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https://www.readbyqxmd.com/read/29898095/brazilian-legislation-on-genetic-heritage-harms-biodiversity-convention-goals-and-threatens-basic-biology-research-and-education
#1
EDITORIAL
Ruy José V Alves, Marcelo Weksler, João A Oliveira, Paulo A Buckup, José P Pombal, Hélcio R G Santana, Adriano Lúcio Peracchi, Alexander W A Kellner, Alexandre Aleixo, Alfredo Ricardo L Bonino, Alzira Maria P DE Almeida, Ana Luisa Albernaz, Camila C Ribas, Carla Zilberberg, Carlos Eduardo V Grelle, Carlos Frederico D DA Rocha, Carlos José E Lamas, Célio Fernando B Haddad, Cibele R Bonvicino, Cynthia P A Prado, Daniela O DE Lima, Denise C Rossa-Feres, Fabrício R Dos Santos, Fátima Regina G Salimena, Fernando A Perini, Flávio A Bockmann, Francisco Luís Franco, Gisele M L Del Giudice, Guarino R Colli, Ima Célia G Vieira, Jader Marinho-Filho, Jane M C F Werneck, Jorge A D Dos Santos, Jorge Luiz DO Nascimento, Jorge Luiz Nessimian, José Luis P Cordeiro, Kleber Del Claro, Leandro O Salles, Lilian Casatti, Lucia Helena R Py-Danie, Luís Fábio Silveira, Luís Felipe Toledo, Luiz F DE Oliveira, Luiz Roberto Malabarba, Marcelo D DA Silva, Márcia S Couri, Márcio R C Martins, Marcos D S Tavares, Marcos Eduardo G Sobral, Marcus Vinícius Vieira, Maria DE Lourdes A Oliveira, Mário César C DE Pinna, Michael J G Hopkins, Mirco Solé, Naércio A Menezes, Paulo Passos, Paulo Sergio D'Andrea, Pedro C E A Pinto, Pedro L Viana, Peter M Toledo, Roberto E Dos Reis, Roberto Vilela, Rogério P Bastos, Rosane G Collevatti, Rui C Silva, Santiago C Fisher, Ulisses Caramaschi
No abstract text is available yet for this article.
April 2018: Anais da Academia Brasileira de Ciências
https://www.readbyqxmd.com/read/29896184/the-role-of-candida-albicans-transcription-factor-rlm1-in-response-to-carbon-adaptation
#2
João Oliveira-Pacheco, Rosana Alves, Augusto Costa-Barbosa, Bruno Cerqueira-Rodrigues, Patricia Pereira-Silva, Sandra Paiva, Sónia Silva, Mariana Henriques, Célia Pais, Paula Sampaio
Candida albicans is the main causative agent of candidiasis and one of the most frequent causes of nosocomial infections worldwide. In order to establish an infection, this pathogen supports effective stress responses to counter host defenses and adapts to changes in the availability of important nutrients, such as alternative carbon sources. These stress responses have clear implications on the composition and structure of Candida cell wall. Therefore, we studied the impact of lactate, a physiologically relevant carbon source, on the activity of C...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29849872/methyl-chavicol-and-its-synthetic-analogue-as-possible-antioxidant-and-antilipase-agents-based-on-the-in-vitro-and-in-silico-assays
#3
Bruna Celeida Silva Santos, Andressa Soares Pires, Célia Hitomi Yamamoto, Mara Rubia Costa Couri, Alex Gutterres Taranto, Maria Silvana Alves, Ana Lúcia Dos Santos de Matos Araújo, Orlando Vieira de Sousa
This study investigated the in vitro and in silico biological properties of the methyl chavicol (MC) and its analogue 2-[(4-methoxyphenyl)methyl]oxirane (MPMO), emphasizing the antioxidant and antilipase effects. MPMO was synthesized from MC that reacted with meta -chloroperbenzoic acid and, after separation and purification, was identified by 1 H and 13 C NMR and GC-MS. The antioxidant activity was investigated by DPPH, cooxidation β -carotene/linoleic acid, and thiobarbituric acid assays. With the use of colorimetric determination, the antilipase effect on the pancreatic lipase was tested, while the molecular interaction profiles were evaluated by docking molecular study...
