Li Wei, Yizhi Pan, Yu Guo, Yin Zhu, Haoran Jin, Yingying Gu, Chuanshuang Li, Yaqin Wang, Jingjing Lin, Yongping Chen, Chunhai Ke, Lanman Xu
BACKGROUND: Alcoholic liver disease (ALD) is exacerbated by disruptions in intestinal microecology and immune imbalances within the gut-liver axis. The present study assesses the therapeutic potential of combining Akkermansia muciniphila ( A. muciniphila ) with inosine in alleviating alcohol-induced liver injury. METHODS: Male C57BL/6 mice, subjected to a Lieber-DeCarli diet with 5% alcohol for 4 weeks, served as the alcoholic liver injury model. Various analyzes, including quantitative reverse transcription polymerase chain reaction (qRT-PCR), ELISA, immunochemistry, 16S rRNA gene sequencing, and flow cytometry, were employed to evaluate liver injury parameters, intestinal barrier function, microbiota composition, and immune responses...
2024: Frontiers in Microbiology