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https://www.readbyqxmd.com/read/28095835/antimicrobial-activity-of-some-plant-materials-used-in-armenian-traditional-medicine
#1
Mikayel Ginovyan, Margarit Petrosyan, Armen Trchounian
BACKGROUND: Antibiotic resistance has become one of the major problems facing humanity. The need for new antimicrobials has been increased dramatically. Plants are considered as one of the most promising sources for new antimicrobials discovery. Despite relatively small area, Armenia has large diversity of flora with many endemic species. In Armenian folk medicine plant materials have been used to treat various microbial diseases since ancient times. The goal of our research was to evaluate antimicrobial efficiency of different parts of five wild plants species which are commonly used in Armenian traditional medicine...
January 17, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28090273/the-effects-of-berberine-and-palmatine-on-efflux-pumps-inhibition-with-different-gene-patterns-in-pseudomonas-aeruginosa-isolated-from-burn-infections
#2
Seyed Sajjad Aghayan, Hamidreza Kalalian Mogadam, Mozhgan Fazli, Davood Darban-Sarokhalil, Seyed Sajjad Khoramrooz, Fereshteh Jabalameli, Somayeh Yaslianifard, Mehdi Mirzaii
BACKGROUND: Related Multidrug Resistance (MDR) to efflux pumps is a significant problem in treating infections caused by Pseudomonas aeruginosa (P. aeruginosa). Plant compounds have been identified as Pump Inhibitors (EPIs). In the current study, the potential effect of Berberine and Palmatine as EPIs were investigated on efflux pump inhibition through focusing on different gene patterns in P. aeruginosa isolated from burn infections. METHODS: All isolates were collected and identified using the standard biochemical tests...
January 2017: Avicenna Journal of Medical Biotechnology
https://www.readbyqxmd.com/read/28070663/the-pygidial-gland-secretion-of-the-forest-caterpillar-hunter-calosoma-calosoma-sycophanta-the-antimicrobial-properties-against-human-pathogens
#3
REVIEW
Marija Nenadić, Marina Soković, Jasmina Glamočlija, Ana Ćirić, Vesna Perić-Mataruga, Larisa Ilijin, Vele Tešević, Marina Todosijević, Ljubodrag Vujisić, Nikola Vesović, Srećko Ćurčić
Recently, various insect secretions have been tested as possible antimicrobial agents. In beetles, these secretions are essentially products of various exocrine glands, of which particular emphasis is on pygidial glands that are common for the suborder Adephaga. The antimicrobial potential of the pygidial gland secretion isolated from the adults of Calosoma sycophanta against human pathogens has been tested and compared with the potential of other carabid species, particularly within the tribe Carabini. The antimicrobial assay includes a microdilution method which was applied in order to determine the minimal inhibitory, minimal fungicidal and minimal bactericidal concentrations...
February 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28069649/antimicrobial-activity-of-ceftazidime-avibactam-when-tested-against-gram-negative-bacteria-isolated-from-patients-hospitalized-with-pneumonia-in-united-states-medical-centers-2011-2015
#4
Helio S Sader, Mariana Castanheira, Robert K Flamm
Bacterial isolates were collected from patients hospitalized with pneumonia (PHP), including ventilator-associated (VAP), from 76 USA medical centers in 2011-2015. The Gram-negative organisms (n=11,185; including 1,097 from VAP) were tested for susceptibility against ceftazidime-avibactam and comparators by broth microdilution method. β-lactamase-encoding genes were screened using a microarray based assay on selected isolates. Pseudomonas aeruginosa and Klebsiella spp. were the most common Gram-negatives isolated from PHP and VAP...
January 9, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28069328/antimicrobial-activity-of-ceftazidime-avibactam-and-comparator-agents-when-tested-against-bacterial-isolates-causing-infection-in-cancer-patients-2013-2014
#5
Helio S Sader, Mariana Castanheira, Ronald N Jones, Robert K Flamm
We evaluated the antimicrobial susceptibility of 623 Gram-negative organisms causing infection in patients with cancer in 52 United States hospitals (2013-2014) as part of the International Network for Optimal Resistance Monitoring (INFORM) program. Isolates were tested for susceptibility by broth microdilution method. β-lactamase encoding genes were evaluated for all Escherichia coli and Klebsiella spp. with an extended-spectrum β-lactamase (ESBL) phenotype by microarray-based assay. ESBL-phenotype was observed among 17...
