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Oriana Kreutzfeld, Katja Müller, Kai Matuschewski
Continuous stage conversion and swift changes in the antigenic repertoire in response to acquired immunity are hallmarks of complex eukaryotic pathogens, including Plasmodium species, the causative agents of malaria. Efficient elimination of Plasmodium liver stages prior to blood infection is one of the most promising malaria vaccine strategies. Here, we describe different genetically arrested parasites (GAPs) that have been engineered in Plasmodium berghei, P. yoelii and P. falciparum and compare their vaccine potential...
2017: Frontiers in Cellular and Infection Microbiology
Ariane Sadr-Nabavi, Juliane Ramser, Juliane Volkmann, Joerg Naehrig, Frank Wiesmann, Beate Betz, Heide Hellebrand, Stefanie Engert, Susanne Seitz, Rene Kreutzfeld, Takako Sasaki, Norbert Arnold, Rita Schmutzler, Marion Kiechle, Dieter Niederacher, Nadia Harbeck, Edgar Dahl, Alfons Meindl
EGF-containing fibulin-like extracellular matrix protein 1 (EFEMP1) was recently described as an antagonist of angiogenesis. Motivated by a strong dependence of tumor growth and metastasis on angiogenesis, we investigated the role of EFEMP1 in human breast cancer. We applied RNA microarray expression analysis and quantitative real-time PCR (QRT) in a total of 45 sporadic breast cancer tissues and found EFEMP1 down-regulation in 59% and 61% of the analyzed tissues, respectively. This down-regulation was confirmed on protein level...
April 1, 2009: International Journal of Cancer. Journal International du Cancer
Edgar Dahl, Ariane Sadr-Nabavi, Eva Klopocki, Beate Betz, Susanne Grube, Rene Kreutzfeld, Marina Himmelfarb, Han-Xiang An, Stephen Gelling, Irina Klaman, Bernd Hinzmann, Glen Kristiansen, Robert Grützmann, Ruprecht Kuner, Beate Petschke, Kerstin Rhiem, Kai Wiechen, Christine Sers, Otmar Wiestler, Achim Schneider, Heinz Höfler, Jörg Nährig, Manfred Dietel, Reinhold Schäfer, André Rosenthal, Rita Schmutzler, Matthias Dürst, Alfons Meindl, Dieter Niederacher
The identification of novel disease-associated genes in gynaecological tumours has important implications for understanding the process of tumourigenesis and the development of novel treatment regimens. cDNA libraries from disease tissues may represent a valuable source to identify such genes. Recently, a bio-informatic procedure based on an 'electronic Northern' approach was established to screen expressed sequence tag (EST) libraries for genes differentially expressed in tumour and normal tissues, and identified 450 candidate genes differentially expressed in breast and ovarian cancer...
January 2005: Journal of Pathology
S Flohé, J Börgermann, E Kreutzfelder, L Lim, R Flach, M Majetschak, U Obertacke, F U Schade
Cardiac surgery and polytrauma result in an impaired immune response as it can be demonstrated by a reduced endotoxin-stimulated TNF alpha production of whole blood cultures ex vivo. The immune-stimulating hematopoetic growth factor GM-CSF is in vitro capable to antagonize the suppressed immune function after trauma and cardiac surgery and, therefore, GM-CSF represents a potential therapeutic for immune suppressed states.
1998: Langenbecks Archiv Für Chirurgie. Supplement. Kongressband
Kerstin Rhiem, Annette Klein, Miriam Münch, Rene Kreutzfeld, Juliane Ramser, Eva Wardelmann, Gabriele Schackert, Andreas Von Deimling, Otmar D Wiestler, Rita K Schmutzler
Allelic imbalance constitutes a major mechanism of genetic aberrations in breast cancer and strongly indicates the involvement of tumor associated genes in the affected chromosomal regions. Preliminary results from our study indicated the existence of a tumor suppressor gene located on chromosomal arm 15q which may be involved in breast cancer progression.1 In the present study, 210 primary breast carcinomas, 30 metastases and 26 local recurrences from primary breast carcinomas have been analyzed with a panel of 18 highly polymorphic microsatellite markers spanning the chromosomal region 15q11-21...
