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Adipose stromal vascular fraction

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https://www.readbyqxmd.com/read/27913088/effect-of-suction-pressures-on-cell-yield-and-functionality-of-the-adipose-derived-stromal-vascular-fraction
#1
Yuan-Wen Chen, Jing-Ru Wang, Xuan Liao, Sheng-Hong Li, Li-Ling Xiao, Biao Cheng, Guang-Hui Xie, Jian-Xing Song, Hong-Wei Liu
OBJECTIVE: The present study was performed to explore the effect of suction pressures on the cell yield of adipose-derived stromal vascular fraction (SVF) and the functionality of adipose-derived stem cells (ADSCs), situated in the SVF, and to develop optimal parameters of harvesting SVF for clinical use. METHODS: Adipose tissue was harvested from the lower abdomen of 10 patients by suction-assisted lipoplasty. Suction pressure was either -30 ± 5 kPa or -55 ± 5 kPa...
November 11, 2016: Journal of Plastic, Reconstructive & Aesthetic Surgery: JPRAS
https://www.readbyqxmd.com/read/27902740/extensive-characterization-and-comparison-of-endothelial-cells-derived-from-dermis-and-adipose-tissue-potential-use-in-tissue-engineering
#2
Hanneke N Monsuur, Ester M Weijers, Frank B Niessen, Amit Gefen, Pieter Koolwijk, Susan Gibbs, Lenie J van den Broek
Tissue-engineered constructs need to become quickly vascularized in order to ensure graft take. One way of achieving this is to incorporate endothelial cells (EC) into the construct. The adipose tissue stromal vascular fraction (adipose-SVF) might provide an alternative source for endothelial cells as adipose tissue can easily be obtained by liposuction. Since adipose-EC are now gaining more interest in tissue engineering, we aimed to extensively characterize endothelial cells from adipose tissue (adipose-EC) and compare them with endothelial cells from dermis (dermal-EC)...
2016: PloS One
https://www.readbyqxmd.com/read/27852267/culture-expansion-of-adipose-derived-stromal-cells-a-closed-automated-quantum-cell-expansion-system-compared-with-manual-flask-based-culture
#3
Mandana Haack-Sørensen, Bjarke Follin, Morten Juhl, Sonja K Brorsen, Rebekka H Søndergaard, Jens Kastrup, Annette Ekblond
BACKGROUND: Adipose derived stromal cells (ASCs) are a rich and convenient source of cells for clinical regenerative therapeutic approaches. However, applications of ASCs often require cell expansion to reach the needed dose. In this study, cultivation of ASCs from stromal vascular fraction (SVF) over two passages in the automated and functionally closed Quantum Cell Expansion System (Quantum system) is compared with traditional manual cultivation. METHODS: Stromal vascular fraction was isolated from abdominal fat, suspended in α-MEM supplemented with 10% Fetal Bovine Serum and seeded into either T75 flasks or a Quantum system that had been coated with cryoprecipitate...
November 16, 2016: Journal of Translational Medicine
https://www.readbyqxmd.com/read/27810224/the-utility-of-adipose-derived-stem-cells-and-stromal-vascular-fraction-for-oncologic-soft-tissue-reconstruction-is-it-safe-a-matter-for-debate
#4
Shaun S Tan, Weiliang Loh
Autologous free-fat grafting (AFG) has emerged as an attractive proposition for soft-tissue reconstruction of various contour defects because it obviates more complex reconstructive options and reduces operative times and donor-site morbidity. Nonetheless, a common complication of this procedure is the resorption of the engrafted fat. Cell-assisted lipotransfer (CAL) is now a well-regarded technique where adipose-derived stem cell (ASC)-rich stromal vascular fraction is admixed with lipoaspirate, increasing the volumetric outcome of fat grafts in light of its potent angiogenic and adipogenic properties...
November 1, 2016: Surgeon: Journal of the Royal Colleges of Surgeons of Edinburgh and Ireland
https://www.readbyqxmd.com/read/27777379/impaired-autophagy-activity-is-linked-to-elevated-er-stress-and-inflammation-in-aging-adipose-tissue
#5
Amiya Kumar Ghosh, Theresa Mau, Martin O'Brien, Sanjay Garg, Raymond Yung
Adipose tissue dysfunction in aging is associated with inflammation, metabolic syndrome and other diseases. We propose that impaired protein homeostasis due to compromised lysosomal degradation (micro-autophagy) might promote aberrant ER stress response and inflammation in aging adipose tissue. Using C57BL/6 mouse model, we demonstrate that adipose tissue-derived stromal vascular fraction (SVF) cells from old (18-20 months) mice have reduced expression of autophagy markers as compared to the younger (4-6 months) cohort...
