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Adipose stromal vascular fraction

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https://www.readbyqxmd.com/read/28230922/adipose-derived-aldehyde-dehydrogenase-expressing-cells-promote-dermal-regenerative-potential-with-collagen-glycosaminoglycan-scaffold
#1
Hajime Matsumine, Kazuyuki Numakura, Satoshi Tsunoda, Huan Wang, Rui Matsumine, Mihail Climov, Giorgio Giatsidis, Vikas P Sukhatme, Dennis P Orgill
Aldehyde dehydrogenase (ALDH) is an enzyme that plays an important role in retinoid metabolism and highly expressed in stem cells. This study isolated ALDH-expressing cells from subcutaneous adipose tissue and investigated their potential to enhance healing in a full-thickness skin wound in rats by co-implanting them with collagen-glycosaminoglycan (c-GAG) scaffolds. ALDH-positive cells were isolated by a fluorescence-activated cell sorting technique from Lewis rat's stromal-vascular-fraction (SVF) and transplanted with c-GAG scaffolds in a rat full-thickness skin wound model...
November 7, 2016: Wound Repair and Regeneration
https://www.readbyqxmd.com/read/28229415/isolation-of-stromal-stem-cells-from-adipose-tissue
#2
Maria Prat, Francesca Oltolina, Silvia Antonini, Andrea Zamperone
Adipose tissue has been shown to be particularly advantageous as source of mesenchymal stem cells (MSCs), because of its easy accessibility, and the possibility of obtaining stem cells in high yields. MSCs are obtained from the so-called Stromal Vascular Fraction, (SVF), exploiting their property of adhering to plastic surfaces and can be further purified by positive or negative immunomagnetic selection with appropriately chosen antibodies. These cells (Stromal Stem Cells, SSCs) can then be directly analyzed, frozen in liquid nitrogen, or expanded for further applications, e...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28228728/perivascular-adipose-tissue-s-impact-on-norepinephrine-induced-contraction-of-mesenteric-resistance-arteries
#3
Nadia Ayala-Lopez, Janice M Thompson, Stephanie W Watts
Background: Perivascular adipose tissue (PVAT) can decrease vascular contraction to NE. We tested the hypothesis that metabolism and/or uptake of vasoactive amines by mesenteric PVAT (MPVAT) could affect NE-induced contraction of the mesenteric resistance arteries. Methods: Mesenteric resistance vessels (MRV) and MPVAT from male Sprague-Dawley rats were used. RT-PCR and Western blots were performed to detect amine metabolizing enzymes. The Amplex® Red Assay was used to quantify oxidase activity by detecting the oxidase reaction product H2O2 and the contribution of PVAT on the mesenteric arteries' contraction to NE was measured by myography...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28222464/regulation-of-dipeptidyl-peptidase-4-its-substrate-chemokines-and-their-receptors-in-adipose-tissue-of-ob-ob-mice
#4
Jihoon Shin, Atsunori Fukuhara, Toshiharu Onodera, Chieko Yokoyama, Michio Otsuki, Iichiro Shimomura
The physiological function of DPP-4 in proteolytic inactivation of incretins has been well established, however, there is limited information on the expression and the significance of DPP-4 in white adipose tissue with regard to obesity. The objective of the work was to reveal the expression and regulation of DPP-4 in adipocytes and compare the expression and activity of DPP-4 in white adipose tissue and several other organs such as the liver, muscle and kidney. We also investigated the gene expression levels of DPP-4 substrate chemokines, and their receptors in white adipose tissue...
February 21, 2017: Hormone and Metabolic Research, Hormon- und Stoffwechselforschung, Hormones et Métabolisme
https://www.readbyqxmd.com/read/28209525/stromal-vascular-fraction-shows-robust-wound-healing-through-high-chemotactic-and-epithelialization-property
#5
Dong-Sic Chae, Seongho Han, Mina Son, Sung-Whan Kim
BACKGROUND: Although human stromal vascular fraction (SVF) has been regarded as an attractive stem cell source, its therapeutic mechanism in wound healing has not been fully elucidated. AIMS: In this study, we investigated the molecular characteristics and therapeutic property of SVF for wound healing. METHODS: Microarray data showed that SVF cells are enriched with a higher level of wound healing or epithelium development-related genes and micro RNA...
