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Adipose stromal vascular fraction

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https://www.readbyqxmd.com/read/28629476/stromal-vascular-fraction-cells-for-the-treatment-of-critical-limb-ischemia-a-pilot-study
#1
Adas Darinskas, Mindaugas Paskevicius, Gintaras Apanavicius, Gintaris Vilkevicius, Liutauras Labanauskas, Thomas E Ichim, Rytis Rimdeika
BACKGROUND: Cell-based therapy is being explored as an alternative treatment option for critical limb ischemia (CLI), a disease associated with high amputation and mortality rates and poor quality of life. However, therapeutic potential of uncultured adipose-derived stromal vascular fraction (SVF) cells has not been evaluated as a possible treatment. In this pilot study, we investigated the efficacy of multiple injections of autologous uncultured adipose-derived SVF cells to treat patients with CLI...
June 19, 2017: Journal of Translational Medicine
https://www.readbyqxmd.com/read/28629466/intra-articular-injection-in-the-knee-of-adipose-derived-stromal-cells-stromal-vascular-fraction-and-platelet-rich-plasma-for-osteoarthritis
#2
Himanshu Bansal, Kristin Comella, Jerry Leon, Poonam Verma, Diwaker Agrawal, Prasad Koka, Thomas Ichim
BACKGROUND: Stromal vascular fraction (SVF) can easily be obtained from a mini-lipoaspirate procedure of fat tissue and platelet rich plasma (PRP) can be obtained from peripheral blood. We evaluated the safety and preliminary efficacy of administering SVF and PRP intra-articularly into patients with osteoarthritis grade 1 and 2. METHODS: A total of ten patients underwent a local tumescent liposuction procedure to remove approximately 100 ml of fat tissue from the abdomen...
June 19, 2017: Journal of Translational Medicine
https://www.readbyqxmd.com/read/28619097/adipose-tissue-derived-stromal-vascular-fraction-in-regenerative-medicine-a-brief-review-on-biology-and-translation
#3
REVIEW
Pablo Bora, Anish S Majumdar
Adipose/fat tissue provides an abundant source of stromal vascular fraction (SVF) cells for immediate administration and can also give rise to a substantial number of cultured, multipotent adipose-derived stromal cells (ADSCs). Recently, both SVF and ADSCs have gained wide-ranging translational significance in regenerative medicine. Initially used for cosmetic breast enhancement, this mode of treatment has found use in many diseases involving immune disorders, tissue degeneration, and ischaemic conditions. In this review, we try to address several important aspects of this field, outlining the biology, technology, translation, and challenges related to SVF- and ADSC-based therapies...
June 15, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28612196/cell-source-differentiation-functional-stimulation-and-potential-application-of-human-thermogenic-adipocytes-in-vitro
#4
REVIEW
Dinh-Toi Chu, Yang Tao, Le Hoang Son, Duc-Hau Le
Recent investigations have showed that the functional thermogenic adipocytes are present in both infants and adult humans. Accumulating evidence suggests that the coexistence of classical and inducible brown (brite) adipocytes in humans at adulthood and these adipocytes function to generate heat from energy resulting in reducing body fat and improving glucose metabolism. Human thermogenic adipocytes can be differentiated in vitro from stem cells, cell lines, or adipose stromal vascular fraction. Pre-activated human brite adipocytes in vitro can maintain their thermogenic function in normal or obese immunodeficient mice; therefore, they improve glucose homeostasis and reduce fat mass in obese animals...
June 14, 2017: Journal of Physiology and Biochemistry
https://www.readbyqxmd.com/read/28593712/human-adipose-tissue-derived-tenomodulin-positive-subpopulation-of-stem-cells-a-promising-source-of-tendon-progenitor-cells
#5
A I Gonçalves, P M Gershovich, M T Rodrigues, R L Reis, M E Gomes
Cell-based therapies are of particular interest for tendon and ligament regeneration given the low regenerative potential of these tissues. Adipose tissue is an abundant source of stem cells, which may be employed for the healing of tendon lesions. However, human adult multipotent adipose-derived stem cells (hASCs) isolated from the stromal vascular fraction (SVF) of adipose tissue originate highly heterogeneous cell populations that hinder their use in specific tissue-oriented applications. In this study, distinct subpopulations of hASCs were immunomagnetic separated and their tenogenic differentiation capacity evaluated in the presence of several growth factors (GFs), namely EGF, bFGF, TGF-β1 and PDGF-BB, that are well-known regulators of tendon development, growth and healing...