October 24, 2016: Aging
https://www.readbyqxmd.com/read/27761084/role-of-adipose-derived-stem-cells-in-fat-grafting-and-reconstructive-surgery
#6
Shaun S Tan, Zhi Yang Ng, Weiqing Zhan, Warren Rozen
Autologous fat grafting is commonly utilised to reconstruct soft tissue defects caused by ageing, trauma, chronic wounds and cancer resection. The benefits of fat grafting are minimal donor site morbidity and ease of availability through liposuction or lipectomy. Nonetheless, survival and longevity of fat grafts remain poor post-engraftment. Various methods to enhance fat graft survival are currently under investigation and its stem cell constituents are of particular interest. Cell-assisted lipotransfer refers to the addition of adipose-derived stem cell (ASC) rich component of stromal vascular fraction to lipoaspirate, the results of which have proven promising...
July 2016: Journal of Cutaneous and Aesthetic Surgery
https://www.readbyqxmd.com/read/27757364/microcirculatory-response-in-vivo-on-local-intraarterial-infusion-of-autogenic-adipose-derived-stem-cells-or-stromal-vascular-fraction
#7
Wei Z Wang
: Both adipose-derived stem cells (ASCs) and stromal vascular fraction (SVF) have been demonstrated to have regenerative properties with therapeutic potential for numerous diseases through local or topical applications. However, it is unclear whether ASC or SVF can be delivered systemically through an intra-arterial infusion. The purpose of this study was to examine the microcirculatory response in vivo on local intraarterial infusion of autogenic ASCs or SVF in a vascular pedicle isolated rat cremaster microcirculation model...
September 2016: Plastic and Reconstructive Surgery. Global Open
https://www.readbyqxmd.com/read/27757339/shift-toward-mechanical-isolation-of-adipose-derived-stromal-vascular-fraction-review-of-upcoming-techniques
#8
Alexandra Condé-Green, Vasanth S Kotamarti, Lauren S Sherman, Jonathan D Keith, Edward S Lee, Mark S Granick, Pranela Rameshwar
: Standard isolation of adipose stromal vascular fraction (SVF) requires the use of collagenase and is considered more than "minimally manipulated" by current good manufacturing practice requirements. Alternatively, nonenzymatic isolation methods have surfaced using physical forces to separate cells from the adipose matrix. The purpose of this study was to review the literature on the use of mechanical isolation protocols and compare the results. The implication for use as a standard procedure in practice is discussed...
September 2016: Plastic and Reconstructive Surgery. Global Open
https://www.readbyqxmd.com/read/27748745/meteorin-like-metrnl-decreases-during-adipogenesis-and-inhibits-adipocyte-differentiation-leading-to-adipocyte-hypertrophy-in-humans
#9
D Löffler, K Landgraf, D Rockstroh, J T Schwartze, H Dunzendorfer, W Kiess, A Körner
BACKGROUND: Meteorin-like (METRNL) is a recently described circulating protein shown to be highly expressed in white adipose tissue and to beneficially affect energy metabolism in mice. OBJECTIVE: We systematically evaluated the role of METRNL for human adipogenesis and its association with obesity, browning and hyperinsulinemia in children. In addition, we assessed the functional relevance of METRNL for human adipogenesis. RESULTS: METRNL expression decreased during human adipocyte differentiation in vitro...
October 17, 2016: International Journal of Obesity: Journal of the International Association for the Study of Obesity
https://www.readbyqxmd.com/read/27738018/the-distribution-and-adipogenic-potential-of-perivascular-adipose-tissue-adipocyte-progenitors-is-dependent-on-sexual-dimorphism-and-vessel-location
#10
G Andres Contreras, Kyan Thelen, Nadia Ayala-Lopez, Stephanie W Watts
There are sex associated differences in the risk for cardiovascular comorbidities in obesity and metabolic syndrome. A common clinical finding in these diseases is the expansion of perivascular adipose tissues (PVAT) which is associated with alterations in their role as regulators of vessel function. PVAT hyperplasia and hypertrophy are dependent on the biology of populations of adipocyte progenitor cells (APC). It is currently unclear if PVAT enlargement diverges between males and females and the mechanisms linking APC biology with sexual dimorphism remain poorly understood...
