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https://www.readbyqxmd.com/read/28422167/biotin-transfer-from-a-trifunctional-crosslinker-for-identification-of-cell-surface-receptors-of-soluble-protein-ligands
#1
Tammy-Lynn Tremblay, Jennifer J Hill
Here we describe a novel crosslinker and its application as a biotin-transfer reagent to identify cell surface receptors of soluble protein ligands on live cells. This crosslinker contains three functional groups: an aldehyde-reactive aminooxy group, a sulfhydryl, and a biotin (ASB). It is readily synthesized via a 3-step addition reaction using standard solid-phase peptide synthesis methods and commercially available intermediates, allowing access to laboratories without specialized synthetic chemistry capabilities...
April 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28421867/symptom-related-attributional-biases-in-schizophrenia-and-bipolar-disorder
#2
Nicole Sanford, Todd S Woodward
INTRODUCTION: Biases in causal attributions and evidence integration have been implicated in delusions, but have not been investigated simultaneously to examine additive or multiplicative effects. It was hypothesised that paranoid delusions would correlate with self-serving and personalising biases ("defence" model of paranoia), particularly when these biases were disconfirmed. METHODS: Constrained principal component analysis was used to investigate differences between schizophrenia patients (paranoid vs...
April 19, 2017: Cognitive Neuropsychiatry
https://www.readbyqxmd.com/read/28397118/proteomic-analysis-of-continuous-900-mhz-radiofrequency-electromagnetic-field-exposure-in-testicular-tissue-a-rat-model-of-human-cell-phone-exposure
#3
Masood Sepehrimanesh, Nasrin Kazemipour, Mehdi Saeb, Saeed Nazifi, Devra Lee Davis
Although cell phones have been used worldwide, some adverse and toxic effects were reported for this communication technology apparatus. To analyze in vivo effects of exposure to radiofrequency-electromagnetic field (RF-EMF) on protein expression in rat testicular proteome, 20 Sprague-Dawley rats were exposed to 900 MHz RF-EMF for 0, 1, 2, or 4 h/day for 30 consecutive days. Protein content of rat testes was separated by high-resolution two-dimensional electrophoresis using immobilized pH gradient (pI 4-7, 7 cm) and 12% acrylamide and identified by MALDI-TOF/TOF-MS...
April 10, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28385576/blood-transfusions-may-impair-endothelium-dependent-vasodilatation-during-coronary-artery-bypass-surgery
#4
Alessio Rungatscher, Elisabetta Milani, Cecilia Covajes, Seth Hallström, Leonardo Gottin, Gian Cesare Guidi, Giovanni Battista Luciani, Giuseppe Faggian
OBJECTIVE: The hemolytic product free-hemoglobin (fHb) reduces nitric oxide (NO) bioavailability. The present study aims to establish whether administration of different blood transfusions result in increased circulating fHb levels and NO consumption with effects on arterial NO-dependent blood flow in patients undergoing CABG surgery. METHODS: Ninety-five consecutive patients undergoing elective CABG surgery were prospectively divided in four groups based on blood transfusion requirements during surgery: stored blood cells (SBC, n...
April 4, 2017: Microvascular Research
https://www.readbyqxmd.com/read/28382669/effectiveness-of-a-playground-intervention-for-antisocial-prosocial-and-physical-activity-behaviors
#5
Carlene A Mayfield, Stephanie Child, Robert G Weaver, Nicole Zarrett, Michael W Beets, Justin B Moore
BACKGROUND: We examined the effectiveness of Peaceful Playgrounds™ (P2) to decrease antisocial behaviors (ASB) while increasing physical activity (PA) and prosocial behaviors (PSB) in elementary school children. METHODS: A longitudinal, cluster-randomized design was employed in 4 elementary school playgrounds where students (third to fifth) from 2 intervention and 2 control schools were observed during recess periods. The intervention included environmental changes (eg, marked surfaces) and student education...
May 2017: Journal of School Health
https://www.readbyqxmd.com/read/28350833/a-fast-and-frugal-algorithm-to-strengthen-diagnosis-and-treatment-decisions-for-catheter-associated-bacteriuria
#6
Aanand D Naik, Felicia Skelton, Amber B Amspoker, Russell A Glasgow, Barbara W Trautner
OBJECTIVES: Guidelines for managing catheter-associated urinary tract infection (CAUTI) and asymptomatic bacteria (ASB) are poorly translated into routine care due in part to cognitive diagnostic errors. This study determines if the accuracy for CAUTI and ASB diagnosis and treatment improves after implementation of a fast and frugal algorithm compared with traditional education methods. MATERIALS AND METHODS: A pre and post-intervention with contemporaneous comparison site involving inpatient and long term care wards at two regional Veterans Affairs Systems in United States...
