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https://www.readbyqxmd.com/read/28046109/dietary-yeast-cell-wall-extract-alters-the-proteome-of-the-skin-mucous-barrier-in-atlantic-salmon-salmo-salar-increased-abundance-and-expression-of-a-calreticulin-like-protein
#1
Giulia Micallef, Phillip Cash, Jorge M O Fernandes, Binoy Rajan, John W Tinsley, Ralph Bickerdike, Samuel A M Martin, Alan S Bowman
In order to improve fish health and reduce use of chemotherapeutants in aquaculture production, the immunomodulatory effect of various nutritional ingredients has been explored. In salmon, there is evidence that functional feeds can reduce the abundance of sea lice. This study aimed to determine if there were consistent changes in the skin mucus proteome that could serve as a biomarker for dietary yeast cell wall extract. The effect of dietary yeast cell wall extract on the skin mucus proteome of Atlantic salmon was examined using two-dimensional gel electrophoresis...
2017: PloS One
https://www.readbyqxmd.com/read/28026022/protective-responses-of-intestinal-mucous-cells-in-a-range-of-fish-helminth-systems
#2
G Bosi, L Giari, J A DePasquale, A Carosi, M Lorenzoni, B Sayyaf Dezfuli
Histopathological, immunofluorescence and ultrastructural studies were conducted on the intestines of four fish species infected with different taxa of enteric helminths. Brown trout (Salmo trutta trutta), eel (Anguilla anguilla) and tench (Tinca tinca) obtained from Lake Piediluco (central Italy) were examined. Brown trout and eel were infected with two species of acanthocephalans, and tench was parasitized with a tapeworm species. In addition to the above site, specimens of chub (Squalius cephalus) and brown trout infected with an acanthocephalan were examined from the River Brenta (north Italy)...
December 27, 2016: Journal of Fish Diseases
https://www.readbyqxmd.com/read/28009054/new-icing-media-for-quality-enhancement-of-chilled-hake-merluccius-merluccius-using-a-jumbo-squid-dosidicus-gigas-skin-extract
#3
Josafat Marina Ezquerra-Brauer, José M Miranda, Jesús Enrique Chan-Higuera, Jorge Barros-Velázquez, Santiago P Aubourg
BACKGROUD: An advanced strategy for chilled fish preservation, based on the inclusion in ice of an extract of jumbo squid (Dosidicus gigas) skin (JSS), is proposed. Aqueous solutions including acetic acid-ethanol extracts of JSS were tested at two different concentrations as icing media, being the effects on the quality evolution of chilled hake (Merluccius merluccius) monitored. RESULTS: A significant inhibition (p < 0.05) of microbial activity (aerobes, psychrotrophs, Enterobacteriaceae, proteolytic bacteria; pH, trimethylamine) was obtained in hake corresponding to the icing batch including the highest JSS concentration...
December 23, 2016: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/27994904/bronchial-foreign-body-alerting-of-a-bronchial-tumor-the-need-of-a-follow-up-radiography
#4
Nahida El-Rifai, Samar Shahine, Hassan Sidani, Ali Sabeh Aion, Antoine Deschildre, Marie-Christine Copin
Lung tumors are extremely rare in the pediatric population, comprising only 0.2% of all malignancies in children. Among them, mucoepidermoid carcinoma (MEC) is even rarer with a reported frequency of 0.1% to 0.2%. MEC is defined by the World Health Organization as a tumor characterized by a combination of mucus-secreting, squamous, and intermediate cell types. We describe the case of a 4-year-old girl who presented with a history of intermittent fever and nonproductive cough of 1-month duration after foreign body aspiration...
2016: Case Reports in Pediatrics
https://www.readbyqxmd.com/read/27992807/cellular-stress-and-histopathological-tools-used-as-biomarkers-in-oreochromis-mossambicus-for-assessing-metal-contamination
#5
Neeraj Kumar, K K Krishnani, S K Gupta, N P Singh
In the present study, we have analyzed various biochemical and histopathological biomarkers in the Oreochromis mossambicus collected from 18 sites at Bhima River. The biochemical biomarkers viz. antioxidative enzymes in muscle viz. catalase, SOD, and GST, glycolytic enzymes in kidney viz. LDH and MDH, protein metabolic enzymes in liver, gill, brain, gonad and muscle viz. ALT and AST, neurotransmitter enzymes in muscle AChE, lipid peroxidation and histopathology in the liver and gill have been evaluated. The oxidative biomarkers, glycolytic enzymes, protein metabolic enzymes and lipid peroxidation were significantly (p<0...
