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Adipose stromal vascular fraction

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https://www.readbyqxmd.com/read/29202515/recent-progresses-in-breast-reconstruction-stem-cells-biomaterials-and-growth-factors
#1
Farshad Zarei, Hadis Daraee
Breast cancer is one of the most deadly tumors in women, and new procedures for post- surgical breast reconstruction have been developed which includes autologous stromal vascular fraction (SVF), platelet-derived growth factors, biomaterials and various stem cells. Adipose derived-stem cells (ASCs) has been reported to be one of the most widely used stem cell for breast reconstruction because of their ability to form new functional adipose tissue and vessels and in combination with stromal vascular fraction has been also reported efficient for breast reconstruction...
December 4, 2017: Drug Research
https://www.readbyqxmd.com/read/29198704/ischemic-flap-survival-improvement-by-composition-selective-fat-grafting-with-novel-adipose-tissue-derived-product-stromal-vascular-fraction-gel
#2
Pan Zhang, Jingwei Feng, Yunjun Liao, Junrong Cai, Tao Zhou, Mingliang Sun, Jianhua Gao, Kai Gao
BACKGROUND: Flap necrosis due to insufficient blood supply is a common postoperative complication in random pattern flaps. Stem cell therapies have emerged as promising biologics for tissue ischemia. A novel fat derived product, stromal vascular fraction gel (SVF-gel), can be prepared with lipoaspirate through simple mechanical processing, removing only the lipid content. SVF-gel enriches adipose-derived stem cells and potentially beneficial for flap necrosis. METHODS: Nude mice ischemic flaps were treated with human SVF-gel, stromal vascular fraction (SVF) cell suspension or saline (n = 10)...
November 30, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29197029/vasculogenic-and-angiogenic-potential-of-adipose-stromal-vascular-fraction-cell-populations-in-vitro
#3
Joseph S Zakhari, Jacob Zabonick, Brian Gettler, Stuart K Williams
Adipose-derived stromal vascular fraction (SVF) is a heterogeneous cell source that contains endothelial cells, pericytes, smooth muscle cells, stem cells, and other accessory immune and stromal cells. The SVF cell population has been shown to support vasculogenesis in vitro as well vascular maturation in vivo. Matrigel, an extracellular matrix (ECM) mixture has been utilized in vitro to evaluate tube formation of purified endothelial cell systems. We have developed an in vitro system that utilizes freshly isolated SVF and ECM molecules both in pure form (fibrin, laminin, collagen) as well as premixed form (Matrigel) to evaluate endothelial tip cell formation, endothelial stalk elongation, and early stages of branching and inosculation...
December 1, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/29188676/autologous-lipotransfer-versus-stromal-vascular-fraction-enriched-lipoinjection-for-diabetic-foot-wounds-healing-a-pilot-study
#4
G Vicenti, G Solarino, V Pesce, L Moretti, A Notarnicola, M Carrozzo, F Rifino, B Moretti
Chronic ulcers of the lower limbs represent a significant social and economic burden. Diabetes is a strong risk factor for development of chronic lesions. Adult stem cells and growth factors derived from the adipose tissue are among the most promising therapeutic strategies for hard to heal wounds. Fat grafts have been used for several decades to treat soft tissue deformities, but despite its excellent characteristics, the outcome was unpredictable, due to partial necrosis and resorption of the graft. Stem cells’ enrichment of these grafts or their injection into the edges of the ulcers have shown encouraging results in various experimental settings...
October 2017: Journal of Biological Regulators and Homeostatic Agents
https://www.readbyqxmd.com/read/29157877/-treatment-of-wrinkles-of-the-upper-lip-by-emulsified-fat-or-nanofat-biological-and-clinical-study-about-4%C3%A2-cases
#5
F Mesguich Batel, B Bertrand, J Magalon, P François, M Velier, J Veran, S Mallet, E Jouve, F Sabatier, D Casanova
OBJECTIVE: Emulsified fat injection showed its interest in aesthetic facial surgery. The adipose tissue harvested is mechanically emulsified and filtered. The suspension obtained is injected into the dermis through small diameter needles (27 to 30 gauges). The objective of our study was to evaluate the biological composition of emulsified fat and its clinical effectiveness in the treatment of peri-oral wrinkles in 4 patients aged 50 to 59 years. MATERIAL AND METHOD: Each patient received an intradermal injection of emulsified fat in the peri-oral wrinkles prepared from abdominal fat under local anesthesia...