June 7, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28567229/platelet-derived-growth-factor-receptor-positive-pericytic-cells-of-white-adipose-tissue-from-critical-limb-ischemia-patients-display-mesenchymal-stem-cell-like-properties
#6
Eo Jin Kim, Sang Gyo Seo, Hyuk Soo Shin, Doo Jae Lee, Ji Hye Kim, Dong Yeon Lee
BACKGROUND: The pericytes in the blood vessel wall have recently been identified to be important in regulating vascular formation, stabilization, remodeling, and function. We isolated and identified pericyte-like platelet-derived growth factor receptor beta-positive (PDGFRβ+) cells from the stromal vascular fraction (SVF) of adipose tissue from critical limb ischemia (CLI) patients and investigated their potential as a reliable source of stem cells for cell-based therapy. METHODS: De-identified subcutaneous fat tissues were harvested after amputation in CLI patients...
June 2017: Clinics in Orthopedic Surgery
https://www.readbyqxmd.com/read/28545230/nanog-plays-a-hierarchical-role-in-the-transcription-network-regulating-the-pluripotency-and-plasticity-of-adipose-tissue-derived-stem-cells
#7
Maria Pitrone, Giuseppe Pizzolanti, Laura Tomasello, Antonina Coppola, Lorenzo Morini, Gianni Pantuso, Romina Ficarella, Valentina Guarnotta, Sebastio Perrini, Francesco Giorgino, Carla Giordano
The stromal vascular cell fraction (SVF) of visceral and subcutaneous adipose tissue (VAT and SAT) has increasingly come into focus in stem cell research, since these compartments represent a rich source of multipotent adipose-derived stem cells (ASCs). ASCs exhibit a self-renewal potential and differentiation capacity. Our aim was to study the different expression of the embryonic stem cell markers NANOG (homeobox protein NANOG), SOX2 (SRY (sex determining region Y)-box 2) and OCT4 (octamer-binding transcription factor 4) and to evaluate if there exists a hierarchal role in this network in ASCs derived from both SAT and VAT...
May 23, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28503189/eicosapentaenoic-acid-shows-anti-inflammatory-effect-via-gpr120-in-3t3-l1-adipocytes-and-attenuates-adipose-tissue-inflammation-in-diet-induced-obese-mice
#8
Hodaka Yamada, Tomio Umemoto, Masafumi Kakei, Shin-Ichi Momomura, Masanobu Kawakami, San-E Ishikawa, Kazuo Hara
BACKGROUND: Saturated fatty acids have been shown to cause insulin resistance and low-grade chronic inflammation, whereas unsaturated fatty acids suppress inflammation via G-protein coupled receptor 120 (GPR120) in macrophages. However, the anti-inflammatory effects of unsaturated fatty acids in adipocytes have yet to be elucidated. Hence, the aims of the present study were to evaluate the anti-inflammatory effects of eicosapentaenoic acid (EPA) via GPR120 in adipocytes. METHODS: We used 250 μM palmitate as a representative saturated fatty acid...
2017: Nutrition & Metabolism
https://www.readbyqxmd.com/read/28498891/adipose-tissue-depots-and-inflammation-effects-on-plasticity-and-resident-mesenchymal-stem-cell-function
#9
Lina Badimon, Judit Cubedo
Adipose tissue (AT) is a highly heterogeneous organ. Beside the heterogeneity associated to different tissue types (white, brown and "brite") and its location-related heterogeneity (subcutaneous, visceral, epicardial, and perivascular, etc), AT composition, structure and functionality is highly dependent on individual-associated factors. As such, the pro-inflammatory state associated to the presence of obesity and other cardiovascular risk factors directly affects AT metabolism. Furthermore, the adipose-derived stem cells (ASCs) that reside in the stromal vascular fraction (SVF) of AT, besides being responsible for most of the plasticity attributed to AT, is an additional source of heterogeneity...
May 11, 2017: Cardiovascular Research
https://www.readbyqxmd.com/read/28486783/extracorporeal-shock-wave-therapy-in-situ-a-novel-approach-to-obtain-an-activated-fat-graft
#10
E Priglinger, M Sandhofer, A Peterbauer-Scherb, C Wurzer, C Steffenhagen, J Maier, W Holnthoner, S Nürnberger, H Redl, S Wolbank
One of the mainstays of facial rejuvenation strategies is volume restoration which can be achieved by autologous fat grafting. In our novel approach, we treated the adipose tissue harvest site with extracorporeal shock wave therapy (ESWT) in order to improve the quality of the regenerative cells in situ. The latter was demonstrated by characterizing the cells of the stromal vascular fraction (SVF) in the harvested liposuction material regarding cell yield, ATP content, proliferative capacity, surface marker profile, differentiation potential and secretory protein profile...
