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Adipose stromal vascular fraction

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https://www.readbyqxmd.com/read/29920999/anti-apoptotic-effects-of-adipose-derived-adherent-stromal-cells-in-mesenchymal-stem-cells-exposed-to-oxidative-stress
#1
Sunhye Shin, Jung-Won Choi, Soyeon Lim, Seahyoung Lee, Eun-Young Jun, Hyun-Min Sun, Il-Kwon Kim, Hoon-Bum Lee, Sang Woo Kim, Ki-Chul Hwang
Adipose-derived stromal vascular fractions (SVFs) are a heterogeneous collection of cells, and their regenerative modality has been applied in various animal experiments and clinical trials. Despite the attractive advantages of SVFs in clinical interventions, the recent status of clinical studies involving the application of SVFs in many diseases has not been fully evaluated. Mesenchymal stem cells (MSCs) are multipotent stromal cells that can differentiate into a variety of cell types despite their low numbers in heart tissue...
June 19, 2018: Cell Biochemistry and Function
https://www.readbyqxmd.com/read/29909687/comparative-efficacy-of-autologous-stromal-vascular-fraction-and-autologous-adipose-derived-mesenchymal-stem-cells-combined-with-hyaluronic-acid-for-the-treatment-of-sheep-osteoarthritis
#2
Xiaoteng Lv, Jiyin He, Xue Zhang, Xuan Luo, Na He, Zhongwei Sun, Huitang Xia, Victor Liu, Li Zhang, Xiangming Lin, Liping Lin, Huabin Yin, Dong Jiang, Wei Cao, Richard Wang, Guangdong Zhou, Wen Wang
The current study explored whether intra-articular (IA) injection of autologous adipose mesenchymal stem cells (ASCs) combined with hyaluronic acid (HA) achieved better therapeutic efficacy than autologous stromal vascular fraction (SVF) combined with HA to prevent osteoarthritis (OA) progression and determined how long autologous ASCs combined with HA must remain in the joint to observe efficacy. OA models were established by performing anterior cruciate ligament transection (ACLT) and medial meniscectomy (MM)...
January 1, 2018: Cell Transplantation
https://www.readbyqxmd.com/read/29905790/characterization-of-the-tissue-and-stromal-cell-components-of-micro-superficial-enhanced-fluid-fat-injection-micro-seffi-for-facial-aging-treatment
#3
Martina Rossi, Barbara Roda, Silvia Zia, Ilaria Vigliotta, Chiara Zannini, Francesco Alviano, Laura Bonsi, Andrea Zattoni, Pierluigi Reschiglian, Alessandro Gennai
Background: New microfat preparations provide material suitable for use as a regenerative filler for different facial areas. To support the development of new robust techniques for regenerative purposes, the cellular content of the sample should be considered. Objectives: To evaluate the stromal vascular fraction (SVF) cell components of micro-superficial enhanced fluid fat injection (SEFFI) samples via a technique to harvest re-injectable tissue with minimum manipulation...
June 14, 2018: Aesthetic Surgery Journal
https://www.readbyqxmd.com/read/29891687/macrophage-phenotype-and-bioenergetics-are-controlled-by-oxidized-phospholipids-identified-in-lean-and-obese-adipose-tissue
#4
Vlad Serbulea, Clint M Upchurch, Michael S Schappe, Paxton Voigt, Dory E DeWeese, Bimal N Desai, Akshaya K Meher, Norbert Leitinger
Adipose tissue macrophages (ATMs) adapt their metabolic phenotype either to maintain lean tissue homeostasis or drive inflammation and insulin resistance in obesity. However, the factors in the adipose tissue microenvironment that control ATM phenotypic polarization and bioenergetics remain unknown. We have recently shown that oxidized phospholipids (OxPL) uniquely regulate gene expression and cellular metabolism in Mox macrophages, but the presence of the Mox phenotype in adipose tissue has not been reported...