2018: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/29801795/in-vitro-ex-vivo-and-in-vivo-models-a-comparative-analysis-of-paracoccidioides-spp-proteomic-studies
#4
Juliana Alves Parente-Rocha, Mariana Vieira Tomazett, Laurine Lacerda Pigosso, Alexandre Melo Bailão, Aparecido Ferreira de Souza, Juliano Domiraci Paccez, Lilian Cristiane Baeza, Maristela Pereira, Mirelle Garcia Silva Bailão, Clayton Luiz Borges, Célia Maria de Almeida Soares
Members of the Paracoccidioides complex are human pathogens that infect different anatomic sites in the host. The ability of Paracoccidioides spp. to infect host niches is putatively supported by a wide range of virulence factors, as well as fitness attributes that may comprise the transition from mycelia/conidia to yeast cells, response to deprivation of micronutrients in the host, expression of adhesins on the cell surface, response to oxidative and nitrosative stresses, as well as the secretion of hydrolytic enzymes in the host tissue...
June 2018: Fungal Biology
https://www.readbyqxmd.com/read/29791639/validity-and-reliability-of-the-brazilian-version-of-the-caregiver-reaction-assessment
#5
Fernanda Rochelly do Nascimento Mota, Janaína Fonseca Victor, Maria Josefina da Silva, Edson Silva Soares, Mônica Oliveira Batista Oriá, Marília Braga Marques, Maria Célia de Freitas, Allana Mirella Alves
OBJECTIVE: To analyze the validity based on the internal structure and reliability of the Brazilian version of the Caregiver Reaction Assessment (CRA) applied to informal caregivers of dependent elderly. METHOD: A methodological study with 120 informal caregivers of the elderly. Validity based on the internal structure evaluated by exploratory factorial analysis, with extraction of main factorial axes, varimax orthogonal rotation and retention of factors by parallel analysis...
2018: Revista Brasileira de Enfermagem
https://www.readbyqxmd.com/read/29748136/blocking-ip-3-signal-transduction-pathways-inhibits-melatonin-induced-ca-2-signals-and-impairs-p-falciparum-development-and-proliferation-in-erythrocytes
#6
Mateus Fila Pecenin, Lucas Borges-Pereira, Julio Levano-Garcia, Alexandre Budu, Eduardo Alves, Katsuhiko Mikoshiba, Andrew Thomas, Celia R S Garcia
Inositol 1,4,5 trisphosphate (IP3 ) signaling plays a crucial role in a wide range of eukaryotic processes. In Plasmodium falciparum, IP3 elicits Ca2+ release from intracellular Ca2+ stores, even though no IP3 receptor homolog has been identified to date. The human host hormone melatonin plays a key role in entraining the P. falciparum life cycle in the intraerythrocytic stages, apparently through an IP3 -dependent Ca2+ signal. The melatonin-induced cytosolic Ca2+ ([Ca2+ ]cyt ) increase and malaria cell cycle can be blocked by the IP3 receptor blocker 2-aminoethyl diphenylborinate (2-APB)...
March 14, 2018: Cell Calcium
https://www.readbyqxmd.com/read/29625021/hepatitis-c-viral-load-in-hcv-monoinfected-and-hcv-hiv-1-hcv-htlv-1-2-and-hcv-hiv-htlv-1-2-co-infected-patients-from-s%C3%A3-o-paulo-brazil
#7
Fabiana Aparecida Alves, Karoline Rodrigues Campos, Marcílio Figueiredo Lemos, Regina Célia Moreira, Adele Caterino-de-Araujo
Co-infections of hepatitis C virus (HCV) and either human immunodeficiency virus type 1 (HIV-1), human T-cell lymphotropic virus type 1 (HTLV-1) or type 2 (HTLV-2) have been described as having an impact on HCV viremia and subsequent disease progression. HCV load in serum samples from 622 patients (343 males, 279 females; median age 50.8 years) from São Paulo/southeast Brazil was analyzed using the Abbott Real Time HCV assay (Abbott Molecular Inc., IL, USA). Samples were obtained from HCV-monoinfected (n=548), HCV/HIV-1- (n=41), HCV/HTLV-1- (n=16), HCV/HTLV-2- (n=8), HCV/HIV/HTLV-1- (n=4), and HCV/HIV/HTLV-2-co-infected (n=5) patients, and results were compared among the groups and according to sex...
