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https://www.readbyqxmd.com/read/28941699/synthesis-of-whey-peptide-iron-complexes-influence-of-using-different-iron-precursor-compounds
#1
Maria Elisa Caetano-Silva, Renata Carolina Alves, Guilherme Nunes Lucena, Regina Célia Galvão Frem, Maria Teresa Bertoldo-Pacheco, Juliana Azevedo Lima-Pallone, Flavia Maria Netto
Iron-binding peptides are an alternative for increasing the bioavailability of iron and to decreasing its pro-oxidant effect. This study aimed to synthesize and characterize peptide-iron complexes using FeCl2 or FeSO4 as the iron precursor compounds. Whey protein isolate (WPI), WPI hydrolyzed with pancreatin, and its fractions obtained via ultrafiltration (cut-off 5kDa) were used as ligands. The fluorescence intensity of the ligands significantly decreased as the iron concentration increased as a result of metal coordination with the iron-binding sites, which may have led to changes in the microenvironment of tryptophan...
November 2017: Food Research International
https://www.readbyqxmd.com/read/28917322/high-genetic-diversity-in-toxoplasma-gondii-isolates-from-pigs-at-slaughterhouses-in-para%C3%A3-ba-state-northeastern-brazil-circulation-of-new-genotypes-and-brazilian-clonal-lineages
#2
Thais Ferreira Feitosa, Vinícius Longo Ribeiro Vilela, João Leite de Almeida-Neto, Antonielson Dos Santos, Dayana Firmino de Morais, Bruna Farias Alves, Fabiana Nakashima, Solange Maria Gennari, Ana Célia Rodrigues Athayde, Hilda Fátima Jesus Pena
The consumption of raw or undercooked pig meat containing Toxoplasma gondii cysts is an important transmission route of this protozoon to animals and humans. This study aimed to serologically diagnose, isolate and genotype T. gondii from pigs slaughtered for human consumption in the state of Paraíba, northeastern Brazil. Blood and tissue samples (heart, tongue and brain) were collected from 120 pigs at slaughterhouses in the state of Paraíba. Serological examinations were performed with an indirect immunofluorescent antibody test (IFAT) with a cut-off point of 1:64...
September 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28844734/employing-proteomic-analysis-to-compare-paracoccidioides-lutzii-yeast-and-mycelium-cell-wall-proteins
#3
Danielle Silva Araújo, Patrícia de Sousa Lima, Lilian Cristiane Baeza, Ana Flávia Alves Parente, Alexandre Melo Bailão, Clayton Luiz Borges, Célia Maria de Almeida Soares
Paracoccidioidomycosis is an important systemic mycosis caused by thermodimorphic fungi of the Paracoccidioides genus. During the infective process, the cell wall acts at the interface between the fungus and the host. In this way, the cell wall has a key role in growth, environment sensing and interaction, as well as morphogenesis of the fungus. Since the cell wall is absent in mammals, it may present molecules that are described as target sites for new antifungal drugs. Despite its importance, up to now few studies have been conducted employing proteomics in for the identification of cell wall proteins in Paracoccidioides spp...
August 24, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28830035/does-outcome-measurement-of-treatment-for-substance-use-disorder-reflect-the-personal-concerns-of-patients-a-scoping-review-of-measures-recommended-in-europe
#4
REVIEW
Paula Alves, Célia Sales, Mark Ashworth
There are a growing number of authors stating that outcome measurement in treatment for substance use disorders should go beyond substance use and include other bio-psycho-social variables of interest. However, little is known about which topics tend to be covered by outcome measures and whether they reflect the typical concerns of this patient group. This study followed a scoping review methodology in which 42 outcome measures recommended by an EU agency for substance use disorders were reviewed. We identified the domains of problems covered by these 42 measures and then compared them with 54 domains derived from patients, in a previous study...
July 28, 2017: Drug and Alcohol Dependence
https://www.readbyqxmd.com/read/28767922/pemphigus-foliaceus-as-a-differential-diagnosis-in-vesicobullous-lesions
#5
Louise de Almeida Ferreira Fonseca, Célia Antônia Xavier de Moraes Alves, Ivan Aprahamian, Clóvis Antônio Lopes Pinto
Given the challenge of clinical diagnosis of bullous skin lesions, this report aimed to discuss the histological changes, the presentation and clinical reasoning for diagnosis of these lesions. At the same time, the importance of the pathology was reviewed to identify these clinical scenarios. In this case report, we highlighted the clinical progression of a case of pemphigus foliaceus. RESUMO Considerando o desafio do diagnóstico clínico de lesões cutâneas de apresentação bolhosa, o presente trabalho procurou discutir as alterações histológicas, a apresentação e o raciocínio clínico para o diagnóstico de tais lesões...
