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https://www.readbyqxmd.com/read/29625021/hepatitis-c-viral-load-in-hcv-monoinfected-and-hcv-hiv-1-hcv-htlv-1-2-and-hcv-hiv-htlv-1-2-co-infected-patients-from-s%C3%A3-o-paulo-brazil
#1
Fabiana Aparecida Alves, Karoline Rodrigues Campos, Marcílio Figueiredo Lemos, Regina Célia Moreira, Adele Caterino-de-Araujo
Co-infections of hepatitis C virus (HCV) and either human immunodeficiency virus type 1 (HIV-1), human T-cell lymphotropic virus type 1 (HTLV-1) or type 2 (HTLV-2) have been described as having an impact on HCV viremia and subsequent disease progression. HCV load in serum samples from 622 patients (343 males, 279 females; median age 50.8 years) from São Paulo/southeast Brazil was analyzed using the Abbott Real Time HCV assay (Abbott Molecular Inc., IL, USA). Samples were obtained from HCV-monoinfected (n=548), HCV/HIV-1- (n=41), HCV/HTLV-1- (n=16), HCV/HTLV-2- (n=8), HCV/HIV/HTLV-1- (n=4), and HCV/HIV/HTLV-2-co-infected (n=5) patients, and results were compared among the groups and according to sex...
April 3, 2018: Brazilian Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29546667/proteomic-analysis-of-a-549-cells-infected-with-human-adenovirus-40-by-lc-ms
#2
Ana Carla Peixoto Guissoni, Célia Maria Almeida Soares, Kareem R Badr, Fabiola Sousa Ficcadori, Ana Flávia Alves Parente, Juliana Alves Parente, Lilian Cristina Baeza, Menira Souza, Divina das Dores de Paula Cardoso
Human Adenoviruses (HAdVs) are etiological agents of different syndromes such as gastroenteritis, cystitis, ocular, and respiratory diseases, and infection by these viruses may cause alterations in cellular homeostasis. The objective of the study was the proteomic analysis of A-549 cells infected with HAdV-40 using LC-MS. At 30 h of infection, the quantitative analysis revealed 336 differentially expressed proteins. From them, 206 were induced (up-regulated) and 130 were suppressed (down-regulated). The majority of up-regulated proteins were related to energy, cellular organization, stress response, and apoptosis pathways...
March 15, 2018: Virus Genes
https://www.readbyqxmd.com/read/29526166/distinct-genetic-profiles-of-leishmania-viannia-braziliensis-associate-with-clinical-variations-in-cutaneous-leishmaniasis-patients-from-an-endemic-area-in-brazil
#3
Patrícia Flávia Quaresma, Cristiana Ferreira Alves de Brito, Jeronimo Marteleto Nunes Rugani, Janaína de Moura Freire, Rodrigo de Paula Baptista, Elizabeth Castro Moreno, Raquel Carvalho Gontijo, Felipe Dutra Rego, Joaquim Edemilson Diniz, Maria Norma Melo, Célia Maria Ferreira Gontijo
American tegumentary leishmaniasis (ATL) samples obtained from the lesions of patients with typical (n = 25, 29%), atypical (n = 60, 69%) or both (n = 2%) clinical manifestations were analysed by multilocus enzyme electrophoresis, hsp70 restriction-fragment length polymorphism (PCR-RFLP), hsp70 sequencing and phylogenetics methods. The hsp70 PCR-RFLP analysis revealed two different profiles whose the most samples differed from those expected for Leishmania braziliensis and the other Leishmania species tested: of 39 samples evaluated, two (5%) had a restriction profile corresponding to L...
March 12, 2018: Parasitology
https://www.readbyqxmd.com/read/29499552/corrigendum-to-does-outcome-measurement-of-treatment-for-substance-use-disorder-reflect-the-personal-concerns-of-patients-a-scoping-review-of-measures-recommended-in-europe-drug-alcohol-dependence-179-2017-299-308
#4
https://www.readbyqxmd.com/read/29407415/waardenburg-syndrome-novel-mutations-in-a-large-brazilian-sample
#5
Magnolia Astrid Pretell Bocángel, Uirá Souto Melo, Leandro Ucela Alves, Eliete Pardono, Naila Cristina Vilaça Lourenço, Humberto Vicente Cezar Marcolino, Paulo Alberto Otto, Regina Célia Mingroni-Netto
This paper deals with the molecular investigation of Waardenburg syndrome (WS) in a sample of 49 clinically diagnosed probands (most from southeastern Brazil), 24 of them having the type 1 (WS1) variant (10 familial and 14 isolated cases) and 25 being affected by the type 2 (WS2) variant (five familial and 20 isolated cases). Sequential Sanger sequencing of all coding exons of PAX3, MITF, EDN3, EDNRB, SOX10 and SNAI2 genes, followed by CNV detection by MLPA of PAX3, MITF and SOX10 genes in selected cases revealed many novel pathogenic variants...
