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Adipose stromal vascular fraction

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https://www.readbyqxmd.com/read/29040421/effect-of-use-of-platelet-rich-plasma-prp-in-skin-with-intrinsic-aging-process
#1
Luiz Charles-de-Sá, Natale Ferreira Gontijo-de-Amorim, Christina Maeda Takiya, Radovan Borojevic, Donatella Benati, Paolo Bernardi, Andrea Sbarbati, Gino Rigotti
Background: In previous papers, we demonstrated that the treatment of human photoaged skin with stromal-vascular fraction-enriched fat or expanded adipose-derived stem cells showed a decrease of elastosis and the appearance of new oxytalan elastic fibers in dermis and an increase in the vascular network. The utilization of fat plus platelet-rich plasma (PRP) led to an increase in the vascular permeability and reactivity of the nervous component. Objectives: The purpose of this study was to analyze the histologic and ultrastructural changes of human skin after the injection of only PRP in the retroauricular area that was not exposed to sun and did not present the photoaging process, in comparison with our previous results...
September 7, 2017: Aesthetic Surgery Journal
https://www.readbyqxmd.com/read/29038672/safety-analysis-of-autologous-stem-cell-therapy-in-a-variety-of-degenerative-diseases-and-injuries-using-the-stromal-vascular-fraction
#2
Kristin Comella, Michelle Parlo, Rosemary Daly, Vincent Depasquale, Eric Edgerton, Patrick Mallory, Roy Schmidt, Walter P Drake
BACKGROUND: Stem cells from adipose tissue offer a novel therapy for patients with damaged tissue. Stromal vascular fraction (SVF) injected into patients may reduce inflammation, promote healing, and repair damaged/scarred tissue. SVF can be isolated from fat (adipose) tissue in an outpatient procedure. The SVF population includes mesenchymal stem cells (MSCs), pericytes, endothelial/progenitor cells, fibroblasts and growth factors where the adipocyte (fat cell) population has been removed...
November 2017: Journal of Clinical Medicine Research
https://www.readbyqxmd.com/read/29032969/the-effect-of-adipose-derived-stromal-vascular-fraction-on-stasis-zone-in-an-experimental-burn-model
#3
Atilla Adnan Eyuboglu, Cagri A Uysal, Gonca Ozgun, Erhan Coskun, Nilgun Markal Ertas, Mehmet Haberal
BACKGROUND: Stasis zone is the surrounding area of the coagulation zone which is an important part determining the extent of the necrosis in burn patients. In our study we aim to salvage the stasis zone by injecting adipose derived stromal vascular fraction (ADSVF). METHODS: Thermal injury was applied on dorsum of Sprague-Dawley rats (n=20) by the "comb burn" model as described previously. When the burn injury was established on Sprague-Dawley rats (30min); rat dorsum was separated into 2 equal parts consisting of 4 burn zones (3 stasis zone) on each pair...
October 9, 2017: Burns: Journal of the International Society for Burn Injuries
https://www.readbyqxmd.com/read/29027585/the-features-of-adipose-derived-stem-cells-in-patients-with-inflammatory-bowel-diseases
#4
Tsunekazu Mizushima, Tadafumi Fukata, Hiroshi Takeyama, Hidekazu Takahashi, Naotsugu Haraguchi, Junichi Nishimura, Taishi Hata, Chu Matsuda, Hirofumi Yamamoto, Yuichiro Doki, Masaki Mori
PURPOSE: The treatment outcomes for inflammatory bowel disease (IBD) have been improving, but the development of better therapies is needed. Stem cell therapy is promising, but little is known about the differences in adipose-derived stem cells (ADSCs) between IBD patients and healthy individuals. METHODS: ADSCs were isolated from subcutaneous adipose tissue (SAT) in IBD (Crohn's disease, 3; ulcerative colitis, 2) and non-IBD (colorectal cancer, 5; breast cancer, 1) patients...
October 12, 2017: Surgery Today
https://www.readbyqxmd.com/read/29025229/mechanical-supplementation-with-the-stromal-vascular-fraction-yields-improved-volume-retention-in-facial-lipotransfer-a-1-year-comparative-study
#5
Natale Ferreira Gontijo-de-Amorim, Luiz Charles-de-Sá, Gino Rigotti
Background: Unpredictable volume maintenance in the long term is a major limitation of autologous fat grafting. Objectives: The authors compared results of autologous lipotransfer to the face with or without enrichment of fat with the stromal vascular fraction (SVF). Methods: Thirty patients with asymmetric depletion of facial volume were included in a prospective study. Patients were randomly assigned to undergo a single session of autologous fat transfer with washed adipose tissue (control group) or with washed adipose tissue combined with the pellet of centrifuged lipoaspirate, which contained the SVF (enriched group)...
