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https://www.readbyqxmd.com/read/29650464/clioquinol-an-alternative-antimicrobial-agent-against-common-pathogenic-microbe
#1
Z You, X Ran, Y Dai, Y Ran
Skin and soft tissue infections (SSTIs) are very common in dermatology and the use of antimicrobial formulations are important in treating these diseases. With the increasing of drug-resistant strains, researchers need to find ways to enhance the effectiveness and/or reduce the drug resistance. Clioquinol was one of antiseptics that can inactivate microbes. It was lack of data of antimicrobial activity; meanwhile it was infrequently used in infection. In order to research the antimicrobial spectrum and activity of topical 3% clioquinol cream among common pathogenic microorganisms compared with other common topical pharmaceuticals, we used modified agar diffusion assay to judge drug susceptibility and compared with broth microdilution assay...
April 10, 2018: Journal de Mycologie Médicale
https://www.readbyqxmd.com/read/29644192/is-antifungal-resistance-a-cause-for-treatment-failure-in-dermatophytosis-a-study-focused-on-tinea-corporis-and-cruris-from-a-tertiary-centre
#2
Kabir Sardana, Ravinder Kaur, Pooja Arora, Ritu Goyal, Sneha Ghunawat
Background: Dermatophytoses are one of the most common skin diseases that have been largely simple to treat. However, in recent years, these infections have become recalcitrant to treatment which can possibly be due to antifungal resistance. Aim: To analyze the resistance pattern of patients with recalcitrant dermatophytoses. Materials and Methods: A cross-sectional evaluation was undertaken of 40 consecutive patients with recalcitrant tinea corporis/cruris/both who had taken systemic antifungal treatment and did not respond completely to therapy or had recurrent lesion within 1 month of stopping the therapy...
March 2018: Indian Dermatology Online Journal
https://www.readbyqxmd.com/read/29642832/-determination-of-the-role-of-salmonella-enterica-plasmids-in-antibiotic-susceptibility-and-caenorhabditis-elegans-pathogenicity
#3
Deniz Aksoy, Ece Şen
Poultry animals and poultry associated products are important risk sources for Salmonellosis. S.Kentucky and S.Infantis are among the serovars frequently isolated from retail chickens and were reported to be isolated in Turkey. In this study, the role of plasmids carried by S.Kentucky and S.Infantis isolates in antibiotic resistance profiles of the isolates and their pathogenicity on Caenorhabditis elegans nematode model system were investigated. The isolates used, 1 of Kentucky and 2 of Infantis serotypes, were selected among food-borne Salmonella isolated from chicken carcass in Edirne...
January 2018: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/29611435/chemical-composition-and-in-vitro-antibacterial-and-antiproliferative-activities-of-the-essential-oil-from-the-leaves-of-psidium-myrtoides-o-berg-myrtaceae
#4
Alline L B Dias, Hellen R F Batista, Elisângela B B Estevam, Cassia C F Alves, Moacir R Forim, Heloiza D Nicolella, Ricardo A Furtado, Denise C Tavares, Thayna S Silva, Carlos H G Martins, Mayker L D Miranda
In this study, the chemical composition and antibacterial and antiproliferative potential of the essential oil obtained from fresh leaves of Psidium myrtoides (PM-EO) against oral pathogens and human tumour cell lines were investigated for the first time. GC-FID and GC-MS analyses showed that trans-β-caryophyllene (30.9%), α-humulene (15.9%), α-copaene (7.8%), caryophyllene oxide (7.3%) and α-bisabolol (5.3%) are the major constituents of PM-EO. The antibacterial activity of PM-EO against a panel of oral pathogens was investigated in terms of their minimal inhibitory concentrations (MIC) using the broth microdilution method...
April 3, 2018: Natural Product Research
https://www.readbyqxmd.com/read/29600051/-in-vitro-effects-of-n-acetylcysteine-alone-and-combined-with-tigecycline-on-planktonic-cells-and-biofilms-of-acinetobacter-baumannii
#5
Jinlun Feng, Baomo Liu, Junwen Xu, Qinqin Wang, Lixia Huang, Weijun Ou, Jincui Gu, Jian Wu, Shaoli Li, Chao Zhuo, Yanbin Zhou
Background: Acinetobacter baumannii ( A. baumannii ), as a common opportunistic pathogen, has strong ability to form biofilms, which has led to drug resistance and chronic infections. The combination of N-acetylcysteine (NAC) and tigecycline (TGC) was demonstrated to synergistically inhibit biofilm-associated bacterial infections, including methicillin-resistant Staphylococcus aureus and Staphylococcus epidermidis. The purpose of this study is to investigate the effect of NAC and TGC on planktonic cells and biofilms of A...
