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Adipose stromal vascular fraction

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https://www.readbyqxmd.com/read/28690531/stem-cells-in-the-treatment-of-refractory-chronic-migraines
#1
Alexander Mauskop, Kenneth O Rothaus
BACKGROUND: Autologous adipose-derived stromal vascular fraction (SVF), which is rich in mesenchymal stromal cells, has been reported to be effective for the treatment of trigeminal neuropathic pain and chronic migraine and tension-type headaches. It is possible that stem cell activity targets neurogenic inflammation, which is a well-documented aspect of migraine pathogenesis. METHODS: Adult patients with severe migraine-related disability as measured by the Migraine Disability Assessment (MIDAS) score who failed botulinum toxin injections and at least 3 prophylactic drugs were included in this study...
May 2017: Case Reports in Neurology
https://www.readbyqxmd.com/read/28689790/cell-therapy-and-scarred-vocal-folds
#2
A Mattei, J Magalon, B Bertrand, C Philandrianos, J Veran, A Giovanni
Vocal fold microstructure is complex and can be affected by laryngeal microsurgery, inducing scarring that prevents mechanical uncoupling of epithelium and muscle, leading to vibration disorder and disabling dysphonia. Treatment options presently are few, and often without efficacy for vibration, having only an impact on volume to reduce glottal closure defect. The present review of the literature had two aims: (i) to report the current state of the literature on cell therapy in vocal fold scarring; and (ii) to analyze the therapeutic interest of the adipose-derived stromal vascular fraction in the existing therapeutic armamentarium...
July 6, 2017: European Annals of Otorhinolaryngology, Head and Neck Diseases
https://www.readbyqxmd.com/read/28687617/proinflammatory-cxcl12-cxcr4-cxcr7-signaling-axis-drives-myc-induced-prostate-cancer-in-obese-mice
#3
Achinto Saha, Songyeon Ahn, Jorge Blando, Fei Su, Mikhail G Kolonin, John DiGiovanni
Obesity is a prognostic risk factor in the progression of prostate cancer (PCa), however, the molecular mechanisms involved are unclear. In this study, we provide preclinical proof of concept for the role of a pro-inflammatory CXCL12-CXCR4/CXCR7 signaling axis in an obesity-driven mouse model of HiMyc prostate cancer. Analysis of the stromal vascular fraction (SVF) from periprostatic white adipose tissue (ppWAT) from obese HiMyc mice at 6 months of age revealed a dramatic increase in mRNAs encoding various chemokines, cytokines, growth factors and angiogenesis mediators, with CXCL12 among the most significantly upregulated genes...
July 7, 2017: Cancer Research
https://www.readbyqxmd.com/read/28683667/early-immunomodulatory-effects-of-implanted-human-perivascular-stromal-cells-during-bone-formation
#4
Carolyn A Meyers, Jiajia Xu, Lei Zhang, Greg Asatrian, Catherine Ding, Noah Yan, Kristen Broderick, Justin Sacks, Raghav Goyal, Xinli Zhang, Kang Ting, Bruno Péault, Chia Soo, Aaron W James
Human perivascular stem/stromal cells (PSC) are a multipotent mesodermal progenitor cell population defined by their perivascular residence. PSC are most commonly derived from subcutaneous adipose tissue, and recent studies have demonstrated the high potential for clinical translation of this fluorescence activated cell sorting (FACS) derived cell population for bone tissue engineering. Specifically, purified PSC induce greater bone formation than unpurified stroma taken from the same patient sample. Here, we examined the differences in early innate immune response to human PSC or unpurified stroma (stromal vascular fraction, SVF) during the in vivo process of bone formation...
July 6, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28676689/eicosapentaenoic-acid-and-5-hepe-enhance-macrophage-mediated-treg-induction-in-mice
#5
Toshiharu Onodera, Atsunori Fukuhara, Jihoon Shin, Tomonori Hayakawa, Michio Otsuki, Iichiro Shimomura
Eicosapentaenoic acid (EPA) is an omega-3 fatty acid with immunomodulatory and anti-inflammatory effects. Beyond its direct effects, the metabolic products of EPA also regulate various immune responses. Animal experiments demonstrated that EPA reduces adipose inflammation in high fat diet-induced obese mouse. However, the effects of EPA on infiltrated immune cell populations in adipose tissue and underlying mechanisms remain to be elucidated. We performed flow cytometry of stromal vascular fraction of epididymal adipose tissues from C57BL/6J and ob/ob mice fed normal chow mixed with or without 5% EPA...
