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Live/dead baclight kit

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https://www.readbyqxmd.com/read/27764679/clotrimazole-and-econazole-inhibit-streptococcus-mutans-biofilm-and-virulence-in-vitro
#1
Wei Qiu, Biao Ren, Huanqin Dai, Lixin Zhang, Qiong Zhang, Xuedong Zhou, Yuqing Li
OBJECTIVE: The aim of this study was to determine the inhibitory effect of eight antifungal drugs on S. mutans growth, biofilm formation and virulence factors. METHODS: The actions of antifungal drugs on S. mutans were determined by recovery plates and survival kinetic curves. Biofilms were observed by scanning electron microscopy and the viable cells were recovered on BHI plates, meanwhile biofilms were stained by BacLight live/dead kit to investigate the biofilm viability...
October 13, 2016: Archives of Oral Biology
https://www.readbyqxmd.com/read/27293453/chamaecyparis-obtusa-suppresses-virulence-genes-in-streptococcus-mutans
#2
Eun-Hee Kim, Sun-Young Kang, Bog-Im Park, Young-Hoi Kim, Young-Rae Lee, Jin-Hee Hoe, Na-Young Choi, Ji-Young Ra, So-Youn An, Yong-Ouk You
Chamaecyparis obtusa (C. obtusa) is known to have antimicrobial effects and has been used as a medicinal plant and in forest bathing. This study aimed to evaluate the anticariogenic activity of essential oil of C. obtusa on Streptococcus mutans, which is one of the most important bacterial causes of dental caries and dental biofilm formation. Essential oil from C. obtusa was extracted, and its effect on bacterial growth, acid production, and biofilm formation was evaluated. C. obtusa essential oil exhibited concentration-dependent inhibition of bacterial growth over 0...
2016: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/26885910/-effects-of-different-fibre-diameter-of-polypyrrole-after-direct-current-stimulation-on-adhesion-and-proliferation-of-cells-and-bacteria
#3
Ying-ting Yang, Yan Wei, Bin Xia, Zhen-gao Wang
OBJECTIVE: To study the effects of cells'and bacteria's adhesion and proliferation on different fiber diameters of polypyrrole coating with electricity. METHODS: Titanium coated with polypyrrole was divided into no electrical stimulation and stimulation groups, each group had 30-60 nm, 70- 100 nm, 130-170 nm diameters of the fiber. MC3T3 cells and Staphylococcus aureus (S. aureus) were inoculated on different fiber diameters of polypyrrole coating with and without electric stimulation...
February 18, 2016: Beijing da Xue Xue Bao. Yi Xue Ban, Journal of Peking University. Health Sciences
https://www.readbyqxmd.com/read/26656002/propidium-monoazide-treatment-to-distinguish-between-live-and-dead-methanogens-in-pure-cultures-and-environmental-samples
#4
Janine Heise, Marcella Nega, Mashal Alawi, Dirk Wagner
In clinical trials investigating human health and in the analysis of microbial communities in cultures and natural environments, it is a substantial challenge to differentiate between living, potentially active communities and dead cells. The DNA-intercalating dye propidium monoazide (PMA) enables the selective masking of DNA from dead, membrane-compromised cells immediately before DNA extraction. In the present study, we evaluated for the first time a PMA treatment for methanogenic archaea in cultures and particle-rich environmental samples...
February 2016: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/26647939/viability-and-effects-on-bacterial-proteins-by-oral-rinses-with-hypochlorous-acid-as-active-ingredient
#5
Diana Marcela Castillo, Yormaris Castillo, Nathaly Andrea Delgadillo, Yineth Neuta, Johana Jola, Justo Leonardo Calderón, Gloria Inés Lafaurie
This study investigated the effect of hypochlorous acid (HOCl) rinses and chlorhexidine (CHX) on the bacterial viability of S. mutans, A. israelii, P. gingivalis, A. actinomycetemcomitans, E. corrodens, C. rectus, K. oxytoca, K. pneumoniae and E. cloacae. The percentage of live bacteria was tested by fluorescence method using Live/Dead kit(r) and BacLight (Molecular Probes(r)) and compared between groups by the Kruskal-Wallis and U Mann-Whitney tests with Bonferroni correction (p value<0.012). The effect of HOCl and CHX on total proteins of P...