October 2016: Physiological Reports
https://www.readbyqxmd.com/read/27737342/adipose-tissue-stromal-vascular-fraction-in-the-treatment-of-full-thickness-burns-in-rats
#11
Alexandre Lamaro Cardoso, Maria Márcia Bachion, Júlia de Miranda Morais, Marcelo Silva Fantinati, Vera Lúcia Lima de Almeida, Ruy Souza Lino
PURPOSE: To analyze the healing effects of stromal vascular fraction (SVF) application compared to wound dressing with 2% silver sulfadiazine in full thickness burn wounds in rats. METHODS: Animals were divided into two groups: 2% silver sulfadiazine group and SVF group. Both groups received occlusive bandages while the first one was treated with 2% silver sulfadiazine and the latter was treated with injections of SVF prepared from adipose tissue extracted from an animal donor...
September 2016: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/27733784/evaluation-of-adipose-derived-stromal-vascular-fraction-from-the-lateral-tailhead-inguinal-region-and-mesentery-of-horses
#12
Garrett L Metcalf, Scott R McClure, Jesse M Hostetter, Rudy F Martinez, Chong Wang
Use of mesenchymal stem cells (MSCs) found in the stromal vascular fraction (SVF) of equine adipose tissue has promising applications for regenerative therapies. The most commonly used source of equine adipose tissue is the subcutaneous tailhead. The objective of this study was to compare 3 adipose depot sites in horses and determine the viability and cellular yield, capillary density, gene expression for selected markers, and colony-forming unit fibroblasts (CFU-Fs) in adipose tissue taken from these sites...
October 2016: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
https://www.readbyqxmd.com/read/27733015/adipose-stromal-vascular-fraction-mediated-improvements-at-late-stage-disease-in-a-murine-model-of-multiple-sclerosis
#13
Annie C Bowles, Amy L Strong, Rachel M Wise, Robert C Thomas, Brittany Y Gerstein, Maria F Dutreil, Ryan S Hunter, Jeffrey M Gimble, Bruce A Bunnell
Multiple sclerosis (MS) is a common neurodegenerative disease and remains an unmet clinical challenge. In MS, an autoimmune response leads to immune cell infiltration, inflammation, demyelination, and lesions in central nervous system (CNS) tissues resulting in tremors, fatigue, and progressive loss of motor function. These pathologic hallmarks are effectively reproduced in the murine experimental autoimmune encephalomyelitis (EAE) model. The stromal vascular fraction (SVF) of adipose tissue is composed of adipose-derived stromal/stem cells (ASC), adipocytes, and various leukocytes...
October 12, 2016: Stem Cells
https://www.readbyqxmd.com/read/27729337/divergent-functions-of-endotrophin-on-different-cell-populations-in-adipose-tissue
#14
Yueshui Zhao, Xue Gu, Ningyan Zhang, Mikhail G Kolonin, Zhiqiang An, Kai Sun
Endotrophin is a cleavage product of collagen 6 (Col6) in adipose tissue (AT). Previously, we demonstrated that endotrophin serves as a costimulator to trigger fibrosis and inflammation within the unhealthy AT milieu. However, how endotrophin affects lipid storage and breakdown in AT and how different cell types in AT respond to endotrophin stimulation remain unknown. In the current study, by using a doxycycline (Dox)-inducible mouse model, we observed significant upregulation of adipogenic genes in the WAT of endotrophin Tg mice...
October 11, 2016: American Journal of Physiology. Endocrinology and Metabolism
https://www.readbyqxmd.com/read/27720147/adipose-tissue-remodeling-in-late-lactation-dairy-cows-during-feed-restriction-induced-negative-energy-balance
#15
G Andres Contreras, Kyan Thelen, Sarah E Schmidt, Clarissa Strieder-Barboza, Courtney L Preseault, William Raphael, Matti Kiupel, John Caron, Adam L Lock
Excessive rates of demand lipolysis in the adipose tissue (AT) during periods of negative energy balance (NEB) are associated with increased susceptibility to disease and limited lactation performance. Lipolysis induces a remodeling process within AT that is characterized by an inflammatory response, cellular proliferation, and changes in the extracellular matrix (ECMT). The adipose tissue macrophage (ATM) is a key component of the inflammatory response. Infiltration of ATM-forming cellular aggregates was demonstrated in transition cows, suggesting that ATM trafficking and phenotype changes may be associated with disease...
December 2016: Journal of Dairy Science
https://www.readbyqxmd.com/read/27717133/the-fractionation-of-adipose-tissue-procedure-to-obtain-stromal-vascular-fractions-for-regenerative-purposes
#16
Joris A van Dongen, Hieronymus P Stevens, Mojtaba Parvizi, Berend van der Lei, Martin C Harmsen
Autologous adipose tissue transplantation is clinically used to reduce dermal scarring and to restore volume loss. The therapeutic benefit on tissue damage more likely depends on the stromal vascular fraction of adipose tissue than on the adipocyte fraction. This stromal vascular fraction can be obtained by dissociation of adipose tissue, either enzymatically or mechanical. Enzymatic dissociation procedures are time-consuming and expensive. Therefore, we developed a new inexpensive mechanical dissociation procedure to obtain the stromal vascular fraction from adipose tissue in a time sparing way, which is directly available for therapeutic injection...