June 11, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29882687/gender-and-age-related-cell-compositional-differences-in-c57bl-6-murine-adipose-tissue-stromal-vascular-fraction
#5
Trivia Frazier, Stephen Lee, Annie Bowles, Julie Semon, Bruce Bunnell, Xiying Wu, Jeffrey Gimble
Adipose tissue is now recognized as a functional organ that contains cellular heterogeneity and diversity within anatomical depots. The stromal vascular fraction (SVF) of adipose contains endothelial progenitors, fibroblasts, lymphocytes, monocyte/macrophages, pericytes, pre-adipocytes, and stromal/stem cells, among others. In recent years, there has been a growing appreciation of the influence of age and gender in the field of stem cell biology. Yet few studies have evaluated the influence of biological age or sex on either SVF cell heterogeneity or immunophenotype...
June 8, 2018: Adipocyte
https://www.readbyqxmd.com/read/29876178/a-novel-porcine-model-for-future-studies-of-cell-enriched-fat-grafting
#6
Bo S Rasmussen, Celine L Sørensen, Peter V Vester-Glowinski, Mikkel Herly, Sorel Kurbegovic, Mathias Ørholt, Jesper D Svalgaard, Stig-Frederik T Kølle, Annemarie T Kristensen, Maj-Lis M Talman, Krzysztof T Drzewiecki, Anne Fischer-Nielsen
Background: Cell-enriched fat grafting has shown promising results for improving graft survival, although many questions remain unanswered. A large animal model is crucial for bridging the gap between rodent studies and human trials. We present a step-by-step approach in using the Göttingen minipig as a model for future studies of cell-enriched large volume fat grafting. Methods: Fat grafting was performed as bolus injections and structural fat grafting. Graft retention was assessed by magnetic resonance imaging after 120 days...
April 2018: Plastic and Reconstructive Surgery. Global Open
https://www.readbyqxmd.com/read/29799877/aortic-carboxypeptidase-like-protein-enhances-adipose-tissue-stromal-progenitor-differentiation-into-myofibroblasts-and-is-upregulated-in-fibrotic-white-adipose-tissue
#7
Mike Jager, Mi-Jeong Lee, Chendi Li, Stephen R Farmer, Susan K Fried, Matthew D Layne
White adipose tissue expands through both adipocyte hypertrophy and hyperplasia and it is hypothesized that fibrosis or excess accumulation of extracellular matrix within adipose tissue may limit tissue expansion contributing to metabolic dysfunction. The pathways that control adipose tissue remodeling are only partially understood, however it is likely that adipose tissue stromal and perivascular progenitors participate in fibrotic remodeling and also serve as adipocyte progenitors. The goal of this study was to investigate the role of the secreted extracellular matrix protein aortic carboxypeptidase-like protein (ACLP) on adipose progenitor differentiation in the context of adipose tissue fibrosis...
2018: PloS One
https://www.readbyqxmd.com/read/29793088/decellularized-and-matured-esophageal-scaffold-for-circumferential-esophagus-replacement-proof-of-concept-in-a-pig-model
#8
Guillaume Luc, Guillaume Charles, Caroline Gronnier, Magali Cabau, Charlotte Kalisky, Mallory Meulle, Reine Bareille, Samantha Roques, Lionel Couraud, Johanna Rannou, Laurence Bordenave, Denis Collet, Marlène Durand
Surgical resection of the esophagus requires sacrificing a long portion of it. Its replacement by the demanding gastric pull-up or colonic interposition techniques may be avoided by using short biologic scaffolds composed of decellularized matrix (DM). The aim of this study was to prepare, characterize, and assess the in vivo remodeling of DM and its clinical impact in a preclinical model. A dynamic chemical and enzymatic decellularization protocol of porcine esophagus was set up and optimized. The resulting DM was mechanically and biologically characterized by DNA quantification, histology, and histomorphometry techniques...