January 2018: Journal of Thoracic Disease
https://www.readbyqxmd.com/read/29593912/antimicrobial-susceptibility-among-colistin-sulbactam-and-fosfomycin-and-a-synergism-study-of-colistin-in-combination-with-sulbactam-or-fosfomycin-against-clinical-isolates-of-carbapenem-resistant-acinetobacter-baumannii
#6
Sombat Leelasupasri, Wichai Santimaleeworagun, Tossawan Jitwasinkul
This in vitro study aimed to determine the activity of colistin plus sulbactam and colistin plus fosfomycin against carbapenem-resistant A. baumannii (CRAB). Fifteen clinical isolates were obtained from patients admitted to Phyathai II International Hospital, Bangkok, Thailand, from August 2014 to April 2015. The antimicrobial susceptibilities of colistin, sulbactam, and fosfomycin were evaluated using the E-test or broth microdilution and the synergistic activity of the antibacterial combinations (colistin plus sulbactam or fosfomycin) was determined using the chequerboard method...
2018: Journal of Pathogens
https://www.readbyqxmd.com/read/29590370/kpc-2-carbapenemase-producing-pseudomonas-aeruginosa-reaching-germany
#7
Jürgen Benjamin Hagemann, Niels Pfennigwerth, Sören G Gatermann, Heike von Baum, Andreas Essig
Background: Antimicrobial resistance due to carbapenemase expression poses a worldwide threat in healthcare. Inter-genus exchange of genetic information is of utmost importance in this context. Objectives: Here, to the best of our knowledge, we describe the first detection and characterization of a KPC-2-producing Pseudomonas aeruginosa in Germany. Methods: Characterization of the isolate was performed using MALDI-TOF MS, automated microdilution and MLST...
March 24, 2018: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29576705/antibacterial-activity-of-azadirachta-indica-pongamia-pinnata-psidium-guajava-and-mangifera-indica-and-their-mechanism-of-action-against-streptococcus-mutans
#8
Dikonketso Cathrine Bodiba, Preety Prasad, Ajay Srivastava, Brigdet Crampton, Namrita Sharan Lall
Background: Curative plants have reportedly been used to make chewing sticks/toothbrushes intended for the treatment of oral diseases. Objective: The in vitro antibacterial activities of Azadirachta indica , Pongamia pinnata , Psidium guajava , and Mangifera indica were evaluated against Streptococcus mutans , along with the cytotoxicity and antioxidant and synergistic potentials. The effect of M. indica on the expression of crucial virulence genes spaP and gtfB of S...
January 2018: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/29575121/manuka-honey-and-methylglyoxal-increase-the-sensitivity-of-staphylococcus-aureus-to-linezolid
#9
Gabrielle Hayes, Nicole Wright, Sarah L Gardner, Calla L Telzrow, Andrew J Wommack, Patrick A Vigueira
The continued emergence and global spread of bacterial antibiotic resistance has fueled the search for novel antimicrobial agents and resistance-modifying compounds. Manuka honey has both antimicrobial properties and the ability to increase the efficacy of FDA-approved antibiotic drugs. Compared to other types of honey, manuka honey contains elevated levels of methylglyoxal, a small molecule that contributes to its antibacterial activity. Manuka honey has shown particular promise for the treatment of antibiotic-resistant Gram-positive organisms such as methicillin-resistant Staphylococcus aureus...
March 25, 2018: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/29568291/macroalgae-extracts-from-antarctica-have-antimicrobial-and-anticancer-potential
#10
Rosiane M Martins, Fernanda Nedel, Victoria B S Guimarães, Adriana F da Silva, Pio Colepicolo, Claudio M P de Pereira, Rafael G Lund
Background: Macroalgae are sources of bioactive compounds due to the large number of secondary metabolites they synthesize. The Antarctica region is characterized by extreme weather conditions and abundant aggregations of macroalgae. However, current knowledge on their biodiversity and their potential for bio-prospecting is still fledging. This study evaluates the antimicrobial and cytotoxic activity of different extracts of four macroalgae ( Cystosphaera jacquinotii, Iridaea cordata, Himantothallus grandifolius , and Pyropia endiviifolia ) from the Antarctic region against cancer and non-cancer cell lines...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29553507/broth-microdilution-in-vitro-screening-an-easy-and-fast-method-to-detect-new-antifungal-compounds
#11
Calliandra Maria de-Souza-Silva, Fernanda Guilhelmelli, Daniel Zamith-Miranda, Marco Antônio de Oliveira, Joshua Daniel Nosanchuk, Ildinete Silva-Pereira, Patrícia Albuquerque
Fungal infections have become an important medical condition in the last decades, but the number of available antifungal drugs is limited. In this scenario, the search for new antifungal drugs is necessary. The protocol reported here details a method to screen peptides for their antifungal properties. It is based on the broth microdilution susceptibility test from the Clinical and Laboratory Standards Institute (CLSI) M27-A3 guidelines with modifications to suit the research of antimicrobial peptides as potential new antifungals...