November 17, 2017: Annales de Chirurgie Plastique et Esthétique
https://www.readbyqxmd.com/read/29156823/differential-activation-of-fyn-kinase-distinguishes-saturated-and-unsaturated-fats-in-mouse-macrophages
#6
Elena Tarabra, Ting-Wen An Lee, Victor A Zammit, Manu Vatish, Eijiro Yamada, Jeffrey E Pessin, Claire C Bastie
Diet-induced obesity is associated with increased adipose tissue activated macrophages. Yet, how macrophages integrate fatty acid (FA) signals remains unclear. We previously demonstrated that Fyn deficiency (fynKO) protects against high fat diet-induced adipose tissue macrophage accumulation. Herein, we show that inflammatory markers and reactive oxygen species are not induced in fynKO bone marrow-derived macrophages exposed to the saturated FA palmitate, suggesting that Fyn regulates macrophage function in response to FA signals...
October 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/29131029/rapid-induction-of-osteogenic-markers-in-mesenchymal-stem-cells-by-adipose-derived-stromal-vascular-fraction-cells
#7
Jung-Won Choi, Sunhye Shin, Chang Youn Lee, Jiyun Lee, Hyang-Hee Seo, Soyeon Lim, Seahyoung Lee, Il-Kwon Kim, Hoon-Bum Lee, Sang Woo Kim, Ki-Chul Hwang
BACKGROUND/AIMS: Stromal vascular fraction (SVF) cells are a mixed cell population, and their regenerative capacity has been validated in various therapeutic models. The purpose of this study was to investigate the regenerative mechanisms utilized by implanted SVF cells. Using an in vitro co-culture system, we sought to determine whether SVF implantation into impaired tissue affects endogenous mesenchymal stem cell (MSC) differentiation; MSCs can differentiate into a variety of cell types, and they have a strong regenerative capacity despite their low numbers in impaired tissue...
November 3, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29124400/characterization-of-m1-and-m2-polarization-of-macrophages-in-vascularized-human-dermo-epidermal-skin-substitutes-in-vivo
#8
Agnes S Klar, Katarzyna Michalak-Mićka, Thomas Biedermann, Claudia Simmen-Meuli, Ernst Reichmann, Martin Meuli
AIMS AND OBJECTIVES: Vascularized bio-engineered human dermo-epidermal skin substitutes (vascDESS) hold promise for treating burn patients, including those with severe full-thickness wounds. We have previously shown that vascDESS promote wound healing by enhanced influx of macrophages and granulocytes. Immediately following transplantation, macrophages infiltrate the graft and differentiate into a pro-inflammatory (M1) or a pro-healing M2 phenotype. The aim of this study was to characterize the activation state of macrophages infiltrating skin transplants at distinct time points following transplantation...
November 9, 2017: Pediatric Surgery International
https://www.readbyqxmd.com/read/29113467/adipose-derived-mesenchymal-stem-cells-from-the-elderly-exhibit-decreased-migration-and-differentiation-abilities-with-senescent-properties
#9
Meichen Liu, Hua Lei, Ping Dong, Xin Fu, Zhigang Yang, Ying Yang, Jiguang Ma, Xia Liu, Yilin Cao, Ran Xiao
Adipose-derived stem cells (ASCs) can be applied extensively in the clinic because they can be easily isolated and cause less donor-site morbidity; however, their application can be complicated by patient-specific factors, such as age and harvest site. In this study, we systematically evaluated the effects of age on the quantity and quality of human adipose-derived mesenchymal stem cells (hASCs) isolated from excised chest subcutaneous adipose tissue and investigated the underlying molecular mechanism. hASCs were isolated from donors of 3 different age-groups (i...
September 2017: Cell Transplantation
https://www.readbyqxmd.com/read/29102952/comparison-of-the-viability-and-yield-of-adipose-derived-stem-cells-ascs-from-different-donor-areas
#10
Anastasios Tsekouras, Dimitrios Mantas, Diamantis I Tsilimigras, Demetrios Moris, Michael Kontos, George C Zografos
BACKGROUND: Adipose tissue contains variable amounts of stem cells that have recently attracted increased interest due to their strong proliferative and differentiation capacity. However, significant heterogeneity exists in terms of stem cell yield and viability profile among individuals as well as different donor areas. MATERIALS AND METHODS: Between June 2016 and July 2016, forty (40) women underwent outpatient cosmetic liposuction procedures conferring a total of 53 lipoaspirates; inner thigh (n=13), outer thigh (n=9), abdomen (n=9), waist (n=16) and inner knee (n=6)...