October 2015: Brazilian Dental Journal
https://www.readbyqxmd.com/read/26639716/towards-effective-and-stable-probiotics
#6
D R Yarullina, L G Damshkaln, N L Bruslik, O A Konovalova, O N Ilinskaya, V I Lozinsky
BACKGROUND: Probiotics are live microorganisms, generally either lactobacilli or bifidobacteria, which when administered in adequate amounts confer a health benefit to the host [1]. Due to the growing evidence of health benefits associated with their use, probiotics are of increasing interest and represent now a significant growth area in the functional foods industry [2]. However, to be effective, orally administered probiotics should survive preparation of dosage forms and passage through acidic environment of the gastrointestinal tract (GIT)...
2015: International Journal of Risk & Safety in Medicine
https://www.readbyqxmd.com/read/26440735/inactivation-of-cronobacter-sakazakii-in-reconstituted-infant-formula-by-combination-of-thymoquinone-and-mild-heat
#7
C Shi, Z Jia, Y Chen, M Yang, X Liu, Y Sun, Z Zheng, X Zhang, K Song, L Cui, A B Baloch, X Xia
AIMS: The objective of this study was to determine the combined effect of thymoquinone (TQ) and mild heat on Cronobacter sakazakii in reconstituted infant formula. METHODS AND RESULTS: Reconstituted infant formula samples inoculated with a mixture of four C. sakazakii strains (approx. 6·5 log CFU ml(-1) ) were prepared with various concentrations of TQ (0, 5, 10, 20 and 30 mmol l(-1) ) and were heated to 45, 50 and 55°C for 0, 10, 20, 30, 60 and 120 min, and the surviving populations of C...
December 2015: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/26188278/repeated-exposures-to-blue-light-activated-eosin-y-enhance-inactivation-of-e-faecalis-biofilms-in-vitro
#8
Karlo Marinic, Daniel Manoil, Anna Filieri, John C Wataha, Jacques Schrenzel, Norbert Lange, Serge Bouillaguet
BACKGROUND: In dentistry, antibacterial photodynamic therapy (a-PDT) has shown promising results for inactivating bacterial biofilms causing carious, endodontic and periodontal diseases. In the current study, we assessed the ability of eosin Y exposed to 3 irradiation protocols at inactivating Enterococcus faecalis biofilms, in vitro. METHODS: E. faecalis biofilms formed on hydroxyapatite disks were incubated with eosin Y (10-80μM), then activated with blue light using different irradiation protocols...
September 2015: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/25936763/application-of-flow-cytometry-and-pma-qpcr-to-distinguish-between-membrane-intact-and-membrane-compromised-bacteria-cells-in-an-aquatic-milieu
#9
COMPARATIVE STUDY
Nicole Zacharias, Thomas Kistemann, Christiane Schreiber
The paper compares two methods of distinguishing between alive and dead cells by differentiation on the basis of their membrane structure: LIVE/DEAD flow cytometry and PMA-qPCR. LIVE/DEAD flow cytometry was established using the LIVE/DEAD(®) BacLight™ Bacterial Viability Kit with different ratios of Legionella pneumophila and Escherichia coli cells with intact and compromised membranes (heat treated). The PMA-qPCR method was tested and modified, and results were compared with those from LIVE/DEAD flow cytometry using L...
November 2015: International Journal of Hygiene and Environmental Health
https://www.readbyqxmd.com/read/25889778/comparative-three-dimensional-analysis-of-initial-biofilm-formation-on-three-orthodontic-bracket-materials
#10
COMPARATIVE STUDY
Marc Philipp Dittmer, Carolina Fuchslocher Hellemann, Sebastian Grade, Wieland Heuer, Meike Stiesch, Rainer Schwestka-Polly, Anton Phillip Demling
INTRODUCTION: The purpose of the present study was to investigate and compare early biofilm formation on biomaterials, which are being used in contemporary fixed orthodontic treatment. METHODS: This study comprised 10 healthy volunteers (5 females and 5 males) with a mean age of 27.3 +-3.7 years. Three slabs of different orthodontic materials (stainless steel, gold and ceramic) were placed in randomized order on a splint in the mandibular molar region. Splints were inserted intraorally for 48 h...