February 14, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29534201/in-vitro-activity-of-amphotericin-b-azole-combinations-against-malassezia-pachydermatis-strains
#12
Sergio Álvarez-Pérez, Marta E García, José L Blanco
Combination therapy has become popular in clinical practice, but limited data on the effects of combinations of antifungal agents is still available for most fungal pathogens. We studied the in vitro response of 30 genetically diverse clinical strains of the basidiomycetous lipophilic yeast Malassezia pachydermatis obtained from cases of canine otitis to several amphotericin B (AMB)-azole combinations. Broth microdilution checkerboard tests revealed that AMB antagonized the effects of itraconazole (ITC) and voriconazole (VRC) in 50% and 6...
March 8, 2018: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/29530608/frankincense-and-myrrh-essential-oils-and-burn-incense-fume-against-micro-inhabitants-of-sacral-ambients-wisdom-of-the-ancients
#13
Milica Ljaljević Grbić, Nikola Unković, Ivica Dimkić, Peđa Janaćković, Milan Gavrilović, Olja Stanojević, Miloš Stupar, Ljubodrag Vujisić, Aleksa Jelikić, Slaviša Stanković, Jelena Vukojević
ETHNOPHARMACOLOGICAL RELEVANCE: Essential oils obtained from resins of Boswellia carteri Birdw. and Commiphora myrrha (Nees) Engl., commonly known as frankincense and true myrrh respectively, have been used extensively since 2800 BCE for the treatment of skin sores, wounds, teeth, inflammation, and urinary tract diseases in traditional medicine; for preparation of mummification balms and unguents; and also as incense and perfumes. Since ancient times, burning of frankincense and myrrh in places of worship for spiritual purposes and contemplation (a ubiquitous practice across various religions) had hygienic functions, to refine the smell and reduce contagion by purifying the indoor air...
March 9, 2018: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/29529970/antibacterial-effects-of-18-medicinal-plants-used-by-the-khyang-tribe-in-bangladesh
#14
Md Shahadat Hossan, Hassan Jindal, Sarah Maisha, Chandramathi Samudi Raju, Shamala Devi Sekaran, Veeranoot Nissapatorn, Fatima Kaharudin, Lim Su Yi, Teng Jin Khoo, Mohammed Rahmatullah, Christophe Wiart
CONTEXT: The resistance of bacteria to antibiotics is raising serious concern globally. Asian medicinal plants could improve the current treatment strategies for bacterial infections. The antibacterial properties of medicinal plants used by the Khyang tribe in Bangladesh have not been investigated. OBJECTIVE: The present study examines the antibacterial properties of 18 medicinal plants used by the Khyang tribe in day-to-day practice against human pathogenic bacteria...
December 2018: Pharmaceutical Biology
https://www.readbyqxmd.com/read/29523309/quercetin-3-o-glucoside-recovered-from-the-wild-egyptian-sahara-plant-euphorbia-paralias-l-inhibits-glutamine-synthetase-and-has-antimycobacterial-activity
#15
Nesreen A Safwat, Mona T Kashef, Ramy K Aziz, Khadiga F Amer, Mohammed A Ramadan
Tuberculosis remains a major health problem accentuated by the rise of resistance to all available drugs. Therefore, this study was launched to discover a novel antituberculosis agent from wild Egyptian Sahara plants. Twelve such plants were screened, in vitro, for their activity against various Mycobacterium species. The most active plant, Euphorbia paralias, was further fractionated with different organic solvents, and the activity of the obtained fractions was determined by the agar diffusion and broth microdilution methods...