March 2018: Brazilian Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29546667/proteomic-analysis-of-a-549-cells-infected-with-human-adenovirus-40-by-lc-ms
#8
Ana Carla Peixoto Guissoni, Célia Maria Almeida Soares, Kareem R Badr, Fabiola Sousa Ficcadori, Ana Flávia Alves Parente, Juliana Alves Parente, Lilian Cristina Baeza, Menira Souza, Divina das Dores de Paula Cardoso
Human Adenoviruses (HAdVs) are etiological agents of different syndromes such as gastroenteritis, cystitis, ocular, and respiratory diseases, and infection by these viruses may cause alterations in cellular homeostasis. The objective of the study was the proteomic analysis of A-549 cells infected with HAdV-40 using LC-MS. At 30 h of infection, the quantitative analysis revealed 336 differentially expressed proteins. From them, 206 were induced (up-regulated) and 130 were suppressed (down-regulated). The majority of up-regulated proteins were related to energy, cellular organization, stress response, and apoptosis pathways...
June 2018: Virus Genes
https://www.readbyqxmd.com/read/29526166/distinct-genetic-profiles-of-leishmania-viannia-braziliensis-associate-with-clinical-variations-in-cutaneous-leishmaniasis-patients-from-an-endemic-area-in-brazil
#9
Patrícia Flávia Quaresma, Cristiana Ferreira Alves de Brito, Jeronimo Marteleto Nunes Rugani, Janaína de Moura Freire, Rodrigo de Paula Baptista, Elizabeth Castro Moreno, Raquel Carvalho Gontijo, Felipe Dutra Rego, Joaquim Edemilson Diniz, Maria Norma Melo, Célia Maria Ferreira Gontijo
American tegumentary leishmaniasis (ATL) samples obtained from the lesions of patients with typical (n = 25, 29%), atypical (n = 60, 69%) or both (n = 2%) clinical manifestations were analysed by multilocus enzyme electrophoresis, hsp70 restriction-fragment length polymorphism (PCR-RFLP), hsp70 sequencing and phylogenetics methods. The hsp70 PCR-RFLP analysis revealed two different profiles whose the most samples differed from those expected for Leishmania braziliensis and the other Leishmania species tested: of 39 samples evaluated, two (5%) had a restriction profile corresponding to L...
March 12, 2018: Parasitology
https://www.readbyqxmd.com/read/29499552/corrigendum-to-does-outcome-measurement-of-treatment-for-substance-use-disorder-reflect-the-personal-concerns-of-patients-a-scoping-review-of-measures-recommended-in-europe-drug-alcohol-dependence-179-2017-299-308
#10
https://www.readbyqxmd.com/read/29407415/waardenburg-syndrome-novel-mutations-in-a-large-brazilian-sample
#11
Magnolia Astrid Pretell Bocángel, Uirá Souto Melo, Leandro Ucela Alves, Eliete Pardono, Naila Cristina Vilaça Lourenço, Humberto Vicente Cezar Marcolino, Paulo Alberto Otto, Regina Célia Mingroni-Netto
This paper deals with the molecular investigation of Waardenburg syndrome (WS) in a sample of 49 clinically diagnosed probands (most from southeastern Brazil), 24 of them having the type 1 (WS1) variant (10 familial and 14 isolated cases) and 25 being affected by the type 2 (WS2) variant (five familial and 20 isolated cases). Sequential Sanger sequencing of all coding exons of PAX3, MITF, EDN3, EDNRB, SOX10 and SNAI2 genes, followed by CNV detection by MLPA of PAX3, MITF and SOX10 genes in selected cases revealed many novel pathogenic variants...