August 10, 2003: International Journal of Cancer. Journal International du Cancer
U Preuss, R Kreutzfeld, K H Scheidtmann
SV40 large T antigen-induced primitive neuroectodermal tumors of the rat provide a model system to study induction and progression of primitive neuroectodermal tumors at the molecular level. A cell line derived from such a tumor reproducibly gave rise to malignant derivatives that ceased large T-antigen expression but harbored a mutant p53 allele with a common mutation at Cys(174) to Tyr (C174Y). To determine whether this p53 mutation contributes to tumor progression, we analyzed mutant C174Y functionally. Co-transfection experiments in Saos-2 cells with mutant or wild-type p53 and reporter genes linked to various p53-responsive promoters revealed that mutant C174Y failed to transcriptionally transactivate the Mdm2, Waf1, Cyclin G and Bax promoters...
October 15, 2000: International Journal of Cancer. Journal International du Cancer
A J Svendsen, J C Kreutzfeld, E B Lund, K O Kyvik, A Green
To provide contemporary figures of the incidence of childhood onset diabetes mellitus in Denmark, a prospective routine registration system has been established. The present study covered the population of children aged 0-14 years in four Danish counties, observed during the calendar years 1989 through 1993 concerning new cases of the disease. A total of 201 cases (113 boys and 88 girls) were registered, corresponding with a sex- and age-adjusted incidence rate of 17.4 per 100,000 person-years. Based on a validation analysis the study material is considered virtually complete...
February 24, 1997: Ugeskrift for Laeger
E G Orlova, L V Salimova
Long-term observations of five patients aged 40 to 70 years made it possible to diagnose Creutzfeld-Jacob's disease (CJD) in their life time. In three cases, the diagnosis of CJD was confirmed at autopsy; one patient died at home. The works of I. Kreutzfeld and A. Jacob and the authors' observations confirm that the disease lasts 4-5 years or more. Marked manifestations of the disease were observed for two years. Some differential diagnostic criteria are presented permitting the differentiation of CJD from other atrophic processes described by Alzheimer, Pick and others...
1984: Zhurnal Nevropatologii i Psikhiatrii Imeni S.S. Korsakova
K L Kreutzfeld, K Y Lei, M D Bregman, F L Meyskens
Zinc inhibited the colony formation of Cloudman S-91 murine melanoma cells in a dose dependent manner with an ID50 of 3.4 ug/ml. Total inhibition of the melanoma colony-forming units occurred at a zinc concentration of 4.42 ug/ml. In the presence of dexamethasone the ID50 for zinc inhibition was reduced by 49% and total inhibition of anchorage-independent growth occurred at the achievable in vivo zinc concentration of 3.0 ug/ml. Dexamethasone and zinc in combination effected a greater than additive inhibition of the murine melanoma colony-forming units...
March 4, 1985: Life Sciences
M M Marwan, Z A Abdel Malek, K L Kreutzfeld, M E Hadley, B C Wilkes, V J Hruby, A M Castrucci
alpha-Melanocyte-stimulating hormone (alpha-MSH, alpha-melanotropin), [Nle4,D-Phe7]-alpha-MSH and related fragment analogues, Ac-[Nle4,D-Phe7]-alpha-MSH4-11-NH2 and Ac-[Nle4,D-Phe7]-alpha-MSH4-10-NH2, were studied for their ability to stimulate tyrosinase activity in Cloudman S91 mouse melanoma cells in tissue culture. All of the melanotropins stimulated tyrosinase activity in a dose-dependent manner. [Nle4,D-Phe7]-alpha-MSH was about 100 times more active than alpha-MSH as determined from the minimal effective dose (MED) required to activate the enzyme above control (basal) levels...
July 1985: Molecular and Cellular Endocrinology
J T Bagnara, K L Kreutzfeld, P J Fernandez, A C Cohen
No abstract text is available yet for this article.
1988: Pigment Cell Research
D G Klemes, K L Kreutzfeld, M E Hadley, W L Cody, V J Hruby
Ac-[Nle4, D-Phe7]-alpha-MSH4-9-NH2 and Ac-[Nle4]-alpha-MSH4-9-NH2, fragment analogs of the tridecapeptide, alpha-melanocyte stimulating hormone (alpha-MSH, alpha-melanotropin), were synthesized. The potency and prolonged activity of the analogs were compared to alpha-MSH in several melanotropin bioassays. The D-Phe-containing hexapeptide was 10 times more active than alpha-MSH in stimulating melanoma tyrosinase activity. This analog was also 10-fold more potent than alpha-MSH in the lizard skin bioassay and about 10-fold less active in the frog skin bioassay...