November 2017: In Vivo
https://www.readbyqxmd.com/read/29079730/engineering-of-an-angiogenic-niche-by-perfusion-culture-of-adipose-derived-stromal-vascular-fraction-cells
#11
Giulia Cerino, Emanuele Gaudiello, Manuele Giuseppe Muraro, Friedrich Eckstein, Ivan Martin, Arnaud Scherberich, Anna Marsano
In vitro recapitulation of an organotypic stromal environment, enabling efficient angiogenesis, is crucial to investigate and possibly improve vascularization in regenerative medicine. Our study aims at engineering the complexity of a vascular milieu including multiple cell-types, a stromal extracellular matrix (ECM), and molecular signals. For this purpose, the human adipose stromal vascular fraction (SVF), composed of a heterogeneous mix of pericytes, endothelial/stromal progenitor cells, was cultured under direct perfusion flow on three-dimensional (3D) collagen scaffolds...
October 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29077919/glucocorticoids-suppressing-the-browning-of-adipose-tissue-via-mir-19b-in-male-mice
#12
Yifan Lv, Jing Yu, Yunlu Sheng, Min Huang, Xiaocen Kong, Wenjuan Di, Juan Liu, Hong Zhou, Hui Liang, Guoxian Ding
Physiological levels of glucocorticoids are required for proper metabolic control, and excessive glucocorticoid action has been linked to a variety of pandemic metabolic diseases. microRNA (miRNA)-19b plays a critical role in the pathogenesis of glucocorticoid-induced metabolic diseases. This study sought to explore the potential of miRNA-based therapeutics targeting adipose tissue. Our results showed that overexpressed miR-19b in stromal vascular fraction cells derived from subcutaneous adipose tissue had the same effects as dexamethasone treatment on the inhibition of adipose browning and oxygen consumption rate...
October 24, 2017: Endocrinology
https://www.readbyqxmd.com/read/29067487/epigenetic-modifications-of-the-zfp-znf423-gene-control-murine-adipogenic-commitment-and-are-dysregulated-in-human-hypertrophic-obesity
#13
Michele Longo, Gregory A Raciti, Federica Zatterale, Luca Parrillo, Antonella Desiderio, Rosa Spinelli, Ann Hammarstedt, Shahram Hedjazifar, Jenny M Hoffmann, Cecilia Nigro, Paola Mirra, Francesca Fiory, Pietro Formisano, Claudia Miele, Ulf Smith, Francesco Beguinot
AIMS/HYPOTHESIS: Subcutaneous adipocyte hypertrophy is associated with insulin resistance and increased risk of type 2 diabetes, and predicts its future development independent of obesity. In humans, subcutaneous adipose tissue hypertrophy is a consequence of impaired adipocyte precursor cell recruitment into the adipogenic pathway rather than a lack of precursor cells. The zinc finger transcription factor known as zinc finger protein (ZFP) 423 has been identified as a major determinant of pre-adipocyte commitment and maintained white adipose cell function...
October 24, 2017: Diabetologia
https://www.readbyqxmd.com/read/29059354/inflammation-and-fibrosis-in-perirenal-adipose-tissue-of-patients-with-aldosterone-producing-adenoma
#14
Chunyan Wu, Huijian Zhang, Jiajun Zhang, Cuihua Xie, Cunxia Fan, Hongbin Zhang, Peng Wu, Qiang Wei, Wanlong Tan, Lingling Xu, Ling Wang, Yaoming Xue, Meiping Guan
The prevalence of primary aldosteronism is much higher than previously thought. Recent studies have shown that primary aldosteronism is related to a higher risk of cardiovascular events. However, the underlying mechanism is not yet clear. Here we investigate the characteristics, including inflammation, fibrosis and adipokine expression, of adipose tissues from different deposits in patients with aldosterone-producing adenoma (APA). Inflammation and fibrosis changes were evaluated in perirenal and subcutaneous adipose tissues obtained from patients with APA (n=16), normotension (NT; n=10) and essential hypertension (EH; n=5) undergoing laparoscopic surgery...
October 19, 2017: Endocrinology
https://www.readbyqxmd.com/read/29058626/combined-platelet-rich-plasma-and-lipofilling-treatment-provides-great-improvement-in-facial-skin-induced-lesion-regeneration-for-scleroderma-patients
#15
Francesco Virzì, Paola Bianca, Alessandro Giammona, Tiziana Apuzzo, Simone Di Franco, Laura Rosa Mangiapane, Maria Luisa Colorito, Dario Catalano, Emanuela Scavo, Annalisa Nicotra, Antonina Benfante, Giuseppe Pistone, Valentina Caputo, Francesco Dieli, Roberto Pirrello, Giorgio Stassi
BACKGROUND: The use of stem cells, including mesenchymal stem cells (MSCs), for regenerative medicine is gaining interest for the clinical benefits so far obtained in patients. This study investigates the use of adipose autologous tissue in combination with platelet-rich plasma (PRP) to improve the clinical outcome of patients affected by systemic sclerosis (SSc). METHODS: Adipose-derived mesenchymal stem cells (AD-MSCs) and PRPs were purified from healthy donors and SSc patients...