July 4, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28673775/intramyocardial-bone-marrow-mononuclear-cells-versus-bone-marrow-derived-and-adipose-mesenchymal-cells-in-a-rat-model-of-dilated-cardiomyopathy
#6
M Dolores Carmona, Sagrario Cañadillas, Miguel Romero, Alfonso Blanco, Sonia Nogueras, Concha Herrera
BACKGROUND: Effects of cell therapy on dilated cardiomyopathy (DCM) have been investigated in pre-clinical models using distinct cellular types in each study. A single study that compares the effectiveness of different cells is lacking. METHODS: We have compared the effects of intramyocardial injection (IMI) of bone marrow (BM)-derived mononuclear cells (MNCs), BM and adipose tissue (AT) mesenchymal stromal cells (BM-MSCs and AT-MSCs) on heart function, histological changes and myocardial ultrastructure in a rat model of DCM...
June 30, 2017: Cytotherapy
https://www.readbyqxmd.com/read/28671889/cell-based-soft-tissue-reconstruction-in-a-hydrogel-scaffold
#7
Charles P Blackshear, John S Flacco, Stephanie M Vistnes, Natalie N Chung, Dre Irizarry, Elizabeth A Brett, Derek J Yen, Arash Momeni, Michael T Longaker, Derrick C Wan
BACKGROUND: Renevia is a hyaluronin-gelatin crosslinked matrix scaffold that has been studied as an alternative to adipose transfer in soft tissue reconstruction. It is designed to emulate the native extracellular matrix environment by supporting stromal vascular fraction (SVF) cell attachment, survival, and proliferation, thus promoting cell-based volume restoration. However, the concentration of incorporated cells for a clinically relevant result has yet to be determined. METHODS: Five experimental groups of seven CD-1 nude immunodeficient mice were given 250 μL grafts of the following composition: 1 million human SVF cells per mL of Renevia scaffold, 6 million human SVF cells per mL scaffold, 12 million human SVF cells per mL scaffold, Renevia scaffold-alone or human adipose tissue-alone...
June 1, 2017: Annals of Plastic Surgery
https://www.readbyqxmd.com/read/28670447/short-term-hypoxic-preconditioning-promotes-prevascularization-in-3d-bioprinted-bone-constructs-with-stromal-vascular-fraction-derived-cells
#8
Mitchell A Kuss, Robert Harms, Shaohua Wu, Ying Wang, Jason B Untrauer, Mark A Carlson, Bin Duan
Reconstruction of complex, craniofacial bone defects often requires autogenous vascularized bone grafts, and still remains a challenge today. In order to address this issue, we isolated the stromal vascular fraction (SVF) from adipose tissues and maintained the phenotypes and the growth of endothelial lineage cells within SVF derived cells (SVFC) by incorporating an endothelial cell medium. We 3D bioprinted SVFC within our hydrogel bioinks and conditioned the constructs in either normoxia or hypoxia. We found that short-term hypoxic conditioning promoted vascularization-related gene expression, whereas long-term hypoxia impaired cell viability and vascularization...
June 5, 2017: RSC Advances
https://www.readbyqxmd.com/read/28665236/formation-of-adipose-stromal-vascular-fraction-cell-laden-spheroids-using-a-3d-bioprinter-and-superhydrophobic-surfaces
#9
Brian Gettler, Joseph Zakhari, Piyani Gandhi, Stuart K Williams
The therapeutic infusion of adipose-derived stromal vascular fraction cells (SVF) for the treatment of multiple diseases, has progressed to numerous human clinical trials; however, the often poor retention of the cells following implantation remains a common drawback of direct cell injection. One solution to cellular retention at the injection site has been the use of biogels to encapsulate cells within a microenvironment prior to and upon implantation. The current study utilized 3D bioprinting technology to evaluate the ability to form SVF laden spheroids with collagen I as a gel forming biomatrix...
June 30, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28657470/point-of-care-adipose-derived-stromal-vascular-fraction-cell-isolation-and-eptfe-graft-sodding
#10
Stuart K Williams, Marvin E Morris, Paul Kosnik, Kevin D Lye, Gary Gentzkow, Charles B Ross, Amit J Dwivedi, Leigh B Kleinert
Adipose-derived stromal vascular fraction cell populations are being evaluated for numerous clinical applications. The current study evaluated a point of care (POC) technology, the Tissue Genesis "TGI 1000" Cell Isolation System™, to perform an automated isolation of adipose-derived stromal vascular fraction cells to be used in the fabrication of a tissue engineered vascular graft in the operating room. A total of 7 patients were enrolled in this FDA registered Investigational Device Exemption clinical trial and received femoral to tibial expanded polytetrafluoroethylene (ePTFE) bypass grafts to treat peripheral arterial disease...