February 13, 2017: Cytotherapy
https://www.readbyqxmd.com/read/28186685/concise-review-fat-and-furious-harnessing-the-full-potential-of-adipose-derived-stromal-vascular-fraction
#6
Jordan A Dykstra, Tiffany Facile, Ryan J Patrick, Kevin R Francis, Samuel Milanovich, Jill M Weimer, Daniel J Kota
Due to their capacity to self-renew, proliferate and generate multi-lineage cells, adult-derived stem cells offer great potential for use in regenerative therapies to stop and/or reverse degenerative diseases such as diabetes, heart failure, Alzheimer's disease and others. However, these subsets of cells can be isolated from different niches, each with differing potential for therapeutic applications. The stromal vascular fraction (SVF), a stem cell enriched and adipose-derived cell population, has garnered interest as a therapeutic in regenerative medicine due to its ability to secrete paracrine factors that accelerate endogenous repair, ease of accessibility and lack of identified major adverse effects...
January 6, 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28177263/isolation-of-adipose-derived-stromal-vascular-fraction-cells-using-a-novel-point-of-care-device-cell-characterization-and-review-of-the-literature
#7
James Christian Brown, Hulan Shang, Ying Li, Ning Yang, Nikita Patel, Adam J Katz
The GID SVF-1 and GID SVF-2 are closed-system, disposable, scalable cellular isolation devices designed for isolating the human adipose-derived stromal vascular fraction (hSVF) from lipoaspirates at the point-of care. The purpose of this study was to characterize the device performance with respect to cell yield and viability of the hSVF, as well as the hSVF purity and cellular composition. Our results demonstrate that adipose-derived SVF can be safely obtained using both devices and standardized methods, yielding cells that are free of bacterial contaminants as measured by endotoxin levels, gram stain and culture...
February 8, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28174981/op008-an-innovative-treatment-for-refractory-perianal-fistulas-in-crohn-s-disease-local-micro-reinjection-of-autologous-fat-and-adipose-derived-stromal-vascular-fraction
#8
M Serrero, C Philandrianos, C Visee, J Veran, P Orsoni, F Sabatier, J-C Grimaud
No abstract text is available yet for this article.
February 1, 2017: Journal of Crohn's & Colitis
https://www.readbyqxmd.com/read/28170179/comparative-clinical-observation-of-arthroscopic-microfracture-in-the-presence-and-absence-of-a-stromal-vascular-fraction-injection-for-osteoarthritis
#9
Phu Dinh Nguyen, Tung Dang-Xuan Tran, Huynh Ton-Ngoc Nguyen, Hieu Trung Vu, Phuong Thi-Bich Le, Nhan Lu-Chinh Phan, Ngoc Bich Vu, Ngoc Kim Phan, Phuc Van Pham
Osteoarthritis (OA) is a degenerative cartilage disease that is characterized by a local inflammatory reaction. Consequently, many studies have been performed to identify suitable prevention and treatment interventions. In recent years, both arthroscopic microfracture (AM) and stem cell therapy have been used clinically to treat OA. This study aimed to evaluate the clinical effects of AM in the presence and absence of a stromal vascular fraction (SVF) injection in the management of patients with OA. Thirty patients with grade 2 or 3 (Lawrence scale) OA of the knee participated in this study...
January 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28165009/acellular-urethra-bioscaffold-decellularization-of-whole-urethras-for-tissue-engineering-applications
#10
Irina N Simões, Paulo Vale, Shay Soker, Anthony Atala, Daniel Keller, Rute Noiva, Sandra Carvalho, Conceição Peleteiro, Joaquim M S Cabral, Daniel Eberli, Cláudia L da Silva, Pedro M Baptista
Patients with stress urinary incontinence mainly suffer from malfunction of the urethra closure mechanism. We established the decellularization of porcine urethras to produce acellular urethra bioscaffolds for future tissue engineering applications, using bioscaffolds or bioscaffold-derived soluble products. Cellular removal was evaluated by H&E, DAPI and DNA quantification. The presence of specific ECM proteins was assessed through immunofluorescence staining and colorimetric assay kits. Human skeletal muscle myoblasts, muscle progenitor cells and adipose-derived stromal vascular fractions were used to evaluate the recellularization of the acellular urethra bioscaffolds...