2017: PloS One
https://www.readbyqxmd.com/read/28325856/prevalence-of-undiagnosed-asymptomatic-bacteriuria-and-associated-risk-factors-during-pregnancy-a-cross-sectional-study-at-two-tertiary-centres-in-cairo-egypt
#7
Mohamed Abdel-Aziz Elzayat, Ashton Barnett-Vanes, Mohamed Farag Elmorsy Dabour, Feng Cheng
BACKGROUND: The prevalence of asymptomatic bacteriuria (ASB) during pregnancy is poorly understood in Egypt-a country with a high birth rate. OBJECTIVES: To determine the prevalence of ASB among pregnant women booking at El Hussein and Sayed Galal Hospitals in Al-Azhar University in Egypt; and to observe the relationship between ASB prevalence and risk factors such as socioeconomic level and personal hygiene. SETTING: Obstetrics and gynaecology clinics of 2 university hospitals in the capital of Egypt...
March 21, 2017: BMJ Open
https://www.readbyqxmd.com/read/28321377/single-layer-repair-of-large-anterior-skull-base-defects-without-vascularized-mucosal-flap
#8
Frederick Yoo, Marilene B Wang, Marvin Bergsneider, Jeffrey D Suh
Objectives Bilateral anterior skull base (ASB) defects following endoscopic endonasal tumor resection are most commonly repaired utilizing multilayered reconstruction with a vascularized mucosal flap. Single-layer closure of large ASB defects has been described in the literature but this technique has yet to gain a widespread use. We report our experience with a series of patients who underwent reconstruction of large ASB defects using a single-layer intradural graft, without nasoseptal flaps. We also compared the use of acellular dermal matrix (AlloDerm, LifeCell, Branchburg, New Jersey, United States) or collagen matrix xenograft (Duramatrix, Stryker, Kalamazoo, Michigan, United States) as the graft biomaterial...
April 2017: Journal of Neurological Surgery. Part B, Skull Base
https://www.readbyqxmd.com/read/28293145/nursing-home-nurses-and-community-dwelling-older-adults-reported-knowledge-attitudes-and-behavior-toward-antibiotic-use
#9
Christine E Kistler, Anna Beeber, Sylvia Becker-Dreps, Kimberly Ward, Megan Meade, Brittany Ross, Philip D Sloane
BACKGROUND: Antibiotic overuse causes antibiotic resistance, one of the most important threats to human health. Older adults, particularly those in nursing homes, often receive antibiotics when they are not indicated. METHODS: To understand knowledge, attitudes, and behaviors of nursing home (NH) nurses and community-dwelling older adults towards antibiotic use, especially in clinical situations consistent with antibiotic overuse, we conducted a mixed-method survey in two NHs and one Family Medicine clinic in North Carolina, among English-speaking nurses and community-dwelling, cognitively intact adults aged 65 years or older...
2017: BMC Nursing
https://www.readbyqxmd.com/read/28292694/asb-clinical-biomechanics-award-winner-2016-assessment-of-gaze-stability-within-24-48hours-post-concussion
#10
Nicholas G Murray, Nathan R D'Amico, Douglas Powell, Megan E Mormile, Katelyn E Grimes, Barry A Munkasy, Russell K Gore, Rebecca J Reed-Jones
BACKGROUND: Approximately 90% of athletes with concussion experience a certain degree of visual system dysfunction immediately post-concussion. Of these abnormalities, gaze stability deficits are denoted as among the most common. Little research quantitatively explores these variables post-concussion. As such, the purpose of this study was to investigate and compare gaze stability between a control group of healthy non-injured athletes and a group of athletes with concussions 24-48hours post-injury...
March 6, 2017: Clinical Biomechanics
https://www.readbyqxmd.com/read/28264429/metabolic-effects-of-replacing-sugar-sweetened-beverages-with-artificially-sweetened-beverages-in-overweight-subjects-with-or-without-hepatic-steatosis-a-randomized-control-clinical-trial
#11
Vanessa Campos, Camille Despland, Vaclav Brandejsky, Roland Kreis, Philippe Schneiter, Chris Boesch, Luc Tappy
OBJECTIVE: Addition of fructose to the diet of normal weight and overweight subjects can increase postprandial plasma triglyceride and uric acid concentration. We, therefore, assessed whether replacing sugar-sweetened beverages (SSB) with artificially-sweetened beverages (ASB) in the diet of overweight and obese subjects would decrease these parameters. METHODS: Twenty-six participants of the REDUCS study, which assessed the effects of replacing SSB by ASB over 12 weeks on intra-hepatocellular lipid concentration, were included in this sub-analysis...