June 28, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28651075/modulation-of-natriuretic-peptide-receptors-in-human-adipose-tissue-molecular-mechanisms-behind-the-natriuretic-handicap-in-morbidly-obese-patients
#11
Alessandra Gentili, Maria Rosaria Frangione, Elisa Albini, Carmine Vacca, Maria Anastasia Ricci, Stefano De Vuono, Marcello Boni, Fabio Rondelli, Luciana Rotelli, Graziana Lupattelli, Ciriana Orabona
The B-type natriuretic peptide (BNP) hormone plays a crucial role in the regulation of cardiovascular and energy homeostasis. Obesity is associated with low circulating levels of BNP, a condition known as "natriuretic handicap." Recent evidences suggest an altered expression of BNP receptors-both the signaling natriuretic peptide receptors (NPR)-A and the clearance NPR-C receptor-in adipose tissue (AT) as one of the putative causes of natriuretic handicap. The current study aims at clarifying the molecular mechanisms behind the natriuretic handicap, focusing on NPR modulation in the AT of obese and control subjects...
June 10, 2017: Translational Research: the Journal of Laboratory and Clinical Medicine
https://www.readbyqxmd.com/read/28646271/characterization-of-adipose-derived-stem-cells-following-burn-injury
#12
Anesh Prasai, Amina El Ayadi, Randy C Mifflin, Michael D Wetzel, Clark R Andersen, Heinz Redl, David N Herndon, Celeste C Finnerty
Severe burns induce a prolonged inflammatory response in subcutaneous adipose tissue that modulates signaling in adipose-derived stem cells (ASCs), which hold potential for healing burn wounds or generating skin substitutes. Using a 60% rat scald burn model, we conducted a series of experiments to determine which cells isolated from the adipose tissue produced inflammatory mediators and how these changes affect ASC fate and function. The stromal vascular fraction (SVF), adipocytes, and ASCs were isolated from adipose tissue at varying times up to 4 weeks postburn and from non-injured controls...
June 23, 2017: Stem Cell Reviews
https://www.readbyqxmd.com/read/28645702/use-of-adipose-tissue-and-stromal-vascular-fraction-in-hand-surgery
#13
REVIEW
Iad Nseir, Flore Delaunay, Charles Latrobe, Albane Bonmarchand, Dorothée Coquerel-Beghin, Isabelle Auquit-Auckbur
Adipose tissue is an abundant source of various cell types including not only adipocytes, but also progenitor and endothelial cells from thestroma. Interest in adipose tissue has surged since the identification in 2001 of adipose-derived stem cells (ADSCs) and of the stromal vascular fraction (SVF) obtained from adipose tissue by enzymatic digestion and centrifugation. SVF has been proven effective in ensuring tissue regeneration, thus improving tissue trophicityand vascularisation. These effects have generated strong interest among both physicians and surgeons, particularly in the field of hand surgery...
June 20, 2017: Orthopaedics & Traumatology, Surgery & Research: OTSR
https://www.readbyqxmd.com/read/28629476/stromal-vascular-fraction-cells-for-the-treatment-of-critical-limb-ischemia-a-pilot-study
#14
Adas Darinskas, Mindaugas Paskevicius, Gintaras Apanavicius, Gintaris Vilkevicius, Liutauras Labanauskas, Thomas E Ichim, Rytis Rimdeika
BACKGROUND: Cell-based therapy is being explored as an alternative treatment option for critical limb ischemia (CLI), a disease associated with high amputation and mortality rates and poor quality of life. However, therapeutic potential of uncultured adipose-derived stromal vascular fraction (SVF) cells has not been evaluated as a possible treatment. In this pilot study, we investigated the efficacy of multiple injections of autologous uncultured adipose-derived SVF cells to treat patients with CLI...
June 19, 2017: Journal of Translational Medicine
https://www.readbyqxmd.com/read/28629466/intra-articular-injection-in-the-knee-of-adipose-derived-stromal-cells-stromal-vascular-fraction-and-platelet-rich-plasma-for-osteoarthritis
#15
Himanshu Bansal, Kristin Comella, Jerry Leon, Poonam Verma, Diwaker Agrawal, Prasad Koka, Thomas Ichim
BACKGROUND: Stromal vascular fraction (SVF) can easily be obtained from a mini-lipoaspirate procedure of fat tissue and platelet rich plasma (PRP) can be obtained from peripheral blood. We evaluated the safety and preliminary efficacy of administering SVF and PRP intra-articularly into patients with osteoarthritis grade 1 and 2. METHODS: A total of ten patients underwent a local tumescent liposuction procedure to remove approximately 100 ml of fat tissue from the abdomen...
