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Microdilution assay

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https://www.readbyqxmd.com/read/29234423/antifungal-activity-of-essential-oils-against-candida-albicans-strains-isolated-from-users-of-dental-prostheses
#1
Julliana Cariry Palhano Freire, José Klidenberg de Oliveira Júnior, Daniele de Figueredo Silva, Janiere Pereira de Sousa, Felipe Queiroga Sarmento Guerra, Edeltrudes de Oliveira Lima
Objective: The objective of this study was to analyze the antifungal activity of citral, selected by screening natural products, against Candida albicans isolates from subjects who use dental prostheses. Methodology: Screening of essential oils, including those from Mentha piperita L. (Briq), Origanum vulgare, and Zingiber officinale L., and the phytoconstituents citral and limonene, to select an appropriate natural product. Citral, which mediated the best antifungal response, was selected for biological assays...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/29234399/antimicrobial-activities-and-time-kill-kinetics-of-extracts-of-selected-ghanaian-mushrooms
#2
Theresa Appiah, Yaw Duah Boakye, Christian Agyare
The rapid rise of antimicrobial resistance is a worldwide problem. This has necessitated the need to search for new antimicrobial agents. Mushrooms are rich sources of potential antimicrobial agents. This study investigated the antimicrobial properties of methanol extracts of Trametes gibbosa, Trametes elegans, Schizophyllum commune, and Volvariella volvacea. Agar well diffusion, broth microdilution, and time-kill kinetic assays were used to determine the antimicrobial activity of the extracts against selected test organisms...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/29229883/in-vitro-leishmanicidal-antibacterial-and-antitumour-potential-of-anhydrocochlioquinone-a-obtained-from-the-fungus-cochliobolus-sp
#3
Fernanda F Campos, Jonas P Ramos, Djalma M DE Oliveira, Tania M A Alves, Elaine M DE Souza-Fagundes, Carlos L Zani, Fabio C Sampaio, Attilio Converti, Betania B Cota
The bioassay-guided fractionation of the ethyl acetate extract of the fungus Cochliobolus sp. highlighted leishmanicidal activity and allowed for anhydrocochlioquinone A (ANDC-A) isolation. MS, 1D and 2D NMR spectra of this compound were in agreement with those published in the literature. ANDC-A exhibited leishmanicidal activity with EC 50 value of 22.4 microgram/mL (44 mu M) and, when submitted to the microdilution assay against Gram-ositive and Gram-negative bacteria, showed a minimal inhibitory concentration against Staphylococcus aureus ATCC 25295 of 128 microgram/mL (248...
December 2017: Journal of Biosciences
https://www.readbyqxmd.com/read/29228818/evaluation-of-herbal-concoctions-sold-at-ga-maja-limpopo-province-in-south-africa-and-in-vitro-pharmacological-evaluation-of-plants-used-to-manufacture-the-concoctions
#4
Mash M Matotoka, Peter Masoko
The aim of the study was to evaluate the biological activities and safety of commercial herbal concoctions manufactured in Ga Maja (Limpopo province). Microbial contamination was evaluated by spread-plating the concoctions on agar plates. The VITEK 2 instrument was used for identification of the pure cultures. Nutritional content of the concoctions was determined. Thin layer chromatography was used to analyze the chemical constituents of the extracts. The microdilution assay and bioautography were used to evaluate antimicrobial activity against selected microorganisms...
January 1, 2017: Journal of Evidence-based Complementary & Alternative Medicine
https://www.readbyqxmd.com/read/29225645/the-effects-of-different-ionic-liquid-coatings-and-the-length-of-alkyl-chain-on-antimicrobial-and-cytotoxic-properties-of-silver-nanoparticles
#5
Abbas Abbaszadegan, Ahmad Gholami, Sara Abbaszadegan, Zeynab Sadat Aleyasin, Yasamin Ghahramani, Samira Dorostkar, Bahram Hemmateenejad, Younes Ghasemi, Hashem Sharghi
Introduction: The antibacterial efficacy and toxicity of silver nanoparticles (AgNPs) depends on their physicochemical properties including size, shape, surface charge and surface coatings. The Objectives of this study were: i) To synthesize and characterize positively charged AgNPs coated by different ionic-liquids with different alkyl chain lengths, ii) To evaluate the antimicrobial activity of these nanoparticles against Enterococcus faecalis compared to sodium hypochlorite (NaOCl) and chlorhexidine (CHX), iii) To compare the cytocompatibility of these solutions against L929 mouse fibroblasts...