November 24, 2016: Environmental Toxicology and Pharmacology
https://www.readbyqxmd.com/read/27966609/symbiont-derived-sphingolipids-modulate-mucosal-homeostasis-and-b-cells-in-teleost-fish
#6
Ali Sepahi, Héctor Cordero, Howard Goldfine, Maria Ángeles Esteban, Irene Salinas
Symbiotic bacteria and mucosal immunoglobulins have co-evolved for millions of years in vertebrate animals. Symbiotic bacteria products are known to modulate different aspects of the host immune system. We recently reported that Flectobacillus major is a predominant species that lives in the gill and skin mucosal surfaces of rainbow trout (Oncorhynchus mykiss). F. major is known to produce sphingolipids of a unique molecular structure. Here we propose a role for F. major and its sphingolipids in the regulation of B cell populations in rainbow trout, as well as an essential role for sphingolipids in trout mucosal homeostasis...
December 14, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27898676/dietary-butyrate-helps-to-restore-the-intestinal-status-of-a-marine-teleost-sparus-aurata-fed-extreme-diets-low-in-fish-meal-and-fish-oil
#7
Itziar Estensoro, Gabriel Ballester-Lozano, Laura Benedito-Palos, Fabian Grammes, Juan Antonio Martos-Sitcha, Liv-Torunn Mydland, Josep Alvar Calduch-Giner, Juan Fuentes, Vasileios Karalazos, Álvaro Ortiz, Margareth Øverland, Ariadna Sitjà-Bobadilla, Jaume Pérez-Sánchez
There is a constant need to find feed additives that improve health and nutrition of farmed fish and lessen the intestinal inflammation induced by plant-based ingredients. The objective of this study was to evaluate the effects of adding an organic acid salt to alleviate some of the detrimental effects of extreme plant-ingredient substitution of fish meal (FM) and fish oil (FO) in gilthead sea bream diet. Three experiments were conducted. In a first trial (T1), the best dose (0.4%) of sodium butyrate (BP-70 ®NOREL) was chosen after a short (9-weeks) feeding period...
2016: PloS One
https://www.readbyqxmd.com/read/27890799/enhancing-gilthead-seabream-immune-status-and-protection-against-bacterial-challenge-by-means-of-antigens-derived-from-vibrio-parahaemolyticus
#8
Martha Reyes-Becerril, Crystal Guluarte, Diana Ceballos-Francisco, Carlos Angulo, M Ángeles Esteban
In an attempt to control the proliferation of the pathogenic bacterium Vibrio parahaemolyticus in gilthead seabream (Sparus aurata), the immunostimulant effect of lysate and ToxA from this bacterium was evaluated. Fish were intraperitoneally injected twice (first injection, day 1 of the experiment; second injection, day 7) and sampled after one week (on days 8 and 15). Afterwards, all fish specimens were experimentally infected with V. parahaemolyticus and mortality was recovered for 1 week. Fish injected with lysate, ToxA and phosphate buffer saline (control) showed 100%, 50% and 0% survival, respectively, when challenged with the pathogen...
November 25, 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27836718/terminal-carbohydrates-abundance-immune-related-enzymes-bactericidal-activity-and-physico-chemical-parameters-of-the-senegalese-sole-solea-senegalensis-kaup-skin-mucus
#9
Francisco A Guardiola, María Cuartero, María Del Mar Collado-González, F Guillermo Díaz Baños, Alberto Cuesta, Miguel Ángel Moriñigo, M Ángeles Esteban
Recently, interest in mucosal surfaces, more specifically fish skin and its secreted mucus, has greatly increased among immunologists. The abundance of terminal carbohydrates, several enzymes (proteases, lysozyme, peroxidase, alkaline phosphatase, esterases and ceruloplasmin), bactericidal activity against fish pathogenic and non-pathogenic bacteria and several physico-chemical parameters (protein concentration, pH, conductivity, redox potential, osmolarity, density and viscosity) in the skin mucus of Senegalese sole (Solea senegalensis, Kaup) have been evaluated...
November 9, 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27818341/using-skin-mucus-to-evaluate-stress-in-gilthead-seabream-sparus-aurata-l
#10
Francisco A Guardiola, Alberto Cuesta, M Ángeles Esteban
The welfare of farmed fish is influenced by environmental and management factors, which may substantially increase stress levels of the animals and even endanger their survival. Three experiments to simulate different stress conditions (acute crowding, anaesthetic agents and air exposure) were developed. Cortisol levels were measured in fish skin mucus and the values obtained were correlated with those obtained in serum from the same fish specimens and also in the water surrounding the fish. Concomitantly, the effect of these stressors on immunoglobulin M (IgM) and several enzymes related to immunity were also determined in the serum and skin mucus of gilthead seabream (Sparus aurata L...
