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Microdilution assay

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https://www.readbyqxmd.com/read/28335779/activity-of-amp2041-against-human-and-animal-multidrug-resistant-pseudomonas-aeruginosa-clinical-isolates
#1
Clotilde Silvia Cabassi, Andrea Sala, Davide Santospirito, Giovanni Loris Alborali, Edoardo Carretto, Giovanni Ghibaudo, Simone Taddei
BACKGROUND: Antimicrobial resistance is a growing threat to public health. Pseudomonas aeruginosa is a relevant pathogen causing human and animal infections, frequently displaying high levels of resistance to commonly used antimicrobials. The increasing difficulty to develop new effective antibiotics have discouraged investment in this area and only a few new antibiotics are currently under development. An approach to overcome antibiotic resistance could be based on antimicrobial peptides since they offer advantages over currently used microbicides...
March 23, 2017: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/28333340/evaluation-of-the-new-genotype-ntm-dr-kit-for-the-molecular-detection-of-antimicrobial-resistance-in-non-tuberculous-mycobacteria
#2
Faiza Mougari, Jade Loiseau, Nicolas Veziris, Christine Bernard, Béatrice Bercot, Wladimir Sougakoff, Vincent Jarlier, Laurent Raskine, Emmanuelle Cambau
Objectives: Non-tuberculous mycobacteria (NTM) are emerging pathogens causing difficult-to-treat infections. We tested a new assay (GenoType NTM-DR) that detects natural and acquired resistance mechanisms to macrolides and aminoglycosides in frequently isolated NTM species. Methods: Performance was assessed on 102 isolates including reference strains [16 Mycobacterium avium , 10 Mycobacterium intracellulare , 8 Mycobacterium chimaera , 15 Mycobacterium chelonae and 53 Mycobacterium abscessus (including subsp...
February 28, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28333192/susceptibilities-of-mdr-mycobacterium-tuberculosis-isolates-to-unconventional-drugs-compared-with-their-reported-pharmacokinetic-pharmacodynamic-parameters
#3
Joseph S Cavanaugh, Ruwen Jou, Mei-Hua Wu, Tracy Dalton, Ekaterina Kurbatova, Julia Ershova, J Peter Cegielski
Background: The second-line drugs recommended to treat drug-resistant TB are toxic, expensive and difficult to procure. Given increasing resistance, the need for additional anti-TB drugs has become more urgent. But new drugs take time to develop and are expensive. Some commercially available drugs have reported anti-mycobacterial activity but are not routinely used because supporting laboratory and clinical evidence is sparse. Methods: We analysed 217 MDR M. tuberculosis isolates including 153 initial isolates from unique patients and 64 isolates from follow-up specimens during the course of treatment...
February 20, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28317391/in-vitro-inhibitory-effects-of-farnesol-and-interactions-between-farnesol-and-antifungals-against-biofilms-of-candida-albicans-resistant-strains
#4
Jinping Xia, Fang Qian, Wenqian Xu, Zhenzhen Zhang, Xin Wei
Antifungal resistance is a serious problem in clinical infections. Farnesol, which is a potential antifungal agent against biofilms formed by Candida albicans resistant strains (a fluconazole-resistant isolate derived from SC5314 and two clinical Candida resistant isolates), was investigated in this study. The inhibitory effects of farnesol on biofilms were examined by XTT assay. The morphological changes and biofilm thicknesses were analyzed by scanning electron microscopy and confocal laser scanning microscopy, respectively...
March 20, 2017: Biofouling
https://www.readbyqxmd.com/read/28304328/bactericidal-effect-of-pterostilbene-alone-and-in-combination-with-gentamicin-against-human-pathogenic-bacteria
#5
Wee Xian Lee, Dayang Fredalina Basri, Ahmad Rohi Ghazali
The antibacterial activity of pterostilbene in combination with gentamicin against six strains of Gram-positive and Gram-negative bacteria were investigated. The minimum inhibitory concentration and minimum bactericidal concentration of pterostilbene were determined using microdilution technique whereas the synergistic antibacterial activities of pterostilbene in combination with gentamicin were assessed using checkerboard assay and time-kill kinetic study. Results of the present study showed that the combination effects of pterostilbene with gentamicin were synergistic (FIC index < 0...