September 26, 2016: Wound Repair and Regeneration
https://www.readbyqxmd.com/read/27702746/caspase-1-deficiency-promotes-high-fat-diet-induced-adipose-tissue-inflammation-and-the-development-of-obesity
#17
Hiroaki Kimura, Tadayoshi Karasawa, Fumitake Usui, Akira Kawashima, Yuka Endo, Motoi Kobayashi, Ai Sadatomo, Jun Nakamura, Yusaku Iwasaki, Toshihiko Yada, Hiroko Tsutsui, Tadashi Kasahara, Masafumi Takahashi
Caspase-1 is a cysteine protease responsible for the processing of the proinflammatory cytokine interleukin-1β and activated by the formation of inflammasome complexes. Although several investigations have found a link between diet-induced obesity and caspase-1, the relationship of remains controversial. Here, we found that mice deficient in caspase-1 were susceptible to high-fat diet-induced obesity with increased adiposity as well as normal lipid and glucose metabolism. Caspase-1 deficiency clearly promoted the infiltration of inflammatory macrophages and increased the production of C-C motif chemokine ligand 2 (CCL2) in the adipose tissue...
October 4, 2016: American Journal of Physiology. Endocrinology and Metabolism
https://www.readbyqxmd.com/read/27702567/inhibition-of-m1-macrophage-activation-in-adipose-tissue-by-berberine-improves-insulin-resistance
#18
Lifang Ye, Shu Liang, Chao Guo, Xizhong Yu, Juan Zhao, Hao Zhang, Wenbin Shang
AIMS: Insulin resistance is associated with a chronic inflammation in adipose tissue which is propagated by a phenotypic switch in adipose tissue macrophage (ATM) polarization. This study aimed to investigate whether berberine, the major alkaloid of rhizoma coptidis, can improve insulin resistance through inhibiting ATM activation and inflammatory response in adipose tissue. MAIN METHODS: High-fat-diet induced obese mice were administered oral with berberine (50mg/kg/day) for 14days...
October 1, 2016: Life Sciences
https://www.readbyqxmd.com/read/27698672/adipose-derived-cells-stromal-vascular-fraction-transplanted-for-orthopedical-or-neurological-purposes-are-they-safe-enough
#19
Katarzyna Siennicka, Aleksandra Zolocinska, Karolina Stepien, Natalia Lubina-Dabrowska, Marzena Maciagowska, Ewa Zolocinska, Anna Slysz, Renata Piusinska-Macoch, Slawomir Mazur, Urszula Zdanowicz, Robert Smigielski, Adam Stepien, Zygmunt Pojda
Although mesenchymal stem cells are used in numerous clinical trials, the safety of their application is still a matter of concern. We have analysed the clinical results of the autologous adipose-derived stem cell treatment (stromal vascular fraction (SVF) containing adipose-derived stem cells, endothelial progenitors, and blood mononuclear cells) for orthopedic (cartilage, bone, tendon, or combined joint injuries) and neurologic (multiple sclerosis) diseases. Methods of adipose tissue collection, cell isolation and purification, and resulting cell numbers, viability, and morphology were considered, and patient's age, sex, disease type, and method of cell administration (cell numbers per single application, treatment numbers and frequency, and methods of cell implantation) were analysed and searched for the unwanted clinical effects...
2016: Stem Cells International
https://www.readbyqxmd.com/read/27641055/the-use-of-adipose-derived-stromal-vascular-fraction-cells-and-platelet-rich-plasma-in-regenerative-plastic-surgery
#20
Pietro Gentile, Maria Giovanna Scioli, Alessandra Bielli, Augusto Orlandi, Valerio Cervelli
Tissue engineering has emerged at the intersection of numerous disciplines to meet a global clinical need for technologies to promote the regeneration of tissues. Recently, many authors have focused their attention on mesenchymal stem/stromal cells (MSCs) for their capacity to differentiate into many cell lineages. The most widely studied cell types are bone marrow mesenchymal stem cells (BM-MSCs) and adipose-derived stem cells (ASCs), which display similar results. Biomaterials, cells, and growth factors are needed to design a regenerative plastic surgery approach in the treatment of organ and tissue defects, but not all tissues are created equal...
September 19, 2016: Stem Cells
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