November 29, 2016: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28068184/antimicrobial-susceptibilities-of-flavobacterium-psychrophilum-isolates-from-the-great-lakes-basin-michigan
#6
Danielle Van Vliet, Thomas P Loch, Peter Smith, Mohamed Faisal
Flavobacterium psychrophilum is a serious pathogen of salmonids worldwide, a matter that is compounded by the lack of effective vaccine preparations. As a result, biosecurity measures and antimicrobial agents remain the only available methods to control diseases caused by F. psychrophilum. It is feared that antimicrobial use may have led to the development of F. psychrophilum strains with reduced susceptibility. Therefore, the primary objective of this study was to determine the antimicrobial susceptibility profiles of 50 F...
January 9, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28062317/role-of-inoculum-and-mutant-frequency-on-fosfomycin-mic-discrepancies-by-agar-dilution-and-broth-microdilution-methods-in-enterobacteriaceae
#7
M Ballestero-Téllez, F Docobo-Pérez, J M Rodríguez-Martínez, M C Conejo, M S Ramos-Guelfo, J Blázquez, J Rodríguez-Baño, A Pascual
OBJECTIVES: Fosfomycin is re-evaluated as treatment of multidrug resistant Enterobacteriaceae infections. However, MIC differences have been described among the different susceptibility testing. The aim was to study the role of the different inoculum size used in agar dilution with respect broth microdilution, according to CLSI, in the fosfomycin MIC discrepancies. METHODS: Fosfomycin MICs were determined using agar dilution (reference) and broth microdilution in 220 Escherichia coli (81) and Klebsiella pneumoniae (139) clinical isolates...
January 3, 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/28062283/down-regulatory-effect-of-thymus-vulgaris-l-on-growth-and-tri4-gene-expression-in-fusarium-oxysporum-strains
#8
Kolsum Divband, Hojjatollah Shokri, Ali Reza Khosravi
The aims of this study were to evaluate the efficacy of Thymus vulgaris (T. vulgaris) essential oil on the fungal growth and Tri4 gene expression in Fusarium oxysporum (F. oxysporum) strains. The oil was obtained by water-distillation using a Clevenger-type system. The chemical composition of the essential oil was obtained by gas chromatography- mass spectroscopy (GC-MS) and by retention indices. The antifungal activity was evaluated by broth microdilution assay. A quantitative real time RT-PCR (qRT-PCR) assay was also developed specific for F...
January 3, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28056944/iridoids-from-canthium-subcordatum-iso-butanol-fraction-with-potent-biological-activities
#9
Christelle Joubouhi, Jean-de-Dieu Tamokou, David Ngnokam, Laurence Voutquenne-Nazabadioko, Jules-Roger Kuiate
BACKGROUND: The continuous emergence of multi-drug-resistant bacteria drastically reduces the efficacy of antibiotic armory and, consequently, increases the frequency of therapeutic failure. The discovery of new antibacterial drugs is an urgent need. The present study reports the antibacterial and antioxidant activities of the methanol extract, fractions and iridoids from Canthium subcordatum, a plant traditionally used as antidiabetic, anti-inflammatory, and antimicrobial. METHODS: Broth microdilution assay was used to determine minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) of extracts and iridoids against Staphylococcus aureus, Vibrio cholerae and Shigella flexneri...
January 5, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28053782/dexamethasone-abrogates-the-antimicrobial-and-antibiofilm-activities-of-different-drugs-against-clinical-isolates-of-staphylococcus-aureus-and-pseudomonas-aeruginosa
#10
Aquila Rodrigues, André Gomes, Pedro Henrique Ferreira Marçal, Marcus Vinícius Dias-Souza
Staphylococcus aureus and Pseudomonas aeruginosa are part of the human microbiota and are also important bacterial pathogens, for which therapeutic options are lacking nowadays. The combined administration of corticosteroids and antimicrobials is commonly used in the treatment of infectious diseases to control inflammatory processes and to minimize potential toxicity of antimicrobials, avoiding sequelae. Although different pharmaceutical dosage forms of antimicrobials combined to corticosteroids are available, studies on the interference of corticosteroids on the pharmacological activity of antimicrobials are scarce and controversial...