January 31, 2018: European Journal of Medical Genetics
https://www.readbyqxmd.com/read/29236927/making-the-invisible-visible-searching-for-human-t-cell-lymphotropic-virus-types-1-and-2-htlv-1-and-htlv-2-in-brazilian-patients-with-viral-hepatitis-b-and-c
#12
Adele Caterino-de-Araujo, Fabiana Aparecida Alves, Karoline Rodrigues Campos, Marcílio Figueiredo Lemos, Regina Célia Moreira
With this study, the authors hope to alert clinicians regarding the presence of human T-cell lymphotropic virus type 1 and 2 (HTLV-1/-2) infections in patients with viral hepatitis B and C in Brazil. HTLV-1/-2 were detected in 1.3% of hepatitis B virus (HBV)- and 5.3% of hepatitis C virus (HCV)-infected blood samples sent for laboratory viral load measurements. A partial association of human immunodeficiency virus (HIV)-1 and HTLV-1/-2 infection was detected in patients with HCV (HIV+, 27.3%), whereas this association was almost 100% in HBV-infected patients (HIV+, all except one)...
February 2018: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/29211181/-violence-committed-by-acquaintances-brazil-2013
#13
Márcio Dênis Medeiros Mascarenhas, Raniela Borges Sinimbu, Deborah Carvalho Malta, Marta Maria Alves da Silva, Alexandre Fonseca Santos, Maria Lucia França Pontes Vieira, Célia Landmann Szwarcwald
The scope of this article is to describe the prevalence of violence committed by acquaintances in accordance with demographic characteristics. It is a descriptive study, based on data from a population-based National Health Research survey conducted in Brazil in 2013. Data from the adult population (≥ 18 years) of 64,348 households. Prevalence rates were calculated and their respective 95% confidence intervals (95%CI) according to gender, age, education, race/color, area of residence and geographical region...
November 2017: Ciência & Saúde Coletiva
https://www.readbyqxmd.com/read/29189583/modeling-fitness-variable-responses-to-training-in-prepubescent-children
#14
Ana R Alves, Carlos Marta, Henrique P Neiva, Célia Nunes, Mikel Izquierdo, Mário C Marques
The aim of this study was to determine strength and oxygen uptake (VO2max) performances according to different training program intervention design with 8-weeks duration in prepubescent children through a multiple linear regression models. Two hundred and forty-five healthy prepubescent children (aged 10.9 ± 0.5 years) were randomly assigned to a specific training program (strength training only - S; aerobic training only - A; intra-session aerobic and strength training - AS; intra-session strength and aerobic training - SA; or concurrent training performed in different sessions - CT) or a control group (no training regimen - C)...
November 16, 2017: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/29165852/capturing-and-missing-the-patient-s-story-through-outcome-measures-a-thematic-comparison-of-patient-generated-items-in-psychlops-with-core-om-and-phq-9
#15
Célia Md Sales, Inês Td Neves, Paula G Alves, Mark Ashworth
BACKGROUND: There is increasing interest in individualized patient-reported outcome measures (I-PROMS), where patients themselves indicate the specific problems they want to address in therapy and these problems are used as items within the outcome measurement tool. OBJECTIVE: This paper examined the extent to which 279 items reported in an I-PROM (PSYCHLOPS) added qualitative information which was not captured by two well-established outcome measures (CORE-OM and PHQ-9)...
November 22, 2017: Health Expectations: An International Journal of Public Participation in Health Care and Health Policy
https://www.readbyqxmd.com/read/29160392/prevalence-and-sensitivity-of-bacilli-and-pseudomonas-in-the-newborn-s-oral-cavity
#16
Priscila Vitor Alves Ferreira, Isabela Amêndola, Luciane Dias de Oliveira, Célia Regina Gonçalves E Silva, Mariella Vieira Pereira Leão, Silvana Soléo Ferreira Dos Santos
The aim of this study was to isolate Enterobacteria and Pseudomonas from the oral cavity of hospitalized newborns (NB) and determine their prevalence and the sensitivity profile to most commonly used antibiotics for this age group. Samples from the oral cavity of NB from 24 to 48 h age were collected using swabs. The samples were inoculated on MacConkey agar, incubated and the colonies counted and identified. For each strain, the minimum inhibitory concentration (MIC) was determined using agar dilution test...