2017: Iranian Endodontic Journal
https://www.readbyqxmd.com/read/29219046/molecular-cloning-and-functional-characterization-of-antimicrobial-peptides-brevinin-1ulf-and-ulmin-1ula-in-the-skin-of-the-newly-classified-ryukyu-brown-frog-rana-ulma
#6
Daisuke Ogawa, Maki Mochitate, Maho Furukawa, Itaru Hasunuma, Tetsuya Kobayashi, Sakae Kikuyama, Shawichi Iwamuro
Antimicrobial peptides (AMPs) were previously isolated from the skin of the Ryukyu brown frog Rana okinavana. However, this species has recently been reclassified as two species, i.e., Rana kobai and Rana ulma. As a result, it was determined that AMPs isolated from R. okinavana were in fact products of R. kobai, but not of R. ulma. In the present study, we collected skin samples from the species R. ulma and cloned twelve cDNAs encoding AMP precursors for the acyclic brevinin-1ULa--1ULf, the temporin-ULa-ULc, ranatuerin-2ULa, japonicin-1ULa, and a novel peptide using reverse-transcription polymerase chain reaction techniques...
December 2017: Zoological Science
https://www.readbyqxmd.com/read/29216350/italian-nationwide-survey-on-pseudomonas-aeruginosa-from-invasive-infections-activity-of-ceftolozane-tazobactam-and-comparators-and-molecular-epidemiology-of-carbapenemase-producers
#7
Tommaso Giani, Fabio Arena, Simona Pollini, Vincenzo Di Pilato, Marco Maria D'Andrea, Lucia Henrici De Angelis, Matteo Bassetti, Gian Maria Rossolini
Objectives: Pseudomonas aeruginosa is a major cause of severe healthcare-associated infections and often shows MDR phenotypes. Ceftolozane/tazobactam is a new cephalosporin/β-lactamase inhibitor combination with potent activity against P. aeruginosa. This survey was carried out to evaluate the susceptibility of P. aeruginosa, circulating in Italy, to ceftolozane/tazobactam and comparators and to investigate the molecular epidemiology of carbapenemase-producing strains. Methods: Consecutive non-replicate P...
December 5, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29214974/insights-into-the-candidacidal-mechanism-of-ctn-15-34-a-carboxyl-terminal-crotalicidin-derived-peptide-related-to-cathelicidins
#8
Carolina Sidrim P Cavalcante, Francisca Lidiane Linhares de Aguiar, Raquel O S Fontenelle, Ramon Roseo de P P B de Menezes, Alice Maria Costa Martins, Cláudio B Falcão, David Andreu, Gandhi Rádis-Baptista
PURPOSE: Ctn[15-34], a carboxyl-terminal fragment of crotalicidin (a cathelicidin from the venom gland of a South American rattlesnake), has shown antifungal activity against clinical and standard strains of Candida species. The aim of the present work was to investigate the underlying mechanisms of the candidicidal activity of Ctn[15-34]. METHODOLOGY: The time-kill profile and drug synergism were evaluated by means of a microdilution assay and multi-parametric flow cytometry...
December 7, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/29208359/aminoguanidine-hydrazones-agh-s-as-modulators-of-norfloxacin-resistance-in-staphylococcus-aureus-that-overexpress-nora-efflux-pump
#9
Natalina Dantas, Thiago Mendonça de Aquino, João Xavier de Araújo-Júnior, Edeildo da Silva-Júnior, Ednaldo Almeida Gomes, Antoniel Augustos Severo Gomes, José Pinto Siqueira-Júnior, Francisco Jaime Bezerra Mendonça Junior
One of the promising fields for improving the effectiveness of antimicrobial agents is their combination with efflux pump inhibitors (EPIs), which besides expanding the use of existing antibiotics. The goal of this research was to evaluate a series of aminoguanidine hydrazones (AGH's, 1-19) as antibacterial agents and NorA efflux pump inhibitors in Staphylococcus aureus strain SA-1199B. Molecular modeling and docking studies were also performed in order to explain at the molecular level the interactions of the compounds with the generated NorA efflux pump model...
December 2, 2017: Chemico-biological Interactions
https://www.readbyqxmd.com/read/29206750/ceftazidime-avibactam-antimicrobial-activity-and-spectrum-when-tested-against-gram-negative-organisms-from-pediatric-patients-results-from-the-inform-surveillance-program-united-states-2011-2015
#10
Helio S Sader, Michael D Huband, Leonard R Duncan, Robert K Flamm
BACKGROUND: Ceftazidime-avibactam was approved by the US Food and Drug Administration in 2015 to treat complicated intra-abdominal and urinary tract infections in adults, and is under clinical development for treating pediatric patients. METHODS: Among 53,381 gram-negative organisms (1 per patient) collected in 2011-2015, 8,461 (15.9%) were from pediatric (≤17 years-old [yo]) patients. The isolates were collected from 82 US medical centers and susceptibility tested against ceftazidime-avibactam (avibactam at fixed 4 µg/mL) and comparators by reference broth microdilution methods...