January 2017: Journal of Advanced Research
https://www.readbyqxmd.com/read/28053572/menadione-vitamin-k-enhances-the-antibiotic-activity-of-drugs-by-cell-membrane-permeabilization-mechanism
#11
Jacqueline C Andrade, Maria Flaviana B Morais Braga, Gláucia Morgana M Guedes, Saulo R Tintino, Maria A Freitas, Lucindo J Quintans, Irwin R A Menezes, Henrique D M Coutinho
Menadione, vitamin K3, belongs to the class of lipid-soluble vitamins and lipophilic substances as menadione cause disturbances in the bacterial membrane, resulting in damage to the fundamental elements for the integrity of the membrane, thus allowing increased permeability. Accordingly, the aim of this study was to evaluate in vitro the antibiotic-modifying activity of menadione in multiresistant strains of Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli, with a gradual increase in its subinhibitory concentration...
January 2017: Saudi Journal of Biological Sciences
https://www.readbyqxmd.com/read/28043557/antimycobacterial-potency-and-cytotoxicity-study-of-three-medicinal-plants
#12
Patrick Valere Tsouh Fokou, Regina Appiah-Opong, Dorothy Yeboah-Manu, Abena Adomah Kissi-Twum, Lauve Rachel Tchokouaha Yamthe, Aristide Laurel Mokale Kognou, Phyllis Addo, Fabrice Fekam Boyom, Alexander Kwadwo Nyarko
OBJECTIVE/BACKGROUND: Mycobacterial infections including tuberculosis, leprosy, and buruli ulcer are among the most prevalent, debilitating, and deadly tropical diseases, especially in Sub-Saharan Africa. The development of drug resistance to the currently available drugs and the poor compliance emphasize the need for new chemotherapeutic agents. This study was designed to evaluate the in vitro activity of Cleistopholis patens, Annona reticulata, and Greenwayodendron suaveolens against Mycobacterium smegmatis...
December 2016: International Journal of Mycobacteriology
https://www.readbyqxmd.com/read/28012417/synthesis-and-characterization-of-mns2-reduced-graphene-oxide-nanohybrids-for-with-photocatalytic-and-antibacterial-activity
#13
Ali Fakhri, Delaram Salehpour Kahi
A facile one-step hydrothermal route was developed here to prepare MnS2/reduced graphene oxide nanohybrids. The crystal morphologies could be controlled by adjusting the solvent, surfactant, and pH of the precursor solution. X-ray diffraction (XRD), field-emission scanning electron microscopy (FE-SEM), transmission electron microscopy (TEM), UV-Vis absorption spectra, and photoluminescence spectra (PL), were used to characterize the structures of the samples were used to characterize the structures of the samples, and the specific surface area was determined using the Brunauer-Emmett-Teller (BET) method...
January 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28006749/%C3%AE-ag2moo4-microcrystals-characterization-antibacterial-properties-and-modulation-analysis-of-antibiotic-activity
#14
J V B Moura, T S Freitas, R P Cruz, R L S Pereira, A R P Silva, A T L Santos, J H da Silva, C Luz-Lima, P T C Freire, H D M Coutinho
This study reports the antibacterial properties and modulation analysis of antibiotic activity by β-Ag2MoO4 microcrystals as well as their structural and vibrational characterization. The silver molybdate was obtained by the conventional hydrothermal method, and the structural, vibrational and morphological properties of the sample were determined using X-ray diffraction, Raman spectroscopy and scanning electron microscopy images. β-Ag2MoO4 microcrystals obtained show spinel-type cubic structure (Fd-3m) with irregular shapes...
December 19, 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/27981797/laserpitium-zernyi-hayek-flower-and-herb-extracts-phenolic-compounds-and-anti-edematous-antioxidant-and-antimicrobial-activities
#15
Višnja B Popović, Maja A Tomić, Radica M Stepanović-Petrović, Ana M Micov, Marina T Milenković, Silvana D Petrović, Ljuboš J Ušjak, Marjan S Niketić
Phenolic compounds and different biological activities of the dry methanol extracts of the flowers and the herb (aerial parts without flowers) of Laserpitium zernyi Hayek (Apiaceae) were investigated. The total phenolic contents in the extracts were determined spectrophotometrically using Folin-Ciocalteu reagent. In both extracts, apigenin, luteolin, their 7-O-glucosides and chlorogenic acid were detected by HPLC. Identified phenolics were quantified in both extracts, except luteolin in L. zernyi herb extract...