March 17, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28303159/antifungal-activity-of-the-ethanol-extract-from-flos-rosae-chinensis-with-activity-against-fluconazole-resistant-clinical-candida
#6
Lulu Zhang, Hui Lin, Wei Liu, Baodi Dai, Lan Yan, YongBing Cao, Yuan-Ying Jiang
This study was designed to investigate the antifungal activity of a hydroalcoholic extract from Flos Rosae Chinensis (FRC) combined with fluconazole (FCZ) against clinical isolates of Candida albicans resistant to FCZ. The minimum inhibitory concentration (MIC) of FRC was determined using a checkerboard microdilution assay. The synergistic effects of the combination of FRC and FCZ against clinical isolates of C. albicans resistant to FCZ were further confirmed by constructing time-growth curves and performing an agar diffusion test...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/28297749/endophytic-diaporthe-sp-ed2-produces-a-novel-anti-candidal-ketone-derivative
#7
Woei Yenn Tong, Chean Ring Leong, Wen Nee Tan, Melati Khairuddean, Latiffah Zakaria, Darah Ibrahim
This study was aimed to ascertain the anti-candidal efficacy of a novel ketone derivative isolated from Diaporthe sp. ED2, an endophytic fungus residing in medicinal herb Orthosiphon stamieus Benth. The ethyl acetate extract of the fungal culture was separated by open column and reverse phase- high performance liquid chromatography. The eluent of the second peak at retention time 5.64 minute in the HPLC system was the only peak that exhibited anti-candidal activity on Kirby Bauer assay. The structure of the compound was also elucidated by nuclear magnetic resonance and spectroscopy techniques...
March 15, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28286459/antimicrobial-properties-of-copper-nanoparticles-and-amino-acid-chelated-copper-nanoparticles-produced-by-using-a-soya-extract
#8
I DeAlba-Montero, Jesús Guajardo-Pacheco, Elpidio Morales-Sánchez, Rene Araujo-Martínez, G M Loredo-Becerra, Gabriel-Alejandro Martínez-Castañón, Facundo Ruiz, M E Compeán Jasso
This paper reports a comparison of the antibacterial properties of copper-amino acids chelates and copper nanoparticles against Escherichia coli, Staphylococcus aureus, and Enterococcus faecalis. These copper-amino acids chelates were synthesized by using a soybean aqueous extract and copper nanoparticles were produced using as a starting material the copper-amino acids chelates species. The antibacterial activity of the samples was evaluated by using the standard microdilution method (CLSI M100-S25 January 2015)...
2017: Bioinorganic Chemistry and Applications
https://www.readbyqxmd.com/read/28281322/chemical-composition-antibacterial-antibiofilm-and-synergistic-properties-of-essential-oils-from-eucalyptus-globulus-labill-and-seven-mediterranean-aromatic-plants
#9
Maria Vieira, Lucinda J Bessa, M Rosário Martins, Sílvia Arantes, António P S Teixeira, Ângelo Mendes, Paulo Martins da Costa, Anabela D F Belo
Essential oils (EOs) from Eucalyptus globulus Labill. ssp. globulus and from Mediterranean autochthonous aromatic plants - Thymus mastichina L., Mentha pulegium L., Rosmarinus officinalis L., Calamintha nepeta (L.) Savi ssp. nepeta, Cistus ladanifer L., Foeniculum vulgare L., Dittrichia viscosa (L.) Greuter ssp. viscosa - were extracted by hydrodistillation and characterized by GC-FID and NMR spectroscopy. EOs were evaluated for antimicrobial properties against several bacterial strains, using diverse methods, namely, the agar disc-diffusion method, the microdilution method, the crystal violet assay and the Live/Dead staining for assessment of biofilm formation...
March 9, 2017: Chemistry & Biodiversity
https://www.readbyqxmd.com/read/28277681/hormonemate-derivatives-from-dothiora-sp-an-endophytic-fungus
#10
Mercedes Pérez-Bonilla, Víctor González-Menéndez, Ignacio Pérez-Victoria, Nuria de Pedro, Jesús Martín, Joaquín Molero-Mesa, Manuel Casares-Porcel, María Reyes González-Tejero, Francisca Vicente, Olga Genilloud, José R Tormo, Fernando Reyes
A search for cytotoxic agents from cultures of the endophytic fungus Dothiora sp., isolated from the endemic plant Launaea arborescens, led to the isolation of six new compounds structurally related to hormonemate, with moderate cytotoxic activity against different cancer cell lines. By using a bioassay-guided fractionation approach, hormonemates A-D (1-4), hormonemate (5), and hormonemates E (6) and F (7) were obtained from the acetone extract of this fungus. Their structures were determined using a combination of HRMS, ESI-qTOF-MS/MS, 1D and 2D NMR experiments, and chemical degradation...
