Read by QxMD icon Read

MTT antibiofilm assay

Karolina Niska, Narcyz Knap, Anna Kędzia, Maciej Jaskiewicz, Wojciech Kamysz, Iwona Inkielewicz-Stepniak
Objectives: In dentistry, silver nanoparticles (AgNPs) have drawn particular attention because of their wide antimicrobial activity spectrum. However, controversial information on AgNPs toxicity limited their use in oral infections. Therefore, the aim of the present study was to evaluate the antibacterial activities against a panel of oral pathogenic bacteria and bacterial biofilms together with potential cytotoxic effects on human gingival fibroblasts of 10 nm AgNPs: non-functionalized - uncapped (AgNPs-UC) as well as surface-functionalized with capping agent: lipoic acid (AgNPs-LA), polyethylene glycol (AgNPs-PEG) or tannic acid (AgNPs-TA) using silver nitrate (AgNO3) as control...
2016: International Journal of Medical Sciences
Mengjun Sun, Zichao Zhou, Jiachen Dong, Jichun Zhang, Yiru Xia, Rong Shu
Docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) are two major omega-3 polyunsaturated fatty acids (n-3 PUFAs) with antimicrobial properties. In this study, we evaluated the potential antibacterial and antibiofilm activities of DHA and EPA against two periodontal pathogens, Porphyromonas gingivalis (P. gingivalis) and Fusobacterium nucleatum (F. nucleatum). MTT assay showed that DHA and EPA still exhibited no cytotoxicity to human oral tissue cells when the concentration came to 100 μM and 200 μM, respectively...
October 2016: Microbial Pathogenesis
Ayaz Ahmed, Anum Khalid Khan, Ayaz Anwar, Syed Abid Ali, Muhammad Raza Shah
Klebsiella pneumoniae (K. pneumoniae) is one of the major pathogen associated with nosocomial infections, especially catheter associated urinary tract infections which involved biofilm formation. This study was designed to evaluate the antibiofilm efficacy of gold nanoparticle conjugated with chlorhexidine (Au-CHX) against K. pneumoniae isolates. Au-CHX was synthesized and analyzed for stability by using UV-Visible spectrophotometry, atomic force microscopy (AFM), fourier transform infrared spectroscopy (FT-IR) and electrospray ionization mass spectroscopy (ESI-MS)...
September 2016: Microbial Pathogenesis
Marzieh Aghazadeh, Abed Zahedi Bialvaei, Mohammad Aghazadeh, Fahimeh Kabiri, Negar Saliani, Mehdi Yousefi, Hosein Eslami, Hossein Samadi Kafil
BACKGROUND: Candidiasis is one of the most prevalent and important opportunistic fungal infections of the oral cavity caused by Candida yeast species like Candida albicans, C. glabrata, and C. krusei. In addition, several bacteria can cause oral infections. The inhibition of microbial biofilm is the best way to prevent oral infections. OBJECTIVES: The aim of the present study is to evaluate the antifungal, antimicrobial, and anti-biofilm properties of ginger (Zingiber officinale) extract against Candida species and some bacterial pathogens and the extract's effects on biofilm formation...
February 2016: Jundishapur Journal of Microbiology
Domagoj Kifer, Vedran Mužinić, Maja Šegvić Klarić
Staphylococcus aureus is one of the most commonly isolated microbes in chronic rhinosinusitis (CRS) that can be complicated due to the formation of a staphylococcal biofilm. In this study, we investigated antimicrobial efficacy of single mupirocin and three types of monoterpenes (thymol, menthol and 1,8-cineole) as well as mupirocin-monoterpene combinations against S. aureus ATCC 29213 and 5 methicilin-resistant S. aureus strains (MRSA) grown in planktonic and biofilm form. MIC against planktonic bacteria as well as minimum biofilm-eliminating concentrations (MBECs) and minimum biofilm inhibitory concentrations (MBICs) were determined by TTC and MTT reduction assay, respectively...
September 2016: Journal of Antibiotics
Sivalingam Manju, Balasubramanian Malaikozhundan, Sekar Vijayakumar, Sathappan Shanthi, Ameeramja Jaishabanu, Perumal Ekambaram, Baskaralingam Vaseeharan
This study reports the biological synthesis of gold nanoparticles using essential oil of Nigella sativa (NsEO-AuNPs). The synthesized NsEO-AuNPs were characterized by UV-visible spectra, X-ray diffraction (XRD), FTIR and Transmission electron microscopy (TEM). UV-vis spectra of NsEO-AuNPs showed strong absorption peak at 540 nm. The X-ray diffraction analysis revealed crystalline nature of nanoparticle with distinctive facets (111, 200, 220 and 311 planes) of NsEO-AuNPs. The FTIR spectra recorded peaks at 3388, 2842, 1685, 1607, 1391 and 1018 cm(-1)...