February 27, 2017: Nutrients
https://www.readbyqxmd.com/read/28260390/angiotensin-converting-enzyme-inhibitors-induced-angioedema-of-the-small-bowel-mimicking-postoperative-complication
#12
Caroline Lorenzoni Almeida Ghezzi, Tiago Leal Ghezzi, Oly Campos Corleta
BACKGROUND: Angioedema of the small bowel (ASB) is an extremely rare side effect of the angiotensin-converting enzyme inhibitors (ACEI). We present a case of ACEI-induced ASB mimicking postoperative complication. CASE REPORT: a woman who restarted ACEI after laparoscopic abdominoperineal resection of the rectum and anal canal has presented complaints of abdominal pain, nausea and vomiting, and non-acceptance of oral and enteral feeding. Computed tomography scan was performed to rule out surgical complications and has demonstrated an unexplained wall thickening of the proximal small bowel...
March 6, 2017: Revista Española de Enfermedades Digestivas
https://www.readbyqxmd.com/read/28209482/effectiveness-of-a-tailored-anterior-saturation-band-in-the-improvement-of-the-image-quality-of%C3%A2-pelvic-magnetic-resonance-for-assessing-rectal%C3%A2-cancer
#13
Chen-Chang Lee, Po-Chou Chen, Hong-Hwa Chen, Chung-Cheng Huang, Li-Han Lin, Shu-Hang Ng, Min-Chi Chen, Sheung-Fat Ko
PURPOSE: We sought to test the effectiveness of the application of a tailored anterior saturation band (ASB) to improve the image quality of pelvic magnetic resonance imaging (MRI) for assessing rectal cancer. METHODS: A total of 165 patients with MRI assessment of rectal cancer between 2013 and 2015 were included. The image quality scores (4-point scale: 1, nondiagnostic through 4, excellent) of MRI without and with tailored ASBs were compared. Sensitivity, specificity, positive and negative predictive values, and accuracy of pelvic MRIs with and without a tailored ASB for T-staging in 65 patients with direct surgery and 67 patients with chemoradiotherapy before surgery were evaluated...
January 25, 2017: Clinical Colorectal Cancer
https://www.readbyqxmd.com/read/28206859/evaluation-and-adaptation-of-attractive-toxic-sugar-baits-for-culex-tarsalis-and-culex-quinquefasciatus-control-in-the-coachella-valley-southern-california
#14
Whitney A Qualls, Jodi Scott-Fiorenzano, Gunter C Müller, Kristopher L Arheart, John C Beier, Rui-De Xue
The project goal was to determine how a new vector control strategy that targets the sugar-feeding behavior of mosquitoes, attractive toxic sugar baits (ATSBs), can be used to more effectively control West Nile virus (WNV) vectors in the Coachella Valley, California. Three laboratory studies were conducted to determine the utility of this method for control against Culex quinquefasciatus and Culex tarsalis : 1) efficacy evaluations of 2 formulations of ATSB, microencapsulated garlic oil, and a combination of microencapsulated garlic oil and 1% boric acid; 2) choice assays to determine the attractiveness of ATSB with the microencapsulated garlic oil against attractive sugar baits (ASB; the attractant alone; without toxin) and a 10% sucrose solution; and 3) vegetation efficacy tests on 3 common plant species in the Coachella Valley, Atriplex lentiformis, Tamarix ramosissima , and Pluchea sericea...
December 2016: Journal of the American Mosquito Control Association
https://www.readbyqxmd.com/read/28131446/human-exposure-assessment-of-different-arsenic-species-in-household-water-sources-in-a-high-risk-arsenic-area
#15
Hifza Rasheed, Paul Kay, Rebecca Slack, Yun Yun Gong, Annie Carter
Understanding arsenic speciation in water is important for managing the potential health risks associated with chronic arsenic exposure. Most arsenic monitoring studies to date have only measured total arsenic, with few looking at arsenic species. This study assessed 228 ground water sources in six unstudied villages in Pakistan for total, inorganic and organic arsenic species using ion chromatography inductively coupled plasma collision reaction cell mass spectrometry. The concentration levels approached 3090μgL(-1) (95% CI, 130...
