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Microdilution assay

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https://www.readbyqxmd.com/read/28821717/promysalin-is-a-salicylate-containing-antimicrobial-with-a-cell-membrane-disrupting-mechanism-of-action-on-gram-positive-bacteria
#1
Rahul D Kaduskar, Giulia Della Scala, Zaaima J H Al Jabri, Stefania Arioli, Loana Musso, Marco R Oggioni, Sabrina Dallavalle, Diego Mora
Promysalin was previously described as a narrow spectrum molecule with a unique species-specific activity against Pseudomonas aeruginosa. Here we demonstrate that promysalin is active against Gram-positive and Gram-negative bacteria using a microdilution assay. Promysalin acts on Gram-positive bacteria with a mechanism of action involving cell membrane damage with leakage of intracellular components. The evaluation of MICs and MBCs on 11 promysalin analogs, synthesized utilizing diverted total synthesis, allowed the identification of the structural moieties potentially involved in cell membrane interaction and damage...
August 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28815601/antimicrobial-synergism-and-cytotoxic-properties-of-citrus-limon-l-piper-nigrum-l-and-melaleuca-alternifolia-maiden-and-betche-cheel-essential-oils
#2
Miloš M Nikolić, Katarina K Jovanović, Tatjana Lj Marković, Dejan Lj Marković, Nevenka N Gligorijević, Siniša S Radulović, Marina Kostić, Jasmina M Glamočlija, Marina D Soković
OBJECTIVES: The chemical composition, antimicrobial and synergistic effect, and cytotoxic activity of Citrus limon (lemon), Piper nigrum (green pepper) and Melaleuca alternifoila (tea tree) essential oils (EOs) were investigated. METHODS: Chemical analyses of essential oils were tested by GC-FID and GC-MS spectroscopy. The antimicrobial activity assay was conducted using microdilution method against several oral bacteria and Candida spp. originating from the humans with oral disorders...
August 17, 2017: Journal of Pharmacy and Pharmacology
https://www.readbyqxmd.com/read/28802274/horizontal-dissemination-of-antimicrobial-resistance-determinants-in-multiple-salmonella-serotypes-following-isolation-from-the-environment-of-commercial-swine-operations-after-manure-application
#3
Suchawan Pornsukarom, Siddhartha Thakur
The aim of this study was to characterize the plasmids carrying antimicrobial resistance (AMR) determinants in multiple Salmonella serotypes recovered from the commercial swine farms environment after manure application on land. Manure and soil samples were collected on day0 before and after manure application on six farms in North Carolina and sequential soil samples were recollected on day7, 14, and 21 from the same plots. All environmental samples were processed for Salmonella and their plasmid contents were further characterized...
August 11, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28797303/combined-antioxidant-effects-of-neem-extract-bacteria-red-blood-cells-and-lysozyme-possible-relation-to-periodontal-disease
#4
Leali Heyman, Yael Houri-Haddad, Samuel N Heyman, Isaac Ginsburg, Yossi Gleitman, Osnat Feuerstein
BACKGROUND: The common usage of chewing sticks prepared from Neem tree (Azadirachta indica) in India suggests its potential efficacy in periodontal diseases. The objective of this study is to explore the antibacterial effects of Neem leaf extract on the periodontophatic bacteria Porphyromonas gingivalis and Fusobacterium nucleatum, and its antioxidant capacities alone and in combination with bacteria and polycationic peptides that may be at the site of inflammation. METHODS: Neem leaf extract was prepared by ethanol extraction...
August 10, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28795907/synergy-of-a-combination-of-nisin-and-citric-acid-against-staphylococcus-aureus-and-listeria-monocytogenes
#5
Xingchen Zhao, Zhen Zhen, Xinyang Wang, Na Guo
Food-borne diseases caused by pathogens, such as Staphylococcus aureus and Listeria monocytogenes, have for a long time attracted intensive worldwide attention from scientific researchers, food industries, and food safety authorities. Nisin (NS) is the only bacteriocin used worldwide as a GRAS food preservative, while citric acid (CA) has an unrestricted use in foods since it has GRAS status. In this study, synergistic interactions of NS combined with CA against S. aureus and L. monocytogenes were studied by the checkerboard microdilution method, with fractional inhibitory concentration index values ranging from 0...