February 2016: Microbial Pathogenesis
O Tabbene, S Azaiez, A Di Grazia, I Karkouch, I Ben Slimene, S Elkahoui, M N Alfeddy, B Casciaro, V Luca, F Limam, M L Mangoni
AIMS: In this study, we evaluated the ability of the lipopeptide bacillomycin D and the antifungal drug amphotericin B as well as their combination, to inhibit Candida albicans biofilm formation and to accelerate keratinocyte cell migration. METHODS AND RESULTS: The antibiofilm activity of bacillomycin D and its combination with amphotericin B was carried out by crystal violet colorimetric method. Our results have shown that, when combined together at low concentrations nontoxic to mammalian cells, corresponding to 1/32 MIC (0·39 μg ml(-1) ) and 1/4 MIC (0·06 μg ml(-1) ) for bacillomycin D and amphotericin B, respectively, a clear antibiofilm activity is manifested (95% inhibition of biofilm formation) along with a clear inhibition of germ tube formation...
February 2016: Journal of Applied Microbiology
A Merghni, H Marzouki, H Hentati, M Aouni, M Mastouri
BACKGROUND: Laurus nobilis L. is an aromatic herb with relevant medicinal properties due to its important chemical composition and its potential therapeutic effects. In this study, we investigate the chemical composition, the antibacterial and the antibiofilms activities of Tunisian L. nobilis L. essential oils against clinical Staphylococcus aureus strains. METHODS: The chemical composition of L. nobilis L. essential oils was analysed by Gas Chromatography-Mass Spectrometry (GC-MS)...
December 4, 2015: Pathologie-biologie
Naif Abdullah Al-Dhabi, Mariadhas Valan Arasu, Thankappan Sarasam Rejiniemon
This study aimed to investigate the in vitro antibacterial, antifungal, antibiofilm, antioxidant, and anticancer properties of isosteviol isolated from endangered medicinal plant Pittosporum tetraspermum. Pure compound was obtained and characterized by column chromatography followed by (1)H NMR, (13)C NMR, IR, and mass spectral analysis. The antimicrobial activities of the compound were assessed by the broth microdilution method and the antioxidant properties were determined using reducing ability assay, DPPH scavenging assay, hydroxyl radical scavenging activity, and superoxide radical scavenging assay...
2015: Evidence-based Complementary and Alternative Medicine: ECAM
Alyne Rodrigues de Araujo, Patrick Veras Quelemes, Márcia Luana Gomes Perfeito, Luíza Ianny de Lima, Melka Coêlho Sá, Paulo Humberto Moreira Nunes, Graziella Anselmo Joanitti, Peter Eaton, Maria José Dos Santos Soares, José Roberto de Souza de Almeida Leite
BACKGROUND: The methicillin resistance of bacteria from the genus Staphylococcus and its ability to form biofilms are important factors in pathogenesis of these microorganisms. Thus, the search for new antimicrobials agents, especially from plants, has been intensified. In this context, Terminalia species have been the subject of research for many pharmacological activities. In this study we evaluated the antibacterial, antibiofilm and cytotoxic activities of the ethanol extract (EtE) from Terminalia fagifolia stem bark as well as that of three fractions of the extract (AqF, HaF and WSF)...
2015: Annals of Clinical Microbiology and Antimicrobials
Xin Zhu, Zhi Ma, Jiajun Wang, Shuli Chou, Anshan Shan
Here, we found that simple substitution of amino acids in the middle position of the hydrophobic face of an amphipathic peptide RI16 with tryptophan (T9W) considerably transformed into an antimicrobial peptide specifically targeting Pseudomonas aeruginosa. Minimal inhibitory concentration (MIC) results demonstrated that T9W had a strong and specifically antimicrobial activity against P. aeruginosa, including antibiotic-resistant strains, but was not active against Escherichia coli, Salmonella typhimurium, Staphylococcus aureus and Staphyfococcus epidermidis...
2014: PloS One
Laura Nunes Silva, Danielle da Silva Trentin, Karine Rigon Zimmer, Janine Treter, Clara Lia Costa Brandelli, Amanda Piccoli Frasson, Tiana Tasca, Alexandre Gomes da Silva, Márcia Vanusa da Silva, Alexandre José Macedo
CONTEXT: The local communities living in the Brazilian Caatinga biome have a significant body of traditional knowledge on a considerable number of medicinal plants used to heal several maladies. OBJECTIVE: Based on ethnopharmacological data, this study screened 23 aqueous plant extracts against two well-known models of biofilm-forming bacteria: Staphylococcus epidermidis and Pseudomonas aeruginosa. MATERIALS AND METHODS: Crystal violet assay and scanning electron microscopy (SEM) were used to evaluate the effect of extracts on biofilm formation and measurements of the absorbance at 600 nm to assess bacterial growth...
March 2015: Pharmaceutical Biology
Thankappan Sarasam Rejiniemon, Mariadhas Valan Arasu, Veeramuthu Duraipandiyan, Karuppiah Ponmurugan, Naif Abdullah Al-Dhabi, Selvaraj Arokiyaraj, Paul Agastian, Ki Choon Choi
BACKGROUND: Plant metabolites have wide applications and have the potential to cure different diseases caused by microorganisms. The aim of the study was to evaluate the antimicrobial, antibiofilm, cytotoxic, antifeedant and larvicidal properties of novel quinine isolated from Aegle marmelos (Linn.) Correa. METHODS: A compound was obtained by eluting the crude extract, using varying concentrations of the solvents by the chromatographic purification. Broth micro dilution method was used to assess the antimicrobial activity and anticancer study was evaluated using MTT assay...