February 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28155919/obtaining-spontaneously-beating-cardiomyocyte-like-cells-from-adipose-derived-stromal-vascular-fractions-cultured-on-enzyme-crosslinked-gelatin-hydrogels
#11
Gang Yang, Zhenghua Xiao, Xiaomei Ren, Haiyan Long, Kunlong Ma, Hong Qian, Yingqiang Guo
Heart failure often develops after acute myocardial infarction because the injured myocardial tissue fails to recover or regenerate. Stem cell transplantation using adult cell sources, such as adipose-derived stromal vascular fraction (SVF), draws extensive attention. In this study, SVF cells were isolated from rat adipose tissue and cultivated on enzyme-crosslinked gelatin hydrogels. Morphological features of cell development and spontaneous beating behavior from these cells were observed and recorded. Cardiac phenotypes were characterized via immunofluorescence staining, and the expression of cardiac-specific genes was measured via RT-PCR...
February 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28143470/current-use-of-autologous-adipose-tissue-derived-stromal-vascular-fraction-cells-for-orthopedic-applications
#12
REVIEW
Jaewoo Pak, Jung Hun Lee, Kwang Seung Park, Moonhee Park, Lin-Woo Kang, Sang Hee Lee
Autologous adipose stromal vascular fractions (SVFs) containing adipose tissue-derived stem cells (ASCs) are currently being used in clinical settings for various orthopedic applications for human patients. Due to its potential capability of regenerating cartilage, bone, and tendons, autologous adipose SVFs are being tried in treating patients with osteoarthritis (OA), chondromalacia, meniscus tear, osteonecrosis of the femoral head, and tendon injuries. Here, we have reviewed available human clinical studies with regard to patient applications of autologous adipose SVF containing ASCs, specifically assessing effectiveness and safety in the field of orthopedic disorders...
January 31, 2017: Journal of Biomedical Science
https://www.readbyqxmd.com/read/28116724/isolation-of-mature-adipocytes-from-white-adipose-tissue-and-gene-expression-studies-by-real-time-quantitative-rt-pcr
#13
Aurelie Nguyen Dinh Cat, Ana M Briones
The study of adipose tissue and more specifically of adipocytes is considered pivotal for dissecting molecular mechanisms responsible for alterations in several organs and systems, including adipose tissue, not only in obesity but also in other diseases (hypertension, heart failure). Adipose tissue is a complex tissue composed of adipocytes and the stromal vascular fraction which includes a heterogeneous population of preadipocytes, blood cells, endothelial cells, and macrophages. In the present chapter, methods are detailed to generate purified mature adipocytes from white adipose tissue by using enzymatic digestion...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28100503/chronic-kidney-disease-promotes-chronic-inflammation-in-visceral-white-adipose-tissue
#14
Dong Mei Xiang, Xiu Zhen Song, Zhan Mei Zhou, Yang Liu, Xiao Yan Dai, Xiang Lan Huang, Fan Fan Hou, Qiu Gen Zhou
White adipose tissue plays an important role in the development of metabolic disturbance which is a common feature in patients with chronic kidney disease (CKD). The effect of CKD on white adipose tissue remains poorly appreciated. Here we evaluated the inflammatory potential of visceral white adipose tissue in a rat model of CKD. The results showed production of pro-inflammatory cytokines and infiltration of macrophage in the tissue were significantly increased in CKD rats than sham rats. Moreover, the primary adipocytes and stromal vascular fraction under the condition of CKD could trigger the inflammatory response in each other...
January 18, 2017: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/28053001/cancer-cachexia-differentially-regulates-visceral-adipose-tissue-turnover
#15
Felipe de Oliveira Franco, Magno Alves Lopes, Felipe Dos Santos Henriques, Rodrigo Xavier das Neves, Cesário Bianchi Filho, Miguel Luiz Batista
Cancer cachexia (CC) is a progressive metabolic syndrome that is marked by severe body weight loss. Metabolic disarrangement of fat tissues is a very early event in CC, followed by adipose tissue (AT) atrophy and remodelling. However, there is little information regarding the possible involvement of cellular turnover in this process. Thus, in this study, we evaluated the effect of CC on AT turnover and fibrosis of mesenteric (MEAT) and retroperitoneal (RPAT) adipose tissue depots as possible factors that contribute to AT atrophy...