January 30, 2018: European Journal of Medical Genetics
https://www.readbyqxmd.com/read/29236927/making-the-invisible-visible-searching-for-human-t-cell-lymphotropic-virus-types-1-and-2-htlv-1-and-htlv-2-in-brazilian-patients-with-viral-hepatitis-b-and-c
#6
Adele Caterino-de-Araujo, Fabiana Aparecida Alves, Karoline Rodrigues Campos, Marcílio Figueiredo Lemos, Regina Célia Moreira
With this study, the authors hope to alert clinicians regarding the presence of human T-cell lymphotropic virus type 1 and 2 (HTLV-1/-2) infections in patients with viral hepatitis B and C in Brazil. HTLV-1/-2 were detected in 1.3% of hepatitis B virus (HBV)- and 5.3% of hepatitis C virus (HCV)-infected blood samples sent for laboratory viral load measurements. A partial association of human immunodeficiency virus (HIV)-1 and HTLV-1/-2 infection was detected in patients with HCV (HIV+, 27.3%), whereas this association was almost 100% in HBV-infected patients (HIV+, all except one)...
February 2018: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/29211181/-violence-committed-by-acquaintances-brazil-2013
#7
Márcio Dênis Medeiros Mascarenhas, Raniela Borges Sinimbu, Deborah Carvalho Malta, Marta Maria Alves da Silva, Alexandre Fonseca Santos, Maria Lucia França Pontes Vieira, Célia Landmann Szwarcwald
The scope of this article is to describe the prevalence of violence committed by acquaintances in accordance with demographic characteristics. It is a descriptive study, based on data from a population-based National Health Research survey conducted in Brazil in 2013. Data from the adult population (≥ 18 years) of 64,348 households. Prevalence rates were calculated and their respective 95% confidence intervals (95%CI) according to gender, age, education, race/color, area of residence and geographical region...
November 2017: Ciência & Saúde Coletiva
https://www.readbyqxmd.com/read/29189583/modeling-fitness-variable-responses-to-training-in-prepubescent-children
#8
Ana R Alves, Carlos Marta, Henrique P Neiva, Célia Nunes, Mikel Izquierdo, Mário C Marques
The aim of this study was to determine strength and oxygen uptake (VO2max) performances according to different training program intervention design with 8-weeks duration in prepubescent children through a multiple linear regression models. Two hundred and forty-five healthy prepubescent children (aged 10.9 ± 0.5 years) were randomly assigned to a specific training program (strength training only - S; aerobic training only - A; intra-session aerobic and strength training - AS; intra-session strength and aerobic training - SA; or concurrent training performed in different sessions - CT) or a control group (no training regimen - C)...
November 16, 2017: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/29165852/capturing-and-missing-the-patient-s-story-through-outcome-measures-a-thematic-comparison-of-patient-generated-items-in-psychlops-with-core-om-and-phq-9
#9
Célia Md Sales, Inês Td Neves, Paula G Alves, Mark Ashworth
BACKGROUND: There is increasing interest in individualized patient-reported outcome measures (I-PROMS), where patients themselves indicate the specific problems they want to address in therapy and these problems are used as items within the outcome measurement tool. OBJECTIVE: This paper examined the extent to which 279 items reported in an I-PROM (PSYCHLOPS) added qualitative information which was not captured by two well-established outcome measures (CORE-OM and PHQ-9)...
November 22, 2017: Health Expectations: An International Journal of Public Participation in Health Care and Health Policy
https://www.readbyqxmd.com/read/29160392/prevalence-and-sensitivity-of-bacilli-and-pseudomonas-in-the-newborn-s-oral-cavity
#10
Priscila Vitor Alves Ferreira, Isabela Amêndola, Luciane Dias de Oliveira, Célia Regina Gonçalves E Silva, Mariella Vieira Pereira Leão, Silvana Soléo Ferreira Dos Santos
The aim of this study was to isolate Enterobacteria and Pseudomonas from the oral cavity of hospitalized newborns (NB) and determine their prevalence and the sensitivity profile to most commonly used antibiotics for this age group. Samples from the oral cavity of NB from 24 to 48 h age were collected using swabs. The samples were inoculated on MacConkey agar, incubated and the colonies counted and identified. For each strain, the minimum inhibitory concentration (MIC) was determined using agar dilution test...