October 23, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29040421/effect-of-use-of-platelet-rich-plasma-prp-in-skin-with-intrinsic-aging-process
#16
Luiz Charles-de-Sá, Natale Ferreira Gontijo-de-Amorim, Christina Maeda Takiya, Radovan Borojevic, Donatella Benati, Paolo Bernardi, Andrea Sbarbati, Gino Rigotti
Background: In previous papers, we demonstrated that the treatment of human photoaged skin with stromal-vascular fraction-enriched fat or expanded adipose-derived stem cells showed a decrease of elastosis and the appearance of new oxytalan elastic fibers in dermis and an increase in the vascular network. The utilization of fat plus platelet-rich plasma (PRP) led to an increase in the vascular permeability and reactivity of the nervous component. Objectives: The purpose of this study was to analyze the histologic and ultrastructural changes of human skin after the injection of only PRP in the retroauricular area that was not exposed to sun and did not present the photoaging process, in comparison with our previous results...
September 7, 2017: Aesthetic Surgery Journal
https://www.readbyqxmd.com/read/29038672/safety-analysis-of-autologous-stem-cell-therapy-in-a-variety-of-degenerative-diseases-and-injuries-using-the-stromal-vascular-fraction
#17
Kristin Comella, Michelle Parlo, Rosemary Daly, Vincent Depasquale, Eric Edgerton, Patrick Mallory, Roy Schmidt, Walter P Drake
BACKGROUND: Stem cells from adipose tissue offer a novel therapy for patients with damaged tissue. Stromal vascular fraction (SVF) injected into patients may reduce inflammation, promote healing, and repair damaged/scarred tissue. SVF can be isolated from fat (adipose) tissue in an outpatient procedure. The SVF population includes mesenchymal stem cells (MSCs), pericytes, endothelial/progenitor cells, fibroblasts and growth factors where the adipocyte (fat cell) population has been removed...
November 2017: Journal of Clinical Medicine Research
https://www.readbyqxmd.com/read/29032969/the-effect-of-adipose-derived-stromal-vascular-fraction-on-stasis-zone-in-an-experimental-burn-model
#18
Atilla Adnan Eyuboglu, Cagri A Uysal, Gonca Ozgun, Erhan Coskun, Nilgun Markal Ertas, Mehmet Haberal
BACKGROUND: Stasis zone is the surrounding area of the coagulation zone which is an important part determining the extent of the necrosis in burn patients. In our study we aim to salvage the stasis zone by injecting adipose derived stromal vascular fraction (ADSVF). METHODS: Thermal injury was applied on dorsum of Sprague-Dawley rats (n=20) by the "comb burn" model as described previously. When the burn injury was established on Sprague-Dawley rats (30min); rat dorsum was separated into 2 equal parts consisting of 4 burn zones (3 stasis zone) on each pair...
October 9, 2017: Burns: Journal of the International Society for Burn Injuries
https://www.readbyqxmd.com/read/29027585/the-features-of-adipose-derived-stem-cells-in-patients-with-inflammatory-bowel-diseases
#19
Tsunekazu Mizushima, Tadafumi Fukata, Hiroshi Takeyama, Hidekazu Takahashi, Naotsugu Haraguchi, Junichi Nishimura, Taishi Hata, Chu Matsuda, Hirofumi Yamamoto, Yuichiro Doki, Masaki Mori
PURPOSE: The treatment outcomes for inflammatory bowel disease (IBD) have been improving, but the development of better therapies is needed. Stem cell therapy is promising, but little is known about the differences in adipose-derived stem cells (ADSCs) between IBD patients and healthy individuals. METHODS: ADSCs were isolated from subcutaneous adipose tissue (SAT) in IBD (Crohn's disease, 3; ulcerative colitis, 2) and non-IBD (colorectal cancer, 5; breast cancer, 1) patients...
October 12, 2017: Surgery Today
https://www.readbyqxmd.com/read/29025229/mechanical-supplementation-with-the-stromal-vascular-fraction-yields-improved-volume-retention-in-facial-lipotransfer-a-1-year-comparative-study
#20
Natale Ferreira Gontijo-de-Amorim, Luiz Charles-de-Sá, Gino Rigotti
Background: Unpredictable volume maintenance in the long term is a major limitation of autologous fat grafting. Objectives: The authors compared results of autologous lipotransfer to the face with or without enrichment of fat with the stromal vascular fraction (SVF). Methods: Thirty patients with asymmetric depletion of facial volume were included in a prospective study. Patients were randomly assigned to undergo a single session of autologous fat transfer with washed adipose tissue (control group) or with washed adipose tissue combined with the pellet of centrifuged lipoaspirate, which contained the SVF (enriched group)...
October 1, 2017: Aesthetic Surgery Journal
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