2015: Head & Face Medicine
https://www.readbyqxmd.com/read/25889626/testing-a-dual-fluorescence-assay-to-monitor-the-viability-of-filamentous-cyanobacteria
#11
Tylor J Johnson, Michael B Hildreth, Liping Gu, Ruanbao Zhou, William R Gibbons
Filamentous cyanobacteria are currently being engineered to produce long-chain organic compounds, including 3rd generation biofuels. Because of their filamentous morphology, standard methods to quantify viability (e.g., plate counts) are not possible. This study investigated a dual-fluorescence assay based upon the LIVE/DEAD® BacLight™ Bacterial Viability Kit to quantify the percent viability of filamentous cyanobacteria using a microplate reader in a high throughput 96-well plate format. The manufacturer's protocol calls for an optical density normalization step to equalize the numbers of viable and non-viable cells used to generate calibration curves...
June 2015: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/25842164/the-effect-of-uranium-on-bacterial-viability-and-cell-surface-morphology-using-atomic-force-microscopy-in-the-presence-of-bicarbonate-ions
#12
Paola Sepulveda-Medina, Yelena Katsenovich, Vishal Musaramthota, Michelle Lee, Brady Lee, Rupak Dua, Leonel Lagos
Past disposal practices at nuclear production facilities have led to the release of liquid waste into the environment creating multiple radionuclide plumes. Microorganisms are known for the ability to interact with radionuclides and impact their mobility in soils and sediments. Gram-positive Arthrobacter sp. are one of the most common bacterial groups in soils and are found in large numbers in subsurface environments contaminated with radionuclides. This study experimentally analyzed changes on the bacteria surface at the nanoscale level after uranium exposure and evaluated the effect of aqueous bicarbonate ions on U(VI) toxicity of a low uranium-tolerant Arthrobacter oxydans strain G968 by investigating changes in adhesion forces and cell dimensions via atomic force microscopy (AFM)...
June 2015: Research in Microbiology
https://www.readbyqxmd.com/read/25757698/observation-of-antibacterial-effect-of-biodegradable-polymeric-nanoparticles-on-staphylococcus-epidermidis-biofilm-using-fe-sem-with-an-ionic-liquid
#13
Chisato Takahashi, Noriko Ogawa, Yoshiaki Kawashima, Hiromitsu Yamamoto
We successfully visualized the antibacterial behavior of biodegradable polymeric nanoparticles (NPs) on a biofilm formed by Staphylococcus epidermidis using field emission scanning electron microscopy (FE-SEM). A hydrophilic ionic liquid, 1-butyl-3-methylimidazolium tetrafluoroborate ([BMIM][BF4]), was applied for observation using FE-SEM. The differences in adherence and penetration behavior of three types of NPs were revealed using this method and confocal laser scanning microscopy. Biodegradable poly(dl-lactide-co-glycolide) (PLGA) NPs were prepared by the emulsion solvent diffusion method...
June 2015: Microscopy
https://www.readbyqxmd.com/read/25734758/antibacterial-coatings-on-titanium-surfaces-a-comparison-study-between-in-vitro-single-species-and-multispecies-biofilm
#14
Maria Godoy-Gallardo, Zhejun Wang, Ya Shen, José M Manero, Francisco J Gil, Daniel Rodriguez, Markus Haapasalo
Dental plaque is a biofilm that causes dental caries, gingivitis, and periodontitis. Most of the studies in antibacterial coatings have been conducted by in vitro single-species biofilm formation, but oral biofilm involves more than 700 different bacterial species that are able to interact. Therefore, new studies are focused on in vitro multispecies biofilm models that mimic in vivo biofilms. The aim of the present work was to study different antibacterial coatings onto titanium surfaces and evaluate the in vitro antimicrobial properties of the surfaces on two different bacterial species and an oral biofilm...
March 18, 2015: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/25462759/comparison-of-methodologies-for-enumerating-and-detecting-the-viability-of-ascaris-eggs-in-sewage-sludge-by-standard-incubation-microscopy-the-baclight-live-dead-viability-assay-and-other-vital-dyes
#15
COMPARATIVE STUDY
Alaa Karkashan, Basma Khallaf, Jacqueline Morris, Nerida Thurbon, Duncan Rouch, Stephen R Smith, Margaret Deighton
The aim of this study was to evaluate the Live/Dead BacLight viability kit as a method for enumerating viable eggs of Ascaris suum in sewage sludge as a surrogate for the human roundworm. The number and viability status of eggs of A. suum were accurately measured directly in sewage sludge samples by the BacLight method, compared to the conventional incubation-microscopy procedure. BacLight stains were not toxic to A. suum eggs, in contrast to some conventional vital dyes which disrupted viable eggs. The method was effective for the direct examination of eggs in heavily contaminated samples or seeded sludge containing ∼200 eggs/g DS in sludge with 5% DS content...