May 9, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28486770/melatonin-promotes-survival-of-non-vascularized-fat-grafts-and-enhances-the-viability-and-migration-of-human-adipose-derived-stem-cells-via-down-regulation-of-acute-inflammatory-cytokines
#11
Shaun S Tan, Weiqing Zhan, Christopher J Phoon, Xiaolian Han, Diego Marre, Sholeh Boodhun, Jason A Palmer, Geraldine M Mitchell, Wayne A Morrison
Non-vascularized fat grafting is a valuable technique for soft tissue reconstruction but poor survival of fat in the host environment remains a problem. A process known as cell-assisted transfer is used to enhance fat graft retention by adding stromal vascular fraction, an adipose-derived stem cell (ASC) rich content to lipoaspirate. We have recently shown that the use of melatonin, a ROS scavenger, protects human ASCs from hydrogen peroxide induced oxidative stress and cell death in vitro but its role as a pharmacologic adjunct in clinical fat grafting has not been studied...
May 9, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28485099/ectopic-bone-formation-by-aggregated-mesenchymal-stem-cells-from-bone-marrow-and-adipose-tissue-a-comparative-study
#12
Eelco M Fennema, Laurent A H Tchang, Huipin Yuan, Clemens A van Blitterswijk, Ivan Martin, Arnaud Scherberich, Jan de Boer
Tissue engineered constructs (TECs) based on spheroids of bone marrow mesenchymal stromal cells (BM-MSCs) combined with calcium phosphate (CaP) micro-particles and enveloped in a platelet-rich-plasma (PRP) hydrogel showed that aggregation of MSCs improves their ectopic bone formation potential. The stromal vascular fraction (SVF) and adipose-derived MSCs (ASCs) have been recognized as interesting MSC source for bone tissue engineering but their ectopic bone formation is limited. We investigated whether aggregation of ASCs could similarly improve ectopic bone formation by ASCs and SVF cells...
May 9, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28484594/adipose-tissue-lipolysis-and-remodeling-during-the-transition-period-of-dairy-cows
#13
REVIEW
G Andres Contreras, Clarissa Strieder-Barboza, William Raphael
Elevated concentrations of plasma fatty acids in transition dairy cows are significantly associated with increased disease susceptibility and poor lactation performance. The main source of plasma fatty acids throughout the transition period is lipolysis from adipose tissue depots. During this time, plasma fatty acids serve as a source of calories mitigating the negative energy balance prompted by copious milk synthesis and limited dry matter intake. Past research has demonstrated that lipolysis in the adipose organ is a complex process that includes not only the activation of lipolytic pathways in response to neural, hormonal, or paracrine stimuli, but also important changes in the structure and cellular distribution of the tissue in a process known as adipose tissue remodeling...
2017: Journal of Animal Science and Biotechnology
https://www.readbyqxmd.com/read/28482232/control-of-angiogenesis-and-host-response-by-modulating-the-cell-adhesion-properties-of-an-elastin-like-recombinamer-based-hydrogel
#14
Sebastian Manuel Staubli, Giulia Cerino, Israel Gonzalez De Torre, Matilde Alonso, Daniel Oertli, Friedrich Eckstein, Katharina Glatz, José Carlos Rodríguez Cabello, Anna Marsano
The control of the in vivo vascularization of engineered tissue substitutes is essential in order to obtain either a rapid induction or a complete inhibition of the process (e.g. in muscles and hyaline-cartilage, respectively). Among the several polymers available, Elastin-Like Recombinamers (ELR)-based hydrogel stands out as a promising material for tissue engineering thanks to its viscoelastic properties, non-toxicity, and non-immunogenicity. In this study, we hypothesized that varying the cell adhesion properties of ELR-hydrogels could modulate the high angiogenic potential of adipose tissue-derived stromal vascular fraction (SVF) cells, predominantly composed of endothelial/mural and mesenchymal cells...
August 2017: Biomaterials
https://www.readbyqxmd.com/read/28468789/intracoronary-administration-of-allogeneic-adipose-tissue-derived-mesenchymal-stem-cells-improves-myocardial-perfusion-but-not-left-ventricle-function-in-a-translational-model-of-acute-myocardial-infarction
#15
Joaquim Bobi, Núria Solanes, Rodrigo Fernández-Jiménez, Carlos Galán-Arriola, Ana Paula Dantas, Leticia Fernández-Friera, Carolina Gálvez-Montón, Elisabet Rigol-Monzó, Jaume Agüero, José Ramírez, Mercè Roqué, Antoni Bayés-Genís, Javier Sánchez-González, Ana García-Álvarez, Manel Sabaté, Santiago Roura, Borja Ibáñez, Montserrat Rigol
BACKGROUND: Autologous adipose tissue-derived mesenchymal stem cells (ATMSCs) therapy is a promising strategy to improve post-myocardial infarction outcomes. In a porcine model of acute myocardial infarction, we studied the long-term effects and the mechanisms involved in allogeneic ATMSCs administration on myocardial performance. METHODS AND RESULTS: Thirty-eight pigs underwent 50 minutes of coronary occlusion; the study was completed in 33 pigs. After reperfusion, allogeneic ATMSCs or culture medium (vehicle) were intracoronarily administered...
