byung Il Lee, Yoon Seok Suh, You Jung Chung, Kweon Yu, Chan Beum Park
Abnormal aggregation of β-amyloid (Aβ) peptides is a major hallmark of Alzheimer's disease (AD). In spite of numerous attempts to prevent the β-amyloidosis, no effective drugs for treating AD have been developed to date. Among many candidate chemicals, methylene blue (MB) has proved its therapeutic potential for AD in a number of in vitro and in vivo studies; but the result of recent clinical trials performed with MB and its derivative was negative. Here, with the aid of multiple photochemical analyses, we first report that photoexcited MB molecules can block Aβ42 aggregation in vitro...
August 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28782928/biodegradable-micelles-based-on-poly-ethylene-glycol-b-polylipopeptide-copolymer-a-robust-and-versatile-nanoplatform-for-anticancer-drug-delivery
#19
Min Qiu, Jia Ouyang, Huanli Sun, Fenghua Meng, Ru Cheng, Jian Zhang, Liang Cheng, Qing Lan, Chao Deng, Zhiyuan Zhong
Poly(ethylene glycol)-b-polypeptide block copolymer micelles with excellent safety are one of the most clinically studied nanocarriers for anticancer drug delivery. Notably, self-assembled nanosystems based on hydrophobic polypeptides showing typically a low drug loading and burst drug release are limited to preclinical studies. Here, we report that poly(ethylene glycol)-b-poly(α-aminopalmitic acid) (PEG-b-PAPA) block copolymer could be easily prepared with tailored Mn through ring-opening polymerization of α-aminopalmitic acid N-carboxyanhydride (APA-NCA)...
August 7, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28782856/an-on-site-transformation-strategy-for-treatment-of-bacterial-infection
#20
Guo-Bin Qi, Di Zhang, Fu-Hua Liu, Zeng-Ying Qiao, Hao Wang
To date, numerous nanosystems have been developed as antibiotic replacements for bacterial infection treatment. However, these advanced systems are limited owing to their nontargeting accumulation and the consequent side effects. Herein, transformable polymer-peptide biomaterials have been developed that enable specific accumulation in the infectious site and long-term retention, resulting in enhanced binding capability and killing efficacy toward bacteria. The polymer-peptide conjugates are composed of a chitosan backbone and two functional peptides, i...
August 7, 2017: Advanced Materials
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