August 10, 2017: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
https://www.readbyqxmd.com/read/28793029/chemical-composition-antimicrobial-and-antioxidant-potential-of-the-essential-oil-of-guarea-kunthiana-a-juss
#6
J A Pandini, F G S Pinto, M C Scur, C B Santana, W F Costa, L G Temponi
The essential oils are extracted from plant compounds and can present activities antimicrobial and antioxidant properties. The goals of the present study were: (a) to determine the chemical composition of the essential oil of Guarea kunthiana A. Juss using the method of gas chromatography coupled to mass spectrometry (GC-MS); (b) to evaluate the antimicrobial potential of this oil using the broth microdilution method against different microorganisms: five Gram-negative bacteria, four Gram-positive bacteria and a yeast and (c) to determine the antioxidant activity of the oil using the DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical assay...
July 27, 2017: Brazilian Journal of Biology, Revista Brasleira de Biologia
https://www.readbyqxmd.com/read/28791824/anti-enterococcal-activities-of-pentacyclic-triterpenes
#7
Dorota Wojnicz, Dorota Tichaczek-Goska, Kamila Korzekwa, Marta Kicia, Andrzej Hendrich
BACKGROUND: Asiatic (AA) and ursolic (UA) acids are widely studied phytochemicals, but their antimicrobial properties are still poorly understood. Therefore our research has focused on their activity against uropathogenic Enterococcus faecalis strains. OBJECTIVES: The aim of this research was to determine the influence of AA and UA on the growth, cell morphology, virulence factors and biofilm formation by E. faecalis strains. MATERIAL AND METHODS: AA and UA were purchased from Sigma-Aldrich...
May 2017: Advances in Clinical and Experimental Medicine: Official Organ Wroclaw Medical University
https://www.readbyqxmd.com/read/28782085/in-vivo-and-in-vitro-control-activity-of-plant-essential-oils-against-three-strains-of-aspergillus-niger
#8
Peeyush Kumar, Sapna Mishra, Atul Kumar, Sanjeev Kumar, Chandra Shekhar Prasad
Contamination of environment and food from the prevalent spores and mycotoxins of Aspergillus niger has led to several diseases in humans and other animals. The present study investigated the control activity of plant essential oils against three strains of A. niger. In the elaborate assays done through microdilution plate assay and agar disk diffusion assay in the lab condition and in vivo assay on the stored wheat grains, the essential oil of Thymus vulgaris depicted overall superior efficacy. In microdilution plate assay, the oil of Anethum graveolens showed best fungistatic activity, while best fungicidal activity was depicted by Syzygium aromaticum oil...
August 7, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28763988/screening-bioactivity-and-bioactive-constituents-of-nordic-unifloral-honeys
#9
Anneli Salonen, Virpi Virjamo, Päivi Tammela, Laure Fauch, Riitta Julkunen-Tiitto
The objective of this study was to screen the antibacterial and antioxidant activity of thirty nine honey samples from Finland, Sweden, Norway and Denmark. Their physicochemical properties were analysed, antioxidant activity was evaluated by DPPH assay and antibacterial activity against Pseudomonas aeruginosa and Staphylococcus aureus was assessed by microdilution assay. The honey samples obtained were buckwheat, caraway, clover, dandelion, fireweed, heather, lime tree, lingonberry, rape, raspberry, sweet clover, willow, mire, honeydew and polyfloral...
December 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28763743/synergistic-effects-of-some-essential-oils-against-fungal-spoilage-on-pear-fruit
#10
Mehdi Nikkhah, Maryam Hashemi, Mohammad B Habibi Najafi, Reza Farhoosh
The development of natural protective agents as alternatives to chemical fungicides is currently in the spotlight. In the present investigation, chemical composition and antifungal activities of thyme, cinnamon, rosemary and marjoram essential oils (EO), as well as synergism of their possible double and triple combinations were investigated. The compositions of the oils were determined by GC/MS. For determination of antifungal activity against Penicillium expansum and Botrytis cinerea, a broth microdilution method was used...