December 16, 2016: Chemistry & Biodiversity
https://www.readbyqxmd.com/read/27942360/antibacterial-activity-of-silver-nanoparticles-and-their-combination-with-zataria-multiflora-essential-oil-and-methanol-extract
#16
Shirin Sheikholeslami, Seyyedeh Elaheh Mousavi, Hamid Reza Ahmadi Ashtiani, Seyed Reza Hosseini Doust, Seyed Mahdi Rezayat
BACKGROUND: Against a variety of antimicrobial resistant pathogens, the scientists attempted substitution of antimicrobial medicine with various nanoparticles and plant-based antibacterial substances. OBJECTIVES: The aim of this study was to assess the antibacterial effects of silver nanoparticles solely and in combination with Zataria multiflora essential oil and methanolic extract on some photogenic bacteria. METHODS: Minimum inhibitory concentrations (MICs) and fractional inhibitory concentrations (FICs) of plant essential oil, methanolic extract, and silver nanoparticles against bacteria were evaluated using the broth microdilution method and check board microtiter assays...
October 2016: Jundishapur Journal of Microbiology
https://www.readbyqxmd.com/read/27937097/treatment-of-helicobacter-pylori-infected-mice-with-bryophyllum-pinnatum-a-medicinal-plant-with-antioxidant-and-antimicrobial-properties-reduces-bacterial-load
#17
Laure Brigitte Kouitcheu Mabeku, Bertrand Eyoum Bille, Thibau Flaurant Tchouangueu, Eveline Nguepi, Hubert Leundji
CONTEXT: Bryophyllum pinnatum (Lam.) Kurz (Crassulaceae) is a plant known for its antiulcer properties. OBJECTIVE: This study evaluates the anti-Helicobacter pylori activity of Bryophyllum pinnutum methanol extract with a mouse model and its antioxidant properties. MATERIALS AND METHODS: Dried leaves of Bryophyllum pinnutum were extracted with methanol and ethyl acetate. Broth microdilution method was used to evaluate the anti-Helicobacter activity of extract samples in vitro...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/27936245/in-vitro-selective-growth-inhibitory-effect-of-8-hydroxyquinoline-on-clostridium-perfringens-versus-bifidobacteria-in-a-medium-containing-chicken-ileal-digesta
#18
Eva Skrivanova, Filip Van Immerseel, Petra Hovorkova, Ladislav Kokoska
Clostridium perfringens-induced necrotic enteritis is generally controlled by antibiotics. However, because of increasing antibiotic resistance, other antibacterial agents are required, preferably ones that do not affect the beneficial intestinal microbiota of the host. This study evaluated the in vitro selective growth-inhibitory effect of 8-hydroxyquinoline (8HQ) on C. perfringens vs. bifidobacteria in a medium containing chicken ileal digesta. Prior to the experiments, the minimum inhibitory concentrations of 8HQ and penicillin G were determined by broth microdilution assay...
2016: PloS One
https://www.readbyqxmd.com/read/27935770/from-breast-cancer-to-antimicrobial-combating-extremely-resistant-gram-negative-superbugs-using-novel-combinations-of-polymyxin-b-with-selective-estrogen-receptor-modulators
#19
Maytham H Hussein, Elena K Schneider, Alysha G Elliott, Meiling Han, Felisa Reyes-Ortega, Faye Morris, Mark A T Blastovich, Raad Jasim, Bart Currie, Mark Mayo, Mark Baker, Matthew A Cooper, Jian Li, Tony Velkov
Novel therapeutic approaches are urgently needed to combat nosocomial infections caused by extremely drug-resistant (XDR) "superbugs." This study aimed to investigate the synergistic antibacterial activity of polymyxin B in combination with selective estrogen receptor modulators (SERMs) against problematic Gram-negative pathogens. In vitro synergistic antibacterial activity of polymyxin B and the SERMs tamoxifen, raloxifene, and toremifene was assessed using the microdilution checkerboard and static time-kill assays against a panel of Gram-negative isolates...
December 9, 2016: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/27931688/antimycobacterial-mechanism-of-vanillin-involves-disruption-of-cell-surface-integrity-virulence-attributes-and-iron-homeostasis
#20
Sharda Sharma, Rahul Pal, Saif Hameed, Zeeshan Fatima
OBJECTIVE/BACKGROUND: Tuberculosis (TB) remains a global threat, claiming one-third of the population annually. The ever increasing emergence of multidrug-resistant TB (MDR-TB) is the major impediment to effective anti-TB therapy. Under such circumstances, deciphering the antimycobacterial potential of natural compounds has gained considerable prominence. This study evaluated the antimycobacterial activity of vanillin (Van), a natural food-flavoring agent and preservative, along with its potential mechanisms of action...
December 2016: International Journal of Mycobacteriology
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