July 2017: Brazilian Dental Journal
https://www.readbyqxmd.com/read/29150273/recurrent-and-disseminated-pityriasis-versicolor-a-novel-clinical-form-consequent-to-malassezia-host-interaction
#11
Karina Romero-Sandoval, Anderson Alves Costa, Maria Glória Teixeira Sousa, Celia Regina Furucho, Neusa Valente, Paulo R Criado, Valeria Aoki, Gil Benard
Pityriasis versicolor is a superficial fungal infection caused by Malassezia spp. The aim of this study is to propose the definition of a new clinical entity: the recurrent and disseminated pityriasis versicolor (RDPV). All patients with RDPV were enrolled over an eight-month period. Clinical and epidemiological data were obtained, Malassezia (M.) species were isolated in cultures and identified by phenotypic and molecular characterization, skin biopsies were taken from active lesions, serum levels of immunoglobulin E were obtained and therapeutic schemes were evaluated...
November 2017: Medical Hypotheses
https://www.readbyqxmd.com/read/29094611/the-carboxylic-acid-transporters-jen1-and-jen2-affect-the-architecture-and-fluconazole-susceptibility-of-candida-albicans-biofilm-in-the-presence-of-lactate
#12
Rosana Alves, Sandra Mota, Sónia Silva, Célia F Rodrigues, Alistair J P Brown, Mariana Henriques, Margarida Casal, Sandra Paiva
Candida albicans has the ability to adapt to different host niches, often glucose-limited but rich in alternative carbon sources. In these glucose-poor microenvironments, this pathogen expresses JEN1 and JEN2 genes, encoding carboxylate transporters, which are important in the early stages of infection. This work investigated how host microenvironments, in particular acidic containing lactic acid, affect C. albicans biofilm formation and antifungal drug resistance. Multiple components of the extracellular matrix were also analysed, including their impact on antifungal drug resistance, and the involvement of both Jen1 and Jen2 in this process...
November 2017: Biofouling
https://www.readbyqxmd.com/read/28941699/synthesis-of-whey-peptide-iron-complexes-influence-of-using-different-iron-precursor-compounds
#13
Maria Elisa Caetano-Silva, Renata Carolina Alves, Guilherme Nunes Lucena, Regina Célia Galvão Frem, Maria Teresa Bertoldo-Pacheco, Juliana Azevedo Lima-Pallone, Flavia Maria Netto
Iron-binding peptides are an alternative for increasing the bioavailability of iron and to decreasing its pro-oxidant effect. This study aimed to synthesize and characterize peptide-iron complexes using FeCl2 or FeSO4 as the iron precursor compounds. Whey protein isolate (WPI), WPI hydrolyzed with pancreatin, and its fractions obtained via ultrafiltration (cut-off 5kDa) were used as ligands. The fluorescence intensity of the ligands significantly decreased as the iron concentration increased as a result of metal coordination with the iron-binding sites, which may have led to changes in the microenvironment of tryptophan...
November 2017: Food Research International
https://www.readbyqxmd.com/read/28917322/high-genetic-diversity-in-toxoplasma-gondii-isolates-from-pigs-at-slaughterhouses-in-para%C3%A3-ba-state-northeastern-brazil-circulation-of-new-genotypes-and-brazilian-clonal-lineages
#14
Thais Ferreira Feitosa, Vinícius Longo Ribeiro Vilela, João Leite de Almeida-Neto, Antonielson Dos Santos, Dayana Firmino de Morais, Bruna Farias Alves, Fabiana Nakashima, Solange Maria Gennari, Ana Célia Rodrigues Athayde, Hilda Fátima Jesus Pena
The consumption of raw or undercooked pig meat containing Toxoplasma gondii cysts is an important transmission route of this protozoon to animals and humans. This study aimed to serologically diagnose, isolate and genotype T. gondii from pigs slaughtered for human consumption in the state of Paraíba, northeastern Brazil. Blood and tissue samples (heart, tongue and brain) were collected from 120 pigs at slaughterhouses in the state of Paraíba. Serological examinations were performed with an indirect immunofluorescent antibody test (IFAT) with a cut-off point of 1:64...
September 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28844734/employing-proteomic-analysis-to-compare-paracoccidioides-lutzii-yeast-and-mycelium-cell-wall-proteins
#15
COMPARATIVE STUDY
Danielle Silva Araújo, Patrícia de Sousa Lima, Lilian Cristiane Baeza, Ana Flávia Alves Parente, Alexandre Melo Bailão, Clayton Luiz Borges, Célia Maria de Almeida Soares
Paracoccidioidomycosis is an important systemic mycosis caused by thermodimorphic fungi of the Paracoccidioides genus. During the infective process, the cell wall acts at the interface between the fungus and the host. In this way, the cell wall has a key role in growth, environment sensing and interaction, as well as morphogenesis of the fungus. Since the cell wall is absent in mammals, it may present molecules that are described as target sites for new antifungal drugs. Despite its importance, up to now few studies have been conducted employing proteomics in for the identification of cell wall proteins in Paracoccidioides spp...