December 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27815737/studies-on-histopathological-alteration-of-three-major-organs-of-the-goldfish-carassius-auratus-l-of-india-due-to-myxozoan-infection-with-special-reference-to-scanning-electron-microscopic-observation
#11
Mandira Saha, P K Bandyopadhyay
Severe infections by the protozoan parasite belonging to the genera Myxozoa are causing serious problems in ornamental fish reared in breeding farms. Histopathological study is being used for diagnosis of the severity of diseases. Myxozoan infections result in large scale histopathological damages in different fish tissues. No information is available regarding the histopathological changes of goldfish due to infection with myxozoans. The present study deals with the histopathological changes of the gill, fin, and skin of goldfish, infected with myxozoan parasites along with an ultrastructural study using scanning electron microscope...
November 5, 2016: Parasitology Research
https://www.readbyqxmd.com/read/27808112/fish-gut-liver-immunity-during-homeostasis-or-inflammation-revealed-by-integrative-transcriptome-and-proteome-studies
#12
Nan Wu, Yu-Long Song, Bei Wang, Xiang-Yang Zhang, Xu-Jie Zhang, Ya-Li Wang, Ying-Yin Cheng, Dan-Dan Chen, Xiao-Qin Xia, Yi-Shan Lu, Yong-An Zhang
The gut-associated lymphoid tissue, connected with liver via bile and blood, constructs a local immune environment of both defense and tolerance. The gut-liver immunity has been well-studied in mammals, yet in fish remains largely unknown, even though enteritis as well as liver and gallbladder syndrome emerged as a limitation in aquaculture. In this study, we performed integrative bioinformatic analysis for both transcriptomic (gut and liver) and proteomic (intestinal mucus and bile) data, in both healthy and infected tilapias...
November 3, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27796002/digestive-physiological-characteristics-of-the-gobiidae-characteristics-of-cck-producing-cells-and-mucus-secreting-goblet-cells-of-stomach-fish-and-stomachless-fish
#13
Sang-Woo Hur, Shin-Kwon Kim, Dae-Jung Kim, Bae-Ik Lee, Su-Jin Park, Hyung-Gyu Hwang, Je-Cheon Jun, Jeong-In Myeong, Chi-Hoon Lee, Young-Don Lee
In this study, we investigated the characteristics of CCK-producing cells and mucus-secreting goblet cells with respect to stomach fish and stomachless fish of the Gobiidae in order to provide a basis for understanding the digestive physiology. Hairychin goby (Sagamia geneionema), which is stomachless fish, the numbers of mucus-secreting goblet cells is highest in the posterior intestine portion (P<0.05), while CCK-producing cells are scattered throughout the intestine. Gluttonous goby (Chasmichthys gulosus), which is stomach fish, mucus-secreting goblet cells are most abundant in the mid intestine portion (P<0...
September 2016: Balsaeng'gwa Saengsig
https://www.readbyqxmd.com/read/27787214/a-new-species-of-trichodina-ehrenberg-1830-ciliophora-trichodinidae-from-rhamdia-quelen-siluriformes-heptapteridae-and-gymnotus-sp-teleostei-gymnotidae-in-brazil
#14
Gabriela S O Hashimoto, Natalia C Marchiori, Santiago B Pádua, Márcia M Ishikawa, Juan R E Garcia, Maurício L Martins
The present study describes a new species of Trichodina found in the mucus of the body surface of 137 farmed and wild silver catfish (Rhamdia quelen) located in southern Brazil and in the gills of 99 banded knife fish (Gymnotus sp.) captured in the basin of Pantanal. Smears of the body surface mucus and gills were air dried at room temperature and impregnated with silver nitrate and stained with Giemsa for analysis of the adhesive disc, denticles and macronucleus. Characterized as a medium trichodinid, Trichodina quelenii n...
December 1, 2016: Acta Parasitologica
https://www.readbyqxmd.com/read/27786156/pathology-of-finfish-and-mud-crabs-scylla-serrata-during-a-mortality-event-associated-with-a-harbour-development-project-in-port-curtis-australia
#15
M M Dennis, B K Diggles, R Faulder, L Olyott, S B Pyecroft, G E Gilbert, M Landos
The objective of this study was to assess the extent and describe the nature of a multi-species marine finfish and crustacean disease event that occurred in Gladstone Harbour, Australia, 2011-2012. Finfish were examined for this study in January to April 2012 from sites where diseased animals were previously observed by the public. Gross abnormalities, including excessive skin and gill mucus, erythema, heavy ecto-parasitism, cutaneous ulceration, corneal opacity, and exophthalmos, were higher (25.5%) in finfish from Gladstone Harbour (n = 435) than in those from an undeveloped reference site, 250 km to the north (5...