April 2017: Einstein
https://www.readbyqxmd.com/read/28724004/international-cooperation-and-shortage-of-doctors-an-analysis-of-the-interaction-between-brazil-angola-and-cuba
#6
Sandra Mara Campos Alves, Felipe Proenço de Oliveira, Mateus Falcão Martins Matos, Leonor Maria Pacheco Santos, Maria Celia Delduque
The shortage of doctors, especially in remote areas, is a critical issue for the development of national health systems and has thus been the focus of a number of international cooperation projects. An exploratory and qualitative study was conducted to examine cooperation between Brazil, Angola and Cuba. A nonsystematic literature review was conducted of selected open access articles and official documents addressing relevant health cooperation initiatives. Previously selected characteristics of actions designed to redress the shortage of doctors were compared...
July 2017: Ciência & Saúde Coletiva
https://www.readbyqxmd.com/read/28704618/paracoccidioides-brasiliensis-presents-metabolic-reprogramming-and-secretes-a-serine-proteinase-during-murine-infection
#7
Laurine Lacerda Pigosso, Lilian Cristiane Baeza, Mariana Vieira Tomazett, Mariana Batista Rodrigues Faleiro, Veridiana Maria Brianezi Dignani de Moura, Alexandre Melo Bailão, Clayton Luiz Borges, Juliana Alves Parente Rocha, Gabriel Rocha Fernandes, Gregory M Gauthier, Celia Maria de Almeida Soares
Paracoccidoides brasiliensis and Paracoccidioides lutzii, the etiologic agents of paracoccidioidomycosis, cause disease in healthy and immunocompromised persons in Latin America. We developed a method for harvesting P. brasiliensis yeast cells from infected murine lung to facilitate in vivo transcriptional and proteomic profiling. P. brasiliensis harvested at 6 h post-infection were analyzed using RNAseq and LC-MS(E). In vivo yeast cells had 594 differentially expressed transcripts and 350 differentially expressed proteins...
July 13, 2017: Virulence
https://www.readbyqxmd.com/read/28638980/first-report-of-typical-brazilian-toxoplasma-gondii-genotypes-from-isolates-of-free-range-chickens-gallus-gallus-domesticus-circulating-in-the-state-of-para%C3%A3-ba-northeast-brazil
#8
Thais Ferreira Feitosa, Vinícius Longo Ribeiro Vilela, João Leite de Almeida-Neto, Lídio Ricardo Bezerra de Melo, Dayana Firmino de Morais, Bruna Farias Alves, Fabiana Nakashima, Solange Maria Gennari, Ana Célia Rodrigues Athayde, Hilda Fátima de Jesus Pena
This study evaluated, for the first time, the genetic diversity of Toxoplasma gondii isolates from free-range chickens from the state of Paraíba, Northeast Brazil. Tissue samples from 33 chickens from properties in five municipalities of Paraíba (Esperança, Olho d'Água, Malta, Monteiro, and Patos) were bioassayed in mice. The brains of mice infected with T. gondii cysts were used for DNA extraction and genotyping. Genotyping was performed using 11 PCR-RFLP markers and 15 microsatellite (MS) markers. Complete genotyping results were obtained for 29 isolates, with nine genotypes detected by RFLP and 15 genotypes identified by MS...
August 2017: Parasitology Research
https://www.readbyqxmd.com/read/28611248/the-aspergillus-fumigatus-crza-transcription-factor-activates-chitin-synthase-gene-expression-during-the-caspofungin-paradoxical-effect
#9
Laure Nicolas Annick Ries, Marina Campos Rocha, Patrícia Alves de Castro, Rafael Silva-Rocha, Roberto Nascimento Silva, Fernanda Zanolli Freitas, Leandro José de Assis, Maria Célia Bertolini, Iran Malavazi, Gustavo H Goldman
Aspergillus fumigatus is an opportunistic fungal pathogen that causes invasive aspergillosis (IA), a life-threatening disease in immunocompromised humans. The echinocandin caspofungin, adopted as a second-line therapy in combating IA, is a β-1,3-glucan synthase inhibitor, which, when used in high concentrations, reverts the anticipated A. fumigatus growth inhibition, a phenomenon called the "caspofungin paradoxical effect" (CPE). The CPE has been widely associated with increased chitin content in the cell wall due to a compensatory upregulation of chitin synthase-encoding genes...
June 13, 2017: MBio
https://www.readbyqxmd.com/read/28598366/isolation-of-monoterpene-dihydrochalcones-from-piper-montealegreanum-yuncker-piperaceae
#10
Harley da Silva Alves, Wilma Raianny Vieira da Rocha, Raimundo Braz-Filho, Maria Célia de Oliveira Chaves
Four new compounds were isolated from the branches of Piper montealegreanum Yuncker, a shrub found in the Amazon rainforest, including two new dihydrochalcones named claricine (1) and maisine (2), a cinnamic acid derivative 3 and a phenylalkanoid 4, along with a porphyrin identified as the known compound phaeophytin a (5). The structures were established using spectroscopic experiments, including 1D and 2D NMR and HRESIMS experiments, performed on the two monoterpene dihydrochalcones and their monoacetyl derivatives...