November 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28830035/does-outcome-measurement-of-treatment-for-substance-use-disorder-reflect-the-personal-concerns-of-patients-a-scoping-review-of-measures-recommended-in-europe
#16
REVIEW
Paula Alves, Célia Sales, Mark Ashworth
There are a growing number of authors stating that outcome measurement in treatment for substance use disorders should go beyond substance use and include other bio-psycho-social variables of interest. However, little is known about which topics tend to be covered by outcome measures and whether they reflect the typical concerns of this patient group. This study followed a scoping review methodology in which 42 outcome measures recommended by an EU agency for substance use disorders were reviewed. We identified the domains of problems covered by these 42 measures and then compared them with 54 domains derived from patients, in a previous study...
July 28, 2017: Drug and Alcohol Dependence
https://www.readbyqxmd.com/read/28767922/pemphigus-foliaceus-as-a-differential-diagnosis-in-vesicobullous-lesions
#17
Louise de Almeida Ferreira Fonseca, Célia Antônia Xavier de Moraes Alves, Ivan Aprahamian, Clóvis Antônio Lopes Pinto
Given the challenge of clinical diagnosis of bullous skin lesions, this report aimed to discuss the histological changes, the presentation and clinical reasoning for diagnosis of these lesions. At the same time, the importance of the pathology was reviewed to identify these clinical scenarios. In this case report, we highlighted the clinical progression of a case of pemphigus foliaceus. RESUMO Considerando o desafio do diagnóstico clínico de lesões cutâneas de apresentação bolhosa, o presente trabalho procurou discutir as alterações histológicas, a apresentação e o raciocínio clínico para o diagnóstico de tais lesões...
April 2017: Einstein
https://www.readbyqxmd.com/read/28724004/international-cooperation-and-shortage-of-doctors-an-analysis-of-the-interaction-between-brazil-angola-and-cuba
#18
Sandra Mara Campos Alves, Felipe Proenço de Oliveira, Mateus Falcão Martins Matos, Leonor Maria Pacheco Santos, Maria Celia Delduque
The shortage of doctors, especially in remote areas, is a critical issue for the development of national health systems and has thus been the focus of a number of international cooperation projects. An exploratory and qualitative study was conducted to examine cooperation between Brazil, Angola and Cuba. A nonsystematic literature review was conducted of selected open access articles and official documents addressing relevant health cooperation initiatives. Previously selected characteristics of actions designed to redress the shortage of doctors were compared...
July 2017: Ciência & Saúde Coletiva
https://www.readbyqxmd.com/read/28704618/paracoccidioides-brasiliensis-presents-metabolic-reprogramming-and-secretes-a-serine-proteinase-during-murine-infection
#19
Laurine Lacerda Pigosso, Lilian Cristiane Baeza, Mariana Vieira Tomazett, Mariana Batista Rodrigues Faleiro, Veridiana Maria Brianezi Dignani de Moura, Alexandre Melo Bailão, Clayton Luiz Borges, Juliana Alves Parente Rocha, Gabriel Rocha Fernandes, Gregory M Gauthier, Celia Maria de Almeida Soares
Paracoccidoides brasiliensis and Paracoccidioides lutzii, the etiologic agents of paracoccidioidomycosis, cause disease in healthy and immunocompromised persons in Latin America. We developed a method for harvesting P. brasiliensis yeast cells from infected murine lung to facilitate in vivo transcriptional and proteomic profiling. P. brasiliensis harvested at 6 h post-infection were analyzed using RNAseq and LC-MSE . In vivo yeast cells had 594 differentially expressed transcripts and 350 differentially expressed proteins...
October 3, 2017: Virulence
https://www.readbyqxmd.com/read/28638980/first-report-of-typical-brazilian-toxoplasma-gondii-genotypes-from-isolates-of-free-range-chickens-gallus-gallus-domesticus-circulating-in-the-state-of-para%C3%A3-ba-northeast-brazil
#20
Thais Ferreira Feitosa, Vinícius Longo Ribeiro Vilela, João Leite de Almeida-Neto, Lídio Ricardo Bezerra de Melo, Dayana Firmino de Morais, Bruna Farias Alves, Fabiana Nakashima, Solange Maria Gennari, Ana Célia Rodrigues Athayde, Hilda Fátima de Jesus Pena
This study evaluated, for the first time, the genetic diversity of Toxoplasma gondii isolates from free-range chickens from the state of Paraíba, Northeast Brazil. Tissue samples from 33 chickens from properties in five municipalities of Paraíba (Esperança, Olho d'Água, Malta, Monteiro, and Patos) were bioassayed in mice. The brains of mice infected with T. gondii cysts were used for DNA extraction and genotyping. Genotyping was performed using 11 PCR-RFLP markers and 15 microsatellite (MS) markers. Complete genotyping results were obtained for 29 isolates, with nine genotypes detected by RFLP and 15 genotypes identified by MS...
August 2017: Parasitology Research
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