January 2018: Tuberculosis
https://www.readbyqxmd.com/read/29514937/an-improved-medium-for-colistin-susceptibility-testing
#16
Konrad Gwozdzinski, Saina Azarderakhsh, Can Imirzalioglu, Linda Falgenhauer, Trinad Chakraborty
The plasmid-located colistin resistance gene ( mcr - 1 ) confers low-level resistance to colistin, a last-line antibiotic against multidrug-resistant Gram-negative bacteria. Current EUCAST/CLSI recommendations require the use of a broth microdilution (BMD) method in cation-adjusted Mueller-Hinton (CA-MH) for colistin susceptibility testing, but approximately 15% of all MCR-1-producers classify as sensitive in this medium. Here we report on an improved calcium-enhanced Mueller-Hinton (CE-MH) medium that permits simple and reliable determination of mcr-1 -producing Enterobacteriaceae Colistin susceptibility for 50 mcr-1 -encoding isolates of E...
March 7, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29510602/evaluation-of-antimycobacterial-and-synergistic-activity-of-plants-selected-based-on-cheminformatic-parameters
#17
Nafise Rahgozar, Gholamreza Bakhshi Khaniki, Soroush Sardari
Background: Drug resistance is a major public health problem and a threat to progress made in bovine tuberculosis care and control worldwide. This study aimed at evaluating anti-mycobacterial and synergistic activity of some medicinal plants that were selected by cheminformatics studies against Mycobacterium bovis. Methods: Considering the strong synergistic antimycobacterial action of oleanolic acid in combination with tuberculosis drugs, NCBI database was explored to find the compounds with over 80% similarity to oleanolic acid, called S1...
March 7, 2018: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/29506044/echinocandin-resistance-among-candida-isolates-at-an-academic-medical-centre-2005-15-analysis-of-trends-and-outcomes
#18
Todd P McCarty, Shawn R Lockhart, Stephen A Moser, Jennifer Whiddon, Joanna Zurko, Cau D Pham, Peter G Pappas
Objectives: To identify the frequency of micafungin resistance among clinically significant isolates of Candida stored at our institution from 2005 to 2015. Chart review of patients with resistant isolates then informed the clinical setting and outcomes associated with these infections. Methods: Clinical Candida isolates had been stored at -80°C in Brucella broth with 20% glycerol from 2005. Isolates were tested using broth microdilution to determine micafungin MICs...
February 28, 2018: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29504922/evaluation-of-in-vitro-antimicrobial-activity-of-artemisia-apiacea-h-and-scutellaria-baicalensis-g-extracts
#19
Huan Trinh, Youngchul Yoo, Kyung-Hwa Won, Hien T T Ngo, Jung-Eun Yang, Jin-Gyeong Cho, Sang-Won Lee, Ki-Young Kim, Tae-Hoo Yi
PURPOSE: In traditional Korean medicine, Artemisia apiacea H. (ART) and Scutellaria baicalensis G. (SCU) are combined for the treatment of malaria and other malaria-like diseases. Because SCU is well-known as an antibacterial agent, the antimicrobial effect of a mixture of ART and SCU was investigated. METHODOLOGY: Plant samples were purchased from Kyungdong mart and extracted with 70 % ethanol. The in vitro antimicrobial activity of ART and SCU against pathogenic fungi (Aspergillus niger, Aspergillus oryzae, Candida albicans, Candida tropicalis and Candida glabrata), Gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa) and Gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus) was evaluated using a broth microdilution assay, modified-disc diffusion and agar dilution methods with further CH2Cl2-fractionated ART, SCU and a mixture of ART/SCU (at a ratio of 3 : 5) (THAN-1)...
March 5, 2018: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/29497926/antifungal-activity-of-chitosan-coated-poly-lactic-co-glycolic-acid-nanoparticles-containing-amphotericin-b
#20
Daniel Brustolin Ludwig, Luciana Erzinger Alves de Camargo, Najeh Maissar Khalil, Marcos Ereno Auler, Rubiana Mara Mainardes
Amphotericin B (AmB) is one of the most used drugs for the treatment of systemic fungal infections; however, the treatment causes several toxic manifestations, including nephrotoxicity and hemolytic anemia. Chitosan-coated poly(lactide-co-glycolide) (PLGA) nanoparticles containing AmB were developed with the aim to decrease AmB toxicity and propose the oral route for AmB delivery. In this work, the antifungal efficacy of chitosan-coated PLGA nanoparticles containing AmB was evaluated in 20 strains of fungus isolates from patients with vulvovaginal candidiasis (01 Candida glabrata and 03 Candida albicans), bloodstream infections (04 C...
March 1, 2018: Mycopathologia
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