May 17, 2018: Biomaterials
https://www.readbyqxmd.com/read/29781904/adipose-stromal-vascular-fraction-gel-grafting-a-new-method-for-tissue-volumization-and-rejuvenation
#9
Yao Yao, Junrong Cai, Pan Zhang, Yunjun Liao, Yi Yuan, Ziqing Dong, Feng Lu
BACKGROUND: The clinical outcomes of fat grafting vary and are technique-dependent. Stromal vascular fraction (SVF) gel is a novel, mechanically processed fat product with high concentrations of adipose tissue-derived stem cells and other SVF cells. This study evaluated the volumization and rejuvenation effects of SVF-gel. OBJECTIVE: This study evaluated the volumization and rejuvenation effects of SVF-gel. METHODS: This retrospective, single-center study included 126 patients who underwent SVF-gel grafting and 78 who underwent conventional lipoinjection for various indications from March 2015 to February 2017...
May 16, 2018: Dermatologic Surgery: Official Publication for American Society for Dermatologic Surgery [et Al.]
https://www.readbyqxmd.com/read/29766760/osteoinduction-by-ex-vivo-bone-morphogenetic-protein-gene-delivery-is-independent-of-cell-type
#10
Loek Loozen, Moyo C Kruyt, Angela Vandersteen, Angela H M Kragten, Michiel Croes, F Cumhur Oner, Jacqueline Alblas
Ex vivo non-viral gene delivery of bone-inductive factors has the potential to heal bone defects. Due to their inherent role in new bone formation, multipotent stromal cells (MSCs) have been studied as the primary target cell for gene delivery in a preclinical setting. The relative contribution of autocrine and paracrine mechanisms, and the need of osteogenic cells, remains unclear. This study investigates the contribution of MSCs as producer of transgenic BMPs and to what extent the seeded MSCs participate in actual osteogenesis...
May 16, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29764513/cellular-therapy-with-human-autologous-adipose-derived-adult-cells-of-stromal-vascular-fraction-for-alopecia-areata
#11
Rami Anderi, Nehman Makdissy, Albert Azar, Francine Rizk, Aline Hamade
BACKGROUND: Most common forms of hair loss (alopecia) are caused by aberrant hair follicle cycling and changes in hair follicle morphology. However, current treatments for alopecia do not specifically target these processes. Adipose-derived stromal vascular cells (ADSVCs) that can be harvested from fat cells are one of the latest breakthroughs in the aesthetic field. The potential use of stem cell-based therapies (SCBT) for the repair and regeneration of various tissues and organs offers a paradigm shift that may provide alternative therapeutic solutions, which can be applied to prevent hair loss...
May 15, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29753026/chronic-hyperinsulinemia-promotes-meta-inflammation-and-extracellular-matrix-deposition-in-adipose-tissue-implications-of-nitric-oxide
#12
Durgesh Kumar, Kripa Shankar, Saraswati Patel, Abhishek Gupta, Salil Varshney, Sanchita Gupta, Sujith Rajan, Ankita Srivastava, Achchhe Lal Vishwakarma, Anil N Gaikwad
Various imperative studies support the notion that hyperinsulinemia (HI) itself serves as the common link between adipose tissue inflammation (ATI) and metabolic syndrome. However, the contribution of HI mediated ATI and its metabolic consequences are yet to be explored. We induced chronic HI per se in mice by administration of exogenous insulin for 8 weeks through mini-osmotic pumps. For the reduction of circulating insulin in response to excess calorie intake, we have partially ablated β-cells by using streptozotocin (STZ) in the diet-induced obesity (DIO) and genetic mice models (db/db)...