April 15, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28112866/sweetened-beverage-intake-in-association-to-energy-and-sugar-consumption-and-cardiometabolic-markers-in-children
#16
P Seferidi, C Millett, A A Laverty
BACKGROUND: Artificially sweetened beverages (ASBs) are promoted as healthy alternatives to sugar-sweetened beverages (SSBs) in order to reduce sugar intake, but their effects on weight control and glycaemia have been debated. This study examines associations of SSBs and ASBs with energy and sugar intake and cardiometabolic measures. METHODS: One thousand six hundred eighty-seven children aged 4-18 participated in the National Diet and Nutrition Survey Rolling Programme (2008/9-2011/12) in the UK...
January 23, 2017: Pediatric Obesity
https://www.readbyqxmd.com/read/28088454/alpha-l-iduronidase-and-arylsulfatase-b-in-dried-blood-spots-on-filter-paper-biochemical-parameters-and-time-stability
#17
Ana Carolina Breier, Jaqueline Cé, Jamila Mezzalira, Vanessa V Daitx, Vitoria C Moraes, Mariana P S Goldim, Janice C Coelho
BACKGROUND: The goal of this study was to assess the biochemical parameters of the enzymes α-l-iduronidase (IDUA) and arylsulfatase B (ASB), which are deficient in mucopolysaccharidosis (MPS) I and VI, respectively, in dried blood spot (DBS) samples impregnated on filter paper. METHODS AND RESULTS: The optimal pH, Km, and Vmax of IDUA and ASB in DBS are hereby presented. After these analyses, the reference values for the activities of these enzymes in DBS with cutoff of 3...
January 11, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28088228/the-loss-of-function-mutations-and-down-regulated-expression-of-asb3-gene-promote-the-growth-and-metastasis-of-colorectal-cancer-cells
#18
Wu-Ying Du, Zhen-Hai Lu, Wen Ye, Xiang Fu, Yi Zhou, Chun-Mei Kuang, Jiang-Xue Wu, Zhi-Zhong Pan, Shuai Chen, Ran-Yi Liu, Wen-Lin Huang
BACKGROUND: Ankyrin repeat and SOCS box protein 3 (ASB3) is a member of ASB family and contains ankyrin repeat sequence and SOCS box domain. Previous studies indicated that it mediates the ubiquitination and degradation of tumor necrosis factor receptor 2 and is likely involved in inflammatory responses. However, its effects on oncogenesis are unclear. This study aimed to investigate the effects of ASB3 on the growth and metastasis of colorectal cancer (CRC). METHODS: We used next-generation sequencing or Sanger sequencing to detect ASB3 mutations in CRC specimens or cell lines, and used real-time quantitative polymerase chain reaction, Western blotting, and immunohistochemical or immunofluorescence assay to determine gene expression...
January 14, 2017: Chinese Journal of Cancer
https://www.readbyqxmd.com/read/28074617/soft-drink-intake-and-the-risk-of-metabolic-syndrome-a%C3%A2-systematic-review-and-meta-analysis
#19
REVIEW
Aditya Narain, Chun Shing Kwok, Mamas A Mamas
BACKGROUND: It is unclear whether consumption of sugar- or artificially sweetened beverages is independently associated with the development of metabolic syndrome. A systematic review and meta-analysis was performed to evaluate whether soft drink consumption is associated with the development of metabolic syndrome. METHODS: Medline and EMBASE were searched in November 2015 for studies which considered soft drink (sugar-sweetened beverage [SSB] and artificially sweetened beverage [ASB]) intake and risk of metabolic syndrome...
February 2017: International Journal of Clinical Practice
https://www.readbyqxmd.com/read/28069991/community-alcohol-partnerships-with-the-alcohol-industry-what-is-their-purpose-and-are-they-effective-in-reducing-alcohol-harms
#20
M Petticrew, N Douglas, P D'Souza, Y M Shi, M A Durand, C Knai, E Eastmure, N Mays
BACKGROUND: Local initiatives to reduce alcohol harms are common. One UK approach, Community Alcohol Partnerships (CAPs), involves partnerships between the alcohol industry and local government, focussing on alcohol misuse and anti-social behaviour (ASB) among young people. This study aimed to assess the evidence of effectiveness of CAPs. METHODS: We searched CAP websites and documents, and databases, and contacted CAPs to identify evaluations and summarize their findings...
January 9, 2017: Journal of Public Health
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