October 1, 2017: Aesthetic Surgery Journal
https://www.readbyqxmd.com/read/29025216/assisted-salvage-of-ischemic-fasciocutaneous-flap-using-adipose-derived-mesenchymal-stem-cells-in-situ-revascularization
#6
Michael H Carstens, Mauricio Mendieta, Cecilia Pérez, Esperanza Villareal, Rodolfo Garcia
Adipose-derived mesenchymal stem cells (ASCs) have been shown to produce vascular endothelial growth factor (VEGF) and can increase perfusion in patients with critical limb ischemia. We will show that this concept can be applied to augment blood flow in zones of flap ischemia. We presented a case study of a 26-year-old man with a complex hand injury covered by a reverse radial perforator fasciocutaneous flap, which developed ischemic necrosis and was treated by debridement, transplantation of ASCs to enhance vascular support, and saline dressings...
July 1, 2017: Aesthetic Surgery Journal
https://www.readbyqxmd.com/read/29025214/effect-feasibility-and-clinical-relevance-of-cell-enrichment-in-large-volume-fat-grafting-a-systematic-review
#7
Bo Sonnich Rasmussen, Celine Lykke Sørensen, Peter Viktor Vester-Glowinski, Mikkel Herly, Stig-Frederik Trojahn Kølle, Anne Fischer-Nielsen, Krzysztof Tadeusz Drzewiecki
Large volume fat grafting is limited by unpredictable volume loss; therefore, methods of improving graft retention have been developed. Fat graft enrichment with either stromal vascular fraction (SVF) cells or adipose tissue-derived stem/stromal cells (ASCs) has been investigated in several animal and human studies, and significantly improved graft retention has been reported. Improvement of graft retention and the feasibility of these techniques are equally important in evaluating the clinical relevance of cell enrichment...
July 1, 2017: Aesthetic Surgery Journal
https://www.readbyqxmd.com/read/29025212/use-of-freshly-isolated-human-adipose-stromal-cells-for-clinical-applications
#8
Ryan A Lockhart, Joel A Aronowitz, Severiano Dos-Anjos Vilaboa
The clinical use of adipose-derived cells is being explored very actively around the world for various human diseases. Adipose tissue is an abundant tissue source that can be easily harvested using liposuction. Human lipoaspirates contain a significant amount of mesenchymal stromal cells, as well as other progenitors and terminally differentiated cell types. This review covers the isolation of adipose stromal vascular fraction (SVF), the quality control and safety analysis of freshly isolated cell suspensions...
July 1, 2017: Aesthetic Surgery Journal
https://www.readbyqxmd.com/read/29023156/stem-cell-therapy-for-articular-cartilage-repair-review-of-the-entity-of-cell-populations-used-and-the-result-of-the-clinical-application-of-each-entity
#9
Yong-Beom Park, Chul-Won Ha, Ji Heon Rhim, Han-Jun Lee
BACKGROUND: Following successful preclinical studies, stem cell therapy is emerging as a candidate for the treatment of articular cartilage lesions. Because stem cell therapy for cartilage repair in humans is at an early phase, confusion and errors are found in the literature regarding use of the term stem cell therapy in this field. PURPOSE: To provide an overview of the outcomes of cartilage repair, elucidating the various cell populations used, and thus reduce confusion with regard to using the term stem cell therapy...
October 1, 2017: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/28994031/differential-adipose-tissue-proteomics
#10
Kelly J Shields, Changgong Wu
Differential proteomic analysis (comparative quantitative proteomics) is a robust quantitative technique used to detect and identify the proteome of selected tissues. The expression levels (upregulated vs. downregulated) of proteins in tissue samples that differ by experimental design or anatomic location are determined by a series of assays including (1) 2D difference gel electrophoresis (2D-DiGE), (2) protein spot picking based on a priori thresholds, (3) Mass Spectrometry, and (4) follow-up Western Blot for antibody validation (Chen et al...