October 27, 2016: Diseases of Aquatic Organisms
https://www.readbyqxmd.com/read/27769690/dietary-lactobacillus-acidophilus-modulated-skin-mucus-protein-profile-immune-and-appetite-genes-expression-in-gold-fish-carassius%C3%A2-auratus-gibelio
#16
Marjan Hosseini, Hamed Kolangi Miandare, Seyed Hossein Hoseinifar, Peyman Yarahmadi
The objective of the present study was to investigate the effect of dietary Lactobacillus acidophilus on skin mucus protein pattern, immune and appetite related genes expression as well as growth performance in gold fish (Carassius auratus gibelio). Three hundred healthy gold fish (2.5 ± 0.05) juveniles were randomly distributed in 12 glass aquariums (400-L; 25 fish per aquaria) and fed experimental diets contain different levels of L. acidophilus (0, 1.5 × 10(8), 3 × 10(8) and 6 × 10(8)) for 8 weeks...
December 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27751916/dietary-phytoimmunostimulant-persian-hogweed-heracleum-persicum-has-more-remarkable-impacts-on-skin-mucus-than-on-serum-in-common-carp-cyprinus-carpio
#17
Seyed Hossein Hoseinifar, Fazel Zoheiri, Carlo C Lazado
Immunostimulation through the use of sustainable and eco-friendly dietary additives is one of the current prophylactic strategies in fish husbandry. Plant-based immunostimulants are highly considered for this intent, both for their scientific and practical advantages. Persian hogweed (Heracleum persicum) is a flower-bearing herb that possesses interesting pharmacological importance due to its bioactive compounds. It is commonly used as spice, food additive, dietary supplement and traditional remedy. The present study evaluated the potential of H...
December 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27746382/association-between-opisthorchis-viverrini-and-leptospira-spp-infection-in-endemic-northeast-thailand
#18
Chinh Dang Van, Galayanee Doungchawee, Sutas Suttiprapa, Yuji Arimatsu, Sasithorn Kaewkes, Banchob Sripa
Opisthorchiasis caused by Opisthorchis viverrini is an important foodborne trematodiasis in Thailand, Laos and Cambodia. Interestingly, the opisthorchiasis endemic region overlaps with an area of leptospirosis emergence. Here we report an association between opisthorchiasis and leptospirosis in Thailand. Of 280 sera collected from villagers living around the Lawa wetland complex in Khon Kaen province, 199 (71%) were seropositive for leptospirosis by immunochromatography. Individuals with O. viverrini infection had a significantly higher rate of leptospirosis than those without (P=0...
October 13, 2016: Parasitology International
https://www.readbyqxmd.com/read/27729275/immune-response-induced-by-oral-delivery-of-bacillus-subtilis-spores-expressing-enolase-of-clonorchis-sinensis-in-grass-carps-ctenopharyngodon-idellus
#19
Hongye Jiang, Tingjin Chen, Hengchang Sun, Zeli Tang, Jinyun Yu, Zhipeng Lin, Pengli Ren, Xinyi Zhou, Yan Huang, Xuerong Li, Xinbing Yu
Clonorchiasis, caused by the consumption of raw or undercooked freshwater fish containing infective metacercariae of Clonorchis sinensisis (C.sinensis), remains a common public health problem. New effective prevention strategies are still urgent to control this food-borne infectious disease. The previous studies suggested Bacillus subtilis (B. subtilis) spores was an ideal vaccines delivery system, and the C.sinensis enolase (CsENO) was a potential vaccine candidate against clonorchiasis. In the current study, we detected CsENO-specific IgM levels by ELISA in sera, intestinal mucus and skin mucus in grass carps (Ctenopharyngodon idella) through oral administration with B...
October 8, 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27699803/quorum-sensing-involved-in-the-spoilage-process-of-the-skin-and-flesh-of-vacuum-packaged-farmed-turbot-scophthalmus-maximus-stored-at-4-%C3%A2-c
#20
Caili Zhang, Suqin Zhu, Haohao Wu, Abdul-Nabi Jatt, Yurong Pan, Mingyong Zeng
Fish skin has both positive and negative effects on the shelf-life of the fish. This study aimed to investigate the spoilage process of the skin and flesh of refrigerated farmed turbot (Scophthalmus maximus) with vacuum packaging. Microbial community changes were analyzed by combining culture-dependent and culture-independent methods. The results indicated that the shelf-life of vacuum-packaged refrigerated turbot was 16 d; skin mucus was the interference factor of turbot quality. The culture-dependent analysis demonstrated that the total viable counts and the population of H2 S-producing bacteria, Pseudomonas spp...
October 3, 2016: Journal of Food Science
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