June 24, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28760709/antimicrobial-susceptibility-testing-of-kingella-kingae-with-broth-microdilution-and-disk-diffusion-using-eucast-recommended-media
#11
Erika Matuschek, Jenny Åhman, Gunnar Kahlmeter, Pablo Yagupsky
OBJECTIVES: Due to the increasing use of improved culture techniques and sensitive nucleic acid amplification assays, Kingella kingae was recognized as an important cause of invasive infections in young children, especially in septic arthritis, osteomyelitis, bacteremia and endocarditis. In 2016, EUCAST established clinical MIC breakpoints for K. kingae (published in EUCAST Clinical Breakpoint Tables v 7.0, 2017). The present study was carried out to produce MIC-zone diameter correlations for K...
July 28, 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/28751240/evaluation-of-vancomycin-mic-creep-in-staphylococcus-aureus-infections
#12
Raquel Diaz, Elmano Ramalheira, Vera Afreixo, Bruno Gago
BACKGROUND: Vancomycin is the primary treatment for meticillin-resistant Staphylococcus aureus (MRSA). However, increasing proportions of MRSA isolates with high MICs are being observed within the susceptible range (vancomycin MIC creep). The aim of this study was to assess vancomycin MIC distribution for S.aureus infections, over a period of four years, in Centro Hospitalar Baixo Vouga, Aveiro, Portugal and identify differences in vancomycin MIC through different susceptibility testing methods METHODS: For each isolate, the MIC of vancomycin for S...
July 24, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28748180/buwchitin-a-ruminal-peptide-with-antimicrobial-potential-against-enterococcus-faecalis
#13
Linda B Oyama, Jean-Adrien Crochet, Joan E Edwards, Susan E Girdwood, Alan R Cookson, Narcis Fernandez-Fuentes, Kai Hilpert, Peter N Golyshin, Olga V Golyshina, Florence Privé, Matthias Hess, Hilario C Mantovani, Christopher J Creevey, Sharon A Huws
Antimicrobial peptides (AMPs) are gaining popularity as alternatives for treatment of bacterial infections and recent advances in omics technologies provide new platforms for AMP discovery. We sought to determine the antibacterial activity of a novel antimicrobial peptide, buwchitin, against Enterococcus faecalis. Buwchitin was identified from a rumen bacterial metagenome library, cloned, expressed and purified. The antimicrobial activity of the recombinant peptide was assessed using a broth microdilution susceptibility assay to determine the peptide's killing kinetics against selected bacterial strains...
2017: Frontiers in Chemistry
https://www.readbyqxmd.com/read/28747232/development-of-an-accumulation-assay-and-evaluation-of-the-effects-of-efflux-pump-inhibitors-on-the-retention-of-chlorhexidine-digluconate-in-pseudomonas-aeruginosa-and-staphylococcus-aureus
#14
Molly Mombeshora, Stanley Mukanganyama
BACKGROUND: Chlorhexidine digluconate (CHG) is used as a disinfectant. The emergence of pathogens resistant to the biocide raises health concern. Information on specific efflux mechanisms utilised by bacteria to confer reduced susceptibility to the biocide, may be used to develop ways of preventing the efflux of the biocide from nosocomial pathogens resulting in higher disinfection activity. The aim of the study was to evaluate the role of ATP-binding cassette transporters on the transport of CHG in bacteria...
July 26, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/28747178/anaerobic-bacteria-growth-in-the-presence-of-cathelicidin-ll-37-and-selected-ceragenins-delivered-as-magnetic-nanoparticles-cargo
#15
Bonita Durnaś, Ewelina Piktel, Marzena Wątek, Tomasz Wollny, Stanisław Góźdź, Jolanta Smok-Kalwat, Katarzyna Niemirowicz, Paul B Savage, Robert Bucki
BACKGROUND: Cationic antibacterial peptides (CAPs) and synthetic molecules mimicking the amphiphilic structure of CAPs, such as ceragenins, are promising compounds for the development of new antimicrobials. RESULTS: We tested the in vitro activity of ceragenins CSA-13 and CSA-131 against several anaerobic bacteria including Bacteroides spp. and Clostridium difficile. We compared results to the activity of cathelicidin LL-37, metronidazole and nanosystems developed by attachment of CSA-13 and CSA-131 to magnetic nanoparticles (MNPs)...