October 10, 2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28993845/-new-experimental-strategies-in-cartilage-surgery
#11
REVIEW
L Rackwitz, J C Reichert, O Pullig, U Nöth
BACKGROUND: Cell and growth factor based strategies bear great potential to support the healing processes in cartilage repair and the therapy of osteoarthritic joints. OBJECTIVES: The following review provides an overview of novel experimental strategies for the therapy of focal cartilage defects and osteoarthritis, with emphasis on cell and growth factor based approaches. MATERIALS AND METHODS: The authors summarize their own data regarding the intraarticular injection of stem cells to treat osteoarthritis of the knee and provide a synopsis of the available literature discussing the most significant publications...
October 9, 2017: Der Orthopäde
https://www.readbyqxmd.com/read/28987658/magnetic-resonance-and-ultrasound-in-achilles-tendinopathy-predictive-role-and-response-assessment-to-platelet-rich-plasma-and-adipose-derived-stromal-vascular-fraction-injection
#12
Domenico Albano, Carmelo Messina, Federico Giuseppe Usuelli, Laura De Girolamo, Miriam Grassi, Camilla Maccario, Bianca Bignotti, Alberto Tagliafico, Luca Maria Sconfienza
OBJECTIVE: To assess the correlation between magnetic resonance and ultrasound findings and clinical outcome after intratendinous injection of leucocyte-rich platelet-rich plasma or adipose-derived stromal vascular fraction in patients with non-insertional Achilles tendinopathy. MATERIALS AND METHODS: Forty-three patients (age: 47.8±5.1, range 29-55) with unilateral or bilateral non-insertional Achilles tendinopathy (58 tendons overall) were randomly assigned to platelet-rich plasma (22 patients, 28 tendons) or adipose-derived stromal vascular fraction (21 patients, 30 tendons) injection group...
October 2017: European Journal of Radiology
https://www.readbyqxmd.com/read/28984178/effects-of-donor-age-on-human-adipose-derived-adherent-stromal-cells-under-oxidative-stress-conditions
#13
Sang Woo Kim, Jung-Won Choi, Chang Youn Lee, Jiyun Lee, Sunhye Shin, Soyeon Lim, Seahyoung Lee, Il-Kwon Kim, Hoon-Bum Lee, Ki-Chul Hwang
Objective Adipose-derived stromal vascular fractions (SVFs) are heterogeneous complex populations of cells with therapeutic efficacy for tissue generation and vascular stabilization. SVFs have cardiomyogenic potential, and many researchers have examined the possibility of SVF transplantation for heart disease. In cell-based therapies, donor age affects the regenerative capability, cell yield, and differentiation potential of adult tissues; however, opposing or controversial results have been found in humans...
January 1, 2017: Journal of International Medical Research
https://www.readbyqxmd.com/read/28967436/the-angiogenic-properties-of-human-adipose-derived-stem-cells-hascs-are-modulated-by-the-high-mobility-group-box-protein-1-hmgb1
#14
Federico Biscetti, Stefano Gentileschi, Flavio Bertucci, Maria Servillo, Vincenzo Arena, Flavia Angelini, Egidio Stigliano, Giuseppina Bonanno, Giovanni Scambia, Benedetto Sacchetti, Luca Pierelli, Raffaele Landolfi, Andrea Flex
Peripheral arterial disease (PAD), is a major health problem. Many studies have been focused on the possibilities of treatment offered by vascular regeneration. Human adipose-derived stem cells (HASCs), multipotent CD34+ stem cells found in the stromal-vascular fraction of adipose tissues, which are capable to differentiate into multiple mesenchymal cell types. The High mobility group box 1 protein (HMGB1) is a nuclear protein involved in angiogenesis. The aim of the study was to define the role of HMGB1 in cell therapy with HASCs, in an animal model of PAD...
September 22, 2017: International Journal of Cardiology
https://www.readbyqxmd.com/read/28957413/characterization-of-immortalized-human-brown-and-white-pre-adipocyte-cell-models-from-a-single-donor
#15
Lasse K Markussen, Marie S Isidor, Peter Breining, Elise S Andersen, Nanna E Rasmussen, Louise I Petersen, Steen B Pedersen, Bjørn Richelsen, Jacob B Hansen
Brown adipose tissue with its constituent brown adipocytes is a promising therapeutic target in metabolic disorders due to its ability to dissipate energy and improve systemic insulin sensitivity and glucose homeostasis. The molecular control of brown adipocyte differentiation and function has been extensively studied in mice, but relatively little is known about such regulatory mechanisms in humans, which in part is due to lack of human brown adipose tissue derived cell models. Here, we used retrovirus-mediated overexpression to stably integrate human telomerase reverse transcriptase (TERT) into stromal-vascular cell fractions from deep and superficial human neck adipose tissue biopsies from the same donor...