2017: Journal of Endocrinology
https://www.readbyqxmd.com/read/28052909/naturally-adipose-stromal-cell-enriched-fat-graft-comparative-polychromatic-flow-cytometry-study-of-fat-harvested-by-barbed-or-blunt-multihole-cannula
#16
Alessio Caggiati, Antonia Germani, Anna Di Carlo, Giovanna Borsellino, Maurizio C Capogrossi, Mario Picozza
BACKGROUND: Fat grafts enriched with cells of the stromal vascular fraction (SVF), especially adipose-derived stromal cells (ASCs), exhibit significantly improved retention over non enriched, plain fat. Different types of liposuction cannulae may yield lipoaspirates with different subpopulations of cells. Moreover, preparation of adipose tissue for transplantation typically involves centrifugation, which creates a density gradient of fat. OBJECTIVES: The authors sought to determine whether liposuction with a barbed or smooth cannula altered the enrichment of the SVF, and specifically ASCs, in low-density (LD) and high-density (HD) fractions of centrifuged adipose tissue...
January 3, 2017: Aesthetic Surgery Journal
https://www.readbyqxmd.com/read/28040462/comparison-between-pediatric-and-adult-adipose-mesenchymal-stromal-cells
#17
Olivier Abbo, Marion Taurand, Paul Monsarrat, Isabelle Raymond, Emmanuelle Arnaud, Sandra De Barros, Françoise Auriol, Philippe Galinier, Louis Casteilla, Valerie Planat-Benard
BACKGROUND: Adipose-derived mesenchymalstromal cells (ASC) are currently tested in regenerative medicine to promote tissue reconstruction after injury. Regardingautologous purpose, the possible loss of therapeutic function and cell properties during aging have been questioned in adults. To date no reliable information is available concerning ASC from pediatric patients and a better knowledge is required for clinical applications. METHODS: Subcutaneous adipose tissue was collected from 27 donors (0-1 years old) and 50 donors (1-12 years old) and compared with adult ASC for in vitro characteristics...
December 28, 2016: Cytotherapy
https://www.readbyqxmd.com/read/28018432/adipose-derived-mesenchymal-stem-cells-viability-and-differentiating-features-for-orthopaedic-reparative-applications-banking-of-adipose-tissue
#18
Ilaria Roato, Daniela Alotto, Dimas Carolina Belisario, Stefania Casarin, Mara Fumagalli, Irene Cambieri, Raimondo Piana, Maurizio Stella, Riccardo Ferracini, Carlotta Castagnoli
Osteoarthritis is characterized by loss of articular cartilage also due to reduced chondrogenic activity of mesenchymal stem cells (MSCs) from patients. Adipose tissue is an attractive source of MSCs (ATD-MSCs), representing an effective tool for reparative medicine, particularly for treatment of osteoarthritis, due to their chondrogenic and osteogenic differentiation capability. The treatment of symptomatic knee arthritis with ATD-MSCs proved effective with a single infusion, but multiple infusions could be also more efficacious...
2016: Stem Cells International
https://www.readbyqxmd.com/read/28017585/evaluation-of-the-stromal-vascular-fraction-of-adipose-tissue%C3%A2-as-the-basis-for-a-stem-cell-based-tissue-engineered-vascular-graft
#19
Jeffrey T Krawiec, Han-Tsung Liao, LaiYee Lily Kwan, Antonio D'Amore, Justin S Weinbaum, J Peter Rubin, William R Wagner, David A Vorp
OBJECTIVE: One of the rate-limiting barriers within the field of vascular tissue engineering is the lengthy fabrication time associated with expanding appropriate cell types in culture. One particularly attractive cell type for this purpose is the adipose-derived mesenchymal stem cell (AD-MSC), which is abundant and easily harvested from liposuction procedures. Even this cell type has its drawbacks, however, including the required culture period for expansion, which could pose risks of cellular transformation or contamination...
December 22, 2016: Journal of Vascular Surgery
https://www.readbyqxmd.com/read/28002902/bone-marrow-concentrated-cells-and-stromal-vascular-fraction-cells-injections-for-osteoarthritis-treatment-a-systematic-review
#20
G Vadalà, R Papalia, L La Verde, F Russo, V Denaro, M A Rosa
The aim of this systematic review is to examine current clinical evidences supporting the intraarticular injection of bone marrow concentrate cells (BMC) and adipose-derived stromal vascular fraction cells (SVF) for the treatment of osteoarthritis (OA). The research was performed on PubMed (Medline), EMBASE and Cochrane Library considering the English literature. Only clinical trials have been included. The systematic research identified twelve clinical trials. Articles included in the study, were one of level II, four of level III, six of level IV and one level V...
October 2016: Journal of Biological Regulators and Homeostatic Agents
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