May 10, 2018: Molecular and Cellular Endocrinology
https://www.readbyqxmd.com/read/29751821/improved-gmp-compliant-approach-to-manipulate-lipoaspirates-to-cryopreserve-stromal-vascular-fraction-and-to-expand-adipose-stem-cells-in-xeno-free-media
#13
Francesco Agostini, Francesca Maria Rossi, Donatella Aldinucci, Monica Battiston, Elisabetta Lombardi, Stefania Zanolin, Samuele Massarut, Pier Camillo Parodi, Alessandro Da Ponte, Giovanni Tessitori, Barbara Pivetta, Cristina Durante, Mario Mazzucato
BACKGROUND: The stromal vascular fraction (SVF) derived from adipose tissue contains adipose-derived stromal/stem cells (ASC) and can be used for regenerative applications. Thus, a validated protocol for SVF isolation, freezing, and thawing is required to manage product administration. To comply with Good Manufacturing Practice (GMP), fetal bovine serum (FBS), used to expand ASC in vitro, could be replaced by growth factors from platelet concentrates. METHODS: Throughout each protocol, GMP-compliant reagents and devices were used...
May 11, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29745446/effects-of-vegf-ang-1-pla-nano-sustained-release-microspheres-on-proliferation-and-differentiation-of-adscs
#14
Yucang He, Zihao Li, Zhuojie Chen, Xiaofang Yu, Ziwan Ji, Jingping Wang, Yao Qian, Liqun Li
The improvement of fat graft viability might depend on the presence of multipotent resident adipose derived stem cells (ADSCs) which is the important component of stromal vascular fraction (SVF). Vascular endothelial growth factor (VEGF) and angiogenin-1 (Ang-1) are responsible for neovascularization. However, their half-life is too short to produce a biological effect. We thus investigated whether VEGF-ANG-1-polylactic acid (PLA) microspheres could enhance the angiogenic properties of ADSCs. PLA microspheres containing VEGF and ANG-1 were prepared by in vitro ultrasonic emulsification and characterized according to their encapsulation efficiency (EE), drug-loading rate (DL), particle size and drug release...
May 10, 2018: Cell Biology International
https://www.readbyqxmd.com/read/29738484/human-macrophages-preferentially-infiltrate-the-superficial-adipose-tissue
#15
Giuseppe Cappellano, Evi M Morandi, Johannes Rainer, Philipp Grubwieser, Katharina Heinz, Dolores Wolfram, David Bernhard, Susanne Lobenwein, Gerhard Pierer, Christian Ploner
Human abdominal subcutaneous adipose tissue consists of two individual layers—the superficial adipose tissue (SAT) and deep adipose tissue (DAT)—separated by the Scarpa’s fascia. The present study focuses on the analysis of morphological and immunological differences of primary adipocytes, adipose-derived stem cells (ASC), and tissue-infiltrating immune cells found in SAT and DAT. Adipocytes and stromal vascular fraction (SVF) cells were isolated from human SAT and DAT specimens and phenotypically characterized by in vitro assays...
May 8, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29737535/neural-differentiation-of-human-adipose-derived-mesenchymal-stem-cells-induced-by-glial-cell-conditioned-media
#16
Debora Lo Furno, Giuliana Mannino, Rosario Giuffrida, Elisa Gili, Carlo Vancheri, Maria S Tarico, Rosario E Perrotta, Rosalia Pellitteri
Adipose-derived mesenchymal stem cells (ASCs) may transdifferentiate into cells belonging to mesodermal, endodermal, and ectodermal lineages. The aim of this study was to verify whether a neural differentiation of ASCs could be induced by a conditioned medium (CM) obtained from cultures of olfactory ensheathing cells (OECs) or Schwann cells (SCs). ASCs were isolated from the stromal vascular fraction of adipose tissue and expanded for 2-3 passages. They were then cultured in OEC-CM or SC-CM for 24 hr or 7 days...