December 4, 2017: Pediatric Infectious Disease Journal
https://www.readbyqxmd.com/read/29206142/composition-and-antibacterial-activity-of-the-essential-oils-of-orthosiphon-stamineus-benth-and-ficus-deltoidea-jack-against-pathogenic-oral-bacteria
#11
Nuramirah Azizan, Shahida Mohd Said, Zamirah Zainal Abidin, Ibrahim Jantan
In this study, the essential oils of Orthosiphon stamineus Benth and Ficus deltoidea Jack were evaluated for their antibacterial activity against invasive oral pathogens, namely Enterococcus faecalis, Streptococcus mutans, Streptococcus mitis, Streptococcus salivarius, Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis and Fusobacterium nucleatum. Chemical composition of the oils was analyzed using gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). The antibacterial activity of the oils and their major constituents were investigated using the broth microdilution method (minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC))...
December 5, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29190763/in-vitro-activity-of-spirulina-platensis-water-extract-against-different-candida-species-isolated-from-vulvo-vaginal-candidiasis-cases
#12
Antonella Marangoni, Claudio Foschi, Matteo Micucci, Rogers Alberto Nahui Palomino, Tullia Gallina Toschi, Beatrice Vitali, Luca Camarda, Mara Mandrioli, Marta De Giorgio, Rita Aldini, Ivan Corazza, Alberto Chiarini, Roberto Cevenini, Roberta Budriesi
The high incidence of vulvo-vaginal candidiasis, combined with the growing problems about azole resistance and toxicity of antifungal drugs, highlights the need for the development of new effective strategies for the treatment of this condition. In this context, natural compounds represent promising alternatives. The cyanobacterium Spirulina platensis, a blue-green alga, exhibits antimicrobial activities against several microorganisms. Nevertheless, only few data about the antifungal properties of Spirulina platensis are available and its potential toxic effects have not been largely investigated...
2017: PloS One
https://www.readbyqxmd.com/read/29189730/a-new-series-of-pyrrole-based-chalcones-synthesis-and-evaluation-of-antimicrobial-activity-cytotoxicity-and-genotoxicity
#13
Ahmet Özdemir, Mehlika Dilek Altıntop, Belgin Sever, Hülya Karaca Gençer, Handan Açelya Kapkaç, Özlem Atlı, Merve Baysal
In an effort to develop new potent antimicrobial and anticancer agents, new pyrrole-based chalcones were designed and synthesized via the base-catalyzed Claisen-Schmidt condensation of 2-acetyl-1-methylpyrrole with 5-(aryl)furfural derivatives. The compounds were evaluated for their in vitro antimicrobial effects on pathogenic bacteria and Candida species using microdilution and ATP luminescence microbial cell viability assays. MTT assay was performed to determine the cytotoxic effects of the compounds on A549 human lung adenocarcinoma, HepG2 human hepatocellular carcinoma, C6 rat glioma, and NIH/3T3 mouse embryonic fibroblast cell lines...
November 30, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29186432/bacterial-zincophore-s-s-ethylenediamine-n-n-disuccinic-acid-is-an-effective-inhibitor-of-mbls
#14
Anna Proschak, Jan Kramer, Ewgenij Proschak, Thomas A Wichelhaus
Objectives: Carbapenemases such as MBLs are spreading among Gram-negative bacterial pathogens. Infections due to these MDR bacteria constitute a major global health challenge. Therapeutic strategies against carbapenemase-producing bacteria include β-lactamase inhibitor combinations. [S,S]-ethylenediamine-N,N'-disuccinic acid (EDDS) is a chelator and potential inhibitor of MBLs. We investigated the activity of EDDS in combination with imipenem against MBL-producing bacteria in vitro as well as in vivo...