2014: Annals of Clinical Microbiology and Antimicrobials
Ian P Olwoch, Oppel B W Greeff, Gisela Jooné, Vanessa Steenkamp
BACKGROUND: Pectinex Ultra SP-L (Pectinex) is a microbial-derived enzyme that is used in the food industry and that has been shown to inhibit bacterial biofilms. It has been suggested that Pectinex may be useful in the management of biofilm-related bacterial infections and therefore warrants further investigation in this regard. The aim of this study was to investigate the cytotoxicity of Pectinex on cervical adenocarcinoma cells (HeLa), lymphocytes and neutrophils. Cell viability and morphology were assessed using an in vitro spectrophotometric MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide) assay and polarization-optical transmitted light differential interference contrast microscopy...
2014: BMC Microbiology
E M Costa, S Silva, M R Costa, M Pereira, D A Campos, J Odila, A R Madureira, A Cardelle-Cobas, F K Tavaria, A S Rodrigues, M M Pintado
A previous study showed that a chitosan mouthwash would be a valid alternative to current mouthwashes as it demonstrated, in vitro, significantly higher antibiofilm activity than two commercial mouthwashes. As such, the aim of this work was to verify the safety of the developed product and to validate, in vivo, the biological activity ascertained in vitro. Chitosan mouthwash safety was evaluated through Ames, MTT and V79 chromosomal aberration assay while antimicrobial activity was evaluated through in vivo assays...
October 13, 2014: Carbohydrate Polymers
Angelo Luís, Luiza Breitenfeld, Susana Ferreira, Ana Paula Duarte, Fernanda Domingues
BACKGROUND: Hakea sericea Schrader is an invasive shrub in Portuguese forests. OBJECTIVE: The goal of this work was to evaluate the antimicrobial activity of H. sericea extracts against several strains of microorganisms, including the ability to inhibit the formation of biofilms. Additionally the cytotoxic properties of these extracts, against human cells, were assessed. MATERIALS AND METHODS: The antimicrobial activity of the methanolic extracts of H...
January 2014: Pharmacognosy Magazine
Hernandez-Delgadillo Rene, Martínez-Sanmiguel Juan José, Sánchez-Nájera Rosa Isela, Cabral-Romero Claudio
Typically, the onset of candidiasis is characterised by the appearance of a biofilm of Candida albicans, which is associated with several diseases including oral candidiasis in young and elderly people. The objective of this work was to investigate the in vitro fungicidal activity as well as the antibiofilm activity of ambroxol (AMB) against C. albicans growth. In the present investigation, the fungicidal activity of AMB was established using the cell viability 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay...
April 2014: Mycoses
Nimmy Augustine, A K Goel, K C Sivakumar, R Ajay Kumar, Sabu Thomas
Resveratrol, a phytochemical commonly found in the skin of grapes and berries, was tested for its biofilm inhibitory activity against Vibrio cholerae. Biofilm inhibition was assessed using crystal violet assay. MTT assay was performed to check the viability of the treated bacterial cells and the biofilm architecture was analysed using confocal laser scanning microscopy. The possible target of the compound was determined by docking analysis. Results showed that subinhibitory concentrations of the compound could significantly inhibit biofilm formation in V...
February 15, 2014: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
V Ramalingam, R Rajaram, C PremKumar, P Santhanam, P Dhinesh, S Vinothkumar, K Kaleshkumar
Pseudomonas aeruginosa (JQ989348) was isolated from deep sea water sample and used for synthesis of silver nanoparticles (AgNPs). AgNPs were confirmed by analyzing surface plasmon resonance using UV-visible spectrophotometer at 420 nm. Further scanning electron microscope analysis confirmed the range of particle size between 13 and 76 nm and XRD pattern authorizes the anisotropic crystalline nature of AgNPs. Fourier transform infrared spectrum endorsed the presence of high amount of proteins and other secondary metabolites in synthesized AgNPs influence the reduction process and stabilization of nanoparticles...
September 2014: Journal of Basic Microbiology
Rutger Matthes, Claudia Bender, Rabea Schlüter, Ina Koban, René Bussiahn, Stephan Reuter, Jürgen Lademann, Klaus-Dieter Weltmann, Axel Kramer
The treatment of infected wounds is one possible therapeutic aspect of plasma medicine. Chronic wounds are often associated with microbial biofilms which limit the efficacy of antiseptics. The present study investigates two different surface barrier discharges with air plasma to compare their efficacy against microbial biofilms with chlorhexidine digluconate solution (CHX) as representative of an important antibiofilm antiseptic. Pseudomonas aeruginosa SG81 and Staphylococcus epidermidis RP62A were cultivated on polycarbonate discs...
2013: PloS One
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"