June 9, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28502193/early-onset-obesity-and-food-restriction-alter-hepatocyte-metabolism-in-adult-wistar-rats
#11
Nayra Thais D Branquinho, Gabriel Henrique P Cruz, Cristian L Borrasca, Lucas de Paula S Alves, Célia Regina de Godoy Gomes, Vilma Aparecida Ferreira de Godoi, Maria Montserrat Diaz Pedrosa
CONTEXT: Caloric restriction (CR) is suggested for overweight control. OBJECTIVE: Systemic and liver glucose metabolism in the reduced-litter (RL) rat model under 30% CR was investigated. MATERIALS AND METHODS: Newborn litters were organised in control (G9); RL with free diet (G3L); and RL with CR (G3R). Assessments were made at the age of 90 d. RESULTS: Higher liver glycogen content and changes in systemic glucose handling were found in the RL groups...
December 2017: Archives of Physiology and Biochemistry
https://www.readbyqxmd.com/read/28500551/effect-of-industrial-and-domestic-ash-from-biomass-combustion-and-spent-coffee-grounds-on-soil-fertility-and-plant-growth-experiments-at-field-conditions
#12
João Peres Ribeiro, Estela Domingos Vicente, Ana Paula Gomes, Maria Isabel Nunes, Célia Alves, Luís A C Tarelho
An experimental study was conducted at field conditions in order to evaluate the effect of application of ash from biomass combustion on some soil fertility characteristics and plant growth. Application of 7.5 Mg ha(-1) industrial fly ash (IA), domestic ash (DA), and a 50:50 mix of domestic ash (DA) and spent coffee grounds (SCG) was made in different soil parcels. Lolium perenne seeds were sown and the grown biomass was harvested and quantified after 60 days. Soil samples from each parcel were also collected after that period and characterized...
May 13, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28429268/mutagenicity-assessment-of-aerosols-in-emissions-from-domestic-combustion-processes
#13
EDITORIAL
Nuno Canha, Isabel Lopes, Estela Domingos Vicente, Ana M Vicente, Benjamin A Musa Bandowe, Susana Marta Almeida, Célia A Alves
No abstract text is available yet for this article.
May 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28403369/fear-of-falling-in-older-adults-living-at-home-associated-factors
#14
Luciano Magalhães Vitorino, Carla Araujo Bastos Teixeira, Eliandra Laís Vilas Boas, Rúbia Lopes Pereira, Naiana Oliveira Dos Santos, Célia Alves Rozendo
OBJECTIVE: To identify the factors associated with the fear of falling in the older adultliving at home. METHOD: Cross-sectional study with probabilistic sampling of older adultenrolled in two Family Health Strategies (FHS). The fear of falling was measured by the Brazilian version of the Falls Efficacy Scale-International and by a household questionnairethat contained the explanatory variables. Multiple Linear Regression using the stepwise selection technique and the Generalized Linear Models were used in the statistical analyses...
April 10, 2017: Revista da Escola de Enfermagem da U S P
https://www.readbyqxmd.com/read/28395264/polycyclic-aromatic-hydrocarbons-and-their-derivatives-nitro-pahs-oxygenated-pahs-and-azaarenes-in-pm2-5-from-southern-european-cities
#15
Célia A Alves, Ana M Vicente, Danilo Custódio, Mário Cerqueira, Teresa Nunes, Casimiro Pio, Franco Lucarelli, Giulia Calzolai, Silvia Nava, Evangelia Diapouli, Konstantinos Eleftheriadis, Xavier Querol, Benjamin A Musa Bandowe
Atmospheric particulate matter (PM2.5) samples were collected over two one month periods during winter and summer in three Southern European cities (Oporto - traffic site, Florence - urban background, Athens - suburban). Concentrations of 27 polycyclic aromatic hydrocarbons (PAHs), 15 nitro-PAHs (NPAHs), 15 oxygenated-PAHs (OPAHs) and 4 azaarenes (AZAs) were determined. On average, the winter-summer concentrations of ΣPAHs were 16.3-5.60, 7.75-3.02 and 3.44-0.658ngm(-3) in Oporto, Florence and Athens, respectively...