July 2017: Brazilian Dental Journal
https://www.readbyqxmd.com/read/29150273/recurrent-and-disseminated-pityriasis-versicolor-a-novel-clinical-form-consequent-to-malassezia-host-interaction
#17
Karina Romero-Sandoval, Anderson Alves Costa, Maria Glória Teixeira Sousa, Celia Regina Furucho, Neusa Valente, Paulo R Criado, Valeria Aoki, Gil Benard
Pityriasis versicolor is a superficial fungal infection caused by Malassezia spp. The aim of this study is to propose the definition of a new clinical entity: the recurrent and disseminated pityriasis versicolor (RDPV). All patients with RDPV were enrolled over an eight-month period. Clinical and epidemiological data were obtained, Malassezia (M.) species were isolated in cultures and identified by phenotypic and molecular characterization, skin biopsies were taken from active lesions, serum levels of immunoglobulin E were obtained and therapeutic schemes were evaluated...
November 2017: Medical Hypotheses
https://www.readbyqxmd.com/read/29094611/the-carboxylic-acid-transporters-jen1-and-jen2-affect-the-architecture-and-fluconazole-susceptibility-of-candida-albicans-biofilm-in-the-presence-of-lactate
#18
Rosana Alves, Sandra Mota, Sónia Silva, Célia F Rodrigues, Alistair J P Brown, Mariana Henriques, Margarida Casal, Sandra Paiva
Candida albicans has the ability to adapt to different host niches, often glucose-limited but rich in alternative carbon sources. In these glucose-poor microenvironments, this pathogen expresses JEN1 and JEN2 genes, encoding carboxylate transporters, which are important in the early stages of infection. This work investigated how host microenvironments, in particular acidic containing lactic acid, affect C. albicans biofilm formation and antifungal drug resistance. Multiple components of the extracellular matrix were also analysed, including their impact on antifungal drug resistance, and the involvement of both Jen1 and Jen2 in this process...
November 2017: Biofouling
https://www.readbyqxmd.com/read/28941699/synthesis-of-whey-peptide-iron-complexes-influence-of-using-different-iron-precursor-compounds
#19
Maria Elisa Caetano-Silva, Renata Carolina Alves, Guilherme Nunes Lucena, Regina Célia Galvão Frem, Maria Teresa Bertoldo-Pacheco, Juliana Azevedo Lima-Pallone, Flavia Maria Netto
Iron-binding peptides are an alternative for increasing the bioavailability of iron and to decreasing its pro-oxidant effect. This study aimed to synthesize and characterize peptide-iron complexes using FeCl2 or FeSO4 as the iron precursor compounds. Whey protein isolate (WPI), WPI hydrolyzed with pancreatin, and its fractions obtained via ultrafiltration (cut-off 5kDa) were used as ligands. The fluorescence intensity of the ligands significantly decreased as the iron concentration increased as a result of metal coordination with the iron-binding sites, which may have led to changes in the microenvironment of tryptophan...
November 2017: Food Research International
https://www.readbyqxmd.com/read/28917322/high-genetic-diversity-in-toxoplasma-gondii-isolates-from-pigs-at-slaughterhouses-in-para%C3%A3-ba-state-northeastern-brazil-circulation-of-new-genotypes-and-brazilian-clonal-lineages
#20
Thais Ferreira Feitosa, Vinícius Longo Ribeiro Vilela, João Leite de Almeida-Neto, Antonielson Dos Santos, Dayana Firmino de Morais, Bruna Farias Alves, Fabiana Nakashima, Solange Maria Gennari, Ana Célia Rodrigues Athayde, Hilda Fátima Jesus Pena
The consumption of raw or undercooked pig meat containing Toxoplasma gondii cysts is an important transmission route of this protozoon to animals and humans. This study aimed to serologically diagnose, isolate and genotype T. gondii from pigs slaughtered for human consumption in the state of Paraíba, northeastern Brazil. Blood and tissue samples (heart, tongue and brain) were collected from 120 pigs at slaughterhouses in the state of Paraíba. Serological examinations were performed with an indirect immunofluorescent antibody test (IFAT) with a cut-off point of 1:64...
September 15, 2017: Veterinary Parasitology
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