2017: PloS One
https://www.readbyqxmd.com/read/28949833/fifty-shades-of-white-understanding-heterogeneity-in-white-adipose-stem-cells
#16
Louise Cleal, Teodora Aldea, You-Ying Chau
The excessive expansion of white adipose tissue underlies the global obesity epidemic. However, not all fat is equal, and the impact of heterogeneity on the development and expansion of different adipose depots is becoming increasingly apparent. Two mechanisms are responsible for the growth of adipose tissue: hyperplasia (increasing adipocyte number) and hypertrophy (increasing adipocyte size). The former relies on the differentiation of adipocyte stem cells, which reside within the adipose stromal vascular fraction...
July 3, 2017: Adipocyte
https://www.readbyqxmd.com/read/28941163/colorectal-wall-regeneration-resulting-from-the-association-of-chitosan-hydrogel-and-stromal-vascular-fraction-from-adipose-tissue
#17
Quentin Denost, Arnaud Pontallier, Etienne Buscail, Alexandra Montembault, Reine Bareille, Robin Siadous, Marlene Durand, Samantha Delmond, Laurent David, Laurence Bordenave
Chitosan hydrogel and Adipose Derived Stem Cells (ADCS) have been reported as the optimal partnership for colorectal tissue engineering. In that field, the aim of the current experiment was to asses, the interest of seeding ADSC on chitosan hydrogel patches in an in vivo comparative study and on a tube intended replace a colonic segment in an in vivo feasibility study. In the comparative study, a 2x3 cm colonic wall defect was performed in 20 swines and repaired by suturing a chitosan hydrogel patch: acellular matrix (Group A, n=10) vs...
September 22, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28938327/adipose-tissue-derived-stromal-vascular-fraction-increases-osteogenesis-in-an-experimental-design-zygomatic-bone-defect-model
#18
Gaye Toplu, Derya Ozcelik, Merdan Serin, Havva Erdem, Arzu Turkseven Topacoglu
INTRODUCTION: Facial bone defects are frequently encountered problems in clinical practice. Bone grafts, flaps, and alloplastic materials are often used in their treatment. This leads to donor site morbidity and prolongation of the operation. The authors have planned this study to examine whether adipose tissue derived stromal vascular fraction (SVF) has an osteogenic effect in the critical sized membranous bone defect of the zygomatic bone. MATERIALS AND METHODS: Twenty male Wistar Albino rats were used...
September 21, 2017: Journal of Craniofacial Surgery
https://www.readbyqxmd.com/read/28922324/biological-properties-and-therapeutic-value-of-cryopreserved-fat-tissue
#19
Takanobu Mashiko, Szu-Hsien Wu, Koji Kanayama, Rintaro Asahi, Takako Shirado, Masanori Mori, Ataru Sunaga, Shunji Sarukawa, Hirokazu Uda, Kotaro Yoshimura
BACKGROUND: Fat grafting frequently requires multiple treatments and thus repeated liposuction to achieve treatment goals. The purpose of this study is to evaluate whether cryopreservation of adipose tissue may facilitate future fat grafting. METHODS: Lipoaspirates were harvested from six adult females and preserved using two cryopreservation methods: 1) simple cooling to -80˚C (cryo-1); or 2) programmed cooling to -190˚C (cryo-2). Fresh fat, cryo-1 fat, and cryo-2 fat were analyzed both in vitro and in vivo...
September 14, 2017: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/28915327/adipose-tissue-in-hiv-infection
#20
John R Koethe
HIV infection and antiretroviral therapy (ART) treatment exert diverse effects on adipocytes and stromal-vascular fraction cells, leading to changes in adipose tissue quantity, distribution, and energy storage. A HIV-associated lipodystrophic condition was recognized early in the epidemic, characterized by clinically apparent changes in subcutaneous, visceral, and dorsocervical adipose depots. Underlying these changes is altered adipose tissue morphology and expression of genes central to adipocyte maturation, regulation, metabolism, and cytokine signaling...
September 12, 2017: Comprehensive Physiology
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