October 1, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28372736/hydrocarbons-in-particulate-samples-from-wildfire-events-in-central-portugal-in-summer-2010
#16
Ana Vicente, Ana Calvo, Ana P Fernandes, Teresa Nunes, Cristina Monteiro, Casimiro Pio, Célia Alves
In summer 2010, twenty eight (14 PM2.5 samples plus 14 samples PM2.5-10) smoke samples were collected during wildfires that occurred in central Portugal. A portable high-volume sampler was used to perform the sampling, on quartz fibre filters of coarse (PM2.5-10) and fine (PM2.5) smoke samples. The carbonaceous content (elemental and organic carbon) of particulate matter was analysed by a thermal-optical technique. Subsequently, the particulate samples were solvent extracted and fractionated by vacuum flash chromatography into three different classes of organic compounds (aliphatics, polycyclic aromatic hydrocarbons (PAHs) and carbonyl compounds)...
March 2017: Journal of Environmental Sciences (China)
https://www.readbyqxmd.com/read/28327794/seasonality-of-sand-flies-diptera-psychodidae-and-leishmania-dna-detection-in-vector-species-in-an-area-with-endemic-visceral-leishmaniasis
#17
Lara Saraiva, Camila Gonçalves Leite, Ana Cristina Vianna Mariano da Rocha Lima, Luiz Otávio Alves de Carvalho, Agnes Antônia Sampaio Pereira, Jerônimo Marteleto Nunes Rugani, Felipe Dutra Rego, Célia Maria Ferreira Gontijo, José Dilermando Andrade
BACKGROUND: Leishmaniases are a serious health problem in southeast Brazil, including the city of Belo Horizonte (BH), Minas Gerais state (MG), where there are high rates of incidence and mortality due to visceral leishmaniases. BH is divided into nine sanitary districts (SD) of which one, the Venda Nova SD, was selected for this study because it has high rates of positivity for canine leishmaniasis and high incidence of human leishmaniasis. OBJECTIVES: This study aimed to survey the sand fly fauna in Venda Nova SD from August 2011 to July 2013 and perform a descriptive analysis of the vector population...
April 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28300892/scleromyxedema-clinical-diagnosis-and-autopsy-findings
#18
Ana Carolina Bulhões Sala, Paulo Rowilson Cunha, Clóvis Antônio Lopes Pinto, Célia Antônia Xavier de Moraes Alves, Ingrid Barreto Paiva, Ana Paula Vieira Araujo
Scleromyxedema is a rare chronic cutaneous mucinosis of unknown etiology. It is characterized by papular eruption and scleroderma with microscopic evidence of mucin deposition, fibroblast proliferation, and fibrosis. Most patients with scleromyxedema have monoclonal gammopathy and systemic manifestations resulting in significant morbidity and mortality. Several types of treatment have been reported with partial or inconsistent responses. Despite showing unpredictable evolution, systemic consequences of scleromyxedema and treatment side effects may result in death...
September 2016: Anais Brasileiros de Dermatologia
https://www.readbyqxmd.com/read/28294586/pediatric-eosinophilic-esophagitis-in-portugal
#19
J L Alves Marcelino, R Cardoso de Aguiar, F Cabral Duarte, A Célia Costa, M A Pereira-Barbosa
Eosinophilic esophagitis (EoE) is an increasingly frequent diagnosis in our clinical practice, mainly in pediatric age. Allergic responses to food and aeroallergens have been increasingly implicated in the etiology of this disease. We describe a retrospective data analysis of pediatric EoE patients followed in our Immunoallergology Department. Of the 25 children (22 male, average 10.8 years), 88% had prior history of rhinoconjunctivitis, 76% asthma, 48% eczema and 36% food allergy. After evaluation, we identified in 76% and 92% of patients food and aeroallergen sensitization, respectively; 68% had simultaneously food and inhalant sensitization and 96% had at least one positive test to aeroallergens or food allergens...
March 2017: European Annals of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28241038/spatial-clustering-and-local-risk-of-leprosy-in-s%C3%A3-o-paulo-brazil
#20
Antônio Carlos Vieira Ramos, Mellina Yamamura, Luiz Henrique Arroyo, Marcela Paschoal Popolin, Francisco Chiaravalloti Neto, Pedro Fredemir Palha, Severina Alice da Costa Uchoa, Flávia Meneguetti Pieri, Ione Carvalho Pinto, Regina Célia Fiorati, Ana Angélica Rêgo de Queiroz, Aylana de Souza Belchior, Danielle Talita Dos Santos, Maria Concebida da Cunha Garcia, Juliane de Almeida Crispim, Luana Seles Alves, Thaís Zamboni Berra, Ricardo Alexandre Arcêncio
BACKGROUND: Although the detection rate is decreasing, the proportion of new cases with WHO grade 2 disability (G2D) is increasing, creating concern among policy makers and the Brazilian government. This study aimed to identify spatial clustering of leprosy and classify high-risk areas in a major leprosy cluster using the SatScan method. METHODS: Data were obtained including all leprosy cases diagnosed between January 2006 and December 2013. In addition to the clinical variable, information was also gathered regarding the G2D of the patient at diagnosis and after treatment...
February 2017: PLoS Neglected Tropical Diseases
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