March 9, 2017: Journal of Natural Products
https://www.readbyqxmd.com/read/28274226/evaluation-of-the-antimycobacterial-activity-of-crude-extracts-and-solvent-fractions-of-selected-ethiopian-medicinal-plants
#11
Wubayehu Kahaliw, Abraham Aseffa, Markos Abebe, Mekonen Teferi, Ephrem Engidawork
BACKGROUND: Tuberculosis (TB) is a global health problem complicated by drug resistance and human immunodeficiency virus that has dramatically increased active TB. Several medicinal plants are used traditionally to treat TB in Ethiopia and investigating these plants is required as plants are an alternative source for development of new anti-TB drugs. The purpose of this study was to investigate antimycobacterial activity of crude extract of Carissa edulis, Otostegia integrifolia, Persea americana, Pterolobium stellatum and Vernonia amygdalina as well as fractions of the most active crude extract...
March 8, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28264849/genetic-diversity-and-in-vitro-antifungal-susceptibility-of-200-clinical-and-environmental-aspergillus-flavus-isolates
#12
Mojtaba Taghizadeh-Armaki, Mohammad Taghi Hedayati, Saham Ansari, Saeed Mahdavi Omran, Sasan Saber, Haleh Rafati, Jan Zoll, Henrich A van der Lee, Willem J G Melchers, Paul E Verweij, Seyedmojtaba Seyedmousavi
Aspergillus flavus has been frequently reported as the leading cause of invasive aspergillosis in certain tropical and sub-tropical countries.Two hundred A. flavus strains originating from clinical and environmental sources and collected between 2008 and 2015 were phylogenetically identified at the species level by analyzing partial β-tubulin and calmodulin genes. In vitro antifungal susceptibility testing was performed against antifungals using the EUCAST (European Committee on Antimicrobial Susceptibility Testing) broth microdilution method...
March 6, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28264518/synthesis-x-ray-single-crystal-structure-molecular-docking-and-dft-computations-on-n-1e-1-2h-1-3-benzodioxol-5-yl-3-1h-imidazol-1-yl-propylidene-hydroxylamine-a-new-potential-antifungal-agent-precursor
#13
Reem I Al-Wabli, Alwah R Al-Ghamdi, Hazem A Ghabbour, Mohamed H Al-Agamy, James Clemy Monicka, Issac Hubert Joe, Mohamed I Attia
Mycoses are serious health problem, especially in immunocompromised individuals. A new imidazole-bearing compound containing an oxime functionality was synthesized and characterized with different spectroscopic techniques to be used for the preparation of new antifungal agents. The stereochemistry of the oxime double bond was unequivocally determined via the single crystal X-ray technique. The title compound 4, C13H13N₃O₃·C₃H₈O, crystallizes in the monoclinic space group P2₁with a = 9.0963(3) Å, b = 14...
February 28, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28238773/inhibition-of-the-tetk-efflux-pump-by-the-essential-oil-of-chenopodium-ambrosioides-l-and-%C3%AE-terpinene-against-staphylococcus-aureus-is-58
#14
Paulo W Limaverde, Fábia F Campina, Francisco A B da Cunha, Francidalva D Crispim, Fernando G Figueredo, Luciene F Lima, Cícera Datiane de M Oliveira-Tintino, Yedda M L S de Matos, Maria Flaviana B Morais-Braga, Irwin R A Menezes, Valdir Q Balbino, Henrique D M Coutinho, José P Siqueira-Júnior, Jackson R G S Almeida, Saulo R Tintino
The use of natural products is crucial to suppress the development of these micro-organisms and to reduce the concentration necessary to inhibit these microrganisms, reducing the toxicity risks also. In this study, the essential oil from Chenopodium ambrosioides Leaves and its main constituent α-Terpinene were used in the antibacterial and potentiating activity of antibiotics and ethidium bromide assays, against the bacterial strains Staphylococcus aureus IS-58, carriers of efflux pumps. The Minimum Inhibitory Concentration (MIC) was determined using a microdilution method...
February 23, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28226293/physico-chemical-characterization-and-antibacterial-activity-of-inclusion-complexes-of-hyptis-martiusii-benth-essential-oil-in-%C3%AE-cyclodextrin
#15
Tatianny A Andrade, Thiago S Freitas, Francielly O Araújo, Paula P Menezes, Grace Anne A Dória, Alessandra S Rabelo, Lucindo J Quintans-Júnior, Márcio R V Santos, Daniel P Bezerra, Mairim R Serafini, Irwin Rose A Menezes, Paula Santos Nunes, Adriano A S Araújo, Maria S Costa, Fábia F Campina, Antonia T L Santos, Ana R P Silva, Henrique D M Coutinho
Cyclodextrins (CDs) have been used as important pharmaceutical excipients for improve the physicochemical properties of the drugs of low solubility as the essential oil of Hyptis martiusii. This oil is important therapeutically, but the low solubility and bioavailability compromises your use. Therein, the aim of this study was to obtain and to characterize physico-chemically the samples obtained by physical mixture (PM), paste complexation (PC) and slurry complexation (SC) of the essential oil Hyptis martiusii (EOHM) in β-CD, and to compare the antibacterial and modulatory-antibiotic activity of products obtained and oil free...