June 13, 1986: Biochemical and Biophysical Research Communications
Z A Abdel Malek, K L Kreutzfeld, M E Hadley, M D Bregman, V J Hruby, F L Meyskens
Cell density is a factor that affects the capacity of Cloudman S91 melanoma cells to respond to melanotropins in monolayer culture. Continuous exposure of melanoma cells to alpha-melanotropin or its potent analog [Nle4, D-Phe7]-alpha-MSH, resulted in maximal stimulation of tyrosinase after 2 d of treatment, but the magnitude of stimulation decreased thereafter despite the continued presence of the melanotropins. However, when melanoma cells continually exposed to melanotropins were subcultured to an initial low cell density and maintained in contact with alpha-MSH or [Nle4, D-Phe7]-alpha-MSH (long-term culture), tyrosinase activity was rapidly restored and greatly enhanced...
February 1986: In Vitro Cellular & Developmental Biology: Journal of the Tissue Culture Association
M E Hadley, Z A Abdel Malek, M M Marwan, K L Kreutzfeld, V J Hruby
The superpotent and ultraprolonged melanotropic properties of an alpha-melanotropin analog, [Nle4, D-Phe7]-alpha-MSH, were investigated in a Cloudman S91 (CCL 53.1) melanoma cell line. [Nle4, D-Phe7]-alpha-MSH is 100-fold more effective than the native hormone, alpha-melanocyte stimulating hormone (alpha-MSH), in stimulating melanoma cell tyrosinase activity, as determined from their minimum effective doses (10(-11)M and 10(-9)M, respectively). [Nle4, D-Phe7]-alpha-MSH also exhibits a more sustained effect than alpha-MSH on tyrosinase after removal of the melanotropins from the incubation medium...
1985: Endocrine Research
Z A Abdel Malek, K L Kreutzfeld, M M Marwan, M E Hadley, V J Hruby, B C Wilkes
alpha-Melanotropin (alpha-melanocyte stimulating hormone, alpha-MSH) stimulates tyrosinase activity in Cloudman S91 murine melanoma cells. Three [Nle4, D-Phe7]-substituted alpha-melanotropin analogues, [Nle4, D-Phe7]-alpha-MSH, Ac-[Nle4, D-Phe7]-alpha-MSH4-11-NH2, and Ac-[Nle4, D-Phe7]-alpha-MSH4-10-NH2, are at least 100-fold more effective than alpha-MSH in stimulating melanoma tyrosinase, the rate-limiting enzyme in melanin biosynthesis. These [Nle4, D-Phe7]-substituted melanotropin analogues induce tyrosinase activity in melanoma cells with shorter contact times than required by the native hormone, alpha-MSH...
October 1985: Cancer Research
D N Chaturvedi, V J Hruby, A M Castrucci, K L Kreutzfeld, M E Hadley
The fluorescein-labeled melanotropin [N alpha-chlorotriazinylaminofluorescein-Ser1,Nle4,D-Phe 7]-alpha-MSH, was prepared by solid-phase techniques of peptide synthesis. The biological actions of this analogue were determined in several melanocyte bioassays and were compared with the parent peptide [Nle4,D-Phe7]-alpha-MSH and the native hormone alpha-MSH. The fluorescein compound was a superpotent agonist with approximately 10 times more activity than alpha-MSH in both the frog and the lizard skin bioassays...
March 1985: Journal of Pharmaceutical Sciences
B V Dawson, M E Hadley, K Kreutzfeld, R T Dorr, V J Hruby, F Al-Obeidi, S Don
We previously reported that topical application of [Nle4,D-Phe7]alpha-MSH, a superpotent analogue of alpha-melanocyte stimulating hormone, to mice induces a darkening of follicular melanocytes throughout the skin. We now report that the melanotropin analogue can be delivered across mouse but not rat skin in an in vitro model system. Passage of the analogue from the topically applied vehicle (polyethylene glycol) across the skin into a subcutaneous receiving vessel was demonstrated by both bioassay as well as by radioimmunoassay...
1988: Life Sciences
K L Kreutzfeld, T Fukuzawa, J T Bagnara
No abstract text is available yet for this article.
March 1989: Pigment Cell Research
W Lösche, E Michel, K Lull, B Kreutzfeld, S Heptinstall, U Till
Incubation of citrated or heparinized blood samples with 25 or 50 mmoles/l glucose for 1 hour at 37 degrees C results in a decrease in the osmotic and mechanical resistance of red blood cells as well as in an increase in the red blood cell volume measured as increase in haematocrit. Fructose, but not sorbitol or saccharose has effects on red blood cells that are similar to those of glucose. It is discussed that an uptake of glucose into red blood cells with subsequent water influx results in swelling and decreased stability of the cells...
1989: Zeitschrift Für Medizinische Laboratoriumsdiagnostik
K L Kreutzfeld, J T Bagnara
No abstract text is available yet for this article.
January 1989: Pigment Cell Research
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