January 1, 2015: Water Research
https://www.readbyqxmd.com/read/25381910/cellulose-production-and-cellulose-synthase-gene-detection-in-acetic-acid-bacteria
#16
Maria José Valera, Maria Jesús Torija, Albert Mas, Estibaliz Mateo
The ability of acetic acid bacteria (AAB) to produce cellulose has gained much industrial interest due to the physical and chemical characteristics of bacterial cellulose. The production of cellulose occurs in the presence of oxygen and in a glucose-containing medium, but it can also occur during vinegar elaboration by the traditional method. The vinegar biofilm produced by AAB on the air-liquid interface is primarily composed of cellulose and maintains the cells in close contact with oxygen. In this study, we screened for the ability of AAB to produce cellulose using different carbon sources in the presence or absence of ethanol...
February 2015: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/25221698/qualitative-analyses-of-the-antimicrobial-effect-of-ozonated-water-on-supragingival-plaque-and-salivary-microbes
#17
S Sadatullah, Nh Mohamed, Fa Razak
BACKGROUND: Ozone is an unstable gas, capable of oxidizing any biological entity. It is an effective bactericide in its gaseous as well as aqueous form. AIMS: The objective of this study was to determine the in-situ antimicrobial effect of 0.1 ppm ozonated water on plaque and salivary microorganisms. SUBJECTS AND METHODS: 24 h old supragingival plaque (SP) was collected from the two most posterior teeth in the contralateral quadrants before and after a 30 s rinse with either distilled water (control group) or 0...
July 2014: Annals of Medical and Health Sciences Research
https://www.readbyqxmd.com/read/25099661/fourier-transform-infra-red-spectroscopy-and-flow-cytometric-assessment-of-the-antibacterial-mechanism-of-action-of-aqueous-extract-of-garlic-allium-sativum-against-selected-probiotic-bifidobacterium-strains
#18
Jemma Booyens, Mapitsi Silvester Thantsha
BACKGROUND: It is generally reported that garlic (Allium sativum) harms pathogenic but not beneficial bacteria. Although numerous studies supporting the alleged garlic effects on pathogens are available, there are limited studies to prove this claim for beneficial bacteria. We have recently shown that garlic exhibits antibacterial activity against probiotic bifidobacteria. The aim of the current study was to elucidate the mechanism of action of garlic clove extract (GCE) on Bifidobacterium bifidum LMG 11041, B...
2014: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/25000844/occurrence-of-cryptosporidium-oocysts-and-giardia-cysts-in-effluent-from-sewage-treatment-plant-from-eastern-poland
#19
Jacek Sroka, Krzysztof Stojecki, Jolanta Zdybel, Jacek Karamon, Tomasz Cencek, Jacek Dutkiewicz
Cryptosporidium spp. and Giardia lamblia (synonyms: Giardia duodenalis, Giardia intestinalis) are emerging protozoa causing disease in humans and animals worldwide. These parasites can pose a serious threat to immunocompromised people, for whom the symptoms are more severe and may include abdominal pain, watery diarrhoea, nausea, headaches, malaise, and fever. One of the sources of these parasites can be treated wastewater from wastewater treatment plants (WTPs). Samples of treated wastewater (effluent), each of 10 L volume, were collected from 13 municipal WTPs located in eastern Poland...
2013: Annals of Agricultural and Environmental Medicine: AAEM
https://www.readbyqxmd.com/read/24565665/antimicrobial-activity-and-cytotoxicity-of-3-photosensitizers-activated-with-blue-light
#20
Florence Bulit, Iwona Grad, Daniel Manoil, Stéphane Simon, John C Wataha, Anna Filieri, Anis Feki, Jacques Schrenzel, Norbert Lange, Serge Bouillaguet
INTRODUCTION: Pulp repair is less likely to occur when dentin or pulpal tissue remains infected after caries excavation. Yet there are currently few options to kill residual bacteria without damaging resident cells. The current study has evaluated the effect of 3 blue light-activated chemicals on the viability of lactobacilli, odontoblast-like cells (MDPC-23), undifferentiated pulp cells (OD21), and human embryonic stem cells (hESC H1). METHODS: Bacteria were incubated for 15 minutes with curcumin, eosin Y, or rose bengal and then irradiated with blue light (240 seconds)...
March 2014: Journal of Endodontics
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