July 26, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28746550/melipona-mondury-produces-a-geopropolis-with-antioxidant-antibacterial-and-antiproliferative-activities
#16
Tássia L A Dos Santos, Raphael F Queiroz, Alexandra C H F Sawaya, Begoña Gimenez-Cassina Lopez, Milena B P Soares, Daniel P Bezerra, Ana Carolina B C Rodrigues, Vanderlúcia F DE Paula, Ana Maria Waldschmidt
Geopropolis is a special type of propolis produced by stingless bees. Several pharmacological properties have been described for different types of geopropolis, but there have been no previous studies of the geopropolis from Melipona mondury. In this study, we investigated the antioxidant, antibacterial, and antiproliferative activities of M. mondury geopropolis, and determined its chemical profile. The antioxidant activity was determined using in vitro ABTS·+, ·DPPH, and β-carotene/linoleic acid co-oxidation methods...
July 20, 2017: Anais da Academia Brasileira de Ciências
https://www.readbyqxmd.com/read/28744918/essential-oils-and-hydrophilic-extracts-from-the-leaves-and-flowers-of-succisa-pratensis-moench-and-their-biological-activity
#17
Ewa Witkowska-Banaszczak, Jolanta Długaszewska
OBJECTIVES: This study was undertaken to evaluate the antioxidant activity of methanol and water extracts from Succisa pratensis Moench (Dipsacaceae) leaves and flowers as well as the chemical composition of the essential oils found in them and the antimicrobial activity of the oils and extracts thereof. METHODS: The essential oils from S. pratensis leaves and flowers were analysed by the GC-MS. The total phenolic content was determined with Folin-Ciocalteu, that of flavonoids with aluminium chloride and that of phenolic acids with Arnov's reagent...
July 26, 2017: Journal of Pharmacy and Pharmacology
https://www.readbyqxmd.com/read/28743086/effect-of-cinnamon-cinnamomum-verum-bark-essential-oil-on-the-halitosis-associated-bacterium-solobacterium-moorei-and-in-vitro-cytotoxicity
#18
Geneviève LeBel, Bruno Haas, Andrée-Ann Adam, Marie-Pier Veilleux, Amel Ben Lagha, Daniel Grenier
OBJECTIVES: Halitosis, also known as bad breath or oral malodour, is a condition affecting a large proportion of the population. Solobacterium moorei is a Gram-positive anaerobic bacterium that has been specifically associated with halitosis. In this study, we investigated the effects of essential oils, more particularly cinnamon bark oil, on growth, biofilm formation, eradication and killing, as well as hydrogen sulfide (H2S) production by S. moorei. METHODS: A broth microdilution assay was used to determine the antibacterial activity of essential oils...
July 12, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28741096/reproducible-measurement-of-vancomycin-mics-within-the-susceptible-range-in-staphylococcus-aureus-by-a-broth-microdilution-method-with-a-quasi-continuum-gradient-of-antibiotic-concentrations
#19
R Falcón, E M Mateo, A Talaya, E Giménez, V Vinuesa, M Á Clari, D Navarro
The availability of reproducible broth microdilution (BMD) methods including inter log2 antibiotic dilutions for measuring Staphylococcus aureus (SA) vancomycin minimum inhibitory concentrations (MICs) within the susceptible range is needed to elucidate the impact of vancomycin MICs on clinical outcomes of invasive SA infections. Here, we report on the development of a very precise BMD method that incorporates the following incremental antibiotic concentrations: 0.50, 0.62, 0.75, 0.87, 1.0, 1.25, 1.40, 1.50, 1...
July 25, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28738715/antimicrobial-modulatory-and-chemical-analysis-of-the-oil-of-croton-limae
#20
Tiago Rodrigues Leite, Maria Arlene Pessoa da Silva, Antônio Carlito Bezerra Dos Santos, Henrique Douglas Melo Coutinho, Antonia Eliene Duarte, José Galberto Martins da Costa
CONTEXT: Croton sp. are plants with a well-reported antimicrobial activity. Croton limae A.P. Gomes, M.F. Sales P.E. Berry (Euphorbiaceae), known as 'marmeleiro-prateado', is commonly used to manage abdominal pain in Brazil. OBJECTIVE: This work evaluates the phytochemical composition, antimicrobial and modulatory activities of the essential oil of C. limae leaves (EOCL). MATERIALS AND METHODS: The minimum inhibitory concentration (MIC) and the modulation of the antibiotic activity were determined using a microdilution method...
December 2017: Pharmaceutical Biology
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