May 3, 2017: Journal of the American Heart Association
https://www.readbyqxmd.com/read/28464475/isolation-of-cd248-expressing-stromal-vascular-fraction-for-targeted-improvement-of-wound-healing
#16
Elizabeth Brett, Elizabeth R Zielins, Monica Chin, Michael Januszyk, Charles P Blackshear, Michael Findlay, Arash Momeni, Geoffrey C Gurtner, Michael T Longaker, Derrick C Wan
Wound healing remains a global issue of disability, cost, and health. Addition of cells from the stromal vascular fraction (SVF) of adipose tissue has been shown to increase the rate of full thickness wound closure. This study aimed to investigate the angiogenic mechanisms of CD248+ SVF cells in the context of full thickness excisional wounds. Single cell transcriptional analysis was used to identify and cluster angiogenic gene-expressing cells, which was then correlated with surface marker expression. SVF cells isolated from human lipoaspirate were FACS sorted based on the presence of CD248...
May 2, 2017: Wound Repair and Regeneration
https://www.readbyqxmd.com/read/28457726/cell-assisted-lipotransfer-current-concepts
#17
J Laloze, A Varin, N Bertheuil, J L Grolleau, C Vaysse, B Chaput
Autologous fat transfer, or lipofilling, is a common technique used for soft tissue reconstruction. It has been used for many years, but the technique is associated with a significant graft resorption rate (20% to 80%). To improve the fat graft survival rate, several methods have been tested, and one has appeared more promising: cell-assisted lipotransfer (CAL). In the CAL method, fat is enriched with adipose-derived stromal cells (ASC), contained in the stromal vascular fraction (SVF) obtained after enzymatic digestion of fat or after cell culture to improve the fat survival rate...
April 27, 2017: Annales de Chirurgie Plastique et Esthétique
https://www.readbyqxmd.com/read/28454682/the-adipose-tissue-derived-stromal-vascular-fraction-cells-from-lipedema-patients-are-they-different
#18
Eleni Priglinger, Christoph Wurzer, Carolin Steffenhagen, Julia Maier, Victoria Hofer, Anja Peterbauer, Sylvia Nuernberger, Heinz Redl, Susanne Wolbank, Matthias Sandhofer
BACKGROUND AIMS: Lipedema is a hormone-related disease of women characterized by enlargement of the extremities caused by subcutaneous deposition of adipose tissue. In healthy patients application of autologous adipose tissue-derived cells has shown great potential in several clinical studies for engrafting of soft tissue reconstruction in recent decades. The majority of these studies have used the stromal vascular fraction (SVF), a heterogeneous cell population containing adipose-derived stromal/stem cells (ASC), among others...
July 2017: Cytotherapy
https://www.readbyqxmd.com/read/28453382/flow-cytometric-single-cell-analysis-reveals-heterogeneity-between-adipose-depots
#19
Badwi B Boumelhem, Stephen J Assinder, Kim S Bell-Anderson, Stuart T Fraser
Understanding adipose tissue heterogeneity is hindered by the paucity of methods to analyze mature adipocytes at the single cell level. Here, we report a system for analyzing live adipocytes from different adipose depots in the adult mouse. Single cell suspensions of buoyant adipocytes were separated from the stromal vascular fraction and analyzed by flow cytometry. Compared to other lipophilic dyes, Nile Red uptake effectively distinguished adipocyte populations. Nile Red fluorescence increased with adipocyte size and granularity and could be combined with MitoTracker® Deep Red or fluorescent antibody labeling to further dissect adipose populations...
April 3, 2017: Adipocyte
https://www.readbyqxmd.com/read/28447575/adipose-derived-mesenchymal-stem-cells-from-the-elderly-exhibit-decreased-migration-and-differentiation-abilities-with-senescent-properties
#20
Meichen Liu, Hua Lei, Ping Dong, Xin Fu, Zhigang Yang, Ying Yang, Jiguang Ma, Xia Liu, Yilin Cao, Ran Xiao
Adipose-derived stem cells (ADSCs) can be applied extensively in the clinic because they can be easily isolated and cause less donor-site morbidity; however, their application can be complicated by patient-specific factors, such as age and harvest site. In this study, we systematically evaluated the effects of age on the quantity and quality of hADSCs isolated from excised chest subcutaneous adipose tissue and investigated the underlying molecular mechanism. hADSCs were isolated from donors of three different age groups (i...
April 26, 2017: Cell Transplantation
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