May 8, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29722764/platelet-rich-plasma-and-mesenchymal-stem-cells-exciting-but-%C3%A2-are-we-there-yet
#17
Isabel Andia, Jose I Martin, Nicola Maffulli
Joint conditions incapacitate free movement driving to a sedentary lifestyle, a major risk factor for chronic diseases. Regenerative procedures, involving the use of mesenchymal stem/stromal cells along with platelet-rich plasma (PRP), can help patients with these conditions. We describe the main characteristics of cellular products (bone marrow concentrate, stromal vascular fraction of adipose tissue, and mesenchymal stem/stromal cells derived from these tissues), and the potential benefits of combination with PRP in 3 scenarios: PRP lysates used during laboratory cell expansion; PRP to prime cellular products or the host tissue before cell implantation; PRP used as a vehicle for cell transplantation and to provide trophic signals...
June 2018: Sports Medicine and Arthroscopy Review
https://www.readbyqxmd.com/read/29715071/accelerated-healing-of-a-diabetic-foot-ulcer-using-autologous-stromal-vascular-fraction-suspended-in-platelet-rich-plasma
#18
Triantafyllos Didangelos, George Koliakos, Kokkona Kouzi, George Arsos, Katerina Kotzampassi, Konstantinos Tziomalos, Dimitrios Karamanos, Apostolos I Hatzitolios
We describe the case of a Type I diabetic patient with a refractory foot ulcer that remained unhealed for 2 years despite conventional therapy. Autologous adipose-derived stromal vascular fraction suspended in autologous platelet-rich plasma was applied to the wound, which completely healed within 1 month. The wound remained closed with no complications for a 2-year follow-up. Reporting of this and similar cases may lead to larger clinical trials that will prove the efficacy of this therapy that may offer accelerated healing and lessen the financial burden of more expensive therapeutic modalities...
April 2018: Regenerative Medicine
https://www.readbyqxmd.com/read/29713784/limited-evidence-for-adipose-derived-stem-cell-therapy-on-the-treatment-of-osteoarthritis
#19
REVIEW
Eoghan T Hurley, Youichi Yasui, Arianna L Gianakos, Dexter Seow, Yoshiharu Shimozono, Gino M M J Kerkhoffs, John G Kennedy
PURPOSE: The purpose of this systematic review is to evaluate the effects of adipose derived mesenchymal stem cells (ADSCs) in the treatment of osteoarthritis (OA) in the clinical setting. METHODS: A literature search was performed in the MEDLINE, EMBASE, and The Cochrane Library Database up to January 2017 for inclusion and exclusion criteria. Criteria for inclusion were clinical studies demonstrating the effects of ADSCs on OA, and written in English. The following variables were analyzed: donor site, volume of adipose tissue, preparation of ADSCs, clinical outcomes, and complication rate...
April 30, 2018: Knee Surgery, Sports Traumatology, Arthroscopy: Official Journal of the ESSKA
https://www.readbyqxmd.com/read/29705699/transplantation-of-periaortic-adipose-tissue-inhibits-atherosclerosis-in-apoe-mice-by-evoking-tgf-%C3%AE-1-mediated-anti-inflammatory-response-in-transplanted-graft
#20
Kensuke Terada, Hiroyuki Yamada, Masakazu Kikai, Noriyuki Wakana, Keita Yamamoto, Naotoshi Wada, Shinichiro Motoyama, Makoto Saburi, Takeshi Sugimoto, Daisuke Irie, Taku Kato, Hiroyuki Kawahito, Daisuke Kami, Takehiro Ogata, Satoaki Matoba
Perivascular adipose tissue (PAT) is associated with vascular homeostasis; however, its causal effect on atherosclerosis currently remains undefined. Here, we investigated the effect of experimental PAT transplantation on atherosclerosis. The thoracic periaortic adipose tissue (tPAT) was dissected from 16-week-old wild-type mice and transplanted over the infrarenal aorta of 20-week-old apoE deficient (apoE-/- ) mice fed high-cholesterol diet for 3 months. Oil-red O staining after 4 weeks showed a significant 20% decrease in the atherosclerotic lesion of suprarenal aorta compared with that of sham control mice, while that of infrarenal aorta showed no difference between the two groups...
June 18, 2018: Biochemical and Biophysical Research Communications
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