February 19, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28225903/sphygmomanometers-and-thermometers-as-potential-fomites-of-staphylococcus-haemolyticus-biofilm-formation-in-the-presence-of-antibiotics
#16
Bruna Pinto Ribeiro Sued, Paula Marcele Afonso Pereira, Yuri Vieira Faria, Juliana Nunes Ramos, Vanessa Batista Binatti, Kátia Regina Netto Dos Santos, Sérgio Henrique Seabra, Raphael Hirata, Verônica Viana Vieira, Ana Luíza Mattos-Guaraldi, José Augusto Adler Pereira
BACKGROUND: The association between Staphylococcus haemolyticus and severe nosocomial infections is increasing. However, the extent to which fomites contribute to the dissemination of this pathogen through patients and hospital wards remains unknown. OBJECTIVES: In the present study, sphygmomanometers and thermometers were evaluated as potential fomites of oxacillin-resistant S. haemolyticus (ORSH). The influence of oxacillin and vancomycin on biofilm formation by ORSH strains isolated from fomites was also investigated...
March 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28225897/sphygmomanometers-and-thermometers-as-potential-fomites-of-staphylococcus-haemolyticus-biofilm-formation-in-the-presence-of-antibiotics
#17
Bruna Pinto Ribeiro Sued, Paula Marcele Afonso Pereira, Yuri Vieira Faria, Juliana Nunes Ramos, Vanessa Batista Binatti, Kátia Regina Netto Dos Santos, Sérgio Henrique Seabra, Raphael Hirata, Verônica Viana Vieira, Ana Luíza Mattos-Guaraldi, José Augusto Adler Pereira
BACKGROUND: The association between Staphylococcus haemolyticus and severe nosocomial infections is increasing. However, the extent to which fomites contribute to the dissemination of this pathogen through patients and hospital wards remains unknown. OBJECTIVES: In the present study, sphygmomanometers and thermometers were evaluated as potential fomites of oxacillin-resistant S. haemolyticus (ORSH). The influence of oxacillin and vancomycin on biofilm formation by ORSH strains isolated from fomites was also investigated...
February 16, 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28223069/evaluation-of-antibacterial-potential-and-toxicity-of-plant-volatile-compounds-using-new-broth-microdilution-volatilization-method-and-modified-mtt-assay
#18
Marketa Houdkova, Johana Rondevaldova, Ivo Doskocil, Ladislav Kokoska
With aim to develop effective proof-of-concept approach which can be used in a development of new preparations for the inhalation therapy, we designed a new screening method for simple and rapid simultaneous determination of antibacterial potential of plant volatiles in the liquid and the vapour phase at different concentrations. In addition, EVA (ethylene vinyl acetate) capmat™ as vapour barrier cover was used as reliable modification of thiazolyl blue tetrazolium bromide (MTT) assay for cytotoxicity testing of volatiles on microtiter plates...
February 20, 2017: Fitoterapia
https://www.readbyqxmd.com/read/28219296/phytochemical-screening-and-evaluation-of-in-vitro-antioxidant-and-antimicrobial-activities-of-the-indigenous-medicinal-plant-albizia-odoratissima
#19
Venkanna Banothu, Chandrasekharnath Neelagiri, Uma Adepally, Jayalakshmi Lingam, Kesavaharshini Bommareddy
CONTEXT: Albizia odoratissima (L. f.) Benth has been used in Indian folk medicine to treat numerous inflammatory pathologies, such as leprosy, ulcers, burns and asthma. OBJECTIVE: To evaluate the antioxidant and antimicrobial properties of A. odoratissima. MATERIALS AND METHODS: Dried leaves of A. odoratissima were extracted in organic solvents (hexane, chloroform, ethyl acetate, and methanol). The total phenolic content (TPC) and total flavonoid content (TFC) were determined using the Folin-Ciocalteu and aluminum chloride colorimetric methods, respectively...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/28216880/isolation-and-characterisation-of-a-proanthocyanidin-with-antioxidative-antibacterial-and-anti-cancer-properties-from-fern-blechnum-orientale
#20
How-Yee Lai, Yau-Yan Lim, Kah-Hwi Kim
BACKGROUND: Blechnum orientale Linn. (Blechnaceae), a fern, is traditionally used in the treatment of various ailments, such as skin diseases, stomach pain, urinary bladder complaints, and also as a female contraceptive. Previously, we reported a strong radical scavenging activity, antibacterial activity and cytotoxicity against HT29 colon cancer cells by aqueous extract of B. orientale. OBJECTIVE: In this study, we attempted to isolate and identify the active compound from the aqueous extract of B...
January 2017: Pharmacognosy Magazine
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