November 23, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29177837/high-throughput-flow-cytometry-screening-of-multidrug-efflux-systems
#15
Mark K Haynes, Matthew Garcia, Ryan Peters, Anna Waller, Pietro Tedesco, Oleg Ursu, Cristian G Bologa, Radleigh G Santos, Clemencia Pinilla, Terry H Wu, Julie A Lovchik, Tudor I Oprea, Larry A Sklar, George P Tegos
The resistance nodulation cell division (RND) family of proteins are inner membrane transporters that associate with periplasmic adaptor proteins and outer membrane porins to affect substrate transport from the cytosol and periplasm in Gram-negative bacteria. Various structurally diverse compounds are substrates of RND transporters. Along with their notable role in antibiotic resistance, these transporters are essential for niche colonization, quorum sensing, and virulence as well as for the removal of fatty acids and bile salts...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29156575/synthesis-of-oxadiazole-thiadiazole-hybrids-and-their-anticandidal-activity
#16
Serkan Levent, Betül Kaya Çavuşoğlu, Begüm Nurpelin Sağlık, Derya Osmaniye, Ulviye Acar Çevik, Özlem Atlı, Yusuf Özkay, Zafer Asım Kaplancıklı
In the field of infection management, it is a major challenge to discover a potent and safe antifungal agent due to the emergence of resistant strains. Hence, the goal of this paper is to design and synthesize novel oxadiazole-thiadiazole hybrid compounds (6a-6s) and evaluate their antifungal activity. The structures of synthesized compounds were elucidated by various methods including FT-IR, ¹H-NMR, (13)C-NMR and HR-MS spectral data. Compounds were tested against four Candida species by broth microdilution assay...
November 18, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29155212/effect-of-isoquercitrin-on-membrane-dynamics-and-apoptosis-like-death-in-escherichia-coli
#17
JiEun Yun, Eun-Rhan Woo, Dong Gun Lee
Minimum inhibitory concentration (MIC) is defined as the lowest concentration of a compound that completely inhibits microbial growth. Antibacterial mechanisms of compounds have been investigated at their sub-MICs as well as at their MIC. In this study, the effects of sub-MIC and MIC of isoquercitrin on Escherichia coli were investigated. The antibacterial effect of isoquercitrin was tested using the microdilution method. Sub-MICs of isoquercitrin induced the production of reactive oxygen species and depletion of glutathione...
November 16, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29145848/pharmacological-potential-of-bidens-pilosa-l-and-determination-of-bioactive-compounds-using-uhplc-qqqlit-ms-ms-and-gc-ms
#18
Garima Singh, Ajit Kumar Passsari, Pratibha Singh, Vincent Vineeth Leo, Sarathbabu Subbarayan, Brijesh Kumar, Bhim Pratap Singh, Hauzel Lalhlenmawia, Nachimuthu Senthil Kumar
BACKGROUND: Research of natural products from traditionally used medicinal plants to fight against the human ailments is fetching attention of researchers worldwide. Bidens pilosa Linn. var. Radiata (Asteraceae) is well known for its folkloric medicinal use against various diseases from many decades. Mizoram, North East India, has high plant diversity and the use of this plant as herbal medicine is deep rooted in the local tribes. The present study was executed to understand the pharmacological potential of B...
November 16, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/29133568/activity-of-ceftolozane-tazobactam-against-carbapenem-resistant-carbapenemase-non-producing-pseudomonas-aeruginosa-and-associated-resistance-mechanisms
#19
Yu Mi Wi, Kerryl E Greenwood-Quaintance, Audrey N Schuetz, Kwan Soo Ko, Kyong Ran Peck, Jae-Hoon Song, Robin Patel
Although carbapenems are effective for treating serious multidrug-resistant Pseudomonas aeruginosa infections, carbapenem-resistant P. aeruginosa (CRPA) is now being reported worldwide. Ceftolozane/tazobactam (C/T) demonstrates activity against many multidrug-resistant isolates. We evaluated the activity of C/T and compared its activity to that of ceftazidime/avibactam (C/A) using a well-characterized collection of carbapenemase non-producing CRPA isolates. Forty-two carbapenemase non-producing CRPA isolates from a previous study (Int J Antimicrob Agents 2012;40:168-72) were included...
November 13, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29116300/cymbopogon-citratus-essential-oil-effect-on-polymicrobial-caries-related-biofilm-with-low-cytotoxicity
#20
Maria Alcionéia Carvalho de Oliveira, Aline Chiodi Borges, Fernanda Lourenção Brighenti, Marcos José Salvador, Aline Vidal Lacerda Gontijo, Cristiane Yumi Koga-Ito
The objective of this study was to evaluate the effects of Cymbopogon citratus essential oil and its main compound (citral) against primary dental colonizers and caries-related species. Chemical characterization of the essential oil was performed by gas chromatography/mass spectroscopy (GC/MS), and the main compound was determined. Antimicrobial activity was tested against Actinomyces naeslundii, Lactobacillus acidophilus, S. gordonii, S. mitis, S. mutans, S. sanguinis and S. sobrinus. Minimum inhibitory and bactericide concentrations were determined by broth microdilution assay for streptococci and lactobacilli reference, and for clinical strains...
November 6, 2017: Brazilian Oral Research
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