June 19, 2017: Journal of Translational Medicine
https://www.readbyqxmd.com/read/28619097/adipose-tissue-derived-stromal-vascular-fraction-in-regenerative-medicine-a-brief-review-on-biology-and-translation
#16
REVIEW
Pablo Bora, Anish S Majumdar
Adipose/fat tissue provides an abundant source of stromal vascular fraction (SVF) cells for immediate administration and can also give rise to a substantial number of cultured, multipotent adipose-derived stromal cells (ADSCs). Recently, both SVF and ADSCs have gained wide-ranging translational significance in regenerative medicine. Initially used for cosmetic breast enhancement, this mode of treatment has found use in many diseases involving immune disorders, tissue degeneration, and ischaemic conditions. In this review, we try to address several important aspects of this field, outlining the biology, technology, translation, and challenges related to SVF- and ADSC-based therapies...
June 15, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28612196/cell-source-differentiation-functional-stimulation-and-potential-application-of-human-thermogenic-adipocytes-in-vitro
#17
REVIEW
Dinh-Toi Chu, Yang Tao, Le Hoang Son, Duc-Hau Le
Recent investigations have showed that the functional thermogenic adipocytes are present in both infants and adult humans. Accumulating evidence suggests that the coexistence of classical and inducible brown (brite) adipocytes in humans at adulthood and these adipocytes function to generate heat from energy resulting in reducing body fat and improving glucose metabolism. Human thermogenic adipocytes can be differentiated in vitro from stem cells, cell lines, or adipose stromal vascular fraction. Pre-activated human brite adipocytes in vitro can maintain their thermogenic function in normal or obese immunodeficient mice; therefore, they improve glucose homeostasis and reduce fat mass in obese animals...
June 14, 2017: Journal of Physiology and Biochemistry
https://www.readbyqxmd.com/read/28593712/human-adipose-tissue-derived-tenomodulin-positive-subpopulation-of-stem-cells-a-promising-source-of-tendon-progenitor-cells
#18
A I Gonçalves, P M Gershovich, M T Rodrigues, R L Reis, M E Gomes
Cell-based therapies are of particular interest for tendon and ligament regeneration given the low regenerative potential of these tissues. Adipose tissue is an abundant source of stem cells, which may be employed for the healing of tendon lesions. However, human adult multipotent adipose-derived stem cells (hASCs) isolated from the stromal vascular fraction (SVF) of adipose tissue originate highly heterogeneous cell populations that hinder their use in specific tissue-oriented applications. In this study, distinct subpopulations of hASCs were immunomagnetic separated and their tenogenic differentiation capacity evaluated in the presence of several growth factors (GFs), namely EGF, bFGF, TGF-β1 and PDGF-BB, that are well-known regulators of tendon development, growth and healing...
June 7, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28567229/platelet-derived-growth-factor-receptor-positive-pericytic-cells-of-white-adipose-tissue-from-critical-limb-ischemia-patients-display-mesenchymal-stem-cell-like-properties
#19
Eo Jin Kim, Sang Gyo Seo, Hyuk Soo Shin, Doo Jae Lee, Ji Hye Kim, Dong Yeon Lee
BACKGROUND: The pericytes in the blood vessel wall have recently been identified to be important in regulating vascular formation, stabilization, remodeling, and function. We isolated and identified pericyte-like platelet-derived growth factor receptor beta-positive (PDGFRβ+) cells from the stromal vascular fraction (SVF) of adipose tissue from critical limb ischemia (CLI) patients and investigated their potential as a reliable source of stem cells for cell-based therapy. METHODS: De-identified subcutaneous fat tissues were harvested after amputation in CLI patients...
June 2017: Clinics in Orthopedic Surgery
https://www.readbyqxmd.com/read/28545230/nanog-plays-a-hierarchical-role-in-the-transcription-network-regulating-the-pluripotency-and-plasticity-of-adipose-tissue-derived-stem-cells
#20
Maria Pitrone, Giuseppe Pizzolanti, Laura Tomasello, Antonina Coppola, Lorenzo Morini, Gianni Pantuso, Romina Ficarella, Valentina Guarnotta, Sebastio Perrini, Francesco Giorgino, Carla Giordano
The stromal vascular cell fraction (SVF) of visceral and subcutaneous adipose tissue (VAT and SAT) has increasingly come into focus in stem cell research, since these compartments represent a rich source of multipotent adipose-derived stem cells (ASCs). ASCs exhibit a self-renewal potential and differentiation capacity. Our aim was to study the different expression of the embryonic stem cell markers NANOG (homeobox protein NANOG), SOX2 (SRY (sex determining region Y)-box 2) and OCT4 (octamer-binding transcription factor 4) and to evaluate if there exists a hierarchal role in this network in ASCs derived from both SAT and VAT...
May 23, 2017: International Journal of Molecular Sciences
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